WNK2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	WNK2
Entrez Gene ID	65268
Full Name	WNK lysine deficient protein kinase 2
Synonyms	NY-CO-43,P/OKcl.13,PRKWNK2,SDCCAG43
General protein information	Preferred Names WNK lysine deficient protein kinase 2 Names serine/threonine-protein kinase WNK2 antigen NY-CO-43 mitogen-activated protein kinase kinase kinase protein kinase lysine-deficient 2 protein kinase with no lysine 2 serologically defined colon cancer antigen 43
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q22.31
Summary	The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling survival, and proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013].
Disorder MIM:	606249

mRNA and Protein(s)

mRNA	Protein	Name
NM_001282394.1	NP_001269323.1	serine/threonine-protein kinase WNK2 isoform 1
XM_011518928.2	XP_011517230.1	serine/threonine-protein kinase WNK2 isoform X3
XM_011518932.2	XP_011517234.1	serine/threonine-protein kinase WNK2 isoform X10
XM_017015048.1	XP_016870537.1	serine/threonine-protein kinase WNK2 isoform X12
XM_005252144.2	XP_005252201.1	serine/threonine-protein kinase WNK2 isoform X22
XM_011518940.2	XP_011517242.1	serine/threonine-protein kinase WNK2 isoform X26
XM_011518926.2	XP_011517228.1	serine/threonine-protein kinase WNK2 isoform X1
XM_011518929.2	XP_011517231.1	serine/threonine-protein kinase WNK2 isoform X4
XM_017015046.1	XP_016870535.1	serine/threonine-protein kinase WNK2 isoform X8
XM_011518931.2	XP_011517233.1	serine/threonine-protein kinase WNK2 isoform X9
XM_017015045.1	XP_016870534.1	serine/threonine-protein kinase WNK2 isoform X6
XM_017015049.1	XP_016870538.1	serine/threonine-protein kinase WNK2 isoform X13
XM_017015050.1	XP_016870539.1	serine/threonine-protein kinase WNK2 isoform X14
XM_005252140.2	XP_005252197.1	serine/threonine-protein kinase WNK2 isoform X15
XM_017015051.1	XP_016870540.1	serine/threonine-protein kinase WNK2 isoform X17
XM_017015052.1	XP_016870541.1	serine/threonine-protein kinase WNK2 isoform X18
XM_017015053.1	XP_016870542.1	serine/threonine-protein kinase WNK2 isoform X19
XM_011518937.2	XP_011517239.1	serine/threonine-protein kinase WNK2 isoform X25
NM_006648.3	NP_006639.3	serine/threonine-protein kinase WNK2 isoform 2
XM_005252137.3	XP_005252194.1	serine/threonine-protein kinase WNK2 isoform X5
XM_017015047.1	XP_016870536.1	serine/threonine-protein kinase WNK2 isoform X11
XM_005252141.2	XP_005252198.1	serine/threonine-protein kinase WNK2 isoform X16
XM_011518935.2	XP_011517237.1	serine/threonine-protein kinase WNK2 isoform X20
XM_011518936.2	XP_011517238.1	serine/threonine-protein kinase WNK2 isoform X21
XM_017015055.1	XP_016870544.1	serine/threonine-protein kinase WNK2 isoform X24
XM_011518939.2	XP_011517241.1	serine/threonine-protein kinase WNK2 isoform X26
XM_011518927.2	XP_011517229.1	serine/threonine-protein kinase WNK2 isoform X2
XM_011518930.2	XP_011517232.1	serine/threonine-protein kinase WNK2 isoform X7
XM_017015054.1	XP_016870543.1	serine/threonine-protein kinase WNK2 isoform X23
XM_011518937.3	XP_011517239.1	serine/threonine-protein kinase WNK2 isoform X25

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	WNK2	XP_003951524.1
Canis lupus familiaris (dog)	WNK2	XP_005616885.1
Xenopus tropicalis (tropical clawed frog)	wnk2	XP_002943109.2
Homo sapiens (human)	WNK2	NP_006639.3
	WNK2	XP_001108626.2
Bos taurus (cattle)	WNK2	XP_005210503.1
Mus musculus (house mouse)	Wnk2	NP_083637.2
Rattus norvegicus (Norway rat)	Wnk2	NP_001178485.1
Gallus gallus (chicken)	WNK2	XP_414326.4

Gene Ontology
Bibliography

Related articles in PubMed

Silencing of the tumor suppressor gene WNK2 is associated with upregulation of MMP2 and JNK in gliomas.
Costa AM, Pinto F, Martinho O, Oliveira MJ, Jordan P, Reis RM
Oncotarget6(3)1422-34(2015 Jan)

Early epigenetic downregulation of WNK2 kinase during pancreatic ductal adenocarcinoma development.
Dutruel C, Bergmann F, Rooman I, Zucknick M, Weichenhan D, Geiselhart L, Kaffenberger T, Rachakonda PS, Bauer A, Giese N, Hong C, Xie H, Costello JF, Hoheisel J, Kumar R, Rehli M, Schirmacher P, Werner J, Plass C, Popanda O, Schmezer P
Oncogene33(26)3401-10(2014 Jun)

Wnk kinases are positive regulators of canonical Wnt/?-catenin signalling.
Serysheva E, Berhane H, Grumolato L, Demir K, Balmer S, Bodak M, Boutros M, Aaronson S, Mlodzik M, Jenny A
EMBO reports14(8)718-25(2013 Aug)

Loss of WNK2 expression by promoter gene methylation occurs in adult gliomas and triggers Rac1-mediated tumour cell invasiveness.
Moniz S, Martinho O, Pinto F, Sousa B, Loureiro C, Oliveira MJ, Moita LF, Honavar M, Pinheiro C, Pires M, Lopes JM, Jones C, Costello JF, Paredes J, Reis RM, Jordan P
Human molecular genetics22(1)84-95(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WNK2 promoter methylation and silencing in gliomas is associated with increased JNK activation and MMP2 expression and activity, thus explaining in part tumor cell invasion potential.
Title: Silencing of the tumor suppressor gene WNK2 is associated with upregulation of MMP2 and JNK in gliomas.

Downregulation of WNK2 by promoter hypermethylation occurs early in Pancreatic ductal adenocarcinoma pathogenesis and may support tumor cell growth via the ERK-MAPK pathway.
Title: Early epigenetic downregulation of WNK2 kinase during pancreatic ductal adenocarcinoma development.

Wnk kinases are positive regulators of canonical Wnt/beta-catenin signaling.
Title: Wnk kinases are positive regulators of canonical Wnt/?-catenin signalling.

results validate the WNK2 gene as a recurrent target for epigenetic silencing in glia-derived brain tumours and provide first mechanistic evidence for a tumour-suppressing role of WNK2 that is related to Rac1 signalling and tumour cell invasion and growth
Title: Loss of WNK2 expression by promoter gene methylation occurs in adult gliomas and triggers Rac1-mediated tumour cell invasiveness.

a role for WNK2 in the regulation of CCCs in the mammalian brain, with implications for both cell volume regulation and/or GABAergic signaling.
Title: WNK2 kinase is a novel regulator of essential neuronal cation-chloride cotransporters.

The following WNK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00017	NM_006648.3 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu00234	NM_001282394.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu00017	XM_005252141.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu43440	XM_005252137.3 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu43444	XM_005252140.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu43448	XM_005252144.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71647	XM_011518926.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71648	XM_011518927.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71649	XM_011518928.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71650	XM_011518929.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71651	XM_011518930.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71652	XM_011518931.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71653	XM_011518932.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71656	XM_011518935.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71657	XM_011518936.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71658	XM_011518937.3 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71660	XM_011518939.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu71660	XM_011518940.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu98844	XM_017015045.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu98845	XM_017015046.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu98846	XM_017015047.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu98847	XM_017015048.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu98848	XM_017015049.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu98849	XM_017015050.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu98850	XM_017015051.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu98851	XM_017015052.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu98852	XM_017015053.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu98853	XM_017015054.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu98854	XM_017015055.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
View Old Accession Versions >>
OHu71658	XM_011518937.2	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WNK2 ( NM_006648.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006639.3 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu00017
Clone ID Related Accession (Same CDS sequence)	NM_006648.3 , XM_005252141.2
Accession Version	NM_006648.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL390760.25, AL354991.11 and AL583839.12. On Jul 7, 2003 this sequence version replaced NM_006648.2. Transcript Variant: This variant (2) lacks an alternate exon in the coding region and uses an alternate splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTATGA CCGCACCTCG AGCAGGAGTG 
GGGATGCCAC GTCTGCCCCC AGCGCCCGGC CCTCTGTCCA CCACGGTCAT TCCCGGAGCC 
GCCCCGACCC TGTCCGTGCC CACACCAGAT CCTGAGAGTG AGAAGCCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006639.3
CDS	1..6654
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDPESEKPD

Target ORF information:

RefSeq Version	NM_006648.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006648.3

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTATGA CCGCACCTCG AGCAGGAGTG 
GGGATGCCAC GTCTGCCCCC AGCGCCCGGC CCTCTGTCCA CCACGGTCAT TCCCGGAGCC 
GCCCCGACCC TGTCCGTGCC CACACCAGAT CCTGAGAGTG AGAAGCCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( NM_001282394.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269323.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant 1, mRNA.

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CloneID	OHu00234
Clone ID Related Accession (Same CDS sequence)	NM_001282394.1
Accession Version	NM_001282394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ242724.4, HY151846.1 and AL583839.12. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ242724.4 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGGTCCATG CAGGGGGCTC ACCCTGCCCT GTCTCCCATG GCGCAGAGCA 
GCCTGTGGGG CTGTGTTCCT ATCCTTGTTT TCTGCTGAAT CAGCTCAGTC AAAGCAGCCT 
CCAGATTCAG CGCCCTATAA AGACCAGCTG TCCTCGAAGG AACAACCCAG CTTTCTAGCC 
AGTCAGCAGC TCCTGAGCCA GGCGGGCCCC AGCAACCCTC CTGGGGCACC CCCAGCCCCT 
TTGGCCCCCT CCTCCCCTCC TGTGACTGCT CTGCCCCAAG ATGGAGCAGC TCCAGCCACC 
AGCACCATGC CAGAGCCAGC GTCAGGAACT GCCAGCCAGG CAGGGGGTCC AGGGACACCT 
CAGGGGCTGA CCAGTGAGCT CGAGACGTCT CAGCCACTAG CGGAGACTCA CGAGGCCCCG 
CTTGCTGTGC AGCCCCTCGT GGTGGGCCTA GCACCTTGCA CTCCAGCTCC AGAGGCTGCC 
TCAACCAGGG ACGCCAGTGC CCCAAGGGAG CCCCTGCCAC CTCCTGCACC TGAGCCCAGC 
CCCCACAGCG GGACCCCACA GCCCGCCTTG GGTCAACCTG CTCCCCTGCT TCCTGCCGCA 
GTGGGGGCCG TCAGCCTGGC CACCTCCCAG CTCCCAAGCC CACCCCTGGG GCCCACCGTC 
CCCCCACAGC CACCCTCGGC CCTGGAGTCG GATGGGGAAG GGCCGCCCCC CAGGGTGGGC 
TTTGTGGACA GCACCATCAA GAGCCTGGAC GAGAAGCTGC GGACTCTGCT CTACCAGGAG 
CACGTGCCCA CCTCCTCAGC CTCAGCTGGG ACCCCTGTGG AGGTGGGCGA CAGAGACTTC 
ACCCTGGAGC CCCTGAGAGG GGACCAGCCC CGCTCAGAGG TCTGCGGGGG GGACCTGGCC 
CTGCCCCCAG TGCCTAAGGA GGCGGTCTCA GGGCGTGTCC AGCTGCCCCA GCCCTTGGTG 
GAGAAGTCAG AACTGGCCCC CACTCGAGGG GCCGTGATGG AGCAGGGCAC GTCCTCGTCA 
ATGACAGCAG AGTCGTCTCC CAGGAGTATG CTAGGCTATG ACAGAGATGG AAGGCAGGTG 
GCCTCAGACT CCCATGTGGT CCCCAGCGTC CCCCAGGATG TACCTGCTTT TGTGAGACCT 
GCACGTGTGG AGCCCACAGA CAGGGATGGT GGAGAAGCTG GAGAAAGCTC GGCAGAGCCC 
CCGCCGAGTG ACATGGGCAC AGTGGGGGGC CAGGCTAGCC ACCCCCAGAC ACTCGGCGCT 
CGAGCTTTGG GGTCCCCTCG GAAACGTCCA GAGCAGCAGG ATGTCAGCTC ACCAGCCAAG 
ACTGTGGGCC GTTTCTCGGT GGTCAGCACT CAGGACGAGT GGACCCTGGC CTCCCCCCAC 
AGCCTGAGAT ACTCTGCCCC ACCCGACGTC TACCTGGACG AGGCCCCCTC CAGCCCCGAC 
GTGAAGCTGG CAGTGCGGCG GGCGCAGACG GCCTCCTCCA TCGAGGTCGG CGTGGGCGAG 
CCCGTGTCCA GCGACTCTGG GGACGAGGGC CCTCGGGCGA GACCCCCGGT GCAGAAGCAG 
GCGTCCCTGC CCGTGAGTGG CAGCGTGGCT GGCGACTTCG TGAAGAAGGC CACCGCCTTC 
CTGCAGAGGC CTTCTCGGGC CGGCTCGCTG GGCCCCGAGA CACCCAGCAG GGTGGGCATG 
AAGGTCCCCA CGATCAGCGT GACCTCCTTC CATTCCCAGT CGTCCTACAT CAGCAGCGAC 
AATGATTCGG AGCTCGAGGA TGCTGACATA AAGAAGGAGC TGCAGAGTCT GCGGGAGAAG 
CACCTGAAGG AGATCTCGGA GCTGCAGAGC CAGCAGAAGC AGGAGATCGA AGCTCTGTAC 
CGCCGCCTGG GCAAGCCACT GCCCCCCAAC GTGGGCTTCT TCCACACGGC ACCCCCCACT 
GGCCGCCGGA GAAAAACCAG CAAGAGCAAG CTGAAGGCAG GCAAGCTGCT AAATCCCCTG 
GTGCGGCAGC TCAAGGTCGT GGCCTCCAGC ACAGGTCACT TGGCTGACTC CAGCAGAGGC 
CCTCCCGCTA AGGACCCTGC CCAAGCCAGT GTGGGGCTCA CTGCAGACAG CACGGGCCTG 
AGCGGGAAGG CAGTGCAGAC CCAGCAGCCC TGCTCCGTCC GGGCCTCCCT GTCTTCGGAC 
ATCTGCTCCG GCTTAGCCAG TGATGGAGGC GGAGCGCGTG GCCAAGGCTG GACGGTTTAC 
CACCCAACGT CTGAGAGAGT GACCTATAAG TCTAGTAGCA AACCTCGTGC TCGATTCCTC 
AGTGGACCCG TATCTGTGTC CATCTGGTCT GCCCTGAAGC GTCTCTGCCT AGGCAAAGAA 
CACAGCAGTA GGTCCTCCAC CAGCAGCCTG GCCCCAGGCC CTGAGCCAGG CCCCCAGCCC 
GCCCTGCACG TCCAGGCGCA GGTGAACAAC AGCAACAACA AGAAGGGTAC CTTCACGGAC 
GACCTGCACA AGCTGGTGGA CGAGTGGACG AGCAAGACGG TGGGGGCCGC GCAGCTGAAG 
CCCACGCTCA ACCAGCTGAA GCAGACCCAG AAGCTGCAAG ACATGGAGGC CCAGGCAGGC 
TGGGCTGCCC CTGGCGAGGC GCGGGCTATG ACCGCACCTC GAGCAGGAGT GGGGATGCCA 
CGTCTGCCCC CAGCGCCCGG CCCTCTGTCC ACCACGGTCA TTCCCGGAGC CGCCCCGACC 
CTGTCCGTGC CCACACCAGA TGGCGCCCTC GGAACCGCCC GGAGAAACCA GGTGTGGTTT 
GGCCTCCGAG TCCCCCCCAC CGCCTGCTGT GGGCACAGCA CTCAGCCGCG AGGGGGACAG 
CGGGTGGGCA GCAAGACTGC TTCCTTTGCG GCTTCAGACC CTGTTCGCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269323.1
CDS	1..6894
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQGPCRGLTLPCLPWRRAACGAVFLSLFSAESAQSKQPPDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	NM_001282394.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282394.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGGTCCATG CAGGGGGCTC ACCCTGCCCT GTCTCCCATG GCGCAGAGCA 
GCCTGTGGGG CTGTGTTCCT ATCCTTGTTT TCTGCTGAAT CAGCTCAGTC AAAGCAGCCT 
CCAGATTCAG CGCCCTATAA AGACCAGCTG TCCTCGAAGG AACAACCCAG CTTTCTAGCC 
AGTCAGCAGC TCCTGAGCCA GGCGGGCCCC AGCAACCCTC CTGGGGCACC CCCAGCCCCT 
TTGGCCCCCT CCTCCCCTCC TGTGACTGCT CTGCCCCAAG ATGGAGCAGC TCCAGCCACC 
AGCACCATGC CAGAGCCAGC GTCAGGAACT GCCAGCCAGG CAGGGGGTCC AGGGACACCT 
CAGGGGCTGA CCAGTGAGCT CGAGACGTCT CAGCCACTAG CGGAGACTCA CGAGGCCCCG 
CTTGCTGTGC AGCCCCTCGT GGTGGGCCTA GCACCTTGCA CTCCAGCTCC AGAGGCTGCC 
TCAACCAGGG ACGCCAGTGC CCCAAGGGAG CCCCTGCCAC CTCCTGCACC TGAGCCCAGC 
CCCCACAGCG GGACCCCACA GCCCGCCTTG GGTCAACCTG CTCCCCTGCT TCCTGCCGCA 
GTGGGGGCCG TCAGCCTGGC CACCTCCCAG CTCCCAAGCC CACCCCTGGG GCCCACCGTC 
CCCCCACAGC CACCCTCGGC CCTGGAGTCG GATGGGGAAG GGCCGCCCCC CAGGGTGGGC 
TTTGTGGACA GCACCATCAA GAGCCTGGAC GAGAAGCTGC GGACTCTGCT CTACCAGGAG 
CACGTGCCCA CCTCCTCAGC CTCAGCTGGG ACCCCTGTGG AGGTGGGCGA CAGAGACTTC 
ACCCTGGAGC CCCTGAGAGG GGACCAGCCC CGCTCAGAGG TCTGCGGGGG GGACCTGGCC 
CTGCCCCCAG TGCCTAAGGA GGCGGTCTCA GGGCGTGTCC AGCTGCCCCA GCCCTTGGTG 
GAGAAGTCAG AACTGGCCCC CACTCGAGGG GCCGTGATGG AGCAGGGCAC GTCCTCGTCA 
ATGACAGCAG AGTCGTCTCC CAGGAGTATG CTAGGCTATG ACAGAGATGG AAGGCAGGTG 
GCCTCAGACT CCCATGTGGT CCCCAGCGTC CCCCAGGATG TACCTGCTTT TGTGAGACCT 
GCACGTGTGG AGCCCACAGA CAGGGATGGT GGAGAAGCTG GAGAAAGCTC GGCAGAGCCC 
CCGCCGAGTG ACATGGGCAC AGTGGGGGGC CAGGCTAGCC ACCCCCAGAC ACTCGGCGCT 
CGAGCTTTGG GGTCCCCTCG GAAACGTCCA GAGCAGCAGG ATGTCAGCTC ACCAGCCAAG 
ACTGTGGGCC GTTTCTCGGT GGTCAGCACT CAGGACGAGT GGACCCTGGC CTCCCCCCAC 
AGCCTGAGAT ACTCTGCCCC ACCCGACGTC TACCTGGACG AGGCCCCCTC CAGCCCCGAC 
GTGAAGCTGG CAGTGCGGCG GGCGCAGACG GCCTCCTCCA TCGAGGTCGG CGTGGGCGAG 
CCCGTGTCCA GCGACTCTGG GGACGAGGGC CCTCGGGCGA GACCCCCGGT GCAGAAGCAG 
GCGTCCCTGC CCGTGAGTGG CAGCGTGGCT GGCGACTTCG TGAAGAAGGC CACCGCCTTC 
CTGCAGAGGC CTTCTCGGGC CGGCTCGCTG GGCCCCGAGA CACCCAGCAG GGTGGGCATG 
AAGGTCCCCA CGATCAGCGT GACCTCCTTC CATTCCCAGT CGTCCTACAT CAGCAGCGAC 
AATGATTCGG AGCTCGAGGA TGCTGACATA AAGAAGGAGC TGCAGAGTCT GCGGGAGAAG 
CACCTGAAGG AGATCTCGGA GCTGCAGAGC CAGCAGAAGC AGGAGATCGA AGCTCTGTAC 
CGCCGCCTGG GCAAGCCACT GCCCCCCAAC GTGGGCTTCT TCCACACGGC ACCCCCCACT 
GGCCGCCGGA GAAAAACCAG CAAGAGCAAG CTGAAGGCAG GCAAGCTGCT AAATCCCCTG 
GTGCGGCAGC TCAAGGTCGT GGCCTCCAGC ACAGGTCACT TGGCTGACTC CAGCAGAGGC 
CCTCCCGCTA AGGACCCTGC CCAAGCCAGT GTGGGGCTCA CTGCAGACAG CACGGGCCTG 
AGCGGGAAGG CAGTGCAGAC CCAGCAGCCC TGCTCCGTCC GGGCCTCCCT GTCTTCGGAC 
ATCTGCTCCG GCTTAGCCAG TGATGGAGGC GGAGCGCGTG GCCAAGGCTG GACGGTTTAC 
CACCCAACGT CTGAGAGAGT GACCTATAAG TCTAGTAGCA AACCTCGTGC TCGATTCCTC 
AGTGGACCCG TATCTGTGTC CATCTGGTCT GCCCTGAAGC GTCTCTGCCT AGGCAAAGAA 
CACAGCAGTA GGTCCTCCAC CAGCAGCCTG GCCCCAGGCC CTGAGCCAGG CCCCCAGCCC 
GCCCTGCACG TCCAGGCGCA GGTGAACAAC AGCAACAACA AGAAGGGTAC CTTCACGGAC 
GACCTGCACA AGCTGGTGGA CGAGTGGACG AGCAAGACGG TGGGGGCCGC GCAGCTGAAG 
CCCACGCTCA ACCAGCTGAA GCAGACCCAG AAGCTGCAAG ACATGGAGGC CCAGGCAGGC 
TGGGCTGCCC CTGGCGAGGC GCGGGCTATG ACCGCACCTC GAGCAGGAGT GGGGATGCCA 
CGTCTGCCCC CAGCGCCCGG CCCTCTGTCC ACCACGGTCA TTCCCGGAGC CGCCCCGACC 
CTGTCCGTGC CCACACCAGA TGGCGCCCTC GGAACCGCCC GGAGAAACCA GGTGTGGTTT 
GGCCTCCGAG TCCCCCCCAC CGCCTGCTGT GGGCACAGCA CTCAGCCGCG AGGGGGACAG 
CGGGTGGGCA GCAAGACTGC TTCCTTTGCG GCTTCAGACC CTGTTCGCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_005252141.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252198.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X17, mRNA.

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CloneID	OHu00017
Clone ID Related Accession (Same CDS sequence)	NM_006648.3 , XM_005252141.2
Accession Version	XM_005252141.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTATGA CCGCACCTCG AGCAGGAGTG 
GGGATGCCAC GTCTGCCCCC AGCGCCCGGC CCTCTGTCCA CCACGGTCAT TCCCGGAGCC 
GCCCCGACCC TGTCCGTGCC CACACCAGAT CCTGAGAGTG AGAAGCCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252198.1
CDS	233..6886
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDPESEKPD

Target ORF information:

RefSeq Version	XM_005252141.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252141.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTATGA CCGCACCTCG AGCAGGAGTG 
GGGATGCCAC GTCTGCCCCC AGCGCCCGGC CCTCTGTCCA CCACGGTCAT TCCCGGAGCC 
GCCCCGACCC TGTCCGTGCC CACACCAGAT CCTGAGAGTG AGAAGCCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_005252137.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252194.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA.

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CloneID	OHu43440
Clone ID Related Accession (Same CDS sequence)	XM_005252137.3
Accession Version	XM_005252137.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252137.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTATGA CCGCACCTCG AGCAGGAGTG 
GGGATGCCAC GTCTGCCCCC AGCGCCCGGC CCTCTGTCCA CCACGGTCAT TCCCGGAGCC 
GCCCCGACCC TGTCCGTGCC CACACCAGAT GGCGCCCTCG GAACCGCCCG GAGAAACCAG 
GTGTGGTTTG GCCTCCGAGT CCCCCCCACC GCCTGCTGTG GGCACAGCAC TCAGCCGCGA 
GGGGGACAGC GGGTGGGCAG CAAGACTGCT TCCTTTGCGG CTTCAGACCC TGTTCGCTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252194.1
CDS	233..7015
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	XM_005252137.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252137.3

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTATGA CCGCACCTCG AGCAGGAGTG 
GGGATGCCAC GTCTGCCCCC AGCGCCCGGC CCTCTGTCCA CCACGGTCAT TCCCGGAGCC 
GCCCCGACCC TGTCCGTGCC CACACCAGAT GGCGCCCTCG GAACCGCCCG GAGAAACCAG 
GTGTGGTTTG GCCTCCGAGT CCCCCCCACC GCCTGCTGTG GGCACAGCAC TCAGCCGCGA 
GGGGGACAGC GGGTGGGCAG CAAGACTGCT TCCTTTGCGG CTTCAGACCC TGTTCGCTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_005252140.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252197.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X16, mRNA.

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CloneID	OHu43444
Clone ID Related Accession (Same CDS sequence)	XM_005252140.2
Accession Version	XM_005252140.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252140.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTATGA CCGCACCTCG AGCAGGAGTG 
GGGATGCCAC GTCTGCCCCC AGCGCCCGGC CCTCTGTCCA CCACGGTCAT TCCCGGAGCC 
GCCCCGACCC TGTCCGTGCC CACACCAGGT TCCTGTGGTC CACGCGCCGT CTCCACACCC 
ACTTCCTATA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252197.1
CDS	233..6907
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_005252140.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252140.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTATGA CCGCACCTCG AGCAGGAGTG 
GGGATGCCAC GTCTGCCCCC AGCGCCCGGC CCTCTGTCCA CCACGGTCAT TCCCGGAGCC 
GCCCCGACCC TGTCCGTGCC CACACCAGGT TCCTGTGGTC CACGCGCCGT CTCCACACCC 
ACTTCCTATA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_005252144.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252201.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X23, mRNA.

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CloneID	OHu43448
Clone ID Related Accession (Same CDS sequence)	XM_005252144.2
Accession Version	XM_005252144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTCCCC AAGAACTAGG CTTGTCCACT 
TTACAGATTG GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252201.1
CDS	233..6790
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAPQELGLSTLQIGR

Target ORF information:

RefSeq Version	XM_005252144.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252144.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTCCCC AAGAACTAGG CTTGTCCACT 
TTACAGATTG GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518926.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517228.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA.

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CloneID	OHu71647
Clone ID Related Accession (Same CDS sequence)	XM_011518926.2
Accession Version	XM_011518926.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTTCTG TGCGGTTGAC TCCTTTTCCT 
CCCCGCCTGG AGATCCCCGT GGTGTCGACT GGAAGCATGG AGGCACCTTG GGGAGATGAC 
CGCACCTCGA GCAGGAGTGG GGATGCCACG TCTGCCCCCA GCGCCCGGCC CTCTGTCCAC 
CACGGTCATT CCCGGAGCCG CCCCGACCCT GTCCGTGCCC ACACCAGATG GCGCCCTCGG 
AACCGCCCGG AGAAACCAGG TGTGGTTTGG CCTCCGAGTC CCCCCCACCG CCTGCTGTGG 
GCACAGCACT CAGCCGCGAG GGGGACAGCG GGTGGGCAGC AAGACTGCTT CCTTTGCGGC 
TTCAGACCCT GTTCGCTCCT AGGTTCCTGT GGTCCACGCG CCGTCTCCAC ACCCACTTCC 
TATACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517228.1
CDS	233..7141
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518926.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518926.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTTCTG TGCGGTTGAC TCCTTTTCCT 
CCCCGCCTGG AGATCCCCGT GGTGTCGACT GGAAGCATGG AGGCACCTTG GGGAGATGAC 
CGCACCTCGA GCAGGAGTGG GGATGCCACG TCTGCCCCCA GCGCCCGGCC CTCTGTCCAC 
CACGGTCATT CCCGGAGCCG CCCCGACCCT GTCCGTGCCC ACACCAGATG GCGCCCTCGG 
AACCGCCCGG AGAAACCAGG TGTGGTTTGG CCTCCGAGTC CCCCCCACCG CCTGCTGTGG 
GCACAGCACT CAGCCGCGAG GGGGACAGCG GGTGGGCAGC AAGACTGCTT CCTTTGCGGC 
TTCAGACCCT GTTCGCTCCT AGGTTCCTGT GGTCCACGCG CCGTCTCCAC ACCCACTTCC 
TATACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518927.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517229.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA.

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CloneID	OHu71648
Clone ID Related Accession (Same CDS sequence)	XM_011518927.2
Accession Version	XM_011518927.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGCAA GCTGCTAAAT 
CCCCTGGTGC GGCAGCTCAA GGTCGTGGCC TCCAGCACAG GTCACTTGGC TGACTCCAGC 
AGAGGCCCTC CCGCTAAGGA CCCTGCCCAA GCCAGTGTGG GGCTCACTGC AGACAGCACG 
GGCCTGAGCG GGAAGGCAGT GCAGACCCAG CAGCCCTGCT CCGTCCGGGC CTCCCTGTCT 
TCGGACATCT GCTCCGGCTT AGCCAGTGAT GGAGGCGGAG CGCGTGGCCA AGGCTGGACG 
GTTTACCACC CAACGTCTGA GAGAGTGACC TATAAGTCTA GTAGCAAACC TCGTGCTCGA 
TTCCTCAGTG GACCCGTATC TGTGTCCATC TGGTCTGCCC TGAAGCGTCT CTGCCTAGGC 
AAAGAACACA GCAGTAGGTC CTCCACCAGC AGCCTGGCCC CAGGCCCTGA GCCAGGCCCC 
CAGCCCGCCC TGCACGTCCA GGCGCAGGTG AACAACAGCA ACAACAAGAA GGGTACCTTC 
ACGGACGACC TGCACAAGCT GGTGGACGAG TGGACGAGCA AGACGGTGGG GGCCGCGCAG 
CTGAAGCCCA CGCTCAACCA GCTGAAGCAG ACCCAGAAGC TGCAAGACAT GGAGGCCCAG 
GCAGGCTGGG CTGCCCCTGG CGAGGCGCGG GCTTCTGTGC GGTTGACTCC TTTTCCTCCC 
CGCCTGGAGA TCCCCGTGGT GTCGACTGGA AGCATGGAGG CACCTTGGGG AGATGACCGC 
ACCTCGAGCA GGAGTGGGGA TGCCACGTCT GCCCCCAGCG CCCGGCCCTC TGTCCACCAC 
GGTCATTCCC GGAGCCGCCC CGACCCTGTC CGTGCCCACA CCAGATGGCG CCCTCGGAAC 
CGCCCGGAGA AACCAGGTGT GGTTTGGCCT CCGAGTCCCC CCCACCGCCT GCTGTGGGCA 
CAGCACTCAG CCGCGAGGGG GACAGCGGGT GGGCAGCAAG ACTGCTTCCT TTGCGGCTTC 
AGACCCTGTT CGCTCCTAGG TTCCTGTGGT CCACGCGCCG TCTCCACACC CACTTCCTAT 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517229.1
CDS	233..7138
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518927.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518927.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGCAA GCTGCTAAAT 
CCCCTGGTGC GGCAGCTCAA GGTCGTGGCC TCCAGCACAG GTCACTTGGC TGACTCCAGC 
AGAGGCCCTC CCGCTAAGGA CCCTGCCCAA GCCAGTGTGG GGCTCACTGC AGACAGCACG 
GGCCTGAGCG GGAAGGCAGT GCAGACCCAG CAGCCCTGCT CCGTCCGGGC CTCCCTGTCT 
TCGGACATCT GCTCCGGCTT AGCCAGTGAT GGAGGCGGAG CGCGTGGCCA AGGCTGGACG 
GTTTACCACC CAACGTCTGA GAGAGTGACC TATAAGTCTA GTAGCAAACC TCGTGCTCGA 
TTCCTCAGTG GACCCGTATC TGTGTCCATC TGGTCTGCCC TGAAGCGTCT CTGCCTAGGC 
AAAGAACACA GCAGTAGGTC CTCCACCAGC AGCCTGGCCC CAGGCCCTGA GCCAGGCCCC 
CAGCCCGCCC TGCACGTCCA GGCGCAGGTG AACAACAGCA ACAACAAGAA GGGTACCTTC 
ACGGACGACC TGCACAAGCT GGTGGACGAG TGGACGAGCA AGACGGTGGG GGCCGCGCAG 
CTGAAGCCCA CGCTCAACCA GCTGAAGCAG ACCCAGAAGC TGCAAGACAT GGAGGCCCAG 
GCAGGCTGGG CTGCCCCTGG CGAGGCGCGG GCTTCTGTGC GGTTGACTCC TTTTCCTCCC 
CGCCTGGAGA TCCCCGTGGT GTCGACTGGA AGCATGGAGG CACCTTGGGG AGATGACCGC 
ACCTCGAGCA GGAGTGGGGA TGCCACGTCT GCCCCCAGCG CCCGGCCCTC TGTCCACCAC 
GGTCATTCCC GGAGCCGCCC CGACCCTGTC CGTGCCCACA CCAGATGGCG CCCTCGGAAC 
CGCCCGGAGA AACCAGGTGT GGTTTGGCCT CCGAGTCCCC CCCACCGCCT GCTGTGGGCA 
CAGCACTCAG CCGCGAGGGG GACAGCGGGT GGGCAGCAAG ACTGCTTCCT TTGCGGCTTC 
AGACCCTGTT CGCTCCTAGG TTCCTGTGGT CCACGCGCCG TCTCCACACC CACTTCCTAT 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518928.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517230.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA.

