SRPK1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SRPK1
Entrez Gene ID	6732
Full Name	SRSF protein kinase 1
Synonyms	SFRSK1
General protein information	Preferred Names SRSF protein kinase 1 Names SRSF protein kinase 1 SFRS protein kinase 1 SR-protein-specific kinase 1 serine/arginine-rich splicing factor kinase 1 serine/threonine-protein kinase SRPK1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.31
Summary	This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. Alternative splicing of this gene results in multiple transcript variants. Additional alternatively spliced transcript variants have been described for this gene, but their full length nature have not been determined.[provided by RefSeq, Jul 2010].
Disorder MIM:	601939

mRNA and Protein(s)

mRNA	Protein	Name
NM_003137.4	NP_003128.3	SRSF protein kinase 1
NM_003137.5	NP_003128.3	SRSF protein kinase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SRPK1	XP_001172595.2
Bos taurus (cattle)	SRPK1	XP_002697249.2
Rattus norvegicus (Norway rat)	Srpk1	NP_001020897.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005322	XP_315336.4
Oryza sativa (rice)	Os03g0748400	NP_001051268.2
Gallus gallus (chicken)	SRPK1	XP_419265.4
Danio rerio (zebrafish)	srpk1b	NP_001074138.1
	SRPK1	XP_001116721.2
Mus musculus (house mouse)	Srpk1	NP_058075.2
Danio rerio (zebrafish)	srpk1a	NP_955944.1
Homo sapiens (human)	SRPK1	NP_003128.3
Drosophila melanogaster (fruit fly)	SRPK	NP_725458.1
Xenopus tropicalis (tropical clawed frog)	srpk1	NP_001017351.1
Canis lupus familiaris (dog)	SRPK1	XP_005627322.1
Arabidopsis thaliana (thale cress)	AT5G22840	NP_197675.1
Arabidopsis thaliana (thale cress)	AT3G44850	NP_190071.1
Arabidopsis thaliana (thale cress)	SRPK4	NP_566977.1

Gene Ontology
Bibliography

Related articles in PubMed

SRPK1?siRNA suppresses K562 cell growth and induces apoptosis via the PARP?caspase3 pathway.
Wang H, Ge W, Jiang W, Li D, Ju X
Molecular medicine reports17(1)2070-2076(2018 Jan)

Mobilization of A Splicing Factor Through A Nuclear Kinase-Kinase Complex.
Aubol BE, Keshwani MM, Fattet L, Adams JA
The Biochemical journal(2018 Jan)

Enhancer of rudimentary homologue interacts with scaffold attachment factor B at the nuclear matrix to regulate SR protein phosphorylation.
Drakouli S, Lyberopoulou A, Papathanassiou M, Mylonis I, Georgatsou E
The FEBS journal284(15)2482-2500(2017 Aug)

MicroRNA-1296 inhibits metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by targeting SRPK1-mediated PI3K/AKT pathway.
Xu Q, Liu X, Liu Z, Zhou Z, Wang Y, Tu J, Li L, Bao H, Yang L, Tu K
Molecular cancer16(1)103(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that ERH interacts directly in nucleus with C-terminal Arg-Gly-rich region of SAFB1/SAFB2 and this multimer co-localizes in insoluble nuclear fraction; binding of ERH reverses inhibition exerted by SAFB1/SAFB2 on SRPK1. (ERH = enhancer of rudimentary homolog protein; SAFB = scaffold attachment factor B; SRPK1 = splicing kinase SR protein kinase-1)
Title: Enhancer of rudimentary homologue interacts with scaffold attachment factor B at the nuclear matrix to regulate SR protein phosphorylation.

SRPK1 interference inhibits the growth and invasion of RCC cells.
Title: Knockdown of Serine-Arginine Protein Kinase 1 Inhibits the Growth and Migration in Renal Cell Carcinoma Cells.

The frameshift mutation detected in the current study would result in premature stops of amino acid synthesis in PLA2R1 and SRPK1 proteins and hence resembles a typical inactivating mutation.
Title: Inactivating frameshift mutation of putative tumor suppressor genes PLA2R1 and SRPK1 in gastric and colorectal cancers.

