SMTN cDNA ORF clone, Homo sapiens(human)

The following SMTN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMTN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu14968 NM_001207017.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu15148 NM_006932.5
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu60562 XM_011530341.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu14920 NM_134270.3
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $657.30
$939.00
OHu15270 NM_001207018.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu18905 NM_134269.3
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu53659 XM_006724298.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu53663 XM_006724302.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu60555 XM_017028914.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu60556 XM_017028915.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu60557 XM_011530336.2
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu60558 XM_011530337.2
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu60559 XM_011530338.2
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu60560 XM_011530339.2
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu60561 XM_011530340.2
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu93564 XM_017028916.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu93565 XM_017028917.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu93566 XM_017028918.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu93567 XM_017028919.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu14968 XM_017028913.1
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu60554 XM_011530333.3
Latest version!
Homo sapiens smoothelin (SMTN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu60554 XM_011530333.2 Homo sapiens smoothelin (SMTN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu18905 NM_134269.2 Homo sapiens smoothelin (SMTN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu15148 NM_006932.4 Homo sapiens smoothelin (SMTN), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu14920 NM_134270.2 Homo sapiens smoothelin (SMTN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $657.30
$939.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu14968
    Clone ID Related Accession (Same CDS sequence) XM_017028913.1 , NM_001207017.1
    Accession Version NM_001207017.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Homo sapiens(human)
    Product smoothelin isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK304084.1, Z49989.2 and BC034237.2. Transcript Variant: This variant (4) encodes the longest isoform (d). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA GCCCTGGCGG ACCCCGCGCA GCCGTGCAGC GATCCACCAG CTTCGGGGTC
    CCCAACGCCA ACAGCATCAA GCAGATGCTG CTGGACTGGT GTCGAGCCAA GACTCGCGGC
    TACGAGCACG TCGACATCCA GAACTTCTCC TCCAGCTGGA GTGATGGGAT GGCCTTCTGT
    GCCCTGGTGC ACAACTTCTT CCCTGAGGCC TTCGACTATG GGCAGCTTAG CCCTCAGAAC
    CGACGCCAGA ACTTCGAGGT GGCCTTCTCA TCTGCGGAGA TGCTGGTGGA CTGTGTGCCC
    CTGGTGGAGG TGGACGACAT GATGATCATG GGCAAGAAGC CTGACCCCAA GTGTGTCTTC
    ACCTATGTGC AGTCGCTCTA CAACCACCTG CGACGCCACG AACTGCGCCT GCGCGGCAAG
    AATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193946.1
    CDS62..3070
    Translation

    Target ORF information:

    RefSeq Version NM_001207017.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001207017.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA GCCCTGGCGG ACCCCGCGCA GCCGTGCAGC GATCCACCAG CTTCGGGGTC
    CCCAACGCCA ACAGCATCAA GCAGATGCTG CTGGACTGGT GTCGAGCCAA GACTCGCGGC
    TACGAGCACG TCGACATCCA GAACTTCTCC TCCAGCTGGA GTGATGGGAT GGCCTTCTGT
    GCCCTGGTGC ACAACTTCTT CCCTGAGGCC TTCGACTATG GGCAGCTTAG CCCTCAGAAC
    CGACGCCAGA ACTTCGAGGT GGCCTTCTCA TCTGCGGAGA TGCTGGTGGA CTGTGTGCCC
    CTGGTGGAGG TGGACGACAT GATGATCATG GGCAAGAAGC CTGACCCCAA GTGTGTCTTC
    ACCTATGTGC AGTCGCTCTA CAACCACCTG CGACGCCACG AACTGCGCCT GCGCGGCAAG
    AATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15148
    Clone ID Related Accession (Same CDS sequence) NM_006932.5 , NM_006932.4
    Accession Version NM_006932.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product smoothelin isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC342566.1, BC034237.2, Z49989.2 and AJ010306.2. On Jun 2, 2019 this sequence version replaced NM_006932.4. Transcript Variant: This variant (3) has an alternate 5' exon, which results in a downstream AUG start codon, and lacks an in-frame exon in the middle region, compared to variant 4. The resulting isoform (c, also referred to as L1, B and c1) is shorter at the N-terminus and lacks an internal segment, compared to isoform d. This isoform is highly expressed in all vascularized organs and represents a vascular smooth muscle-specific form. This transcript also includes an alternate downstream translation initiation site, which results in the smoothelin-A product, a visceral smooth muscle-specific form. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ010306.2, SRR3476690.877847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGATGCTG GTGGACTGTG TGCCCCTGGT GGAGGTGGAC
    GACATGATGA TCATGGGCAA GAAGCCTGAC CCCAAGTGTG TCTTCACCTA TGTGCAGTCG
    CTCTACAACC ACCTGCGACG CCACGAACTG CGCCTGCGCG GCAAGAATGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_008863.3
    CDS220..2973
    Translation

    Target ORF information:

    RefSeq Version NM_006932.5
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006932.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGATGCTG GTGGACTGTG TGCCCCTGGT GGAGGTGGAC
    GACATGATGA TCATGGGCAA GAAGCCTGAC CCCAAGTGTG TCTTCACCTA TGTGCAGTCG
    CTCTACAACC ACCTGCGACG CCACGAACTG CGCCTGCGCG GCAAGAATGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15148
    Clone ID Related Accession (Same CDS sequence) NM_006932.5 , NM_006932.4
    Accession Version NM_006932.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product smoothelin isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC342566.1, BC034237.2, Z49989.2 and AJ010306.2. On May 18, 2011 this sequence version replaced NM_006932.3. Transcript Variant: This variant (3) has an alternate 5' exon, which results in a downstream AUG start codon, and lacks an in-frame exon in the middle region, compared to variant 4. The resulting isoform (c, also referred to as L1, B and c1) is shorter at the N-terminus and lacks an internal segment, compared to isoform d. This isoform is highly expressed in all vascularized organs and represents a vascular smooth muscle-specific form. This transcript also includes an alternate downstream translation initiation site, which results in the smoothelin-A product, a visceral smooth muscle-specific form. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ010306.2, Y13492.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGATGCTG GTGGACTGTG TGCCCCTGGT GGAGGTGGAC
    GACATGATGA TCATGGGCAA GAAGCCTGAC CCCAAGTGTG TCTTCACCTA TGTGCAGTCG
    CTCTACAACC ACCTGCGACG CCACGAACTG CGCCTGCGCG GCAAGAATGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_008863.3
    CDS242..2995
    Translation

    Target ORF information:

    RefSeq Version NM_006932.4
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006932.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGATGCTG GTGGACTGTG TGCCCCTGGT GGAGGTGGAC
    GACATGATGA TCATGGGCAA GAAGCCTGAC CCCAAGTGTG TCTTCACCTA TGTGCAGTCG
    CTCTACAACC ACCTGCGACG CCACGAACTG CGCCTGCGCG GCAAGAATGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60562
    Clone ID Related Accession (Same CDS sequence) XM_011530341.1
    Accession Version XM_011530341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCTGCTA GGCTCCAGGA TGGCACACCC CAGGCTGCCC TAAGTCCCCT GACCCCCGCA 
    AGGCTCCTGG GCCCCTCCCT CACCAGCACC ACCCCTGCCT CCTCCTCCAG CGGCTCCTCC
    TCTCGGGGCC CCAGTGATAC CTCCTCCCGG TTCAGCAAGG AGCAACGAGG AGTAGCCCAG
    CCCCTGGCCC AGCTTCGAAG CTGCCCCCAG GAGGAGGGCC CCAGGGGGCG GGGCTTGGCT
    GCTAGGCCCC TTGAAAACAG AGCAGGGGGG CCTGTGGCAC GTTCAGAGGA GCCTGGTGCC
    CCGCTGCCCG TGGCCGTCGG CACTGCCGAG CCAGGGGGCA GTATGAAGAC CACATTCACC
    ATCGAGATCA AGGACGGCCG TGGCCAGGCC TCCACAGGCC GGGTGCTGCT GCCCACAGGC
    AACCAGAGGG CAGAACTGAC ACTGGGGCTG CGGGCGCCCC CGACCCTACT CAGCACCAGT
    AGTGGGGGCA AGAGCACCAT CACCCGTGTC AACAGCCCTG GGACCCTGGC TCGGCTGGGC
    AGTGTCACTC ATGTCACCAG CTTCAGCCAT GCCCCCCCCA GTAGCCGAGG AGGCTGCAGC
    ATCAAGGCTG CCGAGGATGC CGGGACCCCC GTGGCCCACC CACCTGCCTT CAGCACCCGC
    CGCCGCTCCT CCACCGGCAC CACCCGCAGC ACTAGTCTCA TGGAAGCAGA GCCAGCAGAG
    CCTCTCGCTG CAGCAGTGGA AGCGGCCAAT GGGGCTGAGC AGACCCGAGT GAACAAAGCA
    CCAGAAGGGC GGAGCCCTCT GAGCGCTGAG GAGCTGATGA CTATTGAGGA TGAAGGAGTC
    TTGGACAAGA TGCTGGATCA GAGCACGGAC TTTGAAGAGC GGAAGCTCAT CCGGGCTGCA
    CTTCGTGAGC TCCGACAAAG GAAGAGAGAC CAGCGGGACA AGGAGCGGGA ACGGCGGCTG
    CAGGAGGCAC GGGGCCGGCC AGGGGAGGGG CGCGGCAACA CAGCCACTGA GACCACCACG
    AGGCACAGCC AGCGGGCAGC TGATGGCTCT GCTGTCAGCA CTGTTACCAA GACTGAGCGG
    CTCGTCCACT CCAATGATGG CACACGGACG GCCCGCACCA CCACAGTGGA GTCGAGTTTC
    GTGAGGCGCT CGGAGAATGG CAGTGGCAGC ACCATGATGC AAACCAAGAC CTTCTCCTCT
    TCCTCCTCAT CCAAGAAGAT GGGCAGCATC TTCGACCGCG AGGACCAGGC CAGCCCACGG
    GCCGGCAGCC TGGCGGCGCT CGAGAAACGG CAGGCCGAGA AGAAGAAAGA GCTGATGAAG
    GCGCAGAGTC TGCCCAAGAC CTCAGCCTCC CAGGCGCGCA AGGCCATGAT TGAGAAGCTG
    GAGAAGGAGG GCGCGGCCGG CAGCCCTGGC GGACCCCGCG CAGCCGTGCA GCGATCCACC
    AGCTTCGGGG TCCCCAACGC CAACAGCATC AAGCAGATGC TGCTGGACTG GTGTCGAGCC
    AAGACTCGCG GCTACGAGCA CGTCGACATC CAGAACTTCT CCTCCAGCTG GAGTGATGGG
    ATGGCCTTCT GTGCCCTGGT GCACAACTTC TTCCCTGAGG CCTTCGACTA TGGGCAGCTT
    AGCCCTCAGA ACCGACGCCA GAACTTCGAG GTGGCCTTCT CATCTGCGGA GATGCTGGTG
    GACTGTGTGC CCCTGGTGGA GGTGGACGAC ATGATGATCA TGGGCAAGAA GCCTGACCCC
    AAGTGTGTCT TCACCTATGT GCAGTCGCTC TACAACCACC TGCGACGCCA CGAACTGCGC
    CTGCGCGGCA AGAATGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528643.1
    CDS183..2003
    Translation