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CloneID	OHu71649
Clone ID Related Accession (Same CDS sequence)	XM_011518928.2
Accession Version	XM_011518928.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG TCACTTGGCT 
GACTCCAGCA GAGGCCCTCC CGCTAAGGAC CCTGCCCAAG CCAGTGTGGG GCTCACTGCA 
GACAGCACGG GCCTGAGCGG GAAGGCAGTG CAGACCCAGC AGCCCTGCTC CGTCCGGGCC 
TCCCTGTCTT CGGACATCTG CTCCGGCTTA GCCAGTGATG GAGGCGGAGC GCGTGGCCAA 
GGCTGGACGG TTTACCACCC AACGTCTGAG AGAGTGACCT ATAAGTCTAG TAGCAAACCT 
CGTGCTCGAT TCCTCAGTGG ACCCGTATCT GTGTCCATCT GGTCTGCCCT GAAGCGTCTC 
TGCCTAGGCA AAGAACACAG CAGTAGGTCC TCCACCAGCA GCCTGGCCCC AGGCCCTGAG 
CCAGGCCCCC AGCCCGCCCT GCACGTCCAG GCGCAGGTGA ACAACAGCAA CAACAAGAAG 
GGTACCTTCA CGGACGACCT GCACAAGCTG GTGGACGAGT GGACGAGCAA GACGGTGGGG 
GCCGCGCAGC TGAAGCCCAC GCTCAACCAG CTGAAGCAGA CCCAGAAGCT GCAAGACATG 
GAGGCCCAGG CAGGCTGGGC TGCCCCTGGC GAGGCGCGGG CTTCTGTGCG GTTGACTCCT 
TTTCCTCCCC GCCTGGAGAT CCCCGTGGTG TCGACTGGAA GCATGGAGGC ACCTTGGGGA 
GATGACCGCA CCTCGAGCAG GAGTGGGGAT GCCACGTCTG CCCCCAGCGC CCGGCCCTCT 
GTCCACCACG GTCATTCCCG GAGCCGCCCC GACCCTGTCC GTGCCCACAC CAGATGGCGC 
CCTCGGAACC GCCCGGAGAA ACCAGGTGTG GTTTGGCCTC CGAGTCCCCC CCACCGCCTG 
CTGTGGGCAC AGCACTCAGC CGCGAGGGGG ACAGCGGGTG GGCAGCAAGA CTGCTTCCTT 
TGCGGCTTCA GACCCTGTTC GCTCCTAGGT TCCTGTGGTC CACGCGCCGT CTCCACACCC 
ACTTCCTATA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517230.1
CDS	233..7087
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518928.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518928.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG TCACTTGGCT 
GACTCCAGCA GAGGCCCTCC CGCTAAGGAC CCTGCCCAAG CCAGTGTGGG GCTCACTGCA 
GACAGCACGG GCCTGAGCGG GAAGGCAGTG CAGACCCAGC AGCCCTGCTC CGTCCGGGCC 
TCCCTGTCTT CGGACATCTG CTCCGGCTTA GCCAGTGATG GAGGCGGAGC GCGTGGCCAA 
GGCTGGACGG TTTACCACCC AACGTCTGAG AGAGTGACCT ATAAGTCTAG TAGCAAACCT 
CGTGCTCGAT TCCTCAGTGG ACCCGTATCT GTGTCCATCT GGTCTGCCCT GAAGCGTCTC 
TGCCTAGGCA AAGAACACAG CAGTAGGTCC TCCACCAGCA GCCTGGCCCC AGGCCCTGAG 
CCAGGCCCCC AGCCCGCCCT GCACGTCCAG GCGCAGGTGA ACAACAGCAA CAACAAGAAG 
GGTACCTTCA CGGACGACCT GCACAAGCTG GTGGACGAGT GGACGAGCAA GACGGTGGGG 
GCCGCGCAGC TGAAGCCCAC GCTCAACCAG CTGAAGCAGA CCCAGAAGCT GCAAGACATG 
GAGGCCCAGG CAGGCTGGGC TGCCCCTGGC GAGGCGCGGG CTTCTGTGCG GTTGACTCCT 
TTTCCTCCCC GCCTGGAGAT CCCCGTGGTG TCGACTGGAA GCATGGAGGC ACCTTGGGGA 
GATGACCGCA CCTCGAGCAG GAGTGGGGAT GCCACGTCTG CCCCCAGCGC CCGGCCCTCT 
GTCCACCACG GTCATTCCCG GAGCCGCCCC GACCCTGTCC GTGCCCACAC CAGATGGCGC 
CCTCGGAACC GCCCGGAGAA ACCAGGTGTG GTTTGGCCTC CGAGTCCCCC CCACCGCCTG 
CTGTGGGCAC AGCACTCAGC CGCGAGGGGG ACAGCGGGTG GGCAGCAAGA CTGCTTCCTT 
TGCGGCTTCA GACCCTGTTC GCTCCTAGGT TCCTGTGGTC CACGCGCCGT CTCCACACCC 
ACTTCCTATA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518929.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517231.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA.

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CloneID	OHu71650
Clone ID Related Accession (Same CDS sequence)	XM_011518929.2
Accession Version	XM_011518929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGGTCT 
GCCCTGAAGC GTCTCTGCCT AGGCAAAGAA CACAGCAGTA GGTCCTCCAC CAGCAGCCTG 
GCCCCAGGCC CTGAGCCAGG CCCCCAGCCC GCCCTGCACG TCCAGGCGCA GGTGAACAAC 
AGCAACAACA AGAAGGGTAC CTTCACGGAC GACCTGCACA AGCTGGTGGA CGAGTGGACG 
AGCAAGACGG TGGGGGCCGC GCAGCTGAAG CCCACGCTCA ACCAGCTGAA GCAGACCCAG 
AAGCTGCAAG ACATGGAGGC CCAGGCAGGC TGGGCTGCCC CTGGCGAGGC GCGGGCTTCT 
GTGCGGTTGA CTCCTTTTCC TCCCCGCCTG GAGATCCCCG TGGTGTCGAC TGGAAGCATG 
GAGGCACCTT GGGGAGATGA CCGCACCTCG AGCAGGAGTG GGGATGCCAC GTCTGCCCCC 
AGCGCCCGGC CCTCTGTCCA CCACGGTCAT TCCCGGAGCC GCCCCGACCC TGTCCGTGCC 
CACACCAGAT GGCGCCCTCG GAACCGCCCG GAGAAACCAG GTGTGGTTTG GCCTCCGAGT 
CCCCCCCACC GCCTGCTGTG GGCACAGCAC TCAGCCGCGA GGGGGACAGC GGGTGGGCAG 
CAAGACTGCT TCCTTTGCGG CTTCAGACCC TGTTCGCTCC TAGGTTCCTG TGGTCCACGC 
GCCGTCTCCA CACCCACTTC CTATACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517231.1
CDS	233..7042
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518929.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518929.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGGTCT 
GCCCTGAAGC GTCTCTGCCT AGGCAAAGAA CACAGCAGTA GGTCCTCCAC CAGCAGCCTG 
GCCCCAGGCC CTGAGCCAGG CCCCCAGCCC GCCCTGCACG TCCAGGCGCA GGTGAACAAC 
AGCAACAACA AGAAGGGTAC CTTCACGGAC GACCTGCACA AGCTGGTGGA CGAGTGGACG 
AGCAAGACGG TGGGGGCCGC GCAGCTGAAG CCCACGCTCA ACCAGCTGAA GCAGACCCAG 
AAGCTGCAAG ACATGGAGGC CCAGGCAGGC TGGGCTGCCC CTGGCGAGGC GCGGGCTTCT 
GTGCGGTTGA CTCCTTTTCC TCCCCGCCTG GAGATCCCCG TGGTGTCGAC TGGAAGCATG 
GAGGCACCTT GGGGAGATGA CCGCACCTCG AGCAGGAGTG GGGATGCCAC GTCTGCCCCC 
AGCGCCCGGC CCTCTGTCCA CCACGGTCAT TCCCGGAGCC GCCCCGACCC TGTCCGTGCC 
CACACCAGAT GGCGCCCTCG GAACCGCCCG GAGAAACCAG GTGTGGTTTG GCCTCCGAGT 
CCCCCCCACC GCCTGCTGTG GGCACAGCAC TCAGCCGCGA GGGGGACAGC GGGTGGGCAG 
CAAGACTGCT TCCTTTGCGG CTTCAGACCC TGTTCGCTCC TAGGTTCCTG TGGTCCACGC 
GCCGTCTCCA CACCCACTTC CTATACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518930.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517232.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA.

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CloneID	OHu71651
Clone ID Related Accession (Same CDS sequence)	XM_011518930.2
Accession Version	XM_011518930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGAGAAG 
TCAGAACTGG CCCCCACTCG AGGGGCCGTG ATGGAGCAGG GCACGTCCTC GTCAATGACA 
GCAGAGTCGT CTCCCAGGAG TATGCTAGGC TATGACAGAG ATGGAAGGCA GGTGGCCTCA 
GACTCCCATG TGGTCCCCAG CGTCCCCCAG GATGTACCTG CTTTTGTGAG ACCTGCACGT 
GTGGAGCCCA CAGACAGGGA TGGTGGAGAA GCTGGAGAAA GCTCGGCAGA GCCCCCGCCG 
AGTGACATGG GCACAGTGGG GGGCCAGGCT AGCCACCCCC AGACACTCGG CGCTCGAGCT 
TTGGGGTCCC CTCGGAAACG TCCAGAGCAG CAGGATGTCA GCTCACCAGC CAAGACTGTG 
GGCCGTTTCT CGGTGGTCAG CACTCAGGAC GAGTGGACCC TGGCCTCCCC CCACAGCCTG 
AGATACTCTG CCCCACCCGA CGTCTACCTG GACGAGGCCC CCTCCAGCCC CGACGTGAAG 
CTGGCAGTGC GGCGGGCGCA GACGGCCTCC TCCATCGAGG TCGGCGTGGG CGAGCCCGTG 
TCCAGCGACT CTGGGGACGA GGGCCCTCGG GCGAGACCCC CGGTGCAGAA GCAGGCGTCC 
CTGCCCGTGA GTGGCAGCGT GGCTGGCGAC TTCGTGAAGA AGGCCACCGC CTTCCTGCAG 
AGGCCTTCTC GGGCCGGCTC GCTGGGCCCC GAGACACCCA GCAGGGTGGG CATGAAGGTC 
CCCACGATCA GCGTGACCTC CTTCCATTCC CAGTCGTCCT ACATCAGCAG CGACAATGAT 
TCGGAGCTCG AGGATGCTGA CATAAAGAAG GAGCTGCAGA GTCTGCGGGA GAAGCACCTG 
AAGGAGATCT CGGAGCTGCA GAGCCAGCAG AAGCAGGAGA TCGAAGCTCT GTACCGCCGC 
CTGGGCAAGC CACTGCCCCC CAACGTGGGC TTCTTCCACA CGGCACCCCC CACTGGCCGC 
CGGAGAAAAA CCAGCAAGAG CAAGCTGAAG GCAGGCAAGC TGCTAAATCC CCTGGTGCGG 
CAGCTCAAGG TCGTGGCCTC CAGCACAGGT CACTTGGCTG ACTCCAGCAG AGGCCCTCCC 
GCTAAGGACC CTGCCCAAGC CAGTGTGGGG CTCACTGCAG ACAGCACGGG CCTGAGCGGG 
AAGGCAGTGC AGACCCAGCA GCCCTGCTCC GTCCGGGCCT CCCTGTCTTC GGACATCTGC 
TCCGGCTTAG CCAGTGATGG AGGCGGAGCG CGTGGCCAAG GCTGGACGGT TTACCACCCA 
ACGTCTGAGA GAGTGACCTA TAAGTCTAGT AGCAAACCTC GTGCTCGATT CCTCAGTGGA 
CCCGTATCTG TGTCCATCTG GTCTGCCCTG AAGCGTCTCT GCCTAGGCAA AGAACACAGC 
AGTAGGTCCT CCACCAGCAG CCTGGCCCCA GGCCCTGAGC CAGGCCCCCA GCCCGCCCTG 
CACGTCCAGG CGCAGGTGAA CAACAGCAAC AACAAGAAGG GTACCTTCAC GGACGACCTG 
CACAAGCTGG TGGACGAGTG GACGAGCAAG ACGGTGGGGG CCGCGCAGCT GAAGCCCACG 
CTCAACCAGC TGAAGCAGAC CCAGAAGCTG CAAGACATGG AGGCCCAGGC AGGCTGGGCT 
GCCCCTGGCG AGGCGCGGGC TTCTGTGCGG TTGACTCCTT TTCCTCCCCG CCTGGAGATC 
CCCGTGGTGT CGACTGGAAG CATGGAGGCA CCTTGGGGAG ATGACCGCAC CTCGAGCAGG 
AGTGGGGATG CCACGTCTGC CCCCAGCGCC CGGCCCTCTG TCCACCACGG TCATTCCCGG 
AGCCGCCCCG ACCCTGTCCG TGCCCACACC AGATGGCGCC CTCGGAACCG CCCGGAGAAA 
CCAGGTGTGG TTTGGCCTCC GAGTCCCCCC CACCGCCTGC TGTGGGCACA GCACTCAGCC 
GCGAGGGGGA CAGCGGGTGG GCAGCAAGAC TGCTTCCTTT GCGGCTTCAG ACCCTGTTCG 
CTCCTAGGTT CCTGTGGTCC ACGCGCCGTC TCCACACCCA CTTCCTATAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517232.1
CDS	233..7006
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518930.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518930.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGAGAAG 
TCAGAACTGG CCCCCACTCG AGGGGCCGTG ATGGAGCAGG GCACGTCCTC GTCAATGACA 
GCAGAGTCGT CTCCCAGGAG TATGCTAGGC TATGACAGAG ATGGAAGGCA GGTGGCCTCA 
GACTCCCATG TGGTCCCCAG CGTCCCCCAG GATGTACCTG CTTTTGTGAG ACCTGCACGT 
GTGGAGCCCA CAGACAGGGA TGGTGGAGAA GCTGGAGAAA GCTCGGCAGA GCCCCCGCCG 
AGTGACATGG GCACAGTGGG GGGCCAGGCT AGCCACCCCC AGACACTCGG CGCTCGAGCT 
TTGGGGTCCC CTCGGAAACG TCCAGAGCAG CAGGATGTCA GCTCACCAGC CAAGACTGTG 
GGCCGTTTCT CGGTGGTCAG CACTCAGGAC GAGTGGACCC TGGCCTCCCC CCACAGCCTG 
AGATACTCTG CCCCACCCGA CGTCTACCTG GACGAGGCCC CCTCCAGCCC CGACGTGAAG 
CTGGCAGTGC GGCGGGCGCA GACGGCCTCC TCCATCGAGG TCGGCGTGGG CGAGCCCGTG 
TCCAGCGACT CTGGGGACGA GGGCCCTCGG GCGAGACCCC CGGTGCAGAA GCAGGCGTCC 
CTGCCCGTGA GTGGCAGCGT GGCTGGCGAC TTCGTGAAGA AGGCCACCGC CTTCCTGCAG 
AGGCCTTCTC GGGCCGGCTC GCTGGGCCCC GAGACACCCA GCAGGGTGGG CATGAAGGTC 
CCCACGATCA GCGTGACCTC CTTCCATTCC CAGTCGTCCT ACATCAGCAG CGACAATGAT 
TCGGAGCTCG AGGATGCTGA CATAAAGAAG GAGCTGCAGA GTCTGCGGGA GAAGCACCTG 
AAGGAGATCT CGGAGCTGCA GAGCCAGCAG AAGCAGGAGA TCGAAGCTCT GTACCGCCGC 
CTGGGCAAGC CACTGCCCCC CAACGTGGGC TTCTTCCACA CGGCACCCCC CACTGGCCGC 
CGGAGAAAAA CCAGCAAGAG CAAGCTGAAG GCAGGCAAGC TGCTAAATCC CCTGGTGCGG 
CAGCTCAAGG TCGTGGCCTC CAGCACAGGT CACTTGGCTG ACTCCAGCAG AGGCCCTCCC 
GCTAAGGACC CTGCCCAAGC CAGTGTGGGG CTCACTGCAG ACAGCACGGG CCTGAGCGGG 
AAGGCAGTGC AGACCCAGCA GCCCTGCTCC GTCCGGGCCT CCCTGTCTTC GGACATCTGC 
TCCGGCTTAG CCAGTGATGG AGGCGGAGCG CGTGGCCAAG GCTGGACGGT TTACCACCCA 
ACGTCTGAGA GAGTGACCTA TAAGTCTAGT AGCAAACCTC GTGCTCGATT CCTCAGTGGA 
CCCGTATCTG TGTCCATCTG GTCTGCCCTG AAGCGTCTCT GCCTAGGCAA AGAACACAGC 
AGTAGGTCCT CCACCAGCAG CCTGGCCCCA GGCCCTGAGC CAGGCCCCCA GCCCGCCCTG 
CACGTCCAGG CGCAGGTGAA CAACAGCAAC AACAAGAAGG GTACCTTCAC GGACGACCTG 
CACAAGCTGG TGGACGAGTG GACGAGCAAG ACGGTGGGGG CCGCGCAGCT GAAGCCCACG 
CTCAACCAGC TGAAGCAGAC CCAGAAGCTG CAAGACATGG AGGCCCAGGC AGGCTGGGCT 
GCCCCTGGCG AGGCGCGGGC TTCTGTGCGG TTGACTCCTT TTCCTCCCCG CCTGGAGATC 
CCCGTGGTGT CGACTGGAAG CATGGAGGCA CCTTGGGGAG ATGACCGCAC CTCGAGCAGG 
AGTGGGGATG CCACGTCTGC CCCCAGCGCC CGGCCCTCTG TCCACCACGG TCATTCCCGG 
AGCCGCCCCG ACCCTGTCCG TGCCCACACC AGATGGCGCC CTCGGAACCG CCCGGAGAAA 
CCAGGTGTGG TTTGGCCTCC GAGTCCCCCC CACCGCCTGC TGTGGGCACA GCACTCAGCC 
GCGAGGGGGA CAGCGGGTGG GCAGCAAGAC TGCTTCCTTT GCGGCTTCAG ACCCTGTTCG 
CTCCTAGGTT CCTGTGGTCC ACGCGCCGTC TCCACACCCA CTTCCTATAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518931.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517233.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X9, mRNA.

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CloneID	OHu71652
Clone ID Related Accession (Same CDS sequence)	XM_011518931.2
Accession Version	XM_011518931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGGTCC 
TCCACCAGCA GCCTGGCCCC AGGCCCTGAG CCAGGCCCCC AGCCCGCCCT GCACGTCCAG 
GCGCAGGTGA ACAACAGCAA CAACAAGAAG GGTACCTTCA CGGACGACCT GCACAAGCTG 
GTGGACGAGT GGACGAGCAA GACGGTGGGG GCCGCGCAGC TGAAGCCCAC GCTCAACCAG 
CTGAAGCAGA CCCAGAAGCT GCAAGACATG GAGGCCCAGG CAGGCTGGGC TGCCCCTGGC 
GAGGCGCGGG CTTCTGTGCG GTTGACTCCT TTTCCTCCCC GCCTGGAGAT CCCCGTGGTG 
TCGACTGGAA GCATGGAGGC ACCTTGGGGA GATGACCGCA CCTCGAGCAG GAGTGGGGAT 
GCCACGTCTG CCCCCAGCGC CCGGCCCTCT GTCCACCACG GTCATTCCCG GAGCCGCCCC 
GACCCTGTCC GTGCCCACAC CAGATGGCGC CCTCGGAACC GCCCGGAGAA ACCAGGTGTG 
GTTTGGCCTC CGAGTCCCCC CCACCGCCTG CTGTGGGCAC AGCACTCAGC CGCGAGGGGG 
ACAGCGGGTG GGCAGCAAGA CTGCTTCCTT TGCGGCTTCA GACCCTGTTC GCTCCTAGGT 
TCCTGTGGTC CACGCGCCGT CTCCACACCC ACTTCCTATA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517233.1
CDS	233..6997
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518931.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518931.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGGTCC 
TCCACCAGCA GCCTGGCCCC AGGCCCTGAG CCAGGCCCCC AGCCCGCCCT GCACGTCCAG 
GCGCAGGTGA ACAACAGCAA CAACAAGAAG GGTACCTTCA CGGACGACCT GCACAAGCTG 
GTGGACGAGT GGACGAGCAA GACGGTGGGG GCCGCGCAGC TGAAGCCCAC GCTCAACCAG 
CTGAAGCAGA CCCAGAAGCT GCAAGACATG GAGGCCCAGG CAGGCTGGGC TGCCCCTGGC 
GAGGCGCGGG CTTCTGTGCG GTTGACTCCT TTTCCTCCCC GCCTGGAGAT CCCCGTGGTG 
TCGACTGGAA GCATGGAGGC ACCTTGGGGA GATGACCGCA CCTCGAGCAG GAGTGGGGAT 
GCCACGTCTG CCCCCAGCGC CCGGCCCTCT GTCCACCACG GTCATTCCCG GAGCCGCCCC 
GACCCTGTCC GTGCCCACAC CAGATGGCGC CCTCGGAACC GCCCGGAGAA ACCAGGTGTG 
GTTTGGCCTC CGAGTCCCCC CCACCGCCTG CTGTGGGCAC AGCACTCAGC CGCGAGGGGG 
ACAGCGGGTG GGCAGCAAGA CTGCTTCCTT TGCGGCTTCA GACCCTGTTC GCTCCTAGGT 
TCCTGTGGTC CACGCGCCGT CTCCACACCC ACTTCCTATA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518932.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517234.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X10, mRNA.

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CloneID	OHu71653
Clone ID Related Accession (Same CDS sequence)	XM_011518932.2
Accession Version	XM_011518932.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518932.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTTCTG TGCGGTTGAC TCCTTTTCCT 
CCCCGCCTGG AGATCCCCGT GGTGTCGACT GGAAGCATGG AGGCACCTTG GGGAGATGAC 
CGCACCTCGA GCAGGAGTGG GGATGCCACG TCTGCCCCCA GCGCCCGGCC CTCTGTCCAC 
CACGGTCATT CCCGGAGCCG CCCCGACCCT GTCCGTGCCC ACACCAGGTT CCTGTGGTCC 
ACGCGCCGTC TCCACACCCA CTTCCTATAC TTGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517234.1
CDS	233..6991
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRFLWSTRRLHTHFLYLS

Target ORF information:

RefSeq Version	XM_011518932.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518932.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGCTGG 
ACGGTTTACC ACCCAACGTC TGAGAGAGTG ACCTATAAGT CTAGTAGCAA ACCTCGTGCT 
CGATTCCTCA GTGGACCCGT ATCTGTGTCC ATCTGGTCTG CCCTGAAGCG TCTCTGCCTA 
GGCAAAGAAC ACAGCAGTAG GTCCTCCACC AGCAGCCTGG CCCCAGGCCC TGAGCCAGGC 
CCCCAGCCCG CCCTGCACGT CCAGGCGCAG GTGAACAACA GCAACAACAA GAAGGGTACC 
TTCACGGACG ACCTGCACAA GCTGGTGGAC GAGTGGACGA GCAAGACGGT GGGGGCCGCG 
CAGCTGAAGC CCACGCTCAA CCAGCTGAAG CAGACCCAGA AGCTGCAAGA CATGGAGGCC 
CAGGCAGGCT GGGCTGCCCC TGGCGAGGCG CGGGCTTCTG TGCGGTTGAC TCCTTTTCCT 
CCCCGCCTGG AGATCCCCGT GGTGTCGACT GGAAGCATGG AGGCACCTTG GGGAGATGAC 
CGCACCTCGA GCAGGAGTGG GGATGCCACG TCTGCCCCCA GCGCCCGGCC CTCTGTCCAC 
CACGGTCATT CCCGGAGCCG CCCCGACCCT GTCCGTGCCC ACACCAGGTT CCTGTGGTCC 
ACGCGCCGTC TCCACACCCA CTTCCTATAC TTGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518935.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517237.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X21, mRNA.

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CloneID	OHu71656
Clone ID Related Accession (Same CDS sequence)	XM_011518935.2
Accession Version	XM_011518935.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518935.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGGTCTGC CCTGAAGCGT 
CTCTGCCTAG GCAAAGAACA CAGCAGTAGG TCCTCCACCA GCAGCCTGGC CCCAGGCCCT 
GAGCCAGGCC CCCAGCCCGC CCTGCACGTC CAGGCGCAGG TGAACAACAG CAACAACAAG 
AAGGGTACCT TCACGGACGA CCTGCACAAG CTGGTGGACG AGTGGACGAG CAAGACGGTG 
GGGGCCGCGC AGCTGAAGCC CACGCTCAAC CAGCTGAAGC AGACCCAGAA GCTGCAAGAC 
ATGGAGGCCC AGGCAGGCTG GGCTGCCCCT GGCGAGGCGC GGGCTTCTGT GCGGTTGACT 
CCTTTTCCTC CCCGCCTGGA GATCCCCGTG GTGTCGACTG GAAGCATGGA GGCACCTTGG 
GGAGATGACC GCACCTCGAG CAGGAGTGGG GATGCCACGT CTGCCCCCAG CGCCCGGCCC 
TCTGTCCACC ACGGTCATTC CCGGAGCCGC CCCGACCCTG TCCGTGCCCA CACCAGATGG 
CGCCCTCGGA ACCGCCCGGA GAAACCAGGT GTGGTTTGGC CTCCGAGTCC CCCCCACCGC 
CTGCTGTGGG CACAGCACTC AGCCGCGAGG GGGACAGCGG GTGGGCAGCA AGACTGCTTC 
CTTTGCGGCT TCAGACCCTG TTCGCTCCTA GGTTCCTGTG GTCCACGCGC CGTCTCCACA 
CCCACTTCCT ATACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517237.1
CDS	233..6850
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518935.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518935.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGGTCTGC CCTGAAGCGT 
CTCTGCCTAG GCAAAGAACA CAGCAGTAGG TCCTCCACCA GCAGCCTGGC CCCAGGCCCT 
GAGCCAGGCC CCCAGCCCGC CCTGCACGTC CAGGCGCAGG TGAACAACAG CAACAACAAG 
AAGGGTACCT TCACGGACGA CCTGCACAAG CTGGTGGACG AGTGGACGAG CAAGACGGTG 
GGGGCCGCGC AGCTGAAGCC CACGCTCAAC CAGCTGAAGC AGACCCAGAA GCTGCAAGAC 
ATGGAGGCCC AGGCAGGCTG GGCTGCCCCT GGCGAGGCGC GGGCTTCTGT GCGGTTGACT 
CCTTTTCCTC CCCGCCTGGA GATCCCCGTG GTGTCGACTG GAAGCATGGA GGCACCTTGG 
GGAGATGACC GCACCTCGAG CAGGAGTGGG GATGCCACGT CTGCCCCCAG CGCCCGGCCC 
TCTGTCCACC ACGGTCATTC CCGGAGCCGC CCCGACCCTG TCCGTGCCCA CACCAGATGG 
CGCCCTCGGA ACCGCCCGGA GAAACCAGGT GTGGTTTGGC CTCCGAGTCC CCCCCACCGC 
CTGCTGTGGG CACAGCACTC AGCCGCGAGG GGGACAGCGG GTGGGCAGCA AGACTGCTTC 
CTTTGCGGCT TCAGACCCTG TTCGCTCCTA GGTTCCTGTG GTCCACGCGC CGTCTCCACA 
CCCACTTCCT ATACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518936.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517238.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X22, mRNA.

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CloneID	OHu71657
Clone ID Related Accession (Same CDS sequence)	XM_011518936.2
Accession Version	XM_011518936.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGGTCCTC CACCAGCAGC 
CTGGCCCCAG GCCCTGAGCC AGGCCCCCAG CCCGCCCTGC ACGTCCAGGC GCAGGTGAAC 
AACAGCAACA ACAAGAAGGG TACCTTCACG GACGACCTGC ACAAGCTGGT GGACGAGTGG 
ACGAGCAAGA CGGTGGGGGC CGCGCAGCTG AAGCCCACGC TCAACCAGCT GAAGCAGACC 
CAGAAGCTGC AAGACATGGA GGCCCAGGCA GGCTGGGCTG CCCCTGGCGA GGCGCGGGCT 
TCTGTGCGGT TGACTCCTTT TCCTCCCCGC CTGGAGATCC CCGTGGTGTC GACTGGAAGC 
ATGGAGGCAC CTTGGGGAGA TGACCGCACC TCGAGCAGGA GTGGGGATGC CACGTCTGCC 
CCCAGCGCCC GGCCCTCTGT CCACCACGGT CATTCCCGGA GCCGCCCCGA CCCTGTCCGT 
GCCCACACCA GATGGCGCCC TCGGAACCGC CCGGAGAAAC CAGGTGTGGT TTGGCCTCCG 
AGTCCCCCCC ACCGCCTGCT GTGGGCACAG CACTCAGCCG CGAGGGGGAC AGCGGGTGGG 
CAGCAAGACT GCTTCCTTTG CGGCTTCAGA CCCTGTTCGC TCCTAGGTTC CTGTGGTCCA 
CGCGCCGTCT CCACACCCAC TTCCTATACT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517238.1
CDS	233..6805
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518936.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518936.2

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGGTCCTC CACCAGCAGC 
CTGGCCCCAG GCCCTGAGCC AGGCCCCCAG CCCGCCCTGC ACGTCCAGGC GCAGGTGAAC 
AACAGCAACA ACAAGAAGGG TACCTTCACG GACGACCTGC ACAAGCTGGT GGACGAGTGG 
ACGAGCAAGA CGGTGGGGGC CGCGCAGCTG AAGCCCACGC TCAACCAGCT GAAGCAGACC 
CAGAAGCTGC AAGACATGGA GGCCCAGGCA GGCTGGGCTG CCCCTGGCGA GGCGCGGGCT 
TCTGTGCGGT TGACTCCTTT TCCTCCCCGC CTGGAGATCC CCGTGGTGTC GACTGGAAGC 
ATGGAGGCAC CTTGGGGAGA TGACCGCACC TCGAGCAGGA GTGGGGATGC CACGTCTGCC 
CCCAGCGCCC GGCCCTCTGT CCACCACGGT CATTCCCGGA GCCGCCCCGA CCCTGTCCGT 
GCCCACACCA GATGGCGCCC TCGGAACCGC CCGGAGAAAC CAGGTGTGGT TTGGCCTCCG 
AGTCCCCCCC ACCGCCTGCT GTGGGCACAG CACTCAGCCG CGAGGGGGAC AGCGGGTGGG 
CAGCAAGACT GCTTCCTTTG CGGCTTCAGA CCCTGTTCGC TCCTAGGTTC CTGTGGTCCA 
CGCGCCGTCT CCACACCCAC TTCCTATACT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518937.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517239.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X26, mRNA.

