TBCE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TBCE
Entrez Gene ID	6905
Full Name	tubulin folding cofactor E
Synonyms	HRD,KCS,KCS1,PEAMO,pac2
General protein information	Preferred Names tubulin folding cofactor E Names tubulin-specific chaperone E hypoparathyroidism, growth and mental retardation, and dysmorphism
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.3
Summary	Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	604934
Disorder Html:	Kenny-Caffey syndrome, type 1, 244460 (3); Hypoparathyroidism-retardation-dysmorphism syndrome, 241410 (3); Encephalopathy, progressive, with amyotrophy and optic atrophy, 617207 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001287802.1	NP_001274731.1	tubulin-specific chaperone E isoform c
NM_001287801.2	NP_001274730.1	tubulin-specific chaperone E isoform b
NM_003193.4	NP_003184.1	tubulin-specific chaperone E isoform a
NM_001287801.1	NP_001274730.1	tubulin-specific chaperone E isoform b
NM_001079515.2	NP_001072983.1	tubulin-specific chaperone E isoform a
NM_003193.5	NP_003184.1	tubulin-specific chaperone E isoform a
NM_001079515.3	NP_001072983.1	tubulin-specific chaperone E isoform a

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	K07H8.1	NP_501395.1
Oryza sativa (rice)	Os05g0105300	NP_001054410.1
Mus musculus (house mouse)	Tbce	NP_848027.1
Xenopus tropicalis (tropical clawed frog)	tbce	NP_001016012.1
Homo sapiens (human)	TBCE	NP_001274730.1
Canis lupus familiaris (dog)	TBCE	XP_005618862.1
Bos taurus (cattle)	TBCE	NP_001033121.1
Arabidopsis thaliana (thale cress)	PFI	NP_565017.1
Pan troglodytes (chimpanzee)	TBCE	XP_003308872.1
Rattus norvegicus (Norway rat)	Tbce	NP_001012161.1
Danio rerio (zebrafish)	tbce	NP_001035078.2
Drosophila melanogaster (fruit fly)	tbce	NP_610197.2
Magnaporthe oryzae (rice blast fungus)	MGG_00378	XP_003718699.1
	TBCE	XP_001100813.1
Gallus gallus (chicken)	TBCE	XP_423980.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010770	XP_318537.3
Neurospora crassa	NCU09139	XP_958911.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression and localization of tubulin cofactors TBCD and TBCE in human gametes.
Jim?nez-Moreno V, Agirregoitia E
Zygote (Cambridge, England)25(3)304-312(2017 Jun)

Biallelic TBCD Mutations Cause Early-Onset Neurodegenerative Encephalopathy.
Miyake N, Fukai R, Ohba C, Chihara T, Miura M, Shimizu H, Kakita A, Imagawa E, Shiina M, Ogata K, Okuno-Yuguchi J, Fueki N, Ogiso Y, Suzumura H, Watabe Y, Imataka G, Leong HY, Fattal-Valevski A, Kramer U, Miyatake S, Kato M, Okamoto N, Sato Y, Mitsuhashi S, Nishino I, Kaneko N, Nishiyama A, Tamura T, Mizuguchi T, Nakashima M, Tanaka F, Saitsu H, Matsumoto N
American journal of human genetics99(4)950-961(2016 Oct)

TBCE Mutations Cause Early-Onset Progressive Encephalopathy with Distal Spinal Muscular Atrophy.
Sferra A, Baillat G, Rizza T, Barresi S, Flex E, Tasca G, D'Amico A, Bellacchio E, Ciolfi A, Caputo V, Cecchetti S, Torella A, Zanni G, Diodato D, Piermarini E, Niceta M, Coppola A, Tedeschi E, Martinelli D, Dionisi-Vici C, Nigro V, Dallapiccola B, Compagnucci C, Tartaglia M, Haase G, Bertini E
American journal of human genetics99(4)974-983(2016 Oct)

Sanjad-Sakati syndrome in a Tunisian child.
Kerkeni E, Sakka R, Sfar S, Bouaziz S, Ghedira N, Ben Ameur K, Ben Hmida H, Chioukh FZ, Gh?dira ES, Gribaa M, Monastiri K
Archives de pediatrie : organe officiel de la Societe francaise de pediatrie22(9)951-5(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Although loss of function of TBCE has been documented to impact multiple developmental processes, the present findings provide evidence that hypomorphic TBCE mutations primarily drive neurodegeneration
Title: TBCE Mutations Cause Early-Onset Progressive Encephalopathy with Distal Spinal Muscular Atrophy.

Sanjad-Sakati syndrome molecular pathology has been shown to be due to mutations in the TBCE gene on chromosome 1q42-q43.
Title: Sanjad-Sakati syndrome in a Tunisian child.

the role of the human TBCE and TBCB chaperones in alpha-tubulin-beta-tubulin dissociation, was investigated.
Title: The structure of the complex between ?-tubulin, TBCE and TBCB reveals a tubulin dimer dissociation mechanism.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Human variation in alcohol response is influenced by variation in neuronal signaling genes.