    Target ORF information:

    RefSeq Version XM_011530341.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCTGCTA GGCTCCAGGA TGGCACACCC CAGGCTGCCC TAAGTCCCCT GACCCCCGCA 
    AGGCTCCTGG GCCCCTCCCT CACCAGCACC ACCCCTGCCT CCTCCTCCAG CGGCTCCTCC
    TCTCGGGGCC CCAGTGATAC CTCCTCCCGG TTCAGCAAGG AGCAACGAGG AGTAGCCCAG
    CCCCTGGCCC AGCTTCGAAG CTGCCCCCAG GAGGAGGGCC CCAGGGGGCG GGGCTTGGCT
    GCTAGGCCCC TTGAAAACAG AGCAGGGGGG CCTGTGGCAC GTTCAGAGGA GCCTGGTGCC
    CCGCTGCCCG TGGCCGTCGG CACTGCCGAG CCAGGGGGCA GTATGAAGAC CACATTCACC
    ATCGAGATCA AGGACGGCCG TGGCCAGGCC TCCACAGGCC GGGTGCTGCT GCCCACAGGC
    AACCAGAGGG CAGAACTGAC ACTGGGGCTG CGGGCGCCCC CGACCCTACT CAGCACCAGT
    AGTGGGGGCA AGAGCACCAT CACCCGTGTC AACAGCCCTG GGACCCTGGC TCGGCTGGGC
    AGTGTCACTC ATGTCACCAG CTTCAGCCAT GCCCCCCCCA GTAGCCGAGG AGGCTGCAGC
    ATCAAGGCTG CCGAGGATGC CGGGACCCCC GTGGCCCACC CACCTGCCTT CAGCACCCGC
    CGCCGCTCCT CCACCGGCAC CACCCGCAGC ACTAGTCTCA TGGAAGCAGA GCCAGCAGAG
    CCTCTCGCTG CAGCAGTGGA AGCGGCCAAT GGGGCTGAGC AGACCCGAGT GAACAAAGCA
    CCAGAAGGGC GGAGCCCTCT GAGCGCTGAG GAGCTGATGA CTATTGAGGA TGAAGGAGTC
    TTGGACAAGA TGCTGGATCA GAGCACGGAC TTTGAAGAGC GGAAGCTCAT CCGGGCTGCA
    CTTCGTGAGC TCCGACAAAG GAAGAGAGAC CAGCGGGACA AGGAGCGGGA ACGGCGGCTG
    CAGGAGGCAC GGGGCCGGCC AGGGGAGGGG CGCGGCAACA CAGCCACTGA GACCACCACG
    AGGCACAGCC AGCGGGCAGC TGATGGCTCT GCTGTCAGCA CTGTTACCAA GACTGAGCGG
    CTCGTCCACT CCAATGATGG CACACGGACG GCCCGCACCA CCACAGTGGA GTCGAGTTTC
    GTGAGGCGCT CGGAGAATGG CAGTGGCAGC ACCATGATGC AAACCAAGAC CTTCTCCTCT
    TCCTCCTCAT CCAAGAAGAT GGGCAGCATC TTCGACCGCG AGGACCAGGC CAGCCCACGG
    GCCGGCAGCC TGGCGGCGCT CGAGAAACGG CAGGCCGAGA AGAAGAAAGA GCTGATGAAG
    GCGCAGAGTC TGCCCAAGAC CTCAGCCTCC CAGGCGCGCA AGGCCATGAT TGAGAAGCTG
    GAGAAGGAGG GCGCGGCCGG CAGCCCTGGC GGACCCCGCG CAGCCGTGCA GCGATCCACC
    AGCTTCGGGG TCCCCAACGC CAACAGCATC AAGCAGATGC TGCTGGACTG GTGTCGAGCC
    AAGACTCGCG GCTACGAGCA CGTCGACATC CAGAACTTCT CCTCCAGCTG GAGTGATGGG
    ATGGCCTTCT GTGCCCTGGT GCACAACTTC TTCCCTGAGG CCTTCGACTA TGGGCAGCTT
    AGCCCTCAGA ACCGACGCCA GAACTTCGAG GTGGCCTTCT CATCTGCGGA GATGCTGGTG
    GACTGTGTGC CCCTGGTGGA GGTGGACGAC ATGATGATCA TGGGCAAGAA GCCTGACCCC
    AAGTGTGTCT TCACCTATGT GCAGTCGCTC TACAACCACC TGCGACGCCA CGAACTGCGC
    CTGCGCGGCA AGAATGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14920
    Clone ID Related Accession (Same CDS sequence) NM_134270.2 , NM_134270.3
    Accession Version NM_134270.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2823bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-24
    Organism Homo sapiens(human)
    Product smoothelin isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC342566.1, BC034237.2 and Z49989.2. On May 18, 2011 this sequence version replaced NM_134270.1. Transcript Variant: This variant (1) has an alternate 5' exon, which results in a downstream AUG start codon, lacks an in-frame exon in the middle region and has an additional in-frame exon in the 3' region, compared to variant 4. The resulting isoform (a, also referred to as B3) is shorter at the N-terminus, lacks a segment in the middle region and has an additional segment in the C-terminal region, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY061972.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGACCCAT GCGGACTGCC CGCAGCTCCT GGATACAGAG
    GACATGGTGC GGCTTCGAGA GCCTGACTGG AAGATGCTGG TGGACTGTGT GCCCCTGGTG
    GAGGTGGACG ACATGATGAT CATGGGCAAG AAGCCTGACC CCAAGTGTGT CTTCACCTAT
    GTGCAGTCGC TCTACAACCA CCTGCGACGC CACGAACTGC GCCTGCGCGG CAAGAATGTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_599032.2
    CDS242..3064
    Translation

    Target ORF information:

    RefSeq Version NM_134270.2
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_134270.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGACCCAT GCGGACTGCC CGCAGCTCCT GGATACAGAG
    GACATGGTGC GGCTTCGAGA GCCTGACTGG AAGATGCTGG TGGACTGTGT GCCCCTGGTG
    GAGGTGGACG ACATGATGAT CATGGGCAAG AAGCCTGACC CCAAGTGTGT CTTCACCTAT
    GTGCAGTCGC TCTACAACCA CCTGCGACGC CACGAACTGC GCCTGCGCGG CAAGAATGTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14920
    Clone ID Related Accession (Same CDS sequence) NM_134270.2 , NM_134270.3
    Accession Version NM_134270.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2823bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product smoothelin isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC342566.1, BC034237.2 and Z49989.2. On Jun 2, 2019 this sequence version replaced NM_134270.2. Transcript Variant: This variant (1) has an alternate 5' exon, which results in a downstream AUG start codon, lacks an in-frame exon in the middle region and has an additional in-frame exon in the 3' region, compared to variant 4. The resulting isoform (a, also referred to as B3) is shorter at the N-terminus, lacks a segment in the middle region and has an additional segment in the C-terminal region, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY061972.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGACCCAT GCGGACTGCC CGCAGCTCCT GGATACAGAG
    GACATGGTGC GGCTTCGAGA GCCTGACTGG AAGATGCTGG TGGACTGTGT GCCCCTGGTG
    GAGGTGGACG ACATGATGAT CATGGGCAAG AAGCCTGACC CCAAGTGTGT CTTCACCTAT
    GTGCAGTCGC TCTACAACCA CCTGCGACGC CACGAACTGC GCCTGCGCGG CAAGAATGTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_599032.2
    CDS220..3042
    Translation

    Target ORF information:

    RefSeq Version NM_134270.3
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_134270.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGACCCAT GCGGACTGCC CGCAGCTCCT GGATACAGAG
    GACATGGTGC GGCTTCGAGA GCCTGACTGG AAGATGCTGG TGGACTGTGT GCCCCTGGTG
    GAGGTGGACG ACATGATGAT CATGGGCAAG AAGCCTGACC CCAAGTGTGT CTTCACCTAT
    GTGCAGTCGC TCTACAACCA CCTGCGACGC CACGAACTGC GCCTGCGCGG CAAGAATGTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15270
    Clone ID Related Accession (Same CDS sequence) NM_001207018.1
    Accession Version NM_001207018.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2916bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Homo sapiens(human)
    Product smoothelin isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK295418.1, Z49989.2 and BC034237.2. Transcript Variant: This variant (5) lacks two exons from the 5' end, has an alternate 5' exon, lacks an in-frame exon in the middle region and has an additional exon in the 3' region, compared to variant 4. The resulting isoform (e) has distinct N- and C-termini, and lacks an internal segment, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2153733 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGAGGAA TGGGGACGCC TGGGGACTTG CACGCCGCGT GCCCCTCCAC CACCCGCCGG 
    GACGCCCTCT GCCTCGGTCC CGAGTTCGAG TTCGACCTCC GCCACTCAGC TGGGTGTCCT
    GGGGACCTTG GTTTCTTCCC TTTAGATCCG GACACTGAAG CAGGCGGTAG CGGGTGTCTT
    CCTACCTGGC TACCCCTTCG GCGCCTAGCG CGAGGCAGGC TGGAGGTCAC AGCAGATCTG
    GCAGAGCGGC GGCGCATCCG CTCAGCCATC CGGGAACTGC AGCGGCAGGA GCTGGAGCGC
    GAGGAGGAGG CCCTGGCATC CAAGCGTTTC CGTGCCGAGC GGCAGGACAA CAAGGAGAAC
    TGGCTGCACT CTCAGCAGCG GGAAGCTGAG CAGCGGGCTG CCCTGGCACG GCTGGCAGGG
    CAGCTGGAGT CCATGAACGA TGTGGAGGAA TTGACTGCAC TGTTGCGAAG CGCTGGTGAG
    TATGAGGAGC GCAAGCTGAT CCGAGCTGCC ATCCGCCGTG TACGGGCTCA GGAGATTGAG
    GCTGCCACCT TGGCTGGGAG GTTGTACAGC GGGCGTCCCA ACAGTGGCTC AAGAGAGGAC
    AGCAAGGGGC TAGCGGCACA CAGGCTGGAA CAGTGTGAGG TGCCAGAGCG AGAGGAACAG
    GAACAGCAGG CAGAGGTTTC AAAGCCAACC CCCACCCCTG AAGGCACCAG CCAGGATGTG
    ACCACAGTGA CACTCCTGCT GCGAGCCCCA CCTGGGAGCA CATCCAGCTC ACCTGCCTCA
    CCCAGCAGTT CACCCACCCC TGCCTCTCCT GAGCCTCCAT TGGAGCCTGC CGAGGCCCAG
    TGCCTTACAG CTGAGGTTCC AGGCAGCCCA GAGCCACCCC CCAGCCCACC CAAGACCACC
    AGCCCTGAGC CTCAGGAGTC TCCAACGCTC CCCAGCACTG AGGGCCAGGT GGTCAACAAG
    CTTCTGTCTG GCCCCAAAGA GACCCCTGCT GCCCAGAGCC CCACCAGAGG CCCCTCTGAC
    ACCAAGAGAG CAGACGTGGC TGGACCCCGA CCCTGCCAAC GCTCCCTGTC GGTGCTCAGC
    CCCCGCCAAC CAGCCCAGAA CCGAGAGTCC ACCCCCCTTG CCAGCGGACC TTCCTCATTC
    CAGCGGGCTG GCTCTGTGCG GGATCGTGTC CACAAGTTCA CATCTGATTC TCCTATGGCT
    GCTAGGCTCC AGGATGGCAC ACCCCAGGCT GCCCTAAGTC CCCTGACCCC CGCAAGGCTC
    CTGGGCCCCT CCCTCACCAG CACCACCCCT GCCTCCTCCT CCAGCGGCTC CTCCTCTCGG
    GGCCCCAGTG ATACCTCCTC CCGGTTCAGC AAGGAGCAAC GAGGAGTAGC CCAGCCCCTG
    GCCCAGCTTC GAAGCTGCCC CCAGGAGGAG GGCCCCAGGG GGCGGGGCTT GGCTGCTAGG
    CCCCTTGAAA ACAGAGCAGG GGGGCCTGTG GCACGTTCAG AGGAGCCTGG TGCCCCGCTG
    CCCGTGGCCG TCGGCACTGC CGAGCCAGGG GGCAGTATGA AGACCACATT CACCATCGAG
    ATCAAGGACG GCCGTGGCCA GGCCTCCACA GGCCGGGTGC TGCTGCCCAC AGGCAACCAG
    AGGGCAGAAC TGACACTGGG GCTGCGGGCG CCCCCGACCC TACTCAGCAC CAGTAGTGGG
    GGCAAGAGCA CCATCACCCG TGTCAACAGC CCTGGGACCC TGGCTCGGCT GGGCAGTGTC
    ACTCATGTCA CCAGCTTCAG CCATGCCCCC CCCAGTAGCC GAGGAGGCTG CAGCATCAAG
    ATGGAAGCAG AGCCAGCAGA GCCTCTCGCT GCAGCAGTGG AAGCGGCCAA TGGGGCTGAG
    CAGACCCGAG TGAACAAAGC ACCAGAAGGG CGGAGCCCTC TGAGCGCTGA GGAGCTGATG
    ACTATTGAGG ATGAAGGAGT CTTGGACAAG ATGCTGGATC AGAGCACGGA CTTTGAAGAG
    CGGAAGCTCA TCCGGGCTGC ACTTCGTGAG CTCCGACAAA GGAAGAGAGA CCAGCGGGAC
    AAGGAGCGGG AACGGCGGCT GCAGGAGGCA CGGGGCCGGC CAGGGGAGGG GCGCGGCAAC
    ACAGCCACTG AGACCACCAC GAGGCACAGC CAGCGGGCAG CTGATGGCTC TGCTGTCAGC
    ACTGTTACCA AGACTGAGCG GCTCGTCCAC TCCAATGATG GCACACGGAC GGCCCGCACC
    ACCACAGTGG AGTCGAGTTT CGTGAGGCGC TCGGAGAATG GCAGTGGCAG CACCATGATG
    CAAACCAAGA CCTTCTCCTC TTCCTCCTCA TCCAAGAAGA TGGGCAGCAT CTTCGACCGC
    GAGGACCAGG CCAGCCCACG GGCCGGCAGC CTGGCGGCGC TCGAGAAACG GCAGGCCGAG
    AAGAAGAAAG AGCTGATGAA GGCGCAGAGT CTGCCCAAGA CCTCAGCCTC CCAGGCGCGC
    AAGGCCATGA TTGAGAAGCT GGAGAAGGAG GGCGCGGCCG GCAGCCCTGG CGGACCCCGC
    GCAGCCGTGC AGCGATCCAC CAGCTTCGGG GTCCCCAACG CCAACAGCAT CAAGCAGATG
    CTGCTGGACT GGTGTCGAGC CAAGACTCGC GGCTACGAGC ACGTCGACAT CCAGAACTTC
    TCCTCCAGCT GGAGTGATGG GATGGCCTTC TGTGCCCTGG TGCACAACTT CTTCCCTGAG
    GCCTTCGACT ATGGGCAGCT TAGCCCTCAG AACCGACGCC AGAACTTCGA GGTGGCCTTC
    TCATCTGCGG AGACCCATGC GGACTGCCCG CAGCTCCTGG ATACAGAGGA CATGGTGCGG
    CTTCGAGAGC CTGACTGGAA GTGCGTGTAC ACGTACATCC AGGAATTCTA CCGCTGTCTG
    GTCCAGAAGG GGCTGGTAAA AACCAAAAAG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193947.1
    CDS78..2993
    Translation

    Target ORF information:

    RefSeq Version NM_001207018.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001207018.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGAGGAA TGGGGACGCC TGGGGACTTG CACGCCGCGT GCCCCTCCAC CACCCGCCGG 
    GACGCCCTCT GCCTCGGTCC CGAGTTCGAG TTCGACCTCC GCCACTCAGC TGGGTGTCCT
    GGGGACCTTG GTTTCTTCCC TTTAGATCCG GACACTGAAG CAGGCGGTAG CGGGTGTCTT
    CCTACCTGGC TACCCCTTCG GCGCCTAGCG CGAGGCAGGC TGGAGGTCAC AGCAGATCTG
    GCAGAGCGGC GGCGCATCCG CTCAGCCATC CGGGAACTGC AGCGGCAGGA GCTGGAGCGC
    GAGGAGGAGG CCCTGGCATC CAAGCGTTTC CGTGCCGAGC GGCAGGACAA CAAGGAGAAC
    TGGCTGCACT CTCAGCAGCG GGAAGCTGAG CAGCGGGCTG CCCTGGCACG GCTGGCAGGG
    CAGCTGGAGT CCATGAACGA TGTGGAGGAA TTGACTGCAC TGTTGCGAAG CGCTGGTGAG
    TATGAGGAGC GCAAGCTGAT CCGAGCTGCC ATCCGCCGTG TACGGGCTCA GGAGATTGAG
    GCTGCCACCT TGGCTGGGAG GTTGTACAGC GGGCGTCCCA ACAGTGGCTC AAGAGAGGAC
    AGCAAGGGGC TAGCGGCACA CAGGCTGGAA CAGTGTGAGG TGCCAGAGCG AGAGGAACAG
    GAACAGCAGG CAGAGGTTTC AAAGCCAACC CCCACCCCTG AAGGCACCAG CCAGGATGTG
    ACCACAGTGA CACTCCTGCT GCGAGCCCCA CCTGGGAGCA CATCCAGCTC ACCTGCCTCA
    CCCAGCAGTT CACCCACCCC TGCCTCTCCT GAGCCTCCAT TGGAGCCTGC CGAGGCCCAG
    TGCCTTACAG CTGAGGTTCC AGGCAGCCCA GAGCCACCCC CCAGCCCACC CAAGACCACC
    AGCCCTGAGC CTCAGGAGTC TCCAACGCTC CCCAGCACTG AGGGCCAGGT GGTCAACAAG
    CTTCTGTCTG GCCCCAAAGA GACCCCTGCT GCCCAGAGCC CCACCAGAGG CCCCTCTGAC
    ACCAAGAGAG CAGACGTGGC TGGACCCCGA CCCTGCCAAC GCTCCCTGTC GGTGCTCAGC
    CCCCGCCAAC CAGCCCAGAA CCGAGAGTCC ACCCCCCTTG CCAGCGGACC TTCCTCATTC
    CAGCGGGCTG GCTCTGTGCG GGATCGTGTC CACAAGTTCA CATCTGATTC TCCTATGGCT
    GCTAGGCTCC AGGATGGCAC ACCCCAGGCT GCCCTAAGTC CCCTGACCCC CGCAAGGCTC
    CTGGGCCCCT CCCTCACCAG CACCACCCCT GCCTCCTCCT CCAGCGGCTC CTCCTCTCGG
    GGCCCCAGTG ATACCTCCTC CCGGTTCAGC AAGGAGCAAC GAGGAGTAGC CCAGCCCCTG
    GCCCAGCTTC GAAGCTGCCC CCAGGAGGAG GGCCCCAGGG GGCGGGGCTT GGCTGCTAGG
    CCCCTTGAAA ACAGAGCAGG GGGGCCTGTG GCACGTTCAG AGGAGCCTGG TGCCCCGCTG
    CCCGTGGCCG TCGGCACTGC CGAGCCAGGG GGCAGTATGA AGACCACATT CACCATCGAG
    ATCAAGGACG GCCGTGGCCA GGCCTCCACA GGCCGGGTGC TGCTGCCCAC AGGCAACCAG
    AGGGCAGAAC TGACACTGGG GCTGCGGGCG CCCCCGACCC TACTCAGCAC CAGTAGTGGG
    GGCAAGAGCA CCATCACCCG TGTCAACAGC CCTGGGACCC TGGCTCGGCT GGGCAGTGTC
    ACTCATGTCA CCAGCTTCAG CCATGCCCCC CCCAGTAGCC GAGGAGGCTG CAGCATCAAG
    ATGGAAGCAG AGCCAGCAGA GCCTCTCGCT GCAGCAGTGG AAGCGGCCAA TGGGGCTGAG
    CAGACCCGAG TGAACAAAGC ACCAGAAGGG CGGAGCCCTC TGAGCGCTGA GGAGCTGATG
    ACTATTGAGG ATGAAGGAGT CTTGGACAAG ATGCTGGATC AGAGCACGGA CTTTGAAGAG
    CGGAAGCTCA TCCGGGCTGC ACTTCGTGAG CTCCGACAAA GGAAGAGAGA CCAGCGGGAC
    AAGGAGCGGG AACGGCGGCT GCAGGAGGCA CGGGGCCGGC CAGGGGAGGG GCGCGGCAAC
    ACAGCCACTG AGACCACCAC GAGGCACAGC CAGCGGGCAG CTGATGGCTC TGCTGTCAGC
    ACTGTTACCA AGACTGAGCG GCTCGTCCAC TCCAATGATG GCACACGGAC GGCCCGCACC
    ACCACAGTGG AGTCGAGTTT CGTGAGGCGC TCGGAGAATG GCAGTGGCAG CACCATGATG
    CAAACCAAGA CCTTCTCCTC TTCCTCCTCA TCCAAGAAGA TGGGCAGCAT CTTCGACCGC
    GAGGACCAGG CCAGCCCACG GGCCGGCAGC CTGGCGGCGC TCGAGAAACG GCAGGCCGAG
    AAGAAGAAAG AGCTGATGAA GGCGCAGAGT CTGCCCAAGA CCTCAGCCTC CCAGGCGCGC
    AAGGCCATGA TTGAGAAGCT GGAGAAGGAG GGCGCGGCCG GCAGCCCTGG CGGACCCCGC
    GCAGCCGTGC AGCGATCCAC CAGCTTCGGG GTCCCCAACG CCAACAGCAT CAAGCAGATG
    CTGCTGGACT GGTGTCGAGC CAAGACTCGC GGCTACGAGC ACGTCGACAT CCAGAACTTC
    TCCTCCAGCT GGAGTGATGG GATGGCCTTC TGTGCCCTGG TGCACAACTT CTTCCCTGAG
    GCCTTCGACT ATGGGCAGCT TAGCCCTCAG AACCGACGCC AGAACTTCGA GGTGGCCTTC
    TCATCTGCGG AGACCCATGC GGACTGCCCG CAGCTCCTGG ATACAGAGGA CATGGTGCGG
    CTTCGAGAGC CTGACTGGAA GTGCGTGTAC ACGTACATCC AGGAATTCTA CCGCTGTCTG
    GTCCAGAAGG GGCTGGTAAA AACCAAAAAG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18905
    Clone ID Related Accession (Same CDS sequence) NM_134269.2 , NM_134269.3
    Accession Version NM_134269.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-24
    Organism Homo sapiens(human)
    Product smoothelin isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC342566.1, BC034237.2 and Z49989.2. This sequence is a reference standard in the RefSeqGene project. On May 18, 2011 this sequence version replaced NM_134269.1. Transcript Variant: This variant (2) has an alternate 5' exon, which results in a downstream AUG start codon, lacks an in-frame exon in the middle region and has an additional exon in the 3' region, compared to variant 4. The resulting isoform (b, also referred to as L2 and B2) is shorter at the N-terminus, lacks a segment in the middle region and has a slightly shorter and distinct C-terminus, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF064238.3, AY061971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGACCCAT GCGGACTGCC CGCAGCTCCT GGATACAGAG
    GACATGGTGC GGCTTCGAGA GCCTGACTGG AAGTGCGTGT ACACGTACAT CCAGGAATTC
    TACCGCTGTC TGGTCCAGAA GGGGCTGGTA AAAACCAAAA AGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_599031.1
    CDS242..2989
    Translation

