ORF » Species Summary » Homo sapiens » BRAF cDNA ORF clone

BRAF cDNA ORF clone, Homo sapiens(human)

The following BRAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu28570 NM_004333.6
Latest version!		 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$279.50-$391.30
$559.00
OHu109307 NM_001374244.1
Latest version!		 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu97466 NM_001374258.1
Latest version!		 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu41737 NM_001354609.2
Latest version!		 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu41737 XM_005250045.2
Latest version!		 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu97466 XM_017012558.1
Latest version!		 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu97467 XM_017012559.1
Latest version!		 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
View Old Accession Versions >>
OHu28570 NM_004333.4 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$279.50-$391.30
$559.00
OHu28570 NM_004333.5 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$279.50-$391.30
$559.00
OHu41737 NM_001354609.1 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    BRAF ( NM_004333.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004324.2 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.
    Price & Availability↑
    CloneID OHu28570
    Clone ID Related Accession (Same CDS sequence) NM_004333.4 , NM_004333.6 , NM_004333.5
    Accession Version NM_004333.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-06
    Organism Homo sapiens(Human)
    Product serine/threonine-protein kinase B-raf
    Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB454480.1, BC101757.1, M95712.2, AW971583.1 and AA252358.1. This sequence is a reference standard in the RefSeqGene project. On May 7, 2008 this sequence version replaced NM_004333.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M95712.2, BC101757.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGTGCGTTTC CTGTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004324.2
    CDS62..2362
    Translation

    Target ORF information:

    RefSeq Version NM_004333.4
    Organism Homo sapiens(Human)
    Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_004333.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGTGCGTTTC CTGTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BRAF ( NM_004333.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_004324.2 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu28570
    Clone ID Related Accession (Same CDS sequence) NM_004333.4 , NM_004333.6 , NM_004333.5
    Accession Version NM_004333.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase B-raf isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006006.2, DB454480.1, BC101757.1, M95712.2, AW971583.1 and AC006344.2. On Jun 2, 2019 this sequence version replaced NM_004333.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M95712.2, BC101757.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGTGCGTTTC CTGTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004324.2
    CDS227..2527
    Translation

    Target ORF information:

    RefSeq Version NM_004333.6
    Organism Homo sapiens(human)
    Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_004333.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGTGCGTTTC CTGTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BRAF ( NM_004333.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004324.2 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu28570
    Clone ID Related Accession (Same CDS sequence) NM_004333.4 , NM_004333.6 , NM_004333.5
    Accession Version NM_004333.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase B-raf isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB454480.1, BC101757.1, M95712.2, AW971583.1 and AC006344.2. On Aug 16, 2017 this sequence version replaced NM_004333.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M95712.2, BC101757.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGTGCGTTTC CTGTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004324.2
    CDS226..2526
    Translation

    Target ORF information:

    RefSeq Version NM_004333.5
    Organism Homo sapiens(human)
    Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_004333.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGTGCGTTTC CTGTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BRAF ( NM_001374244.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361173.1 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 4, mRNA.
    Price & Availability↑
    CloneID OHu109307
    Clone ID Related Accession (Same CDS sequence) NM_001374244.1
    Accession Version NM_001374244.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase B-raf isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006006.2, AC006347.2 and AC006344.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on manual assertion, conservation ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC 
    TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA 
    ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGTGCGTTTC CTGTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361173.1
    CDS227..2647
    Translation

    Target ORF information:

    RefSeq Version NM_001374244.1
    Organism Homo sapiens(human)
    Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001374244.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC 
    TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA 
    ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGTGCGTTTC CTGTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BRAF ( NM_001374258.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361187.1 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 5, mRNA.
    Price & Availability↑
    CloneID OHu97466
    Clone ID Related Accession (Same CDS sequence) XM_017012558.1 , NM_001374258.1
    Accession Version NM_001374258.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2424bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase B-raf isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006006.2, AC006347.2 and AC006344.2. On Sep 17, 2019 this sequence version replaced XM_017012558.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC 
    TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA 
    ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361187.1
    CDS227..2650
    Translation