The following TBCE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBCE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20839	NM_001079515.3 Latest version!	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu20839	NM_003193.5 Latest version!	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu21654	NM_001287802.1 Latest version!	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu21745	NM_001287801.2 Latest version!	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu21745	NM_001287801.1	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu20839	NM_003193.4	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu20839	NM_001079515.2	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TBCE ( NM_003193.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003184.1 Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20839
Clone ID Related Accession (Same CDS sequence)	NM_003193.4 , NM_001079515.2 , NM_003193.5 , NM_001079515.3
Accession Version	NM_003193.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	tubulin-specific chaperone E isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB110473.1, U61232.2 and BF064027.1. On Dec 20, 2013 this sequence version replaced NM_003193.3. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.203978.1, SRR1660803.116772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GTGCTGCGGT GTGTCGCGGG GTGCCCAGGC CTGGAGGAAC TCTACCTTGA GTCTAACAAC 
ATTTTCATTT CCGAAAGGCC AACAGATGTT CTCCAGACAG TCAAGTTATT AGATCTTTCC 
TCTAATCAAT TAATTGATGA AAATCAGCTG TATCTGATAG CCCACCTGCC CAGGTTAGAA 
CAATTAATCC TCTCTGACAC TGGAATTTCT TCTCTACATT TTCCGGATGC TGGAATTGGG 
TGCAAAACGT CCATGTTCCC ATCCTTGAAG TACCTGGTAG TAAACGACAA TCAGATATCA 
CAATGGTCGT TTTTCAATGA GCTAGAGAAG TTACCAAGTC TACGGGCTTT GTCCTGCCTA 
AGAAACCCCC TGACCAAAGA GGACAAAGAA GCAGAGACGG CGCGACTACT CATTATCGCC 
AGCATTGGCC AGCTGAAGAC GCTGAACAAA TGTGAGATTC TCCCCGAGGA GAGGCGGAGA 
GCTGAGCTTG ACTACCGAAA AGCTTTTGGA AATGAGTGGA AACAGGCTGG TGGACATAAG 
GATCCGGAAA AAAACAGACT CAGCGAAGAA TTCCTCACAG CCCATCCCAG ATACCAGTTC 
CTCTGCCTGA AATATGGTGC ACCTGAAGAT TGGGAACTCA AAACACAGCA ACCACTTATG 
CTGAAAAACC AGCTACTAAC ACTGAAGATA AAATACCCTC ATCAACTTGA TCAGAAAGTC 
CTGGAGAAAC AACTGCCGGG CTCCATGACA ATTCAAAAGG TGAAGGGATT GCTGTCACGT 
CTTCTCAAAG TTCCTGTGTC AGACCTTCTG TTGTCCTATG AAAGTCCCAA AAAGCCGGGC 
AGAGAAATCG AGCTGGAAAA TGACCTAAAG TCATTACAGT TTTATTCTGT GGAAAATGGA 
GATTGTCTAT TAGTGCGATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003184.1
CDS	177..1760
Translation	MSDTLTADVIGRRVEVNGEHATVRFAGVVPPVAGPWLGVEWDNPERGKHDGSHEGTVYFKCRHPTGGSFIRPNKVNFGTDFLTAIKNRYVLEDGPEEDRKEQIVTIGNKPVETIGFDSIMKQQSQLSKLQEVSLRNCAVSCAGEKGGVAEACPNIRKVDLSKNLLSSWDEVIHIADQLRHLEVLNVSENKLKFPSGSVLTGTLSVLKVLVLNQTGITWAEVLRCVAGCPGLEELYLESNNIFISERPTDVLQTVKLLDLSSNQLIDENQLYLIAHLPRLEQLILSDTGISSLHFPDAGIGCKTSMFPSLKYLVVNDNQISQWSFFNELEKLPSLRALSCLRNPLTKEDKEAETARLLIIASIGQLKTLNKCEILPEERRRAELDYRKAFGNEWKQAGGHKDPEKNRLSEEFLTAHPRYQFLCLKYGAPEDWELKTQQPLMLKNQLLTLKIKYPHQLDQKVLEKQLPGSMTIQKVKGLLSRLLKVPVSDLLLSYESPKKPGREIELENDLKSLQFYSVENGDCLLVRW

Target ORF information:

RefSeq Version	NM_003193.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003193.4

ORF Insert Sequence:

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GTGCTGCGGT GTGTCGCGGG GTGCCCAGGC CTGGAGGAAC TCTACCTTGA GTCTAACAAC 
ATTTTCATTT CCGAAAGGCC AACAGATGTT CTCCAGACAG TCAAGTTATT AGATCTTTCC 
TCTAATCAAT TAATTGATGA AAATCAGCTG TATCTGATAG CCCACCTGCC CAGGTTAGAA 
CAATTAATCC TCTCTGACAC TGGAATTTCT TCTCTACATT TTCCGGATGC TGGAATTGGG 
TGCAAAACGT CCATGTTCCC ATCCTTGAAG TACCTGGTAG TAAACGACAA TCAGATATCA 
CAATGGTCGT TTTTCAATGA GCTAGAGAAG TTACCAAGTC TACGGGCTTT GTCCTGCCTA 
AGAAACCCCC TGACCAAAGA GGACAAAGAA GCAGAGACGG CGCGACTACT CATTATCGCC 
AGCATTGGCC AGCTGAAGAC GCTGAACAAA TGTGAGATTC TCCCCGAGGA GAGGCGGAGA 
GCTGAGCTTG ACTACCGAAA AGCTTTTGGA AATGAGTGGA AACAGGCTGG TGGACATAAG 
GATCCGGAAA AAAACAGACT CAGCGAAGAA TTCCTCACAG CCCATCCCAG ATACCAGTTC 
CTCTGCCTGA AATATGGTGC ACCTGAAGAT TGGGAACTCA AAACACAGCA ACCACTTATG 
CTGAAAAACC AGCTACTAAC ACTGAAGATA AAATACCCTC ATCAACTTGA TCAGAAAGTC 
CTGGAGAAAC AACTGCCGGG CTCCATGACA ATTCAAAAGG TGAAGGGATT GCTGTCACGT 
CTTCTCAAAG TTCCTGTGTC AGACCTTCTG TTGTCCTATG AAAGTCCCAA AAAGCCGGGC 
AGAGAAATCG AGCTGGAAAA TGACCTAAAG TCATTACAGT TTTATTCTGT GGAAAATGGA 
GATTGTCTAT TAGTGCGATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBCE ( NM_001079515.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001072983.1 Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20839
Clone ID Related Accession (Same CDS sequence)	NM_003193.4 , NM_001079515.2 , NM_003193.5 , NM_001079515.3
Accession Version	NM_001079515.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	tubulin-specific chaperone E isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB110473.1, DA981183.1, U61232.2 and BF064027.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2013 this sequence version replaced NM_001079515.1. Transcript Variant: This variant (1) encodes isoform (a). Both variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.220914.1, SRR1660807.66528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GTGCTGCGGT GTGTCGCGGG GTGCCCAGGC CTGGAGGAAC TCTACCTTGA GTCTAACAAC 
ATTTTCATTT CCGAAAGGCC AACAGATGTT CTCCAGACAG TCAAGTTATT AGATCTTTCC 
TCTAATCAAT TAATTGATGA AAATCAGCTG TATCTGATAG CCCACCTGCC CAGGTTAGAA 
CAATTAATCC TCTCTGACAC TGGAATTTCT TCTCTACATT TTCCGGATGC TGGAATTGGG 
TGCAAAACGT CCATGTTCCC ATCCTTGAAG TACCTGGTAG TAAACGACAA TCAGATATCA 
CAATGGTCGT TTTTCAATGA GCTAGAGAAG TTACCAAGTC TACGGGCTTT GTCCTGCCTA 
AGAAACCCCC TGACCAAAGA GGACAAAGAA GCAGAGACGG CGCGACTACT CATTATCGCC 
AGCATTGGCC AGCTGAAGAC GCTGAACAAA TGTGAGATTC TCCCCGAGGA GAGGCGGAGA 
GCTGAGCTTG ACTACCGAAA AGCTTTTGGA AATGAGTGGA AACAGGCTGG TGGACATAAG 
GATCCGGAAA AAAACAGACT CAGCGAAGAA TTCCTCACAG CCCATCCCAG ATACCAGTTC 
CTCTGCCTGA AATATGGTGC ACCTGAAGAT TGGGAACTCA AAACACAGCA ACCACTTATG 
CTGAAAAACC AGCTACTAAC ACTGAAGATA AAATACCCTC ATCAACTTGA TCAGAAAGTC 
CTGGAGAAAC AACTGCCGGG CTCCATGACA ATTCAAAAGG TGAAGGGATT GCTGTCACGT 
CTTCTCAAAG TTCCTGTGTC AGACCTTCTG TTGTCCTATG AAAGTCCCAA AAAGCCGGGC 
AGAGAAATCG AGCTGGAAAA TGACCTAAAG TCATTACAGT TTTATTCTGT GGAAAATGGA 
GATTGTCTAT TAGTGCGATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072983.1
CDS	225..1808
Translation	MSDTLTADVIGRRVEVNGEHATVRFAGVVPPVAGPWLGVEWDNPERGKHDGSHEGTVYFKCRHPTGGSFIRPNKVNFGTDFLTAIKNRYVLEDGPEEDRKEQIVTIGNKPVETIGFDSIMKQQSQLSKLQEVSLRNCAVSCAGEKGGVAEACPNIRKVDLSKNLLSSWDEVIHIADQLRHLEVLNVSENKLKFPSGSVLTGTLSVLKVLVLNQTGITWAEVLRCVAGCPGLEELYLESNNIFISERPTDVLQTVKLLDLSSNQLIDENQLYLIAHLPRLEQLILSDTGISSLHFPDAGIGCKTSMFPSLKYLVVNDNQISQWSFFNELEKLPSLRALSCLRNPLTKEDKEAETARLLIIASIGQLKTLNKCEILPEERRRAELDYRKAFGNEWKQAGGHKDPEKNRLSEEFLTAHPRYQFLCLKYGAPEDWELKTQQPLMLKNQLLTLKIKYPHQLDQKVLEKQLPGSMTIQKVKGLLSRLLKVPVSDLLLSYESPKKPGREIELENDLKSLQFYSVENGDCLLVRW

