AKR1D1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AKR1D1
Entrez Gene ID	6718
Full Name	aldo-keto reductase family 1 member D1
Synonyms	3o5bred,CBAS2,SRD5B1
General protein information	Preferred Names aldo-keto reductase family 1 member D1 Names 3-oxo-5-beta-steroid 4-dehydrogenase delta(4)-3-ketosteroid 5-beta-reductase delta(4)-3-oxosteroid 5-beta-reductase steroid-5-beta-reductase, beta polypeptide 1 (3-oxo-5 beta-steroid delta 4-dehydrogenase beta 1)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q33
Summary	The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010].
Disorder MIM:	604741
Disorder Html:	Bile acid synthesis defect, congenital, 2, 235555 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001190906.1	NP_001177835.1	3-oxo-5-beta-steroid 4-dehydrogenase isoform 2
NM_001190907.1	NP_001177836.1	3-oxo-5-beta-steroid 4-dehydrogenase isoform 3
NM_001190906.2	NP_001177835.1	aldo-keto reductase family 1 member D1 isoform 2
NM_001190907.2	NP_001177836.1	aldo-keto reductase family 1 member D1 isoform 3
NM_005989.4	NP_005980.1	aldo-keto reductase family 1 member D1 isoform 1
NM_005989.3	NP_005980.1	3-oxo-5-beta-steroid 4-dehydrogenase isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	AKR1D1	NP_005980.1
	AKR1D1	XP_001107157.1
Canis lupus familiaris (dog)	AKR1D1	XP_005629673.1
Bos taurus (cattle)	AKR1D1	NP_001179287.1
Mus musculus (house mouse)	Akr1d1	NP_663339.1
Rattus norvegicus (Norway rat)	Akr1d1	NP_620239.1
Xenopus tropicalis (tropical clawed frog)	akr1d1	NP_001025609.1
Pan troglodytes (chimpanzee)	AKR1D1	XP_001148679.1
Gallus gallus (chicken)	AKR1D1	NP_001264322.1

Gene Ontology
Bibliography

Related articles in PubMed

Dysregulation of ?
Valanejad L, Ghareeb M, Shiffka S, Nadolny C, Chen Y, Guo L, Verma R, You S, Akhlaghi F, Deng R
Molecular and cellular endocrinology(2017 Oct)

[Clinical feature and genetic analysis of a family affected by congenital bile acid synthesis defect type 2: identification of 2 novel mutations in AKR1D1 gene].
Cheng Y, Guo L, Deng M, Song YZ
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics19(7)734-740(2017 Jul)

In-Depth Dissection of the P133R Mutation in Steroid 5?-Reductase (AKR1D1): A Molecular Basis of Bile Acid Deficiency.
Chen M, Jin Y, Penning TM
Biochemistry54(41)6343-51(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

infant proved to be a compound heterozygote of the AKR1D1 variants c.579+2delT and c.853C>T(p.Q285X), two novel mutations originated from his mother and father, respectively
Title: [Clinical feature and genetic analysis of a family affected by congenital bile acid synthesis defect type 2: identification of 2 novel mutations in AKR1D1 gene].

Impaired NADPH binding and hydride transfer is the molecular basis for bile acid deficiency in patients with the P133R mutation in AKR1D1.
Title: In-Depth Dissection of the P133R Mutation in Steroid 5?-Reductase (AKR1D1): A Molecular Basis of Bile Acid Deficiency.

When different steroid substrates were used in single turnover experiments with AKR1D1.
Title: The rate-determining steps of aldo-keto reductases (AKRs), a study on human steroid 5?-reductase (AKR1D1).

Despite having high kchem values with steroid hormones, the kinetic control of AKR1D1 is consistent with the enzyme catalysing the slowest step in the catabolic sequence of steroid hormone transformation in the liver.
Title: Rate of steroid double-bond reduction catalysed by the human steroid 5?-reductase (AKR1D1) is sensitive to steroid structure: implications for steroid metabolism and bile acid synthesis.

AKR1D1 generates all 5beta-dihydrosteroids in the C19-C27 steroid series.
Title: 5?-Reduced steroids and human ?(4)-3-ketosteroid 5?-reductase (AKR1D1).