    Target ORF information:

    RefSeq Version NM_134269.2
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_134269.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGACCCAT GCGGACTGCC CGCAGCTCCT GGATACAGAG
    GACATGGTGC GGCTTCGAGA GCCTGACTGG AAGTGCGTGT ACACGTACAT CCAGGAATTC
    TACCGCTGTC TGGTCCAGAA GGGGCTGGTA AAAACCAAAA AGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18905
    Clone ID Related Accession (Same CDS sequence) NM_134269.2 , NM_134269.3
    Accession Version NM_134269.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product smoothelin isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC342566.1, BC034237.2 and Z49989.2. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_134269.2. Transcript Variant: This variant (2) has an alternate 5' exon, which results in a downstream AUG start codon, lacks an in-frame exon in the middle region and has an additional exon in the 3' region, compared to variant 4. The resulting isoform (b, also referred to as L2 and B2) is shorter at the N-terminus, lacks a segment in the middle region and has a slightly shorter and distinct C-terminus, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1367479.1, BC034237.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGACCCAT GCGGACTGCC CGCAGCTCCT GGATACAGAG
    GACATGGTGC GGCTTCGAGA GCCTGACTGG AAGTGCGTGT ACACGTACAT CCAGGAATTC
    TACCGCTGTC TGGTCCAGAA GGGGCTGGTA AAAACCAAAA AGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_599031.1
    CDS220..2967
    Translation

    Target ORF information:

    RefSeq Version NM_134269.3
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_134269.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGATGGAAGC AGAGCCAGCA GAGCCTCTCG CTGCAGCAGT GGAAGCGGCC
    AATGGGGCTG AGCAGACCCG AGTGAACAAA GCACCAGAAG GGCGGAGCCC TCTGAGCGCT
    GAGGAGCTGA TGACTATTGA GGATGAAGGA GTCTTGGACA AGATGCTGGA TCAGAGCACG
    GACTTTGAAG AGCGGAAGCT CATCCGGGCT GCACTTCGTG AGCTCCGACA AAGGAAGAGA
    GACCAGCGGG ACAAGGAGCG GGAACGGCGG CTGCAGGAGG CACGGGGCCG GCCAGGGGAG
    GGGCGCGGCA ACACAGCCAC TGAGACCACC ACGAGGCACA GCCAGCGGGC AGCTGATGGC
    TCTGCTGTCA GCACTGTTAC CAAGACTGAG CGGCTCGTCC ACTCCAATGA TGGCACACGG
    ACGGCCCGCA CCACCACAGT GGAGTCGAGT TTCGTGAGGC GCTCGGAGAA TGGCAGTGGC
    AGCACCATGA TGCAAACCAA GACCTTCTCC TCTTCCTCCT CATCCAAGAA GATGGGCAGC
    ATCTTCGACC GCGAGGACCA GGCCAGCCCA CGGGCCGGCA GCCTGGCGGC GCTCGAGAAA
    CGGCAGGCCG AGAAGAAGAA AGAGCTGATG AAGGCGCAGA GTCTGCCCAA GACCTCAGCC
    TCCCAGGCGC GCAAGGCCAT GATTGAGAAG CTGGAGAAGG AGGGCGCGGC CGGCAGCCCT
    GGCGGACCCC GCGCAGCCGT GCAGCGATCC ACCAGCTTCG GGGTCCCCAA CGCCAACAGC
    ATCAAGCAGA TGCTGCTGGA CTGGTGTCGA GCCAAGACTC GCGGCTACGA GCACGTCGAC
    ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCCT TCTGTGCCCT GGTGCACAAC
    TTCTTCCCTG AGGCCTTCGA CTATGGGCAG CTTAGCCCTC AGAACCGACG CCAGAACTTC
    GAGGTGGCCT TCTCATCTGC GGAGACCCAT GCGGACTGCC CGCAGCTCCT GGATACAGAG
    GACATGGTGC GGCTTCGAGA GCCTGACTGG AAGTGCGTGT ACACGTACAT CCAGGAATTC
    TACCGCTGTC TGGTCCAGAA GGGGCTGGTA AAAACCAAAA AGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53659
    Clone ID Related Accession (Same CDS sequence) XM_006724298.1
    Accession Version XM_006724298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2847bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGGCTGCCGA GGATGCCGGG ACCCCCGTGG CCCACCCACC TGCCTTCAGC
    ACCCGCCGCC GCTCCTCCAC CGGCACCACC CGCAGCACTA GTCTCATGGA AGCAGAGCCA
    GCAGAGCCTC TCGCTGCAGC AGTGGAAGCG GCCAATGGGG CTGAGCAGAC CCGAGTGAAC
    AAAGCACCAG AAGGGCGGAG CCCTCTGAGC GCTGAGGAGC TGATGACTAT TGAGGATGAA
    GGAGTCTTGG ACAAGATGCT GGATCAGAGC ACGGACTTTG AAGAGCGGAA GCTCATCCGG
    GCTGCACTTC GTGAGCTCCG ACAAAGGAAG AGAGACCAGC GGGACAAGGA GCGGGAACGG
    CGGCTGCAGG AGGCACGGGG CCGGCCAGGG GAGGGGCGCG GCAACACAGC CACTGAGACC
    ACCACGAGGC ACAGCCAGCG GGCAGCTGAT GGCTCTGCTG TCAGCACTGT TACCAAGACT
    GAGCGGCTCG TCCACTCCAA TGATGGCACA CGGACGGCCC GCACCACCAC AGTGGAGTCG
    AGTTTCGTGA GGCGCTCGGA GAATGGCAGT GGCAGCACCA TGATGCAAAC CAAGACCTTC
    TCCTCTTCCT CCTCATCCAA GAAGATGGGC AGCATCTTCG ACCGCGAGGA CCAGGCCAGC
    CCACGGGCCG GCAGCCTGGC GGCGCTCGAG AAACGGCAGG CCGAGAAGAA GAAAGAGCTG
    ATGAAGGCGC AGAGTCTGCC CAAGACCTCA GCCTCCCAGG CGCGCAAGGC CATGATTGAG
    AAGCTGGAGA AGGAGGGCGC GGCCGGCAGC CCTGGCGGAC CCCGCGCAGC CGTGCAGCGA
    TCCACCAGCT TCGGGGTCCC CAACGCCAAC AGCATCAAGC AGATGCTGCT GGACTGGTGT
    CGAGCCAAGA CTCGCGGCTA CGAGCACGTC GACATCCAGA ACTTCTCCTC CAGCTGGAGT
    GATGGGATGG CCTTCTGTGC CCTGGTGCAC AACTTCTTCC CTGAGGCCTT CGACTATGGG
    CAGCTTAGCC CTCAGAACCG ACGCCAGAAC TTCGAGGTGG CCTTCTCATC TGCGGAGATG
    CTGGTGGACT GTGTGCCCCT GGTGGAGGTG GACGACATGA TGATCATGGG CAAGAAGCCT
    GACCCCAAGT GTGTCTTCAC CTATGTGCAG TCGCTCTACA ACCACCTGCG ACGCCACGAA
    CTGCGCCTGC GCGGCAAGAA TGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006724361.1
    CDS220..3066
    Translation

    Target ORF information:

    RefSeq Version XM_006724298.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006724298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGACG AGGCCTTAGC TGGGCTGGAT GAGGGAGCCC TTCGGAAGCT GCTGGAGGTC 
    ACAGCAGATC TGGCAGAGCG GCGGCGCATC CGCTCAGCCA TCCGGGAACT GCAGCGGCAG
    GAGCTGGAGC GCGAGGAGGA GGCCCTGGCA TCCAAGCGTT TCCGTGCCGA GCGGCAGGAC
    AACAAGGAGA ACTGGCTGCA CTCTCAGCAG CGGGAAGCTG AGCAGCGGGC TGCCCTGGCA
    CGGCTGGCAG GGCAGCTGGA GTCCATGAAC GATGTGGAGG AATTGACTGC ACTGTTGCGA
    AGCGCTGGTG AGTATGAGGA GCGCAAGCTG ATCCGAGCTG CCATCCGCCG TGTACGGGCT
    CAGGAGATTG AGGCTGCCAC CTTGGCTGGG AGGTTGTACA GCGGGCGTCC CAACAGTGGC
    TCAAGAGAGG ACAGCAAGGG GCTAGCGGCA CACAGGCTGG AACAGTGTGA GGTGCCAGAG
    CGAGAGGAAC AGGAACAGCA GGCAGAGGTT TCAAAGCCAA CCCCCACCCC TGAAGGCACC
    AGCCAGGATG TGACCACAGT GACACTCCTG CTGCGAGCCC CACCTGGGAG CACATCCAGC
    TCACCTGCCT CACCCAGCAG TTCACCCACC CCTGCCTCTC CTGAGCCTCC ATTGGAGCCT
    GCCGAGGCCC AGTGCCTTAC AGCTGAGGTT CCAGGCAGCC CAGAGCCACC CCCCAGCCCA
    CCCAAGACCA CCAGCCCTGA GCCTCAGGAG TCTCCAACGC TCCCCAGCAC TGAGGGCCAG
    GTGGTCAACA AGCTTCTGTC TGGCCCCAAA GAGACCCCTG CTGCCCAGAG CCCCACCAGA
    GGCCCCTCTG ACACCAAGAG AGCAGACGTG GCTGGACCCC GACCCTGCCA ACGCTCCCTG
    TCGGTGCTCA GCCCCCGCCA ACCAGCCCAG AACCGAGAGT CCACCCCCCT TGCCAGCGGA
    CCTTCCTCAT TCCAGCGGGC TGGCTCTGTG CGGGATCGTG TCCACAAGTT CACATCTGAT
    TCTCCTATGG CTGCTAGGCT CCAGGATGGC ACACCCCAGG CTGCCCTAAG TCCCCTGACC
    CCCGCAAGGC TCCTGGGCCC CTCCCTCACC AGCACCACCC CTGCCTCCTC CTCCAGCGGC
    TCCTCCTCTC GGGGCCCCAG TGATACCTCC TCCCGGTTCA GCAAGGAGCA ACGAGGAGTA
    GCCCAGCCCC TGGCCCAGCT TCGAAGCTGC CCCCAGGAGG AGGGCCCCAG GGGGCGGGGC
    TTGGCTGCTA GGCCCCTTGA AAACAGAGCA GGGGGGCCTG TGGCACGTTC AGAGGAGCCT
    GGTGCCCCGC TGCCCGTGGC CGTCGGCACT GCCGAGCCAG GGGGCAGTAT GAAGACCACA
    TTCACCATCG AGATCAAGGA CGGCCGTGGC CAGGCCTCCA CAGGCCGGGT GCTGCTGCCC
    ACAGGCAACC AGAGGGCAGA ACTGACACTG GGGCTGCGGG CGCCCCCGAC CCTACTCAGC
    ACCAGTAGTG GGGGCAAGAG CACCATCACC CGTGTCAACA GCCCTGGGAC CCTGGCTCGG
    CTGGGCAGTG TCACTCATGT CACCAGCTTC AGCCATGCCC CCCCCAGTAG CCGAGGAGGC
    TGCAGCATCA AGGCTGCCGA GGATGCCGGG ACCCCCGTGG CCCACCCACC TGCCTTCAGC
    ACCCGCCGCC GCTCCTCCAC CGGCACCACC CGCAGCACTA GTCTCATGGA AGCAGAGCCA
    GCAGAGCCTC TCGCTGCAGC AGTGGAAGCG GCCAATGGGG CTGAGCAGAC CCGAGTGAAC
    AAAGCACCAG AAGGGCGGAG CCCTCTGAGC GCTGAGGAGC TGATGACTAT TGAGGATGAA
    GGAGTCTTGG ACAAGATGCT GGATCAGAGC ACGGACTTTG AAGAGCGGAA GCTCATCCGG
    GCTGCACTTC GTGAGCTCCG ACAAAGGAAG AGAGACCAGC GGGACAAGGA GCGGGAACGG
    CGGCTGCAGG AGGCACGGGG CCGGCCAGGG GAGGGGCGCG GCAACACAGC CACTGAGACC
    ACCACGAGGC ACAGCCAGCG GGCAGCTGAT GGCTCTGCTG TCAGCACTGT TACCAAGACT
    GAGCGGCTCG TCCACTCCAA TGATGGCACA CGGACGGCCC GCACCACCAC AGTGGAGTCG
    AGTTTCGTGA GGCGCTCGGA GAATGGCAGT GGCAGCACCA TGATGCAAAC CAAGACCTTC
    TCCTCTTCCT CCTCATCCAA GAAGATGGGC AGCATCTTCG ACCGCGAGGA CCAGGCCAGC
    CCACGGGCCG GCAGCCTGGC GGCGCTCGAG AAACGGCAGG CCGAGAAGAA GAAAGAGCTG
    ATGAAGGCGC AGAGTCTGCC CAAGACCTCA GCCTCCCAGG CGCGCAAGGC CATGATTGAG
    AAGCTGGAGA AGGAGGGCGC GGCCGGCAGC CCTGGCGGAC CCCGCGCAGC CGTGCAGCGA
    TCCACCAGCT TCGGGGTCCC CAACGCCAAC AGCATCAAGC AGATGCTGCT GGACTGGTGT
    CGAGCCAAGA CTCGCGGCTA CGAGCACGTC GACATCCAGA ACTTCTCCTC CAGCTGGAGT
    GATGGGATGG CCTTCTGTGC CCTGGTGCAC AACTTCTTCC CTGAGGCCTT CGACTATGGG
    CAGCTTAGCC CTCAGAACCG ACGCCAGAAC TTCGAGGTGG CCTTCTCATC TGCGGAGATG
    CTGGTGGACT GTGTGCCCCT GGTGGAGGTG GACGACATGA TGATCATGGG CAAGAAGCCT
    GACCCCAAGT GTGTCTTCAC CTATGTGCAG TCGCTCTACA ACCACCTGCG ACGCCACGAA
    CTGCGCCTGC GCGGCAAGAA TGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53663
    Clone ID Related Accession (Same CDS sequence) XM_006724302.1
    Accession Version XM_006724302.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAACGATG TGGAGGAATT GACTGCACTG TTGCGAAGCG CTGGTGAGTA TGAGGAGCGC 
    AAGCTGATCC GAGCTGCCAT CCGCCGTGTA CGGGCTCAGG AGATTGAGGC TGCCACCTTG
    GCTGGGAGGT TGTACAGCGG GCGTCCCAAC AGTGGCTCAA GAGAGGACAG CAAGGGGCTA
    GCGGCACACA GGCTGGAACA GTGTGAGGTG CCAGAGCGAG AGGAACAGGA ACAGCAGGCA
    GAGGTTTCAA AGCCAACCCC CACCCCTGAA GGCACCAGCC AGGATGTGAC CACAGTGACA
    CTCCTGCTGC GAGCCCCACC TGGGAGCACA TCCAGCTCAC CTGCCTCACC CAGCAGTTCA
    CCCACCCCTG CCTCTCCTGA GCCTCCATTG GAGCCTGCCG AGGCCCAGTG CCTTACAGCT
    GAGGTTCCAG GCAGCCCAGA GCCACCCCCC AGCCCACCCA AGACCACCAG CCCTGAGCCT
    CAGGAGTCTC CAACGCTCCC CAGCACTGAG GGCCAGGTGG TCAACAAGCT TCTGTCTGGC
    CCCAAAGAGA CCCCTGCTGC CCAGAGCCCC ACCAGAGGCC CCTCTGACAC CAAGAGAGCA
    GACGTGGCTG GACCCCGACC CTGCCAACGC TCCCTGTCGG TGCTCAGCCC CCGCCAACCA
    GCCCAGAACC GAGAGTCCAC CCCCCTTGCC AGCGGACCTT CCTCATTCCA GCGGGCTGGC
    TCTGTGCGGG ATCGTGTCCA CAAGTTCACA TCTGATTCTC CTATGGCTGC TAGGCTCCAG
    GATGGCACAC CCCAGGCTGC CCTAAGTCCC CTGACCCCCG CAAGGCTCCT GGGCCCCTCC
    CTCACCAGCA CCACCCCTGC CTCCTCCTCC AGCGGCTCCT CCTCTCGGGG CCCCAGTGAT
    ACCTCCTCCC GGTTCAGCAA GGAGCAACGA GGAGTAGCCC AGCCCCTGGC CCAGCTTCGA
    AGCTGCCCCC AGGAGGAGGG CCCCAGGGGG CGGGGCTTGG CTGCTAGGCC CCTTGAAAAC
    AGAGCAGGGG GGCCTGTGGC ACGTTCAGAG GAGCCTGGTG CCCCGCTGCC CGTGGCCGTC
    GGCACTGCCG AGCCAGGGGG CAGTATGAAG ACCACATTCA CCATCGAGAT CAAGGACGGC
    CGTGGCCAGG CCTCCACAGG CCGGGTGCTG CTGCCCACAG GCAACCAGAG GGCAGAACTG
    ACACTGGGGC TGCGGGCGCC CCCGACCCTA CTCAGCACCA GTAGTGGGGG CAAGAGCACC
    ATCACCCGTG TCAACAGCCC TGGGACCCTG GCTCGGCTGG GCAGTGTCAC TCATGTCACC
    AGCTTCAGCC ATGCCCCCCC CAGTAGCCGA GGAGGCTGCA GCATCAAGGC TGCCGAGGAT
    GCCGGGACCC CCGTGGCCCA CCCACCTGCC TTCAGCACCC GCCGCCGCTC CTCCACCGGC
    ACCACCCGCA GCACTAGTCT CATGGAAGCA GAGCCAGCAG AGCCTCTCGC TGCAGCAGTG
    GAAGCGGCCA ATGGGGCTGA GCAGACCCGA GTGAACAAAG CACCAGAAGG GCGGAGCCCT
    CTGAGCGCTG AGGAGCTGAT GACTATTGAG GATGAAGGAG TCTTGGACAA GATGCTGGAT
    CAGAGCACGG ACTTTGAAGA GCGGAAGCTC ATCCGGGCTG CACTTCGTGA GCTCCGACAA
    AGGAAGAGAG ACCAGCGGGA CAAGGAGCGG GAACGGCGGC TGCAGGAGGC ACGGGGCCGG
    CCAGGGGAGG GGCGCGGCAA CACAGCCACT GAGACCACCA CGAGGCACAG CCAGCGGGCA
    GCTGATGGCT CTGCTGTCAG CACTGTTACC AAGACTGAGC GGCTCGTCCA CTCCAATGAT
    GGCACACGGA CGGCCCGCAC CACCACAGTG GAGTCGAGTT TCGTGAGGCG CTCGGAGAAT
    GGCAGTGGCA GCACCATGAT GCAAACCAAG ACCTTCTCCT CTTCCTCCTC ATCCAAGAAG
    ATGGGCAGCA TCTTCGACCG CGAGGACCAG GCCAGCCCAC GGGCCGGCAG CCTGGCGGCG
    CTCGAGAAAC GGCAGGCCGA GAAGAAGAAA GAGCTGATGA AGGCGCAGAG TCTGCCCAAG
    ACCTCAGCCT CCCAGGCGCG CAAGGCCATG ATTGAGAAGC TGGAGAAGGA GGGCGCGGCC
    GGCAGCCCTG GCGGACCCCG CGCAGCCGTG CAGCGATCCA CCAGCTTCGG GGTCCCCAAC
    GCCAACAGCA TCAAGCAGAT GCTGCTGGAC TGGTGTCGAG CCAAGACTCG CGGCTACGAG
    CACGTCGACA TCCAGAACTT CTCCTCCAGC TGGAGTGATG GGATGGCCTT CTGTGCCCTG
    GTGCACAACT TCTTCCCTGA GGCCTTCGAC TATGGGCAGC TTAGCCCTCA GAACCGACGC
    CAGAACTTCG AGGTGGCCTT CTCATCTGCG GAGATGCTGG TGGACTGTGT GCCCCTGGTG
    GAGGTGGACG ACATGATGAT CATGGGCAAG AAGCCTGACC CCAAGTGTGT CTTCACCTAT
    GTGCAGTCGC TCTACAACCA CCTGCGACGC CACGAACTGC GCCTGCGCGG CAAGAATGTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006724365.1
    CDS543..3125
    Translation