Target ORF information:

RefSeq Version	NM_001079515.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079515.2

ORF Insert Sequence:

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GTGCTGCGGT GTGTCGCGGG GTGCCCAGGC CTGGAGGAAC TCTACCTTGA GTCTAACAAC 
ATTTTCATTT CCGAAAGGCC AACAGATGTT CTCCAGACAG TCAAGTTATT AGATCTTTCC 
TCTAATCAAT TAATTGATGA AAATCAGCTG TATCTGATAG CCCACCTGCC CAGGTTAGAA 
CAATTAATCC TCTCTGACAC TGGAATTTCT TCTCTACATT TTCCGGATGC TGGAATTGGG 
TGCAAAACGT CCATGTTCCC ATCCTTGAAG TACCTGGTAG TAAACGACAA TCAGATATCA 
CAATGGTCGT TTTTCAATGA GCTAGAGAAG TTACCAAGTC TACGGGCTTT GTCCTGCCTA 
AGAAACCCCC TGACCAAAGA GGACAAAGAA GCAGAGACGG CGCGACTACT CATTATCGCC 
AGCATTGGCC AGCTGAAGAC GCTGAACAAA TGTGAGATTC TCCCCGAGGA GAGGCGGAGA 
GCTGAGCTTG ACTACCGAAA AGCTTTTGGA AATGAGTGGA AACAGGCTGG TGGACATAAG 
GATCCGGAAA AAAACAGACT CAGCGAAGAA TTCCTCACAG CCCATCCCAG ATACCAGTTC 
CTCTGCCTGA AATATGGTGC ACCTGAAGAT TGGGAACTCA AAACACAGCA ACCACTTATG 
CTGAAAAACC AGCTACTAAC ACTGAAGATA AAATACCCTC ATCAACTTGA TCAGAAAGTC 
CTGGAGAAAC AACTGCCGGG CTCCATGACA ATTCAAAAGG TGAAGGGATT GCTGTCACGT 
CTTCTCAAAG TTCCTGTGTC AGACCTTCTG TTGTCCTATG AAAGTCCCAA AAAGCCGGGC 
AGAGAAATCG AGCTGGAAAA TGACCTAAAG TCATTACAGT TTTATTCTGT GGAAAATGGA 
GATTGTCTAT TAGTGCGATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBCE ( NM_003193.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003184.1 Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20839
Clone ID Related Accession (Same CDS sequence)	NM_003193.4 , NM_001079515.2 , NM_003193.5 , NM_001079515.3
Accession Version	NM_003193.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	tubulin-specific chaperone E isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB110473.1, U61232.2, BF064027.1 and FO393422.1. On Nov 23, 2018 this sequence version replaced NM_003193.4. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.77120.1, SRR1803613.26946.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000642610.2/ ENSP00000494796.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GTGCTGCGGT GTGTCGCGGG GTGCCCAGGC CTGGAGGAAC TCTACCTTGA GTCTAACAAC 
ATTTTCATTT CCGAAAGGCC AACAGATGTT CTCCAGACAG TCAAGTTATT AGATCTTTCC 
TCTAATCAAT TAATTGATGA AAATCAGCTG TATCTGATAG CCCACCTGCC CAGGTTAGAA 
CAATTAATCC TCTCTGACAC TGGAATTTCT TCTCTACATT TTCCGGATGC TGGAATTGGG 
TGCAAAACGT CCATGTTCCC ATCCTTGAAG TACCTGGTAG TAAACGACAA TCAGATATCA 
CAATGGTCGT TTTTCAATGA GCTAGAGAAG TTACCAAGTC TACGGGCTTT GTCCTGCCTA 
AGAAACCCCC TGACCAAAGA GGACAAAGAA GCAGAGACGG CGCGACTACT CATTATCGCC 
AGCATTGGCC AGCTGAAGAC GCTGAACAAA TGTGAGATTC TCCCCGAGGA GAGGCGGAGA 
GCTGAGCTTG ACTACCGAAA AGCTTTTGGA AATGAGTGGA AACAGGCTGG TGGACATAAG 
GATCCGGAAA AAAACAGACT CAGCGAAGAA TTCCTCACAG CCCATCCCAG ATACCAGTTC 
CTCTGCCTGA AATATGGTGC ACCTGAAGAT TGGGAACTCA AAACACAGCA ACCACTTATG 
CTGAAAAACC AGCTACTAAC ACTGAAGATA AAATACCCTC ATCAACTTGA TCAGAAAGTC 
CTGGAGAAAC AACTGCCGGG CTCCATGACA ATTCAAAAGG TGAAGGGATT GCTGTCACGT 
CTTCTCAAAG TTCCTGTGTC AGACCTTCTG TTGTCCTATG AAAGTCCCAA AAAGCCGGGC 
AGAGAAATCG AGCTGGAAAA TGACCTAAAG TCATTACAGT TTTATTCTGT GGAAAATGGA 
GATTGTCTAT TAGTGCGATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003184.1
CDS	110..1693
Translation	MSDTLTADVIGRRVEVNGEHATVRFAGVVPPVAGPWLGVEWDNPERGKHDGSHEGTVYFKCRHPTGGSFIRPNKVNFGTDFLTAIKNRYVLEDGPEEDRKEQIVTIGNKPVETIGFDSIMKQQSQLSKLQEVSLRNCAVSCAGEKGGVAEACPNIRKVDLSKNLLSSWDEVIHIADQLRHLEVLNVSENKLKFPSGSVLTGTLSVLKVLVLNQTGITWAEVLRCVAGCPGLEELYLESNNIFISERPTDVLQTVKLLDLSSNQLIDENQLYLIAHLPRLEQLILSDTGISSLHFPDAGIGCKTSMFPSLKYLVVNDNQISQWSFFNELEKLPSLRALSCLRNPLTKEDKEAETARLLIIASIGQLKTLNKCEILPEERRRAELDYRKAFGNEWKQAGGHKDPEKNRLSEEFLTAHPRYQFLCLKYGAPEDWELKTQQPLMLKNQLLTLKIKYPHQLDQKVLEKQLPGSMTIQKVKGLLSRLLKVPVSDLLLSYESPKKPGREIELENDLKSLQFYSVENGDCLLVRW

