NEK4 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011534039.3	XP_011532341.1	serine/threonine-protein kinase Nek4 isoform X2
NM_001348413.2	NP_001335342.1	serine/threonine-protein kinase Nek4 isoform 4
NM_001348414.2	NP_001335343.1	serine/threonine-protein kinase Nek4 isoform 5
NM_001348412.2	NP_001335341.1	serine/threonine-protein kinase Nek4 isoform 3
NM_001193533.2	NP_001180462.1	serine/threonine-protein kinase Nek4 isoform 2
XM_011534039.2	XP_011532341.1	serine/threonine-protein kinase Nek4 isoform X2
NM_001348412.1	NP_001335341.1	serine/threonine-protein kinase Nek4 isoform 3
NM_001348414.1	NP_001335343.1	serine/threonine-protein kinase Nek4 isoform 5
XM_017007086.1	XP_016862575.1	serine/threonine-protein kinase Nek4 isoform X3
NM_003157.6	NP_003148.2	serine/threonine-protein kinase Nek4 isoform 1
NM_001348413.1	NP_001335342.1	serine/threonine-protein kinase Nek4 isoform 4
NM_003157.5	NP_003148.2	serine/threonine-protein kinase Nek4 isoform 1
XM_011534040.2	XP_011532342.1	serine/threonine-protein kinase Nek4 isoform X5
XM_017007085.1	XP_016862574.1	serine/threonine-protein kinase Nek4 isoform X1
XM_011534040.3	XP_011532342.1	serine/threonine-protein kinase Nek4 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28130	NM_001193533.2 Latest version!	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$379.50-$531.30 $759.00
OHu28201	NM_003157.6 Latest version!	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$594.30 $849.00
OHu37441	NM_001348412.2 Latest version!	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu60776	XM_011534039.3 Latest version!	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu60777	XM_011534040.3 Latest version!	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu102245	NM_001348414.2 Latest version!	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu102374	NM_001348413.2 Latest version!	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu94523	XM_017007085.1 Latest version!	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu94524	XM_017007086.1 Latest version!	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu60776	XM_011534039.2	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu37441	NM_001348412.1	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu102245	NM_001348414.1	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu102374	NM_001348413.1	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu28201	NM_003157.5	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$594.30 $849.00
OHu60777	XM_011534040.2	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

NEK4 ( NM_001193533.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180462.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28130
Clone ID Related Accession (Same CDS sequence)	NM_001193533.2
Accession Version	NM_001193533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB073127.1, AK294165.1, L20321.1 and AC104446.2. On Feb 1, 2017 this sequence version replaced NM_001193533.1. Transcript Variant: This variant (2) lacks an alternate, in-frame, 5' coding region segment, compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294165.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATTTAC ATGAAAAACA CATCCTTCAT 
CGAGATCTGA AAACTCAAAA TGTCTTCCTA ACAAGAACAA ACATCATCAA AGTAGGGGAC 
CTAGGAATTG CCCGAGTGTT AGAGAACCAC TGTGACATGG CTAGCACCCT CATTGGCACA 
CCCTACTACA TGAGCCCTGA ATTGTTCTCA AACAAACCCT ACAACTATAA GTCTGATGTT 
TGGGCTCTAG GATGCTGTGT CTATGAAATG GCCACCTTGA AGCATGCTTT CAATGCAAAA 
GATATGAATT CTTTAGTTTA TCGGATTATT GAAGGAAAGC TGCCACCAAT GCCAAGAGAT 
TACAGCCCAG AGCTGGCAGA ACTGATAAGA ACAATGCTGA GCAAAAGGCC TGAAGAAAGG 
CCGTCTGTGA GGAGCATCCT GAGGCAGCCT TATATAAAGC GGCAAATCTC CTTCTTTTTG 
GAGGCCACAA AGATAAAAAC CTCCAAAAAT AACATTAAAA ATGGTGACTC TCAATCCAAG 
CCTTTTGCTA CAGTGGTTTC TGGAGAGGCA GAATCAAATC ATGAAGTAAT CCACCCCCAA 
CCACTCTCTT CTGAGGGCTC CCAGACATAT ATAATGGGTG AAGGCAAATG TTTGTCCCAG 
GAGAAACCCA GGGCCTCTGG TCTCTTGAAG TCACCTGCCA GTCTGAAAGC CCATACCTGC 
AAACAGGACT TGAGCAATAC CACAGAACTA GCCACAATCA GTAGCGTAAA TATTGACATC 
TTACCTGCAA AAGGGAGGGA TTCAGTGAGT GATGGCTTTG TTCAGGAGAA TCAGCCAAGA 
TATTTGGATG CCTCTAATGA GTTAGGAGGT ATATGCAGTA TTTCTCAAGT GGAAGAGGAG 
ATGCTGCAGG ACAACACTAA ATCCAGTGCC CAGCCTGAAA ACCTGATTCC CATGTGGTCC 
TCTGACATTG TCACTGGGGA AAAGAATGAA CCAGTGAAGC CTCTGCAGCC CCTAATCAAA 
GAACAAAAGC CAAAGGACCA GAGTCTTGCC CTGTCGCCCA AGCTGGAGTG CAGTGGCACA 
ATCTTGGCTC ACAGCAACCT CCGCCTCCTG GGTTCAAGTG ATTCTCCAGC CTCAGCCTCC 
CGAGTAGCTG GGATTACAGG CGTGTGCCAC CACGCCCAGG ATCAAGTTGC TGGTGAATGT 
ATTATAGAAA AACAGGGCAG AATCCACCCA GATTTACAGC CACACAACTC TGGGTCTGAA 
CCTTCCCTGT CTCGACAGCG ACGGCAAAAG AGGAGAGAAC AGACTGAGCA CAGAGGGGAA 
AAGAGACAGG TCCGCAGAGA TCTCTTTGCT TTCCAAGAGT CGCCTCCTCG ATTTTTGCCT 
TCTCATCCCA TTGTTGGGAA AGTGGATGTC ACATCAACAC AAAAAGAGGC TGAAAACCAA 
CGTAGAGTGG TCACTGGGTC TGTGAGCAGT TCAAGGAGCA GTGAGATGTC ATCATCAAAG 
GATCGACCAT TATCAGCCAG AGAGAGGAGG CGACTAAAGC AGTCACAGGA AGAAATGTCC 
TCTTCAGGCC CTTCAGTGAG GAAAGCGTCT CTGAGTGTAG CAGGGCCAGG AAAACCCCAG 
GAAGAAGACC AGCCCTTGCC TGCCCGACGG CTCTCCTCTG ACTGCAGCGT CACTCAGGAA 
AGGAAACAGA TTCATTGTCT GTCTGAGGAT GAGTTAAGTT CTTCTACAAG TTCAACTGAT 
AAGTCAGATG GGGATTACGG GGAAGGGAAA GGTCAGACAA ATGAAATTAA TGCCTTGGTA 
CAATTGATGA CTCAGACCCT GAAACTGGAT TCTAAAGAGA GCTGTGAAGA TGTCCCGGTA 
GCAAACCCAG TGTCAGAATT CAAACTTCAT CGGAAATATC GGGACACACT GATACTTCAT 
GGGAAGGTTG CAGAAGAGGC AGAGGAAATC CATTTTAAAG AGCTACCTTC AGCTATTATG 
CCAGGTTCTG AAAAGATCAG GAGACTAGTT GAAGTCTTGA GAACTGATGT AATTCGTGGC 
CTGGGAGTTC AGCTTTTAGA GCAGGTGTAT GATCTTTTGG AGGAGGAGGA TGAATTTGAT 
AGAGAGGTAC GTTTGCGGGA GCACATGGGT GAAAAGTATA CAACTTACAG TGTGAAAGCT 
CGCCAGTTGA AATTTTTTGA AGAAAACATG AATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180462.1
CDS	204..2462
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMNF

Target ORF information:

RefSeq Version	NM_001193533.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193533.2