    Target ORF information:

    RefSeq Version XM_006724302.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006724302.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAACGATG TGGAGGAATT GACTGCACTG TTGCGAAGCG CTGGTGAGTA TGAGGAGCGC 
    AAGCTGATCC GAGCTGCCAT CCGCCGTGTA CGGGCTCAGG AGATTGAGGC TGCCACCTTG
    GCTGGGAGGT TGTACAGCGG GCGTCCCAAC AGTGGCTCAA GAGAGGACAG CAAGGGGCTA
    GCGGCACACA GGCTGGAACA GTGTGAGGTG CCAGAGCGAG AGGAACAGGA ACAGCAGGCA
    GAGGTTTCAA AGCCAACCCC CACCCCTGAA GGCACCAGCC AGGATGTGAC CACAGTGACA
    CTCCTGCTGC GAGCCCCACC TGGGAGCACA TCCAGCTCAC CTGCCTCACC CAGCAGTTCA
    CCCACCCCTG CCTCTCCTGA GCCTCCATTG GAGCCTGCCG AGGCCCAGTG CCTTACAGCT
    GAGGTTCCAG GCAGCCCAGA GCCACCCCCC AGCCCACCCA AGACCACCAG CCCTGAGCCT
    CAGGAGTCTC CAACGCTCCC CAGCACTGAG GGCCAGGTGG TCAACAAGCT TCTGTCTGGC
    CCCAAAGAGA CCCCTGCTGC CCAGAGCCCC ACCAGAGGCC CCTCTGACAC CAAGAGAGCA
    GACGTGGCTG GACCCCGACC CTGCCAACGC TCCCTGTCGG TGCTCAGCCC CCGCCAACCA
    GCCCAGAACC GAGAGTCCAC CCCCCTTGCC AGCGGACCTT CCTCATTCCA GCGGGCTGGC
    TCTGTGCGGG ATCGTGTCCA CAAGTTCACA TCTGATTCTC CTATGGCTGC TAGGCTCCAG
    GATGGCACAC CCCAGGCTGC CCTAAGTCCC CTGACCCCCG CAAGGCTCCT GGGCCCCTCC
    CTCACCAGCA CCACCCCTGC CTCCTCCTCC AGCGGCTCCT CCTCTCGGGG CCCCAGTGAT
    ACCTCCTCCC GGTTCAGCAA GGAGCAACGA GGAGTAGCCC AGCCCCTGGC CCAGCTTCGA
    AGCTGCCCCC AGGAGGAGGG CCCCAGGGGG CGGGGCTTGG CTGCTAGGCC CCTTGAAAAC
    AGAGCAGGGG GGCCTGTGGC ACGTTCAGAG GAGCCTGGTG CCCCGCTGCC CGTGGCCGTC
    GGCACTGCCG AGCCAGGGGG CAGTATGAAG ACCACATTCA CCATCGAGAT CAAGGACGGC
    CGTGGCCAGG CCTCCACAGG CCGGGTGCTG CTGCCCACAG GCAACCAGAG GGCAGAACTG
    ACACTGGGGC TGCGGGCGCC CCCGACCCTA CTCAGCACCA GTAGTGGGGG CAAGAGCACC
    ATCACCCGTG TCAACAGCCC TGGGACCCTG GCTCGGCTGG GCAGTGTCAC TCATGTCACC
    AGCTTCAGCC ATGCCCCCCC CAGTAGCCGA GGAGGCTGCA GCATCAAGGC TGCCGAGGAT
    GCCGGGACCC CCGTGGCCCA CCCACCTGCC TTCAGCACCC GCCGCCGCTC CTCCACCGGC
    ACCACCCGCA GCACTAGTCT CATGGAAGCA GAGCCAGCAG AGCCTCTCGC TGCAGCAGTG
    GAAGCGGCCA ATGGGGCTGA GCAGACCCGA GTGAACAAAG CACCAGAAGG GCGGAGCCCT
    CTGAGCGCTG AGGAGCTGAT GACTATTGAG GATGAAGGAG TCTTGGACAA GATGCTGGAT
    CAGAGCACGG ACTTTGAAGA GCGGAAGCTC ATCCGGGCTG CACTTCGTGA GCTCCGACAA
    AGGAAGAGAG ACCAGCGGGA CAAGGAGCGG GAACGGCGGC TGCAGGAGGC ACGGGGCCGG
    CCAGGGGAGG GGCGCGGCAA CACAGCCACT GAGACCACCA CGAGGCACAG CCAGCGGGCA
    GCTGATGGCT CTGCTGTCAG CACTGTTACC AAGACTGAGC GGCTCGTCCA CTCCAATGAT
    GGCACACGGA CGGCCCGCAC CACCACAGTG GAGTCGAGTT TCGTGAGGCG CTCGGAGAAT
    GGCAGTGGCA GCACCATGAT GCAAACCAAG ACCTTCTCCT CTTCCTCCTC ATCCAAGAAG
    ATGGGCAGCA TCTTCGACCG CGAGGACCAG GCCAGCCCAC GGGCCGGCAG CCTGGCGGCG
    CTCGAGAAAC GGCAGGCCGA GAAGAAGAAA GAGCTGATGA AGGCGCAGAG TCTGCCCAAG
    ACCTCAGCCT CCCAGGCGCG CAAGGCCATG ATTGAGAAGC TGGAGAAGGA GGGCGCGGCC
    GGCAGCCCTG GCGGACCCCG CGCAGCCGTG CAGCGATCCA CCAGCTTCGG GGTCCCCAAC
    GCCAACAGCA TCAAGCAGAT GCTGCTGGAC TGGTGTCGAG CCAAGACTCG CGGCTACGAG
    CACGTCGACA TCCAGAACTT CTCCTCCAGC TGGAGTGATG GGATGGCCTT CTGTGCCCTG
    GTGCACAACT TCTTCCCTGA GGCCTTCGAC TATGGGCAGC TTAGCCCTCA GAACCGACGC
    CAGAACTTCG AGGTGGCCTT CTCATCTGCG GAGATGCTGG TGGACTGTGT GCCCCTGGTG
    GAGGTGGACG ACATGATGAT CATGGGCAAG AAGCCTGACC CCAAGTGTGT CTTCACCTAT
    GTGCAGTCGC TCTACAACCA CCTGCGACGC CACGAACTGC GCCTGCGCGG CAAGAATGTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60555
    Clone ID Related Accession (Same CDS sequence) XM_017028914.1
    Accession Version XM_017028914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2916bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGATGGAA
    GCAGAGCCAG CAGAGCCTCT CGCTGCAGCA GTGGAAGCGG CCAATGGGGC TGAGCAGACC
    CGAGTGAACA AAGCACCAGA AGGGCGGAGC CCTCTGAGCG CTGAGGAGCT GATGACTATT
    GAGGATGAAG GAGTCTTGGA CAAGATGCTG GATCAGAGCA CGGACTTTGA AGAGCGGAAG
    CTCATCCGGG CTGCACTTCG TGAGCTCCGA CAAAGGAAGA GAGACCAGCG GGACAAGGAG
    CGGGAACGGC GGCTGCAGGA GGCACGGGGC CGGCCAGGGG AGGGGCGCGG CAACACAGCC
    ACTGAGACCA CCACGAGGCA CAGCCAGCGG GCAGCTGATG GCTCTGCTGT CAGCACTGTT
    ACCAAGACTG AGCGGCTCGT CCACTCCAAT GATGGCACAC GGACGGCCCG CACCACCACA
    GTGGAGTCGA GTTTCGTGAG GCGCTCGGAG AATGGCAGTG GCAGCACCAT GATGCAAACC
    AAGACCTTCT CCTCTTCCTC CTCATCCAAG AAGATGGGCA GCATCTTCGA CCGCGAGGAC
    CAGGCCAGCC CACGGGCCGG CAGCCTGGCG GCGCTCGAGA AACGGCAGGC CGAGAAGAAG
    AAAGAGCTGA TGAAGGCGCA GAGTCTGCCC AAGACCTCAG CCTCCCAGGC GCGCAAGGCC
    ATGATTGAGA AGCTGGAGAA GGAGGGCGCG GCCGGCAGCC CTGGCGGACC CCGCGCAGCC
    GTGCAGCGAT CCACCAGCTT CGGGGTCCCC AACGCCAACA GCATCAAGCA GATGCTGCTG
    GACTGGTGTC GAGCCAAGAC TCGCGGCTAC GAGCACGTCG ACATCCAGAA CTTCTCCTCC
    AGCTGGAGTG ATGGGATGGC CTTCTGTGCC CTGGTGCACA ACTTCTTCCC TGAGGCCTTC
    GACTATGGGC AGCTTAGCCC TCAGAACCGA CGCCAGAACT TCGAGGTGGC CTTCTCATCT
    GCGGAGATGC TGGTGGACTG TGTGCCCCTG GTGGAGGTGG ACGACATGAT GATCATGGGC
    AAGAAGCCTG ACCCCAAGTG TGTCTTCACC TATGTGCAGT CGCTCTACAA CCACCTGCGA
    CGCCACGAAC TGCGCCTGCG CGGCAAGAAT GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884403.1
    CDS163..3078
    Translation