Target ORF information:

RefSeq Version	NM_003193.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003193.5

ORF Insert Sequence:

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GTGCTGCGGT GTGTCGCGGG GTGCCCAGGC CTGGAGGAAC TCTACCTTGA GTCTAACAAC 
ATTTTCATTT CCGAAAGGCC AACAGATGTT CTCCAGACAG TCAAGTTATT AGATCTTTCC 
TCTAATCAAT TAATTGATGA AAATCAGCTG TATCTGATAG CCCACCTGCC CAGGTTAGAA 
CAATTAATCC TCTCTGACAC TGGAATTTCT TCTCTACATT TTCCGGATGC TGGAATTGGG 
TGCAAAACGT CCATGTTCCC ATCCTTGAAG TACCTGGTAG TAAACGACAA TCAGATATCA 
CAATGGTCGT TTTTCAATGA GCTAGAGAAG TTACCAAGTC TACGGGCTTT GTCCTGCCTA 
AGAAACCCCC TGACCAAAGA GGACAAAGAA GCAGAGACGG CGCGACTACT CATTATCGCC 
AGCATTGGCC AGCTGAAGAC GCTGAACAAA TGTGAGATTC TCCCCGAGGA GAGGCGGAGA 
GCTGAGCTTG ACTACCGAAA AGCTTTTGGA AATGAGTGGA AACAGGCTGG TGGACATAAG 
GATCCGGAAA AAAACAGACT CAGCGAAGAA TTCCTCACAG CCCATCCCAG ATACCAGTTC 
CTCTGCCTGA AATATGGTGC ACCTGAAGAT TGGGAACTCA AAACACAGCA ACCACTTATG 
CTGAAAAACC AGCTACTAAC ACTGAAGATA AAATACCCTC ATCAACTTGA TCAGAAAGTC 
CTGGAGAAAC AACTGCCGGG CTCCATGACA ATTCAAAAGG TGAAGGGATT GCTGTCACGT 
CTTCTCAAAG TTCCTGTGTC AGACCTTCTG TTGTCCTATG AAAGTCCCAA AAAGCCGGGC 
AGAGAAATCG AGCTGGAAAA TGACCTAAAG TCATTACAGT TTTATTCTGT GGAAAATGGA 
GATTGTCTAT TAGTGCGATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBCE ( NM_001079515.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001072983.1 Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 1, mRNA.

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CloneID	OHu20839
Clone ID Related Accession (Same CDS sequence)	NM_003193.4 , NM_001079515.2 , NM_003193.5 , NM_001079515.3
Accession Version	NM_001079515.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	tubulin-specific chaperone E isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB110473.1, DA981183.1, U61232.2, BF064027.1 and FO393422.1. On May 31, 2019 this sequence version replaced NM_001079515.2. Transcript Variant: This variant (1) encodes isoform (a). Both variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.219490.1, SRR1803617.220914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GTGCTGCGGT GTGTCGCGGG GTGCCCAGGC CTGGAGGAAC TCTACCTTGA GTCTAACAAC 
ATTTTCATTT CCGAAAGGCC AACAGATGTT CTCCAGACAG TCAAGTTATT AGATCTTTCC 
TCTAATCAAT TAATTGATGA AAATCAGCTG TATCTGATAG CCCACCTGCC CAGGTTAGAA 
CAATTAATCC TCTCTGACAC TGGAATTTCT TCTCTACATT TTCCGGATGC TGGAATTGGG 
TGCAAAACGT CCATGTTCCC ATCCTTGAAG TACCTGGTAG TAAACGACAA TCAGATATCA 
CAATGGTCGT TTTTCAATGA GCTAGAGAAG TTACCAAGTC TACGGGCTTT GTCCTGCCTA 
AGAAACCCCC TGACCAAAGA GGACAAAGAA GCAGAGACGG CGCGACTACT CATTATCGCC 
AGCATTGGCC AGCTGAAGAC GCTGAACAAA TGTGAGATTC TCCCCGAGGA GAGGCGGAGA 
GCTGAGCTTG ACTACCGAAA AGCTTTTGGA AATGAGTGGA AACAGGCTGG TGGACATAAG 
GATCCGGAAA AAAACAGACT CAGCGAAGAA TTCCTCACAG CCCATCCCAG ATACCAGTTC 
CTCTGCCTGA AATATGGTGC ACCTGAAGAT TGGGAACTCA AAACACAGCA ACCACTTATG 
CTGAAAAACC AGCTACTAAC ACTGAAGATA AAATACCCTC ATCAACTTGA TCAGAAAGTC 
CTGGAGAAAC AACTGCCGGG CTCCATGACA ATTCAAAAGG TGAAGGGATT GCTGTCACGT 
CTTCTCAAAG TTCCTGTGTC AGACCTTCTG TTGTCCTATG AAAGTCCCAA AAAGCCGGGC 
AGAGAAATCG AGCTGGAAAA TGACCTAAAG TCATTACAGT TTTATTCTGT GGAAAATGGA 
GATTGTCTAT TAGTGCGATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072983.1
CDS	158..1741
Translation	MSDTLTADVIGRRVEVNGEHATVRFAGVVPPVAGPWLGVEWDNPERGKHDGSHEGTVYFKCRHPTGGSFIRPNKVNFGTDFLTAIKNRYVLEDGPEEDRKEQIVTIGNKPVETIGFDSIMKQQSQLSKLQEVSLRNCAVSCAGEKGGVAEACPNIRKVDLSKNLLSSWDEVIHIADQLRHLEVLNVSENKLKFPSGSVLTGTLSVLKVLVLNQTGITWAEVLRCVAGCPGLEELYLESNNIFISERPTDVLQTVKLLDLSSNQLIDENQLYLIAHLPRLEQLILSDTGISSLHFPDAGIGCKTSMFPSLKYLVVNDNQISQWSFFNELEKLPSLRALSCLRNPLTKEDKEAETARLLIIASIGQLKTLNKCEILPEERRRAELDYRKAFGNEWKQAGGHKDPEKNRLSEEFLTAHPRYQFLCLKYGAPEDWELKTQQPLMLKNQLLTLKIKYPHQLDQKVLEKQLPGSMTIQKVKGLLSRLLKVPVSDLLLSYESPKKPGREIELENDLKSLQFYSVENGDCLLVRW

