SNRPB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SNRPB
Entrez Gene ID	6628
Full Name	small nuclear ribonucleoprotein polypeptides B and B1
Synonyms	CCMS,COD,SNRPB1,Sm-B/B',SmB/B',SmB/SmB',snRNP-B
General protein information	Preferred Names small nuclear ribonucleoprotein polypeptides B and B1 Names small nuclear ribonucleoprotein-associated proteins B and B' B polypeptide of Sm protein Sm protein B/B' sm-B/Sm-B' small nuclear ribonucleoprotein polypeptide B small nuclear ribonucleoprotein polypeptides B and B'
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20p13
Summary	The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	182282
Disorder Html:	Cerebrocostomandibular syndrome, 117650 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_198216.2	NP_937859.1	small nuclear ribonucleoprotein-associated proteins B and B' isoform B'
NM_003091.3	NP_003082.1	small nuclear ribonucleoprotein-associated proteins B and B' isoform B
NM_003091.4	NP_003082.1	small nuclear ribonucleoprotein-associated proteins B and B' isoform B
NM_198216.1	NP_937859.1	small nuclear ribonucleoprotein-associated proteins B and B' isoform B'

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SNRPB	XP_861671.1
Homo sapiens (human)	SNRPB	NP_937859.1
Bos taurus (cattle)	SNRPB	NP_001029903.1
Mus musculus (house mouse)	Snrpb	NP_033251.1
Pan troglodytes (chimpanzee)	SNRPB	XP_525398.2
	SNRPB	XP_001113951.1
Rattus norvegicus (Norway rat)	Snrpb	NP_599185.1

Gene Ontology
Bibliography

Related articles in PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, L?hrmann R, Stark H
Cell170(4)701-713.e11(2017 Aug)

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, L?hrmann R
Nature542(7641)318-323(2017 02)

Functional genomics analyses of RNA-binding proteins reveal the splicing regulator SNRPB as an oncogenic candidate in glioblastoma.
Correa BR, de Araujo PR, Qiao M, Burns SC, Chen C, Schlegel R, Agarwal S, Galante PA, Penalva LO
Genome biology17(1)125(2016 Jun)

Cerebro-costo-mandibular syndrome: Clinical, radiological, and genetic findings.
Tooley M, Lynch D, Bernier F, Parboosingh J, Bhoj E, Zackai E, Calder A, Itasaki N, Wakeling E, Scott R, Lees M, Clayton-Smith J, Blyth M, Morton J, Shears D, Kini U, Homfray T, Clarke A, Barnicoat A, Wallis C, Hewitson R, Offiah A, Saunders M, Langton-Hewer S, Hilliard T, Davis P, Smithson S
American journal of medical genetics. Part A170A(5)1115-26(2016 May)

Molecular architecture of the human U4/U6.U5 tri-snRNP.
Agafonov DE, Kastner B, Dybkov O, Hofele RV, Liu WT, Urlaub H, L?hrmann R, Stark H
Science (New York, N.Y.)351(6280)1416-20(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our study provides new insights into how RBPs, and specifically SNRPB, regulate gene expression and directly impact GBM development.
Title: Functional genomics analyses of RNA-binding proteins reveal the splicing regulator SNRPB as an oncogenic candidate in glioblastoma.

These results confirm the specificity of SNRPB mutations in CCMS and provide further evidence for the role of spliceosomal proteins in craniofacial and thoracic development.
Title: Cerebro-costo-mandibular syndrome: Clinical, radiological, and genetic findings.

Cerebro-costo-mandibular syndrome is due to heterozygous mutations in SNRPB.
Title: Mutations in SNRPB, encoding components of the core splicing machinery, cause cerebro-costo-mandibular syndrome.

Mutations in the regulatory alternative exon of SNRPB disrupted it's auto-regulation and caused cerebro-costo-mandibular syndrome.
Title: Disrupted auto-regulation of the spliceosomal gene SNRPB causes cerebro-costo-mandibular syndrome.

Highly mobile SmB protein-trafficking vesicles in neural cells are dependent on the cellular levels of SMN and SmB for their morphology and mobility.
Title: Time-resolved quantitative proteomics implicates the core snRNP protein SmB together with SMN in neural trafficking.

