TBX3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TBX3
Entrez Gene ID	6926
Full Name	T-box 3
Synonyms	TBX3-ISO,UMS,XHL
General protein information	Preferred Names T-box 3 Names T-box transcription factor TBX3 T-box protein 3 bladder cancer related protein XHL
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.21
Summary	This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This protein is a transcriptional repressor and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Mutations in this gene cause ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing of this gene results in three transcript variants encoding different isoforms; however, the full length nature of one variant has not been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	601621
Disorder Html:	Ulnar-mammary syndrome, 181450 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_016569.3	NP_057653.3	T-box transcription factor TBX3 isoform 2
NM_005996.3	NP_005987.3	T-box transcription factor TBX3 isoform 1
NM_016569.4	NP_057653.3	T-box transcription factor TBX3 isoform 2
NM_005996.4	NP_005987.3	T-box transcription factor TBX3 isoform 1

Pathways from BioSystems

Homologies

	TBX3	XP_001111920.1
Bos taurus (cattle)	TBX3	XP_002694634.3
Xenopus tropicalis (tropical clawed frog)	tbx3	NP_001027524.1
Pan troglodytes (chimpanzee)	TBX3	XP_001154426.1
Canis lupus familiaris (dog)	TBX3	XP_005636571.1
Mus musculus (house mouse)	Tbx3	NP_035665.2
Rattus norvegicus (Norway rat)	Tbx3	NP_853669.1
Danio rerio (zebrafish)	tbx3a	NP_001095140.2
Gallus gallus (chicken)	TBX3	NP_001257807.1
Homo sapiens (human)	TBX3	NP_057653.3

Gene Ontology
Bibliography

Related articles in PubMed

T-box transcription factor Tbx3 contributes to human hepatocellular carcinoma cell migration and invasion by repressing E-cadherin expression.
Feng X, Yao W, Zhang Z, Yuan F, Liang L, Zhou J, Liu S, Song J
Oncology research(2018 Jan)

Novel TBX3 mutation in a family of Cypriot ancestry with ulnar-mammary syndrome.
Tanteles GA, Nicolaou N, Syrimis A, Metaxa R, Nicolaou M, Christophidou-Anastasiadou V, Skordis N
Clinical dysmorphology26(2)61-65(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The c.1423C>T (p.Q475*) nonsense mutation in exon 6 of the TBX3 gene associated with ulnar-mammary syndrome.
Title: Novel TBX3 mutation in a family of Cypriot ancestry with ulnar-mammary syndrome.

Tbx3 was found to be overexpressed in 46 of 98 human gastric cancer samples
Title: Tbx3 overexpression in human gastric cancer is correlated with advanced tumor stage and nodal status and promotes cancer cell growth and invasion.

TBX3 is not only required for development of posterior forelimb bones (ulna and digits 4 and 5), but also for a subset of posterior muscles (lateral triceps and brachialis) and their bone eminence attachment sites.
Title: Development of a subset of forelimb muscles and their attachment sites requires the ulnar-mammary syndrome gene Tbx3.

High TBX3 expression is associated with invasive breast cancer.
Title: The transcriptional regulator TBX3 promotes progression from non-invasive to invasive breast cancer.

genetic variation of Tbx3 is associated with left ventricular mass in a healthy Japanese population [meta-analysis]
Title: Genome-Wide Association Study of Absolute QRS Voltage Identifies Common Variants of TBX3 as Genetic Determinants of Left Ventricular Mass in a Healthy Japanese Population.