SRPK1 interacts with an RS-like domain in the N terminus of CLK1 to facilitate the release of phosphorylated SR proteins, which then promotes efficient splice-site recognition and subsequent spliceosome assembly.
Title: Release of SR Proteins from CLK1 by SRPK1: A Symbiotic Kinase System for Phosphorylation Control of Pre-mRNA Splicing.

Data indicate that proto-oncogene protein c-akt (Akt) phosphorylates distinct sites than SRPK1 protein within the arginine-serine (RS) domain of Lamin B Receptor (LBR).
Title: SRPK1 and Akt Protein Kinases Phosphorylate the RS Domain of Lamin B Receptor with Distinct Specificity: A Combined Biochemical and In Silico Approach.

The following SRPK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SRPK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20945	NM_003137.5 Latest version!	Homo sapiens SRSF protein kinase 1 (SRPK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
View Old Accession Versions >>
OHu20945	NM_003137.4	Homo sapiens SRSF protein kinase 1 (SRPK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SRPK1 ( NM_003137.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003128.3 Homo sapiens SRSF protein kinase 1 (SRPK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20945
Clone ID Related Accession (Same CDS sequence)	NM_003137.4 , NM_003137.5
Accession Version	NM_003137.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	SRSF protein kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB233331.1, BC038292.1, AK299591.1, Z99128.1 and BX648697.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2010 this sequence version replaced NM_003137.3. Transcript Variant: This variant (1) represents the shorter transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038292.1, SRR1660809.125029.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGA AAGTGCTTGC GCTCCAGGCC CGAAAGAAAA GGACCAAGGC CAAGAAGGAC 
AAAGCCCAAA GGAAATCTGA AACTCAGCAC CGAGGCTCTG CTCCCCACTC TGAGAGTGAT 
CTACCAGAGC AGGAAGAGGA GATTCTGGGA TCTGATGATG ATGAGCAAGA AGATCCTAAT 
GATTATTGTA AAGGAGGTTA TCATCTTGTG AAAATTGGAG ATCTATTCAA TGGGAGATAC 
CATGTGATCC GAAAGTTAGG CTGGGGACAC TTTTCAACAG TATGGTTATC ATGGGATATT 
CAGGGGAAGA AATTTGTGGC AATGAAAGTA GTTAAAAGTG CTGAACATTA CACTGAAACA 
GCACTAGATG AAATCCGGTT GCTGAAGTCA GTTCGCAATT CAGACCCTAA TGATCCAAAT 
AGAGAAATGG TTGTTCAACT ACTAGATGAC TTTAAAATAT CAGGAGTTAA TGGAACACAT 
