VAMP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VAMP1
Entrez Gene ID	6843
Full Name	vesicle associated membrane protein 1
Synonyms	SPAX1,SYB1,VAMP-1
General protein information	Preferred Names vesicle associated membrane protein 1 Names vesicle-associated membrane protein 1 vesicle-associated membrane protein 1 (synaptobrevin 1)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.31
Summary	Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2014].
Disorder MIM:	185880
Disorder Html:	Spastic ataxia 1, autosomal dominant, 108600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297438.1	NP_001284367.1	vesicle-associated membrane protein 1 isoform 4
NM_014231.4	NP_055046.1	vesicle-associated membrane protein 1 isoform 1
NM_001297438.2	NP_001284367.1	vesicle-associated membrane protein 1 isoform 4
NM_199245.2	NP_954740.1	vesicle-associated membrane protein 1 isoform 2
NM_016830.3	NP_058439.1	vesicle-associated membrane protein 1 isoform 3
NM_016830.4	NP_058439.1	vesicle-associated membrane protein 1 isoform 3
NM_199245.3	NP_954740.1	vesicle-associated membrane protein 1 isoform 2
NM_014231.5	NP_055046.1	vesicle-associated membrane protein 1 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	VAMP1	XP_003313489.1
	VAMP1	XP_002798503.1
Gallus gallus (chicken)	VAMP1	NP_001034575.1
Danio rerio (zebrafish)	zgc:92912	NP_001002532.1
Mus musculus (house mouse)	Vamp1	NP_033522.1
Danio rerio (zebrafish)	vamp1	NP_998563.1
Drosophila melanogaster (fruit fly)	Syb	NP_724906.1
Homo sapiens (human)	VAMP1	NP_055046.1
Canis lupus familiaris (dog)	VAMP1	XP_005637307.1
Caenorhabditis elegans (roundworm)	snb-1	NP_504688.1
Xenopus tropicalis (tropical clawed frog)	vamp1	NP_001106401.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007721	XP_001687848.1

Gene Ontology
Bibliography

Related articles in PubMed

Homozygous mutations in VAMP1 cause a presynaptic congenital myasthenic syndrome.
Salpietro V, Lin W, Delle Vedove A, Storbeck M, Liu Y, Efthymiou S, Manole A, Wiethoff S, Ye Q, Saggar A, McElreavey K, Krishnakumar SS, , Pitt M, Bello OD, Rothman JE, Basel-Vanagaite L, Hubshman MW, Aharoni S, Manzur AY, Wirth B, Houlden H
Annals of neurology81(4)597-603(2017 04)

Genetically-controlled Vesicle-Associated Membrane Protein 1 expression may contribute to Alzheimer's pathophysiology and susceptibility.
Sevlever D, Zou F, Ma L, Carrasquillo S, Crump MG, Culley OJ, Hunter TA, Bisceglio GD, Younkin L, Allen M, Carrasquillo MM, Sando SB, Aasly JO, Dickson DW, Graff-Radford NR, Petersen RC, De?k F, , Belbin O
Molecular neurodegeneration1018(2015 Apr)

Widespread sequence variations in VAMP1 across vertebrates suggest a potential selective pressure from botulinum neurotoxins.
Peng L, Adler M, Demogines A, Borrell A, Liu H, Tao L, Tepp WH, Zhang SC, Johnson EA, Sawyer SL, Dong M
PLoS pathogens10(7)e1004177(2014 Jul)

Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration.
Manca P, Mameli O, Caria MA, Torrej?n-Escribano B, Blasi J
Neuroscience266102-15(2014 Apr)

VAMP1 mutation causes dominant hereditary spastic ataxia in Newfoundland families.
Bourassa CV, Meijer IA, Merner ND, Grewal KK, Stefanelli MG, Hodgkinson K, Ives EJ, Pryse-Phillips W, Jog M, Boycott K, Grimes DA, Goobie S, Leckey R, Dion PA, Rouleau GA
American journal of human genetics91(3)548-52(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

VAMP1 homozygous mutations causes of presynaptic congenital myasthenic syndrome.
Title: Homozygous mutations in VAMP1 cause a presynaptic congenital myasthenic syndrome.

Genetically regulated VAMP1 expression in the brain may modify both Alzheimer's disease risk and may contribute to Alzheimer's pathophysiology
Title: Genetically-controlled Vesicle-Associated Membrane Protein 1 expression may contribute to Alzheimer's pathophysiology and susceptibility.

residue 48 of VAMP1 varies frequently between M and I across seventeen closely related primate species, suggesting a potential selective pressure from members of BoNTs for resistance in vertebrates.
Title: Widespread sequence variations in VAMP1 across vertebrates suggest a potential selective pressure from botulinum neurotoxins.