The following TBX3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBX3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17127	NM_005996.4 Latest version!	Homo sapiens T-box transcription factor 3 (TBX3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu18626	NM_016569.4 Latest version!	Homo sapiens T-box transcription factor 3 (TBX3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu18626	NM_016569.3	Homo sapiens T-box 3 (TBX3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu17127	NM_005996.3	Homo sapiens T-box 3 (TBX3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TBX3 ( NM_005996.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005987.3 Homo sapiens T-box 3 (TBX3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17127
Clone ID Related Accession (Same CDS sequence)	NM_005996.3 , NM_005996.4
Accession Version	NM_005996.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	T-box transcription factor TBX3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK054604.1, BC025258.1 and AF140240.1. On May 17, 2004 this sequence version replaced NM_005996.2. Transcript Variant: This variant (1) encodes the shorter isoform (1) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.82626.1, AF170708.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267762 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTCT CCATGAGAGA TCCGGTCATT CCTGGGACAA GCATGGCCTA CCATCCGTTC 
CTACCTCACC GGGCGCCGGA CTTCGCCATG AGCGCGGTGC TGGGTCACCA GCCGCCGTTC 
TTCCCCGCGC TGACGCTGCC TCCCAACGGC GCGGCGGCGC TCTCGCTGCC GGGCGCCCTG 
GCCAAGCCGA TCATGGATCA ATTGGTGGGG GCGGCCGAGA CCGGCATCCC GTTCTCCTCC 
CTGGGGCCCC AGGCGCATCT GAGGCCTTTG AAGACCATGG AGCCCGAAGA AGAGGTGGAG 
GACGACCCCA AGGTGCACCT GGAGGCTAAA GAACTTTGGG ATCAGTTTCA CAAGCGGGGC 
ACCGAGATGG TCATTACCAA GTCGGGAAGG CGAATGTTTC CTCCATTTAA AGTGAGATGT 
TCTGGGCTGG ATAAAAAAGC CAAATACATT TTATTGATGG ACATTATAGC TGCTGATGAC 
TGTCGTTATA AATTTCACAA TTCTCGGTGG ATGGTGGCTG GTAAGGCCGA CCCCGAAATG 
CCAAAGAGGA TGTACATTCA CCCGGACAGC CCCGCTACTG GGGAACAGTG GATGTCCAAA 
GTCGTCACTT TCCACAAACT GAAACTCACC AACAACATTT CAGACAAACA TGGATTTACT 
ATATTGAACT CCATGCACAA ATACCAGCCC CGGTTCCACA TTGTAAGAGC CAATGACATC 
TTGAAACTCC CTTATAGTAC ATTTCGGACA TACTTGTTCC CCGAAACTGA ATTCATCGCT 
GTGACTGCAT ACCAGAATGA TAAGATAACC CAGTTAAAAA TAGACAACAA CCCTTTTGCA 
AAAGGTTTCC GGGACACTGG AAATGGCCGA AGAGAAAAAA GAAAACAGCT CACCCTGCAG 
TCCATGAGGG TGTTTGATGA AAGACACAAA AAGGAGAATG GGACCTCTGA TGAGTCCTCC 
AGTGAACAAG CAGCTTTCAA CTGCTTCGCC CAGGCTTCTT CTCCAGCCGC CTCCACTGTA 
GGGACATCGA ACCTCAAAGA TTTATGTCCC AGCGAGGGTG AGAGCGACGC CGAGGCCGAG 
AGCAAAGAGG AGCATGGCCC CGAGGCCTGC GACGCGGCCA AGATCTCCAC CACCACGTCG 
GAGGAGCCCT GCCGTGACAA GGGCAGCCCC GCGGTCAAGG CTCACCTTTT CGCTGCTGAG 
CGGCCCCGGG ACAGCGGGCG GCTGGACAAA GCGTCGCCCG ACTCACGCCA TAGCCCCGCC 
ACCATCTCGT CCAGCACTCG CGGCCTGGGC GCGGAGGAGC GCAGGAGCCC GGTTCGCGAG 
GGCACAGCGC CGGCCAAGGT GGAAGAGGCG CGCGCGCTCC CGGGCAAGGA GGCCTTCGCG 
CCGCTCACGG TGCAGACGGA CGCGGCCGCC GCGCACCTGG CCCAGGGCCC CCTGCCTGGC 
CTCGGCTTCG CCCCGGGCCT GGCGGGCCAA CAGTTCTTCA ACGGGCACCC GCTCTTCCTG 
CACCCCAGCC AGTTTGCCAT GGGGGGCGCC TTCTCCAGCA TGGCGGCCGC TGGCATGGGT 
CCCCTCCTGG CCACGGTTTC TGGGGCCTCC ACCGGTGTCT CGGGCCTGGA TTCCACGGCC 
ATGGCCTCTG CCGCTGCGGC GCAGGGACTG TCCGGGGCGT CCGCGGCCAC CCTGCCCTTC 
CACCTCCAGC AGCACGTCCT GGCCTCTCAG GGCCTGGCCA TGTCCCCTTT CGGAAGCCTG 
TTCCCTTACC CCTACACGTA CATGGCCGCA GCGGCGGCCG CCTCCTCTGC GGCAGCCTCC 
AGCTCGGTGC ACCGCCACCC CTTCCTCAAT CTGAACACCA TGCGCCCGCG GCTGCGCTAC 
AGCCCCTACT CCATCCCGGT GCCGGTCCCG GACGGCAGCA GTCTGCTCAC CACCGCCCTG 
CCCTCCATGG CGGCGGCCGC GGGGCCCCTG GACGGCAAAG TCGCCGCCCT GGCCGCCAGC 
CCGGCCTCGG TGGCAGTGGA CTCGGGCTCT GAACTCAACA GCCGCTCCTC CACGCTCTCC 
TCCAGCTCCA TGTCCTTGTC GCCCAAACTC TGCGCGGAGA AAGAGGCGGC CACCAGCGAA 
CTGCAGAGCA TCCAGCGGTT GGTTAGCGGC TTGGAAGCCA AGCCGGACAG GTCCCGCAGC 
GCGTCCCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005987.3
CDS	965..3136
Translation	MSLSMRDPVIPGTSMAYHPFLPHRAPDFAMSAVLGHQPPFFPALTLPPNGAAALSLPGALAKPIMDQLVGAAETGIPFSSLGPQAHLRPLKTMEPEEEVEDDPKVHLEAKELWDQFHKRGTEMVITKSGRRMFPPFKVRCSGLDKKAKYILLMDIIAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMSKVVTFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYLFPETEFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNGRREKRKQLTLQSMRVFDERHKKENGTSDESSSEQAAFNCFAQASSPAASTVGTSNLKDLCPSEGESDAEAESKEEHGPEACDAAKISTTTSEEPCRDKGSPAVKAHLFAAERPRDSGRLDKASPDSRHSPATISSSTRGLGAEERRSPVREGTAPAKVEEARALPGKEAFAPLTVQTDAAAAHLAQGPLPGLGFAPGLAGQQFFNGHPLFLHPSQFAMGGAFSSMAAAGMGPLLATVSGASTGVSGLDSTAMASAAAAQGLSGASAATLPFHLQQHVLASQGLAMSPFGSLFPYPYTYMAAAAAASSAAASSSVHRHPFLNLNTMRPRLRYSPYSIPVPVPDGSSLLTTALPSMAAAAGPLDGKVAALAASPASVAVDSGSELNSRSSTLSSSSMSLSPKLCAEKEAATSELQSIQRLVSGLEAKPDRSRSASP

Target ORF information:

RefSeq Version	NM_005996.3
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box 3 (TBX3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005996.3

ORF Insert Sequence:

ATGAGCCTCT CCATGAGAGA TCCGGTCATT CCTGGGACAA GCATGGCCTA CCATCCGTTC 
CTACCTCACC GGGCGCCGGA CTTCGCCATG AGCGCGGTGC TGGGTCACCA GCCGCCGTTC 
TTCCCCGCGC TGACGCTGCC TCCCAACGGC GCGGCGGCGC TCTCGCTGCC GGGCGCCCTG 
GCCAAGCCGA TCATGGATCA ATTGGTGGGG GCGGCCGAGA CCGGCATCCC GTTCTCCTCC 
CTGGGGCCCC AGGCGCATCT GAGGCCTTTG AAGACCATGG AGCCCGAAGA AGAGGTGGAG 
GACGACCCCA AGGTGCACCT GGAGGCTAAA GAACTTTGGG ATCAGTTTCA CAAGCGGGGC 
ACCGAGATGG TCATTACCAA GTCGGGAAGG CGAATGTTTC CTCCATTTAA AGTGAGATGT 
TCTGGGCTGG ATAAAAAAGC CAAATACATT TTATTGATGG ACATTATAGC TGCTGATGAC 
TGTCGTTATA AATTTCACAA TTCTCGGTGG ATGGTGGCTG GTAAGGCCGA CCCCGAAATG 
CCAAAGAGGA TGTACATTCA CCCGGACAGC CCCGCTACTG GGGAACAGTG GATGTCCAAA 
GTCGTCACTT TCCACAAACT GAAACTCACC AACAACATTT CAGACAAACA TGGATTTACT 
ATATTGAACT CCATGCACAA ATACCAGCCC CGGTTCCACA TTGTAAGAGC CAATGACATC 
TTGAAACTCC CTTATAGTAC ATTTCGGACA TACTTGTTCC CCGAAACTGA ATTCATCGCT 
GTGACTGCAT ACCAGAATGA TAAGATAACC CAGTTAAAAA TAGACAACAA CCCTTTTGCA 
AAAGGTTTCC GGGACACTGG AAATGGCCGA AGAGAAAAAA GAAAACAGCT CACCCTGCAG 
TCCATGAGGG TGTTTGATGA AAGACACAAA AAGGAGAATG GGACCTCTGA TGAGTCCTCC 
AGTGAACAAG CAGCTTTCAA CTGCTTCGCC CAGGCTTCTT CTCCAGCCGC CTCCACTGTA 
GGGACATCGA ACCTCAAAGA TTTATGTCCC AGCGAGGGTG AGAGCGACGC CGAGGCCGAG 
AGCAAAGAGG AGCATGGCCC CGAGGCCTGC GACGCGGCCA AGATCTCCAC CACCACGTCG 
GAGGAGCCCT GCCGTGACAA GGGCAGCCCC GCGGTCAAGG CTCACCTTTT CGCTGCTGAG 
CGGCCCCGGG ACAGCGGGCG GCTGGACAAA GCGTCGCCCG ACTCACGCCA TAGCCCCGCC 
ACCATCTCGT CCAGCACTCG CGGCCTGGGC GCGGAGGAGC GCAGGAGCCC GGTTCGCGAG 
GGCACAGCGC CGGCCAAGGT GGAAGAGGCG CGCGCGCTCC CGGGCAAGGA GGCCTTCGCG 
CCGCTCACGG TGCAGACGGA CGCGGCCGCC GCGCACCTGG CCCAGGGCCC CCTGCCTGGC 
CTCGGCTTCG CCCCGGGCCT GGCGGGCCAA CAGTTCTTCA ACGGGCACCC GCTCTTCCTG 
CACCCCAGCC AGTTTGCCAT GGGGGGCGCC TTCTCCAGCA TGGCGGCCGC TGGCATGGGT 
CCCCTCCTGG CCACGGTTTC TGGGGCCTCC ACCGGTGTCT CGGGCCTGGA TTCCACGGCC 
ATGGCCTCTG CCGCTGCGGC GCAGGGACTG TCCGGGGCGT CCGCGGCCAC CCTGCCCTTC 
CACCTCCAGC AGCACGTCCT GGCCTCTCAG GGCCTGGCCA TGTCCCCTTT CGGAAGCCTG 
TTCCCTTACC CCTACACGTA CATGGCCGCA GCGGCGGCCG CCTCCTCTGC GGCAGCCTCC 
AGCTCGGTGC ACCGCCACCC CTTCCTCAAT CTGAACACCA TGCGCCCGCG GCTGCGCTAC 
AGCCCCTACT CCATCCCGGT GCCGGTCCCG GACGGCAGCA GTCTGCTCAC CACCGCCCTG 
CCCTCCATGG CGGCGGCCGC GGGGCCCCTG GACGGCAAAG TCGCCGCCCT GGCCGCCAGC 
CCGGCCTCGG TGGCAGTGGA CTCGGGCTCT GAACTCAACA GCCGCTCCTC CACGCTCTCC 
TCCAGCTCCA TGTCCTTGTC GCCCAAACTC TGCGCGGAGA AAGAGGCGGC CACCAGCGAA 
CTGCAGAGCA TCCAGCGGTT GGTTAGCGGC TTGGAAGCCA AGCCGGACAG GTCCCGCAGC 
GCGTCCCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX3 ( NM_005996.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005987.3 Homo sapiens T-box transcription factor 3 (TBX3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17127
Clone ID Related Accession (Same CDS sequence)	NM_005996.3 , NM_005996.4
Accession Version	NM_005996.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Homo sapiens(human)
Product	T-box transcription factor TBX3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026765.22, AK054604.1, BC025258.1 and AF140240.1. On Nov 22, 2018 this sequence version replaced NM_005996.3. Transcript Variant: This variant (1) encodes the shorter isoform (1) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.82626.1, AF170708.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267762 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000349155.7/ ENSP00000257567.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCCTCT CCATGAGAGA TCCGGTCATT CCTGGGACAA GCATGGCCTA CCATCCGTTC 
CTACCTCACC GGGCGCCGGA CTTCGCCATG AGCGCGGTGC TGGGTCACCA GCCGCCGTTC 
TTCCCCGCGC TGACGCTGCC TCCCAACGGC GCGGCGGCGC TCTCGCTGCC GGGCGCCCTG 
GCCAAGCCGA TCATGGATCA ATTGGTGGGG GCGGCCGAGA CCGGCATCCC GTTCTCCTCC 
CTGGGGCCCC AGGCGCATCT GAGGCCTTTG AAGACCATGG AGCCCGAAGA AGAGGTGGAG 
GACGACCCCA AGGTGCACCT GGAGGCTAAA GAACTTTGGG ATCAGTTTCA CAAGCGGGGC 
ACCGAGATGG TCATTACCAA GTCGGGAAGG CGAATGTTTC CTCCATTTAA AGTGAGATGT 
TCTGGGCTGG ATAAAAAAGC CAAATACATT TTATTGATGG ACATTATAGC TGCTGATGAC 
TGTCGTTATA AATTTCACAA TTCTCGGTGG ATGGTGGCTG GTAAGGCCGA CCCCGAAATG 
CCAAAGAGGA TGTACATTCA CCCGGACAGC CCCGCTACTG GGGAACAGTG GATGTCCAAA 
GTCGTCACTT TCCACAAACT GAAACTCACC AACAACATTT CAGACAAACA TGGATTTACT 
ATATTGAACT CCATGCACAA ATACCAGCCC CGGTTCCACA TTGTAAGAGC CAATGACATC 
TTGAAACTCC CTTATAGTAC ATTTCGGACA TACTTGTTCC CCGAAACTGA ATTCATCGCT 
GTGACTGCAT ACCAGAATGA TAAGATAACC CAGTTAAAAA TAGACAACAA CCCTTTTGCA 
AAAGGTTTCC GGGACACTGG AAATGGCCGA AGAGAAAAAA GAAAACAGCT CACCCTGCAG 
TCCATGAGGG TGTTTGATGA AAGACACAAA AAGGAGAATG GGACCTCTGA TGAGTCCTCC 
AGTGAACAAG CAGCTTTCAA CTGCTTCGCC CAGGCTTCTT CTCCAGCCGC CTCCACTGTA 
GGGACATCGA ACCTCAAAGA TTTATGTCCC AGCGAGGGTG AGAGCGACGC CGAGGCCGAG 
AGCAAAGAGG AGCATGGCCC CGAGGCCTGC GACGCGGCCA AGATCTCCAC CACCACGTCG 
GAGGAGCCCT GCCGTGACAA GGGCAGCCCC GCGGTCAAGG CTCACCTTTT CGCTGCTGAG 
CGGCCCCGGG ACAGCGGGCG GCTGGACAAA GCGTCGCCCG ACTCACGCCA TAGCCCCGCC 
ACCATCTCGT CCAGCACTCG CGGCCTGGGC GCGGAGGAGC GCAGGAGCCC GGTTCGCGAG 
GGCACAGCGC CGGCCAAGGT GGAAGAGGCG CGCGCGCTCC CGGGCAAGGA GGCCTTCGCG 
CCGCTCACGG TGCAGACGGA CGCGGCCGCC GCGCACCTGG CCCAGGGCCC CCTGCCTGGC 
CTCGGCTTCG CCCCGGGCCT GGCGGGCCAA CAGTTCTTCA ACGGGCACCC GCTCTTCCTG 
CACCCCAGCC AGTTTGCCAT GGGGGGCGCC TTCTCCAGCA TGGCGGCCGC TGGCATGGGT 
CCCCTCCTGG CCACGGTTTC TGGGGCCTCC ACCGGTGTCT CGGGCCTGGA TTCCACGGCC 
ATGGCCTCTG CCGCTGCGGC GCAGGGACTG TCCGGGGCGT CCGCGGCCAC CCTGCCCTTC 
CACCTCCAGC AGCACGTCCT GGCCTCTCAG GGCCTGGCCA TGTCCCCTTT CGGAAGCCTG 
TTCCCTTACC CCTACACGTA CATGGCCGCA GCGGCGGCCG CCTCCTCTGC GGCAGCCTCC 
AGCTCGGTGC ACCGCCACCC CTTCCTCAAT CTGAACACCA TGCGCCCGCG GCTGCGCTAC 
AGCCCCTACT CCATCCCGGT GCCGGTCCCG GACGGCAGCA GTCTGCTCAC CACCGCCCTG 
CCCTCCATGG CGGCGGCCGC GGGGCCCCTG GACGGCAAAG TCGCCGCCCT GGCCGCCAGC 
CCGGCCTCGG TGGCAGTGGA CTCGGGCTCT GAACTCAACA GCCGCTCCTC CACGCTCTCC 
TCCAGCTCCA TGTCCTTGTC GCCCAAACTC TGCGCGGAGA AAGAGGCGGC CACCAGCGAA 
CTGCAGAGCA TCCAGCGGTT GGTTAGCGGC TTGGAAGCCA AGCCGGACAG GTCCCGCAGC 
GCGTCCCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005987.3
CDS	976..3147
Translation	MSLSMRDPVIPGTSMAYHPFLPHRAPDFAMSAVLGHQPPFFPALTLPPNGAAALSLPGALAKPIMDQLVGAAETGIPFSSLGPQAHLRPLKTMEPEEEVEDDPKVHLEAKELWDQFHKRGTEMVITKSGRRMFPPFKVRCSGLDKKAKYILLMDIIAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMSKVVTFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYLFPETEFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNGRREKRKQLTLQSMRVFDERHKKENGTSDESSSEQAAFNCFAQASSPAASTVGTSNLKDLCPSEGESDAEAESKEEHGPEACDAAKISTTTSEEPCRDKGSPAVKAHLFAAERPRDSGRLDKASPDSRHSPATISSSTRGLGAEERRSPVREGTAPAKVEEARALPGKEAFAPLTVQTDAAAAHLAQGPLPGLGFAPGLAGQQFFNGHPLFLHPSQFAMGGAFSSMAAAGMGPLLATVSGASTGVSGLDSTAMASAAAAQGLSGASAATLPFHLQQHVLASQGLAMSPFGSLFPYPYTYMAAAAAASSAAASSSVHRHPFLNLNTMRPRLRYSPYSIPVPVPDGSSLLTTALPSMAAAAGPLDGKVAALAASPASVAVDSGSELNSRSSTLSSSSMSLSPKLCAEKEAATSELQSIQRLVSGLEAKPDRSRSASP