ATCTGCATGG TATTTGAAGT TTTGGGGCAT CATCTGCTCA AGTGGATCAT CAAATCCAAT 
TATCAGGGGC TTCCACTGCC TTGTGTCAAA AAAATTATTC AGCAAGTGTT ACAGGGTCTT 
GATTATTTAC ATACCAAGTG CCGTATCATC CACACTGACA TTAAACCAGA GAACATCTTA 
TTGTCAGTGA ATGAGCAGTA CATTCGGAGG CTGGCTGCAG AAGCAACAGA ATGGCAGCGA 
TCTGGAGCTC CTCCGCCTTC CGGATCTGCA GTCAGTACTG CTCCCCAGCC TAAACCAGCT 
GACAAAATGT CAAAGAATAA GAAGAAGAAA TTGAAGAAGA AGCAGAAGCG CCAGGCAGAA 
TTACTAGAGA AGCGAATGCA GGAAATTGAG GAAATGGAGA AAGAGTCGGG CCCTGGGCAA 
AAAAGACCAA ACAAGCAAGA AGAATCAGAG AGTCCTGTTG AAAGACCCTT GAAAGAGAAC 
CCACCTAATA AAATGACCCA AGAAAAACTT GAAGAGTCAA GTACCATTGG CCAGGATCAA 
ACGCTTATGG AACGTGATAC AGAGGGTGGT GCAGCAGAAA TTAATTGCAA TGGAGTGATT 
GAAGTCATTA ATTATACTCA GAACAGTAAT AATGAAACAT TGAGACATAA AGAGGATCTA 
CATAATGCTA ATGACTGTGA TGTCCAAAAT TTGAATCAGG AATCTAGTTT CCTAAGCTCC 
CAAAATGGAG ACAGCAGCAC ATCTCAAGAA ACAGACTCTT GTACACCTAT AACATCTGAG 
GTGTCAGACA CCATGGTGTG CCAGTCTTCC TCAACTGTAG GTCAGTCATT CAGTGAACAA 
CACATTAGCC AACTTCAAGA AAGCATTCGG GCAGAGATAC CCTGTGAAGA TGAACAAGAG 
CAAGAACATA ACGGACCACT GGACAACAAA GGAAAATCCA CGGCTGGAAA TTTTCTTGTT 
AATCCCCTTG AGCCAAAAAA TGCAGAAAAG CTCAAGGTGA AGATTGCTGA CCTTGGAAAT 
GCTTGTTGGG TGCACAAACA TTTCACTGAA GATATTCAAA CAAGGCAATA TCGTTCCTTG 
GAAGTTCTAA TCGGATCTGG CTATAATACC CCTGCTGACA TTTGGAGCAC GGCATGCATG 
GCCTTTGAAC TGGCCACAGG TGACTATTTG TTTGAACCTC ATTCAGGGGA AGAGTACACT 
CGAGATGAAG ATCACATTGC ATTGATCATA GAACTTCTGG GGAAGGTGCC TCGCAAGCTC 
ATTGTGGCAG GAAAATATTC CAAGGAATTT TTCACCAAAA AAGGTGACCT GAAACATATC 
ACGAAGCTGA AACCTTGGGG CCTTTTTGAG GTTCTAGTGG AGAAGTATGA GTGGTCGCAG 
GAAGAGGCAG CTGGCTTCAC AGATTTCTTA CTGCCCATGT TGGAGCTGAT CCCTGAGAAG 
AGAGCCACTG CCGCCGAGTG TCTCCGGCAC CCTTGGCTTA ACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003128.3
CDS	125..2092
Translation	MERKVLALQARKKRTKAKKDKAQRKSETQHRGSAPHSESDLPEQEEEILGSDDDEQEDPNDYCKGGYHLVKIGDLFNGRYHVIRKLGWGHFSTVWLSWDIQGKKFVAMKVVKSAEHYTETALDEIRLLKSVRNSDPNDPNREMVVQLLDDFKISGVNGTHICMVFEVLGHHLLKWIIKSNYQGLPLPCVKKIIQQVLQGLDYLHTKCRIIHTDIKPENILLSVNEQYIRRLAAEATEWQRSGAPPPSGSAVSTAPQPKPADKMSKNKKKKLKKKQKRQAELLEKRMQEIEEMEKESGPGQKRPNKQEESESPVERPLKENPPNKMTQEKLEESSTIGQDQTLMERDTEGGAAEINCNGVIEVINYTQNSNNETLRHKEDLHNANDCDVQNLNQESSFLSSQNGDSSTSQETDSCTPITSEVSDTMVCQSSSTVGQSFSEQHISQLQESIRAEIPCEDEQEQEHNGPLDNKGKSTAGNFLVNPLEPKNAEKLKVKIADLGNACWVHKHFTEDIQTRQYRSLEVLIGSGYNTPADIWSTACMAFELATGDYLFEPHSGEEYTRDEDHIALIIELLGKVPRKLIVAGKYSKEFFTKKGDLKHITKLKPWGLFEVLVEKYEWSQEEAAGFTDFLLPMLELIPEKRATAAECLRHPWLNS