    Target ORF information:

    RefSeq Version NM_001374258.1
    Organism Homo sapiens(human)
    Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001374258.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC 
    TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA 
    ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BRAF ( NM_001354609.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341538.1 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu41737
    Clone ID Related Accession (Same CDS sequence) XM_005250045.2 , NM_001354609.2 , NM_001354609.1
    Accession Version NM_001354609.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase B-raf isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006006.2, AC006347.2, AC006344.2 and AA975673.1. On Jun 2, 2019 this sequence version replaced NM_001354609.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.151600.1, SRR1803616.97221.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341538.1
    CDS227..2530
    Translation

    Target ORF information:

    RefSeq Version NM_001354609.2
    Organism Homo sapiens(human)
    Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001354609.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BRAF ( NM_001354609.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341538.1 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu41737
    Clone ID Related Accession (Same CDS sequence) XM_005250045.2 , NM_001354609.2 , NM_001354609.1
    Accession Version NM_001354609.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase B-raf isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006006.2, AC006347.2, AC006344.2 and AA975673.1. On Aug 29, 2017 this sequence version replaced XM_005250045.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.13769.1, SRR1660809.204619.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341538.1
    CDS226..2529
    Translation

    Target ORF information:

    RefSeq Version NM_001354609.1
    Organism Homo sapiens(human)
    Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001354609.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BRAF ( XM_005250045.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005250102.1 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OHu41737
    Clone ID Related Accession (Same CDS sequence) XM_005250045.2 , NM_001354609.2 , NM_001354609.1
    Accession Version XM_005250045.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product serine/threonine-protein kinase B-raf isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005250045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250102.1
    CDS9..2312
    Translation

    Target ORF information:

    RefSeq Version XM_005250045.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005250045.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BRAF ( XM_017012558.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868047.1 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu97466
    Clone ID Related Accession (Same CDS sequence) XM_017012558.1 , NM_001374258.1
    Accession Version XM_017012558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2424bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase B-raf isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC 
    TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA 
    ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868047.1
    CDS9..2432
    Translation

    Target ORF information:

    RefSeq Version XM_017012558.1
    Organism Homo sapiens(human)
    Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017012558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC 
    TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA 
    ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BRAF ( XM_017012559.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868048.1 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OHu97467
    Clone ID Related Accession (Same CDS sequence) XM_017012559.1
    Accession Version XM_017012559.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2397bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase B-raf isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC 
    TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA 
    ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAGAG AATTTGCAGC CTTCAAGTAG CCACCATCAT 
    GGCAGCATCT GCTCTTATTT CTTAAGTCTT GTGTTCGTAC AATTTGTTAA CATCAAAACA 
    CAGTTCTGTT CCTCAAATCT TTTTTTAAAG ATACAAAATT TCCAATGCAT AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868048.1
    CDS9..2405
    Translation

    Target ORF information:

    RefSeq Version XM_017012559.1
    Organism Homo sapiens(human)
    Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017012559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
    GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 
    CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 
    ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 
    GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 
    GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 
    ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 
    CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 
    CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 
    CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 
    CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 
    GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 
    ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 
    TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 
    GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 
    CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 
    CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 
    CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 
    GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 
    GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC 
    TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA 
    ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT 
    ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 
    GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 
    CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
    CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
    GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 
    GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 
    ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 
    CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 
    GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
    AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 
    AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 
    GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 
    GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 
    AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 
    GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
    AGAGATGAGA GACCACTCTT TCCCCAAGAG AATTTGCAGC CTTCAAGTAG CCACCATCAT 
    GGCAGCATCT GCTCTTATTT CTTAAGTCTT GTGTTCGTAC AATTTGTTAA CATCAAAACA 
    CAGTTCTGTT CCTCAAATCT TTTTTTAAAG ATACAAAATT TCCAATGCAT AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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