Target ORF information:

RefSeq Version	NM_005996.4
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box transcription factor 3 (TBX3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005996.4

ORF Insert Sequence:

ATGAGCCTCT CCATGAGAGA TCCGGTCATT CCTGGGACAA GCATGGCCTA CCATCCGTTC 
CTACCTCACC GGGCGCCGGA CTTCGCCATG AGCGCGGTGC TGGGTCACCA GCCGCCGTTC 
TTCCCCGCGC TGACGCTGCC TCCCAACGGC GCGGCGGCGC TCTCGCTGCC GGGCGCCCTG 
GCCAAGCCGA TCATGGATCA ATTGGTGGGG GCGGCCGAGA CCGGCATCCC GTTCTCCTCC 
CTGGGGCCCC AGGCGCATCT GAGGCCTTTG AAGACCATGG AGCCCGAAGA AGAGGTGGAG 
GACGACCCCA AGGTGCACCT GGAGGCTAAA GAACTTTGGG ATCAGTTTCA CAAGCGGGGC 
ACCGAGATGG TCATTACCAA GTCGGGAAGG CGAATGTTTC CTCCATTTAA AGTGAGATGT 
TCTGGGCTGG ATAAAAAAGC CAAATACATT TTATTGATGG ACATTATAGC TGCTGATGAC 
TGTCGTTATA AATTTCACAA TTCTCGGTGG ATGGTGGCTG GTAAGGCCGA CCCCGAAATG 
CCAAAGAGGA TGTACATTCA CCCGGACAGC CCCGCTACTG GGGAACAGTG GATGTCCAAA 
GTCGTCACTT TCCACAAACT GAAACTCACC AACAACATTT CAGACAAACA TGGATTTACT 
ATATTGAACT CCATGCACAA ATACCAGCCC CGGTTCCACA TTGTAAGAGC CAATGACATC 
TTGAAACTCC CTTATAGTAC ATTTCGGACA TACTTGTTCC CCGAAACTGA ATTCATCGCT 
GTGACTGCAT ACCAGAATGA TAAGATAACC CAGTTAAAAA TAGACAACAA CCCTTTTGCA 
AAAGGTTTCC GGGACACTGG AAATGGCCGA AGAGAAAAAA GAAAACAGCT CACCCTGCAG 
TCCATGAGGG TGTTTGATGA AAGACACAAA AAGGAGAATG GGACCTCTGA TGAGTCCTCC 
AGTGAACAAG CAGCTTTCAA CTGCTTCGCC CAGGCTTCTT CTCCAGCCGC CTCCACTGTA 
GGGACATCGA ACCTCAAAGA TTTATGTCCC AGCGAGGGTG AGAGCGACGC CGAGGCCGAG 
AGCAAAGAGG AGCATGGCCC CGAGGCCTGC GACGCGGCCA AGATCTCCAC CACCACGTCG 
GAGGAGCCCT GCCGTGACAA GGGCAGCCCC GCGGTCAAGG CTCACCTTTT CGCTGCTGAG 
CGGCCCCGGG ACAGCGGGCG GCTGGACAAA GCGTCGCCCG ACTCACGCCA TAGCCCCGCC 
ACCATCTCGT CCAGCACTCG CGGCCTGGGC GCGGAGGAGC GCAGGAGCCC GGTTCGCGAG 
GGCACAGCGC CGGCCAAGGT GGAAGAGGCG CGCGCGCTCC CGGGCAAGGA GGCCTTCGCG 
CCGCTCACGG TGCAGACGGA CGCGGCCGCC GCGCACCTGG CCCAGGGCCC CCTGCCTGGC 
CTCGGCTTCG CCCCGGGCCT GGCGGGCCAA CAGTTCTTCA ACGGGCACCC GCTCTTCCTG 
CACCCCAGCC AGTTTGCCAT GGGGGGCGCC TTCTCCAGCA TGGCGGCCGC TGGCATGGGT 
CCCCTCCTGG CCACGGTTTC TGGGGCCTCC ACCGGTGTCT CGGGCCTGGA TTCCACGGCC 
ATGGCCTCTG CCGCTGCGGC GCAGGGACTG TCCGGGGCGT CCGCGGCCAC CCTGCCCTTC 
CACCTCCAGC AGCACGTCCT GGCCTCTCAG GGCCTGGCCA TGTCCCCTTT CGGAAGCCTG 
TTCCCTTACC CCTACACGTA CATGGCCGCA GCGGCGGCCG CCTCCTCTGC GGCAGCCTCC 
AGCTCGGTGC ACCGCCACCC CTTCCTCAAT CTGAACACCA TGCGCCCGCG GCTGCGCTAC 
AGCCCCTACT CCATCCCGGT GCCGGTCCCG GACGGCAGCA GTCTGCTCAC CACCGCCCTG 
CCCTCCATGG CGGCGGCCGC GGGGCCCCTG GACGGCAAAG TCGCCGCCCT GGCCGCCAGC 
CCGGCCTCGG TGGCAGTGGA CTCGGGCTCT GAACTCAACA GCCGCTCCTC CACGCTCTCC 
TCCAGCTCCA TGTCCTTGTC GCCCAAACTC TGCGCGGAGA AAGAGGCGGC CACCAGCGAA 
CTGCAGAGCA TCCAGCGGTT GGTTAGCGGC TTGGAAGCCA AGCCGGACAG GTCCCGCAGC 
GCGTCCCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX3 ( NM_016569.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057653.3 Homo sapiens T-box 3 (TBX3), transcript variant 2, mRNA.