Target ORF information:

RefSeq Version	NM_003137.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SRSF protein kinase 1 (SRPK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003137.4

ORF Insert Sequence:

ATGGAGCGGA AAGTGCTTGC GCTCCAGGCC CGAAAGAAAA GGACCAAGGC CAAGAAGGAC 
AAAGCCCAAA GGAAATCTGA AACTCAGCAC CGAGGCTCTG CTCCCCACTC TGAGAGTGAT 
CTACCAGAGC AGGAAGAGGA GATTCTGGGA TCTGATGATG ATGAGCAAGA AGATCCTAAT 
GATTATTGTA AAGGAGGTTA TCATCTTGTG AAAATTGGAG ATCTATTCAA TGGGAGATAC 
CATGTGATCC GAAAGTTAGG CTGGGGACAC TTTTCAACAG TATGGTTATC ATGGGATATT 
CAGGGGAAGA AATTTGTGGC AATGAAAGTA GTTAAAAGTG CTGAACATTA CACTGAAACA 
GCACTAGATG AAATCCGGTT GCTGAAGTCA GTTCGCAATT CAGACCCTAA TGATCCAAAT 
AGAGAAATGG TTGTTCAACT ACTAGATGAC TTTAAAATAT CAGGAGTTAA TGGAACACAT 
ATCTGCATGG TATTTGAAGT TTTGGGGCAT CATCTGCTCA AGTGGATCAT CAAATCCAAT 
TATCAGGGGC TTCCACTGCC TTGTGTCAAA AAAATTATTC AGCAAGTGTT ACAGGGTCTT 
GATTATTTAC ATACCAAGTG CCGTATCATC CACACTGACA TTAAACCAGA GAACATCTTA 
TTGTCAGTGA ATGAGCAGTA CATTCGGAGG CTGGCTGCAG AAGCAACAGA ATGGCAGCGA 
TCTGGAGCTC CTCCGCCTTC CGGATCTGCA GTCAGTACTG CTCCCCAGCC TAAACCAGCT 
GACAAAATGT CAAAGAATAA GAAGAAGAAA TTGAAGAAGA AGCAGAAGCG CCAGGCAGAA 
TTACTAGAGA AGCGAATGCA GGAAATTGAG GAAATGGAGA AAGAGTCGGG CCCTGGGCAA 
AAAAGACCAA ACAAGCAAGA AGAATCAGAG AGTCCTGTTG AAAGACCCTT GAAAGAGAAC 
CCACCTAATA AAATGACCCA AGAAAAACTT GAAGAGTCAA GTACCATTGG CCAGGATCAA 
ACGCTTATGG AACGTGATAC AGAGGGTGGT GCAGCAGAAA TTAATTGCAA TGGAGTGATT 
GAAGTCATTA ATTATACTCA GAACAGTAAT AATGAAACAT TGAGACATAA AGAGGATCTA 
CATAATGCTA ATGACTGTGA TGTCCAAAAT TTGAATCAGG AATCTAGTTT CCTAAGCTCC 
CAAAATGGAG ACAGCAGCAC ATCTCAAGAA ACAGACTCTT GTACACCTAT AACATCTGAG 
GTGTCAGACA CCATGGTGTG CCAGTCTTCC TCAACTGTAG GTCAGTCATT CAGTGAACAA 
CACATTAGCC AACTTCAAGA AAGCATTCGG GCAGAGATAC CCTGTGAAGA TGAACAAGAG 
CAAGAACATA ACGGACCACT GGACAACAAA GGAAAATCCA CGGCTGGAAA TTTTCTTGTT 
AATCCCCTTG AGCCAAAAAA TGCAGAAAAG CTCAAGGTGA AGATTGCTGA CCTTGGAAAT 
GCTTGTTGGG TGCACAAACA TTTCACTGAA GATATTCAAA CAAGGCAATA TCGTTCCTTG 
GAAGTTCTAA TCGGATCTGG CTATAATACC CCTGCTGACA TTTGGAGCAC GGCATGCATG 
GCCTTTGAAC TGGCCACAGG TGACTATTTG TTTGAACCTC ATTCAGGGGA AGAGTACACT 