Target ORF information:

RefSeq Version	NM_001079515.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079515.3

ORF Insert Sequence:

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GTGCTGCGGT GTGTCGCGGG GTGCCCAGGC CTGGAGGAAC TCTACCTTGA GTCTAACAAC 
ATTTTCATTT CCGAAAGGCC AACAGATGTT CTCCAGACAG TCAAGTTATT AGATCTTTCC 
TCTAATCAAT TAATTGATGA AAATCAGCTG TATCTGATAG CCCACCTGCC CAGGTTAGAA 
CAATTAATCC TCTCTGACAC TGGAATTTCT TCTCTACATT TTCCGGATGC TGGAATTGGG 
TGCAAAACGT CCATGTTCCC ATCCTTGAAG TACCTGGTAG TAAACGACAA TCAGATATCA 
CAATGGTCGT TTTTCAATGA GCTAGAGAAG TTACCAAGTC TACGGGCTTT GTCCTGCCTA 
AGAAACCCCC TGACCAAAGA GGACAAAGAA GCAGAGACGG CGCGACTACT CATTATCGCC 
AGCATTGGCC AGCTGAAGAC GCTGAACAAA TGTGAGATTC TCCCCGAGGA GAGGCGGAGA 
GCTGAGCTTG ACTACCGAAA AGCTTTTGGA AATGAGTGGA AACAGGCTGG TGGACATAAG 
GATCCGGAAA AAAACAGACT CAGCGAAGAA TTCCTCACAG CCCATCCCAG ATACCAGTTC 
CTCTGCCTGA AATATGGTGC ACCTGAAGAT TGGGAACTCA AAACACAGCA ACCACTTATG 
CTGAAAAACC AGCTACTAAC ACTGAAGATA AAATACCCTC ATCAACTTGA TCAGAAAGTC 
CTGGAGAAAC AACTGCCGGG CTCCATGACA ATTCAAAAGG TGAAGGGATT GCTGTCACGT 
CTTCTCAAAG TTCCTGTGTC AGACCTTCTG TTGTCCTATG AAAGTCCCAA AAAGCCGGGC 
AGAGAAATCG AGCTGGAAAA TGACCTAAAG TCATTACAGT TTTATTCTGT GGAAAATGGA 
GATTGTCTAT TAGTGCGATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBCE ( NM_001287802.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274731.1 Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu21654
Clone ID Related Accession (Same CDS sequence)	NM_001287802.1
Accession Version	NM_001287802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	tubulin-specific chaperone E isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB110473.1, U61232.2, AK093397.1 and BF064027.1. On Dec 20, 2013 this sequence version replaced XM_005273238.1. Transcript Variant: This variant (4) differs in its 5' UTR, initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (c) has a shorter and distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093397.1, SRR1803613.48754.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATCCAT GTGAACTCTG TTTTTCATGC AGTCAGCTGA GCAAGTTGCA AGAAGTTTCT 
CTGAGGAACT GTGCAGTAAG TTGTGCTGGT GAAAAAGGAG GAGTTGCTGA AGCATGTCCT 
AATATCAGAA AGGTAGATTT GTCAAAAAAC CTGTTGTCAT CATGGGATGA AGTGATACAC 
ATTGCTGATC AGCTCAGACA CCTGGAAGTC CTTAATGTCA GTGAAAATAA ACTAAAATTT 
CCCTCCGGTT CAGTATTAAC TGGAACGCTT TCTGTACTGA AGGTTTTAGT CCTCAATCAA 
ACAGGAATAA CGTGGGCTGA GGTGCTGCGG TGTGTCGCGG GGTGCCCAGG CCTGGAGGAA 
CTCTACCTTG AGTCTAACAA CATTTTCATT TCCGAAAGGC CAACAGATGT TCTCCAGACA 
GTCAAGTTAT TAGATCTTTC CTCTAATCAA TTAATTGATG AAAATCAGCT GTATCTGATA 
GCCCACCTGC CCAGGTTAGA ACAATTAATC CTCTCTGACA CTGGAATTTC TTCTCTACAT 
TTTCCGGATG CTGGAATTGG GTGCAAAACG TCCATGTTCC CATCCTTGAA GTACCTGGTA 
GTAAACGACA ATCAGATATC ACAATGGTCG TTTTTCAATG AGCTAGAGAA GTTACCAAGT 
CTACGGGCTT TGTCCTGCCT AAGAAACCCC CTGACCAAAG AGGACAAAGA AGCAGAGACG 
GCGCGACTAC TCATTATCGC CAGCATTGGC CAGCTGAAGA CGCTGAACAA ATGTGAGATT 
CTCCCCGAGG AGAGGCGGAG AGCTGAGCTT GACTACCGAA AAGCTTTTGG AAATGAGTGG 
AAACAGGCTG GTGGACATAA GGATCCGGAA AAAAACAGAC TCAGCGAAGA ATTCCTCACA 
GCCCATCCCA GATACCAGTT CCTCTGCCTG AAATATGGTG CACCTGAAGA TTGGGAACTC 
AAAACACAGC AACCACTTAT GCTGAAAAAC CAGCTACTAA CACTGAAGAT AAAATACCCT 
CATCAACTTG ATCAGAAAGT CCTGGAGAAA CAACTGCCGG GCTCCATGAC AATTCAAAAG 
GTGAAGGGAT TGCTGTCACG TCTTCTCAAA GTTCCTGTGT CAGACCTTCT GTTGTCCTAT 
GAAAGTCCCA AAAAGCCGGG CAGAGAAATC GAGCTGGAAA ATGACCTAAA GTCATTACAG 
TTTTATTCTG TGGAAAATGG AGATTGTCTA TTAGTGCGAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274731.1
CDS	487..1731
Translation	MYPCELCFSCSQLSKLQEVSLRNCAVSCAGEKGGVAEACPNIRKVDLSKNLLSSWDEVIHIADQLRHLEVLNVSENKLKFPSGSVLTGTLSVLKVLVLNQTGITWAEVLRCVAGCPGLEELYLESNNIFISERPTDVLQTVKLLDLSSNQLIDENQLYLIAHLPRLEQLILSDTGISSLHFPDAGIGCKTSMFPSLKYLVVNDNQISQWSFFNELEKLPSLRALSCLRNPLTKEDKEAETARLLIIASIGQLKTLNKCEILPEERRRAELDYRKAFGNEWKQAGGHKDPEKNRLSEEFLTAHPRYQFLCLKYGAPEDWELKTQQPLMLKNQLLTLKIKYPHQLDQKVLEKQLPGSMTIQKVKGLLSRLLKVPVSDLLLSYESPKKPGREIELENDLKSLQFYSVENGDCLLVRW