This report identifies vesicle-associated membrane protein 1 (VAMP1), which encodes a critical protein for synaptic exocytosis, as the responsible gene fora dominantly inherited spastic ataxia
Title: VAMP1 mutation causes dominant hereditary spastic ataxia in Newfoundland families.

multiple domains outside the R-SNARE of tomosyn are critical to the efficacy of inhibition by tomosyn on exocytotic secretion
Title: Structural and functional analysis of tomosyn identifies domains important in exocytotic regulation.

The following VAMP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VAMP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28793	NM_014231.5 Latest version!	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu28794	NM_199245.3 Latest version!	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu28795	NM_016830.4 Latest version!	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu28796	NM_001297438.2 Latest version!	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu28796	NM_001297438.1	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu28795	NM_016830.3	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu28794	NM_199245.2	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu28793	NM_014231.4	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VAMP1 ( NM_014231.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055046.1 Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28793
Clone ID Related Accession (Same CDS sequence)	NM_014231.4 , NM_014231.5
Accession Version	NM_014231.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR749485.1, BC023286.1, BQ717992.1, AC005840.2, BF338798.1, BF341451.1, R83084.1 and AF060538.1. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2014 this sequence version replaced NM_014231.3. Transcript Variant: This variant (1), also known as VAMP-1A, encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.222451.1, SRR1803613.120441.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA TCTACTTTTT TACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055046.1
CDS	369..725
Translation	MSAPAQPPAEGTEGTAPGGGPPGPPPNMTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCKMMIMLGAICAIIVVVIVIYFFT

Target ORF information:

RefSeq Version	NM_014231.4
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014231.4

ORF Insert Sequence:

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA TCTACTTTTT TACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP1 ( NM_014231.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055046.1 Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28793
Clone ID Related Accession (Same CDS sequence)	NM_014231.4 , NM_014231.5
Accession Version	NM_014231.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005840.2. On Jun 2, 2019 this sequence version replaced NM_014231.4. Transcript Variant: This variant (1), also known as VAMP-1A, encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.5491.1, SRR1803613.57314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA TCTACTTTTT TACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055046.1
CDS	147..503
Translation	MSAPAQPPAEGTEGTAPGGGPPGPPPNMTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCKMMIMLGAICAIIVVVIVIYFFT

Target ORF information:

RefSeq Version	NM_014231.5
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014231.5

ORF Insert Sequence:

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA TCTACTTTTT TACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP1 ( NM_199245.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_954740.1 Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28794
Clone ID Related Accession (Same CDS sequence)	NM_199245.2 , NM_199245.3
Accession Version	NM_199245.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR749485.1, AC005840.2, BC023286.1, BI838752.1, AU279974.1, CB215628.1, BF338798.1, BF341451.1, R83084.1 and AF060538.1. On Jul 3, 2014 this sequence version replaced NM_199245.1. Transcript Variant: This variant (2), also known as VAMP-1D, has a different 3' structure, resulting in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749485.1, SRR1803616.219748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GTAAGTATCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954740.1
CDS	369..722
Translation	MSAPAQPPAEGTEGTAPGGGPPGPPPNMTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCKMMIMLGAICAIIVVVIVSKYR

Target ORF information:

RefSeq Version	NM_199245.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199245.2

ORF Insert Sequence:

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GTAAGTATCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP1 ( NM_199245.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_954740.1 Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28794
Clone ID Related Accession (Same CDS sequence)	NM_199245.2 , NM_199245.3
Accession Version	NM_199245.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005840.2. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_199245.2. Transcript Variant: This variant (2), also known as VAMP-1D, has a different 3' structure, resulting in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749485.1, SRR1660807.227786.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GTAAGTATCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954740.1
CDS	147..500
Translation	MSAPAQPPAEGTEGTAPGGGPPGPPPNMTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCKMMIMLGAICAIIVVVIVSKYR

Target ORF information:

RefSeq Version	NM_199245.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199245.3

ORF Insert Sequence:

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GTAAGTATCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP1 ( NM_016830.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_058439.1 Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28795
Clone ID Related Accession (Same CDS sequence)	NM_016830.3 , NM_016830.4
Accession Version	NM_016830.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR749485.1, BM837373.1, AF060538.1 and BC023286.1. On Jul 3, 2014 this sequence version replaced NM_016830.2. Transcript Variant: This variant (3), also known as VAMP-1B, contains an alternate 3' terminal exon, resulting in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 1. Isoforms 3 and 4 are the same length but differ in their C-termini. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.90750.1, SRR1163655.267688.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GGCGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058439.1
CDS	369..719
Translation	MSAPAQPPAEGTEGTAPGGGPPGPPPNMTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCKMMIMLGAICAIIVVVIVRRD

Target ORF information:

RefSeq Version	NM_016830.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016830.3

ORF Insert Sequence:

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GGCGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP1 ( NM_016830.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_058439.1 Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 3, mRNA.

