SMPD1 cDNA ORF clone, Homo sapiens(human)

The following SMPD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMPD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18710 NM_000543.5
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu60647 NM_001365135.2
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu60647 XM_011520303.1
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu60648 XM_011520304.2
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu101203 NM_001318088.2
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu16724 NM_001007593.3
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu44952 NM_001318087.2
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
View Old Accession Versions >>
OHu18710 NM_000543.4 Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu60647 NM_001365135.1 Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu60648 XM_011520304.1 Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu44952 NM_001318087.1 Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu16724 NM_001007593.2 Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu101203 NM_001318088.1 Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu18710
    Clone ID Related Accession (Same CDS sequence) NM_000543.4 , NM_000543.5
    Accession Version NM_000543.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, AB209775.1, M59916.1 and AA746054.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2010 this sequence version replaced NM_000543.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209775.1, X59960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
    TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
    CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
    AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
    TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
    CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
    GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGGTTA CCGTGTGTAC
    CAAATAGATG GAAACTACTC CGGGAGCTCT CACGTGGTCC TGGACCATGA GACCTACATC
    CTGAATCTGA CCCAGGCAAA CATACCGGGA GCCATACCGC ACTGGCAGCT TCTCTACAGG
    GCTCGAGAAA CCTATGGGCT GCCCAACACA CTGCCTACCG CCTGGCACAA CCTGGTATAT
    CGCATGCGGG GCGACATGCA ACTTTTCCAG ACCTTCTGGT TTCTCTACCA TAAGGGCCAC
    CCACCCTCGG AGCCCTGTGG CACGCCCTGC CGTCTGGCTA CTCTTTGTGC CCAGCTCTCT
    GCCCGTGCTG ACAGCCCTGC TCTGTGCCGC CACCTGATGC CAGATGGGAG CCTCCCAGAG
    GCCCAGAGCC TGTGGCCAAG GCCACTGTTT TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000534.3
    CDS186..2081
    Translation

    Target ORF information:

    RefSeq Version NM_000543.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000543.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
    TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
    CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
    AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
    TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
    CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
    GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGGTTA CCGTGTGTAC
    CAAATAGATG GAAACTACTC CGGGAGCTCT CACGTGGTCC TGGACCATGA GACCTACATC
    CTGAATCTGA CCCAGGCAAA CATACCGGGA GCCATACCGC ACTGGCAGCT TCTCTACAGG
    GCTCGAGAAA CCTATGGGCT GCCCAACACA CTGCCTACCG CCTGGCACAA CCTGGTATAT
    CGCATGCGGG GCGACATGCA ACTTTTCCAG ACCTTCTGGT TTCTCTACCA TAAGGGCCAC
    CCACCCTCGG AGCCCTGTGG CACGCCCTGC CGTCTGGCTA CTCTTTGTGC CCAGCTCTCT
    GCCCGTGCTG ACAGCCCTGC TCTGTGCCGC CACCTGATGC CAGATGGGAG CCTCCCAGAG
    GCCCAGAGCC TGTGGCCAAG GCCACTGTTT TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18710
    Clone ID Related Accession (Same CDS sequence) NM_000543.4 , NM_000543.5
    Accession Version NM_000543.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB209775.1, M59916.1 and AA746054.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000543.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.126536.1, AB209775.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000342245.9/ ENSP00000340409.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
    TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
    CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
    AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
    TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
    CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
    GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGGTTA CCGTGTGTAC
    CAAATAGATG GAAACTACTC CGGGAGCTCT CACGTGGTCC TGGACCATGA GACCTACATC
    CTGAATCTGA CCCAGGCAAA CATACCGGGA GCCATACCGC ACTGGCAGCT TCTCTACAGG
    GCTCGAGAAA CCTATGGGCT GCCCAACACA CTGCCTACCG CCTGGCACAA CCTGGTATAT
    CGCATGCGGG GCGACATGCA ACTTTTCCAG ACCTTCTGGT TTCTCTACCA TAAGGGCCAC
    CCACCCTCGG AGCCCTGTGG CACGCCCTGC CGTCTGGCTA CTCTTTGTGC CCAGCTCTCT
    GCCCGTGCTG ACAGCCCTGC TCTGTGCCGC CACCTGATGC CAGATGGGAG CCTCCCAGAG
    GCCCAGAGCC TGTGGCCAAG GCCACTGTTT TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000534.3
    CDS126..2021
    Translation

    Target ORF information:

    RefSeq Version NM_000543.5
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000543.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
    TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
    CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
    AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
    TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
    CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
    GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGGTTA CCGTGTGTAC
    CAAATAGATG GAAACTACTC CGGGAGCTCT CACGTGGTCC TGGACCATGA GACCTACATC
    CTGAATCTGA CCCAGGCAAA CATACCGGGA GCCATACCGC ACTGGCAGCT TCTCTACAGG
    GCTCGAGAAA CCTATGGGCT GCCCAACACA CTGCCTACCG CCTGGCACAA CCTGGTATAT
    CGCATGCGGG GCGACATGCA ACTTTTCCAG ACCTTCTGGT TTCTCTACCA TAAGGGCCAC
    CCACCCTCGG AGCCCTGTGG CACGCCCTGC CGTCTGGCTA CTCTTTGTGC CCAGCTCTCT
    GCCCGTGCTG ACAGCCCTGC TCTGTGCCGC CACCTGATGC CAGATGGGAG CCTCCCAGAG
    GCCCAGAGCC TGTGGCCAAG GCCACTGTTT TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60647
    Clone ID Related Accession (Same CDS sequence) NM_001365135.1 , NM_001365135.2 , XM_011520303.1
    Accession Version NM_001365135.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-28
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068733.12. On Jul 31, 2018 this sequence version replaced XM_011520303.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.665950.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
    ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
    GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
    TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
    CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGGTTACC GTGTGTACCA AATAGATGGA
    AACTACTCCG GGAGCTCTCA CGTGGTCCTG GACCATGAGA CCTACATCCT GAATCTGACC
    CAGGCAAACA TACCGGGAGC CATACCGCAC TGGCAGCTTC TCTACAGGGC TCGAGAAACC
    TATGGGCTGC CCAACACACT GCCTACCGCC TGGCACAACC TGGTATATCG CATGCGGGGC
    GACATGCAAC TTTTCCAGAC CTTCTGGTTT CTCTACCATA AGGGCCACCC ACCCTCGGAG
    CCCTGTGGCA CGCCCTGCCG TCTGGCTACT CTTTGTGCCC AGCTCTCTGC CCGTGCTGAC
    AGCCCTGCTC TGTGCCGCCA CCTGATGCCA GATGGGAGCC TCCCAGAGGC CCAGAGCCTG
    TGGCCAAGGC CACTGTTTTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352064.1
    CDS126..1889
    Translation

    Target ORF information:

    RefSeq Version NM_001365135.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365135.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
    ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
    GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
    TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
    CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGGTTACC GTGTGTACCA AATAGATGGA
    AACTACTCCG GGAGCTCTCA CGTGGTCCTG GACCATGAGA CCTACATCCT GAATCTGACC
    CAGGCAAACA TACCGGGAGC CATACCGCAC TGGCAGCTTC TCTACAGGGC TCGAGAAACC
    TATGGGCTGC CCAACACACT GCCTACCGCC TGGCACAACC TGGTATATCG CATGCGGGGC
    GACATGCAAC TTTTCCAGAC CTTCTGGTTT CTCTACCATA AGGGCCACCC ACCCTCGGAG
    CCCTGTGGCA CGCCCTGCCG TCTGGCTACT CTTTGTGCCC AGCTCTCTGC CCGTGCTGAC
    AGCCCTGCTC TGTGCCGCCA CCTGATGCCA GATGGGAGCC TCCCAGAGGC CCAGAGCCTG
    TGGCCAAGGC CACTGTTTTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60647
    Clone ID Related Accession (Same CDS sequence) NM_001365135.1 , NM_001365135.2 , XM_011520303.1
    Accession Version NM_001365135.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068733.12. On May 31, 2019 this sequence version replaced NM_001365135.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.665950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
    ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
    GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
    TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
    CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGGTTACC GTGTGTACCA AATAGATGGA
    AACTACTCCG GGAGCTCTCA CGTGGTCCTG GACCATGAGA CCTACATCCT GAATCTGACC
    CAGGCAAACA TACCGGGAGC CATACCGCAC TGGCAGCTTC TCTACAGGGC TCGAGAAACC
    TATGGGCTGC CCAACACACT GCCTACCGCC TGGCACAACC TGGTATATCG CATGCGGGGC
    GACATGCAAC TTTTCCAGAC CTTCTGGTTT CTCTACCATA AGGGCCACCC ACCCTCGGAG
    CCCTGTGGCA CGCCCTGCCG TCTGGCTACT CTTTGTGCCC AGCTCTCTGC CCGTGCTGAC
    AGCCCTGCTC TGTGCCGCCA CCTGATGCCA GATGGGAGCC TCCCAGAGGC CCAGAGCCTG
    TGGCCAAGGC CACTGTTTTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352064.1
    CDS126..1889
    Translation

    Target ORF information:

    RefSeq Version NM_001365135.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365135.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
    ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
    GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
    TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
    CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGGTTACC GTGTGTACCA AATAGATGGA
    AACTACTCCG GGAGCTCTCA CGTGGTCCTG GACCATGAGA CCTACATCCT GAATCTGACC
    CAGGCAAACA TACCGGGAGC CATACCGCAC TGGCAGCTTC TCTACAGGGC TCGAGAAACC
    TATGGGCTGC CCAACACACT GCCTACCGCC TGGCACAACC TGGTATATCG CATGCGGGGC
    GACATGCAAC TTTTCCAGAC CTTCTGGTTT CTCTACCATA AGGGCCACCC ACCCTCGGAG
    CCCTGTGGCA CGCCCTGCCG TCTGGCTACT CTTTGTGCCC AGCTCTCTGC CCGTGCTGAC
    AGCCCTGCTC TGTGCCGCCA CCTGATGCCA GATGGGAGCC TCCCAGAGGC CCAGAGCCTG
    TGGCCAAGGC CACTGTTTTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60647
    Clone ID Related Accession (Same CDS sequence) NM_001365135.1 , NM_001365135.2 , XM_011520303.1
    Accession Version XM_011520303.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
    ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
    GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
    TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
    CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGGTTACC GTGTGTACCA AATAGATGGA
    AACTACTCCG GGAGCTCTCA CGTGGTCCTG GACCATGAGA CCTACATCCT GAATCTGACC
    CAGGCAAACA TACCGGGAGC CATACCGCAC TGGCAGCTTC TCTACAGGGC TCGAGAAACC
    TATGGGCTGC CCAACACACT GCCTACCGCC TGGCACAACC TGGTATATCG CATGCGGGGC
    GACATGCAAC TTTTCCAGAC CTTCTGGTTT CTCTACCATA AGGGCCACCC ACCCTCGGAG
    CCCTGTGGCA CGCCCTGCCG TCTGGCTACT CTTTGTGCCC AGCTCTCTGC CCGTGCTGAC
    AGCCCTGCTC TGTGCCGCCA CCTGATGCCA GATGGGAGCC TCCCAGAGGC CCAGAGCCTG
    TGGCCAAGGC CACTGTTTTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011518605.1
    CDS299..2062
    Translation