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CloneID	OHu18626
Clone ID Related Accession (Same CDS sequence)	NM_016569.3 , NM_016569.4
Accession Version	NM_016569.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	T-box transcription factor TBX3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK054604.1, BC025258.1 and AF140240.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2004 this sequence version replaced NM_016569.2. Transcript Variant: This variant (2) contains an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini and is longer compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC025258.1, SRR1803611.30627.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTCT CCATGAGAGA TCCGGTCATT CCTGGGACAA GCATGGCCTA CCATCCGTTC 
CTACCTCACC GGGCGCCGGA CTTCGCCATG AGCGCGGTGC TGGGTCACCA GCCGCCGTTC 
TTCCCCGCGC TGACGCTGCC TCCCAACGGC GCGGCGGCGC TCTCGCTGCC GGGCGCCCTG 
GCCAAGCCGA TCATGGATCA ATTGGTGGGG GCGGCCGAGA CCGGCATCCC GTTCTCCTCC 
CTGGGGCCCC AGGCGCATCT GAGGCCTTTG AAGACCATGG AGCCCGAAGA AGAGGTGGAG 
GACGACCCCA AGGTGCACCT GGAGGCTAAA GAACTTTGGG ATCAGTTTCA CAAGCGGGGC 
ACCGAGATGG TCATTACCAA GTCGGGAAGG CGAATGTTTC CTCCATTTAA AGTGAGATGT 
TCTGGGCTGG ATAAAAAAGC CAAATACATT TTATTGATGG ACATTATAGC TGCTGATGAC 
TGTCGTTATA AATTTCACAA TTCTCGGTGG ATGGTGGCTG GTAAGGCCGA CCCCGAAATG 
CCAAAGAGGA TGTACATTCA CCCGGACAGC CCCGCTACTG GGGAACAGTG GATGTCCAAA 
GTCGTCACTT TCCACAAACT GAAACTCACC AACAACATTT CAGACAAACA TGGATTTACT 
TTGGCCTTCC CAAGTGATCA CGCTACGTGG CAGGGGAATT ATAGTTTTGG TACTCAGACT 
ATATTGAACT CCATGCACAA ATACCAGCCC CGGTTCCACA TTGTAAGAGC CAATGACATC 
TTGAAACTCC CTTATAGTAC ATTTCGGACA TACTTGTTCC CCGAAACTGA ATTCATCGCT 
GTGACTGCAT ACCAGAATGA TAAGATAACC CAGTTAAAAA TAGACAACAA CCCTTTTGCA 
AAAGGTTTCC GGGACACTGG AAATGGCCGA AGAGAAAAAA GAAAACAGCT CACCCTGCAG 
TCCATGAGGG TGTTTGATGA AAGACACAAA AAGGAGAATG GGACCTCTGA TGAGTCCTCC 
AGTGAACAAG CAGCTTTCAA CTGCTTCGCC CAGGCTTCTT CTCCAGCCGC CTCCACTGTA 
GGGACATCGA ACCTCAAAGA TTTATGTCCC AGCGAGGGTG AGAGCGACGC CGAGGCCGAG 
AGCAAAGAGG AGCATGGCCC CGAGGCCTGC GACGCGGCCA AGATCTCCAC CACCACGTCG 
GAGGAGCCCT GCCGTGACAA GGGCAGCCCC GCGGTCAAGG CTCACCTTTT CGCTGCTGAG 
CGGCCCCGGG ACAGCGGGCG GCTGGACAAA GCGTCGCCCG ACTCACGCCA TAGCCCCGCC 
ACCATCTCGT CCAGCACTCG CGGCCTGGGC GCGGAGGAGC GCAGGAGCCC GGTTCGCGAG 
GGCACAGCGC CGGCCAAGGT GGAAGAGGCG CGCGCGCTCC CGGGCAAGGA GGCCTTCGCG 
CCGCTCACGG TGCAGACGGA CGCGGCCGCC GCGCACCTGG CCCAGGGCCC CCTGCCTGGC 
CTCGGCTTCG CCCCGGGCCT GGCGGGCCAA CAGTTCTTCA ACGGGCACCC GCTCTTCCTG 
CACCCCAGCC AGTTTGCCAT GGGGGGCGCC TTCTCCAGCA TGGCGGCCGC TGGCATGGGT 
CCCCTCCTGG CCACGGTTTC TGGGGCCTCC ACCGGTGTCT CGGGCCTGGA TTCCACGGCC 
ATGGCCTCTG CCGCTGCGGC GCAGGGACTG TCCGGGGCGT CCGCGGCCAC CCTGCCCTTC 
CACCTCCAGC AGCACGTCCT GGCCTCTCAG GGCCTGGCCA TGTCCCCTTT CGGAAGCCTG 
TTCCCTTACC CCTACACGTA CATGGCCGCA GCGGCGGCCG CCTCCTCTGC GGCAGCCTCC 
AGCTCGGTGC ACCGCCACCC CTTCCTCAAT CTGAACACCA TGCGCCCGCG GCTGCGCTAC 
AGCCCCTACT CCATCCCGGT GCCGGTCCCG GACGGCAGCA GTCTGCTCAC CACCGCCCTG 
CCCTCCATGG CGGCGGCCGC GGGGCCCCTG GACGGCAAAG TCGCCGCCCT GGCCGCCAGC 
CCGGCCTCGG TGGCAGTGGA CTCGGGCTCT GAACTCAACA GCCGCTCCTC CACGCTCTCC 
TCCAGCTCCA TGTCCTTGTC GCCCAAACTC TGCGCGGAGA AAGAGGCGGC CACCAGCGAA 
CTGCAGAGCA TCCAGCGGTT GGTTAGCGGC TTGGAAGCCA AGCCGGACAG GTCCCGCAGC 
GCGTCCCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057653.3
CDS	965..3196
Translation	MSLSMRDPVIPGTSMAYHPFLPHRAPDFAMSAVLGHQPPFFPALTLPPNGAAALSLPGALAKPIMDQLVGAAETGIPFSSLGPQAHLRPLKTMEPEEEVEDDPKVHLEAKELWDQFHKRGTEMVITKSGRRMFPPFKVRCSGLDKKAKYILLMDIIAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMSKVVTFHKLKLTNNISDKHGFTLAFPSDHATWQGNYSFGTQTILNSMHKYQPRFHIVRANDILKLPYSTFRTYLFPETEFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNGRREKRKQLTLQSMRVFDERHKKENGTSDESSSEQAAFNCFAQASSPAASTVGTSNLKDLCPSEGESDAEAESKEEHGPEACDAAKISTTTSEEPCRDKGSPAVKAHLFAAERPRDSGRLDKASPDSRHSPATISSSTRGLGAEERRSPVREGTAPAKVEEARALPGKEAFAPLTVQTDAAAAHLAQGPLPGLGFAPGLAGQQFFNGHPLFLHPSQFAMGGAFSSMAAAGMGPLLATVSGASTGVSGLDSTAMASAAAAQGLSGASAATLPFHLQQHVLASQGLAMSPFGSLFPYPYTYMAAAAAASSAAASSSVHRHPFLNLNTMRPRLRYSPYSIPVPVPDGSSLLTTALPSMAAAAGPLDGKVAALAASPASVAVDSGSELNSRSSTLSSSSMSLSPKLCAEKEAATSELQSIQRLVSGLEAKPDRSRSASP