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATTTAC ATGAAAAACA CATCCTTCAT 
CGAGATCTGA AAACTCAAAA TGTCTTCCTA ACAAGAACAA ACATCATCAA AGTAGGGGAC 
CTAGGAATTG CCCGAGTGTT AGAGAACCAC TGTGACATGG CTAGCACCCT CATTGGCACA 
CCCTACTACA TGAGCCCTGA ATTGTTCTCA AACAAACCCT ACAACTATAA GTCTGATGTT 
TGGGCTCTAG GATGCTGTGT CTATGAAATG GCCACCTTGA AGCATGCTTT CAATGCAAAA 
GATATGAATT CTTTAGTTTA TCGGATTATT GAAGGAAAGC TGCCACCAAT GCCAAGAGAT 
TACAGCCCAG AGCTGGCAGA ACTGATAAGA ACAATGCTGA GCAAAAGGCC TGAAGAAAGG 
CCGTCTGTGA GGAGCATCCT GAGGCAGCCT TATATAAAGC GGCAAATCTC CTTCTTTTTG 
GAGGCCACAA AGATAAAAAC CTCCAAAAAT AACATTAAAA ATGGTGACTC TCAATCCAAG 
CCTTTTGCTA CAGTGGTTTC TGGAGAGGCA GAATCAAATC ATGAAGTAAT CCACCCCCAA 
CCACTCTCTT CTGAGGGCTC CCAGACATAT ATAATGGGTG AAGGCAAATG TTTGTCCCAG 
GAGAAACCCA GGGCCTCTGG TCTCTTGAAG TCACCTGCCA GTCTGAAAGC CCATACCTGC 
AAACAGGACT TGAGCAATAC CACAGAACTA GCCACAATCA GTAGCGTAAA TATTGACATC 
TTACCTGCAA AAGGGAGGGA TTCAGTGAGT GATGGCTTTG TTCAGGAGAA TCAGCCAAGA 
TATTTGGATG CCTCTAATGA GTTAGGAGGT ATATGCAGTA TTTCTCAAGT GGAAGAGGAG 
ATGCTGCAGG ACAACACTAA ATCCAGTGCC CAGCCTGAAA ACCTGATTCC CATGTGGTCC 
TCTGACATTG TCACTGGGGA AAAGAATGAA CCAGTGAAGC CTCTGCAGCC CCTAATCAAA 
GAACAAAAGC CAAAGGACCA GAGTCTTGCC CTGTCGCCCA AGCTGGAGTG CAGTGGCACA 
ATCTTGGCTC ACAGCAACCT CCGCCTCCTG GGTTCAAGTG ATTCTCCAGC CTCAGCCTCC 
CGAGTAGCTG GGATTACAGG CGTGTGCCAC CACGCCCAGG ATCAAGTTGC TGGTGAATGT 
ATTATAGAAA AACAGGGCAG AATCCACCCA GATTTACAGC CACACAACTC TGGGTCTGAA 
CCTTCCCTGT CTCGACAGCG ACGGCAAAAG AGGAGAGAAC AGACTGAGCA CAGAGGGGAA 
AAGAGACAGG TCCGCAGAGA TCTCTTTGCT TTCCAAGAGT CGCCTCCTCG ATTTTTGCCT 
TCTCATCCCA TTGTTGGGAA AGTGGATGTC ACATCAACAC AAAAAGAGGC TGAAAACCAA 
CGTAGAGTGG TCACTGGGTC TGTGAGCAGT TCAAGGAGCA GTGAGATGTC ATCATCAAAG 
GATCGACCAT TATCAGCCAG AGAGAGGAGG CGACTAAAGC AGTCACAGGA AGAAATGTCC 
TCTTCAGGCC CTTCAGTGAG GAAAGCGTCT CTGAGTGTAG CAGGGCCAGG AAAACCCCAG 
GAAGAAGACC AGCCCTTGCC TGCCCGACGG CTCTCCTCTG ACTGCAGCGT CACTCAGGAA 
AGGAAACAGA TTCATTGTCT GTCTGAGGAT GAGTTAAGTT CTTCTACAAG TTCAACTGAT 
AAGTCAGATG GGGATTACGG GGAAGGGAAA GGTCAGACAA ATGAAATTAA TGCCTTGGTA 
CAATTGATGA CTCAGACCCT GAAACTGGAT TCTAAAGAGA GCTGTGAAGA TGTCCCGGTA 
GCAAACCCAG TGTCAGAATT CAAACTTCAT CGGAAATATC GGGACACACT GATACTTCAT 
GGGAAGGTTG CAGAAGAGGC AGAGGAAATC CATTTTAAAG AGCTACCTTC AGCTATTATG 
CCAGGTTCTG AAAAGATCAG GAGACTAGTT GAAGTCTTGA GAACTGATGT AATTCGTGGC 
CTGGGAGTTC AGCTTTTAGA GCAGGTGTAT GATCTTTTGG AGGAGGAGGA TGAATTTGAT 
AGAGAGGTAC GTTTGCGGGA GCACATGGGT GAAAAGTATA CAACTTACAG TGTGAAAGCT 
CGCCAGTTGA AATTTTTTGA AGAAAACATG AATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( NM_003157.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_003148.2 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28201
Clone ID Related Accession (Same CDS sequence)	NM_003157.6 , NM_003157.5
Accession Version	NM_003157.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB073127.1, BC015515.1, L20321.1 and AC104446.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_003157.5. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L20321.1, SRR1660805.187757.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000233027.10/ ENSP00000233027.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGAAAGG AAACAGATTC ATTGTCTGTC TGAGGATGAG 
TTAAGTTCTT CTACAAGTTC AACTGATAAG TCAGATGGGG ATTACGGGGA AGGGAAAGGT 
CAGACAAATG AAATTAATGC CTTGGTACAA TTGATGACTC AGACCCTGAA ACTGGATTCT 
AAAGAGAGCT GTGAAGATGT CCCGGTAGCA AACCCAGTGT CAGAATTCAA ACTTCATCGG 
AAATATCGGG ACACACTGAT ACTTCATGGG AAGGTTGCAG AAGAGGCAGA GGAAATCCAT 
TTTAAAGAGC TACCTTCAGC TATTATGCCA GGTTCTGAAA AGATCAGGAG ACTAGTTGAA 
GTCTTGAGAA CTGATGTAAT TCGTGGCCTG GGAGTTCAGC TTTTAGAGCA GGTGTATGAT 
CTTTTGGAGG AGGAGGATGA ATTTGATAGA GAGGTACGTT TGCGGGAGCA CATGGGTGAA 
AAGTATACAA CTTACAGTGT GAAAGCTCGC CAGTTGAAAT TTTTTGAAGA AAACATGAAT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003148.2
CDS	195..2720
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMNF

Target ORF information:

RefSeq Version	NM_003157.6
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003157.6

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGAAAGG AAACAGATTC ATTGTCTGTC TGAGGATGAG 
TTAAGTTCTT CTACAAGTTC AACTGATAAG TCAGATGGGG ATTACGGGGA AGGGAAAGGT 
CAGACAAATG AAATTAATGC CTTGGTACAA TTGATGACTC AGACCCTGAA ACTGGATTCT 
AAAGAGAGCT GTGAAGATGT CCCGGTAGCA AACCCAGTGT CAGAATTCAA ACTTCATCGG 
AAATATCGGG ACACACTGAT ACTTCATGGG AAGGTTGCAG AAGAGGCAGA GGAAATCCAT 
TTTAAAGAGC TACCTTCAGC TATTATGCCA GGTTCTGAAA AGATCAGGAG ACTAGTTGAA 
GTCTTGAGAA CTGATGTAAT TCGTGGCCTG GGAGTTCAGC TTTTAGAGCA GGTGTATGAT 
CTTTTGGAGG AGGAGGATGA ATTTGATAGA GAGGTACGTT TGCGGGAGCA CATGGGTGAA 
AAGTATACAA CTTACAGTGT GAAAGCTCGC CAGTTGAAAT TTTTTGAAGA AAACATGAAT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( NM_003157.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003148.2 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 1, mRNA.

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CloneID	OHu28201
Clone ID Related Accession (Same CDS sequence)	NM_003157.6 , NM_003157.5
Accession Version	NM_003157.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB073127.1, BC015515.1, L20321.1 and AC104446.2. This sequence is a reference standard in the RefSeqGene project. On Feb 8, 2017 this sequence version replaced NM_003157.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: L20321.1, SRR1660805.187757.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGAAAGG AAACAGATTC ATTGTCTGTC TGAGGATGAG 
TTAAGTTCTT CTACAAGTTC AACTGATAAG TCAGATGGGG ATTACGGGGA AGGGAAAGGT 
CAGACAAATG AAATTAATGC CTTGGTACAA TTGATGACTC AGACCCTGAA ACTGGATTCT 
AAAGAGAGCT GTGAAGATGT CCCGGTAGCA AACCCAGTGT CAGAATTCAA ACTTCATCGG 
AAATATCGGG ACACACTGAT ACTTCATGGG AAGGTTGCAG AAGAGGCAGA GGAAATCCAT 
TTTAAAGAGC TACCTTCAGC TATTATGCCA GGTTCTGAAA AGATCAGGAG ACTAGTTGAA 
GTCTTGAGAA CTGATGTAAT TCGTGGCCTG GGAGTTCAGC TTTTAGAGCA GGTGTATGAT 
CTTTTGGAGG AGGAGGATGA ATTTGATAGA GAGGTACGTT TGCGGGAGCA CATGGGTGAA 
AAGTATACAA CTTACAGTGT GAAAGCTCGC CAGTTGAAAT TTTTTGAAGA AAACATGAAT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003148.2
CDS	204..2729
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMNF

Target ORF information:

RefSeq Version	NM_003157.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003157.5

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGAAAGG AAACAGATTC ATTGTCTGTC TGAGGATGAG 
TTAAGTTCTT CTACAAGTTC AACTGATAAG TCAGATGGGG ATTACGGGGA AGGGAAAGGT 
CAGACAAATG AAATTAATGC CTTGGTACAA TTGATGACTC AGACCCTGAA ACTGGATTCT 
AAAGAGAGCT GTGAAGATGT CCCGGTAGCA AACCCAGTGT CAGAATTCAA ACTTCATCGG 
AAATATCGGG ACACACTGAT ACTTCATGGG AAGGTTGCAG AAGAGGCAGA GGAAATCCAT 
TTTAAAGAGC TACCTTCAGC TATTATGCCA GGTTCTGAAA AGATCAGGAG ACTAGTTGAA 
GTCTTGAGAA CTGATGTAAT TCGTGGCCTG GGAGTTCAGC TTTTAGAGCA GGTGTATGAT 
CTTTTGGAGG AGGAGGATGA ATTTGATAGA GAGGTACGTT TGCGGGAGCA CATGGGTGAA 
AAGTATACAA CTTACAGTGT GAAAGCTCGC CAGTTGAAAT TTTTTGAAGA AAACATGAAT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( NM_001348412.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335341.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu37441
Clone ID Related Accession (Same CDS sequence)	NM_001348412.2 , NM_001348412.1
Accession Version	NM_001348412.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006254.10 and AC104446.2. On Jun 1, 2019 this sequence version replaced NM_001348412.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.249465.1, SRR1660805.154143.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGGA TCAAGTTGCT 
GGTGAATGTA TTATAGAAAA ACAGGGCAGA ATCCACCCAG ATTTACAGCC ACACAACTCT 
GGGTCTGAAC CTTCCCTGTC TCGACAGCGA CGGCAAAAGA GGAGAGAACA GACTGAGCAC 
AGAGGGGAAA AGAGACAGGT CCGCAGAGAT CTCTTTGCTT TCCAAGAGTC GCCTCCTCGA 
TTTTTGCCTT CTCATCCCAT TGTTGGGAAA GTGGATGTCA CATCAACACA AAAAGAGGCT 
GAAAACCAAC GTAGAGTGGT CACTGGGTCT GTGAGCAGTT CAAGGAGCAG TGAGATGTCA 
TCATCAAAGG ATCGACCATT ATCAGCCAGA GAGAGGAGGC GACTAAAGCA GTCACAGGAA 
GAAATGTCCT CTTCAGGCCC TTCAGTGAGG AAAGCGTCTC TGAGTGTAGC AGGGCCAGGA 
AAACCCCAGG AAGAAGACCA GCCCTTGCCT GCCCGACGGC TCTCCTCTGA CTGCAGCGTC 
ACTCAGGAAA GGAAACAGAT TCATTGTCTG TCTGAGGATG AGTTAAGTTC TTCTACAAGT 
TCAACTGATA AGTCAGATGG GGATTACGGG GAAGGGAAAG GTCAGACAAA TGAAATTAAT 
GCCTTGGTAC AATTGATGAC TCAGACCCTG AAACTGGATT CTAAAGAGAG CTGTGAAGAT 
GTCCCGGTAG CAAACCCAGT GTCAGAATTC AAACTTCATC GGAAATATCG GGACACACTG 
ATACTTCATG GGAAGGTTGC AGAAGAGGCA GAGGAAATCC ATTTTAAAGA GCTACCTTCA 
GCTATTATGC CAGGTTCTGA AAAGATCAGG AGACTAGTTG AAGTCTTGAG AACTGATGTA 
ATTCGTGGCC TGGGAGTTCA GCTTTTAGAG CAGGTGTATG ATCTTTTGGA GGAGGAGGAT 
GAATTTGATA GAGAGGTACG TTTGCGGGAG CACATGGGTG AAAAGTATAC AACTTACAGT 
GTGAAAGCTC GCCAGTTGAA ATTTTTTGAA GAAAACATGA ATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335341.1
CDS	195..2582
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMNF

Target ORF information:

RefSeq Version	NM_001348412.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348412.2

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGGA TCAAGTTGCT 
GGTGAATGTA TTATAGAAAA ACAGGGCAGA ATCCACCCAG ATTTACAGCC ACACAACTCT 
GGGTCTGAAC CTTCCCTGTC TCGACAGCGA CGGCAAAAGA GGAGAGAACA GACTGAGCAC 
AGAGGGGAAA AGAGACAGGT CCGCAGAGAT CTCTTTGCTT TCCAAGAGTC GCCTCCTCGA 
TTTTTGCCTT CTCATCCCAT TGTTGGGAAA GTGGATGTCA CATCAACACA AAAAGAGGCT 
GAAAACCAAC GTAGAGTGGT CACTGGGTCT GTGAGCAGTT CAAGGAGCAG TGAGATGTCA 
TCATCAAAGG ATCGACCATT ATCAGCCAGA GAGAGGAGGC GACTAAAGCA GTCACAGGAA 
GAAATGTCCT CTTCAGGCCC TTCAGTGAGG AAAGCGTCTC TGAGTGTAGC AGGGCCAGGA 
AAACCCCAGG AAGAAGACCA GCCCTTGCCT GCCCGACGGC TCTCCTCTGA CTGCAGCGTC 
ACTCAGGAAA GGAAACAGAT TCATTGTCTG TCTGAGGATG AGTTAAGTTC TTCTACAAGT 
TCAACTGATA AGTCAGATGG GGATTACGGG GAAGGGAAAG GTCAGACAAA TGAAATTAAT 
GCCTTGGTAC AATTGATGAC TCAGACCCTG AAACTGGATT CTAAAGAGAG CTGTGAAGAT 
GTCCCGGTAG CAAACCCAGT GTCAGAATTC AAACTTCATC GGAAATATCG GGACACACTG 
ATACTTCATG GGAAGGTTGC AGAAGAGGCA GAGGAAATCC ATTTTAAAGA GCTACCTTCA 
GCTATTATGC CAGGTTCTGA AAAGATCAGG AGACTAGTTG AAGTCTTGAG AACTGATGTA 
ATTCGTGGCC TGGGAGTTCA GCTTTTAGAG CAGGTGTATG ATCTTTTGGA GGAGGAGGAT 
GAATTTGATA GAGAGGTACG TTTGCGGGAG CACATGGGTG AAAAGTATAC AACTTACAGT 
GTGAAAGCTC GCCAGTTGAA ATTTTTTGAA GAAAACATGA ATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( NM_001348412.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335341.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 3, mRNA.

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CloneID	OHu37441
Clone ID Related Accession (Same CDS sequence)	NM_001348412.2 , NM_001348412.1
Accession Version	NM_001348412.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-28
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006254.10 and AC104446.2. On Feb 1, 2017 this sequence version replaced XM_006713310.2. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.249465.1, SRR1660803.215026.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGGA TCAAGTTGCT 
GGTGAATGTA TTATAGAAAA ACAGGGCAGA ATCCACCCAG ATTTACAGCC ACACAACTCT 
GGGTCTGAAC CTTCCCTGTC TCGACAGCGA CGGCAAAAGA GGAGAGAACA GACTGAGCAC 
AGAGGGGAAA AGAGACAGGT CCGCAGAGAT CTCTTTGCTT TCCAAGAGTC GCCTCCTCGA 
TTTTTGCCTT CTCATCCCAT TGTTGGGAAA GTGGATGTCA CATCAACACA AAAAGAGGCT 
GAAAACCAAC GTAGAGTGGT CACTGGGTCT GTGAGCAGTT CAAGGAGCAG TGAGATGTCA 
TCATCAAAGG ATCGACCATT ATCAGCCAGA GAGAGGAGGC GACTAAAGCA GTCACAGGAA 
GAAATGTCCT CTTCAGGCCC TTCAGTGAGG AAAGCGTCTC TGAGTGTAGC AGGGCCAGGA 
AAACCCCAGG AAGAAGACCA GCCCTTGCCT GCCCGACGGC TCTCCTCTGA CTGCAGCGTC 
ACTCAGGAAA GGAAACAGAT TCATTGTCTG TCTGAGGATG AGTTAAGTTC TTCTACAAGT 
TCAACTGATA AGTCAGATGG GGATTACGGG GAAGGGAAAG GTCAGACAAA TGAAATTAAT 
GCCTTGGTAC AATTGATGAC TCAGACCCTG AAACTGGATT CTAAAGAGAG CTGTGAAGAT 
GTCCCGGTAG CAAACCCAGT GTCAGAATTC AAACTTCATC GGAAATATCG GGACACACTG 
ATACTTCATG GGAAGGTTGC AGAAGAGGCA GAGGAAATCC ATTTTAAAGA GCTACCTTCA 
GCTATTATGC CAGGTTCTGA AAAGATCAGG AGACTAGTTG AAGTCTTGAG AACTGATGTA 
ATTCGTGGCC TGGGAGTTCA GCTTTTAGAG CAGGTGTATG ATCTTTTGGA GGAGGAGGAT 
GAATTTGATA GAGAGGTACG TTTGCGGGAG CACATGGGTG AAAAGTATAC AACTTACAGT 
GTGAAAGCTC GCCAGTTGAA ATTTTTTGAA GAAAACATGA ATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335341.1
CDS	204..2591
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMNF

Target ORF information:

RefSeq Version	NM_001348412.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348412.1

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGGA TCAAGTTGCT 
GGTGAATGTA TTATAGAAAA ACAGGGCAGA ATCCACCCAG ATTTACAGCC ACACAACTCT 
GGGTCTGAAC CTTCCCTGTC TCGACAGCGA CGGCAAAAGA GGAGAGAACA GACTGAGCAC 
AGAGGGGAAA AGAGACAGGT CCGCAGAGAT CTCTTTGCTT TCCAAGAGTC GCCTCCTCGA 
TTTTTGCCTT CTCATCCCAT TGTTGGGAAA GTGGATGTCA CATCAACACA AAAAGAGGCT 
GAAAACCAAC GTAGAGTGGT CACTGGGTCT GTGAGCAGTT CAAGGAGCAG TGAGATGTCA 
TCATCAAAGG ATCGACCATT ATCAGCCAGA GAGAGGAGGC GACTAAAGCA GTCACAGGAA 
GAAATGTCCT CTTCAGGCCC TTCAGTGAGG AAAGCGTCTC TGAGTGTAGC AGGGCCAGGA 
AAACCCCAGG AAGAAGACCA GCCCTTGCCT GCCCGACGGC TCTCCTCTGA CTGCAGCGTC 
ACTCAGGAAA GGAAACAGAT TCATTGTCTG TCTGAGGATG AGTTAAGTTC TTCTACAAGT 
TCAACTGATA AGTCAGATGG GGATTACGGG GAAGGGAAAG GTCAGACAAA TGAAATTAAT 
GCCTTGGTAC AATTGATGAC TCAGACCCTG AAACTGGATT CTAAAGAGAG CTGTGAAGAT 
GTCCCGGTAG CAAACCCAGT GTCAGAATTC AAACTTCATC GGAAATATCG GGACACACTG 
ATACTTCATG GGAAGGTTGC AGAAGAGGCA GAGGAAATCC ATTTTAAAGA GCTACCTTCA 
GCTATTATGC CAGGTTCTGA AAAGATCAGG AGACTAGTTG AAGTCTTGAG AACTGATGTA 
ATTCGTGGCC TGGGAGTTCA GCTTTTAGAG CAGGTGTATG ATCTTTTGGA GGAGGAGGAT 
GAATTTGATA GAGAGGTACG TTTGCGGGAG CACATGGGTG AAAAGTATAC AACTTACAGT 
GTGAAAGCTC GCCAGTTGAA ATTTTTTGAA GAAAACATGA ATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_011534039.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532341.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu60776
Clone ID Related Accession (Same CDS sequence)	XM_011534039.3 , XM_011534039.2
Accession Version	XM_011534039.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011534039.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGAAAGG AAACAGATTC ATTGTCTGTC TGAGGATGAG 
TTAAGTTCTT CTACAAGTTC AACTGATAAG TCAGATGGGG ATTACGGGGA AGGGAAAGGT 
CAGACAAATG AAATTAATGC CTTGGTACAA TTGATGACTC AGACCCTGAA ACTGGATTCT 
AAAGAGAGCT GTGAAGATGT CCCGGTAGCA AACCCAGTGT CAGAATTCAA ACTTCATCGG 
AAATATCGGG ACACACTGAT ACTTCATGGG AAGGTTGCAG AAGAGGCAGA GGAAATCCAT 
TTTAAAGAGC TACCTTCAGC TATTATGCCA GGTTCTGAAA AGATCAGGAG ACTAGTTGAA 
GTCTTGAGAA CTGATGTAAT TCGTGGCCTG GGAGTTCAGC TTTTAGAGCA GGTGTATGAT 
CTTTTGGAGG AGGAGGATGA ATTTGATAGA GAGTGTCCCT GTCTGGCCCC AGCACCAGGG 
CATTGTCGTC AGCCTCATAA AATGAACCAG GAATTACCCC TTCCTCCTCA ACTCTCTGGA 
AAAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532341.1
CDS	201..2729
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDRECPCLAPAPGHCRQPHKMNQELPLPPQLSGKV

