SUPT4H1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SUPT4H1
Entrez Gene ID	6827
Full Name	SPT4 homolog, DSIF elongation factor subunit
Synonyms	SPT4,SPT4H,SUPT4H,Supt4a
General protein information	Preferred Names SPT4 homolog, DSIF elongation factor subunit Names transcription elongation factor SPT4 DRB sensitivity-inducing factor 14 kDa subunit DSIF p14 small hSpt4 subunit suppressor of Ty 4 homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q22
Summary	This gene encodes the small subunit of DRB (5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012].
Disorder MIM:	603555

mRNA and Protein(s)

mRNA	Protein	Name
NM_003168.3	NP_003159.1	transcription elongation factor SPT4
NM_003168.2	NP_003159.1	transcription elongation factor SPT4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SUPT4H1	XP_523811.2
	SUPT4H1	XP_001106749.1
Drosophila melanogaster (fruit fly)	spt4	NP_610802.1
Caenorhabditis elegans (roundworm)	spt-4	NP_497135.1
Mus musculus (house mouse)	Supt4a	NP_033322.1
Danio rerio (zebrafish)	supt4h1	NP_001002509.1
Bos taurus (cattle)	SUPT4H1	NP_001029964.1
Rattus norvegicus (Norway rat)	Supt4h1	NP_001099298.2
Neurospora crassa	NCU03838	XP_960597.1
Xenopus tropicalis (tropical clawed frog)	supt4h1	NP_001016679.1
Magnaporthe oryzae (rice blast fungus)	MGG_06943	XP_003709683.1
Homo sapiens (human)	SUPT4H1	NP_003159.1
Canis lupus familiaris (dog)	SUPT4H1	XP_853156.2
Gallus gallus (chicken)	SUPT4H1	NP_001244215.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005428	XP_315434.1

Gene Ontology
Bibliography

Related articles in PubMed

Spt4 selectively regulates the expression of C9orf72 sense and antisense mutant transcripts.
Kramer NJ, Carlomagno Y, Zhang YJ, Almeida S, Cook CN, Gendron TF, Prudencio M, Van Blitterswijk M, Belzil V, Couthouis J, Paul JW 3rd, Goodman LD, Daughrity L, Chew J, Garrett A, Pregent L, Jansen-West K, Tabassian LJ, Rademakers R, Boylan K, Graff-Radford NR, Josephs KA, Parisi JE, Knopman DS, Petersen RC, Boeve BF, Deng N, Feng Y, Cheng TH, Dickson DW, Cohen SN, Bonini NM, Link CD, Gao FB, Petrucelli L, Gitler AD
Science (New York, N.Y.)353(6300)708-12(2016 Aug)

Transcriptome analysis of paired primary colorectal carcinoma and liver metastases reveals fusion transcripts and similar gene expression profiles in primary carcinoma and liver metastases.
Lee JR, Kwon CH, Choi Y, Park HJ, Kim HS, Jo HJ, Oh N, Park do Y
BMC cancer16539(2016 07)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

Structure of transcribing mammalian RNA polymerase II.
Bernecky C, Herzog F, Baumeister W, Plitzko JM, Cramer P
Nature529(7587)551-4(2016 Jan)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of SUPT4H1, the human Spt4 ortholog, similarly decreased production of sense and antisense RNA foci, as well as dipeptide repeat proteins, in patient cells.
Title: Spt4 selectively regulates the expression of C9orf72 sense and antisense mutant transcripts.

Chimeric transcript resulting from the fusion of RNF43 and SUPT4H1 was found to occur frequently in primary colorectal carcinoma.
Title: Transcriptome analysis of paired primary colorectal carcinoma and liver metastases reveals fusion transcripts and similar gene expression profiles in primary carcinoma and liver metastases.

he Paf1 complex (Paf1C) and Tat-SF1, two factors implicated previously in elongation control, collaborate with DSIF to facilitate efficient elongation
Title: DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation.

crystal structure of hSpt4 in complex with the dimerization region of hSpt5
Title: Crystal structure of the human transcription elongation factor DSIF hSpt4 subunit in complex with the hSpt5 dimerization interface.

These results suggest that one of the functions of Spt5 is to suppress senescence and apoptosis, and that this function is exerted through its association with Spt4 and Pol II.
Title: Role of human transcription elongation factor DSIF in the suppression of senescence and apoptosis.

The following SUPT4H1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SUPT4H1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28469	NM_003168.3 Latest version!	Homo sapiens SPT4 homolog, DSIF elongation factor subunit (SUPT4H1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu28469	NM_003168.2	Homo sapiens SPT4 homolog, DSIF elongation factor subunit (SUPT4H1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SUPT4H1 ( NM_003168.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003159.1 Homo sapiens SPT4 homolog, DSIF elongation factor subunit (SUPT4H1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28469
Clone ID Related Accession (Same CDS sequence)	NM_003168.3 , NM_003168.2
Accession Version	NM_003168.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	transcription elongation factor SPT4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U43923.1 and BG026473.1. On Nov 22, 2018 this sequence version replaced NM_003168.2. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43923.1, SRR5189652.103830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000225504.8/ ENSP00000225504.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCTGG AGACGGTGCC GAAGGACCTG CGGCATCTGC GGGCCTGTTT GCTGTGTTCG 
CTGGTCAAGA CTATAGACCA GTTTGAATAT GATGGTTGTG ACAATTGTGA TGCATATCTA 
CAAATGAAGG GTAACCGAGA GATGGTATAT GACTGCACTA GCTCTTCCTT TGATGGAATC 
ATTGCGATGA TGAGTCCAGA GGACAGCTGG GTCTCCAAGT GGCAGCGAGT CAGTAACTTT 
AAGCCAGGTG TATATGCGGT GTCAGTCACT GGTCGCCTGC CCCAAGGAAT CGTGCGGGAG 
CTGAAAAGTC GAGGAGTGGC CTACAAATCC AGAGACACAG CTATAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003159.1
CDS	67..420
Translation	MALETVPKDLRHLRACLLCSLVKTIDQFEYDGCDNCDAYLQMKGNREMVYDCTSSSFDGIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT

Target ORF information:

RefSeq Version	NM_003168.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SPT4 homolog, DSIF elongation factor subunit (SUPT4H1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003168.3

ORF Insert Sequence:

ATGGCCCTGG AGACGGTGCC GAAGGACCTG CGGCATCTGC GGGCCTGTTT GCTGTGTTCG 
CTGGTCAAGA CTATAGACCA GTTTGAATAT GATGGTTGTG ACAATTGTGA TGCATATCTA 
CAAATGAAGG GTAACCGAGA GATGGTATAT GACTGCACTA GCTCTTCCTT TGATGGAATC 
ATTGCGATGA TGAGTCCAGA GGACAGCTGG GTCTCCAAGT GGCAGCGAGT CAGTAACTTT 
AAGCCAGGTG TATATGCGGT GTCAGTCACT GGTCGCCTGC CCCAAGGAAT CGTGCGGGAG 
CTGAAAAGTC GAGGAGTGGC CTACAAATCC AGAGACACAG CTATAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SUPT4H1 ( NM_003168.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003159.1 Homo sapiens SPT4 homolog, DSIF elongation factor subunit (SUPT4H1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28469
Clone ID Related Accession (Same CDS sequence)	NM_003168.3 , NM_003168.2
Accession Version	NM_003168.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-08
Organism	Homo sapiens(human)
Product	transcription elongation factor SPT4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA311965.1, U43923.1, BG026473.1 and AI342302.1. On Nov 29, 2012 this sequence version replaced NM_003168.1. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP196741.1, U43923.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGG AGACGGTGCC GAAGGACCTG CGGCATCTGC GGGCCTGTTT GCTGTGTTCG 
CTGGTCAAGA CTATAGACCA GTTTGAATAT GATGGTTGTG ACAATTGTGA TGCATATCTA 
CAAATGAAGG GTAACCGAGA GATGGTATAT GACTGCACTA GCTCTTCCTT TGATGGAATC 
ATTGCGATGA TGAGTCCAGA GGACAGCTGG GTCTCCAAGT GGCAGCGAGT CAGTAACTTT 
AAGCCAGGTG TATATGCGGT GTCAGTCACT GGTCGCCTGC CCCAAGGAAT CGTGCGGGAG 
CTGAAAAGTC GAGGAGTGGC CTACAAATCC AGAGACACAG CTATAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003159.1
CDS	104..457
Translation	MALETVPKDLRHLRACLLCSLVKTIDQFEYDGCDNCDAYLQMKGNREMVYDCTSSSFDGIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT

Target ORF information:

RefSeq Version	NM_003168.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SPT4 homolog, DSIF elongation factor subunit (SUPT4H1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003168.2

ORF Insert Sequence:

ATGGCCCTGG AGACGGTGCC GAAGGACCTG CGGCATCTGC GGGCCTGTTT GCTGTGTTCG 
CTGGTCAAGA CTATAGACCA GTTTGAATAT GATGGTTGTG ACAATTGTGA TGCATATCTA 
CAAATGAAGG GTAACCGAGA GATGGTATAT GACTGCACTA GCTCTTCCTT TGATGGAATC 
ATTGCGATGA TGAGTCCAGA GGACAGCTGG GTCTCCAAGT GGCAGCGAGT CAGTAACTTT 
AAGCCAGGTG TATATGCGGT GTCAGTCACT GGTCGCCTGC CCCAAGGAAT CGTGCGGGAG 
CTGAAAAGTC GAGGAGTGGC CTACAAATCC AGAGACACAG CTATAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spt4 selectively regulates the expression of C9orf72 sense and antisense mutant transcripts.
Science (New York, N.Y.)353(6300)708-12(2016 Aug)
Kramer NJ,Carlomagno Y,Zhang YJ,Almeida S,Cook CN,Gendron TF,Prudencio M,Van Blitterswijk M,Belzil V,Couthouis J,Paul JW 3rd,Goodman LD,Daughrity L,Chew J,Garrett A,Pregent L,Jansen-West K,Tabassian LJ,Rademakers R,Boylan K,Graff-Radford NR,Josephs KA,Parisi JE,Knopman DS,Petersen RC,Boeve BF,Deng N,Feng Y,Cheng TH,Dickson DW,Cohen SN,Bonini NM,Link CD,Gao FB,Petrucelli L,Gitler AD

Transcriptome analysis of paired primary colorectal carcinoma and liver metastases reveals fusion transcripts and similar gene expression profiles in primary carcinoma and liver metastases.
BMC cancer16539(2016 07)
Lee JR,Kwon CH,Choi Y,Park HJ,Kim HS,Jo HJ,Oh N,Park do Y

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

Structure of transcribing mammalian RNA polymerase II.
Nature529(7587)551-4(2016 Jan)
Bernecky C,Herzog F,Baumeister W,Plitzko JM,Cramer P

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

SUPT4H1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報