Target ORF information:

RefSeq Version	NM_001287802.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287802.1

ORF Insert Sequence:

ATGTATCCAT GTGAACTCTG TTTTTCATGC AGTCAGCTGA GCAAGTTGCA AGAAGTTTCT 
CTGAGGAACT GTGCAGTAAG TTGTGCTGGT GAAAAAGGAG GAGTTGCTGA AGCATGTCCT 
AATATCAGAA AGGTAGATTT GTCAAAAAAC CTGTTGTCAT CATGGGATGA AGTGATACAC 
ATTGCTGATC AGCTCAGACA CCTGGAAGTC CTTAATGTCA GTGAAAATAA ACTAAAATTT 
CCCTCCGGTT CAGTATTAAC TGGAACGCTT TCTGTACTGA AGGTTTTAGT CCTCAATCAA 
ACAGGAATAA CGTGGGCTGA GGTGCTGCGG TGTGTCGCGG GGTGCCCAGG CCTGGAGGAA 
CTCTACCTTG AGTCTAACAA CATTTTCATT TCCGAAAGGC CAACAGATGT TCTCCAGACA 
GTCAAGTTAT TAGATCTTTC CTCTAATCAA TTAATTGATG AAAATCAGCT GTATCTGATA 
GCCCACCTGC CCAGGTTAGA ACAATTAATC CTCTCTGACA CTGGAATTTC TTCTCTACAT 
TTTCCGGATG CTGGAATTGG GTGCAAAACG TCCATGTTCC CATCCTTGAA GTACCTGGTA 
GTAAACGACA ATCAGATATC ACAATGGTCG TTTTTCAATG AGCTAGAGAA GTTACCAAGT 
CTACGGGCTT TGTCCTGCCT AAGAAACCCC CTGACCAAAG AGGACAAAGA AGCAGAGACG 
GCGCGACTAC TCATTATCGC CAGCATTGGC CAGCTGAAGA CGCTGAACAA ATGTGAGATT 
CTCCCCGAGG AGAGGCGGAG AGCTGAGCTT GACTACCGAA AAGCTTTTGG AAATGAGTGG 
AAACAGGCTG GTGGACATAA GGATCCGGAA AAAAACAGAC TCAGCGAAGA ATTCCTCACA 
GCCCATCCCA GATACCAGTT CCTCTGCCTG AAATATGGTG CACCTGAAGA TTGGGAACTC 
AAAACACAGC AACCACTTAT GCTGAAAAAC CAGCTACTAA CACTGAAGAT AAAATACCCT 
CATCAACTTG ATCAGAAAGT CCTGGAGAAA CAACTGCCGG GCTCCATGAC AATTCAAAAG 
GTGAAGGGAT TGCTGTCACG TCTTCTCAAA GTTCCTGTGT CAGACCTTCT GTTGTCCTAT 
GAAAGTCCCA AAAAGCCGGG CAGAGAAATC GAGCTGGAAA ATGACCTAAA GTCATTACAG 
TTTTATTCTG TGGAAAATGG AGATTGTCTA TTAGTGCGAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBCE ( NM_001287801.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274730.1 Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu21745
Clone ID Related Accession (Same CDS sequence)	NM_001287801.2 , NM_001287801.1
Accession Version	NM_001287801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	tubulin-specific chaperone E isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB110473.1, AK296185.1, BF064027.1 and FO393422.1. On May 31, 2019 this sequence version replaced NM_001287801.1. Transcript Variant: This variant (3) differs in the 5' UTR and contains an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (b) is longer than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04284274 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GCTCATGCGC AGTGTGGTGG CAGCAGGCAC GGTCTCGACA TGCAGAAAGA CGCCAGCAAG 
TTCGTGGATC TGTGCGTGCT GCAGAAATGC TCCACCAGCA ACTGCATCAT CAGTGCCAAG 
GACCACACAT CCATGCGGAT GAACGTGGCC AAGGTGCTGC GGTGTGTCGC GGGGTGCCCA 
GGCCTGGAGG AACTCTACCT TGAGTCTAAC AACATTTTCA TTTCCGAAAG GCCAACAGAT 
GTTCTCCAGA CAGTCAAGTT ATTAGATCTT TCCTCTAATC AATTAATTGA TGAAAATCAG 
CTGTATCTGA TAGCCCACCT GCCCAGGTTA GAACAATTAA TCCTCTCTGA CACTGGAATT 
TCTTCTCTAC ATTTTCCGGA TGCTGGAATT GGGTGCAAAA CGTCCATGTT CCCATCCTTG 
AAGTACCTGG TAGTAAACGA CAATCAGATA TCACAATGGT CGTTTTTCAA TGAGCTAGAG 
AAGTTACCAA GTCTACGGGC TTTGTCCTGC CTAAGAAACC CCCTGACCAA AGAGGACAAA 
GAAGCAGAGA CGGCGCGACT ACTCATTATC GCCAGCATTG GCCAGCTGAA GACGCTGAAC 
AAATGTGAGA TTCTCCCCGA GGAGAGGCGG AGAGCTGAGC TTGACTACCG AAAAGCTTTT 
GGAAATGAGT GGAAACAGGC TGGTGGACAT AAGGATCCGG AAAAAAACAG ACTCAGCGAA 
GAATTCCTCA CAGCCCATCC CAGATACCAG TTCCTCTGCC TGAAATATGG TGCACCTGAA 
GATTGGGAAC TCAAAACACA GCAACCACTT ATGCTGAAAA ACCAGCTACT AACACTGAAG 
ATAAAATACC CTCATCAACT TGATCAGAAA GTCCTGGAGA AACAACTGCC GGGCTCCATG 
ACAATTCAAA AGGTGAAGGG ATTGCTGTCA CGTCTTCTCA AAGTTCCTGT GTCAGACCTT 
CTGTTGTCCT ATGAAAGTCC CAAAAAGCCG GGCAGAGAAA TCGAGCTGGA AAATGACCTA 
AAGTCATTAC AGTTTTATTC TGTGGAAAAT GGAGATTGTC TATTAGTGCG ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274730.1
CDS	110..1846
Translation	MSDTLTADVIGRRVEVNGEHATVRFAGVVPPVAGPWLGVEWDNPERGKHDGSHEGTVYFKCRHPTGGSFIRPNKVNFGTDFLTAIKNRYVLEDGPEEDRKEQIVTIGNKPVETIGFDSIMKQQSQLSKLQEVSLRNCAVSCAGEKGGVAEACPNIRKVDLSKNLLSSWDEVIHIADQLRHLEVLNVSENKLKFPSGSVLTGTLSVLKVLVLNQTGITWAEAHAQCGGSRHGLDMQKDASKFVDLCVLQKCSTSNCIISAKDHTSMRMNVAKVLRCVAGCPGLEELYLESNNIFISERPTDVLQTVKLLDLSSNQLIDENQLYLIAHLPRLEQLILSDTGISSLHFPDAGIGCKTSMFPSLKYLVVNDNQISQWSFFNELEKLPSLRALSCLRNPLTKEDKEAETARLLIIASIGQLKTLNKCEILPEERRRAELDYRKAFGNEWKQAGGHKDPEKNRLSEEFLTAHPRYQFLCLKYGAPEDWELKTQQPLMLKNQLLTLKIKYPHQLDQKVLEKQLPGSMTIQKVKGLLSRLLKVPVSDLLLSYESPKKPGREIELENDLKSLQFYSVENGDCLLVRW