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CloneID	OHu28795
Clone ID Related Accession (Same CDS sequence)	NM_016830.3 , NM_016830.4
Accession Version	NM_016830.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005840.2. On Jun 2, 2019 this sequence version replaced NM_016830.3. Transcript Variant: This variant (3), also known as VAMP-1B, contains an alternate 3' terminal exon, resulting in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 1. Isoforms 3 and 4 are the same length but differ in their C-termini. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.2897.1, SRR1163655.267688.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GGCGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058439.1
CDS	147..497
Translation	MSAPAQPPAEGTEGTAPGGGPPGPPPNMTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCKMMIMLGAICAIIVVVIVRRD

Target ORF information:

RefSeq Version	NM_016830.4
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016830.4

ORF Insert Sequence:

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GGCGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP1 ( NM_001297438.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284367.1 Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu28796
Clone ID Related Accession (Same CDS sequence)	NM_001297438.1 , NM_001297438.2
Accession Version	NM_001297438.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR749485.1, AC005840.2, CR989458.1 and BC023286.1. On Jul 3, 2014 this sequence version replaced XM_006719014.1. Transcript Variant: This variant (4) contains an alternate 3' terminal exon, resulting in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (4) has a distinct C-terminus and is shorter than isoform 1. Isoforms 3 and 4 are the same length but differ in their C-termini. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR989458.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GAAGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284367.1
CDS	369..719
Translation	MSAPAQPPAEGTEGTAPGGGPPGPPPNMTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCKMMIMLGAICAIIVVVIVRRG

Target ORF information:

RefSeq Version	NM_001297438.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297438.1

ORF Insert Sequence:

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GAAGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP1 ( NM_001297438.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284367.1 Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu28796
Clone ID Related Accession (Same CDS sequence)	NM_001297438.1 , NM_001297438.2
Accession Version	NM_001297438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005840.2. On Jun 2, 2019 this sequence version replaced NM_001297438.1. Transcript Variant: This variant (4) contains an alternate 3' terminal exon, resulting in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (4) has a distinct C-terminus and is shorter than isoform 1. Isoforms 3 and 4 are the same length but differ in their C-termini. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR989458.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GAAGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284367.1
CDS	147..497
Translation	MSAPAQPPAEGTEGTAPGGGPPGPPPNMTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCKMMIMLGAICAIIVVVIVRRG

Target ORF information:

RefSeq Version	NM_001297438.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 1 (VAMP1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297438.2

ORF Insert Sequence:

ATGTCTGCTC CAGCTCAGCC ACCTGCTGAA GGGACAGAAG GGACTGCCCC AGGTGGGGGT 
CCCCCTGGCC CTCCTCCTAA CATGACCAGT AACAGACGAC TACAGCAAAC CCAGGCACAA 
GTGGAGGAGG TGGTGGACAT CATACGTGTG AACGTGGACA AGGTCCTGGA GAGGGACCAG 
AAGCTGTCAG AGCTGGATGA CCGAGCTGAT GCCTTGCAGG CAGGAGCATC ACAATTTGAG 
AGCAGTGCTG CCAAGCTAAA GAGGAAGTAT TGGTGGAAAA ACTGCAAGAT GATGATCATG 
CTGGGAGCCA TCTGTGCCAT CATCGTGGTA GTTATTGTAA GAAGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Homozygous mutations in VAMP1 cause a presynaptic congenital myasthenic syndrome.
Annals of neurology81(4)597-603(2017 04)
Salpietro V,Lin W,Delle Vedove A,Storbeck M,Liu Y,Efthymiou S,Manole A,Wiethoff S,Ye Q,Saggar A,McElreavey K,Krishnakumar SS,,Pitt M,Bello OD,Rothman JE,Basel-Vanagaite L,Hubshman MW,Aharoni S,Manzur AY,Wirth B,Houlden H

Genetically-controlled Vesicle-Associated Membrane Protein 1 expression may contribute to Alzheimer's pathophysiology and susceptibility.
Molecular neurodegeneration1018(2015 Apr)
Sevlever D,Zou F,Ma L,Carrasquillo S,Crump MG,Culley OJ,Hunter TA,Bisceglio GD,Younkin L,Allen M,Carrasquillo MM,Sando SB,Aasly JO,Dickson DW,Graff-Radford NR,Petersen RC,De?k F,,Belbin O

Widespread sequence variations in VAMP1 across vertebrates suggest a potential selective pressure from botulinum neurotoxins.
PLoS pathogens10(7)e1004177(2014 Jul)
Peng L,Adler M,Demogines A,Borrell A,Liu H,Tao L,Tepp WH,Zhang SC,Johnson EA,Sawyer SL,Dong M

Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration.
Neuroscience266102-15(2014 Apr)
Manca P,Mameli O,Caria MA,Torrej?n-Escribano B,Blasi J

VAMP1 mutation causes dominant hereditary spastic ataxia in Newfoundland families.
American journal of human genetics91(3)548-52(2012 Sep)
Bourassa CV,Meijer IA,Merner ND,Grewal KK,Stefanelli MG,Hodgkinson K,Ives EJ,Pryse-Phillips W,Jog M,Boycott K,Grimes DA,Goobie S,Leckey R,Dion PA,Rouleau GA

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VAMP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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