    Target ORF information:

    RefSeq Version XM_011520303.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011520303.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
    ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
    GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
    TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
    CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGGTTACC GTGTGTACCA AATAGATGGA
    AACTACTCCG GGAGCTCTCA CGTGGTCCTG GACCATGAGA CCTACATCCT GAATCTGACC
    CAGGCAAACA TACCGGGAGC CATACCGCAC TGGCAGCTTC TCTACAGGGC TCGAGAAACC
    TATGGGCTGC CCAACACACT GCCTACCGCC TGGCACAACC TGGTATATCG CATGCGGGGC
    GACATGCAAC TTTTCCAGAC CTTCTGGTTT CTCTACCATA AGGGCCACCC ACCCTCGGAG
    CCCTGTGGCA CGCCCTGCCG TCTGGCTACT CTTTGTGCCC AGCTCTCTGC CCGTGCTGAC
    AGCCCTGCTC TGTGCCGCCA CCTGATGCCA GATGGGAGCC TCCCAGAGGC CCAGAGCCTG
    TGGCCAAGGC CACTGTTTTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60648
    Clone ID Related Accession (Same CDS sequence) XM_011520304.1 , XM_011520304.2
    Accession Version XM_011520304.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
    ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
    GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
    TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
    CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGTCAGCC CCACATCCTT GCAGGTTACC
    GTGTGTACCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011518606.1
    CDS299..1693
    Translation

    Target ORF information:

    RefSeq Version XM_011520304.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011520304.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
    ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
    GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
    TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
    CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGTCAGCC CCACATCCTT GCAGGTTACC
    GTGTGTACCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60648
    Clone ID Related Accession (Same CDS sequence) XM_011520304.1 , XM_011520304.2
    Accession Version XM_011520304.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011520304.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
    ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
    GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
    TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
    CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGTCAGCC CCACATCCTT GCAGGTTACC
    GTGTGTACCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011518606.1
    CDS126..1520
    Translation

    Target ORF information:

    RefSeq Version XM_011520304.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011520304.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
    ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
    GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
    TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
    CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGTCAGCC CCACATCCTT GCAGGTTACC
    GTGTGTACCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101203
    Clone ID Related Accession (Same CDS sequence) NM_001318088.1 , NM_001318088.2
    Accession Version NM_001318088.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, HQ132748.1, M59916.1, AB209775.1 and BM977655.1. On Dec 23, 2015 this sequence version replaced XR_930886.1. Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate translation start site compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus, and lacks a predicted signal peptide compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HQ132748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAGCAC ACCTGTCAAT AGCTTCCCTC CCCCCTTCAT TGAGGGCAAC CACTCCTCCC 
    GCTGGCTCTA TGAAGCGATG GCCAAGGCTT GGGAGCCCTG GCTGCCTGCC GAAGCCCTGC
    GCACCCTCAG GTACTTATCG TCCGTGGAAA CCCAGGAAGG GAAAAGAAAG AATTGGGGGG
    TTCTATGCTC TTTCCCCATA CCCCGGTCTC CGCCTCATCT CTCTCAATAT GAATTTTTGT
    TCCCGTGAGA ACTTCTGGCT CTTGATCAAC TCCACGGATC CCGCAGGACA GCTCCAGTGG
    CTGGTGGGGG AGCTTCAGGC TGCTGAGGAT CGAGGAGACA AAGTGCATAT AATTGGCCAC
    ATTCCCCCAG GGCACTGTCT GAAGAGCTGG AGCTGGAATT ATTACCGAAT TGTAGCCAGG
    TATGAGAACA CCCTGGCTGC TCAGTTCTTT GGCCACACTC ATGTGGATGA ATTTGAGGTC
    TTCTATGATG AAGAGACTCT GAGCCGGCCG CTGGCTGTAG CCTTCCTGGC ACCCAGTGCA
    ACTACCTACA TCGGCCTTAA TCCTGGTTAC CGTGTGTACC AAATAGATGG AAACTACTCC
    GGGAGCTCTC ACGTGGTCCT GGACCATGAG ACCTACATCC TGAATCTGAC CCAGGCAAAC
    ATACCGGGAG CCATACCGCA CTGGCAGCTT CTCTACAGGG CTCGAGAAAC CTATGGGCTG
    CCCAACACAC TGCCTACCGC CTGGCACAAC CTGGTATATC GCATGCGGGG CGACATGCAA
    CTTTTCCAGA CCTTCTGGTT TCTCTACCAT AAGGGCCACC CACCCTCGGA GCCCTGTGGC
    ACGCCCTGCC GTCTGGCTAC TCTTTGTGCC CAGCTCTCTG CCCGTGCTGA CAGCCCTGCT
    CTGTGCCGCC ACCTGATGCC AGATGGGAGC CTCCCAGAGG CCCAGAGCCT GTGGCCAAGG
    CCACTGTTTT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001305017.1
    CDS1147..2121
    Translation