CGAGATGAAG ATCACATTGC ATTGATCATA GAACTTCTGG GGAAGGTGCC TCGCAAGCTC 
ATTGTGGCAG GAAAATATTC CAAGGAATTT TTCACCAAAA AAGGTGACCT GAAACATATC 
ACGAAGCTGA AACCTTGGGG CCTTTTTGAG GTTCTAGTGG AGAAGTATGA GTGGTCGCAG 
GAAGAGGCAG CTGGCTTCAC AGATTTCTTA CTGCCCATGT TGGAGCTGAT CCCTGAGAAG 
AGAGCCACTG CCGCCGAGTG TCTCCGGCAC CCTTGGCTTA ACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRPK1 ( NM_003137.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003128.3 Homo sapiens SRSF protein kinase 1 (SRPK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20945
Clone ID Related Accession (Same CDS sequence)	NM_003137.4 , NM_003137.5
Accession Version	NM_003137.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	SRSF protein kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC038292.1, AK299591.1, Z99128.1 and BX648697.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_003137.4. Transcript Variant: This variant (1) represents the shorter transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.85279.1, SRR1803613.123013.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373825.7/ ENSP00000362931.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCGGA AAGTGCTTGC GCTCCAGGCC CGAAAGAAAA GGACCAAGGC CAAGAAGGAC 
AAAGCCCAAA GGAAATCTGA AACTCAGCAC CGAGGCTCTG CTCCCCACTC TGAGAGTGAT 
CTACCAGAGC AGGAAGAGGA GATTCTGGGA TCTGATGATG ATGAGCAAGA AGATCCTAAT 
GATTATTGTA AAGGAGGTTA TCATCTTGTG AAAATTGGAG ATCTATTCAA TGGGAGATAC 
CATGTGATCC GAAAGTTAGG CTGGGGACAC TTTTCAACAG TATGGTTATC ATGGGATATT 
CAGGGGAAGA AATTTGTGGC AATGAAAGTA GTTAAAAGTG CTGAACATTA CACTGAAACA 
GCACTAGATG AAATCCGGTT GCTGAAGTCA GTTCGCAATT CAGACCCTAA TGATCCAAAT 
AGAGAAATGG TTGTTCAACT ACTAGATGAC TTTAAAATAT CAGGAGTTAA TGGAACACAT 
ATCTGCATGG TATTTGAAGT TTTGGGGCAT CATCTGCTCA AGTGGATCAT CAAATCCAAT 
TATCAGGGGC TTCCACTGCC TTGTGTCAAA AAAATTATTC AGCAAGTGTT ACAGGGTCTT 
GATTATTTAC ATACCAAGTG CCGTATCATC CACACTGACA TTAAACCAGA GAACATCTTA 
TTGTCAGTGA ATGAGCAGTA CATTCGGAGG CTGGCTGCAG AAGCAACAGA ATGGCAGCGA 
TCTGGAGCTC CTCCGCCTTC CGGATCTGCA GTCAGTACTG CTCCCCAGCC TAAACCAGCT 
