BTC cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BTC
Entrez Gene ID	685
Full Name	betacellulin
General protein information	Preferred Names betacellulin Names probetacellulin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q13.3
Summary	This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients. [provided by RefSeq, Nov 2015].
Disorder MIM:	600345

mRNA and Protein(s)

mRNA	Protein	Name
XM_011532211.1	XP_011530513.1	probetacellulin isoform X1
NM_001729.4	NP_001720.1	probetacellulin isoform 1 preproprotein
NM_001729.3	NP_001720.1	probetacellulin isoform 1 preproprotein
XM_017008568.1	XP_016864057.1	probetacellulin isoform X2
NM_001729.2	NP_001720.1	probetacellulin precursor
NM_001316963.1	NP_001303892.1	probetacellulin isoform 2 precursor
NM_001316963.2	NP_001303892.1	probetacellulin isoform 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC482682	XP_005629452.1
Rattus norvegicus (Norway rat)	Btc	NP_071592.1
Danio rerio (zebrafish)	zgc:153253	NP_001154295.1
Homo sapiens (human)	BTC	NP_001720.1
Pan troglodytes (chimpanzee)	BTC	XP_517223.2
	BTC	XP_001101880.1
Mus musculus (house mouse)	Btc	NP_031594.1
Danio rerio (zebrafish)	btc	NP_001038229.2
Gallus gallus (chicken)	BTC	NP_001004769.1
Bos taurus (cattle)	BTC	NP_776321.2
Xenopus tropicalis (tropical clawed frog)	LOC100497610	XP_002941813.1

Gene Ontology
Bibliography

Related articles in PubMed

Betacellulin induces Slug-mediated down-regulation of E-cadherin and cell migration in ovarian cancer cells.
Zhao J, Klausen C, Qiu X, Cheng JC, Chang HM, Leung PC
Oncotarget7(20)28881-90(2016 May)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Regulatory mechanisms of betacellulin in CXCL8 production from lung cancer cells.
Shi L, Wang L, Wang B, Cretoiu SM, Wang Q, Wang X, Chen C
Journal of translational medicine1270(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results suggest that betacellulin induces ovarian cancer migration and Slug-dependent E-cadherin down-regulation
Title: Betacellulin induces Slug-mediated down-regulation of E-cadherin and cell migration in ovarian cancer cells.

CXCL8 production from lung cancer cells could be initiated by an autocrine mechanism or external sources of BTC.
Title: Regulatory mechanisms of betacellulin in CXCL8 production from lung cancer cells.

Data suggest that BTC (betacellulin), AREG (amphiregulin), and EREG (epiregulin) induced prostaglandin E2 production by induction of COX-2 (prostaglandin-endoperoxide synthase 2) through MAP kinase signaling in granulosa cells.
Title: EGF-like growth factors induce COX-2-derived PGE2 production through ERK1/2 in human granulosa cells.

BTC has properties of increasing retinal vascular leakage that could contribute to the development of diabetic retinopathy.
Title: Regulation of retinal vascular permeability by betacellulin.

Data suggest a novel receptor-independent role for betacellulin intracellular-domain fragment signaling due to its ability to inhibit cell growth in vitro.
Title: Sequential and gamma-secretase-dependent processing of the betacellulin precursor generates a palmitoylated intracellular-domain fragment that inhibits cell growth.

The following BTC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BTC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16655	NM_001729.4 Latest version!	Homo sapiens betacellulin (BTC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu16655	XM_011532211.1 Latest version!	Homo sapiens betacellulin (BTC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu39056	NM_001316963.2 Latest version!	Homo sapiens betacellulin (BTC), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu95382	XM_017008568.1 Latest version!	Homo sapiens betacellulin (BTC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu16655	NM_001729.3	Homo sapiens betacellulin (BTC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu16655	NM_001729.2	Homo sapiens betacellulin (BTC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu39056	NM_001316963.1	Homo sapiens betacellulin (BTC), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BTC ( NM_001729.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001720.1 Homo sapiens betacellulin (BTC), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16655
Clone ID Related Accession (Same CDS sequence)	XM_011532211.1 , NM_001729.4 , NM_001729.3 , NM_001729.2
Accession Version	NM_001729.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	probetacellulin isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB109441.1, S55606.1, AC116645.4 and BX114471.1. On Nov 22, 2018 this sequence version replaced NM_001729.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.237006.1, S55606.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000395743.8/ ENSP00000379092.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCTGTGATGA AGGCTACATT 
GGAGCAAGGT GTGAGAGAGT TGACTTGTTT TACCTAAGAG GAGACAGAGG ACAGATTCTG 
GTGATTTGTT TGATAGCAGT TATGGTAGTT TTTATTATTT TGGTCATCGG TGTCTGCACA 
TGCTGTCACC CTCTTCGGAA ACGTCGTAAA AGAAAGAAGA AAGAAGAAGA AATGGAAACT 
CTGGGTAAAG ATATAACTCC TATCAATGAA GATATTGAAG AGACAAATAT TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001720.1
CDS	199..735
Translation	MDRAARCSGASSLPLLLALALGLVILHCVVADGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVCDEGYIGARCERVDLFYLRGDRGQILVICLIAVMVVFIILVIGVCTCCHPLRKRRKRKKKEEEMETLGKDITPINEDIEETNIA