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CloneID	OHu71658
Clone ID Related Accession (Same CDS sequence)	XM_011518937.2 , XM_011518937.3
Accession Version	XM_011518937.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACCTG GGACATACGT AGTTCCTGGC CTCCAGGACC GGAAGCTCAC CAAGCTGGAG 
CGGCAGCGGT TCAAGGAAGA GGCTGAGATG CTGAAAGGCC TGCAGCACCC CAACATCGTG 
CGCTTCTACG ACTTCTGGGA GTCCAGCGCC AAGGGCAAGC GGTGCATTGT GCTGGTGACG 
GAGCTGATGA CCTCAGGGAC GCTGAAGACA TACCTGAAGC GGTTCAAGGT GATGAAGCCC 
AAGGTTCTCC GCAGCTGGTG CCGGCAGATC CTGAAGGGCC TGCTGTTCCT GCACACAAGG 
ACGCCACCCA TCATCCACCG AGACCTGAAA TGTGACAATA TTTTCATCAC CGGACCAACT 
GGGTCTGTGA AGATTGGCGA CTTGGGCCTG GCCACTCTGA AAAGAGCGTC ATTTGCCAAA 
AGTGTGATAG GTACTCCCGA GTTCATGGCG CCCGAGATGT ACGAGGAGCA CTACGATGAG 
TCCGTGGACG TCTATGCCTT TGGGATGTGC ATGCTGGAGA TGGCCACCTC GGAGTACCCC 
TACTCGGAGT GCCAGAATGC GGCCCAGATC TACCGCAAGG TCACCTGTGG TATCAAGCCG 
GCCAGCTTTG AGAAAGTGCA CGATCCTGAA ATCAAGGAGA TTATTGGGGA GTGTATCTGC 
AAAAACAAGG AGGAAAGGTA CGAGATCAAA GACCTGCTGA GCCACGCCTT CTTCGCAGAG 
GACACAGGCG TGAGGGTGGA GCTCGCGGAG GAGGACCACG GCAGGAAGTC CACCATCGCC 
CTGAGGCTCT GGGTGGAAGA CCCCAAGAAA CTGAAGGGAA AGCCCAAGGA CAATGGAGCC 
ATAGAGTTCA CCTTCGACCT GGAGAAGGAG ACGCCGGATG AGGTGGCCCA AGAGATGATT 
GAGTCTGGAT TCTTCCACGA GAGTGACGTC AAGATCGTGG CCAAGTCCAT CCGTGACCGC 
GTGGCCTTGA TCCAGTGGCG GCGGGAGAGG ATCTGGCCCG CGCTGCAGCC CAAGGAGCAG 
CAGGATGTGG GCAGCCCGGA CAAGGCCAGG GGTCCGCCGG TGCCCCTGCA GGTCCAGGTG 
ACCTACCATG CACAGGCTGG GCAGCCCGGG CCACCAGAGC CCGAGGAGCC GGAGGCCGAC 
CAGCACCTCC TGCCACCTAC GTTGCCGACC AGCGCCACCT CCCTGGCCTC GGACAGCACC 
TTCGACAGCG GCCAGGGCTC TACCGTGTAC TCAGACTCGC AGAGCAGCCA GCAGAGCGTG 
ATGCTTGGCT CCCTTGCCGA CGCAGCGCCG TCCCCGGCCC AGTGTGTGTG CAGCCCCCCT 
GTGAGCGAGG GGCCCGTCCT GCCGCAGAGC CTGCCCTCGC TGGGGGCCTA CCAGCAGCCC 
ACGGCTGCAC CTGGCTTGCC GGTGGGCTCT GTCCCGGCCC CCGCCTGCCC TCCGTCCCTC 
CAGCAGCACT TCCCGGATCC GGCCATGAGC TTCGCCCCCG TGCTGCCGCC GCCCAGCACC 
CCCATGCCCA CGGGCCCAGG CCAGCCAGCA CCCCCCGGCC AGCAGCCTCC TCCGCTGGCC 
CAGCCGACAC CCCTGCCGCA GGTCCTGGCC CCACAGCCCG TGGTCCCCCT CCAGCCGGTT 
CCCCCCCACC TGCCACCGTA CCTGGCTCCA GCCTCCCAGG TGGGGGCCCC CGCTCAGCTG 
AAGCCCCTCC AGATGCCACA GGCGCCCCTG CAGCCGCTTG CTCAAGTCCC TCCGCAGATG 
CCCCCGATTC CTGTTGTGCC CCCCATCACG CCCCTGGCGG GAATCGACGG CCTCCCTCCG 
GCCCTCCCAG ACCTGCCGAC CGCGACTGTG CCTCCCGTGC CACCACCTCA GTATTTCTCT 
CCAGCCGTGA TCTTGCCGAG CCTCGCTGCC CCACTCCCCC CTGCGTCCCC AGCCTTGCCT 
CTGCAGGCTG TGAAGCTGCC CCACCCCCCT GGGGCGCCCC TGGCCATGCC CTGCCGGACC 
ATTGTGCCAA ATGCACCGGC CACTATCCCC CTGCTGGCCG TAGCCCCACC GGGCGTGGCT 
GCCCTGTCCA TTCATTCTGC CGTGGCCCAG CTCCCAGGCC AACCTGTGTA CCCAGCGGCC 
TTCCCACAGA TGGCGCCTAC TGACGTCCCT CCTTCCCCCC ATCACACGGT GCAGAATATG 
AGGGCCACCC CTCCACAGCC GGCACTGCCT CCACAACCCA CACTGCCCCC ACAACCCGTG 
CTGCCCCCGC AACCCACGCT GCCCCCTCAA CCTGTGTTGC CCCCGCAACC CACACGGCCC 
CCTCAACCTG TGCTGCCCCC GCAACCCATG CTGCCCCCAC AACCTGTGCT GCCCCCGCAG 
CCGGCACTGC CTGTGCGCCC TGAGCCCCTC CAGCCCCACC TTCCTGAACA AGCTGCTCCA 
GCTGCTACAC CAGGGAGCCA GATTCTGCTT GGCCACCCAG CTCCCTATGC TGTGGACGTC 
GCCGCTCAGG TCCCCACCGT GCCTGTGCCA CCGGCTGCGG TCCTCTCGCC GCCTCTGCCG 
GAAGTGCTGC TGCCTGCCGC CCCTGAGCTC CTGCCTCAGT TCCCCAGCTC CCTGGCCACG 
GTGTCTGCCT CTGTGCAGAG TGTGCCCACC CAGACTGCCA CACTTCTGCC ACCAGCAAAC 
CCACCGCTGC CTGGCGGGCC CGGGATCGCC AGCCCTTGCC CAACTGTCCA GCTGACGGTG 
GAACCAGTCC AAGAGGAGCA GGCCTCACAG GACAAGCCGC CCGGCCTCCC GCAGAGCTGT 
GAGAGCTATG GAGGTTCTGA TGTCACTTCT GGAAAAGAGC TGAGTGACAG CTGTGAAGGC 
GCCTTTGGAG GGGGCAGGCT GGAGGGCAGG GCAGCCCGAA AACACCACCG CAGGTCCACG 
CGTGCGCGCT CCCGGCAGGA GAGGGCCAGC CGGCCCCGGC TTACCATCTT GAACGTGTGC 
AACACTGGGG ACAAGATGGT GGAGTGCCAG CTGGAGACGC ACAACCACAA GATGGTGACC 
TTCAAGTTCG ACTTGGACGG GGACGCACCC GATGAAATTG CCACGTATAT GGTGGAGCAT 
GACTTTATCC TGCAGGCCGA GCGGGAAACG TTCATCGAGC AGATGAAGGA TGTCATGGAC 
AAGGCAGAGG ACATGCTCAG CGAGGACACA GACGCCGACC GTGGCTCCGA CCCAGGGACC 
AGCCCGCCAC ACCTCAGCAC CTGCGGCCTG GGCACCGGGG AGGAGAGCCG ACAATCCCAA 
GCCAACGCCC CCGTGTATCA GCAGAACGTC CTGCACACCG GGAAGAGGTG GTTCATCATC 
TGTCCGGTGG CTGAGCACCC CGCCCCCGAG GCCCCTGAAT CTTCGCCCCC ACTTCCTCTA 
AGCTCCCTGC CGCCAGAAGC CAGCCAAGAT TCAGCGCCCT ATAAAGACCA GCTGTCCTCG 
AAGGAACAAC CCAGCTTTCT AGCCAGTCAG CAGCTCCTGA GCCAGGCGGG CCCCAGCAAC 
CCTCCTGGGG CACCCCCAGC CCCTTTGGCC CCCTCCTCCC CTCCTGTGAC TGCTCTGCCC 
CAAGATGGAG CAGCTCCAGC CACCAGCACC ATGCCAGAGC CAGCGTCAGG AACTGCCAGC 
CAGGCAGGGG GTCCAGGGAC ACCTCAGGGG CTGACCAGTG AGCTCGAGAC GTCTCAGCCA 
CTAGCGGAGA CTCACGAGGC CCCGCTTGCT GTGCAGCCCC TCGTGGTGGG CCTAGCACCT 
TGCACTCCAG CTCCAGAGGC TGCCTCAACC AGGGACGCCA GTGCCCCAAG GGAGCCCCTG 
CCACCTCCTG CACCTGAGCC CAGCCCCCAC AGCGGGACCC CACAGCCCGC CTTGGGTCAA 
CCTGCTCCCC TGCTTCCTGC CGCAGTGGGG GCCGTCAGCC TGGCCACCTC CCAGCTCCCA 
AGCCCACCCC TGGGGCCCAC CGTCCCCCCA CAGCCACCCT CGGCCCTGGA GTCGGATGGG 
GAAGGGCCGC CCCCCAGGGT GGGCTTTGTG GACAGCACCA TCAAGAGCCT GGACGAGAAG 
CTGCGGACTC TGCTCTACCA GGAGCACGTG CCCACCTCCT CAGCCTCAGC TGGGACCCCT 
GTGGAGGTGG GCGACAGAGA CTTCACCCTG GAGCCCCTGA GAGGGGACCA GCCCCGCTCA 
GAGGTCTGCG GGGGGGACCT GGCCCTGCCC CCAGTGCCTA AGGAGGCGGT CTCAGGGCGT 
GTCCAGCTGC CCCAGCCCTT GGTGGAGAAG TCAGAACTGG CCCCCACTCG AGGGGCCGTG 
ATGGAGCAGG GCACGTCCTC GTCAATGACA GCAGAGTCGT CTCCCAGGAG TATGCTAGGC 
TATGACAGAG ATGGAAGGCA GGTGGCCTCA GACTCCCATG TGGTCCCCAG CGTCCCCCAG 
GATGTACCTG CTTTTGTGAG ACCTGCACGT GTGGAGCCCA CAGACAGGGA TGGTGGAGAA 
GCTGGAGAAA GCTCGGCAGA GCCCCCGCCG AGTGACATGG GCACAGTGGG GGGCCAGGCT 
AGCCACCCCC AGACACTCGG CGCTCGAGCT TTGGGGTCCC CTCGGAAACG TCCAGAGCAG 
CAGGATGTCA GCTCACCAGC CAAGACTGTG GGCCGTTTCT CGGTGGTCAG CACTCAGGAC 
GAGTGGACCC TGGCCTCCCC CCACAGCCTG AGATACTCTG CCCCACCCGA CGTCTACCTG 
GACGAGGCCC CCTCCAGCCC CGACGTGAAG CTGGCAGTGC GGCGGGCGCA GACGGCCTCC 
TCCATCGAGG TCGGCGTGGG CGAGCCCGTG TCCAGCGACT CTGGGGACGA GGGCCCTCGG 
GCGAGACCCC CGGTGCAGAA GCAGGCGTCC CTGCCCGTGA GTGGCAGCGT GGCTGGCGAC 
TTCGTGAAGA AGGCCACCGC CTTCCTGCAG AGGCCTTCTC GGGCCGGCTC GCTGGGCCCC 
GAGACACCCA GCAGGGTGGG CATGAAGGTC CCCACGATCA GCGTGACCTC CTTCCATTCC 
CAGTCGTCCT ACATCAGCAG CGACAATGAT TCGGAGCTCG AGGATGCTGA CATAAAGAAG 
GAGCTGCAGA GTCTGCGGGA GAAGCACCTG AAGGAGATCT CGGAGCTGCA GAGCCAGCAG 
AAGCAGGAGA TCGAAGCTCT GTACCGCCGC CTGGGCAAGC CACTGCCCCC CAACGTGGGC 
TTCTTCCACA CGGCACCCCC CACTGGCCGC CGGAGAAAAA CCAGCAAGAG CAAGCTGAAG 
GCAGGCAAGC TGCTAAATCC CCTGGTGCGG CAGCTCAAGG TCGTGGCCTC CAGCACAGGT 
CACTTGGCTG ACTCCAGCAG AGGCCCTCCC GCTAAGGACC CTGCCCAAGC CAGTGTGGGG 
CTCACTGCAG ACAGCACGGG CCTGAGCGGG AAGGCAGTGC AGACCCAGCA GCCCTGCTCC 
GTCCGGGCCT CCCTGTCTTC GGACATCTGC TCCGGCTTAG CCAGTGATGG AGGCGGAGCG 
CGTGGCCAAG GCTGGACGGT TTACCACCCA ACGTCTGAGA GAGTGACCTA TAAGTCTAGT 
AGCAAACCTC GTGCTCGATT CCTCAGTGGA CCCGTATCTG TGTCCATCTG GTCTGCCCTG 
AAGCGTCTCT GCCTAGGCAA AGAACACAGC AGTAGGTCCT CCACCAGCAG CCTGGCCCCA 
GGCCCTGAGC CAGGCCCCCA GCCCGCCCTG CACGTCCAGG CGCAGGTGAA CAACAGCAAC 
AACAAGAAGG GTACCTTCAC GGACGACCTG CACAAGCTGG TGGACGAGTG GACGAGCAAG 
ACGGTGGGGG CCGCGCAGCT GAAGCCCACG CTCAACCAGC TGAAGCAGAC CCAGAAGCTG 
CAAGACATGG AGGCCCAGGC AGGCTGGGCT GCCCCTGGCG AGGCGCGGGC TTCTGTGCGG 
TTGACTCCTT TTCCTCCCCG CCTGGAGATC CCCGTGGTGT CGACTGGAAG CATGGAGGCA 
CCTTGGGGAG ATGACCGCAC CTCGAGCAGG AGTGGGGATG CCACGTCTGC CCCCAGCGCC 
CGGCCCTCTG TCCACCACGG TCATTCCCGG AGCCGCCCCG ACCCTGTCCG TGCCCACACC 
AGATGGCGCC CTCGGAACCG CCCGGAGAAA CCAGGTGTGG TTTGGCCTCC GAGTCCCCCC 
CACCGCCTGC TGTGGGCACA GCACTCAGCC GCGAGGGGGA CAGCGGGTGG GCAGCAAGAC 
TGCTTCCTTT GCGGCTTCAG ACCCTGTTCG CTCCTAGGTT CCTGTGGTCC ACGCGCCGTC 
TCCACACCCA CTTCCTATAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517239.1
CDS	186..6449
Translation	MLPGTYVVPGLQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518937.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518937.2

ORF Insert Sequence:

ATGCTACCTG GGACATACGT AGTTCCTGGC CTCCAGGACC GGAAGCTCAC CAAGCTGGAG 
CGGCAGCGGT TCAAGGAAGA GGCTGAGATG CTGAAAGGCC TGCAGCACCC CAACATCGTG 
CGCTTCTACG ACTTCTGGGA GTCCAGCGCC AAGGGCAAGC GGTGCATTGT GCTGGTGACG 
GAGCTGATGA CCTCAGGGAC GCTGAAGACA TACCTGAAGC GGTTCAAGGT GATGAAGCCC 
AAGGTTCTCC GCAGCTGGTG CCGGCAGATC CTGAAGGGCC TGCTGTTCCT GCACACAAGG 
ACGCCACCCA TCATCCACCG AGACCTGAAA TGTGACAATA TTTTCATCAC CGGACCAACT 
GGGTCTGTGA AGATTGGCGA CTTGGGCCTG GCCACTCTGA AAAGAGCGTC ATTTGCCAAA 
AGTGTGATAG GTACTCCCGA GTTCATGGCG CCCGAGATGT ACGAGGAGCA CTACGATGAG 
TCCGTGGACG TCTATGCCTT TGGGATGTGC ATGCTGGAGA TGGCCACCTC GGAGTACCCC 
TACTCGGAGT GCCAGAATGC GGCCCAGATC TACCGCAAGG TCACCTGTGG TATCAAGCCG 
GCCAGCTTTG AGAAAGTGCA CGATCCTGAA ATCAAGGAGA TTATTGGGGA GTGTATCTGC 
AAAAACAAGG AGGAAAGGTA CGAGATCAAA GACCTGCTGA GCCACGCCTT CTTCGCAGAG 
GACACAGGCG TGAGGGTGGA GCTCGCGGAG GAGGACCACG GCAGGAAGTC CACCATCGCC 
CTGAGGCTCT GGGTGGAAGA CCCCAAGAAA CTGAAGGGAA AGCCCAAGGA CAATGGAGCC 
ATAGAGTTCA CCTTCGACCT GGAGAAGGAG ACGCCGGATG AGGTGGCCCA AGAGATGATT 
GAGTCTGGAT TCTTCCACGA GAGTGACGTC AAGATCGTGG CCAAGTCCAT CCGTGACCGC 
GTGGCCTTGA TCCAGTGGCG GCGGGAGAGG ATCTGGCCCG CGCTGCAGCC CAAGGAGCAG 
CAGGATGTGG GCAGCCCGGA CAAGGCCAGG GGTCCGCCGG TGCCCCTGCA GGTCCAGGTG 
ACCTACCATG CACAGGCTGG GCAGCCCGGG CCACCAGAGC CCGAGGAGCC GGAGGCCGAC 
CAGCACCTCC TGCCACCTAC GTTGCCGACC AGCGCCACCT CCCTGGCCTC GGACAGCACC 
TTCGACAGCG GCCAGGGCTC TACCGTGTAC TCAGACTCGC AGAGCAGCCA GCAGAGCGTG 
ATGCTTGGCT CCCTTGCCGA CGCAGCGCCG TCCCCGGCCC AGTGTGTGTG CAGCCCCCCT 
GTGAGCGAGG GGCCCGTCCT GCCGCAGAGC CTGCCCTCGC TGGGGGCCTA CCAGCAGCCC 
ACGGCTGCAC CTGGCTTGCC GGTGGGCTCT GTCCCGGCCC CCGCCTGCCC TCCGTCCCTC 
CAGCAGCACT TCCCGGATCC GGCCATGAGC TTCGCCCCCG TGCTGCCGCC GCCCAGCACC 
CCCATGCCCA CGGGCCCAGG CCAGCCAGCA CCCCCCGGCC AGCAGCCTCC TCCGCTGGCC 
CAGCCGACAC CCCTGCCGCA GGTCCTGGCC CCACAGCCCG TGGTCCCCCT CCAGCCGGTT 
CCCCCCCACC TGCCACCGTA CCTGGCTCCA GCCTCCCAGG TGGGGGCCCC CGCTCAGCTG 
AAGCCCCTCC AGATGCCACA GGCGCCCCTG CAGCCGCTTG CTCAAGTCCC TCCGCAGATG 
CCCCCGATTC CTGTTGTGCC CCCCATCACG CCCCTGGCGG GAATCGACGG CCTCCCTCCG 
GCCCTCCCAG ACCTGCCGAC CGCGACTGTG CCTCCCGTGC CACCACCTCA GTATTTCTCT 
CCAGCCGTGA TCTTGCCGAG CCTCGCTGCC CCACTCCCCC CTGCGTCCCC AGCCTTGCCT 
CTGCAGGCTG TGAAGCTGCC CCACCCCCCT GGGGCGCCCC TGGCCATGCC CTGCCGGACC 
ATTGTGCCAA ATGCACCGGC CACTATCCCC CTGCTGGCCG TAGCCCCACC GGGCGTGGCT 
GCCCTGTCCA TTCATTCTGC CGTGGCCCAG CTCCCAGGCC AACCTGTGTA CCCAGCGGCC 
TTCCCACAGA TGGCGCCTAC TGACGTCCCT CCTTCCCCCC ATCACACGGT GCAGAATATG 
AGGGCCACCC CTCCACAGCC GGCACTGCCT CCACAACCCA CACTGCCCCC ACAACCCGTG 
CTGCCCCCGC AACCCACGCT GCCCCCTCAA CCTGTGTTGC CCCCGCAACC CACACGGCCC 
CCTCAACCTG TGCTGCCCCC GCAACCCATG CTGCCCCCAC AACCTGTGCT GCCCCCGCAG 
CCGGCACTGC CTGTGCGCCC TGAGCCCCTC CAGCCCCACC TTCCTGAACA AGCTGCTCCA 
GCTGCTACAC CAGGGAGCCA GATTCTGCTT GGCCACCCAG CTCCCTATGC TGTGGACGTC 
GCCGCTCAGG TCCCCACCGT GCCTGTGCCA CCGGCTGCGG TCCTCTCGCC GCCTCTGCCG 
GAAGTGCTGC TGCCTGCCGC CCCTGAGCTC CTGCCTCAGT TCCCCAGCTC CCTGGCCACG 
GTGTCTGCCT CTGTGCAGAG TGTGCCCACC CAGACTGCCA CACTTCTGCC ACCAGCAAAC 
CCACCGCTGC CTGGCGGGCC CGGGATCGCC AGCCCTTGCC CAACTGTCCA GCTGACGGTG 
GAACCAGTCC AAGAGGAGCA GGCCTCACAG GACAAGCCGC CCGGCCTCCC GCAGAGCTGT 
GAGAGCTATG GAGGTTCTGA TGTCACTTCT GGAAAAGAGC TGAGTGACAG CTGTGAAGGC 
GCCTTTGGAG GGGGCAGGCT GGAGGGCAGG GCAGCCCGAA AACACCACCG CAGGTCCACG 
CGTGCGCGCT CCCGGCAGGA GAGGGCCAGC CGGCCCCGGC TTACCATCTT GAACGTGTGC 
AACACTGGGG ACAAGATGGT GGAGTGCCAG CTGGAGACGC ACAACCACAA GATGGTGACC 
TTCAAGTTCG ACTTGGACGG GGACGCACCC GATGAAATTG CCACGTATAT GGTGGAGCAT 
GACTTTATCC TGCAGGCCGA GCGGGAAACG TTCATCGAGC AGATGAAGGA TGTCATGGAC 
AAGGCAGAGG ACATGCTCAG CGAGGACACA GACGCCGACC GTGGCTCCGA CCCAGGGACC 
AGCCCGCCAC ACCTCAGCAC CTGCGGCCTG GGCACCGGGG AGGAGAGCCG ACAATCCCAA 
GCCAACGCCC CCGTGTATCA GCAGAACGTC CTGCACACCG GGAAGAGGTG GTTCATCATC 
TGTCCGGTGG CTGAGCACCC CGCCCCCGAG GCCCCTGAAT CTTCGCCCCC ACTTCCTCTA 
AGCTCCCTGC CGCCAGAAGC CAGCCAAGAT TCAGCGCCCT ATAAAGACCA GCTGTCCTCG 
AAGGAACAAC CCAGCTTTCT AGCCAGTCAG CAGCTCCTGA GCCAGGCGGG CCCCAGCAAC 
CCTCCTGGGG CACCCCCAGC CCCTTTGGCC CCCTCCTCCC CTCCTGTGAC TGCTCTGCCC 
CAAGATGGAG CAGCTCCAGC CACCAGCACC ATGCCAGAGC CAGCGTCAGG AACTGCCAGC 
CAGGCAGGGG GTCCAGGGAC ACCTCAGGGG CTGACCAGTG AGCTCGAGAC GTCTCAGCCA 
CTAGCGGAGA CTCACGAGGC CCCGCTTGCT GTGCAGCCCC TCGTGGTGGG CCTAGCACCT 
TGCACTCCAG CTCCAGAGGC TGCCTCAACC AGGGACGCCA GTGCCCCAAG GGAGCCCCTG 
CCACCTCCTG CACCTGAGCC CAGCCCCCAC AGCGGGACCC CACAGCCCGC CTTGGGTCAA 
CCTGCTCCCC TGCTTCCTGC CGCAGTGGGG GCCGTCAGCC TGGCCACCTC CCAGCTCCCA 
AGCCCACCCC TGGGGCCCAC CGTCCCCCCA CAGCCACCCT CGGCCCTGGA GTCGGATGGG 
GAAGGGCCGC CCCCCAGGGT GGGCTTTGTG GACAGCACCA TCAAGAGCCT GGACGAGAAG 
CTGCGGACTC TGCTCTACCA GGAGCACGTG CCCACCTCCT CAGCCTCAGC TGGGACCCCT 
GTGGAGGTGG GCGACAGAGA CTTCACCCTG GAGCCCCTGA GAGGGGACCA GCCCCGCTCA 
GAGGTCTGCG GGGGGGACCT GGCCCTGCCC CCAGTGCCTA AGGAGGCGGT CTCAGGGCGT 
GTCCAGCTGC CCCAGCCCTT GGTGGAGAAG TCAGAACTGG CCCCCACTCG AGGGGCCGTG 
ATGGAGCAGG GCACGTCCTC GTCAATGACA GCAGAGTCGT CTCCCAGGAG TATGCTAGGC 
TATGACAGAG ATGGAAGGCA GGTGGCCTCA GACTCCCATG TGGTCCCCAG CGTCCCCCAG 
GATGTACCTG CTTTTGTGAG ACCTGCACGT GTGGAGCCCA CAGACAGGGA TGGTGGAGAA 
GCTGGAGAAA GCTCGGCAGA GCCCCCGCCG AGTGACATGG GCACAGTGGG GGGCCAGGCT 
AGCCACCCCC AGACACTCGG CGCTCGAGCT TTGGGGTCCC CTCGGAAACG TCCAGAGCAG 
CAGGATGTCA GCTCACCAGC CAAGACTGTG GGCCGTTTCT CGGTGGTCAG CACTCAGGAC 
GAGTGGACCC TGGCCTCCCC CCACAGCCTG AGATACTCTG CCCCACCCGA CGTCTACCTG 
GACGAGGCCC CCTCCAGCCC CGACGTGAAG CTGGCAGTGC GGCGGGCGCA GACGGCCTCC 
TCCATCGAGG TCGGCGTGGG CGAGCCCGTG TCCAGCGACT CTGGGGACGA GGGCCCTCGG 
GCGAGACCCC CGGTGCAGAA GCAGGCGTCC CTGCCCGTGA GTGGCAGCGT GGCTGGCGAC 
TTCGTGAAGA AGGCCACCGC CTTCCTGCAG AGGCCTTCTC GGGCCGGCTC GCTGGGCCCC 
GAGACACCCA GCAGGGTGGG CATGAAGGTC CCCACGATCA GCGTGACCTC CTTCCATTCC 
CAGTCGTCCT ACATCAGCAG CGACAATGAT TCGGAGCTCG AGGATGCTGA CATAAAGAAG 
GAGCTGCAGA GTCTGCGGGA GAAGCACCTG AAGGAGATCT CGGAGCTGCA GAGCCAGCAG 
AAGCAGGAGA TCGAAGCTCT GTACCGCCGC CTGGGCAAGC CACTGCCCCC CAACGTGGGC 
TTCTTCCACA CGGCACCCCC CACTGGCCGC CGGAGAAAAA CCAGCAAGAG CAAGCTGAAG 
GCAGGCAAGC TGCTAAATCC CCTGGTGCGG CAGCTCAAGG TCGTGGCCTC CAGCACAGGT 
CACTTGGCTG ACTCCAGCAG AGGCCCTCCC GCTAAGGACC CTGCCCAAGC CAGTGTGGGG 
CTCACTGCAG ACAGCACGGG CCTGAGCGGG AAGGCAGTGC AGACCCAGCA GCCCTGCTCC 
GTCCGGGCCT CCCTGTCTTC GGACATCTGC TCCGGCTTAG CCAGTGATGG AGGCGGAGCG 
CGTGGCCAAG GCTGGACGGT TTACCACCCA ACGTCTGAGA GAGTGACCTA TAAGTCTAGT 
AGCAAACCTC GTGCTCGATT CCTCAGTGGA CCCGTATCTG TGTCCATCTG GTCTGCCCTG 
AAGCGTCTCT GCCTAGGCAA AGAACACAGC AGTAGGTCCT CCACCAGCAG CCTGGCCCCA 
GGCCCTGAGC CAGGCCCCCA GCCCGCCCTG CACGTCCAGG CGCAGGTGAA CAACAGCAAC 
AACAAGAAGG GTACCTTCAC GGACGACCTG CACAAGCTGG TGGACGAGTG GACGAGCAAG 
ACGGTGGGGG CCGCGCAGCT GAAGCCCACG CTCAACCAGC TGAAGCAGAC CCAGAAGCTG 
CAAGACATGG AGGCCCAGGC AGGCTGGGCT GCCCCTGGCG AGGCGCGGGC TTCTGTGCGG 
TTGACTCCTT TTCCTCCCCG CCTGGAGATC CCCGTGGTGT CGACTGGAAG CATGGAGGCA 
CCTTGGGGAG ATGACCGCAC CTCGAGCAGG AGTGGGGATG CCACGTCTGC CCCCAGCGCC 
CGGCCCTCTG TCCACCACGG TCATTCCCGG AGCCGCCCCG ACCCTGTCCG TGCCCACACC 
AGATGGCGCC CTCGGAACCG CCCGGAGAAA CCAGGTGTGG TTTGGCCTCC GAGTCCCCCC 
CACCGCCTGC TGTGGGCACA GCACTCAGCC GCGAGGGGGA CAGCGGGTGG GCAGCAAGAC 
TGCTTCCTTT GCGGCTTCAG ACCCTGTTCG CTCCTAGGTT CCTGTGGTCC ACGCGCCGTC 
TCCACACCCA CTTCCTATAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518937.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517239.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X28, mRNA.