The following AKR1D1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKR1D1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22326	NM_001190907.2 Latest version!	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu22861	NM_005989.4 Latest version!	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu22961	NM_001190906.2 Latest version!	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
View Old Accession Versions >>
OHu22961	NM_001190906.1	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu22326	NM_001190907.1	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu22861	NM_005989.3	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AKR1D1 ( NM_001190907.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177836.1 Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22326
Clone ID Related Accession (Same CDS sequence)	NM_001190907.1 , NM_001190907.2
Accession Version	NM_001190907.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	3-oxo-5-beta-steroid 4-dehydrogenase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298421.1 and AC083867.7. Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298421.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGCGA TGGAAGCTTG CAAAGACGCT 
GGCTTGGTGA AATCCCTGGG AGTGTCCAAT TTTAACCGCA GGCAGCTGGA GCTCATCCTG 
AACAAGCCAG GACTCAAACA CAAGCCAGTC AGCAACCAGG TTGAGTGCCA TCCGTATTTC 
ACCCAGCCAA AACTCTTGAA ATTTTGCCAA CAACATGACA TTGTCATTAC TGCATATAGC 
CCTTTGGGGA CCAGTAGGAA TCCAATCTGG GTGAATGTTT CTTCTCCACC TTTGTTAAAG 
GATGCACTTC TAAACTCATT GGGGAAAAGG TACAATAAGA CAGCAGCTCA AATTGTTTTG 
CGTTTCAACA TCCAGCGAGG GGTGGTTGTC ATTCCTAAAA GCTTTAATCT TGAAAGGATC 
AAAGAAAATT TTCAGGTGGC GCGATCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177836.1
CDS	88..960
Translation	MDLSAASHRIPLSDGNSIPIIGLGTYSEPKSTPKGACATSVKVAIDTGYRHIDGAYIYQNEHEVGEAIREKIAEGKVRREDIFYCGKLWATNHVPEMVRPTLERTLRVLQLDYVDLYIIEVPMAFKPGDEIYPRDENGKWLYHKSNLCATWEAMEACKDAGLVKSLGVSNFNRRQLELILNKPGLKHKPVSNQVECHPYFTQPKLLKFCQQHDIVITAYSPLGTSRNPIWVNVSSPPLLKDALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQVARSS

Target ORF information:

RefSeq Version	NM_001190907.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190907.1

ORF Insert Sequence:

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGCGA TGGAAGCTTG CAAAGACGCT 
GGCTTGGTGA AATCCCTGGG AGTGTCCAAT TTTAACCGCA GGCAGCTGGA GCTCATCCTG 
AACAAGCCAG GACTCAAACA CAAGCCAGTC AGCAACCAGG TTGAGTGCCA TCCGTATTTC 
ACCCAGCCAA AACTCTTGAA ATTTTGCCAA CAACATGACA TTGTCATTAC TGCATATAGC 
CCTTTGGGGA CCAGTAGGAA TCCAATCTGG GTGAATGTTT CTTCTCCACC TTTGTTAAAG 
GATGCACTTC TAAACTCATT GGGGAAAAGG TACAATAAGA CAGCAGCTCA AATTGTTTTG 
CGTTTCAACA TCCAGCGAGG GGTGGTTGTC ATTCCTAAAA GCTTTAATCT TGAAAGGATC 
AAAGAAAATT TTCAGGTGGC GCGATCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKR1D1 ( NM_001190907.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177836.1 Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22326
Clone ID Related Accession (Same CDS sequence)	NM_001190907.1 , NM_001190907.2
Accession Version	NM_001190907.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	aldo-keto reductase family 1 member D1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298421.1 and AC083867.7. On Jun 1, 2019 this sequence version replaced NM_001190907.1. Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298421.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGCGA TGGAAGCTTG CAAAGACGCT 
GGCTTGGTGA AATCCCTGGG AGTGTCCAAT TTTAACCGCA GGCAGCTGGA GCTCATCCTG 
AACAAGCCAG GACTCAAACA CAAGCCAGTC AGCAACCAGG TTGAGTGCCA TCCGTATTTC 
ACCCAGCCAA AACTCTTGAA ATTTTGCCAA CAACATGACA TTGTCATTAC TGCATATAGC 
CCTTTGGGGA CCAGTAGGAA TCCAATCTGG GTGAATGTTT CTTCTCCACC TTTGTTAAAG 
GATGCACTTC TAAACTCATT GGGGAAAAGG TACAATAAGA CAGCAGCTCA AATTGTTTTG 
CGTTTCAACA TCCAGCGAGG GGTGGTTGTC ATTCCTAAAA GCTTTAATCT TGAAAGGATC 
AAAGAAAATT TTCAGGTGGC GCGATCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177836.1
CDS	61..933
Translation	MDLSAASHRIPLSDGNSIPIIGLGTYSEPKSTPKGACATSVKVAIDTGYRHIDGAYIYQNEHEVGEAIREKIAEGKVRREDIFYCGKLWATNHVPEMVRPTLERTLRVLQLDYVDLYIIEVPMAFKPGDEIYPRDENGKWLYHKSNLCATWEAMEACKDAGLVKSLGVSNFNRRQLELILNKPGLKHKPVSNQVECHPYFTQPKLLKFCQQHDIVITAYSPLGTSRNPIWVNVSSPPLLKDALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQVARSS

Target ORF information:

RefSeq Version	NM_001190907.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190907.2

ORF Insert Sequence:

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGCGA TGGAAGCTTG CAAAGACGCT 
GGCTTGGTGA AATCCCTGGG AGTGTCCAAT TTTAACCGCA GGCAGCTGGA GCTCATCCTG 
AACAAGCCAG GACTCAAACA CAAGCCAGTC AGCAACCAGG TTGAGTGCCA TCCGTATTTC 
ACCCAGCCAA AACTCTTGAA ATTTTGCCAA CAACATGACA TTGTCATTAC TGCATATAGC 
CCTTTGGGGA CCAGTAGGAA TCCAATCTGG GTGAATGTTT CTTCTCCACC TTTGTTAAAG 
GATGCACTTC TAAACTCATT GGGGAAAAGG TACAATAAGA CAGCAGCTCA AATTGTTTTG 
CGTTTCAACA TCCAGCGAGG GGTGGTTGTC ATTCCTAAAA GCTTTAATCT TGAAAGGATC 
AAAGAAAATT TTCAGGTGGC GCGATCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKR1D1 ( NM_005989.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005980.1 Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22861
Clone ID Related Accession (Same CDS sequence)	NM_005989.4 , NM_005989.3
Accession Version	NM_005989.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	aldo-keto reductase family 1 member D1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z28339.1 and AC083867.7. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005989.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z28339.1, BC130625.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000242375.8/ ENSP00000242375.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGCGA TGGAAGCTTG CAAAGACGCT 
GGCTTGGTGA AATCCCTGGG AGTGTCCAAT TTTAACCGCA GGCAGCTGGA GCTCATCCTG 
AACAAGCCAG GACTCAAACA CAAGCCAGTC AGCAACCAGG TTGAGTGCCA TCCGTATTTC 
ACCCAGCCAA AACTCTTGAA ATTTTGCCAA CAACATGACA TTGTCATTAC TGCATATAGC 
CCTTTGGGGA CCAGTAGGAA TCCAATCTGG GTGAATGTTT CTTCTCCACC TTTGTTAAAG 
GATGCACTTC TAAACTCATT GGGGAAAAGG TACAATAAGA CAGCAGCTCA AATTGTTTTG 
CGTTTCAACA TCCAGCGAGG GGTGGTTGTC ATTCCTAAAA GCTTTAATCT TGAAAGGATC 
AAAGAAAATT TTCAGATCTT TGACTTTTCT CTCACTGAAG AAGAAATGAA GGACATTGAA 
GCCTTGAATA AAAATGTCCG CTTTGTAGAA TTGCTCATGT GGCGCGATCA TCCTGAATAC 
CCATTTCATG ATGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005980.1
CDS	61..1041
Translation	MDLSAASHRIPLSDGNSIPIIGLGTYSEPKSTPKGACATSVKVAIDTGYRHIDGAYIYQNEHEVGEAIREKIAEGKVRREDIFYCGKLWATNHVPEMVRPTLERTLRVLQLDYVDLYIIEVPMAFKPGDEIYPRDENGKWLYHKSNLCATWEAMEACKDAGLVKSLGVSNFNRRQLELILNKPGLKHKPVSNQVECHPYFTQPKLLKFCQQHDIVITAYSPLGTSRNPIWVNVSSPPLLKDALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQIFDFSLTEEEMKDIEALNKNVRFVELLMWRDHPEYPFHDEY