Target ORF information:

RefSeq Version	NM_001729.4
Organism	Homo sapiens(human)
Definition	Homo sapiens betacellulin (BTC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001729.4

ORF Insert Sequence:

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCTGTGATGA AGGCTACATT 
GGAGCAAGGT GTGAGAGAGT TGACTTGTTT TACCTAAGAG GAGACAGAGG ACAGATTCTG 
GTGATTTGTT TGATAGCAGT TATGGTAGTT TTTATTATTT TGGTCATCGG TGTCTGCACA 
TGCTGTCACC CTCTTCGGAA ACGTCGTAAA AGAAAGAAGA AAGAAGAAGA AATGGAAACT 
CTGGGTAAAG ATATAACTCC TATCAATGAA GATATTGAAG AGACAAATAT TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTC ( NM_001729.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001720.1 Homo sapiens betacellulin (BTC), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16655
Clone ID Related Accession (Same CDS sequence)	XM_011532211.1 , NM_001729.4 , NM_001729.3 , NM_001729.2
Accession Version	NM_001729.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	probetacellulin isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI758136.1, AB109441.1, S55606.1, AC116645.4 and BX114471.1. On Nov 5, 2015 this sequence version replaced NM_001729.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.237006.1, S55606.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCTGTGATGA AGGCTACATT 
GGAGCAAGGT GTGAGAGAGT TGACTTGTTT TACCTAAGAG GAGACAGAGG ACAGATTCTG 
GTGATTTGTT TGATAGCAGT TATGGTAGTT TTTATTATTT TGGTCATCGG TGTCTGCACA 
TGCTGTCACC CTCTTCGGAA ACGTCGTAAA AGAAAGAAGA AAGAAGAAGA AATGGAAACT 
CTGGGTAAAG ATATAACTCC TATCAATGAA GATATTGAAG AGACAAATAT TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001720.1
CDS	362..898
Translation	MDRAARCSGASSLPLLLALALGLVILHCVVADGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVCDEGYIGARCERVDLFYLRGDRGQILVICLIAVMVVFIILVIGVCTCCHPLRKRRKRKKKEEEMETLGKDITPINEDIEETNIA

Target ORF information:

RefSeq Version	NM_001729.3
Organism	Homo sapiens(human)
Definition	Homo sapiens betacellulin (BTC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001729.3

ORF Insert Sequence:

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCTGTGATGA AGGCTACATT 
GGAGCAAGGT GTGAGAGAGT TGACTTGTTT TACCTAAGAG GAGACAGAGG ACAGATTCTG 
GTGATTTGTT TGATAGCAGT TATGGTAGTT TTTATTATTT TGGTCATCGG TGTCTGCACA 
TGCTGTCACC CTCTTCGGAA ACGTCGTAAA AGAAAGAAGA AAGAAGAAGA AATGGAAACT 
CTGGGTAAAG ATATAACTCC TATCAATGAA GATATTGAAG AGACAAATAT TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTC ( NM_001729.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001720.1 Homo sapiens betacellulin (BTC), mRNA.