Target ORF information:

RefSeq Version	NM_016569.3
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box 3 (TBX3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016569.3

ORF Insert Sequence:

ATGAGCCTCT CCATGAGAGA TCCGGTCATT CCTGGGACAA GCATGGCCTA CCATCCGTTC 
CTACCTCACC GGGCGCCGGA CTTCGCCATG AGCGCGGTGC TGGGTCACCA GCCGCCGTTC 
TTCCCCGCGC TGACGCTGCC TCCCAACGGC GCGGCGGCGC TCTCGCTGCC GGGCGCCCTG 
GCCAAGCCGA TCATGGATCA ATTGGTGGGG GCGGCCGAGA CCGGCATCCC GTTCTCCTCC 
CTGGGGCCCC AGGCGCATCT GAGGCCTTTG AAGACCATGG AGCCCGAAGA AGAGGTGGAG 
GACGACCCCA AGGTGCACCT GGAGGCTAAA GAACTTTGGG ATCAGTTTCA CAAGCGGGGC 
ACCGAGATGG TCATTACCAA GTCGGGAAGG CGAATGTTTC CTCCATTTAA AGTGAGATGT 
TCTGGGCTGG ATAAAAAAGC CAAATACATT TTATTGATGG ACATTATAGC TGCTGATGAC 
TGTCGTTATA AATTTCACAA TTCTCGGTGG ATGGTGGCTG GTAAGGCCGA CCCCGAAATG 
CCAAAGAGGA TGTACATTCA CCCGGACAGC CCCGCTACTG GGGAACAGTG GATGTCCAAA 
GTCGTCACTT TCCACAAACT GAAACTCACC AACAACATTT CAGACAAACA TGGATTTACT 
TTGGCCTTCC CAAGTGATCA CGCTACGTGG CAGGGGAATT ATAGTTTTGG TACTCAGACT 
ATATTGAACT CCATGCACAA ATACCAGCCC CGGTTCCACA TTGTAAGAGC CAATGACATC 
TTGAAACTCC CTTATAGTAC ATTTCGGACA TACTTGTTCC CCGAAACTGA ATTCATCGCT 
GTGACTGCAT ACCAGAATGA TAAGATAACC CAGTTAAAAA TAGACAACAA CCCTTTTGCA 
AAAGGTTTCC GGGACACTGG AAATGGCCGA AGAGAAAAAA GAAAACAGCT CACCCTGCAG 
TCCATGAGGG TGTTTGATGA AAGACACAAA AAGGAGAATG GGACCTCTGA TGAGTCCTCC 
AGTGAACAAG CAGCTTTCAA CTGCTTCGCC CAGGCTTCTT CTCCAGCCGC CTCCACTGTA 
GGGACATCGA ACCTCAAAGA TTTATGTCCC AGCGAGGGTG AGAGCGACGC CGAGGCCGAG 
AGCAAAGAGG AGCATGGCCC CGAGGCCTGC GACGCGGCCA AGATCTCCAC CACCACGTCG 
GAGGAGCCCT GCCGTGACAA GGGCAGCCCC GCGGTCAAGG CTCACCTTTT CGCTGCTGAG 
CGGCCCCGGG ACAGCGGGCG GCTGGACAAA GCGTCGCCCG ACTCACGCCA TAGCCCCGCC 
ACCATCTCGT CCAGCACTCG CGGCCTGGGC GCGGAGGAGC GCAGGAGCCC GGTTCGCGAG 
GGCACAGCGC CGGCCAAGGT GGAAGAGGCG CGCGCGCTCC CGGGCAAGGA GGCCTTCGCG 
CCGCTCACGG TGCAGACGGA CGCGGCCGCC GCGCACCTGG CCCAGGGCCC CCTGCCTGGC 
CTCGGCTTCG CCCCGGGCCT GGCGGGCCAA CAGTTCTTCA ACGGGCACCC GCTCTTCCTG 
CACCCCAGCC AGTTTGCCAT GGGGGGCGCC TTCTCCAGCA TGGCGGCCGC TGGCATGGGT 
CCCCTCCTGG CCACGGTTTC TGGGGCCTCC ACCGGTGTCT CGGGCCTGGA TTCCACGGCC 
ATGGCCTCTG CCGCTGCGGC GCAGGGACTG TCCGGGGCGT CCGCGGCCAC CCTGCCCTTC 
CACCTCCAGC AGCACGTCCT GGCCTCTCAG GGCCTGGCCA TGTCCCCTTT CGGAAGCCTG 
TTCCCTTACC CCTACACGTA CATGGCCGCA GCGGCGGCCG CCTCCTCTGC GGCAGCCTCC 
AGCTCGGTGC ACCGCCACCC CTTCCTCAAT CTGAACACCA TGCGCCCGCG GCTGCGCTAC 
AGCCCCTACT CCATCCCGGT GCCGGTCCCG GACGGCAGCA GTCTGCTCAC CACCGCCCTG 
CCCTCCATGG CGGCGGCCGC GGGGCCCCTG GACGGCAAAG TCGCCGCCCT GGCCGCCAGC 
CCGGCCTCGG TGGCAGTGGA CTCGGGCTCT GAACTCAACA GCCGCTCCTC CACGCTCTCC 
TCCAGCTCCA TGTCCTTGTC GCCCAAACTC TGCGCGGAGA AAGAGGCGGC CACCAGCGAA 
CTGCAGAGCA TCCAGCGGTT GGTTAGCGGC TTGGAAGCCA AGCCGGACAG GTCCCGCAGC 
GCGTCCCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX3 ( NM_016569.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057653.3 Homo sapiens T-box transcription factor 3 (TBX3), transcript variant 2, mRNA.