    Target ORF information:

    RefSeq Version NM_001318088.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001318088.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAGCAC ACCTGTCAAT AGCTTCCCTC CCCCCTTCAT TGAGGGCAAC CACTCCTCCC 
    GCTGGCTCTA TGAAGCGATG GCCAAGGCTT GGGAGCCCTG GCTGCCTGCC GAAGCCCTGC
    GCACCCTCAG GTACTTATCG TCCGTGGAAA CCCAGGAAGG GAAAAGAAAG AATTGGGGGG
    TTCTATGCTC TTTCCCCATA CCCCGGTCTC CGCCTCATCT CTCTCAATAT GAATTTTTGT
    TCCCGTGAGA ACTTCTGGCT CTTGATCAAC TCCACGGATC CCGCAGGACA GCTCCAGTGG
    CTGGTGGGGG AGCTTCAGGC TGCTGAGGAT CGAGGAGACA AAGTGCATAT AATTGGCCAC
    ATTCCCCCAG GGCACTGTCT GAAGAGCTGG AGCTGGAATT ATTACCGAAT TGTAGCCAGG
    TATGAGAACA CCCTGGCTGC TCAGTTCTTT GGCCACACTC ATGTGGATGA ATTTGAGGTC
    TTCTATGATG AAGAGACTCT GAGCCGGCCG CTGGCTGTAG CCTTCCTGGC ACCCAGTGCA
    ACTACCTACA TCGGCCTTAA TCCTGGTTAC CGTGTGTACC AAATAGATGG AAACTACTCC
    GGGAGCTCTC ACGTGGTCCT GGACCATGAG ACCTACATCC TGAATCTGAC CCAGGCAAAC
    ATACCGGGAG CCATACCGCA CTGGCAGCTT CTCTACAGGG CTCGAGAAAC CTATGGGCTG
    CCCAACACAC TGCCTACCGC CTGGCACAAC CTGGTATATC GCATGCGGGG CGACATGCAA
    CTTTTCCAGA CCTTCTGGTT TCTCTACCAT AAGGGCCACC CACCCTCGGA GCCCTGTGGC
    ACGCCCTGCC GTCTGGCTAC TCTTTGTGCC CAGCTCTCTG CCCGTGCTGA CAGCCCTGCT
    CTGTGCCGCC ACCTGATGCC AGATGGGAGC CTCCCAGAGG CCCAGAGCCT GTGGCCAAGG
    CCACTGTTTT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101203
    Clone ID Related Accession (Same CDS sequence) NM_001318088.1 , NM_001318088.2
    Accession Version NM_001318088.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, HQ132748.1, M59916.1, AB209775.1 and BM977655.1. On May 31, 2019 this sequence version replaced NM_001318088.1. Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate translation start site compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus, and lacks a predicted signal peptide compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.196116.1, HQ132748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAGCAC ACCTGTCAAT AGCTTCCCTC CCCCCTTCAT TGAGGGCAAC CACTCCTCCC 
    GCTGGCTCTA TGAAGCGATG GCCAAGGCTT GGGAGCCCTG GCTGCCTGCC GAAGCCCTGC
    GCACCCTCAG GTACTTATCG TCCGTGGAAA CCCAGGAAGG GAAAAGAAAG AATTGGGGGG
    TTCTATGCTC TTTCCCCATA CCCCGGTCTC CGCCTCATCT CTCTCAATAT GAATTTTTGT
    TCCCGTGAGA ACTTCTGGCT CTTGATCAAC TCCACGGATC CCGCAGGACA GCTCCAGTGG
    CTGGTGGGGG AGCTTCAGGC TGCTGAGGAT CGAGGAGACA AAGTGCATAT AATTGGCCAC
    ATTCCCCCAG GGCACTGTCT GAAGAGCTGG AGCTGGAATT ATTACCGAAT TGTAGCCAGG
    TATGAGAACA CCCTGGCTGC TCAGTTCTTT GGCCACACTC ATGTGGATGA ATTTGAGGTC
    TTCTATGATG AAGAGACTCT GAGCCGGCCG CTGGCTGTAG CCTTCCTGGC ACCCAGTGCA
    ACTACCTACA TCGGCCTTAA TCCTGGTTAC CGTGTGTACC AAATAGATGG AAACTACTCC
    GGGAGCTCTC ACGTGGTCCT GGACCATGAG ACCTACATCC TGAATCTGAC CCAGGCAAAC
    ATACCGGGAG CCATACCGCA CTGGCAGCTT CTCTACAGGG CTCGAGAAAC CTATGGGCTG
    CCCAACACAC TGCCTACCGC CTGGCACAAC CTGGTATATC GCATGCGGGG CGACATGCAA
    CTTTTCCAGA CCTTCTGGTT TCTCTACCAT AAGGGCCACC CACCCTCGGA GCCCTGTGGC
    ACGCCCTGCC GTCTGGCTAC TCTTTGTGCC CAGCTCTCTG CCCGTGCTGA CAGCCCTGCT
    CTGTGCCGCC ACCTGATGCC AGATGGGAGC CTCCCAGAGG CCCAGAGCCT GTGGCCAAGG
    CCACTGTTTT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001305017.1
    CDS1087..2061
    Translation