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CloneID	OHu71658
Clone ID Related Accession (Same CDS sequence)	XM_011518937.2 , XM_011518937.3
Accession Version	XM_011518937.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACCTG GGACATACGT AGTTCCTGGC CTCCAGGACC GGAAGCTCAC CAAGCTGGAG 
CGGCAGCGGT TCAAGGAAGA GGCTGAGATG CTGAAAGGCC TGCAGCACCC CAACATCGTG 
CGCTTCTACG ACTTCTGGGA GTCCAGCGCC AAGGGCAAGC GGTGCATTGT GCTGGTGACG 
GAGCTGATGA CCTCAGGGAC GCTGAAGACA TACCTGAAGC GGTTCAAGGT GATGAAGCCC 
AAGGTTCTCC GCAGCTGGTG CCGGCAGATC CTGAAGGGCC TGCTGTTCCT GCACACAAGG 
ACGCCACCCA TCATCCACCG AGACCTGAAA TGTGACAATA TTTTCATCAC CGGACCAACT 
GGGTCTGTGA AGATTGGCGA CTTGGGCCTG GCCACTCTGA AAAGAGCGTC ATTTGCCAAA 
AGTGTGATAG GTACTCCCGA GTTCATGGCG CCCGAGATGT ACGAGGAGCA CTACGATGAG 
TCCGTGGACG TCTATGCCTT TGGGATGTGC ATGCTGGAGA TGGCCACCTC GGAGTACCCC 
TACTCGGAGT GCCAGAATGC GGCCCAGATC TACCGCAAGG TCACCTGTGG TATCAAGCCG 
GCCAGCTTTG AGAAAGTGCA CGATCCTGAA ATCAAGGAGA TTATTGGGGA GTGTATCTGC 
AAAAACAAGG AGGAAAGGTA CGAGATCAAA GACCTGCTGA GCCACGCCTT CTTCGCAGAG 
GACACAGGCG TGAGGGTGGA GCTCGCGGAG GAGGACCACG GCAGGAAGTC CACCATCGCC 
CTGAGGCTCT GGGTGGAAGA CCCCAAGAAA CTGAAGGGAA AGCCCAAGGA CAATGGAGCC 
ATAGAGTTCA CCTTCGACCT GGAGAAGGAG ACGCCGGATG AGGTGGCCCA AGAGATGATT 
GAGTCTGGAT TCTTCCACGA GAGTGACGTC AAGATCGTGG CCAAGTCCAT CCGTGACCGC 
GTGGCCTTGA TCCAGTGGCG GCGGGAGAGG ATCTGGCCCG CGCTGCAGCC CAAGGAGCAG 
CAGGATGTGG GCAGCCCGGA CAAGGCCAGG GGTCCGCCGG TGCCCCTGCA GGTCCAGGTG 
ACCTACCATG CACAGGCTGG GCAGCCCGGG CCACCAGAGC CCGAGGAGCC GGAGGCCGAC 
CAGCACCTCC TGCCACCTAC GTTGCCGACC AGCGCCACCT CCCTGGCCTC GGACAGCACC 
TTCGACAGCG GCCAGGGCTC TACCGTGTAC TCAGACTCGC AGAGCAGCCA GCAGAGCGTG 
ATGCTTGGCT CCCTTGCCGA CGCAGCGCCG TCCCCGGCCC AGTGTGTGTG CAGCCCCCCT 
GTGAGCGAGG GGCCCGTCCT GCCGCAGAGC CTGCCCTCGC TGGGGGCCTA CCAGCAGCCC 
ACGGCTGCAC CTGGCTTGCC GGTGGGCTCT GTCCCGGCCC CCGCCTGCCC TCCGTCCCTC 
CAGCAGCACT TCCCGGATCC GGCCATGAGC TTCGCCCCCG TGCTGCCGCC GCCCAGCACC 
CCCATGCCCA CGGGCCCAGG CCAGCCAGCA CCCCCCGGCC AGCAGCCTCC TCCGCTGGCC 
CAGCCGACAC CCCTGCCGCA GGTCCTGGCC CCACAGCCCG TGGTCCCCCT CCAGCCGGTT 
CCCCCCCACC TGCCACCGTA CCTGGCTCCA GCCTCCCAGG TGGGGGCCCC CGCTCAGCTG 
AAGCCCCTCC AGATGCCACA GGCGCCCCTG CAGCCGCTTG CTCAAGTCCC TCCGCAGATG 
CCCCCGATTC CTGTTGTGCC CCCCATCACG CCCCTGGCGG GAATCGACGG CCTCCCTCCG 
GCCCTCCCAG ACCTGCCGAC CGCGACTGTG CCTCCCGTGC CACCACCTCA GTATTTCTCT 
CCAGCCGTGA TCTTGCCGAG CCTCGCTGCC CCACTCCCCC CTGCGTCCCC AGCCTTGCCT 
CTGCAGGCTG TGAAGCTGCC CCACCCCCCT GGGGCGCCCC TGGCCATGCC CTGCCGGACC 
ATTGTGCCAA ATGCACCGGC CACTATCCCC CTGCTGGCCG TAGCCCCACC GGGCGTGGCT 
GCCCTGTCCA TTCATTCTGC CGTGGCCCAG CTCCCAGGCC AACCTGTGTA CCCAGCGGCC 
TTCCCACAGA TGGCGCCTAC TGACGTCCCT CCTTCCCCCC ATCACACGGT GCAGAATATG 
AGGGCCACCC CTCCACAGCC GGCACTGCCT CCACAACCCA CACTGCCCCC ACAACCCGTG 
CTGCCCCCGC AACCCACGCT GCCCCCTCAA CCTGTGTTGC CCCCGCAACC CACACGGCCC 
CCTCAACCTG TGCTGCCCCC GCAACCCATG CTGCCCCCAC AACCTGTGCT GCCCCCGCAG 
CCGGCACTGC CTGTGCGCCC TGAGCCCCTC CAGCCCCACC TTCCTGAACA AGCTGCTCCA 
GCTGCTACAC CAGGGAGCCA GATTCTGCTT GGCCACCCAG CTCCCTATGC TGTGGACGTC 
GCCGCTCAGG TCCCCACCGT GCCTGTGCCA CCGGCTGCGG TCCTCTCGCC GCCTCTGCCG 
GAAGTGCTGC TGCCTGCCGC CCCTGAGCTC CTGCCTCAGT TCCCCAGCTC CCTGGCCACG 
GTGTCTGCCT CTGTGCAGAG TGTGCCCACC CAGACTGCCA CACTTCTGCC ACCAGCAAAC 
CCACCGCTGC CTGGCGGGCC CGGGATCGCC AGCCCTTGCC CAACTGTCCA GCTGACGGTG 
GAACCAGTCC AAGAGGAGCA GGCCTCACAG GACAAGCCGC CCGGCCTCCC GCAGAGCTGT 
GAGAGCTATG GAGGTTCTGA TGTCACTTCT GGAAAAGAGC TGAGTGACAG CTGTGAAGGC 
GCCTTTGGAG GGGGCAGGCT GGAGGGCAGG GCAGCCCGAA AACACCACCG CAGGTCCACG 
CGTGCGCGCT CCCGGCAGGA GAGGGCCAGC CGGCCCCGGC TTACCATCTT GAACGTGTGC 
AACACTGGGG ACAAGATGGT GGAGTGCCAG CTGGAGACGC ACAACCACAA GATGGTGACC 
TTCAAGTTCG ACTTGGACGG GGACGCACCC GATGAAATTG CCACGTATAT GGTGGAGCAT 
GACTTTATCC TGCAGGCCGA GCGGGAAACG TTCATCGAGC AGATGAAGGA TGTCATGGAC 
AAGGCAGAGG ACATGCTCAG CGAGGACACA GACGCCGACC GTGGCTCCGA CCCAGGGACC 
AGCCCGCCAC ACCTCAGCAC CTGCGGCCTG GGCACCGGGG AGGAGAGCCG ACAATCCCAA 
GCCAACGCCC CCGTGTATCA GCAGAACGTC CTGCACACCG GGAAGAGGTG GTTCATCATC 
TGTCCGGTGG CTGAGCACCC CGCCCCCGAG GCCCCTGAAT CTTCGCCCCC ACTTCCTCTA 
AGCTCCCTGC CGCCAGAAGC CAGCCAAGAT TCAGCGCCCT ATAAAGACCA GCTGTCCTCG 
AAGGAACAAC CCAGCTTTCT AGCCAGTCAG CAGCTCCTGA GCCAGGCGGG CCCCAGCAAC 
CCTCCTGGGG CACCCCCAGC CCCTTTGGCC CCCTCCTCCC CTCCTGTGAC TGCTCTGCCC 
CAAGATGGAG CAGCTCCAGC CACCAGCACC ATGCCAGAGC CAGCGTCAGG AACTGCCAGC 
CAGGCAGGGG GTCCAGGGAC ACCTCAGGGG CTGACCAGTG AGCTCGAGAC GTCTCAGCCA 
CTAGCGGAGA CTCACGAGGC CCCGCTTGCT GTGCAGCCCC TCGTGGTGGG CCTAGCACCT 
TGCACTCCAG CTCCAGAGGC TGCCTCAACC AGGGACGCCA GTGCCCCAAG GGAGCCCCTG 
CCACCTCCTG CACCTGAGCC CAGCCCCCAC AGCGGGACCC CACAGCCCGC CTTGGGTCAA 
CCTGCTCCCC TGCTTCCTGC CGCAGTGGGG GCCGTCAGCC TGGCCACCTC CCAGCTCCCA 
AGCCCACCCC TGGGGCCCAC CGTCCCCCCA CAGCCACCCT CGGCCCTGGA GTCGGATGGG 
GAAGGGCCGC CCCCCAGGGT GGGCTTTGTG GACAGCACCA TCAAGAGCCT GGACGAGAAG 
CTGCGGACTC TGCTCTACCA GGAGCACGTG CCCACCTCCT CAGCCTCAGC TGGGACCCCT 
GTGGAGGTGG GCGACAGAGA CTTCACCCTG GAGCCCCTGA GAGGGGACCA GCCCCGCTCA 
GAGGTCTGCG GGGGGGACCT GGCCCTGCCC CCAGTGCCTA AGGAGGCGGT CTCAGGGCGT 
GTCCAGCTGC CCCAGCCCTT GGTGGAGAAG TCAGAACTGG CCCCCACTCG AGGGGCCGTG 
ATGGAGCAGG GCACGTCCTC GTCAATGACA GCAGAGTCGT CTCCCAGGAG TATGCTAGGC 
TATGACAGAG ATGGAAGGCA GGTGGCCTCA GACTCCCATG TGGTCCCCAG CGTCCCCCAG 
GATGTACCTG CTTTTGTGAG ACCTGCACGT GTGGAGCCCA CAGACAGGGA TGGTGGAGAA 
GCTGGAGAAA GCTCGGCAGA GCCCCCGCCG AGTGACATGG GCACAGTGGG GGGCCAGGCT 
AGCCACCCCC AGACACTCGG CGCTCGAGCT TTGGGGTCCC CTCGGAAACG TCCAGAGCAG 
CAGGATGTCA GCTCACCAGC CAAGACTGTG GGCCGTTTCT CGGTGGTCAG CACTCAGGAC 
GAGTGGACCC TGGCCTCCCC CCACAGCCTG AGATACTCTG CCCCACCCGA CGTCTACCTG 
GACGAGGCCC CCTCCAGCCC CGACGTGAAG CTGGCAGTGC GGCGGGCGCA GACGGCCTCC 
TCCATCGAGG TCGGCGTGGG CGAGCCCGTG TCCAGCGACT CTGGGGACGA GGGCCCTCGG 
GCGAGACCCC CGGTGCAGAA GCAGGCGTCC CTGCCCGTGA GTGGCAGCGT GGCTGGCGAC 
TTCGTGAAGA AGGCCACCGC CTTCCTGCAG AGGCCTTCTC GGGCCGGCTC GCTGGGCCCC 
GAGACACCCA GCAGGGTGGG CATGAAGGTC CCCACGATCA GCGTGACCTC CTTCCATTCC 
CAGTCGTCCT ACATCAGCAG CGACAATGAT TCGGAGCTCG AGGATGCTGA CATAAAGAAG 
GAGCTGCAGA GTCTGCGGGA GAAGCACCTG AAGGAGATCT CGGAGCTGCA GAGCCAGCAG 
AAGCAGGAGA TCGAAGCTCT GTACCGCCGC CTGGGCAAGC CACTGCCCCC CAACGTGGGC 
TTCTTCCACA CGGCACCCCC CACTGGCCGC CGGAGAAAAA CCAGCAAGAG CAAGCTGAAG 
GCAGGCAAGC TGCTAAATCC CCTGGTGCGG CAGCTCAAGG TCGTGGCCTC CAGCACAGGT 
CACTTGGCTG ACTCCAGCAG AGGCCCTCCC GCTAAGGACC CTGCCCAAGC CAGTGTGGGG 
CTCACTGCAG ACAGCACGGG CCTGAGCGGG AAGGCAGTGC AGACCCAGCA GCCCTGCTCC 
GTCCGGGCCT CCCTGTCTTC GGACATCTGC TCCGGCTTAG CCAGTGATGG AGGCGGAGCG 
CGTGGCCAAG GCTGGACGGT TTACCACCCA ACGTCTGAGA GAGTGACCTA TAAGTCTAGT 
AGCAAACCTC GTGCTCGATT CCTCAGTGGA CCCGTATCTG TGTCCATCTG GTCTGCCCTG 
AAGCGTCTCT GCCTAGGCAA AGAACACAGC AGTAGGTCCT CCACCAGCAG CCTGGCCCCA 
GGCCCTGAGC CAGGCCCCCA GCCCGCCCTG CACGTCCAGG CGCAGGTGAA CAACAGCAAC 
AACAAGAAGG GTACCTTCAC GGACGACCTG CACAAGCTGG TGGACGAGTG GACGAGCAAG 
ACGGTGGGGG CCGCGCAGCT GAAGCCCACG CTCAACCAGC TGAAGCAGAC CCAGAAGCTG 
CAAGACATGG AGGCCCAGGC AGGCTGGGCT GCCCCTGGCG AGGCGCGGGC TTCTGTGCGG 
TTGACTCCTT TTCCTCCCCG CCTGGAGATC CCCGTGGTGT CGACTGGAAG CATGGAGGCA 
CCTTGGGGAG ATGACCGCAC CTCGAGCAGG AGTGGGGATG CCACGTCTGC CCCCAGCGCC 
CGGCCCTCTG TCCACCACGG TCATTCCCGG AGCCGCCCCG ACCCTGTCCG TGCCCACACC 
AGATGGCGCC CTCGGAACCG CCCGGAGAAA CCAGGTGTGG TTTGGCCTCC GAGTCCCCCC 
CACCGCCTGC TGTGGGCACA GCACTCAGCC GCGAGGGGGA CAGCGGGTGG GCAGCAAGAC 
TGCTTCCTTT GCGGCTTCAG ACCCTGTTCG CTCCTAGGTT CCTGTGGTCC ACGCGCCGTC 
TCCACACCCA CTTCCTATAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517239.1
CDS	187..6450
Translation	MLPGTYVVPGLQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518937.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518937.3

ORF Insert Sequence:

ATGCTACCTG GGACATACGT AGTTCCTGGC CTCCAGGACC GGAAGCTCAC CAAGCTGGAG 
CGGCAGCGGT TCAAGGAAGA GGCTGAGATG CTGAAAGGCC TGCAGCACCC CAACATCGTG 
CGCTTCTACG ACTTCTGGGA GTCCAGCGCC AAGGGCAAGC GGTGCATTGT GCTGGTGACG 
GAGCTGATGA CCTCAGGGAC GCTGAAGACA TACCTGAAGC GGTTCAAGGT GATGAAGCCC 
AAGGTTCTCC GCAGCTGGTG CCGGCAGATC CTGAAGGGCC TGCTGTTCCT GCACACAAGG 
ACGCCACCCA TCATCCACCG AGACCTGAAA TGTGACAATA TTTTCATCAC CGGACCAACT 
GGGTCTGTGA AGATTGGCGA CTTGGGCCTG GCCACTCTGA AAAGAGCGTC ATTTGCCAAA 
AGTGTGATAG GTACTCCCGA GTTCATGGCG CCCGAGATGT ACGAGGAGCA CTACGATGAG 
TCCGTGGACG TCTATGCCTT TGGGATGTGC ATGCTGGAGA TGGCCACCTC GGAGTACCCC 
TACTCGGAGT GCCAGAATGC GGCCCAGATC TACCGCAAGG TCACCTGTGG TATCAAGCCG 
GCCAGCTTTG AGAAAGTGCA CGATCCTGAA ATCAAGGAGA TTATTGGGGA GTGTATCTGC 
AAAAACAAGG AGGAAAGGTA CGAGATCAAA GACCTGCTGA GCCACGCCTT CTTCGCAGAG 
GACACAGGCG TGAGGGTGGA GCTCGCGGAG GAGGACCACG GCAGGAAGTC CACCATCGCC 
CTGAGGCTCT GGGTGGAAGA CCCCAAGAAA CTGAAGGGAA AGCCCAAGGA CAATGGAGCC 
ATAGAGTTCA CCTTCGACCT GGAGAAGGAG ACGCCGGATG AGGTGGCCCA AGAGATGATT 
GAGTCTGGAT TCTTCCACGA GAGTGACGTC AAGATCGTGG CCAAGTCCAT CCGTGACCGC 
GTGGCCTTGA TCCAGTGGCG GCGGGAGAGG ATCTGGCCCG CGCTGCAGCC CAAGGAGCAG 
CAGGATGTGG GCAGCCCGGA CAAGGCCAGG GGTCCGCCGG TGCCCCTGCA GGTCCAGGTG 
ACCTACCATG CACAGGCTGG GCAGCCCGGG CCACCAGAGC CCGAGGAGCC GGAGGCCGAC 
CAGCACCTCC TGCCACCTAC GTTGCCGACC AGCGCCACCT CCCTGGCCTC GGACAGCACC 
TTCGACAGCG GCCAGGGCTC TACCGTGTAC TCAGACTCGC AGAGCAGCCA GCAGAGCGTG 
ATGCTTGGCT CCCTTGCCGA CGCAGCGCCG TCCCCGGCCC AGTGTGTGTG CAGCCCCCCT 
GTGAGCGAGG GGCCCGTCCT GCCGCAGAGC CTGCCCTCGC TGGGGGCCTA CCAGCAGCCC 
ACGGCTGCAC CTGGCTTGCC GGTGGGCTCT GTCCCGGCCC CCGCCTGCCC TCCGTCCCTC 
CAGCAGCACT TCCCGGATCC GGCCATGAGC TTCGCCCCCG TGCTGCCGCC GCCCAGCACC 
CCCATGCCCA CGGGCCCAGG CCAGCCAGCA CCCCCCGGCC AGCAGCCTCC TCCGCTGGCC 
CAGCCGACAC CCCTGCCGCA GGTCCTGGCC CCACAGCCCG TGGTCCCCCT CCAGCCGGTT 
CCCCCCCACC TGCCACCGTA CCTGGCTCCA GCCTCCCAGG TGGGGGCCCC CGCTCAGCTG 
AAGCCCCTCC AGATGCCACA GGCGCCCCTG CAGCCGCTTG CTCAAGTCCC TCCGCAGATG 
CCCCCGATTC CTGTTGTGCC CCCCATCACG CCCCTGGCGG GAATCGACGG CCTCCCTCCG 
GCCCTCCCAG ACCTGCCGAC CGCGACTGTG CCTCCCGTGC CACCACCTCA GTATTTCTCT 
CCAGCCGTGA TCTTGCCGAG CCTCGCTGCC CCACTCCCCC CTGCGTCCCC AGCCTTGCCT 
CTGCAGGCTG TGAAGCTGCC CCACCCCCCT GGGGCGCCCC TGGCCATGCC CTGCCGGACC 
ATTGTGCCAA ATGCACCGGC CACTATCCCC CTGCTGGCCG TAGCCCCACC GGGCGTGGCT 
GCCCTGTCCA TTCATTCTGC CGTGGCCCAG CTCCCAGGCC AACCTGTGTA CCCAGCGGCC 
TTCCCACAGA TGGCGCCTAC TGACGTCCCT CCTTCCCCCC ATCACACGGT GCAGAATATG 
AGGGCCACCC CTCCACAGCC GGCACTGCCT CCACAACCCA CACTGCCCCC ACAACCCGTG 
CTGCCCCCGC AACCCACGCT GCCCCCTCAA CCTGTGTTGC CCCCGCAACC CACACGGCCC 
CCTCAACCTG TGCTGCCCCC GCAACCCATG CTGCCCCCAC AACCTGTGCT GCCCCCGCAG 
CCGGCACTGC CTGTGCGCCC TGAGCCCCTC CAGCCCCACC TTCCTGAACA AGCTGCTCCA 
GCTGCTACAC CAGGGAGCCA GATTCTGCTT GGCCACCCAG CTCCCTATGC TGTGGACGTC 
GCCGCTCAGG TCCCCACCGT GCCTGTGCCA CCGGCTGCGG TCCTCTCGCC GCCTCTGCCG 
GAAGTGCTGC TGCCTGCCGC CCCTGAGCTC CTGCCTCAGT TCCCCAGCTC CCTGGCCACG 
GTGTCTGCCT CTGTGCAGAG TGTGCCCACC CAGACTGCCA CACTTCTGCC ACCAGCAAAC 
CCACCGCTGC CTGGCGGGCC CGGGATCGCC AGCCCTTGCC CAACTGTCCA GCTGACGGTG 
GAACCAGTCC AAGAGGAGCA GGCCTCACAG GACAAGCCGC CCGGCCTCCC GCAGAGCTGT 
GAGAGCTATG GAGGTTCTGA TGTCACTTCT GGAAAAGAGC TGAGTGACAG CTGTGAAGGC 
GCCTTTGGAG GGGGCAGGCT GGAGGGCAGG GCAGCCCGAA AACACCACCG CAGGTCCACG 
CGTGCGCGCT CCCGGCAGGA GAGGGCCAGC CGGCCCCGGC TTACCATCTT GAACGTGTGC 
AACACTGGGG ACAAGATGGT GGAGTGCCAG CTGGAGACGC ACAACCACAA GATGGTGACC 
TTCAAGTTCG ACTTGGACGG GGACGCACCC GATGAAATTG CCACGTATAT GGTGGAGCAT 
GACTTTATCC TGCAGGCCGA GCGGGAAACG TTCATCGAGC AGATGAAGGA TGTCATGGAC 
AAGGCAGAGG ACATGCTCAG CGAGGACACA GACGCCGACC GTGGCTCCGA CCCAGGGACC 
AGCCCGCCAC ACCTCAGCAC CTGCGGCCTG GGCACCGGGG AGGAGAGCCG ACAATCCCAA 
GCCAACGCCC CCGTGTATCA GCAGAACGTC CTGCACACCG GGAAGAGGTG GTTCATCATC 
TGTCCGGTGG CTGAGCACCC CGCCCCCGAG GCCCCTGAAT CTTCGCCCCC ACTTCCTCTA 
AGCTCCCTGC CGCCAGAAGC CAGCCAAGAT TCAGCGCCCT ATAAAGACCA GCTGTCCTCG 
AAGGAACAAC CCAGCTTTCT AGCCAGTCAG CAGCTCCTGA GCCAGGCGGG CCCCAGCAAC 
CCTCCTGGGG CACCCCCAGC CCCTTTGGCC CCCTCCTCCC CTCCTGTGAC TGCTCTGCCC 
CAAGATGGAG CAGCTCCAGC CACCAGCACC ATGCCAGAGC CAGCGTCAGG AACTGCCAGC 
CAGGCAGGGG GTCCAGGGAC ACCTCAGGGG CTGACCAGTG AGCTCGAGAC GTCTCAGCCA 
CTAGCGGAGA CTCACGAGGC CCCGCTTGCT GTGCAGCCCC TCGTGGTGGG CCTAGCACCT 
TGCACTCCAG CTCCAGAGGC TGCCTCAACC AGGGACGCCA GTGCCCCAAG GGAGCCCCTG 
CCACCTCCTG CACCTGAGCC CAGCCCCCAC AGCGGGACCC CACAGCCCGC CTTGGGTCAA 
CCTGCTCCCC TGCTTCCTGC CGCAGTGGGG GCCGTCAGCC TGGCCACCTC CCAGCTCCCA 
AGCCCACCCC TGGGGCCCAC CGTCCCCCCA CAGCCACCCT CGGCCCTGGA GTCGGATGGG 
GAAGGGCCGC CCCCCAGGGT GGGCTTTGTG GACAGCACCA TCAAGAGCCT GGACGAGAAG 
CTGCGGACTC TGCTCTACCA GGAGCACGTG CCCACCTCCT CAGCCTCAGC TGGGACCCCT 
GTGGAGGTGG GCGACAGAGA CTTCACCCTG GAGCCCCTGA GAGGGGACCA GCCCCGCTCA 
GAGGTCTGCG GGGGGGACCT GGCCCTGCCC CCAGTGCCTA AGGAGGCGGT CTCAGGGCGT 
GTCCAGCTGC CCCAGCCCTT GGTGGAGAAG TCAGAACTGG CCCCCACTCG AGGGGCCGTG 
ATGGAGCAGG GCACGTCCTC GTCAATGACA GCAGAGTCGT CTCCCAGGAG TATGCTAGGC 
TATGACAGAG ATGGAAGGCA GGTGGCCTCA GACTCCCATG TGGTCCCCAG CGTCCCCCAG 
GATGTACCTG CTTTTGTGAG ACCTGCACGT GTGGAGCCCA CAGACAGGGA TGGTGGAGAA 
GCTGGAGAAA GCTCGGCAGA GCCCCCGCCG AGTGACATGG GCACAGTGGG GGGCCAGGCT 
AGCCACCCCC AGACACTCGG CGCTCGAGCT TTGGGGTCCC CTCGGAAACG TCCAGAGCAG 
CAGGATGTCA GCTCACCAGC CAAGACTGTG GGCCGTTTCT CGGTGGTCAG CACTCAGGAC 
GAGTGGACCC TGGCCTCCCC CCACAGCCTG AGATACTCTG CCCCACCCGA CGTCTACCTG 
GACGAGGCCC CCTCCAGCCC CGACGTGAAG CTGGCAGTGC GGCGGGCGCA GACGGCCTCC 
TCCATCGAGG TCGGCGTGGG CGAGCCCGTG TCCAGCGACT CTGGGGACGA GGGCCCTCGG 
GCGAGACCCC CGGTGCAGAA GCAGGCGTCC CTGCCCGTGA GTGGCAGCGT GGCTGGCGAC 
TTCGTGAAGA AGGCCACCGC CTTCCTGCAG AGGCCTTCTC GGGCCGGCTC GCTGGGCCCC 
GAGACACCCA GCAGGGTGGG CATGAAGGTC CCCACGATCA GCGTGACCTC CTTCCATTCC 
CAGTCGTCCT ACATCAGCAG CGACAATGAT TCGGAGCTCG AGGATGCTGA CATAAAGAAG 
GAGCTGCAGA GTCTGCGGGA GAAGCACCTG AAGGAGATCT CGGAGCTGCA GAGCCAGCAG 
AAGCAGGAGA TCGAAGCTCT GTACCGCCGC CTGGGCAAGC CACTGCCCCC CAACGTGGGC 
TTCTTCCACA CGGCACCCCC CACTGGCCGC CGGAGAAAAA CCAGCAAGAG CAAGCTGAAG 
GCAGGCAAGC TGCTAAATCC CCTGGTGCGG CAGCTCAAGG TCGTGGCCTC CAGCACAGGT 
CACTTGGCTG ACTCCAGCAG AGGCCCTCCC GCTAAGGACC CTGCCCAAGC CAGTGTGGGG 
CTCACTGCAG ACAGCACGGG CCTGAGCGGG AAGGCAGTGC AGACCCAGCA GCCCTGCTCC 
GTCCGGGCCT CCCTGTCTTC GGACATCTGC TCCGGCTTAG CCAGTGATGG AGGCGGAGCG 
CGTGGCCAAG GCTGGACGGT TTACCACCCA ACGTCTGAGA GAGTGACCTA TAAGTCTAGT 
AGCAAACCTC GTGCTCGATT CCTCAGTGGA CCCGTATCTG TGTCCATCTG GTCTGCCCTG 
AAGCGTCTCT GCCTAGGCAA AGAACACAGC AGTAGGTCCT CCACCAGCAG CCTGGCCCCA 
GGCCCTGAGC CAGGCCCCCA GCCCGCCCTG CACGTCCAGG CGCAGGTGAA CAACAGCAAC 
AACAAGAAGG GTACCTTCAC GGACGACCTG CACAAGCTGG TGGACGAGTG GACGAGCAAG 
ACGGTGGGGG CCGCGCAGCT GAAGCCCACG CTCAACCAGC TGAAGCAGAC CCAGAAGCTG 
CAAGACATGG AGGCCCAGGC AGGCTGGGCT GCCCCTGGCG AGGCGCGGGC TTCTGTGCGG 
TTGACTCCTT TTCCTCCCCG CCTGGAGATC CCCGTGGTGT CGACTGGAAG CATGGAGGCA 
CCTTGGGGAG ATGACCGCAC CTCGAGCAGG AGTGGGGATG CCACGTCTGC CCCCAGCGCC 
CGGCCCTCTG TCCACCACGG TCATTCCCGG AGCCGCCCCG ACCCTGTCCG TGCCCACACC 
AGATGGCGCC CTCGGAACCG CCCGGAGAAA CCAGGTGTGG TTTGGCCTCC GAGTCCCCCC 
CACCGCCTGC TGTGGGCACA GCACTCAGCC GCGAGGGGGA CAGCGGGTGG GCAGCAAGAC 
TGCTTCCTTT GCGGCTTCAG ACCCTGTTCG CTCCTAGGTT CCTGTGGTCC ACGCGCCGTC 
TCCACACCCA CTTCCTATAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518940.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517242.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X30, mRNA.