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CloneID	OHu18626
Clone ID Related Accession (Same CDS sequence)	NM_016569.3 , NM_016569.4
Accession Version	NM_016569.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	T-box transcription factor TBX3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026765.22, AK054604.1, BC025258.1 and AF140240.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_016569.3. Transcript Variant: This variant (2) contains an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini and is longer compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC025258.1, SRR7346977.1720879.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTCT CCATGAGAGA TCCGGTCATT CCTGGGACAA GCATGGCCTA CCATCCGTTC 
CTACCTCACC GGGCGCCGGA CTTCGCCATG AGCGCGGTGC TGGGTCACCA GCCGCCGTTC 
TTCCCCGCGC TGACGCTGCC TCCCAACGGC GCGGCGGCGC TCTCGCTGCC GGGCGCCCTG 
GCCAAGCCGA TCATGGATCA ATTGGTGGGG GCGGCCGAGA CCGGCATCCC GTTCTCCTCC 
CTGGGGCCCC AGGCGCATCT GAGGCCTTTG AAGACCATGG AGCCCGAAGA AGAGGTGGAG 
GACGACCCCA AGGTGCACCT GGAGGCTAAA GAACTTTGGG ATCAGTTTCA CAAGCGGGGC 
ACCGAGATGG TCATTACCAA GTCGGGAAGG CGAATGTTTC CTCCATTTAA AGTGAGATGT 
TCTGGGCTGG ATAAAAAAGC CAAATACATT TTATTGATGG ACATTATAGC TGCTGATGAC 
TGTCGTTATA AATTTCACAA TTCTCGGTGG ATGGTGGCTG GTAAGGCCGA CCCCGAAATG 
CCAAAGAGGA TGTACATTCA CCCGGACAGC CCCGCTACTG GGGAACAGTG GATGTCCAAA 
GTCGTCACTT TCCACAAACT GAAACTCACC AACAACATTT CAGACAAACA TGGATTTACT 
TTGGCCTTCC CAAGTGATCA CGCTACGTGG CAGGGGAATT ATAGTTTTGG TACTCAGACT 
ATATTGAACT CCATGCACAA ATACCAGCCC CGGTTCCACA TTGTAAGAGC CAATGACATC 
TTGAAACTCC CTTATAGTAC ATTTCGGACA TACTTGTTCC CCGAAACTGA ATTCATCGCT 
GTGACTGCAT ACCAGAATGA TAAGATAACC CAGTTAAAAA TAGACAACAA CCCTTTTGCA 
AAAGGTTTCC GGGACACTGG AAATGGCCGA AGAGAAAAAA GAAAACAGCT CACCCTGCAG 
TCCATGAGGG TGTTTGATGA AAGACACAAA AAGGAGAATG GGACCTCTGA TGAGTCCTCC 
AGTGAACAAG CAGCTTTCAA CTGCTTCGCC CAGGCTTCTT CTCCAGCCGC CTCCACTGTA 
GGGACATCGA ACCTCAAAGA TTTATGTCCC AGCGAGGGTG AGAGCGACGC CGAGGCCGAG 
AGCAAAGAGG AGCATGGCCC CGAGGCCTGC GACGCGGCCA AGATCTCCAC CACCACGTCG 
GAGGAGCCCT GCCGTGACAA GGGCAGCCCC GCGGTCAAGG CTCACCTTTT CGCTGCTGAG 
CGGCCCCGGG ACAGCGGGCG GCTGGACAAA GCGTCGCCCG ACTCACGCCA TAGCCCCGCC 
ACCATCTCGT CCAGCACTCG CGGCCTGGGC GCGGAGGAGC GCAGGAGCCC GGTTCGCGAG 
GGCACAGCGC CGGCCAAGGT GGAAGAGGCG CGCGCGCTCC CGGGCAAGGA GGCCTTCGCG 
CCGCTCACGG TGCAGACGGA CGCGGCCGCC GCGCACCTGG CCCAGGGCCC CCTGCCTGGC 
CTCGGCTTCG CCCCGGGCCT GGCGGGCCAA CAGTTCTTCA ACGGGCACCC GCTCTTCCTG 
CACCCCAGCC AGTTTGCCAT GGGGGGCGCC TTCTCCAGCA TGGCGGCCGC TGGCATGGGT 
CCCCTCCTGG CCACGGTTTC TGGGGCCTCC ACCGGTGTCT CGGGCCTGGA TTCCACGGCC 
ATGGCCTCTG CCGCTGCGGC GCAGGGACTG TCCGGGGCGT CCGCGGCCAC CCTGCCCTTC 
CACCTCCAGC AGCACGTCCT GGCCTCTCAG GGCCTGGCCA TGTCCCCTTT CGGAAGCCTG 
TTCCCTTACC CCTACACGTA CATGGCCGCA GCGGCGGCCG CCTCCTCTGC GGCAGCCTCC 
AGCTCGGTGC ACCGCCACCC CTTCCTCAAT CTGAACACCA TGCGCCCGCG GCTGCGCTAC 
AGCCCCTACT CCATCCCGGT GCCGGTCCCG GACGGCAGCA GTCTGCTCAC CACCGCCCTG 
CCCTCCATGG CGGCGGCCGC GGGGCCCCTG GACGGCAAAG TCGCCGCCCT GGCCGCCAGC 
CCGGCCTCGG TGGCAGTGGA CTCGGGCTCT GAACTCAACA GCCGCTCCTC CACGCTCTCC 
TCCAGCTCCA TGTCCTTGTC GCCCAAACTC TGCGCGGAGA AAGAGGCGGC CACCAGCGAA 
CTGCAGAGCA TCCAGCGGTT GGTTAGCGGC TTGGAAGCCA AGCCGGACAG GTCCCGCAGC 
GCGTCCCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057653.3
CDS	976..3207
Translation	MSLSMRDPVIPGTSMAYHPFLPHRAPDFAMSAVLGHQPPFFPALTLPPNGAAALSLPGALAKPIMDQLVGAAETGIPFSSLGPQAHLRPLKTMEPEEEVEDDPKVHLEAKELWDQFHKRGTEMVITKSGRRMFPPFKVRCSGLDKKAKYILLMDIIAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMSKVVTFHKLKLTNNISDKHGFTLAFPSDHATWQGNYSFGTQTILNSMHKYQPRFHIVRANDILKLPYSTFRTYLFPETEFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNGRREKRKQLTLQSMRVFDERHKKENGTSDESSSEQAAFNCFAQASSPAASTVGTSNLKDLCPSEGESDAEAESKEEHGPEACDAAKISTTTSEEPCRDKGSPAVKAHLFAAERPRDSGRLDKASPDSRHSPATISSSTRGLGAEERRSPVREGTAPAKVEEARALPGKEAFAPLTVQTDAAAAHLAQGPLPGLGFAPGLAGQQFFNGHPLFLHPSQFAMGGAFSSMAAAGMGPLLATVSGASTGVSGLDSTAMASAAAAQGLSGASAATLPFHLQQHVLASQGLAMSPFGSLFPYPYTYMAAAAAASSAAASSSVHRHPFLNLNTMRPRLRYSPYSIPVPVPDGSSLLTTALPSMAAAAGPLDGKVAALAASPASVAVDSGSELNSRSSTLSSSSMSLSPKLCAEKEAATSELQSIQRLVSGLEAKPDRSRSASP