Target ORF information:

RefSeq Version	NM_001287801.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287801.2

ORF Insert Sequence:

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GCTCATGCGC AGTGTGGTGG CAGCAGGCAC GGTCTCGACA TGCAGAAAGA CGCCAGCAAG 
TTCGTGGATC TGTGCGTGCT GCAGAAATGC TCCACCAGCA ACTGCATCAT CAGTGCCAAG 
GACCACACAT CCATGCGGAT GAACGTGGCC AAGGTGCTGC GGTGTGTCGC GGGGTGCCCA 
GGCCTGGAGG AACTCTACCT TGAGTCTAAC AACATTTTCA TTTCCGAAAG GCCAACAGAT 
GTTCTCCAGA CAGTCAAGTT ATTAGATCTT TCCTCTAATC AATTAATTGA TGAAAATCAG 
CTGTATCTGA TAGCCCACCT GCCCAGGTTA GAACAATTAA TCCTCTCTGA CACTGGAATT 
TCTTCTCTAC ATTTTCCGGA TGCTGGAATT GGGTGCAAAA CGTCCATGTT CCCATCCTTG 
AAGTACCTGG TAGTAAACGA CAATCAGATA TCACAATGGT CGTTTTTCAA TGAGCTAGAG 
AAGTTACCAA GTCTACGGGC TTTGTCCTGC CTAAGAAACC CCCTGACCAA AGAGGACAAA 
GAAGCAGAGA CGGCGCGACT ACTCATTATC GCCAGCATTG GCCAGCTGAA GACGCTGAAC 
AAATGTGAGA TTCTCCCCGA GGAGAGGCGG AGAGCTGAGC TTGACTACCG AAAAGCTTTT 
GGAAATGAGT GGAAACAGGC TGGTGGACAT AAGGATCCGG AAAAAAACAG ACTCAGCGAA 
GAATTCCTCA CAGCCCATCC CAGATACCAG TTCCTCTGCC TGAAATATGG TGCACCTGAA 
GATTGGGAAC TCAAAACACA GCAACCACTT ATGCTGAAAA ACCAGCTACT AACACTGAAG 
ATAAAATACC CTCATCAACT TGATCAGAAA GTCCTGGAGA AACAACTGCC GGGCTCCATG 
ACAATTCAAA AGGTGAAGGG ATTGCTGTCA CGTCTTCTCA AAGTTCCTGT GTCAGACCTT 
CTGTTGTCCT ATGAAAGTCC CAAAAAGCCG GGCAGAGAAA TCGAGCTGGA AAATGACCTA 
AAGTCATTAC AGTTTTATTC TGTGGAAAAT GGAGATTGTC TATTAGTGCG ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBCE ( NM_001287801.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274730.1 Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 3, mRNA.

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CloneID	OHu21745
Clone ID Related Accession (Same CDS sequence)	NM_001287801.2 , NM_001287801.1
Accession Version	NM_001287801.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	tubulin-specific chaperone E isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB110473.1, AK296185.1 and BF064027.1. On Dec 20, 2013 this sequence version replaced XM_005273235.1. Transcript Variant: This variant (3) differs in the 5' UTR and contains an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (b) is longer than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04284274 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GCTCATGCGC AGTGTGGTGG CAGCAGGCAC GGTCTCGACA TGCAGAAAGA CGCCAGCAAG 
TTCGTGGATC TGTGCGTGCT GCAGAAATGC TCCACCAGCA ACTGCATCAT CAGTGCCAAG 
GACCACACAT CCATGCGGAT GAACGTGGCC AAGGTGCTGC GGTGTGTCGC GGGGTGCCCA 
GGCCTGGAGG AACTCTACCT TGAGTCTAAC AACATTTTCA TTTCCGAAAG GCCAACAGAT 
GTTCTCCAGA CAGTCAAGTT ATTAGATCTT TCCTCTAATC AATTAATTGA TGAAAATCAG 
CTGTATCTGA TAGCCCACCT GCCCAGGTTA GAACAATTAA TCCTCTCTGA CACTGGAATT 
TCTTCTCTAC ATTTTCCGGA TGCTGGAATT GGGTGCAAAA CGTCCATGTT CCCATCCTTG 
AAGTACCTGG TAGTAAACGA CAATCAGATA TCACAATGGT CGTTTTTCAA TGAGCTAGAG 
AAGTTACCAA GTCTACGGGC TTTGTCCTGC CTAAGAAACC CCCTGACCAA AGAGGACAAA 
GAAGCAGAGA CGGCGCGACT ACTCATTATC GCCAGCATTG GCCAGCTGAA GACGCTGAAC 
AAATGTGAGA TTCTCCCCGA GGAGAGGCGG AGAGCTGAGC TTGACTACCG AAAAGCTTTT 
GGAAATGAGT GGAAACAGGC TGGTGGACAT AAGGATCCGG AAAAAAACAG ACTCAGCGAA 
GAATTCCTCA CAGCCCATCC CAGATACCAG TTCCTCTGCC TGAAATATGG TGCACCTGAA 
GATTGGGAAC TCAAAACACA GCAACCACTT ATGCTGAAAA ACCAGCTACT AACACTGAAG 
ATAAAATACC CTCATCAACT TGATCAGAAA GTCCTGGAGA AACAACTGCC GGGCTCCATG 
ACAATTCAAA AGGTGAAGGG ATTGCTGTCA CGTCTTCTCA AAGTTCCTGT GTCAGACCTT 
CTGTTGTCCT ATGAAAGTCC CAAAAAGCCG GGCAGAGAAA TCGAGCTGGA AAATGACCTA 
AAGTCATTAC AGTTTTATTC TGTGGAAAAT GGAGATTGTC TATTAGTGCG ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274730.1
CDS	177..1913
Translation	MSDTLTADVIGRRVEVNGEHATVRFAGVVPPVAGPWLGVEWDNPERGKHDGSHEGTVYFKCRHPTGGSFIRPNKVNFGTDFLTAIKNRYVLEDGPEEDRKEQIVTIGNKPVETIGFDSIMKQQSQLSKLQEVSLRNCAVSCAGEKGGVAEACPNIRKVDLSKNLLSSWDEVIHIADQLRHLEVLNVSENKLKFPSGSVLTGTLSVLKVLVLNQTGITWAEAHAQCGGSRHGLDMQKDASKFVDLCVLQKCSTSNCIISAKDHTSMRMNVAKVLRCVAGCPGLEELYLESNNIFISERPTDVLQTVKLLDLSSNQLIDENQLYLIAHLPRLEQLILSDTGISSLHFPDAGIGCKTSMFPSLKYLVVNDNQISQWSFFNELEKLPSLRALSCLRNPLTKEDKEAETARLLIIASIGQLKTLNKCEILPEERRRAELDYRKAFGNEWKQAGGHKDPEKNRLSEEFLTAHPRYQFLCLKYGAPEDWELKTQQPLMLKNQLLTLKIKYPHQLDQKVLEKQLPGSMTIQKVKGLLSRLLKVPVSDLLLSYESPKKPGREIELENDLKSLQFYSVENGDCLLVRW

