TAF10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TAF10
Entrez Gene ID	6881
Full Name	TATA-box binding protein associated factor 10
Synonyms	TAF2A,TAF2H,TAFII30
General protein information	Preferred Names TATA-box binding protein associated factor 10 Names transcription initiation factor TFIID subunit 10 STAF28 TAF(II)30 TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa TAFII-30 TATA box binding protein (TBP)-associated factor, RNA polymerase II, H, 30kD transcription initiation factor TFIID 30 kD subunit transcription initiation factor TFIID 30 kDa subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.4
Summary	Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. [provided by RefSeq, Jul 2008].
Disorder MIM:	600475

mRNA and Protein(s)

mRNA	Protein	Name
NM_006284.4	NP_006275.1	transcription initiation factor TFIID subunit 10
NM_006284.3	NP_006275.1	transcription initiation factor TFIID subunit 10

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TAF10	XP_003312946.1
	TAF10	XP_001109210.1
Canis lupus familiaris (dog)	TAF10	XP_542454.2
Oryza sativa (rice)	Os09g0431500	NP_001063238.1
Xenopus tropicalis (tropical clawed frog)	taf10	NP_001017279.1
Homo sapiens (human)	TAF10	NP_006275.1
Mus musculus (house mouse)	Taf10	NP_064408.2
Drosophila melanogaster (fruit fly)	Taf10	NP_477463.1
Arabidopsis thaliana (thale cress)	TAFII15	NP_849481.1
Bos taurus (cattle)	TAF10	NP_001092438.1
Rattus norvegicus (Norway rat)	Taf10	NP_001128207.1
Danio rerio (zebrafish)	taf10	NP_001038276.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008612	XP_314706.4

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

LOXL2 Oxidizes Methylated TAF10 and Controls TFIID-Dependent Genes during Neural Progenitor Differentiation.
Iturbide A, Pascual-Reguant L, Fargas L, Cebri? JP, Alsina B, Garc?a de Herreros A, Peir? S
Molecular cell58(5)755-66(2015 Jun)

Cytoplasmic TAF2-TAF8-TAF10 complex provides evidence for nuclear holo-TFIID assembly from preformed submodules.
Trowitzsch S, Viola C, Scheer E, Conic S, Chavant V, Fournier M, Papai G, Ebong IO, Schaffitzel C, Zou J, Haffke M, Rappsilber J, Robinson CV, Schultz P, Tora L, Berger I
Nature communications66011(2015 Jan)

The architecture of human general transcription factor TFIID core complex.
Bieniossek C, Papai G, Schaffitzel C, Garzoni F, Chaillet M, Scheer E, Papadopoulos P, Tora L, Schultz P, Berger I
Nature493(7434)699-702(2013 Jan)

The integrator complex recognizes a new double mark on the RNA polymerase II carboxyl-terminal domain.
Egloff S, Szczepaniak SA, Dienstbier M, Taylor A, Knight S, Murphy S
The Journal of biological chemistry285(27)20564-9(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lysine oxidation of the transcription factor TAF10 by LOXL2 is a controlled protein modification and demonstrates a role for protein oxidation in regulating pluripotency genes.
Title: LOXL2 Oxidizes Methylated TAF10 and Controls TFIID-Dependent Genes during Neural Progenitor Differentiation.

the TAF2-TAF8-TAF10 complex demonstrates that there is a stepwise assembly pathway of nuclear holo-TFIID, regulated by nuclear import of preformed cytoplasmic submodules
Title: Cytoplasmic TAF2-TAF8-TAF10 complex provides evidence for nuclear holo-TFIID assembly from preformed submodules.

The carboxyl-terminal domain of the largest subunit of RNA polymerase II (pol II) comprises multiple tandem repeats of a heptapeptide.
Title: The integrator complex recognizes a new double mark on the RNA polymerase II carboxyl-terminal domain.

CDKN1C negatively regulates RNA polymerase II C-terminal domain phosphorylation in an E2F1-dependent manner
Title: CDKN1C negatively regulates RNA polymerase II C-terminal domain phosphorylation in an E2F1-dependent manner.

TAFII30 was required for optimal P4 promoter activity and for the repressive association of estrogen receptor.
Title: Estrogen-induced and TAFII30-mediated gene repression by direct recruitment of the estrogen receptor and co-repressors to the core promoter and its reversal by tamoxifen.

The following TAF10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TAF10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00745	NM_006284.4 Latest version!	Homo sapiens TATA-box binding protein associated factor 10 (TAF10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu00745	NM_006284.3	Homo sapiens TATA-box binding protein associated factor 10 (TAF10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TAF10 ( NM_006284.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006275.1 Homo sapiens TATA-box binding protein associated factor 10 (TAF10), mRNA.