Target ORF information:

RefSeq Version	NM_016569.4
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box transcription factor 3 (TBX3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016569.4

ORF Insert Sequence:

ATGAGCCTCT CCATGAGAGA TCCGGTCATT CCTGGGACAA GCATGGCCTA CCATCCGTTC 
CTACCTCACC GGGCGCCGGA CTTCGCCATG AGCGCGGTGC TGGGTCACCA GCCGCCGTTC 
TTCCCCGCGC TGACGCTGCC TCCCAACGGC GCGGCGGCGC TCTCGCTGCC GGGCGCCCTG 
GCCAAGCCGA TCATGGATCA ATTGGTGGGG GCGGCCGAGA CCGGCATCCC GTTCTCCTCC 
CTGGGGCCCC AGGCGCATCT GAGGCCTTTG AAGACCATGG AGCCCGAAGA AGAGGTGGAG 
GACGACCCCA AGGTGCACCT GGAGGCTAAA GAACTTTGGG ATCAGTTTCA CAAGCGGGGC 
ACCGAGATGG TCATTACCAA GTCGGGAAGG CGAATGTTTC CTCCATTTAA AGTGAGATGT 
TCTGGGCTGG ATAAAAAAGC CAAATACATT TTATTGATGG ACATTATAGC TGCTGATGAC 
TGTCGTTATA AATTTCACAA TTCTCGGTGG ATGGTGGCTG GTAAGGCCGA CCCCGAAATG 
CCAAAGAGGA TGTACATTCA CCCGGACAGC CCCGCTACTG GGGAACAGTG GATGTCCAAA 
GTCGTCACTT TCCACAAACT GAAACTCACC AACAACATTT CAGACAAACA TGGATTTACT 
TTGGCCTTCC CAAGTGATCA CGCTACGTGG CAGGGGAATT ATAGTTTTGG TACTCAGACT 
ATATTGAACT CCATGCACAA ATACCAGCCC CGGTTCCACA TTGTAAGAGC CAATGACATC 
TTGAAACTCC CTTATAGTAC ATTTCGGACA TACTTGTTCC CCGAAACTGA ATTCATCGCT 
GTGACTGCAT ACCAGAATGA TAAGATAACC CAGTTAAAAA TAGACAACAA CCCTTTTGCA 
AAAGGTTTCC GGGACACTGG AAATGGCCGA AGAGAAAAAA GAAAACAGCT CACCCTGCAG 
TCCATGAGGG TGTTTGATGA AAGACACAAA AAGGAGAATG GGACCTCTGA TGAGTCCTCC 
AGTGAACAAG CAGCTTTCAA CTGCTTCGCC CAGGCTTCTT CTCCAGCCGC CTCCACTGTA 
GGGACATCGA ACCTCAAAGA TTTATGTCCC AGCGAGGGTG AGAGCGACGC CGAGGCCGAG 
AGCAAAGAGG AGCATGGCCC CGAGGCCTGC GACGCGGCCA AGATCTCCAC CACCACGTCG 
GAGGAGCCCT GCCGTGACAA GGGCAGCCCC GCGGTCAAGG CTCACCTTTT CGCTGCTGAG 
CGGCCCCGGG ACAGCGGGCG GCTGGACAAA GCGTCGCCCG ACTCACGCCA TAGCCCCGCC 
ACCATCTCGT CCAGCACTCG CGGCCTGGGC GCGGAGGAGC GCAGGAGCCC GGTTCGCGAG 
GGCACAGCGC CGGCCAAGGT GGAAGAGGCG CGCGCGCTCC CGGGCAAGGA GGCCTTCGCG 
CCGCTCACGG TGCAGACGGA CGCGGCCGCC GCGCACCTGG CCCAGGGCCC CCTGCCTGGC 
CTCGGCTTCG CCCCGGGCCT GGCGGGCCAA CAGTTCTTCA ACGGGCACCC GCTCTTCCTG 
CACCCCAGCC AGTTTGCCAT GGGGGGCGCC TTCTCCAGCA TGGCGGCCGC TGGCATGGGT 
CCCCTCCTGG CCACGGTTTC TGGGGCCTCC ACCGGTGTCT CGGGCCTGGA TTCCACGGCC 
ATGGCCTCTG CCGCTGCGGC GCAGGGACTG TCCGGGGCGT CCGCGGCCAC CCTGCCCTTC 
CACCTCCAGC AGCACGTCCT GGCCTCTCAG GGCCTGGCCA TGTCCCCTTT CGGAAGCCTG 
TTCCCTTACC CCTACACGTA CATGGCCGCA GCGGCGGCCG CCTCCTCTGC GGCAGCCTCC 
AGCTCGGTGC ACCGCCACCC CTTCCTCAAT CTGAACACCA TGCGCCCGCG GCTGCGCTAC 
AGCCCCTACT CCATCCCGGT GCCGGTCCCG GACGGCAGCA GTCTGCTCAC CACCGCCCTG 
CCCTCCATGG CGGCGGCCGC GGGGCCCCTG GACGGCAAAG TCGCCGCCCT GGCCGCCAGC 
CCGGCCTCGG TGGCAGTGGA CTCGGGCTCT GAACTCAACA GCCGCTCCTC CACGCTCTCC 
TCCAGCTCCA TGTCCTTGTC GCCCAAACTC TGCGCGGAGA AAGAGGCGGC CACCAGCGAA 
CTGCAGAGCA TCCAGCGGTT GGTTAGCGGC TTGGAAGCCA AGCCGGACAG GTCCCGCAGC 
GCGTCCCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

T-box transcription factor Tbx3 contributes to human hepatocellular carcinoma cell migration and invasion by repressing E-cadherin expression.
Oncology research(2018 Jan)
Feng X,Yao W,Zhang Z,Yuan F,Liang L,Zhou J,Liu S,Song J

Novel TBX3 mutation in a family of Cypriot ancestry with ulnar-mammary syndrome.
Clinical dysmorphology26(2)61-65(2017 Apr)
Tanteles GA,Nicolaou N,Syrimis A,Metaxa R,Nicolaou M,Christophidou-Anastasiadou V,Skordis N

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TBX3 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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