    Target ORF information:

    RefSeq Version NM_001318088.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001318088.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAGCAC ACCTGTCAAT AGCTTCCCTC CCCCCTTCAT TGAGGGCAAC CACTCCTCCC 
    GCTGGCTCTA TGAAGCGATG GCCAAGGCTT GGGAGCCCTG GCTGCCTGCC GAAGCCCTGC
    GCACCCTCAG GTACTTATCG TCCGTGGAAA CCCAGGAAGG GAAAAGAAAG AATTGGGGGG
    TTCTATGCTC TTTCCCCATA CCCCGGTCTC CGCCTCATCT CTCTCAATAT GAATTTTTGT
    TCCCGTGAGA ACTTCTGGCT CTTGATCAAC TCCACGGATC CCGCAGGACA GCTCCAGTGG
    CTGGTGGGGG AGCTTCAGGC TGCTGAGGAT CGAGGAGACA AAGTGCATAT AATTGGCCAC
    ATTCCCCCAG GGCACTGTCT GAAGAGCTGG AGCTGGAATT ATTACCGAAT TGTAGCCAGG
    TATGAGAACA CCCTGGCTGC TCAGTTCTTT GGCCACACTC ATGTGGATGA ATTTGAGGTC
    TTCTATGATG AAGAGACTCT GAGCCGGCCG CTGGCTGTAG CCTTCCTGGC ACCCAGTGCA
    ACTACCTACA TCGGCCTTAA TCCTGGTTAC CGTGTGTACC AAATAGATGG AAACTACTCC
    GGGAGCTCTC ACGTGGTCCT GGACCATGAG ACCTACATCC TGAATCTGAC CCAGGCAAAC
    ATACCGGGAG CCATACCGCA CTGGCAGCTT CTCTACAGGG CTCGAGAAAC CTATGGGCTG
    CCCAACACAC TGCCTACCGC CTGGCACAAC CTGGTATATC GCATGCGGGG CGACATGCAA
    CTTTTCCAGA CCTTCTGGTT TCTCTACCAT AAGGGCCACC CACCCTCGGA GCCCTGTGGC
    ACGCCCTGCC GTCTGGCTAC TCTTTGTGCC CAGCTCTCTG CCCGTGCTGA CAGCCCTGCT
    CTGTGCCGCC ACCTGATGCC AGATGGGAGC CTCCCAGAGG CCCAGAGCCT GTGGCCAAGG
    CCACTGTTTT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16724
    Clone ID Related Accession (Same CDS sequence) NM_001007593.2 , NM_001007593.3
    Accession Version NM_001007593.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, AB209775.1, M59916.1 and AA746054.1. On Jul 17, 2010 this sequence version replaced NM_001007593.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded protein (isoform 2) is one amino acid shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292388.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGGA ACCCAATGTG GCTCGCGTGG GCTCCGTGGC CATCAAGCTG
    TGCAATCTGC TGAAGATAGC ACCACCTGCC GTGTGCCAAT CCATTGTCCA CCTCTTTGAG
    GATGACATGG TGGAGGTGTG GAGACGCTCA GTGCTGAGCC CATCTGAGGC CTGTGGCCTG
    CTCCTGGGCT CCACCTGTGG GCACTGGGAC ATTTTCTCAT CTTGGAACAT CTCTTTGCCT
    ACTGTGCCGA AGCCGCCCCC CAAACCCCCT AGCCCCCCAG CCCCAGGTGC CCCTGTCAGC
    CGCATCCTCT TCCTCACTGA CCTGCACTGG GATCATGACT ACCTGGAGGG CACGGACCCT
    GACTGTGCAG ACCCACTGTG CTGCCGCCGG GGTTCTGGCC TGCCGCCCGC ATCCCGGCCA
    GGTGCCGGAT ACTGGGGCGA ATACAGCAAG TGTGACCTGC CCCTGAGGAC CCTGGAGAGC
    CTGTTGAGTG GGCTGGGCCC AGCCGGCCCT TTTGATATGG TGTACTGGAC AGGAGACATC
    CCCGCACATG ATGTCTGGCA CCAGACTCGT CAGGACCAAC TGCGGGCCCT GACCACCGTC
    ACAGCACTTG TGAGGAAGTT CCTGGGGCCA GTGCCAGTGT ACCCTGCTGT GGGTAACCAT
    GAAAGCACAC CTGTCAATAG CTTCCCTCCC CCCTTCATTG AGGGCAACCA CTCCTCCCGC
    TGGCTCTATG AAGCGATGGC CAAGGCTTGG GAGCCCTGGC TGCCTGCCGA AGCCCTGCGC
    ACCCTCAGAA TTGGGGGGTT CTATGCTCTT TCCCCATACC CCGGTCTCCG CCTCATCTCT
    CTCAATATGA ATTTTTGTTC CCGTGAGAAC TTCTGGCTCT TGATCAACTC CACGGATCCC
    GCAGGACAGC TCCAGTGGCT GGTGGGGGAG CTTCAGGCTG CTGAGGATCG AGGAGACAAA
    GTGCATATAA TTGGCCACAT TCCCCCAGGG CACTGTCTGA AGAGCTGGAG CTGGAATTAT
    TACCGAATTG TAGCCAGGTA TGAGAACACC CTGGCTGCTC AGTTCTTTGG CCACACTCAT
    GTGGATGAAT TTGAGGTCTT CTATGATGAA GAGACTCTGA GCCGGCCGCT GGCTGTAGCC
    TTCCTGGCAC CCAGTGCAAC TACCTACATC GGCCTTAATC CTGGTTACCG TGTGTACCAA
    ATAGATGGAA ACTACTCCGG GAGCTCTCAC GTGGTCCTGG ACCATGAGAC CTACATCCTG
    AATCTGACCC AGGCAAACAT ACCGGGAGCC ATACCGCACT GGCAGCTTCT CTACAGGGCT
    CGAGAAACCT ATGGGCTGCC CAACACACTG CCTACCGCCT GGCACAACCT GGTATATCGC
    ATGCGGGGCG ACATGCAACT TTTCCAGACC TTCTGGTTTC TCTACCATAA GGGCCACCCA
    CCCTCGGAGC CCTGTGGCAC GCCCTGCCGT CTGGCTACTC TTTGTGCCCA GCTCTCTGCC
    CGTGCTGACA GCCCTGCTCT GTGCCGCCAC CTGATGCCAG ATGGGAGCCT CCCAGAGGCC
    CAGAGCCTGT GGCCAAGGCC ACTGTTTTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001007594.2
    CDS186..2078
    Translation