Target ORF information:

RefSeq Version	NM_005989.4
Organism	Homo sapiens(human)
Definition	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005989.4

ORF Insert Sequence:

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGCGA TGGAAGCTTG CAAAGACGCT 
GGCTTGGTGA AATCCCTGGG AGTGTCCAAT TTTAACCGCA GGCAGCTGGA GCTCATCCTG 
AACAAGCCAG GACTCAAACA CAAGCCAGTC AGCAACCAGG TTGAGTGCCA TCCGTATTTC 
ACCCAGCCAA AACTCTTGAA ATTTTGCCAA CAACATGACA TTGTCATTAC TGCATATAGC 
CCTTTGGGGA CCAGTAGGAA TCCAATCTGG GTGAATGTTT CTTCTCCACC TTTGTTAAAG 
GATGCACTTC TAAACTCATT GGGGAAAAGG TACAATAAGA CAGCAGCTCA AATTGTTTTG 
CGTTTCAACA TCCAGCGAGG GGTGGTTGTC ATTCCTAAAA GCTTTAATCT TGAAAGGATC 
AAAGAAAATT TTCAGATCTT TGACTTTTCT CTCACTGAAG AAGAAATGAA GGACATTGAA 
GCCTTGAATA AAAATGTCCG CTTTGTAGAA TTGCTCATGT GGCGCGATCA TCCTGAATAC 
CCATTTCATG ATGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKR1D1 ( NM_005989.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005980.1 Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22861
Clone ID Related Accession (Same CDS sequence)	NM_005989.4 , NM_005989.3
Accession Version	NM_005989.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Homo sapiens(human)
Product	3-oxo-5-beta-steroid 4-dehydrogenase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298421.1, Z28339.1 and AC083867.7. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2010 this sequence version replaced NM_005989.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z28339.1, BC130625.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGCGA TGGAAGCTTG CAAAGACGCT 
GGCTTGGTGA AATCCCTGGG AGTGTCCAAT TTTAACCGCA GGCAGCTGGA GCTCATCCTG 
AACAAGCCAG GACTCAAACA CAAGCCAGTC AGCAACCAGG TTGAGTGCCA TCCGTATTTC 
ACCCAGCCAA AACTCTTGAA ATTTTGCCAA CAACATGACA TTGTCATTAC TGCATATAGC 
CCTTTGGGGA CCAGTAGGAA TCCAATCTGG GTGAATGTTT CTTCTCCACC TTTGTTAAAG 
GATGCACTTC TAAACTCATT GGGGAAAAGG TACAATAAGA CAGCAGCTCA AATTGTTTTG 
CGTTTCAACA TCCAGCGAGG GGTGGTTGTC ATTCCTAAAA GCTTTAATCT TGAAAGGATC 
AAAGAAAATT TTCAGATCTT TGACTTTTCT CTCACTGAAG AAGAAATGAA GGACATTGAA 
GCCTTGAATA AAAATGTCCG CTTTGTAGAA TTGCTCATGT GGCGCGATCA TCCTGAATAC 
CCATTTCATG ATGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005980.1
CDS	88..1068
Translation	MDLSAASHRIPLSDGNSIPIIGLGTYSEPKSTPKGACATSVKVAIDTGYRHIDGAYIYQNEHEVGEAIREKIAEGKVRREDIFYCGKLWATNHVPEMVRPTLERTLRVLQLDYVDLYIIEVPMAFKPGDEIYPRDENGKWLYHKSNLCATWEAMEACKDAGLVKSLGVSNFNRRQLELILNKPGLKHKPVSNQVECHPYFTQPKLLKFCQQHDIVITAYSPLGTSRNPIWVNVSSPPLLKDALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQIFDFSLTEEEMKDIEALNKNVRFVELLMWRDHPEYPFHDEY

