BPGM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BPGM
Entrez Gene ID	669
Full Name	bisphosphoglycerate mutase
Synonyms	DPGM
General protein information	Preferred Names bisphosphoglycerate mutase Names bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte 2,3-bisphosphoglycerate synthase 2,3-diphosphoglycerate mutase BPG-dependent PGAM erythrocyte 2,3-bisphosphoglycerate mutase testis secretory sperm-binding protein Li 202a
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q33
Summary	2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009].
Disorder MIM:	613896
Disorder Html:	Erythrocytosis due to bisphosphoglycerate mutase deficiency, 222800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001293085.1	NP_001280014.1	bisphosphoglycerate mutase
XM_011516527.2	XP_011514829.1	bisphosphoglycerate mutase isoform X1
NM_001724.5	NP_001715.1	bisphosphoglycerate mutase
XM_011516527.1	XP_011514829.1	bisphosphoglycerate mutase isoform X1
NM_001724.4	NP_001715.1	bisphosphoglycerate mutase
NM_199186.2	NP_954655.1	bisphosphoglycerate mutase

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BPGM	NP_001025939.1
Bos taurus (cattle)	BPGM	NP_001030479.1
Rattus norvegicus (Norway rat)	Bpgm	NP_955414.1
	BPGM	XP_001102701.1
Mus musculus (house mouse)	Bpgm	NP_031589.1
Danio rerio (zebrafish)	bpgm	NP_001002630.1
Xenopus tropicalis (tropical clawed frog)	bpgm	NP_001016599.1
Homo sapiens (human)	BPGM	NP_001715.1
Pan troglodytes (chimpanzee)	BPGM	XP_001141002.1
Canis lupus familiaris (dog)	BPGM	XP_005629661.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Unliganded structure of human bisphosphoglycerate mutase reveals side-chain movements induced by ligand binding.
Patterson A, Price NC, Nairn J
Acta crystallographica. Section F, Structural biology and crystallization communications66(Pt 11)1415-20(2010 Nov)

Seeing the process of histidine phosphorylation in human bisphosphoglycerate mutase.
Wang Y, Liu L, Wei Z, Cheng Z, Lin Y, Gong W
The Journal of biological chemistry281(51)39642-8(2006 Dec)

Crystal structure of human bisphosphoglycerate mutase.
Wang Y, Wei Z, Bian Q, Cheng Z, Wan M, Liu L, Gong W
The Journal of biological chemistry279(37)39132-8(2004 Sep)

Human erythrocyte bisphosphoglycerate mutase: inactivation by glycation in vivo and in vitro.
Fujita T, Suzuki K, Tada T, Yoshihara Y, Hamaoka R, Uchida K, Matuo Y, Sasaki T, Hanafusa T, Taniguchi N
Journal of biochemistry124(6)1237-44(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

1.94 A resolution X-ray structure of bisphosphoglycerate mutase is presented, focusing on the dynamic nature of key ligand-binding residues and their interaction with the inhibitor citrate
Title: Unliganded structure of human bisphosphoglycerate mutase reveals side-chain movements induced by ligand binding.

analysis of histidine phosphorylation in human bisphosphoglycerate mutase
Title: Seeing the process of histidine phosphorylation in human bisphosphoglycerate mutase.

bisphosphoglycerate mutase crystallographic structure
Title: Crystal structure of human bisphosphoglycerate mutase.

The following BPGM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BPGM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27712	NM_199186.2 Latest version!	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27712	NM_001724.5 Latest version!	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27712	NM_001293085.1 Latest version!	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27712	XM_011516527.2 Latest version!	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu27712	XM_011516527.1	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27712	NM_001724.4	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BPGM ( NM_001293085.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280014.1 Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27712
Clone ID Related Accession (Same CDS sequence)	NM_001293085.1 , XM_011516527.2 , NM_001724.5 , XM_011516527.1 , NM_001724.4 , NM_199186.2
Accession Version	NM_001293085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	bisphosphoglycerate mutase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB047822.1, DA701935.1, BC017050.1 and BU608668.1. On May 28, 2014 this sequence version replaced XM_006716096.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 2. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: HM005615.1, SRR1163655.665707.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280014.1
CDS	346..1125
Translation	MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK

Target ORF information:

RefSeq Version	NM_001293085.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293085.1

ORF Insert Sequence:

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BPGM ( NM_001724.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001715.1 Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27712
Clone ID Related Accession (Same CDS sequence)	NM_001293085.1 , XM_011516527.2 , NM_001724.5 , XM_011516527.1 , NM_001724.4 , NM_199186.2
Accession Version	NM_001724.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	bisphosphoglycerate mutase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC017050.1 and BU608668.1. On Nov 23, 2018 this sequence version replaced NM_001724.4. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005615.1, SRR3476690.1129680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000344924.8/ ENSP00000342032.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001715.1
CDS	147..926
Translation	MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK

Target ORF information:

RefSeq Version	NM_001724.5
Organism	Homo sapiens(human)
Definition	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001724.5

ORF Insert Sequence:

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BPGM ( NM_001724.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001715.1 Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27712
Clone ID Related Accession (Same CDS sequence)	NM_001293085.1 , XM_011516527.2 , NM_001724.5 , XM_011516527.1 , NM_001724.4 , NM_199186.2
Accession Version	NM_001724.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	bisphosphoglycerate mutase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB047822.1, BC017050.1 and BU608668.1. On Feb 19, 2009 this sequence version replaced NM_001724.3. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005615.1, SRR1803611.196828.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001715.1
CDS	221..1000
Translation	MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK

Target ORF information:

RefSeq Version	NM_001724.4
Organism	Homo sapiens(human)
Definition	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001724.4

ORF Insert Sequence:

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BPGM ( NM_199186.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_954655.1 Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27712
Clone ID Related Accession (Same CDS sequence)	NM_001293085.1 , XM_011516527.2 , NM_001724.5 , XM_011516527.1 , NM_001724.4 , NM_199186.2
Accession Version	NM_199186.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	bisphosphoglycerate mutase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB047822.1, DB049554.1, BC017050.1 and BU608668.1. This sequence is a reference standard in the RefSeqGene project. On Feb 19, 2009 this sequence version replaced NM_199186.1. Transcript Variant: This variant (2) represents the longer transcript. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.242556.1, SRR1660803.11458.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954655.1
CDS	542..1321
Translation	MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK

Target ORF information:

RefSeq Version	NM_199186.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199186.2

ORF Insert Sequence:

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BPGM ( XM_011516527.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514829.1 Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu27712
Clone ID Related Accession (Same CDS sequence)	NM_001293085.1 , XM_011516527.2 , NM_001724.5 , XM_011516527.1 , NM_001724.4 , NM_199186.2
Accession Version	XM_011516527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bisphosphoglycerate mutase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011516527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514829.1
CDS	402..1181
Translation	MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK

Target ORF information:

RefSeq Version	XM_011516527.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516527.2

ORF Insert Sequence:

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BPGM ( XM_011516527.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514829.1 Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu27712
Clone ID Related Accession (Same CDS sequence)	NM_001293085.1 , XM_011516527.2 , NM_001724.5 , XM_011516527.1 , NM_001724.4 , NM_199186.2
Accession Version	XM_011516527.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bisphosphoglycerate mutase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514829.1
CDS	402..1181
Translation	MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK

Target ORF information:

RefSeq Version	XM_011516527.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bisphosphoglycerate mutase (BPGM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516527.1

ORF Insert Sequence:

ATGTCCAAGT ACAAACTTAT TATGTTAAGA CATGGAGAGG GTGCTTGGAA TAAGGAGAAC 
CGTTTTTGTA GCTGGGTGGA TCAGAAACTC AACAGCGAAG GAATGGAGGA AGCTCGGAAC 
TGTGGGAAGC AACTCAAAGC GTTAAACTTT GAGTTTGATC TTGTATTCAC ATCTGTCCTT 
AATCGGTCCA TTCACACAGC CTGGCTGATC CTGGAAGAGC TAGGCCAGGA ATGGGTGCCT 
GTGGAAAGCT CCTGGCGTCT AAATGAGCGT CACTATGGGG CCTTGATCGG TCTCAACAGG 
GAGCAGATGG CTTTGAATCA TGGTGAAGAA CAAGTGAGGC TCTGGAGAAG AAGCTACAAT 
GTAACCCCGC CTCCCATTGA GGAGTCTCAT CCTTACTACC AAGAAATCTA CAACGACCGG 
AGGTATAAAG TATGCGATGT GCCCTTGGAT CAACTGCCAC GGTCGGAAAG CTTAAAGGAT 
GTTCTGGAGA GACTCCTTCC CTATTGGAAT GAAAGGATTG CTCCCGAAGT ATTACGTGGC 
AAAACCATTC TGATATCTGC TCATGGAAAT AGCAGTAGGG CACTCCTAAA ACACCTGGAA 
GGTATCTCAG ATGAAGACAT CATCAACATT ACTCTTCCTA CTGGAGTCCC CATTCTTCTG 
GAATTGGATG AAAACCTGCG TGCTGTTGGG CCTCATCAGT TCCTGGGTGA CCAAGAGGCG 
ATCCAAGCAG CCATTAAGAA AGTAGAAGAT CAAGGAAAAG TGAAACAAGC TAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Unliganded structure of human bisphosphoglycerate mutase reveals side-chain movements induced by ligand binding.
Acta crystallographica. Section F, Structural biology and crystallization communications66(Pt 11)1415-20(2010 Nov)
Patterson A,Price NC,Nairn J

Seeing the process of histidine phosphorylation in human bisphosphoglycerate mutase.
The Journal of biological chemistry281(51)39642-8(2006 Dec)
Wang Y,Liu L,Wei Z,Cheng Z,Lin Y,Gong W

Crystal structure of human bisphosphoglycerate mutase.
The Journal of biological chemistry279(37)39132-8(2004 Sep)
Wang Y,Wei Z,Bian Q,Cheng Z,Wan M,Liu L,Gong W

Human erythrocyte bisphosphoglycerate mutase: inactivation by glycation in vivo and in vitro.
Journal of biochemistry124(6)1237-44(1998 Dec)
Fujita T,Suzuki K,Tada T,Yoshihara Y,Hamaoka R,Uchida K,Matuo Y,Sasaki T,Hanafusa T,Taniguchi N

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BPGM cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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