The following SNRPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SNRPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02514	NM_198216.2 Latest version!	Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu04064	NM_003091.4 Latest version!	Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu04064	NM_003091.3	Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu02514	NM_198216.1	Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SNRPB ( NM_198216.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_937859.1 Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02514
Clone ID Related Accession (Same CDS sequence)	NM_198216.2 , NM_198216.1
Accession Version	NM_198216.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein-associated proteins B and B' isoform B'
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM559678.1 and X17568.1. On Jun 2, 2019 this sequence version replaced NM_198216.1. Transcript Variant: This variant (1) encodes the longer isoform (B'). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.584125.1, SRR1163658.213972.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGG GCAAGAGCAG CAAGATGCTG CAGCATATTG ATTACAGGAT GAGGTGCATC 
CTGCAGGACG GCCGGATCTT CATTGGCACC TTCAAGGCTT TTGACAAGCA CATGAATTTG 
ATCCTCTGTG ACTGTGATGA GTTCAGAAAG ATCAAGCCAA AGAACTCCAA ACAAGCAGAA 
AGGGAAGAGA AGCGAGTCCT CGGTCTGGTG CTGCTGCGAG GGGAGAATCT GGTCTCAATG 
ACAGTAGAGG GACCTCCTCC CAAAGATACT GGTATTGCTC GAGTTCCACT TGCTGGAGCT 
GCCGGGGGCC CAGGGATCGG CAGGGCTGCT GGCAGAGGAA TCCCAGCTGG GGTTCCCATG 
CCCCAGGCTC CTGCAGGACT TGCTGGGCCA GTCCGTGGGG TTGGCGGGCC ATCCCAACAG 
GTGATGACCC CACAAGGAAG AGGTACTGTT GCAGCCGCTG CAGCTGCTGC CACAGCCAGT 
ATTGCCGGGG CTCCAACCCA GTACCCACCT GGCCGTGGGG GTCCTCCCCC ACCTATGGGC 
CGAGGAGCAC CCCCTCCAGG CATGATGGGC CCACCTCCTG GTATGAGACC TCCTATGGGT 
CCCCCAATGG GGATCCCCCC TGGAAGAGGG ACTCCAATGG GCATGCCCCC TCCGGGAATG 
CGGCCTCCTC CCCCTGGGAT GCGAGGGCCC CCTCCCCCGG GAATGCGCCC ACCAAGGCCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937859.1
CDS	100..822
Translation	MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPMPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMGRGAPPPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGPPPPGMRPPRP

Target ORF information:

RefSeq Version	NM_198216.2
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198216.2

ORF Insert Sequence:

ATGACGGTGG GCAAGAGCAG CAAGATGCTG CAGCATATTG ATTACAGGAT GAGGTGCATC 
CTGCAGGACG GCCGGATCTT CATTGGCACC TTCAAGGCTT TTGACAAGCA CATGAATTTG 
ATCCTCTGTG ACTGTGATGA GTTCAGAAAG ATCAAGCCAA AGAACTCCAA ACAAGCAGAA 
AGGGAAGAGA AGCGAGTCCT CGGTCTGGTG CTGCTGCGAG GGGAGAATCT GGTCTCAATG 
ACAGTAGAGG GACCTCCTCC CAAAGATACT GGTATTGCTC GAGTTCCACT TGCTGGAGCT 
GCCGGGGGCC CAGGGATCGG CAGGGCTGCT GGCAGAGGAA TCCCAGCTGG GGTTCCCATG 
CCCCAGGCTC CTGCAGGACT TGCTGGGCCA GTCCGTGGGG TTGGCGGGCC ATCCCAACAG 
GTGATGACCC CACAAGGAAG AGGTACTGTT GCAGCCGCTG CAGCTGCTGC CACAGCCAGT 
ATTGCCGGGG CTCCAACCCA GTACCCACCT GGCCGTGGGG GTCCTCCCCC ACCTATGGGC 
CGAGGAGCAC CCCCTCCAGG CATGATGGGC CCACCTCCTG GTATGAGACC TCCTATGGGT 
CCCCCAATGG GGATCCCCCC TGGAAGAGGG ACTCCAATGG GCATGCCCCC TCCGGGAATG 
CGGCCTCCTC CCCCTGGGAT GCGAGGGCCC CCTCCCCCGG GAATGCGCCC ACCAAGGCCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPB ( NM_198216.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_937859.1 Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02514
Clone ID Related Accession (Same CDS sequence)	NM_198216.2 , NM_198216.1
Accession Version	NM_198216.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein-associated proteins B and B' isoform B'
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM559678.1 and X17568.1. Transcript Variant: This variant (1) encodes the longer isoform (B'). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X17568.1, SRR1163657.273839.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGG GCAAGAGCAG CAAGATGCTG CAGCATATTG ATTACAGGAT GAGGTGCATC 
CTGCAGGACG GCCGGATCTT CATTGGCACC TTCAAGGCTT TTGACAAGCA CATGAATTTG 
ATCCTCTGTG ACTGTGATGA GTTCAGAAAG ATCAAGCCAA AGAACTCCAA ACAAGCAGAA 
AGGGAAGAGA AGCGAGTCCT CGGTCTGGTG CTGCTGCGAG GGGAGAATCT GGTCTCAATG 
ACAGTAGAGG GACCTCCTCC CAAAGATACT GGTATTGCTC GAGTTCCACT TGCTGGAGCT 
GCCGGGGGCC CAGGGATCGG CAGGGCTGCT GGCAGAGGAA TCCCAGCTGG GGTTCCCATG 
CCCCAGGCTC CTGCAGGACT TGCTGGGCCA GTCCGTGGGG TTGGCGGGCC ATCCCAACAG 
GTGATGACCC CACAAGGAAG AGGTACTGTT GCAGCCGCTG CAGCTGCTGC CACAGCCAGT 
ATTGCCGGGG CTCCAACCCA GTACCCACCT GGCCGTGGGG GTCCTCCCCC ACCTATGGGC 
CGAGGAGCAC CCCCTCCAGG CATGATGGGC CCACCTCCTG GTATGAGACC TCCTATGGGT 
CCCCCAATGG GGATCCCCCC TGGAAGAGGG ACTCCAATGG GCATGCCCCC TCCGGGAATG 
CGGCCTCCTC CCCCTGGGAT GCGAGGGCCC CCTCCCCCGG GAATGCGCCC ACCAAGGCCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937859.1
CDS	164..886
Translation	MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPMPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMGRGAPPPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGPPPPGMRPPRP

Target ORF information:

RefSeq Version	NM_198216.1
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198216.1

ORF Insert Sequence:

ATGACGGTGG GCAAGAGCAG CAAGATGCTG CAGCATATTG ATTACAGGAT GAGGTGCATC 
CTGCAGGACG GCCGGATCTT CATTGGCACC TTCAAGGCTT TTGACAAGCA CATGAATTTG 
ATCCTCTGTG ACTGTGATGA GTTCAGAAAG ATCAAGCCAA AGAACTCCAA ACAAGCAGAA 
AGGGAAGAGA AGCGAGTCCT CGGTCTGGTG CTGCTGCGAG GGGAGAATCT GGTCTCAATG 
ACAGTAGAGG GACCTCCTCC CAAAGATACT GGTATTGCTC GAGTTCCACT TGCTGGAGCT 
GCCGGGGGCC CAGGGATCGG CAGGGCTGCT GGCAGAGGAA TCCCAGCTGG GGTTCCCATG 
CCCCAGGCTC CTGCAGGACT TGCTGGGCCA GTCCGTGGGG TTGGCGGGCC ATCCCAACAG 
GTGATGACCC CACAAGGAAG AGGTACTGTT GCAGCCGCTG CAGCTGCTGC CACAGCCAGT 
ATTGCCGGGG CTCCAACCCA GTACCCACCT GGCCGTGGGG GTCCTCCCCC ACCTATGGGC 
CGAGGAGCAC CCCCTCCAGG CATGATGGGC CCACCTCCTG GTATGAGACC TCCTATGGGT 
CCCCCAATGG GGATCCCCCC TGGAAGAGGG ACTCCAATGG GCATGCCCCC TCCGGGAATG 
CGGCCTCCTC CCCCTGGGAT GCGAGGGCCC CCTCCCCCGG GAATGCGCCC ACCAAGGCCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPB ( NM_003091.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003082.1 Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04064
Clone ID Related Accession (Same CDS sequence)	NM_003091.3 , NM_003091.4
Accession Version	NM_003091.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein-associated proteins B and B' isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM559678.1 and X17567.1. This sequence is a reference standard in the RefSeqGene project. On Nov 3, 2003 this sequence version replaced NM_003091.2. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, that causes a frameshift. The resulting isoform (B) is shorter and has a distinct C-terminus as compared to isoform B'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.568942.1, SRR1163657.421629.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGG GCAAGAGCAG CAAGATGCTG CAGCATATTG ATTACAGGAT GAGGTGCATC 
CTGCAGGACG GCCGGATCTT CATTGGCACC TTCAAGGCTT TTGACAAGCA CATGAATTTG 
ATCCTCTGTG ACTGTGATGA GTTCAGAAAG ATCAAGCCAA AGAACTCCAA ACAAGCAGAA 
AGGGAAGAGA AGCGAGTCCT CGGTCTGGTG CTGCTGCGAG GGGAGAATCT GGTCTCAATG 
ACAGTAGAGG GACCTCCTCC CAAAGATACT GGTATTGCTC GAGTTCCACT TGCTGGAGCT 
GCCGGGGGCC CAGGGATCGG CAGGGCTGCT GGCAGAGGAA TCCCAGCTGG GGTTCCCATG 
CCCCAGGCTC CTGCAGGACT TGCTGGGCCA GTCCGTGGGG TTGGCGGGCC ATCCCAACAG 
GTGATGACCC CACAAGGAAG AGGTACTGTT GCAGCCGCTG CAGCTGCTGC CACAGCCAGT 
ATTGCCGGGG CTCCAACCCA GTACCCACCT GGCCGTGGGG GTCCTCCCCC ACCTATGGGC 
CGAGGAGCAC CCCCTCCAGG CATGATGGGC CCACCTCCTG GTATGAGACC TCCTATGGGT 
CCCCCAATGG GGATCCCCCC TGGAAGAGGG ACTCCAATGG GCATGCCCCC TCCGGGAATG 
CGGCCTCCTC CCCCTGGGAT GCGAGGCCTT CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003082.1
CDS	164..859
Translation	MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPMPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMGRGAPPPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGLL

Target ORF information:

RefSeq Version	NM_003091.3
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003091.3

ORF Insert Sequence:

ATGACGGTGG GCAAGAGCAG CAAGATGCTG CAGCATATTG ATTACAGGAT GAGGTGCATC 
CTGCAGGACG GCCGGATCTT CATTGGCACC TTCAAGGCTT TTGACAAGCA CATGAATTTG 
ATCCTCTGTG ACTGTGATGA GTTCAGAAAG ATCAAGCCAA AGAACTCCAA ACAAGCAGAA 
AGGGAAGAGA AGCGAGTCCT CGGTCTGGTG CTGCTGCGAG GGGAGAATCT GGTCTCAATG 
ACAGTAGAGG GACCTCCTCC CAAAGATACT GGTATTGCTC GAGTTCCACT TGCTGGAGCT 
GCCGGGGGCC CAGGGATCGG CAGGGCTGCT GGCAGAGGAA TCCCAGCTGG GGTTCCCATG 
CCCCAGGCTC CTGCAGGACT TGCTGGGCCA GTCCGTGGGG TTGGCGGGCC ATCCCAACAG 
GTGATGACCC CACAAGGAAG AGGTACTGTT GCAGCCGCTG CAGCTGCTGC CACAGCCAGT 
ATTGCCGGGG CTCCAACCCA GTACCCACCT GGCCGTGGGG GTCCTCCCCC ACCTATGGGC 
CGAGGAGCAC CCCCTCCAGG CATGATGGGC CCACCTCCTG GTATGAGACC TCCTATGGGT 
CCCCCAATGG GGATCCCCCC TGGAAGAGGG ACTCCAATGG GCATGCCCCC TCCGGGAATG 
CGGCCTCCTC CCCCTGGGAT GCGAGGCCTT CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPB ( NM_003091.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003082.1 Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04064
Clone ID Related Accession (Same CDS sequence)	NM_003091.3 , NM_003091.4
Accession Version	NM_003091.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein-associated proteins B and B' isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM559678.1 and X17567.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_003091.3. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, that causes a frameshift. The resulting isoform (B) is shorter and has a distinct C-terminus as compared to isoform B'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.485878.1, SRR1163658.208409.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGG GCAAGAGCAG CAAGATGCTG CAGCATATTG ATTACAGGAT GAGGTGCATC 
CTGCAGGACG GCCGGATCTT CATTGGCACC TTCAAGGCTT TTGACAAGCA CATGAATTTG 
ATCCTCTGTG ACTGTGATGA GTTCAGAAAG ATCAAGCCAA AGAACTCCAA ACAAGCAGAA 
AGGGAAGAGA AGCGAGTCCT CGGTCTGGTG CTGCTGCGAG GGGAGAATCT GGTCTCAATG 
ACAGTAGAGG GACCTCCTCC CAAAGATACT GGTATTGCTC GAGTTCCACT TGCTGGAGCT 
GCCGGGGGCC CAGGGATCGG CAGGGCTGCT GGCAGAGGAA TCCCAGCTGG GGTTCCCATG 
CCCCAGGCTC CTGCAGGACT TGCTGGGCCA GTCCGTGGGG TTGGCGGGCC ATCCCAACAG 
GTGATGACCC CACAAGGAAG AGGTACTGTT GCAGCCGCTG CAGCTGCTGC CACAGCCAGT 
ATTGCCGGGG CTCCAACCCA GTACCCACCT GGCCGTGGGG GTCCTCCCCC ACCTATGGGC 
CGAGGAGCAC CCCCTCCAGG CATGATGGGC CCACCTCCTG GTATGAGACC TCCTATGGGT 
CCCCCAATGG GGATCCCCCC TGGAAGAGGG ACTCCAATGG GCATGCCCCC TCCGGGAATG 
CGGCCTCCTC CCCCTGGGAT GCGAGGCCTT CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003082.1
CDS	100..795
Translation	MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPMPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMGRGAPPPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGLL

Target ORF information:

RefSeq Version	NM_003091.4
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003091.4

ORF Insert Sequence:

ATGACGGTGG GCAAGAGCAG CAAGATGCTG CAGCATATTG ATTACAGGAT GAGGTGCATC 
CTGCAGGACG GCCGGATCTT CATTGGCACC TTCAAGGCTT TTGACAAGCA CATGAATTTG 
ATCCTCTGTG ACTGTGATGA GTTCAGAAAG ATCAAGCCAA AGAACTCCAA ACAAGCAGAA 
AGGGAAGAGA AGCGAGTCCT CGGTCTGGTG CTGCTGCGAG GGGAGAATCT GGTCTCAATG 
ACAGTAGAGG GACCTCCTCC CAAAGATACT GGTATTGCTC GAGTTCCACT TGCTGGAGCT 
GCCGGGGGCC CAGGGATCGG CAGGGCTGCT GGCAGAGGAA TCCCAGCTGG GGTTCCCATG 
CCCCAGGCTC CTGCAGGACT TGCTGGGCCA GTCCGTGGGG TTGGCGGGCC ATCCCAACAG 
GTGATGACCC CACAAGGAAG AGGTACTGTT GCAGCCGCTG CAGCTGCTGC CACAGCCAGT 
ATTGCCGGGG CTCCAACCCA GTACCCACCT GGCCGTGGGG GTCCTCCCCC ACCTATGGGC 
CGAGGAGCAC CCCCTCCAGG CATGATGGGC CCACCTCCTG GTATGAGACC TCCTATGGGT 
CCCCCAATGG GGATCCCCCC TGGAAGAGGG ACTCCAATGG GCATGCCCCC TCCGGGAATG 
CGGCCTCCTC CCCCTGGGAT GCGAGGCCTT CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Cell170(4)701-713.e11(2017 Aug)
Bertram K,Agafonov DE,Dybkov O,Haselbach D,Leelaram MN,Will CL,Urlaub H,Kastner B,L?hrmann R,Stark H

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Nature542(7641)318-323(2017 02)
Bertram K,Agafonov DE,Liu WT,Dybkov O,Will CL,Hartmuth K,Urlaub H,Kastner B,Stark H,L?hrmann R

Functional genomics analyses of RNA-binding proteins reveal the splicing regulator SNRPB as an oncogenic candidate in glioblastoma.
Genome biology17(1)125(2016 Jun)
Correa BR,de Araujo PR,Qiao M,Burns SC,Chen C,Schlegel R,Agarwal S,Galante PA,Penalva LO

Cerebro-costo-mandibular syndrome: Clinical, radiological, and genetic findings.
American journal of medical genetics. Part A170A(5)1115-26(2016 May)
Tooley M,Lynch D,Bernier F,Parboosingh J,Bhoj E,Zackai E,Calder A,Itasaki N,Wakeling E,Scott R,Lees M,Clayton-Smith J,Blyth M,Morton J,Shears D,Kini U,Homfray T,Clarke A,Barnicoat A,Wallis C,Hewitson R,Offiah A,Saunders M,Langton-Hewer S,Hilliard T,Davis P,Smithson S

Molecular architecture of the human U4/U6.U5 tri-snRNP.
Science (New York, N.Y.)351(6280)1416-20(2016 Mar)
Agafonov DE,Kastner B,Dybkov O,Hofele RV,Liu WT,Urlaub H,L?hrmann R,Stark H

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SNRPB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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