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CloneID	OHu71660
Clone ID Related Accession (Same CDS sequence)	XM_011518940.2 , XM_011518939.2
Accession Version	XM_011518940.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAG GCCTGCAGCA CCCCAACATC GTGCGCTTCT ACGACTTCTG GGAGTCCAGC 
GCCAAGGGCA AGCGGTGCAT TGTGCTGGTG ACGGAGCTGA TGACCTCAGG GACGCTGAAG 
ACATACCTGA AGCGGTTCAA GGTGATGAAG CCCAAGGTTC TCCGCAGCTG GTGCCGGCAG 
ATCCTGAAGG GCCTGCTGTT CCTGCACACA AGGACGCCAC CCATCATCCA CCGAGACCTG 
AAATGTGACA ATATTTTCAT CACCGGACCA ACTGGGTCTG TGAAGATTGG CGACTTGGGC 
CTGGCCACTC TGAAAAGAGC GTCATTTGCC AAAAGTGTGA TAGGTACTCC CGAGTTCATG 
GCGCCCGAGA TGTACGAGGA GCACTACGAT GAGTCCGTGG ACGTCTATGC CTTTGGGATG 
TGCATGCTGG AGATGGCCAC CTCGGAGTAC CCCTACTCGG AGTGCCAGAA TGCGGCCCAG 
ATCTACCGCA AGGTCACCTG TGGTATCAAG CCGGCCAGCT TTGAGAAAGT GCACGATCCT 
GAAATCAAGG AGATTATTGG GGAGTGTATC TGCAAAAACA AGGAGGAAAG GTACGAGATC 
AAAGACCTGC TGAGCCACGC CTTCTTCGCA GAGGACACAG GCGTGAGGGT GGAGCTCGCG 
GAGGAGGACC ACGGCAGGAA GTCCACCATC GCCCTGAGGC TCTGGGTGGA AGACCCCAAG 
AAACTGAAGG GAAAGCCCAA GGACAATGGA GCCATAGAGT TCACCTTCGA CCTGGAGAAG 
GAGACGCCGG ATGAGGTGGC CCAAGAGATG ATTGAGTCTG GATTCTTCCA CGAGAGTGAC 
GTCAAGATCG TGGCCAAGTC CATCCGTGAC CGCGTGGCCT TGATCCAGTG GCGGCGGGAG 
AGGATCTGGC CCGCGCTGCA GCCCAAGGAG CAGCAGGATG TGGGCAGCCC GGACAAGGCC 
AGGGGTCCGC CGGTGCCCCT GCAGGTCCAG GTGACCTACC ATGCACAGGC TGGGCAGCCC 
GGGCCACCAG AGCCCGAGGA GCCGGAGGCC GACCAGCACC TCCTGCCACC TACGTTGCCG 
ACCAGCGCCA CCTCCCTGGC CTCGGACAGC ACCTTCGACA GCGGCCAGGG CTCTACCGTG 
TACTCAGACT CGCAGAGCAG CCAGCAGAGC GTGATGCTTG GCTCCCTTGC CGACGCAGCG 
CCGTCCCCGG CCCAGTGTGT GTGCAGCCCC CCTGTGAGCG AGGGGCCCGT CCTGCCGCAG 
AGCCTGCCCT CGCTGGGGGC CTACCAGCAG CCCACGGCTG CACCTGGCTT GCCGGTGGGC 
TCTGTCCCGG CCCCCGCCTG CCCTCCGTCC CTCCAGCAGC ACTTCCCGGA TCCGGCCATG 
AGCTTCGCCC CCGTGCTGCC GCCGCCCAGC ACCCCCATGC CCACGGGCCC AGGCCAGCCA 
GCACCCCCCG GCCAGCAGCC TCCTCCGCTG GCCCAGCCGA CACCCCTGCC GCAGGTCCTG 
GCCCCACAGC CCGTGGTCCC CCTCCAGCCG GTTCCCCCCC ACCTGCCACC GTACCTGGCT 
CCAGCCTCCC AGGTGGGGGC CCCCGCTCAG CTGAAGCCCC TCCAGATGCC ACAGGCGCCC 
CTGCAGCCGC TTGCTCAAGT CCCTCCGCAG ATGCCCCCGA TTCCTGTTGT GCCCCCCATC 
ACGCCCCTGG CGGGAATCGA CGGCCTCCCT CCGGCCCTCC CAGACCTGCC GACCGCGACT 
GTGCCTCCCG TGCCACCACC TCAGTATTTC TCTCCAGCCG TGATCTTGCC GAGCCTCGCT 
GCCCCACTCC CCCCTGCGTC CCCAGCCTTG CCTCTGCAGG CTGTGAAGCT GCCCCACCCC 
CCTGGGGCGC CCCTGGCCAT GCCCTGCCGG ACCATTGTGC CAAATGCACC GGCCACTATC 
CCCCTGCTGG CCGTAGCCCC ACCGGGCGTG GCTGCCCTGT CCATTCATTC TGCCGTGGCC 
CAGCTCCCAG GCCAACCTGT GTACCCAGCG GCCTTCCCAC AGATGGCGCC TACTGACGTC 
CCTCCTTCCC CCCATCACAC GGTGCAGAAT ATGAGGGCCA CCCCTCCACA GCCGGCACTG 
CCTCCACAAC CCACACTGCC CCCACAACCC GTGCTGCCCC CGCAACCCAC GCTGCCCCCT 
CAACCTGTGT TGCCCCCGCA ACCCACACGG CCCCCTCAAC CTGTGCTGCC CCCGCAACCC 
ATGCTGCCCC CACAACCTGT GCTGCCCCCG CAGCCGGCAC TGCCTGTGCG CCCTGAGCCC 
CTCCAGCCCC ACCTTCCTGA ACAAGCTGCT CCAGCTGCTA CACCAGGGAG CCAGATTCTG 
CTTGGCCACC CAGCTCCCTA TGCTGTGGAC GTCGCCGCTC AGGTCCCCAC CGTGCCTGTG 
CCACCGGCTG CGGTCCTCTC GCCGCCTCTG CCGGAAGTGC TGCTGCCTGC CGCCCCTGAG 
CTCCTGCCTC AGTTCCCCAG CTCCCTGGCC ACGGTGTCTG CCTCTGTGCA GAGTGTGCCC 
ACCCAGACTG CCACACTTCT GCCACCAGCA AACCCACCGC TGCCTGGCGG GCCCGGGATC 
GCCAGCCCTT GCCCAACTGT CCAGCTGACG GTGGAACCAG TCCAAGAGGA GCAGGCCTCA 
CAGGACAAGC CGCCCGGCCT CCCGCAGAGC TGTGAGAGCT ATGGAGGTTC TGATGTCACT 
TCTGGAAAAG AGCTGAGTGA CAGCTGTGAA GGCGCCTTTG GAGGGGGCAG GCTGGAGGGC 
AGGGCAGCCC GAAAACACCA CCGCAGGTCC ACGCGTGCGC GCTCCCGGCA GGAGAGGGCC 
AGCCGGCCCC GGCTTACCAT CTTGAACGTG TGCAACACTG GGGACAAGAT GGTGGAGTGC 
CAGCTGGAGA CGCACAACCA CAAGATGGTG ACCTTCAAGT TCGACTTGGA CGGGGACGCA 
CCCGATGAAA TTGCCACGTA TATGGTGGAG CATGACTTTA TCCTGCAGGC CGAGCGGGAA 
ACGTTCATCG AGCAGATGAA GGATGTCATG GACAAGGCAG AGGACATGCT CAGCGAGGAC 
ACAGACGCCG ACCGTGGCTC CGACCCAGGG ACCAGCCCGC CACACCTCAG CACCTGCGGC 
CTGGGCACCG GGGAGGAGAG CCGACAATCC CAAGCCAACG CCCCCGTGTA TCAGCAGAAC 
GTCCTGCACA CCGGGAAGAG GTGGTTCATC ATCTGTCCGG TGGCTGAGCA CCCCGCCCCC 
GAGGCCCCTG AATCTTCGCC CCCACTTCCT CTAAGCTCCC TGCCGCCAGA AGCCAGCCAA 
GATTCAGCGC CCTATAAAGA CCAGCTGTCC TCGAAGGAAC AACCCAGCTT TCTAGCCAGT 
CAGCAGCTCC TGAGCCAGGC GGGCCCCAGC AACCCTCCTG GGGCACCCCC AGCCCCTTTG 
GCCCCCTCCT CCCCTCCTGT GACTGCTCTG CCCCAAGATG GAGCAGCTCC AGCCACCAGC 
ACCATGCCAG AGCCAGCGTC AGGAACTGCC AGCCAGGCAG GGGGTCCAGG GACACCTCAG 
GGGCTGACCA GTGAGCTCGA GACGTCTCAG CCACTAGCGG AGACTCACGA GGCCCCGCTT 
GCTGTGCAGC CCCTCGTGGT GGGCCTAGCA CCTTGCACTC CAGCTCCAGA GGCTGCCTCA 
ACCAGGGACG CCAGTGCCCC AAGGGAGCCC CTGCCACCTC CTGCACCTGA GCCCAGCCCC 
CACAGCGGGA CCCCACAGCC CGCCTTGGGT CAACCTGCTC CCCTGCTTCC TGCCGCAGTG 
GGGGCCGTCA GCCTGGCCAC CTCCCAGCTC CCAAGCCCAC CCCTGGGGCC CACCGTCCCC 
CCACAGCCAC CCTCGGCCCT GGAGTCGGAT GGGGAAGGGC CGCCCCCCAG GGTGGGCTTT 
GTGGACAGCA CCATCAAGAG CCTGGACGAG AAGCTGCGGA CTCTGCTCTA CCAGGAGCAC 
GTGCCCACCT CCTCAGCCTC AGCTGGGACC CCTGTGGAGG TGGGCGACAG AGACTTCACC 
CTGGAGCCCC TGAGAGGGGA CCAGCCCCGC TCAGAGGTCT GCGGGGGGGA CCTGGCCCTG 
CCCCCAGTGC CTAAGGAGGC GGTCTCAGGG CGTGTCCAGC TGCCCCAGCC CTTGGTGGAG 
AAGTCAGAAC TGGCCCCCAC TCGAGGGGCC GTGATGGAGC AGGGCACGTC CTCGTCAATG 
ACAGCAGAGT CGTCTCCCAG GAGTATGCTA GGCTATGACA GAGATGGAAG GCAGGTGGCC 
TCAGACTCCC ATGTGGTCCC CAGCGTCCCC CAGGATGTAC CTGCTTTTGT GAGACCTGCA 
CGTGTGGAGC CCACAGACAG GGATGGTGGA GAAGCTGGAG AAAGCTCGGC AGAGCCCCCG 
CCGAGTGACA TGGGCACAGT GGGGGGCCAG GCTAGCCACC CCCAGACACT CGGCGCTCGA 
GCTTTGGGGT CCCCTCGGAA ACGTCCAGAG CAGCAGGATG TCAGCTCACC AGCCAAGACT 
GTGGGCCGTT TCTCGGTGGT CAGCACTCAG GACGAGTGGA CCCTGGCCTC CCCCCACAGC 
CTGAGATACT CTGCCCCACC CGACGTCTAC CTGGACGAGG CCCCCTCCAG CCCCGACGTG 
AAGCTGGCAG TGCGGCGGGC GCAGACGGCC TCCTCCATCG AGGTCGGCGT GGGCGAGCCC 
GTGTCCAGCG ACTCTGGGGA CGAGGGCCCT CGGGCGAGAC CCCCGGTGCA GAAGCAGGCG 
TCCCTGCCCG TGAGTGGCAG CGTGGCTGGC GACTTCGTGA AGAAGGCCAC CGCCTTCCTG 
CAGAGGCCTT CTCGGGCCGG CTCGCTGGGC CCCGAGACAC CCAGCAGGGT GGGCATGAAG 
GTCCCCACGA TCAGCGTGAC CTCCTTCCAT TCCCAGTCGT CCTACATCAG CAGCGACAAT 
GATTCGGAGC TCGAGGATGC TGACATAAAG AAGGAGCTGC AGAGTCTGCG GGAGAAGCAC 
CTGAAGGAGA TCTCGGAGCT GCAGAGCCAG CAGAAGCAGG AGATCGAAGC TCTGTACCGC 
CGCCTGGGCA AGCCACTGCC CCCCAACGTG GGCTTCTTCC ACACGGCACC CCCCACTGGC 
CGCCGGAGAA AAACCAGCAA GAGCAAGCTG AAGGCAGGCA AGCTGCTAAA TCCCCTGGTG 
CGGCAGCTCA AGGTCGTGGC CTCCAGCACA GGTCACTTGG CTGACTCCAG CAGAGGCCCT 
CCCGCTAAGG ACCCTGCCCA AGCCAGTGTG GGGCTCACTG CAGACAGCAC GGGCCTGAGC 
GGGAAGGCAG TGCAGACCCA GCAGCCCTGC TCCGTCCGGG CCTCCCTGTC TTCGGACATC 
TGCTCCGGCT TAGCCAGTGA TGGAGGCGGA GCGCGTGGCC AAGGCTGGAC GGTTTACCAC 
CCAACGTCTG AGAGAGTGAC CTATAAGTCT AGTAGCAAAC CTCGTGCTCG ATTCCTCAGT 
GGACCCGTAT CTGTGTCCAT CTGGTCTGCC CTGAAGCGTC TCTGCCTAGG CAAAGAACAC 
AGCAGTAGGT CCTCCACCAG CAGCCTGGCC CCAGGCCCTG AGCCAGGCCC CCAGCCCGCC 
CTGCACGTCC AGGCGCAGGT GAACAACAGC AACAACAAGA AGGGTACCTT CACGGACGAC 
CTGCACAAGC TGGTGGACGA GTGGACGAGC AAGACGGTGG GGGCCGCGCA GCTGAAGCCC 
ACGCTCAACC AGCTGAAGCA GACCCAGAAG CTGCAAGACA TGGAGGCCCA GGCAGGCTGG 
GCTGCCCCTG GCGAGGCGCG GGCTTCTGTG CGGTTGACTC CTTTTCCTCC CCGCCTGGAG 
ATCCCCGTGG TGTCGACTGG AAGCATGGAG GCACCTTGGG GAGATGACCG CACCTCGAGC 
AGGAGTGGGG ATGCCACGTC TGCCCCCAGC GCCCGGCCCT CTGTCCACCA CGGTCATTCC 
CGGAGCCGCC CCGACCCTGT CCGTGCCCAC ACCAGATGGC GCCCTCGGAA CCGCCCGGAG 
AAACCAGGTG TGGTTTGGCC TCCGAGTCCC CCCCACCGCC TGCTGTGGGC ACAGCACTCA 
GCCGCGAGGG GGACAGCGGG TGGGCAGCAA GACTGCTTCC TTTGCGGCTT CAGACCCTGT 
TCGCTCCTAG GTTCCTGTGG TCCACGCGCC GTCTCCACAC CCACTTCCTA TACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517242.1
CDS	320..6496
Translation	MLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518940.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518940.2

ORF Insert Sequence:

ATGCTGAAAG GCCTGCAGCA CCCCAACATC GTGCGCTTCT ACGACTTCTG GGAGTCCAGC 
GCCAAGGGCA AGCGGTGCAT TGTGCTGGTG ACGGAGCTGA TGACCTCAGG GACGCTGAAG 
ACATACCTGA AGCGGTTCAA GGTGATGAAG CCCAAGGTTC TCCGCAGCTG GTGCCGGCAG 
ATCCTGAAGG GCCTGCTGTT CCTGCACACA AGGACGCCAC CCATCATCCA CCGAGACCTG 
AAATGTGACA ATATTTTCAT CACCGGACCA ACTGGGTCTG TGAAGATTGG CGACTTGGGC 
CTGGCCACTC TGAAAAGAGC GTCATTTGCC AAAAGTGTGA TAGGTACTCC CGAGTTCATG 
GCGCCCGAGA TGTACGAGGA GCACTACGAT GAGTCCGTGG ACGTCTATGC CTTTGGGATG 
TGCATGCTGG AGATGGCCAC CTCGGAGTAC CCCTACTCGG AGTGCCAGAA TGCGGCCCAG 
ATCTACCGCA AGGTCACCTG TGGTATCAAG CCGGCCAGCT TTGAGAAAGT GCACGATCCT 
GAAATCAAGG AGATTATTGG GGAGTGTATC TGCAAAAACA AGGAGGAAAG GTACGAGATC 
AAAGACCTGC TGAGCCACGC CTTCTTCGCA GAGGACACAG GCGTGAGGGT GGAGCTCGCG 
GAGGAGGACC ACGGCAGGAA GTCCACCATC GCCCTGAGGC TCTGGGTGGA AGACCCCAAG 
AAACTGAAGG GAAAGCCCAA GGACAATGGA GCCATAGAGT TCACCTTCGA CCTGGAGAAG 
GAGACGCCGG ATGAGGTGGC CCAAGAGATG ATTGAGTCTG GATTCTTCCA CGAGAGTGAC 
GTCAAGATCG TGGCCAAGTC CATCCGTGAC CGCGTGGCCT TGATCCAGTG GCGGCGGGAG 
AGGATCTGGC CCGCGCTGCA GCCCAAGGAG CAGCAGGATG TGGGCAGCCC GGACAAGGCC 
AGGGGTCCGC CGGTGCCCCT GCAGGTCCAG GTGACCTACC ATGCACAGGC TGGGCAGCCC 
GGGCCACCAG AGCCCGAGGA GCCGGAGGCC GACCAGCACC TCCTGCCACC TACGTTGCCG 
ACCAGCGCCA CCTCCCTGGC CTCGGACAGC ACCTTCGACA GCGGCCAGGG CTCTACCGTG 
TACTCAGACT CGCAGAGCAG CCAGCAGAGC GTGATGCTTG GCTCCCTTGC CGACGCAGCG 
CCGTCCCCGG CCCAGTGTGT GTGCAGCCCC CCTGTGAGCG AGGGGCCCGT CCTGCCGCAG 
AGCCTGCCCT CGCTGGGGGC CTACCAGCAG CCCACGGCTG CACCTGGCTT GCCGGTGGGC 
TCTGTCCCGG CCCCCGCCTG CCCTCCGTCC CTCCAGCAGC ACTTCCCGGA TCCGGCCATG 
AGCTTCGCCC CCGTGCTGCC GCCGCCCAGC ACCCCCATGC CCACGGGCCC AGGCCAGCCA 
GCACCCCCCG GCCAGCAGCC TCCTCCGCTG GCCCAGCCGA CACCCCTGCC GCAGGTCCTG 
GCCCCACAGC CCGTGGTCCC CCTCCAGCCG GTTCCCCCCC ACCTGCCACC GTACCTGGCT 
CCAGCCTCCC AGGTGGGGGC CCCCGCTCAG CTGAAGCCCC TCCAGATGCC ACAGGCGCCC 
CTGCAGCCGC TTGCTCAAGT CCCTCCGCAG ATGCCCCCGA TTCCTGTTGT GCCCCCCATC 
ACGCCCCTGG CGGGAATCGA CGGCCTCCCT CCGGCCCTCC CAGACCTGCC GACCGCGACT 
GTGCCTCCCG TGCCACCACC TCAGTATTTC TCTCCAGCCG TGATCTTGCC GAGCCTCGCT 
GCCCCACTCC CCCCTGCGTC CCCAGCCTTG CCTCTGCAGG CTGTGAAGCT GCCCCACCCC 
CCTGGGGCGC CCCTGGCCAT GCCCTGCCGG ACCATTGTGC CAAATGCACC GGCCACTATC 
CCCCTGCTGG CCGTAGCCCC ACCGGGCGTG GCTGCCCTGT CCATTCATTC TGCCGTGGCC 
CAGCTCCCAG GCCAACCTGT GTACCCAGCG GCCTTCCCAC AGATGGCGCC TACTGACGTC 
CCTCCTTCCC CCCATCACAC GGTGCAGAAT ATGAGGGCCA CCCCTCCACA GCCGGCACTG 
CCTCCACAAC CCACACTGCC CCCACAACCC GTGCTGCCCC CGCAACCCAC GCTGCCCCCT 
CAACCTGTGT TGCCCCCGCA ACCCACACGG CCCCCTCAAC CTGTGCTGCC CCCGCAACCC 
ATGCTGCCCC CACAACCTGT GCTGCCCCCG CAGCCGGCAC TGCCTGTGCG CCCTGAGCCC 
CTCCAGCCCC ACCTTCCTGA ACAAGCTGCT CCAGCTGCTA CACCAGGGAG CCAGATTCTG 
CTTGGCCACC CAGCTCCCTA TGCTGTGGAC GTCGCCGCTC AGGTCCCCAC CGTGCCTGTG 
CCACCGGCTG CGGTCCTCTC GCCGCCTCTG CCGGAAGTGC TGCTGCCTGC CGCCCCTGAG 
CTCCTGCCTC AGTTCCCCAG CTCCCTGGCC ACGGTGTCTG CCTCTGTGCA GAGTGTGCCC 
ACCCAGACTG CCACACTTCT GCCACCAGCA AACCCACCGC TGCCTGGCGG GCCCGGGATC 
GCCAGCCCTT GCCCAACTGT CCAGCTGACG GTGGAACCAG TCCAAGAGGA GCAGGCCTCA 
CAGGACAAGC CGCCCGGCCT CCCGCAGAGC TGTGAGAGCT ATGGAGGTTC TGATGTCACT 
TCTGGAAAAG AGCTGAGTGA CAGCTGTGAA GGCGCCTTTG GAGGGGGCAG GCTGGAGGGC 
AGGGCAGCCC GAAAACACCA CCGCAGGTCC ACGCGTGCGC GCTCCCGGCA GGAGAGGGCC 
AGCCGGCCCC GGCTTACCAT CTTGAACGTG TGCAACACTG GGGACAAGAT GGTGGAGTGC 
CAGCTGGAGA CGCACAACCA CAAGATGGTG ACCTTCAAGT TCGACTTGGA CGGGGACGCA 
CCCGATGAAA TTGCCACGTA TATGGTGGAG CATGACTTTA TCCTGCAGGC CGAGCGGGAA 
ACGTTCATCG AGCAGATGAA GGATGTCATG GACAAGGCAG AGGACATGCT CAGCGAGGAC 
ACAGACGCCG ACCGTGGCTC CGACCCAGGG ACCAGCCCGC CACACCTCAG CACCTGCGGC 
CTGGGCACCG GGGAGGAGAG CCGACAATCC CAAGCCAACG CCCCCGTGTA TCAGCAGAAC 
GTCCTGCACA CCGGGAAGAG GTGGTTCATC ATCTGTCCGG TGGCTGAGCA CCCCGCCCCC 
GAGGCCCCTG AATCTTCGCC CCCACTTCCT CTAAGCTCCC TGCCGCCAGA AGCCAGCCAA 
GATTCAGCGC CCTATAAAGA CCAGCTGTCC TCGAAGGAAC AACCCAGCTT TCTAGCCAGT 
CAGCAGCTCC TGAGCCAGGC GGGCCCCAGC AACCCTCCTG GGGCACCCCC AGCCCCTTTG 
GCCCCCTCCT CCCCTCCTGT GACTGCTCTG CCCCAAGATG GAGCAGCTCC AGCCACCAGC 
ACCATGCCAG AGCCAGCGTC AGGAACTGCC AGCCAGGCAG GGGGTCCAGG GACACCTCAG 
GGGCTGACCA GTGAGCTCGA GACGTCTCAG CCACTAGCGG AGACTCACGA GGCCCCGCTT 
GCTGTGCAGC CCCTCGTGGT GGGCCTAGCA CCTTGCACTC CAGCTCCAGA GGCTGCCTCA 
ACCAGGGACG CCAGTGCCCC AAGGGAGCCC CTGCCACCTC CTGCACCTGA GCCCAGCCCC 
CACAGCGGGA CCCCACAGCC CGCCTTGGGT CAACCTGCTC CCCTGCTTCC TGCCGCAGTG 
GGGGCCGTCA GCCTGGCCAC CTCCCAGCTC CCAAGCCCAC CCCTGGGGCC CACCGTCCCC 
CCACAGCCAC CCTCGGCCCT GGAGTCGGAT GGGGAAGGGC CGCCCCCCAG GGTGGGCTTT 
GTGGACAGCA CCATCAAGAG CCTGGACGAG AAGCTGCGGA CTCTGCTCTA CCAGGAGCAC 
GTGCCCACCT CCTCAGCCTC AGCTGGGACC CCTGTGGAGG TGGGCGACAG AGACTTCACC 
CTGGAGCCCC TGAGAGGGGA CCAGCCCCGC TCAGAGGTCT GCGGGGGGGA CCTGGCCCTG 
CCCCCAGTGC CTAAGGAGGC GGTCTCAGGG CGTGTCCAGC TGCCCCAGCC CTTGGTGGAG 
AAGTCAGAAC TGGCCCCCAC TCGAGGGGCC GTGATGGAGC AGGGCACGTC CTCGTCAATG 
ACAGCAGAGT CGTCTCCCAG GAGTATGCTA GGCTATGACA GAGATGGAAG GCAGGTGGCC 
TCAGACTCCC ATGTGGTCCC CAGCGTCCCC CAGGATGTAC CTGCTTTTGT GAGACCTGCA 
CGTGTGGAGC CCACAGACAG GGATGGTGGA GAAGCTGGAG AAAGCTCGGC AGAGCCCCCG 
CCGAGTGACA TGGGCACAGT GGGGGGCCAG GCTAGCCACC CCCAGACACT CGGCGCTCGA 
GCTTTGGGGT CCCCTCGGAA ACGTCCAGAG CAGCAGGATG TCAGCTCACC AGCCAAGACT 
GTGGGCCGTT TCTCGGTGGT CAGCACTCAG GACGAGTGGA CCCTGGCCTC CCCCCACAGC 
CTGAGATACT CTGCCCCACC CGACGTCTAC CTGGACGAGG CCCCCTCCAG CCCCGACGTG 
AAGCTGGCAG TGCGGCGGGC GCAGACGGCC TCCTCCATCG AGGTCGGCGT GGGCGAGCCC 
GTGTCCAGCG ACTCTGGGGA CGAGGGCCCT CGGGCGAGAC CCCCGGTGCA GAAGCAGGCG 
TCCCTGCCCG TGAGTGGCAG CGTGGCTGGC GACTTCGTGA AGAAGGCCAC CGCCTTCCTG 
CAGAGGCCTT CTCGGGCCGG CTCGCTGGGC CCCGAGACAC CCAGCAGGGT GGGCATGAAG 
GTCCCCACGA TCAGCGTGAC CTCCTTCCAT TCCCAGTCGT CCTACATCAG CAGCGACAAT 
GATTCGGAGC TCGAGGATGC TGACATAAAG AAGGAGCTGC AGAGTCTGCG GGAGAAGCAC 
CTGAAGGAGA TCTCGGAGCT GCAGAGCCAG CAGAAGCAGG AGATCGAAGC TCTGTACCGC 
CGCCTGGGCA AGCCACTGCC CCCCAACGTG GGCTTCTTCC ACACGGCACC CCCCACTGGC 
CGCCGGAGAA AAACCAGCAA GAGCAAGCTG AAGGCAGGCA AGCTGCTAAA TCCCCTGGTG 
CGGCAGCTCA AGGTCGTGGC CTCCAGCACA GGTCACTTGG CTGACTCCAG CAGAGGCCCT 
CCCGCTAAGG ACCCTGCCCA AGCCAGTGTG GGGCTCACTG CAGACAGCAC GGGCCTGAGC 
GGGAAGGCAG TGCAGACCCA GCAGCCCTGC TCCGTCCGGG CCTCCCTGTC TTCGGACATC 
TGCTCCGGCT TAGCCAGTGA TGGAGGCGGA GCGCGTGGCC AAGGCTGGAC GGTTTACCAC 
CCAACGTCTG AGAGAGTGAC CTATAAGTCT AGTAGCAAAC CTCGTGCTCG ATTCCTCAGT 
GGACCCGTAT CTGTGTCCAT CTGGTCTGCC CTGAAGCGTC TCTGCCTAGG CAAAGAACAC 
AGCAGTAGGT CCTCCACCAG CAGCCTGGCC CCAGGCCCTG AGCCAGGCCC CCAGCCCGCC 
CTGCACGTCC AGGCGCAGGT GAACAACAGC AACAACAAGA AGGGTACCTT CACGGACGAC 
CTGCACAAGC TGGTGGACGA GTGGACGAGC AAGACGGTGG GGGCCGCGCA GCTGAAGCCC 
ACGCTCAACC AGCTGAAGCA GACCCAGAAG CTGCAAGACA TGGAGGCCCA GGCAGGCTGG 
GCTGCCCCTG GCGAGGCGCG GGCTTCTGTG CGGTTGACTC CTTTTCCTCC CCGCCTGGAG 
ATCCCCGTGG TGTCGACTGG AAGCATGGAG GCACCTTGGG GAGATGACCG CACCTCGAGC 
AGGAGTGGGG ATGCCACGTC TGCCCCCAGC GCCCGGCCCT CTGTCCACCA CGGTCATTCC 
CGGAGCCGCC CCGACCCTGT CCGTGCCCAC ACCAGATGGC GCCCTCGGAA CCGCCCGGAG 
AAACCAGGTG TGGTTTGGCC TCCGAGTCCC CCCCACCGCC TGCTGTGGGC ACAGCACTCA 
GCCGCGAGGG GGACAGCGGG TGGGCAGCAA GACTGCTTCC TTTGCGGCTT CAGACCCTGT 
TCGCTCCTAG GTTCCTGTGG TCCACGCGCC GTCTCCACAC CCACTTCCTA TACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_011518939.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517241.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X29, mRNA.