    Target ORF information:

    RefSeq Version XM_017028914.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGATGGAA
    GCAGAGCCAG CAGAGCCTCT CGCTGCAGCA GTGGAAGCGG CCAATGGGGC TGAGCAGACC
    CGAGTGAACA AAGCACCAGA AGGGCGGAGC CCTCTGAGCG CTGAGGAGCT GATGACTATT
    GAGGATGAAG GAGTCTTGGA CAAGATGCTG GATCAGAGCA CGGACTTTGA AGAGCGGAAG
    CTCATCCGGG CTGCACTTCG TGAGCTCCGA CAAAGGAAGA GAGACCAGCG GGACAAGGAG
    CGGGAACGGC GGCTGCAGGA GGCACGGGGC CGGCCAGGGG AGGGGCGCGG CAACACAGCC
    ACTGAGACCA CCACGAGGCA CAGCCAGCGG GCAGCTGATG GCTCTGCTGT CAGCACTGTT
    ACCAAGACTG AGCGGCTCGT CCACTCCAAT GATGGCACAC GGACGGCCCG CACCACCACA
    GTGGAGTCGA GTTTCGTGAG GCGCTCGGAG AATGGCAGTG GCAGCACCAT GATGCAAACC
    AAGACCTTCT CCTCTTCCTC CTCATCCAAG AAGATGGGCA GCATCTTCGA CCGCGAGGAC
    CAGGCCAGCC CACGGGCCGG CAGCCTGGCG GCGCTCGAGA AACGGCAGGC CGAGAAGAAG
    AAAGAGCTGA TGAAGGCGCA GAGTCTGCCC AAGACCTCAG CCTCCCAGGC GCGCAAGGCC
    ATGATTGAGA AGCTGGAGAA GGAGGGCGCG GCCGGCAGCC CTGGCGGACC CCGCGCAGCC
    GTGCAGCGAT CCACCAGCTT CGGGGTCCCC AACGCCAACA GCATCAAGCA GATGCTGCTG
    GACTGGTGTC GAGCCAAGAC TCGCGGCTAC GAGCACGTCG ACATCCAGAA CTTCTCCTCC
    AGCTGGAGTG ATGGGATGGC CTTCTGTGCC CTGGTGCACA ACTTCTTCCC TGAGGCCTTC
    GACTATGGGC AGCTTAGCCC TCAGAACCGA CGCCAGAACT TCGAGGTGGC CTTCTCATCT
    GCGGAGATGC TGGTGGACTG TGTGCCCCTG GTGGAGGTGG ACGACATGAT GATCATGGGC
    AAGAAGCCTG ACCCCAAGTG TGTCTTCACC TATGTGCAGT CGCTCTACAA CCACCTGCGA
    CGCCACGAAC TGCGCCTGCG CGGCAAGAAT GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60556
    Clone ID Related Accession (Same CDS sequence) XM_017028915.1
    Accession Version XM_017028915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGATGGAA
    GCAGAGCCAG CAGAGCCTCT CGCTGCAGCA GTGGAAGCGG CCAATGGGGC TGAGCAGACC
    CGAGTGAACA AAGCACCAGA AGGGCGGAGC CCTCTGAGCG CTGAGGAGCT GATGACTATT
    GAGGATGAAG GAGTCTTGGA CAAGATGCTG GATCAGAGCA CGGACTTTGA AGAGCGGAAG
    CTCATCCGGG CTGCACTTCG TGAGCTCCGA CAAAGGAAGA GAGACCAGCG GGACAAGGAG
    CGGGAACGGC GGCTGCAGGA GGCACGGGGC CGGCCAGGGG AGGGGCGCGG CAACACAGCC
    ACTGAGACCA CCACGAGGCA CAGCCAGCGG GCAGCTGATG GCTCTGCTGT CAGCACTGTT
    ACCAAGACTG AGCGGCTCGT CCACTCCAAT GATGGCACAC GGACGGCCCG CACCACCACA
    GTGGAGTCGA GTTTCGTGAG GCGCTCGGAG AATGGCAGTG GCAGCACCAT GATGCAAACC
    AAGACCTTCT CCTCTTCCTC CTCATCCAAG AAGATGGGCA GCATCTTCGA CCGCGAGGAC
    CAGGCCAGCC CACGGGCCGG CAGCCTGGCG GCGCTCGAGA AACGGCAGGC CGAGAAGAAG
    AAAGAGCTGA TGAAGGCGCA GAGTCTGCCC AAGACCTCAG CCTCCCAGGC GCGCAAGGCC
    ATGATTGAGA AGCTGGAGAA GGAGGGCGCG GCCGGCAGCC CTGGCGGACC CCGCGCAGCC
    GTGCAGCGAT CCACCAGCTT CGGGGTCCCC AACGCCAACA GCATCAAGCA GATGCTGCTG
    GACTGGTGTC GAGCCAAGAC TCGCGGCTAC GAGCACGTCG ACATCCAGAA CTTCTCCTCC
    AGCTGGAGTG ATGGGATGGC CTTCTGTGCC CTGGTGCACA ACTTCTTCCC TGAGGCCTTC
    GACTATGGGC AGCTTAGCCC TCAGAACCGA CGCCAGAACT TCGAGGTGGC CTTCTCATCT
    GCGGAGACCC ATGCGGACTG CCCGCAGCTC CTGGATACAG AGGACATGGT GCGGCTTCGA
    GAGCCTGACT GGAAGTGCGT GTACACGTAC ATCCAGGAAT TCTACCGCTG TCTGGTCCAG
    AAGGGGCTGG TAAAAACCAA AAAGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884404.1
    CDS163..3072
    Translation

    Target ORF information:

    RefSeq Version XM_017028915.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGATGGAA
    GCAGAGCCAG CAGAGCCTCT CGCTGCAGCA GTGGAAGCGG CCAATGGGGC TGAGCAGACC
    CGAGTGAACA AAGCACCAGA AGGGCGGAGC CCTCTGAGCG CTGAGGAGCT GATGACTATT
    GAGGATGAAG GAGTCTTGGA CAAGATGCTG GATCAGAGCA CGGACTTTGA AGAGCGGAAG
    CTCATCCGGG CTGCACTTCG TGAGCTCCGA CAAAGGAAGA GAGACCAGCG GGACAAGGAG
    CGGGAACGGC GGCTGCAGGA GGCACGGGGC CGGCCAGGGG AGGGGCGCGG CAACACAGCC
    ACTGAGACCA CCACGAGGCA CAGCCAGCGG GCAGCTGATG GCTCTGCTGT CAGCACTGTT
    ACCAAGACTG AGCGGCTCGT CCACTCCAAT GATGGCACAC GGACGGCCCG CACCACCACA
    GTGGAGTCGA GTTTCGTGAG GCGCTCGGAG AATGGCAGTG GCAGCACCAT GATGCAAACC
    AAGACCTTCT CCTCTTCCTC CTCATCCAAG AAGATGGGCA GCATCTTCGA CCGCGAGGAC
    CAGGCCAGCC CACGGGCCGG CAGCCTGGCG GCGCTCGAGA AACGGCAGGC CGAGAAGAAG
    AAAGAGCTGA TGAAGGCGCA GAGTCTGCCC AAGACCTCAG CCTCCCAGGC GCGCAAGGCC
    ATGATTGAGA AGCTGGAGAA GGAGGGCGCG GCCGGCAGCC CTGGCGGACC CCGCGCAGCC
    GTGCAGCGAT CCACCAGCTT CGGGGTCCCC AACGCCAACA GCATCAAGCA GATGCTGCTG
    GACTGGTGTC GAGCCAAGAC TCGCGGCTAC GAGCACGTCG ACATCCAGAA CTTCTCCTCC
    AGCTGGAGTG ATGGGATGGC CTTCTGTGCC CTGGTGCACA ACTTCTTCCC TGAGGCCTTC
    GACTATGGGC AGCTTAGCCC TCAGAACCGA CGCCAGAACT TCGAGGTGGC CTTCTCATCT
    GCGGAGACCC ATGCGGACTG CCCGCAGCTC CTGGATACAG AGGACATGGT GCGGCTTCGA
    GAGCCTGACT GGAAGTGCGT GTACACGTAC ATCCAGGAAT TCTACCGCTG TCTGGTCCAG
    AAGGGGCTGG TAAAAACCAA AAAGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60557
    Clone ID Related Accession (Same CDS sequence) XM_011530336.2
    Accession Version XM_011530336.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530336.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGATGG CAGTGGCAGC ACCATGATGC AAACCAAGAC CTTCTCCTCT
    TCCTCCTCAT CCAAGAAGAT GGGCAGCATC TTCGACCGCG AGGACCAGGC CAGCCCACGG
    GCCGGCAGCC TGGCGGCGCT CGAGAAACGG CAGGCCGAGA AGAAGAAAGA GCTGATGAAG
    GCGCAGAGTC TGCCCAAGAC CTCAGCCTCC CAGGCGCGCA AGGCCATGAT TGAGAAGCTG
    GAGAAGGAGG GCGCGGCCGG CAGCCCTGGC GGACCCCGCG CAGCCGTGCA GCGATCCACC
    AGCTTCGGGG TCCCCAACGC CAACAGCATC AAGCAGATGC TGCTGGACTG GTGTCGAGCC
    AAGACTCGCG GCTACGAGCA CGTCGACATC CAGAACTTCT CCTCCAGCTG GAGTGATGGG
    ATGGCCTTCT GTGCCCTGGT GCACAACTTC TTCCCTGAGG CCTTCGACTA TGGGCAGCTT
    AGCCCTCAGA ACCGACGCCA GAACTTCGAG GTGGCCTTCT CATCTGCGGA GATGCTGGTG
    GACTGTGTGC CCCTGGTGGA GGTGGACGAC ATGATGATCA TGGGCAAGAA GCCTGACCCC
    AAGTGTGTCT TCACCTATGT GCAGTCGCTC TACAACCACC TGCGACGCCA CGAACTGCGC
    CTGCGCGGCA AGAATGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528638.1
    CDS164..2944
    Translation