Target ORF information:

RefSeq Version	XM_011534039.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534039.3

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGAAAGG AAACAGATTC ATTGTCTGTC TGAGGATGAG 
TTAAGTTCTT CTACAAGTTC AACTGATAAG TCAGATGGGG ATTACGGGGA AGGGAAAGGT 
CAGACAAATG AAATTAATGC CTTGGTACAA TTGATGACTC AGACCCTGAA ACTGGATTCT 
AAAGAGAGCT GTGAAGATGT CCCGGTAGCA AACCCAGTGT CAGAATTCAA ACTTCATCGG 
AAATATCGGG ACACACTGAT ACTTCATGGG AAGGTTGCAG AAGAGGCAGA GGAAATCCAT 
TTTAAAGAGC TACCTTCAGC TATTATGCCA GGTTCTGAAA AGATCAGGAG ACTAGTTGAA 
GTCTTGAGAA CTGATGTAAT TCGTGGCCTG GGAGTTCAGC TTTTAGAGCA GGTGTATGAT 
CTTTTGGAGG AGGAGGATGA ATTTGATAGA GAGTGTCCCT GTCTGGCCCC AGCACCAGGG 
CATTGTCGTC AGCCTCATAA AATGAACCAG GAATTACCCC TTCCTCCTCA ACTCTCTGGA 
AAAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_011534039.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532341.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu60776
Clone ID Related Accession (Same CDS sequence)	XM_011534039.3 , XM_011534039.2
Accession Version	XM_011534039.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGAAAGG AAACAGATTC ATTGTCTGTC TGAGGATGAG 
TTAAGTTCTT CTACAAGTTC AACTGATAAG TCAGATGGGG ATTACGGGGA AGGGAAAGGT 
CAGACAAATG AAATTAATGC CTTGGTACAA TTGATGACTC AGACCCTGAA ACTGGATTCT 
AAAGAGAGCT GTGAAGATGT CCCGGTAGCA AACCCAGTGT CAGAATTCAA ACTTCATCGG 
AAATATCGGG ACACACTGAT ACTTCATGGG AAGGTTGCAG AAGAGGCAGA GGAAATCCAT 
TTTAAAGAGC TACCTTCAGC TATTATGCCA GGTTCTGAAA AGATCAGGAG ACTAGTTGAA 
GTCTTGAGAA CTGATGTAAT TCGTGGCCTG GGAGTTCAGC TTTTAGAGCA GGTGTATGAT 
CTTTTGGAGG AGGAGGATGA ATTTGATAGA GAGTGTCCCT GTCTGGCCCC AGCACCAGGG 
CATTGTCGTC AGCCTCATAA AATGAACCAG GAATTACCCC TTCCTCCTCA ACTCTCTGGA 
AAAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532341.1
CDS	201..2729
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDRECPCLAPAPGHCRQPHKMNQELPLPPQLSGKV

Target ORF information:

RefSeq Version	XM_011534039.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534039.2

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGAAAGG AAACAGATTC ATTGTCTGTC TGAGGATGAG 
TTAAGTTCTT CTACAAGTTC AACTGATAAG TCAGATGGGG ATTACGGGGA AGGGAAAGGT 
CAGACAAATG AAATTAATGC CTTGGTACAA TTGATGACTC AGACCCTGAA ACTGGATTCT 
AAAGAGAGCT GTGAAGATGT CCCGGTAGCA AACCCAGTGT CAGAATTCAA ACTTCATCGG 
AAATATCGGG ACACACTGAT ACTTCATGGG AAGGTTGCAG AAGAGGCAGA GGAAATCCAT 
TTTAAAGAGC TACCTTCAGC TATTATGCCA GGTTCTGAAA AGATCAGGAG ACTAGTTGAA 
GTCTTGAGAA CTGATGTAAT TCGTGGCCTG GGAGTTCAGC TTTTAGAGCA GGTGTATGAT 
CTTTTGGAGG AGGAGGATGA ATTTGATAGA GAGTGTCCCT GTCTGGCCCC AGCACCAGGG 
CATTGTCGTC AGCCTCATAA AATGAACCAG GAATTACCCC TTCCTCCTCA ACTCTCTGGA 
AAAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_011534040.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532342.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu60777
Clone ID Related Accession (Same CDS sequence)	XM_011534040.2 , XM_011534040.3
Accession Version	XM_011534040.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGTGATC CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532342.1
CDS	201..2216
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQVIL

Target ORF information:

RefSeq Version	XM_011534040.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534040.2

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGTGATC CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_011534040.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532342.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu60777
Clone ID Related Accession (Same CDS sequence)	XM_011534040.2 , XM_011534040.3
Accession Version	XM_011534040.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011534040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGTGATC CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532342.1
CDS	201..2216
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQVIL

Target ORF information:

RefSeq Version	XM_011534040.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534040.3

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGTGATC CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( NM_001348414.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335343.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu102245
Clone ID Related Accession (Same CDS sequence)	NM_001348414.2 , NM_001348414.1
Accession Version	NM_001348414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006254.10 and AC104446.2. On May 31, 2019 this sequence version replaced NM_001348414.1. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (5) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.29463.1, SRR1660803.251755.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTTCT GTGAAGGAGG TGATTTGTAC CGAAAGCTCA AGGAGCAGAA AGGGCAGCTT 
CTGCCTGAGA ATCAGGTGGT AGAGTGGTTT GTACAGATCG CCATGGCTTT GCAGTATTTA 
CATGAAAAAC ACATCCTTCA TCGAGATCTG AAAACTCAAA ATGTCTTCCT AACAAGAACA 
AACATCATCA AAGTAGGGGA CCTAGGAATT GCCCGAGTGT TAGAGAACCA CTGTGACATG 
GCTAGCACCC TCATTGGCAC ACCCTACTAC ATGAGCCCTG AATTGTTCTC AAACAAACCC 
TACAACTATA AGTCTGATGT TTGGGCTCTA GGATGCTGTG TCTATGAAAT GGCCACCTTG 
AAGCATGCTT TCAATGCAAA AGATATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
CTGCCACCAA TGCCAAGAGA TTACAGCCCA GAGCTGGCAG AACTGATAAG AACAATGCTG 
AGCAAAAGGC CTGAAGAAAG GCCGTCTGTG AGGAGCATCC TGAGGCAGCC TTATATAAAG 
CGGCAAATCT CCTTCTTTTT GGAGGCCACA AAGATAAAAA CCTCCAAAAA TAACATTAAA 
AATGGTGACT CTCAATCCAA GCCTTTTGCT ACAGTGGTTT CTGGAGAGGC AGAATCAAAT 
CATGAAGTAA TCCACCCCCA ACCACTCTCT TCTGAGGGCT CCCAGACATA TATAATGGGT 
GAAGGCAAAT GTTTGTCCCA GGAGAAACCC AGGGCCTCTG GTCTCTTGAA GTCACCTGCC 
AGTCTGAAAG CCCATACCTG CAAACAGGAC TTGAGCAATA CCACAGAACT AGCCACAATC 
AGTAGCGTAA ATATTGACAT CTTACCTGCA AAAGGGAGGG ATTCAGTGAG TGATGGCTTT 
GTTCAGGAGA ATCAGCCAAG ATATTTGGAT GCCTCTAATG AGTTAGGAGG TATATGCAGT 
ATTTCTCAAG TGGAAGAGGA GATGCTGCAG GACAACACTA AATCCAGTGC CCAGCCTGAA 
AACCTGATTC CCATGTGGTC CTCTGACATT GTCACTGGGG AAAAGAATGA ACCAGTGAAG 
CCTCTGCAGC CCCTAATCAA AGAACAAAAG CCAAAGGACC AGAGTCTTGC CCTGTCGCCC 
AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACAGCAACC TCCGCCTCCT GGGTTCAAGT 
GATTCTCCAG CCTCAGCCTC CCGAGTAGCT GGGATTACAG GCGTGTGCCA CCACGCCCAG 
GATCAAGTTG CTGGTGAATG TATTATAGAA AAACAGGGCA GAATCCACCC AGATTTACAG 
CCACACAACT CTGGGTCTGA ACCTTCCCTG TCTCGACAGC GACGGCAAAA GAGGAGAGAA 
CAGACTGAGC ACAGAGGGGA AAAGAGACAG GTCCGCAGAG ATCTCTTTGC TTTCCAAGAG 
TCGCCTCCTC GATTTTTGCC TTCTCATCCC ATTGTTGGGA AAGTGGATGT CACATCAACA 
CAAAAAGAGG CTGAAAACCA ACGTAGAGTG GTCACTGGGT CTGTGAGCAG TTCAAGGAGC 
AGTGAGATGT CATCATCAAA GGATCGACCA TTATCAGCCA GAGAGAGGAG GCGACTAAAG 
CAGTCACAGG AAGAAATGTC CTCTTCAGGC CCTTCAGTGA GGAAAGCGTC TCTGAGTGTA 
GCAGGGCCAG GAAAACCCCA GGAAGAAGAC CAGCCCTTGC CTGCCCGACG GCTCTCCTCT 
GACTGCAGCG TCACTCAGGA AAGGAAACAG ATTCATTGTC TGTCTGAGGA TGAGTTAAGT 
TCTTCTACAA GTTCAACTGA TAAGTCAGAT GGGGATTACG GGGAAGGGAA AGGTCAGACA 
AATGAAATTA ATGCCTTGGT ACAATTGATG ACTCAGACCC TGAAACTGGA TTCTAAAGAG 
AGCTGTGAAG ATGTCCCGGT AGCAAACCCA GTGTCAGAAT TCAAACTTCA TCGGAAATAT 
CGGGACACAC TGATACTTCA TGGGAAGGTT GCAGAAGAGG CAGAGGAAAT CCATTTTAAA 
GAGCTACCTT CAGCTATTAT GCCAGGTTCT GAAAAGATCA GGAGACTAGT TGAAGTCTTG 
AGAACTGATG TAATTCGTGG CCTGGGAGTT CAGCTTTTAG AGCAGGTGTA TGATCTTTTG 
GAGGAGGAGG ATGAATTTGA TAGAGAGGTA CGTTTGCGGG AGCACATGGG TGAAAAGTAT 
ACAACTTACA GTGTGAAAGC TCGCCAGTTG AAATTTTTTG AAGAAAACAT GAATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335343.1
CDS	403..2682
Translation	MGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMNF

Target ORF information:

RefSeq Version	NM_001348414.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348414.2

ORF Insert Sequence:

ATGGGCTTCT GTGAAGGAGG TGATTTGTAC CGAAAGCTCA AGGAGCAGAA AGGGCAGCTT 
CTGCCTGAGA ATCAGGTGGT AGAGTGGTTT GTACAGATCG CCATGGCTTT GCAGTATTTA 
CATGAAAAAC ACATCCTTCA TCGAGATCTG AAAACTCAAA ATGTCTTCCT AACAAGAACA 
AACATCATCA AAGTAGGGGA CCTAGGAATT GCCCGAGTGT TAGAGAACCA CTGTGACATG 
GCTAGCACCC TCATTGGCAC ACCCTACTAC ATGAGCCCTG AATTGTTCTC AAACAAACCC 
TACAACTATA AGTCTGATGT TTGGGCTCTA GGATGCTGTG TCTATGAAAT GGCCACCTTG 
AAGCATGCTT TCAATGCAAA AGATATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
CTGCCACCAA TGCCAAGAGA TTACAGCCCA GAGCTGGCAG AACTGATAAG AACAATGCTG 
AGCAAAAGGC CTGAAGAAAG GCCGTCTGTG AGGAGCATCC TGAGGCAGCC TTATATAAAG 
CGGCAAATCT CCTTCTTTTT GGAGGCCACA AAGATAAAAA CCTCCAAAAA TAACATTAAA 
AATGGTGACT CTCAATCCAA GCCTTTTGCT ACAGTGGTTT CTGGAGAGGC AGAATCAAAT 
CATGAAGTAA TCCACCCCCA ACCACTCTCT TCTGAGGGCT CCCAGACATA TATAATGGGT 
GAAGGCAAAT GTTTGTCCCA GGAGAAACCC AGGGCCTCTG GTCTCTTGAA GTCACCTGCC 
AGTCTGAAAG CCCATACCTG CAAACAGGAC TTGAGCAATA CCACAGAACT AGCCACAATC 
AGTAGCGTAA ATATTGACAT CTTACCTGCA AAAGGGAGGG ATTCAGTGAG TGATGGCTTT 
GTTCAGGAGA ATCAGCCAAG ATATTTGGAT GCCTCTAATG AGTTAGGAGG TATATGCAGT 
ATTTCTCAAG TGGAAGAGGA GATGCTGCAG GACAACACTA AATCCAGTGC CCAGCCTGAA 
AACCTGATTC CCATGTGGTC CTCTGACATT GTCACTGGGG AAAAGAATGA ACCAGTGAAG 
CCTCTGCAGC CCCTAATCAA AGAACAAAAG CCAAAGGACC AGAGTCTTGC CCTGTCGCCC 
AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACAGCAACC TCCGCCTCCT GGGTTCAAGT 
GATTCTCCAG CCTCAGCCTC CCGAGTAGCT GGGATTACAG GCGTGTGCCA CCACGCCCAG 
GATCAAGTTG CTGGTGAATG TATTATAGAA AAACAGGGCA GAATCCACCC AGATTTACAG 
CCACACAACT CTGGGTCTGA ACCTTCCCTG TCTCGACAGC GACGGCAAAA GAGGAGAGAA 
CAGACTGAGC ACAGAGGGGA AAAGAGACAG GTCCGCAGAG ATCTCTTTGC TTTCCAAGAG 
TCGCCTCCTC GATTTTTGCC TTCTCATCCC ATTGTTGGGA AAGTGGATGT CACATCAACA 
CAAAAAGAGG CTGAAAACCA ACGTAGAGTG GTCACTGGGT CTGTGAGCAG TTCAAGGAGC 
AGTGAGATGT CATCATCAAA GGATCGACCA TTATCAGCCA GAGAGAGGAG GCGACTAAAG 
CAGTCACAGG AAGAAATGTC CTCTTCAGGC CCTTCAGTGA GGAAAGCGTC TCTGAGTGTA 
GCAGGGCCAG GAAAACCCCA GGAAGAAGAC CAGCCCTTGC CTGCCCGACG GCTCTCCTCT 
GACTGCAGCG TCACTCAGGA AAGGAAACAG ATTCATTGTC TGTCTGAGGA TGAGTTAAGT 
TCTTCTACAA GTTCAACTGA TAAGTCAGAT GGGGATTACG GGGAAGGGAA AGGTCAGACA 
AATGAAATTA ATGCCTTGGT ACAATTGATG ACTCAGACCC TGAAACTGGA TTCTAAAGAG 
AGCTGTGAAG ATGTCCCGGT AGCAAACCCA GTGTCAGAAT TCAAACTTCA TCGGAAATAT 
CGGGACACAC TGATACTTCA TGGGAAGGTT GCAGAAGAGG CAGAGGAAAT CCATTTTAAA 
GAGCTACCTT CAGCTATTAT GCCAGGTTCT GAAAAGATCA GGAGACTAGT TGAAGTCTTG 
AGAACTGATG TAATTCGTGG CCTGGGAGTT CAGCTTTTAG AGCAGGTGTA TGATCTTTTG 
GAGGAGGAGG ATGAATTTGA TAGAGAGGTA CGTTTGCGGG AGCACATGGG TGAAAAGTAT 
ACAACTTACA GTGTGAAAGC TCGCCAGTTG AAATTTTTTG AAGAAAACAT GAATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( NM_001348414.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335343.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu102245
Clone ID Related Accession (Same CDS sequence)	NM_001348414.2 , NM_001348414.1
Accession Version	NM_001348414.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-28
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006254.10 and AC104446.2. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (5) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.29463.1, SRR1660803.251755.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTTCT GTGAAGGAGG TGATTTGTAC CGAAAGCTCA AGGAGCAGAA AGGGCAGCTT 
CTGCCTGAGA ATCAGGTGGT AGAGTGGTTT GTACAGATCG CCATGGCTTT GCAGTATTTA 
CATGAAAAAC ACATCCTTCA TCGAGATCTG AAAACTCAAA ATGTCTTCCT AACAAGAACA 
AACATCATCA AAGTAGGGGA CCTAGGAATT GCCCGAGTGT TAGAGAACCA CTGTGACATG 
GCTAGCACCC TCATTGGCAC ACCCTACTAC ATGAGCCCTG AATTGTTCTC AAACAAACCC 
TACAACTATA AGTCTGATGT TTGGGCTCTA GGATGCTGTG TCTATGAAAT GGCCACCTTG 
AAGCATGCTT TCAATGCAAA AGATATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
CTGCCACCAA TGCCAAGAGA TTACAGCCCA GAGCTGGCAG AACTGATAAG AACAATGCTG 
AGCAAAAGGC CTGAAGAAAG GCCGTCTGTG AGGAGCATCC TGAGGCAGCC TTATATAAAG 
CGGCAAATCT CCTTCTTTTT GGAGGCCACA AAGATAAAAA CCTCCAAAAA TAACATTAAA 
AATGGTGACT CTCAATCCAA GCCTTTTGCT ACAGTGGTTT CTGGAGAGGC AGAATCAAAT 
CATGAAGTAA TCCACCCCCA ACCACTCTCT TCTGAGGGCT CCCAGACATA TATAATGGGT 
GAAGGCAAAT GTTTGTCCCA GGAGAAACCC AGGGCCTCTG GTCTCTTGAA GTCACCTGCC 
AGTCTGAAAG CCCATACCTG CAAACAGGAC TTGAGCAATA CCACAGAACT AGCCACAATC 
AGTAGCGTAA ATATTGACAT CTTACCTGCA AAAGGGAGGG ATTCAGTGAG TGATGGCTTT 
GTTCAGGAGA ATCAGCCAAG ATATTTGGAT GCCTCTAATG AGTTAGGAGG TATATGCAGT 
ATTTCTCAAG TGGAAGAGGA GATGCTGCAG GACAACACTA AATCCAGTGC CCAGCCTGAA 
AACCTGATTC CCATGTGGTC CTCTGACATT GTCACTGGGG AAAAGAATGA ACCAGTGAAG 
CCTCTGCAGC CCCTAATCAA AGAACAAAAG CCAAAGGACC AGAGTCTTGC CCTGTCGCCC 
AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACAGCAACC TCCGCCTCCT GGGTTCAAGT 
GATTCTCCAG CCTCAGCCTC CCGAGTAGCT GGGATTACAG GCGTGTGCCA CCACGCCCAG 
GATCAAGTTG CTGGTGAATG TATTATAGAA AAACAGGGCA GAATCCACCC AGATTTACAG 
CCACACAACT CTGGGTCTGA ACCTTCCCTG TCTCGACAGC GACGGCAAAA GAGGAGAGAA 
CAGACTGAGC ACAGAGGGGA AAAGAGACAG GTCCGCAGAG ATCTCTTTGC TTTCCAAGAG 
TCGCCTCCTC GATTTTTGCC TTCTCATCCC ATTGTTGGGA AAGTGGATGT CACATCAACA 
CAAAAAGAGG CTGAAAACCA ACGTAGAGTG GTCACTGGGT CTGTGAGCAG TTCAAGGAGC 
AGTGAGATGT CATCATCAAA GGATCGACCA TTATCAGCCA GAGAGAGGAG GCGACTAAAG 
CAGTCACAGG AAGAAATGTC CTCTTCAGGC CCTTCAGTGA GGAAAGCGTC TCTGAGTGTA 
GCAGGGCCAG GAAAACCCCA GGAAGAAGAC CAGCCCTTGC CTGCCCGACG GCTCTCCTCT 
GACTGCAGCG TCACTCAGGA AAGGAAACAG ATTCATTGTC TGTCTGAGGA TGAGTTAAGT 
TCTTCTACAA GTTCAACTGA TAAGTCAGAT GGGGATTACG GGGAAGGGAA AGGTCAGACA 
AATGAAATTA ATGCCTTGGT ACAATTGATG ACTCAGACCC TGAAACTGGA TTCTAAAGAG 
AGCTGTGAAG ATGTCCCGGT AGCAAACCCA GTGTCAGAAT TCAAACTTCA TCGGAAATAT 
CGGGACACAC TGATACTTCA TGGGAAGGTT GCAGAAGAGG CAGAGGAAAT CCATTTTAAA 
GAGCTACCTT CAGCTATTAT GCCAGGTTCT GAAAAGATCA GGAGACTAGT TGAAGTCTTG 
AGAACTGATG TAATTCGTGG CCTGGGAGTT CAGCTTTTAG AGCAGGTGTA TGATCTTTTG 
GAGGAGGAGG ATGAATTTGA TAGAGAGGTA CGTTTGCGGG AGCACATGGG TGAAAAGTAT 
ACAACTTACA GTGTGAAAGC TCGCCAGTTG AAATTTTTTG AAGAAAACAT GAATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335343.1
CDS	412..2691
Translation	MGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMNF

Target ORF information:

RefSeq Version	NM_001348414.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348414.1

ORF Insert Sequence:

ATGGGCTTCT GTGAAGGAGG TGATTTGTAC CGAAAGCTCA AGGAGCAGAA AGGGCAGCTT 
CTGCCTGAGA ATCAGGTGGT AGAGTGGTTT GTACAGATCG CCATGGCTTT GCAGTATTTA 
CATGAAAAAC ACATCCTTCA TCGAGATCTG AAAACTCAAA ATGTCTTCCT AACAAGAACA 
AACATCATCA AAGTAGGGGA CCTAGGAATT GCCCGAGTGT TAGAGAACCA CTGTGACATG 
GCTAGCACCC TCATTGGCAC ACCCTACTAC ATGAGCCCTG AATTGTTCTC AAACAAACCC 
TACAACTATA AGTCTGATGT TTGGGCTCTA GGATGCTGTG TCTATGAAAT GGCCACCTTG 
AAGCATGCTT TCAATGCAAA AGATATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
CTGCCACCAA TGCCAAGAGA TTACAGCCCA GAGCTGGCAG AACTGATAAG AACAATGCTG 
AGCAAAAGGC CTGAAGAAAG GCCGTCTGTG AGGAGCATCC TGAGGCAGCC TTATATAAAG 
CGGCAAATCT CCTTCTTTTT GGAGGCCACA AAGATAAAAA CCTCCAAAAA TAACATTAAA 
AATGGTGACT CTCAATCCAA GCCTTTTGCT ACAGTGGTTT CTGGAGAGGC AGAATCAAAT 
CATGAAGTAA TCCACCCCCA ACCACTCTCT TCTGAGGGCT CCCAGACATA TATAATGGGT 
GAAGGCAAAT GTTTGTCCCA GGAGAAACCC AGGGCCTCTG GTCTCTTGAA GTCACCTGCC 
AGTCTGAAAG CCCATACCTG CAAACAGGAC TTGAGCAATA CCACAGAACT AGCCACAATC 
AGTAGCGTAA ATATTGACAT CTTACCTGCA AAAGGGAGGG ATTCAGTGAG TGATGGCTTT 
GTTCAGGAGA ATCAGCCAAG ATATTTGGAT GCCTCTAATG AGTTAGGAGG TATATGCAGT 
ATTTCTCAAG TGGAAGAGGA GATGCTGCAG GACAACACTA AATCCAGTGC CCAGCCTGAA 
AACCTGATTC CCATGTGGTC CTCTGACATT GTCACTGGGG AAAAGAATGA ACCAGTGAAG 
CCTCTGCAGC CCCTAATCAA AGAACAAAAG CCAAAGGACC AGAGTCTTGC CCTGTCGCCC 
AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACAGCAACC TCCGCCTCCT GGGTTCAAGT 
GATTCTCCAG CCTCAGCCTC CCGAGTAGCT GGGATTACAG GCGTGTGCCA CCACGCCCAG 
GATCAAGTTG CTGGTGAATG TATTATAGAA AAACAGGGCA GAATCCACCC AGATTTACAG 
CCACACAACT CTGGGTCTGA ACCTTCCCTG TCTCGACAGC GACGGCAAAA GAGGAGAGAA 
CAGACTGAGC ACAGAGGGGA AAAGAGACAG GTCCGCAGAG ATCTCTTTGC TTTCCAAGAG 
TCGCCTCCTC GATTTTTGCC TTCTCATCCC ATTGTTGGGA AAGTGGATGT CACATCAACA 
CAAAAAGAGG CTGAAAACCA ACGTAGAGTG GTCACTGGGT CTGTGAGCAG TTCAAGGAGC 
AGTGAGATGT CATCATCAAA GGATCGACCA TTATCAGCCA GAGAGAGGAG GCGACTAAAG 
CAGTCACAGG AAGAAATGTC CTCTTCAGGC CCTTCAGTGA GGAAAGCGTC TCTGAGTGTA 
GCAGGGCCAG GAAAACCCCA GGAAGAAGAC CAGCCCTTGC CTGCCCGACG GCTCTCCTCT 
GACTGCAGCG TCACTCAGGA AAGGAAACAG ATTCATTGTC TGTCTGAGGA TGAGTTAAGT 
TCTTCTACAA GTTCAACTGA TAAGTCAGAT GGGGATTACG GGGAAGGGAA AGGTCAGACA 
AATGAAATTA ATGCCTTGGT ACAATTGATG ACTCAGACCC TGAAACTGGA TTCTAAAGAG 
AGCTGTGAAG ATGTCCCGGT AGCAAACCCA GTGTCAGAAT TCAAACTTCA TCGGAAATAT 
CGGGACACAC TGATACTTCA TGGGAAGGTT GCAGAAGAGG CAGAGGAAAT CCATTTTAAA 
GAGCTACCTT CAGCTATTAT GCCAGGTTCT GAAAAGATCA GGAGACTAGT TGAAGTCTTG 
AGAACTGATG TAATTCGTGG CCTGGGAGTT CAGCTTTTAG AGCAGGTGTA TGATCTTTTG 
GAGGAGGAGG ATGAATTTGA TAGAGAGGTA CGTTTGCGGG AGCACATGGG TGAAAAGTAT 
ACAACTTACA GTGTGAAAGC TCGCCAGTTG AAATTTTTTG AAGAAAACAT GAATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( NM_001348413.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335342.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu102374
Clone ID Related Accession (Same CDS sequence)	NM_001348413.2 , NM_001348413.1
Accession Version	NM_001348413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006254.10. On Jun 1, 2019 this sequence version replaced NM_001348413.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon and differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (4) lacks an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063044.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGGA TCAAGTTGCT 
GGTGAATGTA TTATAGAAAA ACAGGGCAGA ATCCACCCAG ATTTACAGCC ACACAACTCT 
GGGTCTGAAC CTTCCCTGTC TCGACAGCGA CGGCAAAAGA GGAGAGAACA GACTGAGCAC 
AGAGGGGAAA AGAGACAGGT CCGCAGAGAT CTCTTTGCTT TCCAAGAGTC GCCTCCTCGA 
TTTTTGCCTT CTCATCCCAT TGTTGGGAAA GTGGATGTCA CATCAACACA AAAAGAGGCT 
GAAAACCAAC GTAGAGTGGT CACTGGGTCT GTGAGCAGTT CAAGGAGCAG TGAGATGTCA 
TCATCAAAGG ATCGACCATT ATCAGCCAGA GAGAGGAGGC GACTAAAGCA GTCACAGGAA 
GAAATGTCCT CTTCAGGCCC TTCAGTGAGG AAAGCGTCTC TGAGTGTAGC AGGGCCAGGA 
AAACCCCAGG AAGAAGACCA GCCCTTGCCT GCCCGACGGC TCTCCTCTGA CTGCAGCGTC 
ACTCAGGAAA GGAAACAGAT TCATTGTCTG TCTGAGGATG AGTTAAGTTC TTCTACAAGT 
TCAACTGATA AGTCAGATGG GGATTACGGG GAAGGGAAAG GTCAGACAAA TGAAATTAAT 
GCCTTGGTAC AATTGATGAC TCAGACCCTG AAACTGGATT CTAAAGAGAG CTGTGAAGAT 
GTCCCGGTAG CAAACCCAGT GTCAGAATTC AAACTTCATC GGAAATATCG GGACACACTG 
ATACTTCATG GGAAGGTTGC AGAAGAGGCA GAGGAAATCC ATTTTAAAGA GCTACCTTCA 
GCTATTATGC CAGGTTCTGA AAAGATCAGG AGACTAGTTG AAGTCTTGAG AACTGATGTA 
ATTCGTGGCC TGGGAGTTCA GCTTTTAGAG CAGGTGTATG ATCTTTTGGA GGAGGAGGAT 
GAATTTGATA GAGAGGTGAG TGTCTCATTA ACTGTATCAA GATGCTTATG TTATAGAATT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335342.1
CDS	195..2540
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVSVSLTVSRCLCYRIF

Target ORF information:

RefSeq Version	NM_001348413.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348413.2