GACAAAATGT CAAAGAATAA GAAGAAGAAA TTGAAGAAGA AGCAGAAGCG CCAGGCAGAA 
TTACTAGAGA AGCGAATGCA GGAAATTGAG GAAATGGAGA AAGAGTCGGG CCCTGGGCAA 
AAAAGACCAA ACAAGCAAGA AGAATCAGAG AGTCCTGTTG AAAGACCCTT GAAAGAGAAC 
CCACCTAATA AAATGACCCA AGAAAAACTT GAAGAGTCAA GTACCATTGG CCAGGATCAA 
ACGCTTATGG AACGTGATAC AGAGGGTGGT GCAGCAGAAA TTAATTGCAA TGGAGTGATT 
GAAGTCATTA ATTATACTCA GAACAGTAAT AATGAAACAT TGAGACATAA AGAGGATCTA 
CATAATGCTA ATGACTGTGA TGTCCAAAAT TTGAATCAGG AATCTAGTTT CCTAAGCTCC 
CAAAATGGAG ACAGCAGCAC ATCTCAAGAA ACAGACTCTT GTACACCTAT AACATCTGAG 
GTGTCAGACA CCATGGTGTG CCAGTCTTCC TCAACTGTAG GTCAGTCATT CAGTGAACAA 
CACATTAGCC AACTTCAAGA AAGCATTCGG GCAGAGATAC CCTGTGAAGA TGAACAAGAG 
CAAGAACATA ACGGACCACT GGACAACAAA GGAAAATCCA CGGCTGGAAA TTTTCTTGTT 
AATCCCCTTG AGCCAAAAAA TGCAGAAAAG CTCAAGGTGA AGATTGCTGA CCTTGGAAAT 
GCTTGTTGGG TGCACAAACA TTTCACTGAA GATATTCAAA CAAGGCAATA TCGTTCCTTG 
GAAGTTCTAA TCGGATCTGG CTATAATACC CCTGCTGACA TTTGGAGCAC GGCATGCATG 
GCCTTTGAAC TGGCCACAGG TGACTATTTG TTTGAACCTC ATTCAGGGGA AGAGTACACT 
CGAGATGAAG ATCACATTGC ATTGATCATA GAACTTCTGG GGAAGGTGCC TCGCAAGCTC 
ATTGTGGCAG GAAAATATTC CAAGGAATTT TTCACCAAAA AAGGTGACCT GAAACATATC 
ACGAAGCTGA AACCTTGGGG CCTTTTTGAG GTTCTAGTGG AGAAGTATGA GTGGTCGCAG 
GAAGAGGCAG CTGGCTTCAC AGATTTCTTA CTGCCCATGT TGGAGCTGAT CCCTGAGAAG 
AGAGCCACTG CCGCCGAGTG TCTCCGGCAC CCTTGGCTTA ACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003128.3
CDS	43..2010
Translation	MERKVLALQARKKRTKAKKDKAQRKSETQHRGSAPHSESDLPEQEEEILGSDDDEQEDPNDYCKGGYHLVKIGDLFNGRYHVIRKLGWGHFSTVWLSWDIQGKKFVAMKVVKSAEHYTETALDEIRLLKSVRNSDPNDPNREMVVQLLDDFKISGVNGTHICMVFEVLGHHLLKWIIKSNYQGLPLPCVKKIIQQVLQGLDYLHTKCRIIHTDIKPENILLSVNEQYIRRLAAEATEWQRSGAPPPSGSAVSTAPQPKPADKMSKNKKKKLKKKQKRQAELLEKRMQEIEEMEKESGPGQKRPNKQEESESPVERPLKENPPNKMTQEKLEESSTIGQDQTLMERDTEGGAAEINCNGVIEVINYTQNSNNETLRHKEDLHNANDCDVQNLNQESSFLSSQNGDSSTSQETDSCTPITSEVSDTMVCQSSSTVGQSFSEQHISQLQESIRAEIPCEDEQEQEHNGPLDNKGKSTAGNFLVNPLEPKNAEKLKVKIADLGNACWVHKHFTEDIQTRQYRSLEVLIGSGYNTPADIWSTACMAFELATGDYLFEPHSGEEYTRDEDHIALIIELLGKVPRKLIVAGKYSKEFFTKKGDLKHITKLKPWGLFEVLVEKYEWSQEEAAGFTDFLLPMLELIPEKRATAAECLRHPWLNS