Target ORF information:

RefSeq Version	NM_001287801.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tubulin folding cofactor E (TBCE), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287801.1

ORF Insert Sequence:

ATGAGTGACA CTTTGACAGC GGATGTCATT GGTCGAAGAG TTGAAGTTAA TGGAGAACAT 
GCAACAGTAC GTTTTGCTGG TGTTGTCCCT CCCGTGGCAG GACCCTGGTT AGGAGTAGAA 
TGGGACAATC CCGAGAGAGG AAAGCATGAT GGGAGCCACG AAGGGACTGT GTATTTTAAA 
TGCAGGCACC CGACAGGAGG ATCCTTTATT CGTCCGAACA AGGTAAATTT TGGAACAGAC 
TTTCTTACTG CAATTAAGAA CCGCTATGTG TTAGAAGATG GACCAGAGGA AGATAGAAAA 
GAGCAAATTG TTACAATTGG AAATAAACCT GTGGAGACTA TCGGTTTTGA CTCTATTATG 
AAACAGCAAA GTCAGCTGAG CAAGTTGCAA GAAGTTTCTC TGAGGAACTG TGCAGTAAGT 
TGTGCTGGTG AAAAAGGAGG AGTTGCTGAA GCATGTCCTA ATATCAGAAA GGTAGATTTG 
TCAAAAAACC TGTTGTCATC ATGGGATGAA GTGATACACA TTGCTGATCA GCTCAGACAC 
CTGGAAGTCC TTAATGTCAG TGAAAATAAA CTAAAATTTC CCTCCGGTTC AGTATTAACT 
GGAACGCTTT CTGTACTGAA GGTTTTAGTC CTCAATCAAA CAGGAATAAC GTGGGCTGAG 
GCTCATGCGC AGTGTGGTGG CAGCAGGCAC GGTCTCGACA TGCAGAAAGA CGCCAGCAAG 
TTCGTGGATC TGTGCGTGCT GCAGAAATGC TCCACCAGCA ACTGCATCAT CAGTGCCAAG 
GACCACACAT CCATGCGGAT GAACGTGGCC AAGGTGCTGC GGTGTGTCGC GGGGTGCCCA 
GGCCTGGAGG AACTCTACCT TGAGTCTAAC AACATTTTCA TTTCCGAAAG GCCAACAGAT 
GTTCTCCAGA CAGTCAAGTT ATTAGATCTT TCCTCTAATC AATTAATTGA TGAAAATCAG 
CTGTATCTGA TAGCCCACCT GCCCAGGTTA GAACAATTAA TCCTCTCTGA CACTGGAATT 
TCTTCTCTAC ATTTTCCGGA TGCTGGAATT GGGTGCAAAA CGTCCATGTT CCCATCCTTG 
AAGTACCTGG TAGTAAACGA CAATCAGATA TCACAATGGT CGTTTTTCAA TGAGCTAGAG 
AAGTTACCAA GTCTACGGGC TTTGTCCTGC CTAAGAAACC CCCTGACCAA AGAGGACAAA 
GAAGCAGAGA CGGCGCGACT ACTCATTATC GCCAGCATTG GCCAGCTGAA GACGCTGAAC 
AAATGTGAGA TTCTCCCCGA GGAGAGGCGG AGAGCTGAGC TTGACTACCG AAAAGCTTTT 
GGAAATGAGT GGAAACAGGC TGGTGGACAT AAGGATCCGG AAAAAAACAG ACTCAGCGAA 
GAATTCCTCA CAGCCCATCC CAGATACCAG TTCCTCTGCC TGAAATATGG TGCACCTGAA 
GATTGGGAAC TCAAAACACA GCAACCACTT ATGCTGAAAA ACCAGCTACT AACACTGAAG 
ATAAAATACC CTCATCAACT TGATCAGAAA GTCCTGGAGA AACAACTGCC GGGCTCCATG 
ACAATTCAAA AGGTGAAGGG ATTGCTGTCA CGTCTTCTCA AAGTTCCTGT GTCAGACCTT 
CTGTTGTCCT ATGAAAGTCC CAAAAAGCCG GGCAGAGAAA TCGAGCTGGA AAATGACCTA 
AAGTCATTAC AGTTTTATTC TGTGGAAAAT GGAGATTGTC TATTAGTGCG ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression and localization of tubulin cofactors TBCD and TBCE in human gametes.
Zygote (Cambridge, England)25(3)304-312(2017 Jun)
Jim?nez-Moreno V,Agirregoitia E

Biallelic TBCD Mutations Cause Early-Onset Neurodegenerative Encephalopathy.
American journal of human genetics99(4)950-961(2016 Oct)
Miyake N,Fukai R,Ohba C,Chihara T,Miura M,Shimizu H,Kakita A,Imagawa E,Shiina M,Ogata K,Okuno-Yuguchi J,Fueki N,Ogiso Y,Suzumura H,Watabe Y,Imataka G,Leong HY,Fattal-Valevski A,Kramer U,Miyatake S,Kato M,Okamoto N,Sato Y,Mitsuhashi S,Nishino I,Kaneko N,Nishiyama A,Tamura T,Mizuguchi T,Nakashima M,Tanaka F,Saitsu H,Matsumoto N

TBCE Mutations Cause Early-Onset Progressive Encephalopathy with Distal Spinal Muscular Atrophy.
American journal of human genetics99(4)974-983(2016 Oct)
Sferra A,Baillat G,Rizza T,Barresi S,Flex E,Tasca G,D'Amico A,Bellacchio E,Ciolfi A,Caputo V,Cecchetti S,Torella A,Zanni G,Diodato D,Piermarini E,Niceta M,Coppola A,Tedeschi E,Martinelli D,Dionisi-Vici C,Nigro V,Dallapiccola B,Compagnucci C,Tartaglia M,Haase G,Bertini E

Sanjad-Sakati syndrome in a Tunisian child.
Archives de pediatrie : organe officiel de la Societe francaise de pediatrie22(9)951-5(2015 Sep)
Kerkeni E,Sakka R,Sfar S,Bouaziz S,Ghedira N,Ben Ameur K,Ben Hmida H,Chioukh FZ,Gh?dira ES,Gribaa M,Monastiri K

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TBCE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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