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGGA TCAAGTTGCT 
GGTGAATGTA TTATAGAAAA ACAGGGCAGA ATCCACCCAG ATTTACAGCC ACACAACTCT 
GGGTCTGAAC CTTCCCTGTC TCGACAGCGA CGGCAAAAGA GGAGAGAACA GACTGAGCAC 
AGAGGGGAAA AGAGACAGGT CCGCAGAGAT CTCTTTGCTT TCCAAGAGTC GCCTCCTCGA 
TTTTTGCCTT CTCATCCCAT TGTTGGGAAA GTGGATGTCA CATCAACACA AAAAGAGGCT 
GAAAACCAAC GTAGAGTGGT CACTGGGTCT GTGAGCAGTT CAAGGAGCAG TGAGATGTCA 
TCATCAAAGG ATCGACCATT ATCAGCCAGA GAGAGGAGGC GACTAAAGCA GTCACAGGAA 
GAAATGTCCT CTTCAGGCCC TTCAGTGAGG AAAGCGTCTC TGAGTGTAGC AGGGCCAGGA 
AAACCCCAGG AAGAAGACCA GCCCTTGCCT GCCCGACGGC TCTCCTCTGA CTGCAGCGTC 
ACTCAGGAAA GGAAACAGAT TCATTGTCTG TCTGAGGATG AGTTAAGTTC TTCTACAAGT 
TCAACTGATA AGTCAGATGG GGATTACGGG GAAGGGAAAG GTCAGACAAA TGAAATTAAT 
GCCTTGGTAC AATTGATGAC TCAGACCCTG AAACTGGATT CTAAAGAGAG CTGTGAAGAT 
GTCCCGGTAG CAAACCCAGT GTCAGAATTC AAACTTCATC GGAAATATCG GGACACACTG 
ATACTTCATG GGAAGGTTGC AGAAGAGGCA GAGGAAATCC ATTTTAAAGA GCTACCTTCA 
GCTATTATGC CAGGTTCTGA AAAGATCAGG AGACTAGTTG AAGTCTTGAG AACTGATGTA 
ATTCGTGGCC TGGGAGTTCA GCTTTTAGAG CAGGTGTATG ATCTTTTGGA GGAGGAGGAT 
GAATTTGATA GAGAGGTGAG TGTCTCATTA ACTGTATCAA GATGCTTATG TTATAGAATT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( NM_001348413.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335342.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu102374
Clone ID Related Accession (Same CDS sequence)	NM_001348413.2 , NM_001348413.1
Accession Version	NM_001348413.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-28
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006254.10. Transcript Variant: This variant (4) lacks an alternate in-frame exon and differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (4) lacks an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC063044.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGGA TCAAGTTGCT 
GGTGAATGTA TTATAGAAAA ACAGGGCAGA ATCCACCCAG ATTTACAGCC ACACAACTCT 
GGGTCTGAAC CTTCCCTGTC TCGACAGCGA CGGCAAAAGA GGAGAGAACA GACTGAGCAC 
AGAGGGGAAA AGAGACAGGT CCGCAGAGAT CTCTTTGCTT TCCAAGAGTC GCCTCCTCGA 
TTTTTGCCTT CTCATCCCAT TGTTGGGAAA GTGGATGTCA CATCAACACA AAAAGAGGCT 
GAAAACCAAC GTAGAGTGGT CACTGGGTCT GTGAGCAGTT CAAGGAGCAG TGAGATGTCA 
TCATCAAAGG ATCGACCATT ATCAGCCAGA GAGAGGAGGC GACTAAAGCA GTCACAGGAA 
GAAATGTCCT CTTCAGGCCC TTCAGTGAGG AAAGCGTCTC TGAGTGTAGC AGGGCCAGGA 
AAACCCCAGG AAGAAGACCA GCCCTTGCCT GCCCGACGGC TCTCCTCTGA CTGCAGCGTC 
ACTCAGGAAA GGAAACAGAT TCATTGTCTG TCTGAGGATG AGTTAAGTTC TTCTACAAGT 
TCAACTGATA AGTCAGATGG GGATTACGGG GAAGGGAAAG GTCAGACAAA TGAAATTAAT 
GCCTTGGTAC AATTGATGAC TCAGACCCTG AAACTGGATT CTAAAGAGAG CTGTGAAGAT 
GTCCCGGTAG CAAACCCAGT GTCAGAATTC AAACTTCATC GGAAATATCG GGACACACTG 
ATACTTCATG GGAAGGTTGC AGAAGAGGCA GAGGAAATCC ATTTTAAAGA GCTACCTTCA 
GCTATTATGC CAGGTTCTGA AAAGATCAGG AGACTAGTTG AAGTCTTGAG AACTGATGTA 
ATTCGTGGCC TGGGAGTTCA GCTTTTAGAG CAGGTGTATG ATCTTTTGGA GGAGGAGGAT 
GAATTTGATA GAGAGGTGAG TGTCTCATTA ACTGTATCAA GATGCTTATG TTATAGAATT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335342.1
CDS	204..2549
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVSVSLTVSRCLCYRIF

Target ORF information:

RefSeq Version	NM_001348413.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348413.1

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGGA TCAAGTTGCT 
GGTGAATGTA TTATAGAAAA ACAGGGCAGA ATCCACCCAG ATTTACAGCC ACACAACTCT 
GGGTCTGAAC CTTCCCTGTC TCGACAGCGA CGGCAAAAGA GGAGAGAACA GACTGAGCAC 
AGAGGGGAAA AGAGACAGGT CCGCAGAGAT CTCTTTGCTT TCCAAGAGTC GCCTCCTCGA 
TTTTTGCCTT CTCATCCCAT TGTTGGGAAA GTGGATGTCA CATCAACACA AAAAGAGGCT 
GAAAACCAAC GTAGAGTGGT CACTGGGTCT GTGAGCAGTT CAAGGAGCAG TGAGATGTCA 
TCATCAAAGG ATCGACCATT ATCAGCCAGA GAGAGGAGGC GACTAAAGCA GTCACAGGAA 
GAAATGTCCT CTTCAGGCCC TTCAGTGAGG AAAGCGTCTC TGAGTGTAGC AGGGCCAGGA 
AAACCCCAGG AAGAAGACCA GCCCTTGCCT GCCCGACGGC TCTCCTCTGA CTGCAGCGTC 
ACTCAGGAAA GGAAACAGAT TCATTGTCTG TCTGAGGATG AGTTAAGTTC TTCTACAAGT 
TCAACTGATA AGTCAGATGG GGATTACGGG GAAGGGAAAG GTCAGACAAA TGAAATTAAT 
GCCTTGGTAC AATTGATGAC TCAGACCCTG AAACTGGATT CTAAAGAGAG CTGTGAAGAT 
GTCCCGGTAG CAAACCCAGT GTCAGAATTC AAACTTCATC GGAAATATCG GGACACACTG 
ATACTTCATG GGAAGGTTGC AGAAGAGGCA GAGGAAATCC ATTTTAAAGA GCTACCTTCA 
GCTATTATGC CAGGTTCTGA AAAGATCAGG AGACTAGTTG AAGTCTTGAG AACTGATGTA 
ATTCGTGGCC TGGGAGTTCA GCTTTTAGAG CAGGTGTATG ATCTTTTGGA GGAGGAGGAT 
GAATTTGATA GAGAGGTGAG TGTCTCATTA ACTGTATCAA GATGCTTATG TTATAGAATT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_017007085.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862574.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu94523
Clone ID Related Accession (Same CDS sequence)	XM_017007085.1
Accession Version	XM_017007085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGAAAGG AAACAGATTC ATTGTCTGTC TGAGGATGAG 
TTAAGTTCTT CTACAAGTTC AACTGATAAG TCAGATGGGG ATTACGGGGA AGGGAAAGGT 
CAGACAAATG AAATTAATGC CTTGGTACAA TTGATGACTC AGACCCTGAA ACTGGATTCT 
AAAGAGAGCT GTGAAGATGT CCCGGTAGCA AACCCAGTGT CAGAATTCAA ACTTCATCGG 
AAATATCGGG ACACACTGAT ACTTCATGGG AAGGTTGCAG AAGAGGCAGA GGAAATCCAT 
TTTAAAGAGC TACCTTCAGC TATTATGCCA GGTTCTGAAA AGATCAGGAG ACTAGTTGAA 
GTCTTGAGAA CTGATGTAAT TCGTGGCCTG GGAGTTCAGC TTTTAGAGCA GGTGTATGAT 
CTTTTGGAGG AGGAGGATGA ATTTGATAGA GAGCTTCTCC CTGTGTTCAC ACCTTTGATG 
CATTCCCTGC CATCTGGATT GTTTGCTATC TTCACCCTGC AGAGTCCAGA GCTCTCGCTT 
TGTCTCCAAC ATGGAGATAA TGCTCAGGTC AGGAAGACAG ATTCCCAGAA AGACCAGGTT 
CCTGTAGTTC TCCTACATCA CTTCCCTGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862574.1
CDS	201..2813
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDRELLPVFTPLMHSLPSGLFAIFTLQSPELSLCLQHGDNAQVRKTDSQKDQVPVVLLHHFPV

Target ORF information:

RefSeq Version	XM_017007085.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007085.1

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGAG TCTTGCCCTG 
TCGCCCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACA GCAACCTCCG CCTCCTGGGT 
TCAAGTGATT CTCCAGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGCGT GTGCCACCAC 
GCCCAGGATC AAGTTGCTGG TGAATGTATT ATAGAAAAAC AGGGCAGAAT CCACCCAGAT 
TTACAGCCAC ACAACTCTGG GTCTGAACCT TCCCTGTCTC GACAGCGACG GCAAAAGAGG 
AGAGAACAGA CTGAGCACAG AGGGGAAAAG AGACAGGTCC GCAGAGATCT CTTTGCTTTC 
CAAGAGTCGC CTCCTCGATT TTTGCCTTCT CATCCCATTG TTGGGAAAGT GGATGTCACA 
TCAACACAAA AAGAGGCTGA AAACCAACGT AGAGTGGTCA CTGGGTCTGT GAGCAGTTCA 
AGGAGCAGTG AGATGTCATC ATCAAAGGAT CGACCATTAT CAGCCAGAGA GAGGAGGCGA 
CTAAAGCAGT CACAGGAAGA AATGTCCTCT TCAGGCCCTT CAGTGAGGAA AGCGTCTCTG 
AGTGTAGCAG GGCCAGGAAA ACCCCAGGAA GAAGACCAGC CCTTGCCTGC CCGACGGCTC 
TCCTCTGACT GCAGCGTCAC TCAGGAAAGG AAACAGATTC ATTGTCTGTC TGAGGATGAG 
TTAAGTTCTT CTACAAGTTC AACTGATAAG TCAGATGGGG ATTACGGGGA AGGGAAAGGT 
CAGACAAATG AAATTAATGC CTTGGTACAA TTGATGACTC AGACCCTGAA ACTGGATTCT 
AAAGAGAGCT GTGAAGATGT CCCGGTAGCA AACCCAGTGT CAGAATTCAA ACTTCATCGG 
AAATATCGGG ACACACTGAT ACTTCATGGG AAGGTTGCAG AAGAGGCAGA GGAAATCCAT 
TTTAAAGAGC TACCTTCAGC TATTATGCCA GGTTCTGAAA AGATCAGGAG ACTAGTTGAA 
GTCTTGAGAA CTGATGTAAT TCGTGGCCTG GGAGTTCAGC TTTTAGAGCA GGTGTATGAT 
CTTTTGGAGG AGGAGGATGA ATTTGATAGA GAGCTTCTCC CTGTGTTCAC ACCTTTGATG 
CATTCCCTGC CATCTGGATT GTTTGCTATC TTCACCCTGC AGAGTCCAGA GCTCTCGCTT 
TGTCTCCAAC ATGGAGATAA TGCTCAGGTC AGGAAGACAG ATTCCCAGAA AGACCAGGTT 
CCTGTAGTTC TCCTACATCA CTTCCCTGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK4 ( XM_017007086.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862575.1 Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X3, mRNA.