Target ORF information:

RefSeq Version	NM_005989.3
Organism	Homo sapiens(human)
Definition	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005989.3

ORF Insert Sequence:

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGCGA TGGAAGCTTG CAAAGACGCT 
GGCTTGGTGA AATCCCTGGG AGTGTCCAAT TTTAACCGCA GGCAGCTGGA GCTCATCCTG 
AACAAGCCAG GACTCAAACA CAAGCCAGTC AGCAACCAGG TTGAGTGCCA TCCGTATTTC 
ACCCAGCCAA AACTCTTGAA ATTTTGCCAA CAACATGACA TTGTCATTAC TGCATATAGC 
CCTTTGGGGA CCAGTAGGAA TCCAATCTGG GTGAATGTTT CTTCTCCACC TTTGTTAAAG 
GATGCACTTC TAAACTCATT GGGGAAAAGG TACAATAAGA CAGCAGCTCA AATTGTTTTG 
CGTTTCAACA TCCAGCGAGG GGTGGTTGTC ATTCCTAAAA GCTTTAATCT TGAAAGGATC 
AAAGAAAATT TTCAGATCTT TGACTTTTCT CTCACTGAAG AAGAAATGAA GGACATTGAA 
GCCTTGAATA AAAATGTCCG CTTTGTAGAA TTGCTCATGT GGCGCGATCA TCCTGAATAC 
CCATTTCATG ATGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKR1D1 ( NM_001190906.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177835.1 Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 2, mRNA.

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CloneID	OHu22961
Clone ID Related Accession (Same CDS sequence)	NM_001190906.1 , NM_001190906.2
Accession Version	NM_001190906.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	3-oxo-5-beta-steroid 4-dehydrogenase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298421.1, AK298428.1 and AC083867.7. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298428.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGTTG AGTGCCATCC GTATTTCACC 
CAGCCAAAAC TCTTGAAATT TTGCCAACAA CATGACATTG TCATTACTGC ATATAGCCCT 
TTGGGGACCA GTAGGAATCC AATCTGGGTG AATGTTTCTT CTCCACCTTT GTTAAAGGAT 
GCACTTCTAA ACTCATTGGG GAAAAGGTAC AATAAGACAG CAGCTCAAAT TGTTTTGCGT 
TTCAACATCC AGCGAGGGGT GGTTGTCATT CCTAAAAGCT TTAATCTTGA AAGGATCAAA 
GAAAATTTTC AGATCTTTGA CTTTTCTCTC ACTGAAGAAG AAATGAAGGA CATTGAAGCC 
TTGAATAAAA ATGTCCGCTT TGTAGAATTG CTCATGTGGC GCGATCATCC TGAATACCCA 
TTTCATGATG AATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177835.1
CDS	88..945
Translation	MDLSAASHRIPLSDGNSIPIIGLGTYSEPKSTPKGACATSVKVAIDTGYRHIDGAYIYQNEHEVGEAIREKIAEGKVRREDIFYCGKLWATNHVPEMVRPTLERTLRVLQLDYVDLYIIEVPMAFKPGDEIYPRDENGKWLYHKSNLCATWEVECHPYFTQPKLLKFCQQHDIVITAYSPLGTSRNPIWVNVSSPPLLKDALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQIFDFSLTEEEMKDIEALNKNVRFVELLMWRDHPEYPFHDEY

Target ORF information:

RefSeq Version	NM_001190906.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190906.1

ORF Insert Sequence:

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGTTG AGTGCCATCC GTATTTCACC 
CAGCCAAAAC TCTTGAAATT TTGCCAACAA CATGACATTG TCATTACTGC ATATAGCCCT 
TTGGGGACCA GTAGGAATCC AATCTGGGTG AATGTTTCTT CTCCACCTTT GTTAAAGGAT 
GCACTTCTAA ACTCATTGGG GAAAAGGTAC AATAAGACAG CAGCTCAAAT TGTTTTGCGT 
TTCAACATCC AGCGAGGGGT GGTTGTCATT CCTAAAAGCT TTAATCTTGA AAGGATCAAA 
GAAAATTTTC AGATCTTTGA CTTTTCTCTC ACTGAAGAAG AAATGAAGGA CATTGAAGCC 
TTGAATAAAA ATGTCCGCTT TGTAGAATTG CTCATGTGGC GCGATCATCC TGAATACCCA 
TTTCATGATG AATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKR1D1 ( NM_001190906.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177835.1 Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22961
Clone ID Related Accession (Same CDS sequence)	NM_001190906.1 , NM_001190906.2
Accession Version	NM_001190906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	aldo-keto reductase family 1 member D1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298421.1, AK298428.1 and AC083867.7. On May 31, 2019 this sequence version replaced NM_001190906.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298428.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGTTG AGTGCCATCC GTATTTCACC 
CAGCCAAAAC TCTTGAAATT TTGCCAACAA CATGACATTG TCATTACTGC ATATAGCCCT 
TTGGGGACCA GTAGGAATCC AATCTGGGTG AATGTTTCTT CTCCACCTTT GTTAAAGGAT 
GCACTTCTAA ACTCATTGGG GAAAAGGTAC AATAAGACAG CAGCTCAAAT TGTTTTGCGT 
TTCAACATCC AGCGAGGGGT GGTTGTCATT CCTAAAAGCT TTAATCTTGA AAGGATCAAA 
GAAAATTTTC AGATCTTTGA CTTTTCTCTC ACTGAAGAAG AAATGAAGGA CATTGAAGCC 
TTGAATAAAA ATGTCCGCTT TGTAGAATTG CTCATGTGGC GCGATCATCC TGAATACCCA 
TTTCATGATG AATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177835.1
CDS	61..918
Translation	MDLSAASHRIPLSDGNSIPIIGLGTYSEPKSTPKGACATSVKVAIDTGYRHIDGAYIYQNEHEVGEAIREKIAEGKVRREDIFYCGKLWATNHVPEMVRPTLERTLRVLQLDYVDLYIIEVPMAFKPGDEIYPRDENGKWLYHKSNLCATWEVECHPYFTQPKLLKFCQQHDIVITAYSPLGTSRNPIWVNVSSPPLLKDALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQIFDFSLTEEEMKDIEALNKNVRFVELLMWRDHPEYPFHDEY

Target ORF information:

RefSeq Version	NM_001190906.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aldo-keto reductase family 1 member D1 (AKR1D1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190906.2

ORF Insert Sequence:

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC 
ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG 
GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT 
GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA 
GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA 
ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA 
GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG 
TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGTTG AGTGCCATCC GTATTTCACC 
CAGCCAAAAC TCTTGAAATT TTGCCAACAA CATGACATTG TCATTACTGC ATATAGCCCT 
TTGGGGACCA GTAGGAATCC AATCTGGGTG AATGTTTCTT CTCCACCTTT GTTAAAGGAT 
GCACTTCTAA ACTCATTGGG GAAAAGGTAC AATAAGACAG CAGCTCAAAT TGTTTTGCGT 
TTCAACATCC AGCGAGGGGT GGTTGTCATT CCTAAAAGCT TTAATCTTGA AAGGATCAAA 
GAAAATTTTC AGATCTTTGA CTTTTCTCTC ACTGAAGAAG AAATGAAGGA CATTGAAGCC 
TTGAATAAAA ATGTCCGCTT TGTAGAATTG CTCATGTGGC GCGATCATCC TGAATACCCA 
TTTCATGATG AATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dysregulation of ?
Molecular and cellular endocrinology(2017 Oct)
Valanejad L,Ghareeb M,Shiffka S,Nadolny C,Chen Y,Guo L,Verma R,You S,Akhlaghi F,Deng R

[Clinical feature and genetic analysis of a family affected by congenital bile acid synthesis defect type 2: identification of 2 novel mutations in AKR1D1 gene].
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics19(7)734-740(2017 Jul)
Cheng Y,Guo L,Deng M,Song YZ

In-Depth Dissection of the P133R Mutation in Steroid 5?-Reductase (AKR1D1): A Molecular Basis of Bile Acid Deficiency.
Biochemistry54(41)6343-51(2015 Oct)
Chen M,Jin Y,Penning TM

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AKR1D1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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