Price & Availability↑

CloneID	OHu00745
Clone ID Related Accession (Same CDS sequence)	NM_006284.4 , NM_006284.3
Accession Version	NM_006284.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	transcription initiation factor TFIID subunit 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC091564.12, BM990572.1 and KF510380.1. On Nov 23, 2018 this sequence version replaced NM_006284.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM762151.1, BQ688500.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000299424.9/ ENSP00000299424.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCTGCA GCGGCTCCGG CGCGGACCCC GAGGCGGCGC CGGCCTCCGC CGCCTCGGCC 
CCGGGCCCCG CGCCCCCGGT CTCGGCTCCC GCCGCGCTGC CCTCCAGCAC CGCCGCGGAG 
AACAAGGCCA GCCCCGCGGG GACAGCGGGG GGACCTGGGG CTGGAGCAGC TGCTGGGGGC 
ACGGGACCCT TGGCGGCGCG GGCCGGGGAG CCAGCTGAGC GGCGTGGGGC GGCTCCGGTG 
TCGGCGGGTG GCGCGGCGCC CCCGGAGGGG GCCATATCTA ACGGGGTTTA CGTACTGCCG 
AGCGCGGCCA ACGGAGACGT GAAGCCCGTG GTGTCCAGCA CGCCTTTGGT GGACTTCTTG 
ATGCAGCTGG AAGATTACAC GCCTACGATC CCAGATGCAG TGACTGGTTA CTACCTGAAC 
CGTGCTGGCT TTGAGGCCTC AGACCCACGC ATAATTCGGC TCATCTCCTT AGCTGCCCAG 
AAATTCATCT CAGATATTGC CAATGATGCC CTACAGCACT GCAAAATGAA GGGCACGGCC 
TCCGGCAGCT CCCGGAGCAA GAGCAAGGAC CGCAAGTACA CTCTAACCAT GGAGGACTTG 
ACCCCTGCCC TCAGCGAGTA TGGCATCAAT GTGAAGAAGC CGCACTACTT CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006275.1
CDS	28..684
Translation	MSCSGSGADPEAAPASAASAPGPAPPVSAPAALPSSTAAENKASPAGTAGGPGAGAAAGGTGPLAARAGEPAERRGAAPVSAGGAAPPEGAISNGVYVLPSAANGDVKPVVSSTPLVDFLMQLEDYTPTIPDAVTGYYLNRAGFEASDPRIIRLISLAAQKFISDIANDALQHCKMKGTASGSSRSKSKDRKYTLTMEDLTPALSEYGINVKKPHYFT

Target ORF information:

RefSeq Version	NM_006284.4
Organism	Homo sapiens(human)
Definition	Homo sapiens TATA-box binding protein associated factor 10 (TAF10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006284.4

ORF Insert Sequence:

ATGAGCTGCA GCGGCTCCGG CGCGGACCCC GAGGCGGCGC CGGCCTCCGC CGCCTCGGCC 
CCGGGCCCCG CGCCCCCGGT CTCGGCTCCC GCCGCGCTGC CCTCCAGCAC CGCCGCGGAG 
AACAAGGCCA GCCCCGCGGG GACAGCGGGG GGACCTGGGG CTGGAGCAGC TGCTGGGGGC 
ACGGGACCCT TGGCGGCGCG GGCCGGGGAG CCAGCTGAGC GGCGTGGGGC GGCTCCGGTG 
TCGGCGGGTG GCGCGGCGCC CCCGGAGGGG GCCATATCTA ACGGGGTTTA CGTACTGCCG 
AGCGCGGCCA ACGGAGACGT GAAGCCCGTG GTGTCCAGCA CGCCTTTGGT GGACTTCTTG 
ATGCAGCTGG AAGATTACAC GCCTACGATC CCAGATGCAG TGACTGGTTA CTACCTGAAC 
CGTGCTGGCT TTGAGGCCTC AGACCCACGC ATAATTCGGC TCATCTCCTT AGCTGCCCAG 
AAATTCATCT CAGATATTGC CAATGATGCC CTACAGCACT GCAAAATGAA GGGCACGGCC 
TCCGGCAGCT CCCGGAGCAA GAGCAAGGAC CGCAAGTACA CTCTAACCAT GGAGGACTTG 
ACCCCTGCCC TCAGCGAGTA TGGCATCAAT GTGAAGAAGC CGCACTACTT CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF10 ( NM_006284.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006275.1 Homo sapiens TATA-box binding protein associated factor 10 (TAF10), mRNA.