    Target ORF information:

    RefSeq Version NM_001007593.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001007593.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGGA ACCCAATGTG GCTCGCGTGG GCTCCGTGGC CATCAAGCTG
    TGCAATCTGC TGAAGATAGC ACCACCTGCC GTGTGCCAAT CCATTGTCCA CCTCTTTGAG
    GATGACATGG TGGAGGTGTG GAGACGCTCA GTGCTGAGCC CATCTGAGGC CTGTGGCCTG
    CTCCTGGGCT CCACCTGTGG GCACTGGGAC ATTTTCTCAT CTTGGAACAT CTCTTTGCCT
    ACTGTGCCGA AGCCGCCCCC CAAACCCCCT AGCCCCCCAG CCCCAGGTGC CCCTGTCAGC
    CGCATCCTCT TCCTCACTGA CCTGCACTGG GATCATGACT ACCTGGAGGG CACGGACCCT
    GACTGTGCAG ACCCACTGTG CTGCCGCCGG GGTTCTGGCC TGCCGCCCGC ATCCCGGCCA
    GGTGCCGGAT ACTGGGGCGA ATACAGCAAG TGTGACCTGC CCCTGAGGAC CCTGGAGAGC
    CTGTTGAGTG GGCTGGGCCC AGCCGGCCCT TTTGATATGG TGTACTGGAC AGGAGACATC
    CCCGCACATG ATGTCTGGCA CCAGACTCGT CAGGACCAAC TGCGGGCCCT GACCACCGTC
    ACAGCACTTG TGAGGAAGTT CCTGGGGCCA GTGCCAGTGT ACCCTGCTGT GGGTAACCAT
    GAAAGCACAC CTGTCAATAG CTTCCCTCCC CCCTTCATTG AGGGCAACCA CTCCTCCCGC
    TGGCTCTATG AAGCGATGGC CAAGGCTTGG GAGCCCTGGC TGCCTGCCGA AGCCCTGCGC
    ACCCTCAGAA TTGGGGGGTT CTATGCTCTT TCCCCATACC CCGGTCTCCG CCTCATCTCT
    CTCAATATGA ATTTTTGTTC CCGTGAGAAC TTCTGGCTCT TGATCAACTC CACGGATCCC
    GCAGGACAGC TCCAGTGGCT GGTGGGGGAG CTTCAGGCTG CTGAGGATCG AGGAGACAAA
    GTGCATATAA TTGGCCACAT TCCCCCAGGG CACTGTCTGA AGAGCTGGAG CTGGAATTAT
    TACCGAATTG TAGCCAGGTA TGAGAACACC CTGGCTGCTC AGTTCTTTGG CCACACTCAT
    GTGGATGAAT TTGAGGTCTT CTATGATGAA GAGACTCTGA GCCGGCCGCT GGCTGTAGCC
    TTCCTGGCAC CCAGTGCAAC TACCTACATC GGCCTTAATC CTGGTTACCG TGTGTACCAA
    ATAGATGGAA ACTACTCCGG GAGCTCTCAC GTGGTCCTGG ACCATGAGAC CTACATCCTG
    AATCTGACCC AGGCAAACAT ACCGGGAGCC ATACCGCACT GGCAGCTTCT CTACAGGGCT
    CGAGAAACCT ATGGGCTGCC CAACACACTG CCTACCGCCT GGCACAACCT GGTATATCGC
    ATGCGGGGCG ACATGCAACT TTTCCAGACC TTCTGGTTTC TCTACCATAA GGGCCACCCA
    CCCTCGGAGC CCTGTGGCAC GCCCTGCCGT CTGGCTACTC TTTGTGCCCA GCTCTCTGCC
    CGTGCTGACA GCCCTGCTCT GTGCCGCCAC CTGATGCCAG ATGGGAGCCT CCCAGAGGCC
    CAGAGCCTGT GGCCAAGGCC ACTGTTTTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16724
    Clone ID Related Accession (Same CDS sequence) NM_001007593.2 , NM_001007593.3