Target ORF information:

RefSeq Version	NM_003137.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SRSF protein kinase 1 (SRPK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003137.5

ORF Insert Sequence:

ATGGAGCGGA AAGTGCTTGC GCTCCAGGCC CGAAAGAAAA GGACCAAGGC CAAGAAGGAC 
AAAGCCCAAA GGAAATCTGA AACTCAGCAC CGAGGCTCTG CTCCCCACTC TGAGAGTGAT 
CTACCAGAGC AGGAAGAGGA GATTCTGGGA TCTGATGATG ATGAGCAAGA AGATCCTAAT 
GATTATTGTA AAGGAGGTTA TCATCTTGTG AAAATTGGAG ATCTATTCAA TGGGAGATAC 
CATGTGATCC GAAAGTTAGG CTGGGGACAC TTTTCAACAG TATGGTTATC ATGGGATATT 
CAGGGGAAGA AATTTGTGGC AATGAAAGTA GTTAAAAGTG CTGAACATTA CACTGAAACA 
GCACTAGATG AAATCCGGTT GCTGAAGTCA GTTCGCAATT CAGACCCTAA TGATCCAAAT 
AGAGAAATGG TTGTTCAACT ACTAGATGAC TTTAAAATAT CAGGAGTTAA TGGAACACAT 
ATCTGCATGG TATTTGAAGT TTTGGGGCAT CATCTGCTCA AGTGGATCAT CAAATCCAAT 
TATCAGGGGC TTCCACTGCC TTGTGTCAAA AAAATTATTC AGCAAGTGTT ACAGGGTCTT 
GATTATTTAC ATACCAAGTG CCGTATCATC CACACTGACA TTAAACCAGA GAACATCTTA 
TTGTCAGTGA ATGAGCAGTA CATTCGGAGG CTGGCTGCAG AAGCAACAGA ATGGCAGCGA 
TCTGGAGCTC CTCCGCCTTC CGGATCTGCA GTCAGTACTG CTCCCCAGCC TAAACCAGCT 
GACAAAATGT CAAAGAATAA GAAGAAGAAA TTGAAGAAGA AGCAGAAGCG CCAGGCAGAA 
TTACTAGAGA AGCGAATGCA GGAAATTGAG GAAATGGAGA AAGAGTCGGG CCCTGGGCAA 
AAAAGACCAA ACAAGCAAGA AGAATCAGAG AGTCCTGTTG AAAGACCCTT GAAAGAGAAC 
CCACCTAATA AAATGACCCA AGAAAAACTT GAAGAGTCAA GTACCATTGG CCAGGATCAA 
ACGCTTATGG AACGTGATAC AGAGGGTGGT GCAGCAGAAA TTAATTGCAA TGGAGTGATT 
GAAGTCATTA ATTATACTCA GAACAGTAAT AATGAAACAT TGAGACATAA AGAGGATCTA 
CATAATGCTA ATGACTGTGA TGTCCAAAAT TTGAATCAGG AATCTAGTTT CCTAAGCTCC 
CAAAATGGAG ACAGCAGCAC ATCTCAAGAA ACAGACTCTT GTACACCTAT AACATCTGAG 
GTGTCAGACA CCATGGTGTG CCAGTCTTCC TCAACTGTAG GTCAGTCATT CAGTGAACAA 
CACATTAGCC AACTTCAAGA AAGCATTCGG GCAGAGATAC CCTGTGAAGA TGAACAAGAG 
CAAGAACATA ACGGACCACT GGACAACAAA GGAAAATCCA CGGCTGGAAA TTTTCTTGTT 
AATCCCCTTG AGCCAAAAAA TGCAGAAAAG CTCAAGGTGA AGATTGCTGA CCTTGGAAAT 
GCTTGTTGGG TGCACAAACA TTTCACTGAA GATATTCAAA CAAGGCAATA TCGTTCCTTG 
GAAGTTCTAA TCGGATCTGG CTATAATACC CCTGCTGACA TTTGGAGCAC GGCATGCATG 
GCCTTTGAAC TGGCCACAGG TGACTATTTG TTTGAACCTC ATTCAGGGGA AGAGTACACT 
CGAGATGAAG ATCACATTGC ATTGATCATA GAACTTCTGG GGAAGGTGCC TCGCAAGCTC 
ATTGTGGCAG GAAAATATTC CAAGGAATTT TTCACCAAAA AAGGTGACCT GAAACATATC 
ACGAAGCTGA AACCTTGGGG CCTTTTTGAG GTTCTAGTGG AGAAGTATGA GTGGTCGCAG 
GAAGAGGCAG CTGGCTTCAC AGATTTCTTA CTGCCCATGT TGGAGCTGAT CCCTGAGAAG 
AGAGCCACTG CCGCCGAGTG TCTCCGGCAC CCTTGGCTTA ACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SRPK1?siRNA suppresses K562 cell growth and induces apoptosis via the PARP?caspase3 pathway.
Molecular medicine reports17(1)2070-2076(2018 Jan)
Wang H,Ge W,Jiang W,Li D,Ju X

Mobilization of A Splicing Factor Through A Nuclear Kinase-Kinase Complex.
The Biochemical journal(2018 Jan)
Aubol BE,Keshwani MM,Fattet L,Adams JA

Enhancer of rudimentary homologue interacts with scaffold attachment factor B at the nuclear matrix to regulate SR protein phosphorylation.
The FEBS journal284(15)2482-2500(2017 Aug)
Drakouli S,Lyberopoulou A,Papathanassiou M,Mylonis I,Georgatsou E

MicroRNA-1296 inhibits metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by targeting SRPK1-mediated PI3K/AKT pathway.
Molecular cancer16(1)103(2017 Jun)
Xu Q,Liu X,Liu Z,Zhou Z,Wang Y,Tu J,Li L,Bao H,Yang L,Tu K

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SRPK1 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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