Price & Availability↑

CloneID	OHu16655
Clone ID Related Accession (Same CDS sequence)	XM_011532211.1 , NM_001729.4 , NM_001729.3 , NM_001729.2
Accession Version	NM_001729.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	probetacellulin precursor
Comment

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCTGTGATGA AGGCTACATT 
GGAGCAAGGT GTGAGAGAGT TGACTTGTTT TACCTAAGAG GAGACAGAGG ACAGATTCTG 
GTGATTTGTT TGATAGCAGT TATGGTAGTT TTTATTATTT TGGTCATCGG TGTCTGCACA 
TGCTGTCACC CTCTTCGGAA ACGTCGTAAA AGAAAGAAGA AAGAAGAAGA AATGGAAACT 
CTGGGTAAAG ATATAACTCC TATCAATGAA GATATTGAAG AGACAAATAT TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001720.1
Translation	MDRAARCSGASSLPLLLALALGLVILHCVVADGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVCDEGYIGARCERVDLFYLRGDRGQILVICLIAVMVVFIILVIGVCTCCHPLRKRRKRKKKEEEMETLGKDITPINEDIEETNIA

Target ORF information:

RefSeq Version	NM_001729.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens betacellulin (BTC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001729.2

ORF Insert Sequence:

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCTGTGATGA AGGCTACATT 
GGAGCAAGGT GTGAGAGAGT TGACTTGTTT TACCTAAGAG GAGACAGAGG ACAGATTCTG 
GTGATTTGTT TGATAGCAGT TATGGTAGTT TTTATTATTT TGGTCATCGG TGTCTGCACA 
TGCTGTCACC CTCTTCGGAA ACGTCGTAAA AGAAAGAAGA AAGAAGAAGA AATGGAAACT 
CTGGGTAAAG ATATAACTCC TATCAATGAA GATATTGAAG AGACAAATAT TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTC ( XM_011532211.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530513.1 Homo sapiens betacellulin (BTC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu16655
Clone ID Related Accession (Same CDS sequence)	XM_011532211.1 , NM_001729.4 , NM_001729.3 , NM_001729.2
Accession Version	XM_011532211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probetacellulin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCTGTGATGA AGGCTACATT 
GGAGCAAGGT GTGAGAGAGT TGACTTGTTT TACCTAAGAG GAGACAGAGG ACAGATTCTG 
GTGATTTGTT TGATAGCAGT TATGGTAGTT TTTATTATTT TGGTCATCGG TGTCTGCACA 
TGCTGTCACC CTCTTCGGAA ACGTCGTAAA AGAAAGAAGA AAGAAGAAGA AATGGAAACT 
CTGGGTAAAG ATATAACTCC TATCAATGAA GATATTGAAG AGACAAATAT TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530513.1
CDS	343..879
Translation	MDRAARCSGASSLPLLLALALGLVILHCVVADGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVCDEGYIGARCERVDLFYLRGDRGQILVICLIAVMVVFIILVIGVCTCCHPLRKRRKRKKKEEEMETLGKDITPINEDIEETNIA

Target ORF information:

RefSeq Version	XM_011532211.1
Organism	Homo sapiens(human)
Definition	Homo sapiens betacellulin (BTC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532211.1

ORF Insert Sequence:

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCTGTGATGA AGGCTACATT 
GGAGCAAGGT GTGAGAGAGT TGACTTGTTT TACCTAAGAG GAGACAGAGG ACAGATTCTG 
GTGATTTGTT TGATAGCAGT TATGGTAGTT TTTATTATTT TGGTCATCGG TGTCTGCACA 
TGCTGTCACC CTCTTCGGAA ACGTCGTAAA AGAAAGAAGA AAGAAGAAGA AATGGAAACT 
CTGGGTAAAG ATATAACTCC TATCAATGAA GATATTGAAG AGACAAATAT TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTC ( NM_001316963.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303892.1 Homo sapiens betacellulin (BTC), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu39056
Clone ID Related Accession (Same CDS sequence)	NM_001316963.1 , NM_001316963.2
Accession Version	NM_001316963.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	probetacellulin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI758136.1, AB109441.1, S55606.1, BI552049.1, AC116645.4 and BX114471.1. On Nov 5, 2015 this sequence version replaced XM_005263190.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2, also known as BTC-beta or BTC-delta4) lacks the transmembrane domain and a portion of the EGF domain compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.163007.1, SRR1660809.240737.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCCCTCTTCG GAAACGTCGT 
AAAAGAAAGA AGAAAGAAGA AGAAATGGAA ACTCTGGGTA AAGATATAAC TCCTATCAAT 
GAAGATATTG AAGAGACAAA TATTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303892.1
CDS	362..751
Translation	MDRAARCSGASSLPLLLALALGLVILHCVVADGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVPLRKRRKRKKKEEEMETLGKDITPINEDIEETNIA

Target ORF information:

RefSeq Version	NM_001316963.1
Organism	Homo sapiens(human)
Definition	Homo sapiens betacellulin (BTC), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316963.1

ORF Insert Sequence:

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCCCTCTTCG GAAACGTCGT 
AAAAGAAAGA AGAAAGAAGA AGAAATGGAA ACTCTGGGTA AAGATATAAC TCCTATCAAT 
GAAGATATTG AAGAGACAAA TATTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTC ( NM_001316963.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303892.1 Homo sapiens betacellulin (BTC), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu39056
Clone ID Related Accession (Same CDS sequence)	NM_001316963.1 , NM_001316963.2
Accession Version	NM_001316963.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	probetacellulin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB109441.1, S55606.1, BI552049.1, AC116645.4 and BX114471.1. On May 31, 2019 this sequence version replaced NM_001316963.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2, also known as BTC-beta or BTC-delta4) lacks the transmembrane domain and a portion of the EGF domain compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.163007.1, SRR1660809.240737.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCCCTCTTCG GAAACGTCGT 
AAAAGAAAGA AGAAAGAAGA AGAAATGGAA ACTCTGGGTA AAGATATAAC TCCTATCAAT 
GAAGATATTG AAGAGACAAA TATTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303892.1
CDS	199..588
Translation	MDRAARCSGASSLPLLLALALGLVILHCVVADGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVPLRKRRKRKKKEEEMETLGKDITPINEDIEETNIA

Target ORF information:

RefSeq Version	NM_001316963.2
Organism	Homo sapiens(human)
Definition	Homo sapiens betacellulin (BTC), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316963.2

ORF Insert Sequence:

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGCTACCAC CACACAATCA 
AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 
AGATGCCGCT TCGTGGTGGC CGAGCAGACG CCCTCCTGTG TCCCTCTTCG GAAACGTCGT 
AAAAGAAAGA AGAAAGAAGA AGAAATGGAA ACTCTGGGTA AAGATATAAC TCCTATCAAT 
GAAGATATTG AAGAGACAAA TATTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTC ( XM_017008568.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864057.1 Homo sapiens betacellulin (BTC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu95382
Clone ID Related Accession (Same CDS sequence)	XM_017008568.1
Accession Version	XM_017008568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probetacellulin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGGGGTTAT TTCTGACTTA 
AAAAAAAGAA AACAAAACAC AGACCAATTA AAAAAGTGGG GTGCAGTAGA GAAGAGCCTC 
CTGTATTCCC TGGAAACTTC TATGAACTGG GAGTATGATG ACTTGGTGCA AACTGCTCCT 
TTCCTAGCAC CCTCGGGAAG TTGTAACAGA GCCAGGAACA CACTGAGCAA GTCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864057.1
CDS	394..753
Translation	MDRAARCSGASSLPLLLALALGLVILHCVVADGNSTRSPETNGLLCGDPEENCAGVISDLKKRKQNTDQLKKWGAVEKSLLYSLETSMNWEYDDLVQTAPFLAPSGSCNRARNTLSKSS

Target ORF information:

RefSeq Version	XM_017008568.1
Organism	Homo sapiens(human)
Definition	Homo sapiens betacellulin (BTC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008568.1

ORF Insert Sequence:

ATGGACCGGG CCGCCCGGTG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 
CTGGGTCTAG TGATCCTTCA CTGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 
ACTAATGGCC TCCTCTGTGG AGACCCTGAG GAAAACTGTG CAGGGGTTAT TTCTGACTTA 
AAAAAAAGAA AACAAAACAC AGACCAATTA AAAAAGTGGG GTGCAGTAGA GAAGAGCCTC 
CTGTATTCCC TGGAAACTTC TATGAACTGG GAGTATGATG ACTTGGTGCA AACTGCTCCT 
TTCCTAGCAC CCTCGGGAAG TTGTAACAGA GCCAGGAACA CACTGAGCAA GTCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Betacellulin induces Slug-mediated down-regulation of E-cadherin and cell migration in ovarian cancer cells.
Oncotarget7(20)28881-90(2016 May)
Zhao J,Klausen C,Qiu X,Cheng JC,Chang HM,Leung PC

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Regulatory mechanisms of betacellulin in CXCL8 production from lung cancer cells.
Journal of translational medicine1270(2014 Mar)
Shi L,Wang L,Wang B,Cretoiu SM,Wang Q,Wang X,Chen C

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BTC cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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