    Accession Version NM_001007593.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB209775.1, M59916.1 and AA746054.1. On May 31, 2019 this sequence version replaced NM_001007593.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded protein (isoform 2) is one amino acid shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292388.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGGA ACCCAATGTG GCTCGCGTGG GCTCCGTGGC CATCAAGCTG
    TGCAATCTGC TGAAGATAGC ACCACCTGCC GTGTGCCAAT CCATTGTCCA CCTCTTTGAG
    GATGACATGG TGGAGGTGTG GAGACGCTCA GTGCTGAGCC CATCTGAGGC CTGTGGCCTG
    CTCCTGGGCT CCACCTGTGG GCACTGGGAC ATTTTCTCAT CTTGGAACAT CTCTTTGCCT
    ACTGTGCCGA AGCCGCCCCC CAAACCCCCT AGCCCCCCAG CCCCAGGTGC CCCTGTCAGC
    CGCATCCTCT TCCTCACTGA CCTGCACTGG GATCATGACT ACCTGGAGGG CACGGACCCT
    GACTGTGCAG ACCCACTGTG CTGCCGCCGG GGTTCTGGCC TGCCGCCCGC ATCCCGGCCA
    GGTGCCGGAT ACTGGGGCGA ATACAGCAAG TGTGACCTGC CCCTGAGGAC CCTGGAGAGC
    CTGTTGAGTG GGCTGGGCCC AGCCGGCCCT TTTGATATGG TGTACTGGAC AGGAGACATC
    CCCGCACATG ATGTCTGGCA CCAGACTCGT CAGGACCAAC TGCGGGCCCT GACCACCGTC
    ACAGCACTTG TGAGGAAGTT CCTGGGGCCA GTGCCAGTGT ACCCTGCTGT GGGTAACCAT
    GAAAGCACAC CTGTCAATAG CTTCCCTCCC CCCTTCATTG AGGGCAACCA CTCCTCCCGC
    TGGCTCTATG AAGCGATGGC CAAGGCTTGG GAGCCCTGGC TGCCTGCCGA AGCCCTGCGC
    ACCCTCAGAA TTGGGGGGTT CTATGCTCTT TCCCCATACC CCGGTCTCCG CCTCATCTCT
    CTCAATATGA ATTTTTGTTC CCGTGAGAAC TTCTGGCTCT TGATCAACTC CACGGATCCC
    GCAGGACAGC TCCAGTGGCT GGTGGGGGAG CTTCAGGCTG CTGAGGATCG AGGAGACAAA
    GTGCATATAA TTGGCCACAT TCCCCCAGGG CACTGTCTGA AGAGCTGGAG CTGGAATTAT
    TACCGAATTG TAGCCAGGTA TGAGAACACC CTGGCTGCTC AGTTCTTTGG CCACACTCAT
    GTGGATGAAT TTGAGGTCTT CTATGATGAA GAGACTCTGA GCCGGCCGCT GGCTGTAGCC
    TTCCTGGCAC CCAGTGCAAC TACCTACATC GGCCTTAATC CTGGTTACCG TGTGTACCAA
    ATAGATGGAA ACTACTCCGG GAGCTCTCAC GTGGTCCTGG ACCATGAGAC CTACATCCTG
    AATCTGACCC AGGCAAACAT ACCGGGAGCC ATACCGCACT GGCAGCTTCT CTACAGGGCT
    CGAGAAACCT ATGGGCTGCC CAACACACTG CCTACCGCCT GGCACAACCT GGTATATCGC
    ATGCGGGGCG ACATGCAACT TTTCCAGACC TTCTGGTTTC TCTACCATAA GGGCCACCCA
    CCCTCGGAGC CCTGTGGCAC GCCCTGCCGT CTGGCTACTC TTTGTGCCCA GCTCTCTGCC
    CGTGCTGACA GCCCTGCTCT GTGCCGCCAC CTGATGCCAG ATGGGAGCCT CCCAGAGGCC
    CAGAGCCTGT GGCCAAGGCC ACTGTTTTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001007594.2
    CDS126..2018
    Translation

    Target ORF information:

    RefSeq Version NM_001007593.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001007593.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGGA ACCCAATGTG GCTCGCGTGG GCTCCGTGGC CATCAAGCTG
    TGCAATCTGC TGAAGATAGC ACCACCTGCC GTGTGCCAAT CCATTGTCCA CCTCTTTGAG
    GATGACATGG TGGAGGTGTG GAGACGCTCA GTGCTGAGCC CATCTGAGGC CTGTGGCCTG
    CTCCTGGGCT CCACCTGTGG GCACTGGGAC ATTTTCTCAT CTTGGAACAT CTCTTTGCCT
    ACTGTGCCGA AGCCGCCCCC CAAACCCCCT AGCCCCCCAG CCCCAGGTGC CCCTGTCAGC
    CGCATCCTCT TCCTCACTGA CCTGCACTGG GATCATGACT ACCTGGAGGG CACGGACCCT
    GACTGTGCAG ACCCACTGTG CTGCCGCCGG GGTTCTGGCC TGCCGCCCGC ATCCCGGCCA
    GGTGCCGGAT ACTGGGGCGA ATACAGCAAG TGTGACCTGC CCCTGAGGAC CCTGGAGAGC
    CTGTTGAGTG GGCTGGGCCC AGCCGGCCCT TTTGATATGG TGTACTGGAC AGGAGACATC
    CCCGCACATG ATGTCTGGCA CCAGACTCGT CAGGACCAAC TGCGGGCCCT GACCACCGTC
    ACAGCACTTG TGAGGAAGTT CCTGGGGCCA GTGCCAGTGT ACCCTGCTGT GGGTAACCAT
    GAAAGCACAC CTGTCAATAG CTTCCCTCCC CCCTTCATTG AGGGCAACCA CTCCTCCCGC
    TGGCTCTATG AAGCGATGGC CAAGGCTTGG GAGCCCTGGC TGCCTGCCGA AGCCCTGCGC
    ACCCTCAGAA TTGGGGGGTT CTATGCTCTT TCCCCATACC CCGGTCTCCG CCTCATCTCT
    CTCAATATGA ATTTTTGTTC CCGTGAGAAC TTCTGGCTCT TGATCAACTC CACGGATCCC
    GCAGGACAGC TCCAGTGGCT GGTGGGGGAG CTTCAGGCTG CTGAGGATCG AGGAGACAAA
    GTGCATATAA TTGGCCACAT TCCCCCAGGG CACTGTCTGA AGAGCTGGAG CTGGAATTAT
    TACCGAATTG TAGCCAGGTA TGAGAACACC CTGGCTGCTC AGTTCTTTGG CCACACTCAT
    GTGGATGAAT TTGAGGTCTT CTATGATGAA GAGACTCTGA GCCGGCCGCT GGCTGTAGCC
    TTCCTGGCAC CCAGTGCAAC TACCTACATC GGCCTTAATC CTGGTTACCG TGTGTACCAA
    ATAGATGGAA ACTACTCCGG GAGCTCTCAC GTGGTCCTGG ACCATGAGAC CTACATCCTG
    AATCTGACCC AGGCAAACAT ACCGGGAGCC ATACCGCACT GGCAGCTTCT CTACAGGGCT
    CGAGAAACCT ATGGGCTGCC CAACACACTG CCTACCGCCT GGCACAACCT GGTATATCGC
    ATGCGGGGCG ACATGCAACT TTTCCAGACC TTCTGGTTTC TCTACCATAA GGGCCACCCA
    CCCTCGGAGC CCTGTGGCAC GCCCTGCCGT CTGGCTACTC TTTGTGCCCA GCTCTCTGCC
    CGTGCTGACA GCCCTGCTCT GTGCCGCCAC CTGATGCCAG ATGGGAGCCT CCCAGAGGCC
    CAGAGCCTGT GGCCAAGGCC ACTGTTTTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44952
    Clone ID Related Accession (Same CDS sequence) NM_001318087.1 , NM_001318087.2
    Accession Version NM_001318087.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, HQ132747.1, M59916.1, BC041164.1 and AA746054.1. On Dec 23, 2015 this sequence version replaced XM_005253075.3. Transcript Variant: This variant (3) uses an alternate splice site in its 3' exon resulting in a frameshift and an early stop codon compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HQ132747.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
    TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
    CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
    AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
    TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
    CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
    GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGTCAG CCCCACATCC
    TTGCAGGTTA CCGTGTGTAC CAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001305016.1
    CDS186..1712
    Translation

    Target ORF information:

    RefSeq Version NM_001318087.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001318087.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
    TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
    CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
    AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
    TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
    CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
    GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGTCAG CCCCACATCC
    TTGCAGGTTA CCGTGTGTAC CAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44952
    Clone ID Related Accession (Same CDS sequence) NM_001318087.1 , NM_001318087.2
    Accession Version NM_001318087.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, HQ132747.1, M59916.1 and BC041164.1. On May 31, 2019 this sequence version replaced NM_001318087.1. Transcript Variant: This variant (3) uses an alternate splice site in its 3' exon resulting in a frameshift and an early stop codon compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.975980.1, SRR3476690.310773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
    TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
    CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
    AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
    TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
    CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
    GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGTCAG CCCCACATCC
    TTGCAGGTTA CCGTGTGTAC CAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001305016.1
    CDS126..1652
    Translation

    Target ORF information:

    RefSeq Version NM_001318087.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001318087.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
    CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
    CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
    GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
    CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
    ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
    CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
    GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
    CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
    CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
    AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
    CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
    CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
    AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
    ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
    GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
    CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
    CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
    CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
    TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
    CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
    AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
    TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
    CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
    GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGTCAG CCCCACATCC
    TTGCAGGTTA CCGTGTGTAC CAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.