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CloneID	OHu94524
Clone ID Related Accession (Same CDS sequence)	XM_017007086.1
Accession Version	XM_017007086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGGA TCAAGTTGCT 
GGTGAATGTA TTATAGAAAA ACAGGGCAGA ATCCACCCAG ATTTACAGCC ACACAACTCT 
GGGTCTGAAC CTTCCCTGTC TCGACAGCGA CGGCAAAAGA GGAGAGAACA GACTGAGCAC 
AGAGGGGAAA AGAGACAGGT CCGCAGAGAT CTCTTTGCTT TCCAAGAGTC GCCTCCTCGA 
TTTTTGCCTT CTCATCCCAT TGTTGGGAAA GTGGATGTCA CATCAACACA AAAAGAGGCT 
GAAAACCAAC GTAGAGTGGT CACTGGGTCT GTGAGCAGTT CAAGGAGCAG TGAGATGTCA 
TCATCAAAGG ATCGACCATT ATCAGCCAGA GAGAGGAGGC GACTAAAGCA GTCACAGGAA 
GAAATGTCCT CTTCAGGCCC TTCAGTGAGG AAAGCGTCTC TGAGTGTAGC AGGGCCAGGA 
AAACCCCAGG AAGAAGACCA GCCCTTGCCT GCCCGACGGC TCTCCTCTGA CTGCAGCGTC 
ACTCAGGAAA GGAAACAGAT TCATTGTCTG TCTGAGGATG AGTTAAGTTC TTCTACAAGT 
TCAACTGATA AGTCAGATGG GGATTACGGG GAAGGGAAAG GTCAGACAAA TGAAATTAAT 
GCCTTGGTAC AATTGATGAC TCAGACCCTG AAACTGGATT CTAAAGAGAG CTGTGAAGAT 
GTCCCGGTAG CAAACCCAGT GTCAGAATTC AAACTTCATC GGAAATATCG GGACACACTG 
ATACTTCATG GGAAGGTTGC AGAAGAGGCA GAGGAAATCC ATTTTAAAGA GCTACCTTCA 
GCTATTATGC CAGGTTCTGA AAAGATCAGG AGACTAGTTG AAGTCTTGAG AACTGATGTA 
ATTCGTGGCC TGGGAGTTCA GCTTTTAGAG CAGGTGTATG ATCTTTTGGA GGAGGAGGAT 
GAATTTGATA GAGAGCTTCT CCCTGTGTTC ACACCTTTGA TGCATTCCCT GCCATCTGGA 
TTGTTTGCTA TCTTCACCCT GCAGAGTCCA GAGCTCTCGC TTTGTCTCCA ACATGGAGAT 
AATGCTCAGG TCAGGAAGAC AGATTCCCAG AAAGACCAGG TTCCTGTAGT TCTCCTACAT 
CACTTCCCTG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862575.1
CDS	201..2675
Translation	MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDRELLPVFTPLMHSLPSGLFAIFTLQSPELSLCLQHGDNAQVRKTDSQKDQVPVVLLHHFPV

Target ORF information:

RefSeq Version	XM_017007086.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 4 (NEK4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007086.1

ORF Insert Sequence:

ATGCCCCTGG CCGCCTACTG CTACCTGCGG GTCGTGGGCA AGGGGAGCTA TGGAGAGGTG 
ACGCTTGTGA AGCACCGGCG GGACGGCAAG CAGTATGTCA TCAAAAAACT GAACCTCCGA 
AATGCCTCTA GCCGAGAGCG GCGAGCTGCT GAACAGGAAG CCCAGCTCTT GTCTCAGTTG 
AAGCATCCCA ACATTGTCAC CTACAAGGAG TCATGGGAAG GAGGAGATGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGTGA AGGAGGTGAT TTGTACCGAA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAATCA GGTGGTAGAG TGGTTTGTAC AGATCGCCAT GGCTTTGCAG 
TATTTACATG AAAAACACAT CCTTCATCGA GATCTGAAAA CTCAAAATGT CTTCCTAACA 
AGAACAAACA TCATCAAAGT AGGGGACCTA GGAATTGCCC GAGTGTTAGA GAACCACTGT 
GACATGGCTA GCACCCTCAT TGGCACACCC TACTACATGA GCCCTGAATT GTTCTCAAAC 
AAACCCTACA ACTATAAGTC TGATGTTTGG GCTCTAGGAT GCTGTGTCTA TGAAATGGCC 
ACCTTGAAGC ATGCTTTCAA TGCAAAAGAT ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGCTGC CACCAATGCC AAGAGATTAC AGCCCAGAGC TGGCAGAACT GATAAGAACA 
ATGCTGAGCA AAAGGCCTGA AGAAAGGCCG TCTGTGAGGA GCATCCTGAG GCAGCCTTAT 
ATAAAGCGGC AAATCTCCTT CTTTTTGGAG GCCACAAAGA TAAAAACCTC CAAAAATAAC 
ATTAAAAATG GTGACTCTCA ATCCAAGCCT TTTGCTACAG TGGTTTCTGG AGAGGCAGAA 
TCAAATCATG AAGTAATCCA CCCCCAACCA CTCTCTTCTG AGGGCTCCCA GACATATATA 
ATGGGTGAAG GCAAATGTTT GTCCCAGGAG AAACCCAGGG CCTCTGGTCT CTTGAAGTCA 
CCTGCCAGTC TGAAAGCCCA TACCTGCAAA CAGGACTTGA GCAATACCAC AGAACTAGCC 
ACAATCAGTA GCGTAAATAT TGACATCTTA CCTGCAAAAG GGAGGGATTC AGTGAGTGAT 
GGCTTTGTTC AGGAGAATCA GCCAAGATAT TTGGATGCCT CTAATGAGTT AGGAGGTATA 
TGCAGTATTT CTCAAGTGGA AGAGGAGATG CTGCAGGACA ACACTAAATC CAGTGCCCAG 
CCTGAAAACC TGATTCCCAT GTGGTCCTCT GACATTGTCA CTGGGGAAAA GAATGAACCA 
GTGAAGCCTC TGCAGCCCCT AATCAAAGAA CAAAAGCCAA AGGACCAGGA TCAAGTTGCT 
GGTGAATGTA TTATAGAAAA ACAGGGCAGA ATCCACCCAG ATTTACAGCC ACACAACTCT 
GGGTCTGAAC CTTCCCTGTC TCGACAGCGA CGGCAAAAGA GGAGAGAACA GACTGAGCAC 
AGAGGGGAAA AGAGACAGGT CCGCAGAGAT CTCTTTGCTT TCCAAGAGTC GCCTCCTCGA 
TTTTTGCCTT CTCATCCCAT TGTTGGGAAA GTGGATGTCA CATCAACACA AAAAGAGGCT 
GAAAACCAAC GTAGAGTGGT CACTGGGTCT GTGAGCAGTT CAAGGAGCAG TGAGATGTCA 
TCATCAAAGG ATCGACCATT ATCAGCCAGA GAGAGGAGGC GACTAAAGCA GTCACAGGAA 
GAAATGTCCT CTTCAGGCCC TTCAGTGAGG AAAGCGTCTC TGAGTGTAGC AGGGCCAGGA 
AAACCCCAGG AAGAAGACCA GCCCTTGCCT GCCCGACGGC TCTCCTCTGA CTGCAGCGTC 
ACTCAGGAAA GGAAACAGAT TCATTGTCTG TCTGAGGATG AGTTAAGTTC TTCTACAAGT 
TCAACTGATA AGTCAGATGG GGATTACGGG GAAGGGAAAG GTCAGACAAA TGAAATTAAT 
GCCTTGGTAC AATTGATGAC TCAGACCCTG AAACTGGATT CTAAAGAGAG CTGTGAAGAT 
GTCCCGGTAG CAAACCCAGT GTCAGAATTC AAACTTCATC GGAAATATCG GGACACACTG 
ATACTTCATG GGAAGGTTGC AGAAGAGGCA GAGGAAATCC ATTTTAAAGA GCTACCTTCA 
GCTATTATGC CAGGTTCTGA AAAGATCAGG AGACTAGTTG AAGTCTTGAG AACTGATGTA 
ATTCGTGGCC TGGGAGTTCA GCTTTTAGAG CAGGTGTATG ATCTTTTGGA GGAGGAGGAT 
GAATTTGATA GAGAGCTTCT CCCTGTGTTC ACACCTTTGA TGCATTCCCT GCCATCTGGA 
TTGTTTGCTA TCTTCACCCT GCAGAGTCCA GAGCTCTCGC TTTGTCTCCA ACATGGAGAT 
AATGCTCAGG TCAGGAAGAC AGATTCCCAG AAAGACCAGG TTCCTGTAGT TCTCCTACAT 
CACTTCCCTG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEK4
Entrez Gene ID	6787
Full Name	NIMA related kinase 4
Synonyms	NRK2,STK2,pp12301
General protein information	Preferred Names NIMA related kinase 4 Names serine/threonine-protein kinase Nek4 NIMA (never in mitosis gene a)-related kinase 4 never in mitosis A-related kinase 4 nimA-related protein kinase 4 serine/threonine kinase 2 serine/threonine protein kinase-2 serine/threonine-protein kinase NRK2 serine/threonine-protein kinase Nek4 variant 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.1
Summary	The protein encoded by this gene is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies. [provided by RefSeq, Jan 2017].
Disorder MIM:	601959

Gallus gallus (chicken)	NEK4	XP_414252.2
Danio rerio (zebrafish)	nek4	NP_957306.1
Xenopus tropicalis (tropical clawed frog)	nek4	NP_001072768.1
Homo sapiens (human)	NEK4	NP_003148.2
Pan troglodytes (chimpanzee)	NEK4	XP_516519.4
Canis lupus familiaris (dog)	NEK4	XP_005632492.1
Bos taurus (cattle)	NEK4	NP_001095414.1
Mus musculus (house mouse)	Nek4	NP_035979.1
Rattus norvegicus (Norway rat)	Nek4	NP_001013152.2
	NEK4	XP_002802834.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEK4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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