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CloneID	OHu71660
Clone ID Related Accession (Same CDS sequence)	XM_011518940.2 , XM_011518939.2
Accession Version	XM_011518939.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAG GCCTGCAGCA CCCCAACATC GTGCGCTTCT ACGACTTCTG GGAGTCCAGC 
GCCAAGGGCA AGCGGTGCAT TGTGCTGGTG ACGGAGCTGA TGACCTCAGG GACGCTGAAG 
ACATACCTGA AGCGGTTCAA GGTGATGAAG CCCAAGGTTC TCCGCAGCTG GTGCCGGCAG 
ATCCTGAAGG GCCTGCTGTT CCTGCACACA AGGACGCCAC CCATCATCCA CCGAGACCTG 
AAATGTGACA ATATTTTCAT CACCGGACCA ACTGGGTCTG TGAAGATTGG CGACTTGGGC 
CTGGCCACTC TGAAAAGAGC GTCATTTGCC AAAAGTGTGA TAGGTACTCC CGAGTTCATG 
GCGCCCGAGA TGTACGAGGA GCACTACGAT GAGTCCGTGG ACGTCTATGC CTTTGGGATG 
TGCATGCTGG AGATGGCCAC CTCGGAGTAC CCCTACTCGG AGTGCCAGAA TGCGGCCCAG 
ATCTACCGCA AGGTCACCTG TGGTATCAAG CCGGCCAGCT TTGAGAAAGT GCACGATCCT 
GAAATCAAGG AGATTATTGG GGAGTGTATC TGCAAAAACA AGGAGGAAAG GTACGAGATC 
AAAGACCTGC TGAGCCACGC CTTCTTCGCA GAGGACACAG GCGTGAGGGT GGAGCTCGCG 
GAGGAGGACC ACGGCAGGAA GTCCACCATC GCCCTGAGGC TCTGGGTGGA AGACCCCAAG 
AAACTGAAGG GAAAGCCCAA GGACAATGGA GCCATAGAGT TCACCTTCGA CCTGGAGAAG 
GAGACGCCGG ATGAGGTGGC CCAAGAGATG ATTGAGTCTG GATTCTTCCA CGAGAGTGAC 
GTCAAGATCG TGGCCAAGTC CATCCGTGAC CGCGTGGCCT TGATCCAGTG GCGGCGGGAG 
AGGATCTGGC CCGCGCTGCA GCCCAAGGAG CAGCAGGATG TGGGCAGCCC GGACAAGGCC 
AGGGGTCCGC CGGTGCCCCT GCAGGTCCAG GTGACCTACC ATGCACAGGC TGGGCAGCCC 
GGGCCACCAG AGCCCGAGGA GCCGGAGGCC GACCAGCACC TCCTGCCACC TACGTTGCCG 
ACCAGCGCCA CCTCCCTGGC CTCGGACAGC ACCTTCGACA GCGGCCAGGG CTCTACCGTG 
TACTCAGACT CGCAGAGCAG CCAGCAGAGC GTGATGCTTG GCTCCCTTGC CGACGCAGCG 
CCGTCCCCGG CCCAGTGTGT GTGCAGCCCC CCTGTGAGCG AGGGGCCCGT CCTGCCGCAG 
AGCCTGCCCT CGCTGGGGGC CTACCAGCAG CCCACGGCTG CACCTGGCTT GCCGGTGGGC 
TCTGTCCCGG CCCCCGCCTG CCCTCCGTCC CTCCAGCAGC ACTTCCCGGA TCCGGCCATG 
AGCTTCGCCC CCGTGCTGCC GCCGCCCAGC ACCCCCATGC CCACGGGCCC AGGCCAGCCA 
GCACCCCCCG GCCAGCAGCC TCCTCCGCTG GCCCAGCCGA CACCCCTGCC GCAGGTCCTG 
GCCCCACAGC CCGTGGTCCC CCTCCAGCCG GTTCCCCCCC ACCTGCCACC GTACCTGGCT 
CCAGCCTCCC AGGTGGGGGC CCCCGCTCAG CTGAAGCCCC TCCAGATGCC ACAGGCGCCC 
CTGCAGCCGC TTGCTCAAGT CCCTCCGCAG ATGCCCCCGA TTCCTGTTGT GCCCCCCATC 
ACGCCCCTGG CGGGAATCGA CGGCCTCCCT CCGGCCCTCC CAGACCTGCC GACCGCGACT 
GTGCCTCCCG TGCCACCACC TCAGTATTTC TCTCCAGCCG TGATCTTGCC GAGCCTCGCT 
GCCCCACTCC CCCCTGCGTC CCCAGCCTTG CCTCTGCAGG CTGTGAAGCT GCCCCACCCC 
CCTGGGGCGC CCCTGGCCAT GCCCTGCCGG ACCATTGTGC CAAATGCACC GGCCACTATC 
CCCCTGCTGG CCGTAGCCCC ACCGGGCGTG GCTGCCCTGT CCATTCATTC TGCCGTGGCC 
CAGCTCCCAG GCCAACCTGT GTACCCAGCG GCCTTCCCAC AGATGGCGCC TACTGACGTC 
CCTCCTTCCC CCCATCACAC GGTGCAGAAT ATGAGGGCCA CCCCTCCACA GCCGGCACTG 
CCTCCACAAC CCACACTGCC CCCACAACCC GTGCTGCCCC CGCAACCCAC GCTGCCCCCT 
CAACCTGTGT TGCCCCCGCA ACCCACACGG CCCCCTCAAC CTGTGCTGCC CCCGCAACCC 
ATGCTGCCCC CACAACCTGT GCTGCCCCCG CAGCCGGCAC TGCCTGTGCG CCCTGAGCCC 
CTCCAGCCCC ACCTTCCTGA ACAAGCTGCT CCAGCTGCTA CACCAGGGAG CCAGATTCTG 
CTTGGCCACC CAGCTCCCTA TGCTGTGGAC GTCGCCGCTC AGGTCCCCAC CGTGCCTGTG 
CCACCGGCTG CGGTCCTCTC GCCGCCTCTG CCGGAAGTGC TGCTGCCTGC CGCCCCTGAG 
CTCCTGCCTC AGTTCCCCAG CTCCCTGGCC ACGGTGTCTG CCTCTGTGCA GAGTGTGCCC 
ACCCAGACTG CCACACTTCT GCCACCAGCA AACCCACCGC TGCCTGGCGG GCCCGGGATC 
GCCAGCCCTT GCCCAACTGT CCAGCTGACG GTGGAACCAG TCCAAGAGGA GCAGGCCTCA 
CAGGACAAGC CGCCCGGCCT CCCGCAGAGC TGTGAGAGCT ATGGAGGTTC TGATGTCACT 
TCTGGAAAAG AGCTGAGTGA CAGCTGTGAA GGCGCCTTTG GAGGGGGCAG GCTGGAGGGC 
AGGGCAGCCC GAAAACACCA CCGCAGGTCC ACGCGTGCGC GCTCCCGGCA GGAGAGGGCC 
AGCCGGCCCC GGCTTACCAT CTTGAACGTG TGCAACACTG GGGACAAGAT GGTGGAGTGC 
CAGCTGGAGA CGCACAACCA CAAGATGGTG ACCTTCAAGT TCGACTTGGA CGGGGACGCA 
CCCGATGAAA TTGCCACGTA TATGGTGGAG CATGACTTTA TCCTGCAGGC CGAGCGGGAA 
ACGTTCATCG AGCAGATGAA GGATGTCATG GACAAGGCAG AGGACATGCT CAGCGAGGAC 
ACAGACGCCG ACCGTGGCTC CGACCCAGGG ACCAGCCCGC CACACCTCAG CACCTGCGGC 
CTGGGCACCG GGGAGGAGAG CCGACAATCC CAAGCCAACG CCCCCGTGTA TCAGCAGAAC 
GTCCTGCACA CCGGGAAGAG GTGGTTCATC ATCTGTCCGG TGGCTGAGCA CCCCGCCCCC 
GAGGCCCCTG AATCTTCGCC CCCACTTCCT CTAAGCTCCC TGCCGCCAGA AGCCAGCCAA 
GATTCAGCGC CCTATAAAGA CCAGCTGTCC TCGAAGGAAC AACCCAGCTT TCTAGCCAGT 
CAGCAGCTCC TGAGCCAGGC GGGCCCCAGC AACCCTCCTG GGGCACCCCC AGCCCCTTTG 
GCCCCCTCCT CCCCTCCTGT GACTGCTCTG CCCCAAGATG GAGCAGCTCC AGCCACCAGC 
ACCATGCCAG AGCCAGCGTC AGGAACTGCC AGCCAGGCAG GGGGTCCAGG GACACCTCAG 
GGGCTGACCA GTGAGCTCGA GACGTCTCAG CCACTAGCGG AGACTCACGA GGCCCCGCTT 
GCTGTGCAGC CCCTCGTGGT GGGCCTAGCA CCTTGCACTC CAGCTCCAGA GGCTGCCTCA 
ACCAGGGACG CCAGTGCCCC AAGGGAGCCC CTGCCACCTC CTGCACCTGA GCCCAGCCCC 
CACAGCGGGA CCCCACAGCC CGCCTTGGGT CAACCTGCTC CCCTGCTTCC TGCCGCAGTG 
GGGGCCGTCA GCCTGGCCAC CTCCCAGCTC CCAAGCCCAC CCCTGGGGCC CACCGTCCCC 
CCACAGCCAC CCTCGGCCCT GGAGTCGGAT GGGGAAGGGC CGCCCCCCAG GGTGGGCTTT 
GTGGACAGCA CCATCAAGAG CCTGGACGAG AAGCTGCGGA CTCTGCTCTA CCAGGAGCAC 
GTGCCCACCT CCTCAGCCTC AGCTGGGACC CCTGTGGAGG TGGGCGACAG AGACTTCACC 
CTGGAGCCCC TGAGAGGGGA CCAGCCCCGC TCAGAGGTCT GCGGGGGGGA CCTGGCCCTG 
CCCCCAGTGC CTAAGGAGGC GGTCTCAGGG CGTGTCCAGC TGCCCCAGCC CTTGGTGGAG 
AAGTCAGAAC TGGCCCCCAC TCGAGGGGCC GTGATGGAGC AGGGCACGTC CTCGTCAATG 
ACAGCAGAGT CGTCTCCCAG GAGTATGCTA GGCTATGACA GAGATGGAAG GCAGGTGGCC 
TCAGACTCCC ATGTGGTCCC CAGCGTCCCC CAGGATGTAC CTGCTTTTGT GAGACCTGCA 
CGTGTGGAGC CCACAGACAG GGATGGTGGA GAAGCTGGAG AAAGCTCGGC AGAGCCCCCG 
CCGAGTGACA TGGGCACAGT GGGGGGCCAG GCTAGCCACC CCCAGACACT CGGCGCTCGA 
GCTTTGGGGT CCCCTCGGAA ACGTCCAGAG CAGCAGGATG TCAGCTCACC AGCCAAGACT 
GTGGGCCGTT TCTCGGTGGT CAGCACTCAG GACGAGTGGA CCCTGGCCTC CCCCCACAGC 
CTGAGATACT CTGCCCCACC CGACGTCTAC CTGGACGAGG CCCCCTCCAG CCCCGACGTG 
AAGCTGGCAG TGCGGCGGGC GCAGACGGCC TCCTCCATCG AGGTCGGCGT GGGCGAGCCC 
GTGTCCAGCG ACTCTGGGGA CGAGGGCCCT CGGGCGAGAC CCCCGGTGCA GAAGCAGGCG 
TCCCTGCCCG TGAGTGGCAG CGTGGCTGGC GACTTCGTGA AGAAGGCCAC CGCCTTCCTG 
CAGAGGCCTT CTCGGGCCGG CTCGCTGGGC CCCGAGACAC CCAGCAGGGT GGGCATGAAG 
GTCCCCACGA TCAGCGTGAC CTCCTTCCAT TCCCAGTCGT CCTACATCAG CAGCGACAAT 
GATTCGGAGC TCGAGGATGC TGACATAAAG AAGGAGCTGC AGAGTCTGCG GGAGAAGCAC 
CTGAAGGAGA TCTCGGAGCT GCAGAGCCAG CAGAAGCAGG AGATCGAAGC TCTGTACCGC 
CGCCTGGGCA AGCCACTGCC CCCCAACGTG GGCTTCTTCC ACACGGCACC CCCCACTGGC 
CGCCGGAGAA AAACCAGCAA GAGCAAGCTG AAGGCAGGCA AGCTGCTAAA TCCCCTGGTG 
CGGCAGCTCA AGGTCGTGGC CTCCAGCACA GGTCACTTGG CTGACTCCAG CAGAGGCCCT 
CCCGCTAAGG ACCCTGCCCA AGCCAGTGTG GGGCTCACTG CAGACAGCAC GGGCCTGAGC 
GGGAAGGCAG TGCAGACCCA GCAGCCCTGC TCCGTCCGGG CCTCCCTGTC TTCGGACATC 
TGCTCCGGCT TAGCCAGTGA TGGAGGCGGA GCGCGTGGCC AAGGCTGGAC GGTTTACCAC 
CCAACGTCTG AGAGAGTGAC CTATAAGTCT AGTAGCAAAC CTCGTGCTCG ATTCCTCAGT 
GGACCCGTAT CTGTGTCCAT CTGGTCTGCC CTGAAGCGTC TCTGCCTAGG CAAAGAACAC 
AGCAGTAGGT CCTCCACCAG CAGCCTGGCC CCAGGCCCTG AGCCAGGCCC CCAGCCCGCC 
CTGCACGTCC AGGCGCAGGT GAACAACAGC AACAACAAGA AGGGTACCTT CACGGACGAC 
CTGCACAAGC TGGTGGACGA GTGGACGAGC AAGACGGTGG GGGCCGCGCA GCTGAAGCCC 
ACGCTCAACC AGCTGAAGCA GACCCAGAAG CTGCAAGACA TGGAGGCCCA GGCAGGCTGG 
GCTGCCCCTG GCGAGGCGCG GGCTTCTGTG CGGTTGACTC CTTTTCCTCC CCGCCTGGAG 
ATCCCCGTGG TGTCGACTGG AAGCATGGAG GCACCTTGGG GAGATGACCG CACCTCGAGC 
AGGAGTGGGG ATGCCACGTC TGCCCCCAGC GCCCGGCCCT CTGTCCACCA CGGTCATTCC 
CGGAGCCGCC CCGACCCTGT CCGTGCCCAC ACCAGATGGC GCCCTCGGAA CCGCCCGGAG 
AAACCAGGTG TGGTTTGGCC TCCGAGTCCC CCCCACCGCC TGCTGTGGGC ACAGCACTCA 
GCCGCGAGGG GGACAGCGGG TGGGCAGCAA GACTGCTTCC TTTGCGGCTT CAGACCCTGT 
TCGCTCCTAG GTTCCTGTGG TCCACGCGCC GTCTCCACAC CCACTTCCTA TACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517241.1
CDS	318..6494
Translation	MLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_011518939.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518939.2

ORF Insert Sequence:

ATGCTGAAAG GCCTGCAGCA CCCCAACATC GTGCGCTTCT ACGACTTCTG GGAGTCCAGC 
GCCAAGGGCA AGCGGTGCAT TGTGCTGGTG ACGGAGCTGA TGACCTCAGG GACGCTGAAG 
ACATACCTGA AGCGGTTCAA GGTGATGAAG CCCAAGGTTC TCCGCAGCTG GTGCCGGCAG 
ATCCTGAAGG GCCTGCTGTT CCTGCACACA AGGACGCCAC CCATCATCCA CCGAGACCTG 
AAATGTGACA ATATTTTCAT CACCGGACCA ACTGGGTCTG TGAAGATTGG CGACTTGGGC 
CTGGCCACTC TGAAAAGAGC GTCATTTGCC AAAAGTGTGA TAGGTACTCC CGAGTTCATG 
GCGCCCGAGA TGTACGAGGA GCACTACGAT GAGTCCGTGG ACGTCTATGC CTTTGGGATG 
TGCATGCTGG AGATGGCCAC CTCGGAGTAC CCCTACTCGG AGTGCCAGAA TGCGGCCCAG 
ATCTACCGCA AGGTCACCTG TGGTATCAAG CCGGCCAGCT TTGAGAAAGT GCACGATCCT 
GAAATCAAGG AGATTATTGG GGAGTGTATC TGCAAAAACA AGGAGGAAAG GTACGAGATC 
AAAGACCTGC TGAGCCACGC CTTCTTCGCA GAGGACACAG GCGTGAGGGT GGAGCTCGCG 
GAGGAGGACC ACGGCAGGAA GTCCACCATC GCCCTGAGGC TCTGGGTGGA AGACCCCAAG 
AAACTGAAGG GAAAGCCCAA GGACAATGGA GCCATAGAGT TCACCTTCGA CCTGGAGAAG 
GAGACGCCGG ATGAGGTGGC CCAAGAGATG ATTGAGTCTG GATTCTTCCA CGAGAGTGAC 
GTCAAGATCG TGGCCAAGTC CATCCGTGAC CGCGTGGCCT TGATCCAGTG GCGGCGGGAG 
AGGATCTGGC CCGCGCTGCA GCCCAAGGAG CAGCAGGATG TGGGCAGCCC GGACAAGGCC 
AGGGGTCCGC CGGTGCCCCT GCAGGTCCAG GTGACCTACC ATGCACAGGC TGGGCAGCCC 
GGGCCACCAG AGCCCGAGGA GCCGGAGGCC GACCAGCACC TCCTGCCACC TACGTTGCCG 
ACCAGCGCCA CCTCCCTGGC CTCGGACAGC ACCTTCGACA GCGGCCAGGG CTCTACCGTG 
TACTCAGACT CGCAGAGCAG CCAGCAGAGC GTGATGCTTG GCTCCCTTGC CGACGCAGCG 
CCGTCCCCGG CCCAGTGTGT GTGCAGCCCC CCTGTGAGCG AGGGGCCCGT CCTGCCGCAG 
AGCCTGCCCT CGCTGGGGGC CTACCAGCAG CCCACGGCTG CACCTGGCTT GCCGGTGGGC 
TCTGTCCCGG CCCCCGCCTG CCCTCCGTCC CTCCAGCAGC ACTTCCCGGA TCCGGCCATG 
AGCTTCGCCC CCGTGCTGCC GCCGCCCAGC ACCCCCATGC CCACGGGCCC AGGCCAGCCA 
GCACCCCCCG GCCAGCAGCC TCCTCCGCTG GCCCAGCCGA CACCCCTGCC GCAGGTCCTG 
GCCCCACAGC CCGTGGTCCC CCTCCAGCCG GTTCCCCCCC ACCTGCCACC GTACCTGGCT 
CCAGCCTCCC AGGTGGGGGC CCCCGCTCAG CTGAAGCCCC TCCAGATGCC ACAGGCGCCC 
CTGCAGCCGC TTGCTCAAGT CCCTCCGCAG ATGCCCCCGA TTCCTGTTGT GCCCCCCATC 
ACGCCCCTGG CGGGAATCGA CGGCCTCCCT CCGGCCCTCC CAGACCTGCC GACCGCGACT 
GTGCCTCCCG TGCCACCACC TCAGTATTTC TCTCCAGCCG TGATCTTGCC GAGCCTCGCT 
GCCCCACTCC CCCCTGCGTC CCCAGCCTTG CCTCTGCAGG CTGTGAAGCT GCCCCACCCC 
CCTGGGGCGC CCCTGGCCAT GCCCTGCCGG ACCATTGTGC CAAATGCACC GGCCACTATC 
CCCCTGCTGG CCGTAGCCCC ACCGGGCGTG GCTGCCCTGT CCATTCATTC TGCCGTGGCC 
CAGCTCCCAG GCCAACCTGT GTACCCAGCG GCCTTCCCAC AGATGGCGCC TACTGACGTC 
CCTCCTTCCC CCCATCACAC GGTGCAGAAT ATGAGGGCCA CCCCTCCACA GCCGGCACTG 
CCTCCACAAC CCACACTGCC CCCACAACCC GTGCTGCCCC CGCAACCCAC GCTGCCCCCT 
CAACCTGTGT TGCCCCCGCA ACCCACACGG CCCCCTCAAC CTGTGCTGCC CCCGCAACCC 
ATGCTGCCCC CACAACCTGT GCTGCCCCCG CAGCCGGCAC TGCCTGTGCG CCCTGAGCCC 
CTCCAGCCCC ACCTTCCTGA ACAAGCTGCT CCAGCTGCTA CACCAGGGAG CCAGATTCTG 
CTTGGCCACC CAGCTCCCTA TGCTGTGGAC GTCGCCGCTC AGGTCCCCAC CGTGCCTGTG 
CCACCGGCTG CGGTCCTCTC GCCGCCTCTG CCGGAAGTGC TGCTGCCTGC CGCCCCTGAG 
CTCCTGCCTC AGTTCCCCAG CTCCCTGGCC ACGGTGTCTG CCTCTGTGCA GAGTGTGCCC 
ACCCAGACTG CCACACTTCT GCCACCAGCA AACCCACCGC TGCCTGGCGG GCCCGGGATC 
GCCAGCCCTT GCCCAACTGT CCAGCTGACG GTGGAACCAG TCCAAGAGGA GCAGGCCTCA 
CAGGACAAGC CGCCCGGCCT CCCGCAGAGC TGTGAGAGCT ATGGAGGTTC TGATGTCACT 
TCTGGAAAAG AGCTGAGTGA CAGCTGTGAA GGCGCCTTTG GAGGGGGCAG GCTGGAGGGC 
AGGGCAGCCC GAAAACACCA CCGCAGGTCC ACGCGTGCGC GCTCCCGGCA GGAGAGGGCC 
AGCCGGCCCC GGCTTACCAT CTTGAACGTG TGCAACACTG GGGACAAGAT GGTGGAGTGC 
CAGCTGGAGA CGCACAACCA CAAGATGGTG ACCTTCAAGT TCGACTTGGA CGGGGACGCA 
CCCGATGAAA TTGCCACGTA TATGGTGGAG CATGACTTTA TCCTGCAGGC CGAGCGGGAA 
ACGTTCATCG AGCAGATGAA GGATGTCATG GACAAGGCAG AGGACATGCT CAGCGAGGAC 
ACAGACGCCG ACCGTGGCTC CGACCCAGGG ACCAGCCCGC CACACCTCAG CACCTGCGGC 
CTGGGCACCG GGGAGGAGAG CCGACAATCC CAAGCCAACG CCCCCGTGTA TCAGCAGAAC 
GTCCTGCACA CCGGGAAGAG GTGGTTCATC ATCTGTCCGG TGGCTGAGCA CCCCGCCCCC 
GAGGCCCCTG AATCTTCGCC CCCACTTCCT CTAAGCTCCC TGCCGCCAGA AGCCAGCCAA 
GATTCAGCGC CCTATAAAGA CCAGCTGTCC TCGAAGGAAC AACCCAGCTT TCTAGCCAGT 
CAGCAGCTCC TGAGCCAGGC GGGCCCCAGC AACCCTCCTG GGGCACCCCC AGCCCCTTTG 
GCCCCCTCCT CCCCTCCTGT GACTGCTCTG CCCCAAGATG GAGCAGCTCC AGCCACCAGC 
ACCATGCCAG AGCCAGCGTC AGGAACTGCC AGCCAGGCAG GGGGTCCAGG GACACCTCAG 
GGGCTGACCA GTGAGCTCGA GACGTCTCAG CCACTAGCGG AGACTCACGA GGCCCCGCTT 
GCTGTGCAGC CCCTCGTGGT GGGCCTAGCA CCTTGCACTC CAGCTCCAGA GGCTGCCTCA 
ACCAGGGACG CCAGTGCCCC AAGGGAGCCC CTGCCACCTC CTGCACCTGA GCCCAGCCCC 
CACAGCGGGA CCCCACAGCC CGCCTTGGGT CAACCTGCTC CCCTGCTTCC TGCCGCAGTG 
GGGGCCGTCA GCCTGGCCAC CTCCCAGCTC CCAAGCCCAC CCCTGGGGCC CACCGTCCCC 
CCACAGCCAC CCTCGGCCCT GGAGTCGGAT GGGGAAGGGC CGCCCCCCAG GGTGGGCTTT 
GTGGACAGCA CCATCAAGAG CCTGGACGAG AAGCTGCGGA CTCTGCTCTA CCAGGAGCAC 
GTGCCCACCT CCTCAGCCTC AGCTGGGACC CCTGTGGAGG TGGGCGACAG AGACTTCACC 
CTGGAGCCCC TGAGAGGGGA CCAGCCCCGC TCAGAGGTCT GCGGGGGGGA CCTGGCCCTG 
CCCCCAGTGC CTAAGGAGGC GGTCTCAGGG CGTGTCCAGC TGCCCCAGCC CTTGGTGGAG 
AAGTCAGAAC TGGCCCCCAC TCGAGGGGCC GTGATGGAGC AGGGCACGTC CTCGTCAATG 
ACAGCAGAGT CGTCTCCCAG GAGTATGCTA GGCTATGACA GAGATGGAAG GCAGGTGGCC 
TCAGACTCCC ATGTGGTCCC CAGCGTCCCC CAGGATGTAC CTGCTTTTGT GAGACCTGCA 
CGTGTGGAGC CCACAGACAG GGATGGTGGA GAAGCTGGAG AAAGCTCGGC AGAGCCCCCG 
CCGAGTGACA TGGGCACAGT GGGGGGCCAG GCTAGCCACC CCCAGACACT CGGCGCTCGA 
GCTTTGGGGT CCCCTCGGAA ACGTCCAGAG CAGCAGGATG TCAGCTCACC AGCCAAGACT 
GTGGGCCGTT TCTCGGTGGT CAGCACTCAG GACGAGTGGA CCCTGGCCTC CCCCCACAGC 
CTGAGATACT CTGCCCCACC CGACGTCTAC CTGGACGAGG CCCCCTCCAG CCCCGACGTG 
AAGCTGGCAG TGCGGCGGGC GCAGACGGCC TCCTCCATCG AGGTCGGCGT GGGCGAGCCC 
GTGTCCAGCG ACTCTGGGGA CGAGGGCCCT CGGGCGAGAC CCCCGGTGCA GAAGCAGGCG 
TCCCTGCCCG TGAGTGGCAG CGTGGCTGGC GACTTCGTGA AGAAGGCCAC CGCCTTCCTG 
CAGAGGCCTT CTCGGGCCGG CTCGCTGGGC CCCGAGACAC CCAGCAGGGT GGGCATGAAG 
GTCCCCACGA TCAGCGTGAC CTCCTTCCAT TCCCAGTCGT CCTACATCAG CAGCGACAAT 
GATTCGGAGC TCGAGGATGC TGACATAAAG AAGGAGCTGC AGAGTCTGCG GGAGAAGCAC 
CTGAAGGAGA TCTCGGAGCT GCAGAGCCAG CAGAAGCAGG AGATCGAAGC TCTGTACCGC 
CGCCTGGGCA AGCCACTGCC CCCCAACGTG GGCTTCTTCC ACACGGCACC CCCCACTGGC 
CGCCGGAGAA AAACCAGCAA GAGCAAGCTG AAGGCAGGCA AGCTGCTAAA TCCCCTGGTG 
CGGCAGCTCA AGGTCGTGGC CTCCAGCACA GGTCACTTGG CTGACTCCAG CAGAGGCCCT 
CCCGCTAAGG ACCCTGCCCA AGCCAGTGTG GGGCTCACTG CAGACAGCAC GGGCCTGAGC 
GGGAAGGCAG TGCAGACCCA GCAGCCCTGC TCCGTCCGGG CCTCCCTGTC TTCGGACATC 
TGCTCCGGCT TAGCCAGTGA TGGAGGCGGA GCGCGTGGCC AAGGCTGGAC GGTTTACCAC 
CCAACGTCTG AGAGAGTGAC CTATAAGTCT AGTAGCAAAC CTCGTGCTCG ATTCCTCAGT 
GGACCCGTAT CTGTGTCCAT CTGGTCTGCC CTGAAGCGTC TCTGCCTAGG CAAAGAACAC 
AGCAGTAGGT CCTCCACCAG CAGCCTGGCC CCAGGCCCTG AGCCAGGCCC CCAGCCCGCC 
CTGCACGTCC AGGCGCAGGT GAACAACAGC AACAACAAGA AGGGTACCTT CACGGACGAC 
CTGCACAAGC TGGTGGACGA GTGGACGAGC AAGACGGTGG GGGCCGCGCA GCTGAAGCCC 
ACGCTCAACC AGCTGAAGCA GACCCAGAAG CTGCAAGACA TGGAGGCCCA GGCAGGCTGG 
GCTGCCCCTG GCGAGGCGCG GGCTTCTGTG CGGTTGACTC CTTTTCCTCC CCGCCTGGAG 
ATCCCCGTGG TGTCGACTGG AAGCATGGAG GCACCTTGGG GAGATGACCG CACCTCGAGC 
AGGAGTGGGG ATGCCACGTC TGCCCCCAGC GCCCGGCCCT CTGTCCACCA CGGTCATTCC 
CGGAGCCGCC CCGACCCTGT CCGTGCCCAC ACCAGATGGC GCCCTCGGAA CCGCCCGGAG 
AAACCAGGTG TGGTTTGGCC TCCGAGTCCC CCCCACCGCC TGCTGTGGGC ACAGCACTCA 
GCCGCGAGGG GGACAGCGGG TGGGCAGCAA GACTGCTTCC TTTGCGGCTT CAGACCCTGT 
TCGCTCCTAG GTTCCTGTGG TCCACGCGCC GTCTCCACAC CCACTTCCTA TACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_017015045.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870534.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA.

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CloneID	OHu98844
Clone ID Related Accession (Same CDS sequence)	XM_017015045.1
Accession Version	XM_017015045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGCAA GCTGCTAAAT 
CCCCTGGTGC GGCAGCTCAA GGTCGTGGCC TCCAGCACAG GTCACTTGGC TGACTCCAGC 
AGAGGCCCTC CCGCTAAGGA CCCTGCCCAA GCCAGTGTGG GGCTCACTGC AGACAGCACG 
GGCCTGAGCG GGAAGGCAGT GCAGACCCAG CAGCCCTGCT CCGTCCGGGC CTCCCTGTCT 
TCGGACATCT GCTCCGGCTT AGCCAGTGAT GGAGGCGGAG CGCGTGGCCA AGGCTGGACG 
GTTTACCACC CAACGTCTGA GAGAGTGACC TATAAGTCTA GTAGCAAACC TCGTGCTCGA 
TTCCTCAGTG GACCCGTATC TGTGTCCATC TGGTCTGCCC TGAAGCGTCT CTGCCTAGGC 
AAAGAACACA GCAGTAGGTC CTCCACCAGC AGCCTGGCCC CAGGCCCTGA GCCAGGCCCC 
CAGCCCGCCC TGCACGTCCA GGCGCAGGTG AACAACAGCA ACAACAAGAA GGGTACCTTC 
ACGGACGACC TGCACAAGCT GGTGGACGAG TGGACGAGCA AGACGGTGGG GGCCGCGCAG 
CTGAAGCCCA CGCTCAACCA GCTGAAGCAG ACCCAGAAGC TGCAAGACAT GGAGGCCCAG 
GCAGGCTGGG CTGCCCCTGG CGAGGCGCGG GCTATGACCG CACCTCGAGC AGGAGTGGGG 
ATGCCACGTC TGCCCCCAGC GCCCGGCCCT CTGTCCACCA CGGTCATTCC CGGAGCCGCC 
CCGACCCTGT CCGTGCCCAC ACCAGATGGC GCCCTCGGAA CCGCCCGGAG AAACCAGGTG 
TGGTTTGGCC TCCGAGTCCC CCCCACCGCC TGCTGTGGGC ACAGCACTCA GCCGCGAGGG 
GGACAGCGGG TGGGCAGCAA GACTGCTTCC TTTGCGGCTT CAGACCCTGT TCGCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870534.1
CDS	233..7012
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	XM_017015045.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015045.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGCAA GCTGCTAAAT 
CCCCTGGTGC GGCAGCTCAA GGTCGTGGCC TCCAGCACAG GTCACTTGGC TGACTCCAGC 
AGAGGCCCTC CCGCTAAGGA CCCTGCCCAA GCCAGTGTGG GGCTCACTGC AGACAGCACG 
GGCCTGAGCG GGAAGGCAGT GCAGACCCAG CAGCCCTGCT CCGTCCGGGC CTCCCTGTCT 
TCGGACATCT GCTCCGGCTT AGCCAGTGAT GGAGGCGGAG CGCGTGGCCA AGGCTGGACG 
GTTTACCACC CAACGTCTGA GAGAGTGACC TATAAGTCTA GTAGCAAACC TCGTGCTCGA 
TTCCTCAGTG GACCCGTATC TGTGTCCATC TGGTCTGCCC TGAAGCGTCT CTGCCTAGGC 
AAAGAACACA GCAGTAGGTC CTCCACCAGC AGCCTGGCCC CAGGCCCTGA GCCAGGCCCC 
CAGCCCGCCC TGCACGTCCA GGCGCAGGTG AACAACAGCA ACAACAAGAA GGGTACCTTC 
ACGGACGACC TGCACAAGCT GGTGGACGAG TGGACGAGCA AGACGGTGGG GGCCGCGCAG 
CTGAAGCCCA CGCTCAACCA GCTGAAGCAG ACCCAGAAGC TGCAAGACAT GGAGGCCCAG 
GCAGGCTGGG CTGCCCCTGG CGAGGCGCGG GCTATGACCG CACCTCGAGC AGGAGTGGGG 
ATGCCACGTC TGCCCCCAGC GCCCGGCCCT CTGTCCACCA CGGTCATTCC CGGAGCCGCC 
CCGACCCTGT CCGTGCCCAC ACCAGATGGC GCCCTCGGAA CCGCCCGGAG AAACCAGGTG 
TGGTTTGGCC TCCGAGTCCC CCCCACCGCC TGCTGTGGGC ACAGCACTCA GCCGCGAGGG 
GGACAGCGGG TGGGCAGCAA GACTGCTTCC TTTGCGGCTT CAGACCCTGT TCGCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_017015046.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870535.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X8, mRNA.