Price & Availability↑

CloneID	OHu00745
Clone ID Related Accession (Same CDS sequence)	NM_006284.4 , NM_006284.3
Accession Version	NM_006284.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	transcription initiation factor TFIID subunit 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC091564.12 and BM990572.1. On May 3, 2011 this sequence version replaced NM_006284.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL549071.3, BQ688500.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGCTGCA GCGGCTCCGG CGCGGACCCC GAGGCGGCGC CGGCCTCCGC CGCCTCGGCC 
CCGGGCCCCG CGCCCCCGGT CTCGGCTCCC GCCGCGCTGC CCTCCAGCAC CGCCGCGGAG 
AACAAGGCCA GCCCCGCGGG GACAGCGGGG GGACCTGGGG CTGGAGCAGC TGCTGGGGGC 
ACGGGACCCT TGGCGGCGCG GGCCGGGGAG CCAGCTGAGC GGCGTGGGGC GGCTCCGGTG 
TCGGCGGGTG GCGCGGCGCC CCCGGAGGGG GCCATATCTA ACGGGGTTTA CGTACTGCCG 
AGCGCGGCCA ACGGAGACGT GAAGCCCGTG GTGTCCAGCA CGCCTTTGGT GGACTTCTTG 
ATGCAGCTGG AAGATTACAC GCCTACGATC CCAGATGCAG TGACTGGTTA CTACCTGAAC 
CGTGCTGGCT TTGAGGCCTC AGACCCACGC ATAATTCGGC TCATCTCCTT AGCTGCCCAG 
AAATTCATCT CAGATATTGC CAATGATGCC CTACAGCACT GCAAAATGAA GGGCACGGCC 
TCCGGCAGCT CCCGGAGCAA GAGCAAGGAC CGCAAGTACA CTCTAACCAT GGAGGACTTG 
ACCCCTGCCC TCAGCGAGTA TGGCATCAAT GTGAAGAAGC CGCACTACTT CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006275.1
CDS	56..712
Translation	MSCSGSGADPEAAPASAASAPGPAPPVSAPAALPSSTAAENKASPAGTAGGPGAGAAAGGTGPLAARAGEPAERRGAAPVSAGGAAPPEGAISNGVYVLPSAANGDVKPVVSSTPLVDFLMQLEDYTPTIPDAVTGYYLNRAGFEASDPRIIRLISLAAQKFISDIANDALQHCKMKGTASGSSRSKSKDRKYTLTMEDLTPALSEYGINVKKPHYFT

Target ORF information:

RefSeq Version	NM_006284.3
Organism	Homo sapiens(human)
Definition	Homo sapiens TATA-box binding protein associated factor 10 (TAF10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006284.3

ORF Insert Sequence:

ATGAGCTGCA GCGGCTCCGG CGCGGACCCC GAGGCGGCGC CGGCCTCCGC CGCCTCGGCC 
CCGGGCCCCG CGCCCCCGGT CTCGGCTCCC GCCGCGCTGC CCTCCAGCAC CGCCGCGGAG 
AACAAGGCCA GCCCCGCGGG GACAGCGGGG GGACCTGGGG CTGGAGCAGC TGCTGGGGGC 
ACGGGACCCT TGGCGGCGCG GGCCGGGGAG CCAGCTGAGC GGCGTGGGGC GGCTCCGGTG 
TCGGCGGGTG GCGCGGCGCC CCCGGAGGGG GCCATATCTA ACGGGGTTTA CGTACTGCCG 
AGCGCGGCCA ACGGAGACGT GAAGCCCGTG GTGTCCAGCA CGCCTTTGGT GGACTTCTTG 
ATGCAGCTGG AAGATTACAC GCCTACGATC CCAGATGCAG TGACTGGTTA CTACCTGAAC 
CGTGCTGGCT TTGAGGCCTC AGACCCACGC ATAATTCGGC TCATCTCCTT AGCTGCCCAG 
AAATTCATCT CAGATATTGC CAATGATGCC CTACAGCACT GCAAAATGAA GGGCACGGCC 
TCCGGCAGCT CCCGGAGCAA GAGCAAGGAC CGCAAGTACA CTCTAACCAT GGAGGACTTG 
ACCCCTGCCC TCAGCGAGTA TGGCATCAAT GTGAAGAAGC CGCACTACTT CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

LOXL2 Oxidizes Methylated TAF10 and Controls TFIID-Dependent Genes during Neural Progenitor Differentiation.
Molecular cell58(5)755-66(2015 Jun)
Iturbide A,Pascual-Reguant L,Fargas L,Cebri? JP,Alsina B,Garc?a de Herreros A,Peir? S

Cytoplasmic TAF2-TAF8-TAF10 complex provides evidence for nuclear holo-TFIID assembly from preformed submodules.
Nature communications66011(2015 Jan)
Trowitzsch S,Viola C,Scheer E,Conic S,Chavant V,Fournier M,Papai G,Ebong IO,Schaffitzel C,Zou J,Haffke M,Rappsilber J,Robinson CV,Schultz P,Tora L,Berger I

The architecture of human general transcription factor TFIID core complex.
Nature493(7434)699-702(2013 Jan)
Bieniossek C,Papai G,Schaffitzel C,Garzoni F,Chaillet M,Scheer E,Papadopoulos P,Tora L,Schultz P,Berger I

The integrator complex recognizes a new double mark on the RNA polymerase II carboxyl-terminal domain.
The Journal of biological chemistry285(27)20564-9(2010 Jul)
Egloff S,Szczepaniak SA,Dienstbier M,Taylor A,Knight S,Murphy S

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TAF10 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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