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CloneID	OHu98845
Clone ID Related Accession (Same CDS sequence)	XM_017015046.1
Accession Version	XM_017015046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGAGAAG 
TCAGAACTGG CCCCCACTCG AGGGGCCGTG ATGGAGCAGG GCACGTCCTC GTCAATGACA 
GAGTCGTCTC CCAGGAGTAT GCTAGGCTAT GACAGAGATG GAAGGCAGGT GGCCTCAGAC 
TCCCATGTGG TCCCCAGCGT CCCCCAGGAT GTACCTGCTT TTGTGAGACC TGCACGTGTG 
GAGCCCACAG ACAGGGATGG TGGAGAAGCT GGAGAAAGCT CGGCAGAGCC CCCGCCGAGT 
GACATGGGCA CAGTGGGGGG CCAGGCTAGC CACCCCCAGA CACTCGGCGC TCGAGCTTTG 
GGGTCCCCTC GGAAACGTCC AGAGCAGCAG GATGTCAGCT CACCAGCCAA GACTGTGGGC 
CGTTTCTCGG TGGTCAGCAC TCAGGACGAG TGGACCCTGG CCTCCCCCCA CAGCCTGAGA 
TACTCTGCCC CACCCGACGT CTACCTGGAC GAGGCCCCCT CCAGCCCCGA CGTGAAGCTG 
GCAGTGCGGC GGGCGCAGAC GGCCTCCTCC ATCGAGGTCG GCGTGGGCGA GCCCGTGTCC 
AGCGACTCTG GGGACGAGGG CCCTCGGGCG AGACCCCCGG TGCAGAAGCA GGCGTCCCTG 
CCCGTGAGTG GCAGCGTGGC TGGCGACTTC GTGAAGAAGG CCACCGCCTT CCTGCAGAGG 
CCTTCTCGGG CCGGCTCGCT GGGCCCCGAG ACACCCAGCA GGGTGGGCAT GAAGGTCCCC 
ACGATCAGCG TGACCTCCTT CCATTCCCAG TCGTCCTACA TCAGCAGCGA CAATGATTCG 
GAGCTCGAGG ATGCTGACAT AAAGAAGGAG CTGCAGAGTC TGCGGGAGAA GCACCTGAAG 
GAGATCTCGG AGCTGCAGAG CCAGCAGAAG CAGGAGATCG AAGCTCTGTA CCGCCGCCTG 
GGCAAGCCAC TGCCCCCCAA CGTGGGCTTC TTCCACACGG CACCCCCCAC TGGCCGCCGG 
AGAAAAACCA GCAAGAGCAA GCTGAAGGCA GGCAAGCTGC TAAATCCCCT GGTGCGGCAG 
CTCAAGGTCG TGGCCTCCAG CACAGGTCAC TTGGCTGACT CCAGCAGAGG CCCTCCCGCT 
AAGGACCCTG CCCAAGCCAG TGTGGGGCTC ACTGCAGACA GCACGGGCCT GAGCGGGAAG 
GCAGTGCAGA CCCAGCAGCC CTGCTCCGTC CGGGCCTCCC TGTCTTCGGA CATCTGCTCC 
GGCTTAGCCA GTGATGGAGG CGGAGCGCGT GGCCAAGGCT GGACGGTTTA CCACCCAACG 
TCTGAGAGAG TGACCTATAA GTCTAGTAGC AAACCTCGTG CTCGATTCCT CAGTGGACCC 
GTATCTGTGT CCATCTGGTC TGCCCTGAAG CGTCTCTGCC TAGGCAAAGA ACACAGCAGT 
AGGTCCTCCA CCAGCAGCCT GGCCCCAGGC CCTGAGCCAG GCCCCCAGCC CGCCCTGCAC 
GTCCAGGCGC AGGTGAACAA CAGCAACAAC AAGAAGGGTA CCTTCACGGA CGACCTGCAC 
AAGCTGGTGG ACGAGTGGAC GAGCAAGACG GTGGGGGCCG CGCAGCTGAA GCCCACGCTC 
AACCAGCTGA AGCAGACCCA GAAGCTGCAA GACATGGAGG CCCAGGCAGG CTGGGCTGCC 
CCTGGCGAGG CGCGGGCTTC TGTGCGGTTG ACTCCTTTTC CTCCCCGCCT GGAGATCCCC 
GTGGTGTCGA CTGGAAGCAT GGAGGCACCT TGGGGAGATG ACCGCACCTC GAGCAGGAGT 
GGGGATGCCA CGTCTGCCCC CAGCGCCCGG CCCTCTGTCC ACCACGGTCA TTCCCGGAGC 
CGCCCCGACC CTGTCCGTGC CCACACCAGA TGGCGCCCTC GGAACCGCCC GGAGAAACCA 
GGTGTGGTTT GGCCTCCGAG TCCCCCCCAC CGCCTGCTGT GGGCACAGCA CTCAGCCGCG 
AGGGGGACAG CGGGTGGGCA GCAAGACTGC TTCCTTTGCG GCTTCAGACC CTGTTCGCTC 
CTAGGTTCCT GTGGTCCACG CGCCGTCTCC ACACCCACTT CCTATACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870535.1
CDS	233..7003
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVEKSELAPTRGAVMEQGTSSSMTESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARASVRLTPFPPRLEIPVVSTGSMEAPWGDDRTSSRSGDATSAPSARPSVHHGHSRSRPDPVRAHTRWRPRNRPEKPGVVWPPSPPHRLLWAQHSAARGTAGGQQDCFLCGFRPCSLLGSCGPRAVSTPTSYT

Target ORF information:

RefSeq Version	XM_017015046.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015046.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGAGAAG 
TCAGAACTGG CCCCCACTCG AGGGGCCGTG ATGGAGCAGG GCACGTCCTC GTCAATGACA 
GAGTCGTCTC CCAGGAGTAT GCTAGGCTAT GACAGAGATG GAAGGCAGGT GGCCTCAGAC 
TCCCATGTGG TCCCCAGCGT CCCCCAGGAT GTACCTGCTT TTGTGAGACC TGCACGTGTG 
GAGCCCACAG ACAGGGATGG TGGAGAAGCT GGAGAAAGCT CGGCAGAGCC CCCGCCGAGT 
GACATGGGCA CAGTGGGGGG CCAGGCTAGC CACCCCCAGA CACTCGGCGC TCGAGCTTTG 
GGGTCCCCTC GGAAACGTCC AGAGCAGCAG GATGTCAGCT CACCAGCCAA GACTGTGGGC 
CGTTTCTCGG TGGTCAGCAC TCAGGACGAG TGGACCCTGG CCTCCCCCCA CAGCCTGAGA 
TACTCTGCCC CACCCGACGT CTACCTGGAC GAGGCCCCCT CCAGCCCCGA CGTGAAGCTG 
GCAGTGCGGC GGGCGCAGAC GGCCTCCTCC ATCGAGGTCG GCGTGGGCGA GCCCGTGTCC 
AGCGACTCTG GGGACGAGGG CCCTCGGGCG AGACCCCCGG TGCAGAAGCA GGCGTCCCTG 
CCCGTGAGTG GCAGCGTGGC TGGCGACTTC GTGAAGAAGG CCACCGCCTT CCTGCAGAGG 
CCTTCTCGGG CCGGCTCGCT GGGCCCCGAG ACACCCAGCA GGGTGGGCAT GAAGGTCCCC 
ACGATCAGCG TGACCTCCTT CCATTCCCAG TCGTCCTACA TCAGCAGCGA CAATGATTCG 
GAGCTCGAGG ATGCTGACAT AAAGAAGGAG CTGCAGAGTC TGCGGGAGAA GCACCTGAAG 
GAGATCTCGG AGCTGCAGAG CCAGCAGAAG CAGGAGATCG AAGCTCTGTA CCGCCGCCTG 
GGCAAGCCAC TGCCCCCCAA CGTGGGCTTC TTCCACACGG CACCCCCCAC TGGCCGCCGG 
AGAAAAACCA GCAAGAGCAA GCTGAAGGCA GGCAAGCTGC TAAATCCCCT GGTGCGGCAG 
CTCAAGGTCG TGGCCTCCAG CACAGGTCAC TTGGCTGACT CCAGCAGAGG CCCTCCCGCT 
AAGGACCCTG CCCAAGCCAG TGTGGGGCTC ACTGCAGACA GCACGGGCCT GAGCGGGAAG 
GCAGTGCAGA CCCAGCAGCC CTGCTCCGTC CGGGCCTCCC TGTCTTCGGA CATCTGCTCC 
GGCTTAGCCA GTGATGGAGG CGGAGCGCGT GGCCAAGGCT GGACGGTTTA CCACCCAACG 
TCTGAGAGAG TGACCTATAA GTCTAGTAGC AAACCTCGTG CTCGATTCCT CAGTGGACCC 
GTATCTGTGT CCATCTGGTC TGCCCTGAAG CGTCTCTGCC TAGGCAAAGA ACACAGCAGT 
AGGTCCTCCA CCAGCAGCCT GGCCCCAGGC CCTGAGCCAG GCCCCCAGCC CGCCCTGCAC 
GTCCAGGCGC AGGTGAACAA CAGCAACAAC AAGAAGGGTA CCTTCACGGA CGACCTGCAC 
AAGCTGGTGG ACGAGTGGAC GAGCAAGACG GTGGGGGCCG CGCAGCTGAA GCCCACGCTC 
AACCAGCTGA AGCAGACCCA GAAGCTGCAA GACATGGAGG CCCAGGCAGG CTGGGCTGCC 
CCTGGCGAGG CGCGGGCTTC TGTGCGGTTG ACTCCTTTTC CTCCCCGCCT GGAGATCCCC 
GTGGTGTCGA CTGGAAGCAT GGAGGCACCT TGGGGAGATG ACCGCACCTC GAGCAGGAGT 
GGGGATGCCA CGTCTGCCCC CAGCGCCCGG CCCTCTGTCC ACCACGGTCA TTCCCGGAGC 
CGCCCCGACC CTGTCCGTGC CCACACCAGA TGGCGCCCTC GGAACCGCCC GGAGAAACCA 
GGTGTGGTTT GGCCTCCGAG TCCCCCCCAC CGCCTGCTGT GGGCACAGCA CTCAGCCGCG 
AGGGGGACAG CGGGTGGGCA GCAAGACTGC TTCCTTTGCG GCTTCAGACC CTGTTCGCTC 
CTAGGTTCCT GTGGTCCACG CGCCGTCTCC ACACCCACTT CCTATACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_017015047.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870536.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X11, mRNA.

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CloneID	OHu98846
Clone ID Related Accession (Same CDS sequence)	XM_017015047.1
Accession Version	XM_017015047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG TCACTTGGCT 
GACTCCAGCA GAGGCCCTCC CGCTAAGGAC CCTGCCCAAG CCAGTGTGGG GCTCACTGCA 
GACAGCACGG GCCTGAGCGG GAAGGCAGTG CAGACCCAGC AGCCCTGCTC CGTCCGGGCC 
TCCCTGTCTT CGGACATCTG CTCCGGCTTA GCCAGTGATG GAGGCGGAGC GCGTGGCCAA 
GGCTGGACGG TTTACCACCC AACGTCTGAG AGAGTGACCT ATAAGTCTAG TAGCAAACCT 
CGTGCTCGAT TCCTCAGTGG ACCCGTATCT GTGTCCATCT GGTCTGCCCT GAAGCGTCTC 
TGCCTAGGCA AAGAACACAG CAGTAGGTCC TCCACCAGCA GCCTGGCCCC AGGCCCTGAG 
CCAGGCCCCC AGCCCGCCCT GCACGTCCAG GCGCAGGTGA ACAACAGCAA CAACAAGAAG 
GGTACCTTCA CGGACGACCT GCACAAGCTG GTGGACGAGT GGACGAGCAA GACGGTGGGG 
GCCGCGCAGC TGAAGCCCAC GCTCAACCAG CTGAAGCAGA CCCAGAAGCT GCAAGACATG 
GAGGCCCAGG CAGGCTGGGC TGCCCCTGGC GAGGCGCGGG CTATGACCGC ACCTCGAGCA 
GGAGTGGGGA TGCCACGTCT GCCCCCAGCG CCCGGCCCTC TGTCCACCAC GGTCATTCCC 
GGAGCCGCCC CGACCCTGTC CGTGCCCACA CCAGATGGCG CCCTCGGAAC CGCCCGGAGA 
AACCAGGTGT GGTTTGGCCT CCGAGTCCCC CCCACCGCCT GCTGTGGGCA CAGCACTCAG 
CCGCGAGGGG GACAGCGGGT GGGCAGCAAG ACTGCTTCCT TTGCGGCTTC AGACCCTGTT 
CGCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870536.1
CDS	233..6961
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	XM_017015047.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015047.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG TCACTTGGCT 
GACTCCAGCA GAGGCCCTCC CGCTAAGGAC CCTGCCCAAG CCAGTGTGGG GCTCACTGCA 
GACAGCACGG GCCTGAGCGG GAAGGCAGTG CAGACCCAGC AGCCCTGCTC CGTCCGGGCC 
TCCCTGTCTT CGGACATCTG CTCCGGCTTA GCCAGTGATG GAGGCGGAGC GCGTGGCCAA 
GGCTGGACGG TTTACCACCC AACGTCTGAG AGAGTGACCT ATAAGTCTAG TAGCAAACCT 
CGTGCTCGAT TCCTCAGTGG ACCCGTATCT GTGTCCATCT GGTCTGCCCT GAAGCGTCTC 
TGCCTAGGCA AAGAACACAG CAGTAGGTCC TCCACCAGCA GCCTGGCCCC AGGCCCTGAG 
CCAGGCCCCC AGCCCGCCCT GCACGTCCAG GCGCAGGTGA ACAACAGCAA CAACAAGAAG 
GGTACCTTCA CGGACGACCT GCACAAGCTG GTGGACGAGT GGACGAGCAA GACGGTGGGG 
GCCGCGCAGC TGAAGCCCAC GCTCAACCAG CTGAAGCAGA CCCAGAAGCT GCAAGACATG 
GAGGCCCAGG CAGGCTGGGC TGCCCCTGGC GAGGCGCGGG CTATGACCGC ACCTCGAGCA 
GGAGTGGGGA TGCCACGTCT GCCCCCAGCG CCCGGCCCTC TGTCCACCAC GGTCATTCCC 
GGAGCCGCCC CGACCCTGTC CGTGCCCACA CCAGATGGCG CCCTCGGAAC CGCCCGGAGA 
AACCAGGTGT GGTTTGGCCT CCGAGTCCCC CCCACCGCCT GCTGTGGGCA CAGCACTCAG 
CCGCGAGGGG GACAGCGGGT GGGCAGCAAG ACTGCTTCCT TTGCGGCTTC AGACCCTGTT 
CGCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_017015048.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870537.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X12, mRNA.

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CloneID	OHu98847
Clone ID Related Accession (Same CDS sequence)	XM_017015048.1
Accession Version	XM_017015048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGTCA CTTGGCTGAC 
TCCAGCAGAG GCCCTCCCGC TAAGGACCCT GCCCAAGCCA GTGTGGGGCT CACTGCAGAC 
AGCACGGGCC TGAGCGGGAA GGCAGTGCAG ACCCAGCAGC CCTGCTCCGT CCGGGCCTCC 
CTGTCTTCGG ACATCTGCTC CGGCTTAGCC AGTGATGGAG GCGGAGCGCG TGGCCAAGGC 
TGGACGGTTT ACCACCCAAC GTCTGAGAGA GTGACCTATA AGTCTAGTAG CAAACCTCGT 
GCTCGATTCC TCAGTGGACC CGTATCTGTG TCCATCTGGT CTGCCCTGAA GCGTCTCTGC 
CTAGGCAAAG AACACAGCAG TAGGTCCTCC ACCAGCAGCC TGGCCCCAGG CCCTGAGCCA 
GGCCCCCAGC CCGCCCTGCA CGTCCAGGCG CAGGTGAACA ACAGCAACAA CAAGAAGGGT 
ACCTTCACGG ACGACCTGCA CAAGCTGGTG GACGAGTGGA CGAGCAAGAC GGTGGGGGCC 
GCGCAGCTGA AGCCCACGCT CAACCAGCTG AAGCAGACCC AGAAGCTGCA AGACATGGAG 
GCCCAGGCAG GCTGGGCTGC CCCTGGCGAG GCGCGGGCTA TGACCGCACC TCGAGCAGGA 
GTGGGGATGC CACGTCTGCC CCCAGCGCCC GGCCCTCTGT CCACCACGGT CATTCCCGGA 
GCCGCCCCGA CCCTGTCCGT GCCCACACCA GATGGCGCCC TCGGAACCGC CCGGAGAAAC 
CAGGTGTGGT TTGGCCTCCG AGTCCCCCCC ACCGCCTGCT GTGGGCACAG CACTCAGCCG 
CGAGGGGGAC AGCGGGTGGG CAGCAAGACT GCTTCCTTTG CGGCTTCAGA CCCTGTTCGC 
TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870537.1
CDS	233..6958
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	XM_017015048.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015048.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGTCA CTTGGCTGAC 
TCCAGCAGAG GCCCTCCCGC TAAGGACCCT GCCCAAGCCA GTGTGGGGCT CACTGCAGAC 
AGCACGGGCC TGAGCGGGAA GGCAGTGCAG ACCCAGCAGC CCTGCTCCGT CCGGGCCTCC 
CTGTCTTCGG ACATCTGCTC CGGCTTAGCC AGTGATGGAG GCGGAGCGCG TGGCCAAGGC 
TGGACGGTTT ACCACCCAAC GTCTGAGAGA GTGACCTATA AGTCTAGTAG CAAACCTCGT 
GCTCGATTCC TCAGTGGACC CGTATCTGTG TCCATCTGGT CTGCCCTGAA GCGTCTCTGC 
CTAGGCAAAG AACACAGCAG TAGGTCCTCC ACCAGCAGCC TGGCCCCAGG CCCTGAGCCA 
GGCCCCCAGC CCGCCCTGCA CGTCCAGGCG CAGGTGAACA ACAGCAACAA CAAGAAGGGT 
ACCTTCACGG ACGACCTGCA CAAGCTGGTG GACGAGTGGA CGAGCAAGAC GGTGGGGGCC 
GCGCAGCTGA AGCCCACGCT CAACCAGCTG AAGCAGACCC AGAAGCTGCA AGACATGGAG 
GCCCAGGCAG GCTGGGCTGC CCCTGGCGAG GCGCGGGCTA TGACCGCACC TCGAGCAGGA 
GTGGGGATGC CACGTCTGCC CCCAGCGCCC GGCCCTCTGT CCACCACGGT CATTCCCGGA 
GCCGCCCCGA CCCTGTCCGT GCCCACACCA GATGGCGCCC TCGGAACCGC CCGGAGAAAC 
CAGGTGTGGT TTGGCCTCCG AGTCCCCCCC ACCGCCTGCT GTGGGCACAG CACTCAGCCG 
CGAGGGGGAC AGCGGGTGGG CAGCAAGACT GCTTCCTTTG CGGCTTCAGA CCCTGTTCGC 
TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_017015049.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870538.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X14, mRNA.

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CloneID	OHu98848
Clone ID Related Accession (Same CDS sequence)	XM_017015049.1
Accession Version	XM_017015049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGGTCT 
GCCCTGAAGC GTCTCTGCCT AGGCAAAGAA CACAGCAGTA GGTCCTCCAC CAGCAGCCTG 
GCCCCAGGCC CTGAGCCAGG CCCCCAGCCC GCCCTGCACG TCCAGGCGCA GGTGAACAAC 
AGCAACAACA AGAAGGGTAC CTTCACGGAC GACCTGCACA AGCTGGTGGA CGAGTGGACG 
AGCAAGACGG TGGGGGCCGC GCAGCTGAAG CCCACGCTCA ACCAGCTGAA GCAGACCCAG 
AAGCTGCAAG ACATGGAGGC CCAGGCAGGC TGGGCTGCCC CTGGCGAGGC GCGGGCTATG 
ACCGCACCTC GAGCAGGAGT GGGGATGCCA CGTCTGCCCC CAGCGCCCGG CCCTCTGTCC 
ACCACGGTCA TTCCCGGAGC CGCCCCGACC CTGTCCGTGC CCACACCAGA TGGCGCCCTC 
GGAACCGCCC GGAGAAACCA GGTGTGGTTT GGCCTCCGAG TCCCCCCCAC CGCCTGCTGT 
GGGCACAGCA CTCAGCCGCG AGGGGGACAG CGGGTGGGCA GCAAGACTGC TTCCTTTGCG 
GCTTCAGACC CTGTTCGCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870538.1
CDS	233..6916
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	XM_017015049.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015049.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGTCACTT GGCTGACTCC 
AGCAGAGGCC CTCCCGCTAA GGACCCTGCC CAAGCCAGTG TGGGGCTCAC TGCAGACAGC 
ACGGGCCTGA GCGGGAAGGC AGTGCAGACC CAGCAGCCCT GCTCCGTCCG GGCCTCCCTG 
TCTTCGGACA TCTGCTCCGG CTTAGCCAGT GATGGAGGCG GAGCGCGTGG CCAAGGGTCT 
GCCCTGAAGC GTCTCTGCCT AGGCAAAGAA CACAGCAGTA GGTCCTCCAC CAGCAGCCTG 
GCCCCAGGCC CTGAGCCAGG CCCCCAGCCC GCCCTGCACG TCCAGGCGCA GGTGAACAAC 
AGCAACAACA AGAAGGGTAC CTTCACGGAC GACCTGCACA AGCTGGTGGA CGAGTGGACG 
AGCAAGACGG TGGGGGCCGC GCAGCTGAAG CCCACGCTCA ACCAGCTGAA GCAGACCCAG 
AAGCTGCAAG ACATGGAGGC CCAGGCAGGC TGGGCTGCCC CTGGCGAGGC GCGGGCTATG 
ACCGCACCTC GAGCAGGAGT GGGGATGCCA CGTCTGCCCC CAGCGCCCGG CCCTCTGTCC 
ACCACGGTCA TTCCCGGAGC CGCCCCGACC CTGTCCGTGC CCACACCAGA TGGCGCCCTC 
GGAACCGCCC GGAGAAACCA GGTGTGGTTT GGCCTCCGAG TCCCCCCCAC CGCCTGCTGT 
GGGCACAGCA CTCAGCCGCG AGGGGGACAG CGGGTGGGCA GCAAGACTGC TTCCTTTGCG 
GCTTCAGACC CTGTTCGCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_017015050.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870539.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X15, mRNA.

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CloneID	OHu98849
Clone ID Related Accession (Same CDS sequence)	XM_017015050.1
Accession Version	XM_017015050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGCAA GCTGCTAAAT 
CCCCTGGTGC GGCAGCTCAA GGTCGTGGCC TCCAGCACAG GTCACTTGGC TGACTCCAGC 
AGAGGCCCTC CCGCTAAGGA CCCTGCCCAA GCCAGTGTGG GGCTCACTGC AGACAGCACG 
GGCCTGAGCG GGAAGGCAGT GCAGACCCAG CAGCCCTGCT CCGTCCGGGC CTCCCTGTCT 
TCGGACATCT GCTCCGGCTT AGCCAGTGAT GGAGGCGGAG CGCGTGGCCA AGGGTCTGCC 
CTGAAGCGTC TCTGCCTAGG CAAAGAACAC AGCAGTAGGT CCTCCACCAG CAGCCTGGCC 
CCAGGCCCTG AGCCAGGCCC CCAGCCCGCC CTGCACGTCC AGGCGCAGGT GAACAACAGC 
AACAACAAGA AGGGTACCTT CACGGACGAC CTGCACAAGC TGGTGGACGA GTGGACGAGC 
AAGACGGTGG GGGCCGCGCA GCTGAAGCCC ACGCTCAACC AGCTGAAGCA GACCCAGAAG 
CTGCAAGACA TGGAGGCCCA GGCAGGCTGG GCTGCCCCTG GCGAGGCGCG GGCTATGACC 
GCACCTCGAG CAGGAGTGGG GATGCCACGT CTGCCCCCAG CGCCCGGCCC TCTGTCCACC 
ACGGTCATTC CCGGAGCCGC CCCGACCCTG TCCGTGCCCA CACCAGATGG CGCCCTCGGA 
ACCGCCCGGA GAAACCAGGT GTGGTTTGGC CTCCGAGTCC CCCCCACCGC CTGCTGTGGG 
CACAGCACTC AGCCGCGAGG GGGACAGCGG GTGGGCAGCA AGACTGCTTC CTTTGCGGCT 
TCAGACCCTG TTCGCTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870539.1
CDS	233..6913
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	XM_017015050.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015050.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGCAA GCTGCTAAAT 
CCCCTGGTGC GGCAGCTCAA GGTCGTGGCC TCCAGCACAG GTCACTTGGC TGACTCCAGC 
AGAGGCCCTC CCGCTAAGGA CCCTGCCCAA GCCAGTGTGG GGCTCACTGC AGACAGCACG 
GGCCTGAGCG GGAAGGCAGT GCAGACCCAG CAGCCCTGCT CCGTCCGGGC CTCCCTGTCT 
TCGGACATCT GCTCCGGCTT AGCCAGTGAT GGAGGCGGAG CGCGTGGCCA AGGGTCTGCC 
CTGAAGCGTC TCTGCCTAGG CAAAGAACAC AGCAGTAGGT CCTCCACCAG CAGCCTGGCC 
CCAGGCCCTG AGCCAGGCCC CCAGCCCGCC CTGCACGTCC AGGCGCAGGT GAACAACAGC 
AACAACAAGA AGGGTACCTT CACGGACGAC CTGCACAAGC TGGTGGACGA GTGGACGAGC 
AAGACGGTGG GGGCCGCGCA GCTGAAGCCC ACGCTCAACC AGCTGAAGCA GACCCAGAAG 
CTGCAAGACA TGGAGGCCCA GGCAGGCTGG GCTGCCCCTG GCGAGGCGCG GGCTATGACC 
GCACCTCGAG CAGGAGTGGG GATGCCACGT CTGCCCCCAG CGCCCGGCCC TCTGTCCACC 
ACGGTCATTC CCGGAGCCGC CCCGACCCTG TCCGTGCCCA CACCAGATGG CGCCCTCGGA 
ACCGCCCGGA GAAACCAGGT GTGGTTTGGC CTCCGAGTCC CCCCCACCGC CTGCTGTGGG 
CACAGCACTC AGCCGCGAGG GGGACAGCGG GTGGGCAGCA AGACTGCTTC CTTTGCGGCT 
TCAGACCCTG TTCGCTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_017015051.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870540.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X18, mRNA.

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CloneID	OHu98850
Clone ID Related Accession (Same CDS sequence)	XM_017015051.1
Accession Version	XM_017015051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGAGAAG 
TCAGAACTGG CCCCCACTCG AGGGGCCGTG ATGGAGCAGG GCACGTCCTC GTCAATGACA 
GCAGAGTCGT CTCCCAGGAG TATGCTAGGC TATGACAGAG ATGGAAGGCA GGTGGCCTCA 
GACTCCCATG TGGTCCCCAG CGTCCCCCAG GATGTACCTG CTTTTGTGAG ACCTGCACGT 
GTGGAGCCCA CAGACAGGGA TGGTGGAGAA GCTGGAGAAA GCTCGGCAGA GCCCCCGCCG 
AGTGACATGG GCACAGTGGG GGGCCAGGCT AGCCACCCCC AGACACTCGG CGCTCGAGCT 
TTGGGGTCCC CTCGGAAACG TCCAGAGCAG CAGGATGTCA GCTCACCAGC CAAGACTGTG 
GGCCGTTTCT CGGTGGTCAG CACTCAGGAC GAGTGGACCC TGGCCTCCCC CCACAGCCTG 
AGATACTCTG CCCCACCCGA CGTCTACCTG GACGAGGCCC CCTCCAGCCC CGACGTGAAG 
CTGGCAGTGC GGCGGGCGCA GACGGCCTCC TCCATCGAGG TCGGCGTGGG CGAGCCCGTG 
TCCAGCGACT CTGGGGACGA GGGCCCTCGG GCGAGACCCC CGGTGCAGAA GCAGGCGTCC 
CTGCCCGTGA GTGGCAGCGT GGCTGGCGAC TTCGTGAAGA AGGCCACCGC CTTCCTGCAG 
AGGCCTTCTC GGGCCGGCTC GCTGGGCCCC GAGACACCCA GCAGGGTGGG CATGAAGGTC 
CCCACGATCA GCGTGACCTC CTTCCATTCC CAGTCGTCCT ACATCAGCAG CGACAATGAT 
TCGGAGCTCG AGGATGCTGA CATAAAGAAG GAGCTGCAGA GTCTGCGGGA GAAGCACCTG 
AAGGAGATCT CGGAGCTGCA GAGCCAGCAG AAGCAGGAGA TCGAAGCTCT GTACCGCCGC 
CTGGGCAAGC CACTGCCCCC CAACGTGGGC TTCTTCCACA CGGCACCCCC CACTGGCCGC 
CGGAGAAAAA CCAGCAAGAG CAAGCTGAAG GCAGGCAAGC TGCTAAATCC CCTGGTGCGG 
CAGCTCAAGG TCGTGGCCTC CAGCACAGGT CACTTGGCTG ACTCCAGCAG AGGCCCTCCC 
GCTAAGGACC CTGCCCAAGC CAGTGTGGGG CTCACTGCAG ACAGCACGGG CCTGAGCGGG 
AAGGCAGTGC AGACCCAGCA GCCCTGCTCC GTCCGGGCCT CCCTGTCTTC GGACATCTGC 
TCCGGCTTAG CCAGTGATGG AGGCGGAGCG CGTGGCCAAG GCTGGACGGT TTACCACCCA 
ACGTCTGAGA GAGTGACCTA TAAGTCTAGT AGCAAACCTC GTGCTCGATT CCTCAGTGGA 
CCCGTATCTG TGTCCATCTG GTCTGCCCTG AAGCGTCTCT GCCTAGGCAA AGAACACAGC 
AGTAGGTCCT CCACCAGCAG CCTGGCCCCA GGCCCTGAGC CAGGCCCCCA GCCCGCCCTG 
CACGTCCAGG CGCAGGTGAA CAACAGCAAC AACAAGAAGG GTACCTTCAC GGACGACCTG 
CACAAGCTGG TGGACGAGTG GACGAGCAAG ACGGTGGGGG CCGCGCAGCT GAAGCCCACG 
CTCAACCAGC TGAAGCAGAC CCAGAAGCTG CAAGACATGG AGGCCCAGGC AGGCTGGGCT 
GCCCCTGGCG AGGCGCGGGC TATGACCGCA CCTCGAGCAG GAGTGGGGAT GCCACGTCTG 
CCCCCAGCGC CCGGCCCTCT GTCCACCACG GTCATTCCCG GAGCCGCCCC GACCCTGTCC 
GTGCCCACAC CAGATGGCGC CCTCGGAACC GCCCGGAGAA ACCAGGTGTG GTTTGGCCTC 
CGAGTCCCCC CCACCGCCTG CTGTGGGCAC AGCACTCAGC CGCGAGGGGG ACAGCGGGTG 
GGCAGCAAGA CTGCTTCCTT TGCGGCTTCA GACCCTGTTC GCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870540.1
CDS	233..6880
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	XM_017015051.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015051.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGAGAAG 
TCAGAACTGG CCCCCACTCG AGGGGCCGTG ATGGAGCAGG GCACGTCCTC GTCAATGACA 
GCAGAGTCGT CTCCCAGGAG TATGCTAGGC TATGACAGAG ATGGAAGGCA GGTGGCCTCA 
GACTCCCATG TGGTCCCCAG CGTCCCCCAG GATGTACCTG CTTTTGTGAG ACCTGCACGT 
GTGGAGCCCA CAGACAGGGA TGGTGGAGAA GCTGGAGAAA GCTCGGCAGA GCCCCCGCCG 
AGTGACATGG GCACAGTGGG GGGCCAGGCT AGCCACCCCC AGACACTCGG CGCTCGAGCT 
TTGGGGTCCC CTCGGAAACG TCCAGAGCAG CAGGATGTCA GCTCACCAGC CAAGACTGTG 
GGCCGTTTCT CGGTGGTCAG CACTCAGGAC GAGTGGACCC TGGCCTCCCC CCACAGCCTG 
AGATACTCTG CCCCACCCGA CGTCTACCTG GACGAGGCCC CCTCCAGCCC CGACGTGAAG 
CTGGCAGTGC GGCGGGCGCA GACGGCCTCC TCCATCGAGG TCGGCGTGGG CGAGCCCGTG 
TCCAGCGACT CTGGGGACGA GGGCCCTCGG GCGAGACCCC CGGTGCAGAA GCAGGCGTCC 
CTGCCCGTGA GTGGCAGCGT GGCTGGCGAC TTCGTGAAGA AGGCCACCGC CTTCCTGCAG 
AGGCCTTCTC GGGCCGGCTC GCTGGGCCCC GAGACACCCA GCAGGGTGGG CATGAAGGTC 
CCCACGATCA GCGTGACCTC CTTCCATTCC CAGTCGTCCT ACATCAGCAG CGACAATGAT 
TCGGAGCTCG AGGATGCTGA CATAAAGAAG GAGCTGCAGA GTCTGCGGGA GAAGCACCTG 
AAGGAGATCT CGGAGCTGCA GAGCCAGCAG AAGCAGGAGA TCGAAGCTCT GTACCGCCGC 
CTGGGCAAGC CACTGCCCCC CAACGTGGGC TTCTTCCACA CGGCACCCCC CACTGGCCGC 
CGGAGAAAAA CCAGCAAGAG CAAGCTGAAG GCAGGCAAGC TGCTAAATCC CCTGGTGCGG 
CAGCTCAAGG TCGTGGCCTC CAGCACAGGT CACTTGGCTG ACTCCAGCAG AGGCCCTCCC 
GCTAAGGACC CTGCCCAAGC CAGTGTGGGG CTCACTGCAG ACAGCACGGG CCTGAGCGGG 
AAGGCAGTGC AGACCCAGCA GCCCTGCTCC GTCCGGGCCT CCCTGTCTTC GGACATCTGC 
TCCGGCTTAG CCAGTGATGG AGGCGGAGCG CGTGGCCAAG GCTGGACGGT TTACCACCCA 
ACGTCTGAGA GAGTGACCTA TAAGTCTAGT AGCAAACCTC GTGCTCGATT CCTCAGTGGA 
CCCGTATCTG TGTCCATCTG GTCTGCCCTG AAGCGTCTCT GCCTAGGCAA AGAACACAGC 
AGTAGGTCCT CCACCAGCAG CCTGGCCCCA GGCCCTGAGC CAGGCCCCCA GCCCGCCCTG 
CACGTCCAGG CGCAGGTGAA CAACAGCAAC AACAAGAAGG GTACCTTCAC GGACGACCTG 
CACAAGCTGG TGGACGAGTG GACGAGCAAG ACGGTGGGGG CCGCGCAGCT GAAGCCCACG 
CTCAACCAGC TGAAGCAGAC CCAGAAGCTG CAAGACATGG AGGCCCAGGC AGGCTGGGCT 
GCCCCTGGCG AGGCGCGGGC TATGACCGCA CCTCGAGCAG GAGTGGGGAT GCCACGTCTG 
CCCCCAGCGC CCGGCCCTCT GTCCACCACG GTCATTCCCG GAGCCGCCCC GACCCTGTCC 
GTGCCCACAC CAGATGGCGC CCTCGGAACC GCCCGGAGAA ACCAGGTGTG GTTTGGCCTC 
CGAGTCCCCC CCACCGCCTG CTGTGGGCAC AGCACTCAGC CGCGAGGGGG ACAGCGGGTG 
GGCAGCAAGA CTGCTTCCTT TGCGGCTTCA GACCCTGTTC GCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_017015052.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870541.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X19, mRNA.

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CloneID	OHu98851
Clone ID Related Accession (Same CDS sequence)	XM_017015052.1
Accession Version	XM_017015052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG TCACTTGGCT 
GACTCCAGCA GAGGCCCTCC CGCTAAGGAC CCTGCCCAAG CCAGTGTGGG GCTCACTGCA 
GACAGCACGG GCCTGAGCGG GAAGGCAGTG CAGACCCAGC AGCCCTGCTC CGTCCGGGCC 
TCCCTGTCTT CGGACATCTG CTCCGGCTTA GCCAGTGATG GAGGCGGAGC GCGTGGCCAA 
GGGTCTGCCC TGAAGCGTCT CTGCCTAGGC AAAGAACACA GCAGTAGGTC CTCCACCAGC 
AGCCTGGCCC CAGGCCCTGA GCCAGGCCCC CAGCCCGCCC TGCACGTCCA GGCGCAGGTG 
AACAACAGCA ACAACAAGAA GGGTACCTTC ACGGACGACC TGCACAAGCT GGTGGACGAG 
TGGACGAGCA AGACGGTGGG GGCCGCGCAG CTGAAGCCCA CGCTCAACCA GCTGAAGCAG 
ACCCAGAAGC TGCAAGACAT GGAGGCCCAG GCAGGCTGGG CTGCCCCTGG CGAGGCGCGG 
GCTATGACCG CACCTCGAGC AGGAGTGGGG ATGCCACGTC TGCCCCCAGC GCCCGGCCCT 
CTGTCCACCA CGGTCATTCC CGGAGCCGCC CCGACCCTGT CCGTGCCCAC ACCAGATGGC 
GCCCTCGGAA CCGCCCGGAG AAACCAGGTG TGGTTTGGCC TCCGAGTCCC CCCCACCGCC 
TGCTGTGGGC ACAGCACTCA GCCGCGAGGG GGACAGCGGG TGGGCAGCAA GACTGCTTCC 
TTTGCGGCTT CAGACCCTGT TCGCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870541.1
CDS	233..6862
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	XM_017015052.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015052.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG TCACTTGGCT 
GACTCCAGCA GAGGCCCTCC CGCTAAGGAC CCTGCCCAAG CCAGTGTGGG GCTCACTGCA 
GACAGCACGG GCCTGAGCGG GAAGGCAGTG CAGACCCAGC AGCCCTGCTC CGTCCGGGCC 
TCCCTGTCTT CGGACATCTG CTCCGGCTTA GCCAGTGATG GAGGCGGAGC GCGTGGCCAA 
GGGTCTGCCC TGAAGCGTCT CTGCCTAGGC AAAGAACACA GCAGTAGGTC CTCCACCAGC 
AGCCTGGCCC CAGGCCCTGA GCCAGGCCCC CAGCCCGCCC TGCACGTCCA GGCGCAGGTG 
AACAACAGCA ACAACAAGAA GGGTACCTTC ACGGACGACC TGCACAAGCT GGTGGACGAG 
TGGACGAGCA AGACGGTGGG GGCCGCGCAG CTGAAGCCCA CGCTCAACCA GCTGAAGCAG 
ACCCAGAAGC TGCAAGACAT GGAGGCCCAG GCAGGCTGGG CTGCCCCTGG CGAGGCGCGG 
GCTATGACCG CACCTCGAGC AGGAGTGGGG ATGCCACGTC TGCCCCCAGC GCCCGGCCCT 
CTGTCCACCA CGGTCATTCC CGGAGCCGCC CCGACCCTGT CCGTGCCCAC ACCAGATGGC 
GCCCTCGGAA CCGCCCGGAG AAACCAGGTG TGGTTTGGCC TCCGAGTCCC CCCCACCGCC 
TGCTGTGGGC ACAGCACTCA GCCGCGAGGG GGACAGCGGG TGGGCAGCAA GACTGCTTCC 
TTTGCGGCTT CAGACCCTGT TCGCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_017015053.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870542.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X20, mRNA.

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CloneID	OHu98852
Clone ID Related Accession (Same CDS sequence)	XM_017015053.1
Accession Version	XM_017015053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGTCA CTTGGCTGAC 
TCCAGCAGAG GCCCTCCCGC TAAGGACCCT GCCCAAGCCA GTGTGGGGCT CACTGCAGAC 
AGCACGGGCC TGAGCGGGAA GGCAGTGCAG ACCCAGCAGC CCTGCTCCGT CCGGGCCTCC 
CTGTCTTCGG ACATCTGCTC CGGCTTAGCC AGTGATGGAG GCGGAGCGCG TGGCCAAGGG 
TCTGCCCTGA AGCGTCTCTG CCTAGGCAAA GAACACAGCA GTAGGTCCTC CACCAGCAGC 
CTGGCCCCAG GCCCTGAGCC AGGCCCCCAG CCCGCCCTGC ACGTCCAGGC GCAGGTGAAC 
AACAGCAACA ACAAGAAGGG TACCTTCACG GACGACCTGC ACAAGCTGGT GGACGAGTGG 
ACGAGCAAGA CGGTGGGGGC CGCGCAGCTG AAGCCCACGC TCAACCAGCT GAAGCAGACC 
CAGAAGCTGC AAGACATGGA GGCCCAGGCA GGCTGGGCTG CCCCTGGCGA GGCGCGGGCT 
ATGACCGCAC CTCGAGCAGG AGTGGGGATG CCACGTCTGC CCCCAGCGCC CGGCCCTCTG 
TCCACCACGG TCATTCCCGG AGCCGCCCCG ACCCTGTCCG TGCCCACACC AGATGGCGCC 
CTCGGAACCG CCCGGAGAAA CCAGGTGTGG TTTGGCCTCC GAGTCCCCCC CACCGCCTGC 
TGTGGGCACA GCACTCAGCC GCGAGGGGGA CAGCGGGTGG GCAGCAAGAC TGCTTCCTTT 
GCGGCTTCAG ACCCTGTTCG CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870542.1
CDS	233..6859
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	XM_017015053.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015053.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGTCA CTTGGCTGAC 
TCCAGCAGAG GCCCTCCCGC TAAGGACCCT GCCCAAGCCA GTGTGGGGCT CACTGCAGAC 
AGCACGGGCC TGAGCGGGAA GGCAGTGCAG ACCCAGCAGC CCTGCTCCGT CCGGGCCTCC 
CTGTCTTCGG ACATCTGCTC CGGCTTAGCC AGTGATGGAG GCGGAGCGCG TGGCCAAGGG 
TCTGCCCTGA AGCGTCTCTG CCTAGGCAAA GAACACAGCA GTAGGTCCTC CACCAGCAGC 
CTGGCCCCAG GCCCTGAGCC AGGCCCCCAG CCCGCCCTGC ACGTCCAGGC GCAGGTGAAC 
AACAGCAACA ACAAGAAGGG TACCTTCACG GACGACCTGC ACAAGCTGGT GGACGAGTGG 
ACGAGCAAGA CGGTGGGGGC CGCGCAGCTG AAGCCCACGC TCAACCAGCT GAAGCAGACC 
CAGAAGCTGC AAGACATGGA GGCCCAGGCA GGCTGGGCTG CCCCTGGCGA GGCGCGGGCT 
ATGACCGCAC CTCGAGCAGG AGTGGGGATG CCACGTCTGC CCCCAGCGCC CGGCCCTCTG 
TCCACCACGG TCATTCCCGG AGCCGCCCCG ACCCTGTCCG TGCCCACACC AGATGGCGCC 
CTCGGAACCG CCCGGAGAAA CCAGGTGTGG TTTGGCCTCC GAGTCCCCCC CACCGCCTGC 
TGTGGGCACA GCACTCAGCC GCGAGGGGGA CAGCGGGTGG GCAGCAAGAC TGCTTCCTTT 
GCGGCTTCAG ACCCTGTTCG CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_017015054.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870543.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X26, mRNA.

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CloneID	OHu98853
Clone ID Related Accession (Same CDS sequence)	XM_017015054.1
Accession Version	XM_017015054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGGTCTGC CCTGAAGCGT 
CTCTGCCTAG GCAAAGAACA CAGCAGTAGG TCCTCCACCA GCAGCCTGGC CCCAGGCCCT 
GAGCCAGGCC CCCAGCCCGC CCTGCACGTC CAGGCGCAGG TGAACAACAG CAACAACAAG 
AAGGGTACCT TCACGGACGA CCTGCACAAG CTGGTGGACG AGTGGACGAG CAAGACGGTG 
GGGGCCGCGC AGCTGAAGCC CACGCTCAAC CAGCTGAAGC AGACCCAGAA GCTGCAAGAC 
ATGGAGGCCC AGGCAGGCTG GGCTGCCCCT GGCGAGGCGC GGGCTATGAC CGCACCTCGA 
GCAGGAGTGG GGATGCCACG TCTGCCCCCA GCGCCCGGCC CTCTGTCCAC CACGGTCATT 
CCCGGAGCCG CCCCGACCCT GTCCGTGCCC ACACCAGATG GCGCCCTCGG AACCGCCCGG 
AGAAACCAGG TGTGGTTTGG CCTCCGAGTC CCCCCCACCG CCTGCTGTGG GCACAGCACT 
CAGCCGCGAG GGGGACAGCG GGTGGGCAGC AAGACTGCTT CCTTTGCGGC TTCAGACCCT 
GTTCGCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870543.1
CDS	233..6724
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	XM_017015054.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015054.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGCAGA GTCGTCTCCC AGGAGTATGC TAGGCTATGA CAGAGATGGA 
AGGCAGGTGG CCTCAGACTC CCATGTGGTC CCCAGCGTCC CCCAGGATGT ACCTGCTTTT 
GTGAGACCTG CACGTGTGGA GCCCACAGAC AGGGATGGTG GAGAAGCTGG AGAAAGCTCG 
GCAGAGCCCC CGCCGAGTGA CATGGGCACA GTGGGGGGCC AGGCTAGCCA CCCCCAGACA 
CTCGGCGCTC GAGCTTTGGG GTCCCCTCGG AAACGTCCAG AGCAGCAGGA TGTCAGCTCA 
CCAGCCAAGA CTGTGGGCCG TTTCTCGGTG GTCAGCACTC AGGACGAGTG GACCCTGGCC 
TCCCCCCACA GCCTGAGATA CTCTGCCCCA CCCGACGTCT ACCTGGACGA GGCCCCCTCC 
AGCCCCGACG TGAAGCTGGC AGTGCGGCGG GCGCAGACGG CCTCCTCCAT CGAGGTCGGC 
GTGGGCGAGC CCGTGTCCAG CGACTCTGGG GACGAGGGCC CTCGGGCGAG ACCCCCGGTG 
CAGAAGCAGG CGTCCCTGCC CGTGAGTGGC AGCGTGGCTG GCGACTTCGT GAAGAAGGCC 
ACCGCCTTCC TGCAGAGGCC TTCTCGGGCC GGCTCGCTGG GCCCCGAGAC ACCCAGCAGG 
GTGGGCATGA AGGTCCCCAC GATCAGCGTG ACCTCCTTCC ATTCCCAGTC GTCCTACATC 
AGCAGCGACA ATGATTCGGA GCTCGAGGAT GCTGACATAA AGAAGGAGCT GCAGAGTCTG 
CGGGAGAAGC ACCTGAAGGA GATCTCGGAG CTGCAGAGCC AGCAGAAGCA GGAGATCGAA 
GCTCTGTACC GCCGCCTGGG CAAGCCACTG CCCCCCAACG TGGGCTTCTT CCACACGGCA 
CCCCCCACTG GCCGCCGGAG AAAAACCAGC AAGAGCAAGC TGAAGGCAGG CAAGCTGCTA 
AATCCCCTGG TGCGGCAGCT CAAGGTCGTG GCCTCCAGCA CAGGGTCTGC CCTGAAGCGT 
CTCTGCCTAG GCAAAGAACA CAGCAGTAGG TCCTCCACCA GCAGCCTGGC CCCAGGCCCT 
GAGCCAGGCC CCCAGCCCGC CCTGCACGTC CAGGCGCAGG TGAACAACAG CAACAACAAG 
AAGGGTACCT TCACGGACGA CCTGCACAAG CTGGTGGACG AGTGGACGAG CAAGACGGTG 
GGGGCCGCGC AGCTGAAGCC CACGCTCAAC CAGCTGAAGC AGACCCAGAA GCTGCAAGAC 
ATGGAGGCCC AGGCAGGCTG GGCTGCCCCT GGCGAGGCGC GGGCTATGAC CGCACCTCGA 
GCAGGAGTGG GGATGCCACG TCTGCCCCCA GCGCCCGGCC CTCTGTCCAC CACGGTCATT 
CCCGGAGCCG CCCCGACCCT GTCCGTGCCC ACACCAGATG GCGCCCTCGG AACCGCCCGG 
AGAAACCAGG TGTGGTTTGG CCTCCGAGTC CCCCCCACCG CCTGCTGTGG GCACAGCACT 
CAGCCGCGAG GGGGACAGCG GGTGGGCAGC AAGACTGCTT CCTTTGCGGC TTCAGACCCT 
GTTCGCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK2 ( XM_017015055.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870544.1 Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X27, mRNA.

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CloneID	OHu98854
Clone ID Related Accession (Same CDS sequence)	XM_017015055.1
Accession Version	XM_017015055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK2 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGCAA GCTGCTAAAT 
CCCCTGGTGC GGCAGCTCAA GGTCGTGGCC TCCAGCACAG GGTCTGCCCT GAAGCGTCTC 
TGCCTAGGCA AAGAACACAG CAGTAGGTCC TCCACCAGCA GCCTGGCCCC AGGCCCTGAG 
CCAGGCCCCC AGCCCGCCCT GCACGTCCAG GCGCAGGTGA ACAACAGCAA CAACAAGAAG 
GGTACCTTCA CGGACGACCT GCACAAGCTG GTGGACGAGT GGACGAGCAA GACGGTGGGG 
GCCGCGCAGC TGAAGCCCAC GCTCAACCAG CTGAAGCAGA CCCAGAAGCT GCAAGACATG 
GAGGCCCAGG CAGGCTGGGC TGCCCCTGGC GAGGCGCGGG CTATGACCGC ACCTCGAGCA 
GGAGTGGGGA TGCCACGTCT GCCCCCAGCG CCCGGCCCTC TGTCCACCAC GGTCATTCCC 
GGAGCCGCCC CGACCCTGTC CGTGCCCACA CCAGATGGCG CCCTCGGAAC CGCCCGGAGA 
AACCAGGTGT GGTTTGGCCT CCGAGTCCCC CCCACCGCCT GCTGTGGGCA CAGCACTCAG 
CCGCGAGGGG GACAGCGGGT GGGCAGCAAG ACTGCTTCCT TTGCGGCTTC AGACCCTGTT 
CGCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870544.1
CDS	233..6721
Translation	MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS

Target ORF information:

RefSeq Version	XM_017015055.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 2 (WNK2), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015055.1

ORF Insert Sequence:

ATGGACGGCG ATGGCGGCCG CCGAGACGTC CCCGGCACGC TGATGGAGCC CGGGCGCGGC 
GCGGGGCCCG CGGGCATGGC GGAGCCTCGG GCGAAGGCGG CGCGGCCGGG GCCCCAGCGC 
TTTCTGCGGC GCAGCGTGGT AGAGTCGGAC CAGGAGGAGC CGCCGGGCTT GGAGGCAGCC 
GAGGCGCCGG GCCCGCAGCC CCCGCAGCCC CTGCAGCGCC GGGTGCTTCT GCTCTGCAAG 
ACGCGCCGCC TCATCGCGGA GCGCGCCCGC GGACGCCCCG CCGCCCCCGC GCCCGCAGCG 
CTGGTAGCGC AGCCGGGAGC CCCCGGAGCC CCCGCGGACG CCGGCCCCGA GCCCGTGGGC 
ACGCAGGAGC CCGGCCCGGA CCCCATCGCA GCCGCTGTCG AAACCGCGCC TGCCCCCGAC 
GGCGGCCCCA GGGAGGAGGC GGCGGCGACC GTGAGGAAGG AGGATGAGGG GGCGGCCGAG 
GCGAAGCCTG AGCCCGGGCG CACTCGCCGG GACGAGCCCG AAGAGGAGGA GGACGACGAG 
GACGACCTCA AGGCCGTGGC CACCTCTCTG GACGGCCGCT TCCTCAAGTT CGACATCGAG 
CTGGGCCGCG GTTCCTTCAA GACGGTCTAC AAGGGGCTGG ACACGGAGAC CTGGGTGGAG 
GTGGCCTGGT GTGAGCTGCA GGACCGGAAG CTCACCAAGC TGGAGCGGCA GCGGTTCAAG 
GAAGAGGCTG AGATGCTGAA AGGCCTGCAG CACCCCAACA TCGTGCGCTT CTACGACTTC 
TGGGAGTCCA GCGCCAAGGG CAAGCGGTGC ATTGTGCTGG TGACGGAGCT GATGACCTCA 
GGGACGCTGA AGACATACCT GAAGCGGTTC AAGGTGATGA AGCCCAAGGT TCTCCGCAGC 
TGGTGCCGGC AGATCCTGAA GGGCCTGCTG TTCCTGCACA CAAGGACGCC ACCCATCATC 
CACCGAGACC TGAAATGTGA CAATATTTTC ATCACCGGAC CAACTGGGTC TGTGAAGATT 
GGCGACTTGG GCCTGGCCAC TCTGAAAAGA GCGTCATTTG CCAAAAGTGT GATAGGTACT 
CCCGAGTTCA TGGCGCCCGA GATGTACGAG GAGCACTACG ATGAGTCCGT GGACGTCTAT 
GCCTTTGGGA TGTGCATGCT GGAGATGGCC ACCTCGGAGT ACCCCTACTC GGAGTGCCAG 
AATGCGGCCC AGATCTACCG CAAGGTCACC TGTGGTATCA AGCCGGCCAG CTTTGAGAAA 
GTGCACGATC CTGAAATCAA GGAGATTATT GGGGAGTGTA TCTGCAAAAA CAAGGAGGAA 
AGGTACGAGA TCAAAGACCT GCTGAGCCAC GCCTTCTTCG CAGAGGACAC AGGCGTGAGG 
GTGGAGCTCG CGGAGGAGGA CCACGGCAGG AAGTCCACCA TCGCCCTGAG GCTCTGGGTG 
GAAGACCCCA AGAAACTGAA GGGAAAGCCC AAGGACAATG GAGCCATAGA GTTCACCTTC 
GACCTGGAGA AGGAGACGCC GGATGAGGTG GCCCAAGAGA TGATTGAGTC TGGATTCTTC 
CACGAGAGTG ACGTCAAGAT CGTGGCCAAG TCCATCCGTG ACCGCGTGGC CTTGATCCAG 
TGGCGGCGGG AGAGGATCTG GCCCGCGCTG CAGCCCAAGG AGCAGCAGGA TGTGGGCAGC 
CCGGACAAGG CCAGGGGTCC GCCGGTGCCC CTGCAGGTCC AGGTGACCTA CCATGCACAG 
GCTGGGCAGC CCGGGCCACC AGAGCCCGAG GAGCCGGAGG CCGACCAGCA CCTCCTGCCA 
CCTACGTTGC CGACCAGCGC CACCTCCCTG GCCTCGGACA GCACCTTCGA CAGCGGCCAG 
GGCTCTACCG TGTACTCAGA CTCGCAGAGC AGCCAGCAGA GCGTGATGCT TGGCTCCCTT 
GCCGACGCAG CGCCGTCCCC GGCCCAGTGT GTGTGCAGCC CCCCTGTGAG CGAGGGGCCC 
GTCCTGCCGC AGAGCCTGCC CTCGCTGGGG GCCTACCAGC AGCCCACGGC TGCACCTGGC 
TTGCCGGTGG GCTCTGTCCC GGCCCCCGCC TGCCCTCCGT CCCTCCAGCA GCACTTCCCG 
GATCCGGCCA TGAGCTTCGC CCCCGTGCTG CCGCCGCCCA GCACCCCCAT GCCCACGGGC 
CCAGGCCAGC CAGCACCCCC CGGCCAGCAG CCTCCTCCGC TGGCCCAGCC GACACCCCTG 
CCGCAGGTCC TGGCCCCACA GCCCGTGGTC CCCCTCCAGC CGGTTCCCCC CCACCTGCCA 
CCGTACCTGG CTCCAGCCTC CCAGGTGGGG GCCCCCGCTC AGCTGAAGCC CCTCCAGATG 
CCACAGGCGC CCCTGCAGCC GCTTGCTCAA GTCCCTCCGC AGATGCCCCC GATTCCTGTT 
GTGCCCCCCA TCACGCCCCT GGCGGGAATC GACGGCCTCC CTCCGGCCCT CCCAGACCTG 
CCGACCGCGA CTGTGCCTCC CGTGCCACCA CCTCAGTATT TCTCTCCAGC CGTGATCTTG 
CCGAGCCTCG CTGCCCCACT CCCCCCTGCG TCCCCAGCCT TGCCTCTGCA GGCTGTGAAG 
CTGCCCCACC CCCCTGGGGC GCCCCTGGCC ATGCCCTGCC GGACCATTGT GCCAAATGCA 
CCGGCCACTA TCCCCCTGCT GGCCGTAGCC CCACCGGGCG TGGCTGCCCT GTCCATTCAT 
TCTGCCGTGG CCCAGCTCCC AGGCCAACCT GTGTACCCAG CGGCCTTCCC ACAGATGGCG 
CCTACTGACG TCCCTCCTTC CCCCCATCAC ACGGTGCAGA ATATGAGGGC CACCCCTCCA 
CAGCCGGCAC TGCCTCCACA ACCCACACTG CCCCCACAAC CCGTGCTGCC CCCGCAACCC 
ACGCTGCCCC CTCAACCTGT GTTGCCCCCG CAACCCACAC GGCCCCCTCA ACCTGTGCTG 
CCCCCGCAAC CCATGCTGCC CCCACAACCT GTGCTGCCCC CGCAGCCGGC ACTGCCTGTG 
CGCCCTGAGC CCCTCCAGCC CCACCTTCCT GAACAAGCTG CTCCAGCTGC TACACCAGGG 
AGCCAGATTC TGCTTGGCCA CCCAGCTCCC TATGCTGTGG ACGTCGCCGC TCAGGTCCCC 
ACCGTGCCTG TGCCACCGGC TGCGGTCCTC TCGCCGCCTC TGCCGGAAGT GCTGCTGCCT 
GCCGCCCCTG AGCTCCTGCC TCAGTTCCCC AGCTCCCTGG CCACGGTGTC TGCCTCTGTG 
CAGAGTGTGC CCACCCAGAC TGCCACACTT CTGCCACCAG CAAACCCACC GCTGCCTGGC 
GGGCCCGGGA TCGCCAGCCC TTGCCCAACT GTCCAGCTGA CGGTGGAACC AGTCCAAGAG 
GAGCAGGCCT CACAGGACAA GCCGCCCGGC CTCCCGCAGA GCTGTGAGAG CTATGGAGGT 
TCTGATGTCA CTTCTGGAAA AGAGCTGAGT GACAGCTGTG AAGGCGCCTT TGGAGGGGGC 
AGGCTGGAGG GCAGGGCAGC CCGAAAACAC CACCGCAGGT CCACGCGTGC GCGCTCCCGG 
CAGGAGAGGG CCAGCCGGCC CCGGCTTACC ATCTTGAACG TGTGCAACAC TGGGGACAAG 
ATGGTGGAGT GCCAGCTGGA GACGCACAAC CACAAGATGG TGACCTTCAA GTTCGACTTG 
GACGGGGACG CACCCGATGA AATTGCCACG TATATGGTGG AGCATGACTT TATCCTGCAG 
GCCGAGCGGG AAACGTTCAT CGAGCAGATG AAGGATGTCA TGGACAAGGC AGAGGACATG 
CTCAGCGAGG ACACAGACGC CGACCGTGGC TCCGACCCAG GGACCAGCCC GCCACACCTC 
AGCACCTGCG GCCTGGGCAC CGGGGAGGAG AGCCGACAAT CCCAAGCCAA CGCCCCCGTG 
TATCAGCAGA ACGTCCTGCA CACCGGGAAG AGGTGGTTCA TCATCTGTCC GGTGGCTGAG 
CACCCCGCCC CCGAGGCCCC TGAATCTTCG CCCCCACTTC CTCTAAGCTC CCTGCCGCCA 
GAAGCCAGCC AAGATTCAGC GCCCTATAAA GACCAGCTGT CCTCGAAGGA ACAACCCAGC 
TTTCTAGCCA GTCAGCAGCT CCTGAGCCAG GCGGGCCCCA GCAACCCTCC TGGGGCACCC 
CCAGCCCCTT TGGCCCCCTC CTCCCCTCCT GTGACTGCTC TGCCCCAAGA TGGAGCAGCT 
CCAGCCACCA GCACCATGCC AGAGCCAGCG TCAGGAACTG CCAGCCAGGC AGGGGGTCCA 
GGGACACCTC AGGGGCTGAC CAGTGAGCTC GAGACGTCTC AGCCACTAGC GGAGACTCAC 
GAGGCCCCGC TTGCTGTGCA GCCCCTCGTG GTGGGCCTAG CACCTTGCAC TCCAGCTCCA 
GAGGCTGCCT CAACCAGGGA CGCCAGTGCC CCAAGGGAGC CCCTGCCACC TCCTGCACCT 
GAGCCCAGCC CCCACAGCGG GACCCCACAG CCCGCCTTGG GTCAACCTGC TCCCCTGCTT 
CCTGCCGCAG TGGGGGCCGT CAGCCTGGCC ACCTCCCAGC TCCCAAGCCC ACCCCTGGGG 
CCCACCGTCC CCCCACAGCC ACCCTCGGCC CTGGAGTCGG ATGGGGAAGG GCCGCCCCCC 
AGGGTGGGCT TTGTGGACAG CACCATCAAG AGCCTGGACG AGAAGCTGCG GACTCTGCTC 
TACCAGGAGC ACGTGCCCAC CTCCTCAGCC TCAGCTGGGA CCCCTGTGGA GGTGGGCGAC 
AGAGACTTCA CCCTGGAGCC CCTGAGAGGG GACCAGCCCC GCTCAGAGGT CTGCGGGGGG 
GACCTGGCCC TGCCCCCAGT GCCTAAGGAG GCGGTCTCAG GGCGTGTCCA GCTGCCCCAG 
CCCTTGGTGG AGAAGTCAGA ACTGGCCCCC ACTCGAGGGG CCGTGATGGA GCAGGGCACG 
TCCTCGTCAA TGACAGAGTC GTCTCCCAGG AGTATGCTAG GCTATGACAG AGATGGAAGG 
CAGGTGGCCT CAGACTCCCA TGTGGTCCCC AGCGTCCCCC AGGATGTACC TGCTTTTGTG 
AGACCTGCAC GTGTGGAGCC CACAGACAGG GATGGTGGAG AAGCTGGAGA AAGCTCGGCA 
GAGCCCCCGC CGAGTGACAT GGGCACAGTG GGGGGCCAGG CTAGCCACCC CCAGACACTC 
GGCGCTCGAG CTTTGGGGTC CCCTCGGAAA CGTCCAGAGC AGCAGGATGT CAGCTCACCA 
GCCAAGACTG TGGGCCGTTT CTCGGTGGTC AGCACTCAGG ACGAGTGGAC CCTGGCCTCC 
CCCCACAGCC TGAGATACTC TGCCCCACCC GACGTCTACC TGGACGAGGC CCCCTCCAGC 
CCCGACGTGA AGCTGGCAGT GCGGCGGGCG CAGACGGCCT CCTCCATCGA GGTCGGCGTG 
GGCGAGCCCG TGTCCAGCGA CTCTGGGGAC GAGGGCCCTC GGGCGAGACC CCCGGTGCAG 
AAGCAGGCGT CCCTGCCCGT GAGTGGCAGC GTGGCTGGCG ACTTCGTGAA GAAGGCCACC 
GCCTTCCTGC AGAGGCCTTC TCGGGCCGGC TCGCTGGGCC CCGAGACACC CAGCAGGGTG 
GGCATGAAGG TCCCCACGAT CAGCGTGACC TCCTTCCATT CCCAGTCGTC CTACATCAGC 
AGCGACAATG ATTCGGAGCT CGAGGATGCT GACATAAAGA AGGAGCTGCA GAGTCTGCGG 
GAGAAGCACC TGAAGGAGAT CTCGGAGCTG CAGAGCCAGC AGAAGCAGGA GATCGAAGCT 
CTGTACCGCC GCCTGGGCAA GCCACTGCCC CCCAACGTGG GCTTCTTCCA CACGGCACCC 
CCCACTGGCC GCCGGAGAAA AACCAGCAAG AGCAAGCTGA AGGCAGGCAA GCTGCTAAAT 
CCCCTGGTGC GGCAGCTCAA GGTCGTGGCC TCCAGCACAG GGTCTGCCCT GAAGCGTCTC 
TGCCTAGGCA AAGAACACAG CAGTAGGTCC TCCACCAGCA GCCTGGCCCC AGGCCCTGAG 
CCAGGCCCCC AGCCCGCCCT GCACGTCCAG GCGCAGGTGA ACAACAGCAA CAACAAGAAG 
GGTACCTTCA CGGACGACCT GCACAAGCTG GTGGACGAGT GGACGAGCAA GACGGTGGGG 
GCCGCGCAGC TGAAGCCCAC GCTCAACCAG CTGAAGCAGA CCCAGAAGCT GCAAGACATG 
GAGGCCCAGG CAGGCTGGGC TGCCCCTGGC GAGGCGCGGG CTATGACCGC ACCTCGAGCA 
GGAGTGGGGA TGCCACGTCT GCCCCCAGCG CCCGGCCCTC TGTCCACCAC GGTCATTCCC 
GGAGCCGCCC CGACCCTGTC CGTGCCCACA CCAGATGGCG CCCTCGGAAC CGCCCGGAGA 
AACCAGGTGT GGTTTGGCCT CCGAGTCCCC CCCACCGCCT GCTGTGGGCA CAGCACTCAG 
CCGCGAGGGG GACAGCGGGT GGGCAGCAAG ACTGCTTCCT TTGCGGCTTC AGACCCTGTT 
CGCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Silencing of the tumor suppressor gene WNK2 is associated with upregulation of MMP2 and JNK in gliomas.
Oncotarget6(3)1422-34(2015 Jan)
Costa AM,Pinto F,Martinho O,Oliveira MJ,Jordan P,Reis RM

Early epigenetic downregulation of WNK2 kinase during pancreatic ductal adenocarcinoma development.
Oncogene33(26)3401-10(2014 Jun)
Dutruel C,Bergmann F,Rooman I,Zucknick M,Weichenhan D,Geiselhart L,Kaffenberger T,Rachakonda PS,Bauer A,Giese N,Hong C,Xie H,Costello JF,Hoheisel J,Kumar R,Rehli M,Schirmacher P,Werner J,Plass C,Popanda O,Schmezer P

Wnk kinases are positive regulators of canonical Wnt/?-catenin signalling.
EMBO reports14(8)718-25(2013 Aug)
Serysheva E,Berhane H,Grumolato L,Demir K,Balmer S,Bodak M,Boutros M,Aaronson S,Mlodzik M,Jenny A

Loss of WNK2 expression by promoter gene methylation occurs in adult gliomas and triggers Rac1-mediated tumour cell invasiveness.
Human molecular genetics22(1)84-95(2013 Jan)
Moniz S,Martinho O,Pinto F,Sousa B,Loureiro C,Oliveira MJ,Moita LF,Honavar M,Pinheiro C,Pires M,Lopes JM,Jones C,Costello JF,Paredes J,Reis RM,Jordan P

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WNK2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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