    Target ORF information:

    RefSeq Version XM_011530336.2
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530336.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGATGG CAGTGGCAGC ACCATGATGC AAACCAAGAC CTTCTCCTCT
    TCCTCCTCAT CCAAGAAGAT GGGCAGCATC TTCGACCGCG AGGACCAGGC CAGCCCACGG
    GCCGGCAGCC TGGCGGCGCT CGAGAAACGG CAGGCCGAGA AGAAGAAAGA GCTGATGAAG
    GCGCAGAGTC TGCCCAAGAC CTCAGCCTCC CAGGCGCGCA AGGCCATGAT TGAGAAGCTG
    GAGAAGGAGG GCGCGGCCGG CAGCCCTGGC GGACCCCGCG CAGCCGTGCA GCGATCCACC
    AGCTTCGGGG TCCCCAACGC CAACAGCATC AAGCAGATGC TGCTGGACTG GTGTCGAGCC
    AAGACTCGCG GCTACGAGCA CGTCGACATC CAGAACTTCT CCTCCAGCTG GAGTGATGGG
    ATGGCCTTCT GTGCCCTGGT GCACAACTTC TTCCCTGAGG CCTTCGACTA TGGGCAGCTT
    AGCCCTCAGA ACCGACGCCA GAACTTCGAG GTGGCCTTCT CATCTGCGGA GATGCTGGTG
    GACTGTGTGC CCCTGGTGGA GGTGGACGAC ATGATGATCA TGGGCAAGAA GCCTGACCCC
    AAGTGTGTCT TCACCTATGT GCAGTCGCTC TACAACCACC TGCGACGCCA CGAACTGCGC
    CTGCGCGGCA AGAATGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60558
    Clone ID Related Accession (Same CDS sequence) XM_011530337.2
    Accession Version XM_011530337.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530337.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGATGG CAGTGGCAGC ACCATGATGC AAACCAAGAC CTTCTCCTCT
    TCCTCCTCAT CCAAGAAGAT GGGCAGCATC TTCGACCGCG AGGACCAGGC CAGCCCACGG
    GCCGGCAGCC TGGCGGCGCT CGAGAAACGG CAGGCCGAGA AGAAGAAAGA GCTGATGAAG
    GCGCAGAGTC TGCCCAAGAC CTCAGCCTCC CAGGCGCGCA AGGCCATGAT TGAGAAGCTG
    GAGAAGGAGG GCGCGGCCGG CAGCCCTGGC GGACCCCGCG CAGCCGTGCA GCGATCCACC
    AGCTTCGGGG TCCCCAACGC CAACAGCATC AAGCAGATGC TGCTGGACTG GTGTCGAGCC
    AAGACTCGCG GCTACGAGCA CGTCGACATC CAGAACTTCT CCTCCAGCTG GAGTGATGGG
    ATGGCCTTCT GTGCCCTGGT GCACAACTTC TTCCCTGAGG CCTTCGACTA TGGGCAGCTT
    AGCCCTCAGA ACCGACGCCA GAACTTCGAG GTGGCCTTCT CATCTGCGGA GACCCATGCG
    GACTGCCCGC AGCTCCTGGA TACAGAGGAC ATGGTGCGGC TTCGAGAGCC TGACTGGAAG
    TGCGTGTACA CGTACATCCA GGAATTCTAC CGCTGTCTGG TCCAGAAGGG GCTGGTAAAA
    ACCAAAAAGT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528639.1
    CDS164..2938
    Translation

    Target ORF information:

    RefSeq Version XM_011530337.2
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530337.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGATGG CAGTGGCAGC ACCATGATGC AAACCAAGAC CTTCTCCTCT
    TCCTCCTCAT CCAAGAAGAT GGGCAGCATC TTCGACCGCG AGGACCAGGC CAGCCCACGG
    GCCGGCAGCC TGGCGGCGCT CGAGAAACGG CAGGCCGAGA AGAAGAAAGA GCTGATGAAG
    GCGCAGAGTC TGCCCAAGAC CTCAGCCTCC CAGGCGCGCA AGGCCATGAT TGAGAAGCTG
    GAGAAGGAGG GCGCGGCCGG CAGCCCTGGC GGACCCCGCG CAGCCGTGCA GCGATCCACC
    AGCTTCGGGG TCCCCAACGC CAACAGCATC AAGCAGATGC TGCTGGACTG GTGTCGAGCC
    AAGACTCGCG GCTACGAGCA CGTCGACATC CAGAACTTCT CCTCCAGCTG GAGTGATGGG
    ATGGCCTTCT GTGCCCTGGT GCACAACTTC TTCCCTGAGG CCTTCGACTA TGGGCAGCTT
    AGCCCTCAGA ACCGACGCCA GAACTTCGAG GTGGCCTTCT CATCTGCGGA GACCCATGCG
    GACTGCCCGC AGCTCCTGGA TACAGAGGAC ATGGTGCGGC TTCGAGAGCC TGACTGGAAG
    TGCGTGTACA CGTACATCCA GGAATTCTAC CGCTGTCTGG TCCAGAAGGG GCTGGTAAAA
    ACCAAAAAGT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60559
    Clone ID Related Accession (Same CDS sequence) XM_011530338.2
    Accession Version XM_011530338.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGATGGAA
    GCAGAGCCAG CAGAGCCTCT CGCTGCAGCA GTGGAAGCGG CCAATGGGGC TGAGCAGACC
    CGAGTGAACA AAGCACCAGA AGGGCGGAGC CCTCTGAGCG CTGAGGAGCT GATGACTATT
    GAGGATGAAG GAGTCTTGGA CAAGATGCTG GATCAGAGCA CGGACTTTGA AGAGCGGAAG
    CTCATCCGGG CTGCACTTCG TGAGCTCCGA CAAAGGAAGA GAGATGGCAG TGGCAGCACC
    ATGATGCAAA CCAAGACCTT CTCCTCTTCC TCCTCATCCA AGAAGATGGG CAGCATCTTC
    GACCGCGAGG ACCAGGCCAG CCCACGGGCC GGCAGCCTGG CGGCGCTCGA GAAACGGCAG
    GCCGAGAAGA AGAAAGAGCT GATGAAGGCG CAGAGTCTGC CCAAGACCTC AGCCTCCCAG
    GCGCGCAAGG CCATGATTGA GAAGCTGGAG AAGGAGGGCG CGGCCGGCAG CCCTGGCGGA
    CCCCGCGCAG CCGTGCAGCG ATCCACCAGC TTCGGGGTCC CCAACGCCAA CAGCATCAAG
    CAGATGCTGC TGGACTGGTG TCGAGCCAAG ACTCGCGGCT ACGAGCACGT CGACATCCAG
    AACTTCTCCT CCAGCTGGAG TGATGGGATG GCCTTCTGTG CCCTGGTGCA CAACTTCTTC
    CCTGAGGCCT TCGACTATGG GCAGCTTAGC CCTCAGAACC GACGCCAGAA CTTCGAGGTG
    GCCTTCTCAT CTGCGGAGAT GCTGGTGGAC TGTGTGCCCC TGGTGGAGGT GGACGACATG
    ATGATCATGG GCAAGAAGCC TGACCCCAAG TGTGTCTTCA CCTATGTGCA GTCGCTCTAC
    AACCACCTGC GACGCCACGA ACTGCGCCTG CGCGGCAAGA ATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528640.1
    CDS163..2850
    Translation

    Target ORF information:

    RefSeq Version XM_011530338.2
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530338.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGATGGAA
    GCAGAGCCAG CAGAGCCTCT CGCTGCAGCA GTGGAAGCGG CCAATGGGGC TGAGCAGACC
    CGAGTGAACA AAGCACCAGA AGGGCGGAGC CCTCTGAGCG CTGAGGAGCT GATGACTATT
    GAGGATGAAG GAGTCTTGGA CAAGATGCTG GATCAGAGCA CGGACTTTGA AGAGCGGAAG
    CTCATCCGGG CTGCACTTCG TGAGCTCCGA CAAAGGAAGA GAGATGGCAG TGGCAGCACC
    ATGATGCAAA CCAAGACCTT CTCCTCTTCC TCCTCATCCA AGAAGATGGG CAGCATCTTC
    GACCGCGAGG ACCAGGCCAG CCCACGGGCC GGCAGCCTGG CGGCGCTCGA GAAACGGCAG
    GCCGAGAAGA AGAAAGAGCT GATGAAGGCG CAGAGTCTGC CCAAGACCTC AGCCTCCCAG
    GCGCGCAAGG CCATGATTGA GAAGCTGGAG AAGGAGGGCG CGGCCGGCAG CCCTGGCGGA
    CCCCGCGCAG CCGTGCAGCG ATCCACCAGC TTCGGGGTCC CCAACGCCAA CAGCATCAAG
    CAGATGCTGC TGGACTGGTG TCGAGCCAAG ACTCGCGGCT ACGAGCACGT CGACATCCAG
    AACTTCTCCT CCAGCTGGAG TGATGGGATG GCCTTCTGTG CCCTGGTGCA CAACTTCTTC
    CCTGAGGCCT TCGACTATGG GCAGCTTAGC CCTCAGAACC GACGCCAGAA CTTCGAGGTG
    GCCTTCTCAT CTGCGGAGAT GCTGGTGGAC TGTGTGCCCC TGGTGGAGGT GGACGACATG
    ATGATCATGG GCAAGAAGCC TGACCCCAAG TGTGTCTTCA CCTATGTGCA GTCGCTCTAC
    AACCACCTGC GACGCCACGA ACTGCGCCTG CGCGGCAAGA ATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60560
    Clone ID Related Accession (Same CDS sequence) XM_011530339.2
    Accession Version XM_011530339.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530339.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCC AGATACTCCC TCCCGCAGCT ACTCTCTCCT TGGATCCAGT
    TGCCTCTCAA AGTACTGTCA ACGACTCCTT CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528641.1
    CDS164..2359
    Translation

    Target ORF information:

    RefSeq Version XM_011530339.2
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530339.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCC AGATACTCCC TCCCGCAGCT ACTCTCTCCT TGGATCCAGT
    TGCCTCTCAA AGTACTGTCA ACGACTCCTT CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60561
    Clone ID Related Accession (Same CDS sequence) XM_011530340.2
    Accession Version XM_011530340.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2103bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530340.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGATGGAA
    GCAGAGCCAG CAGAGCCTCT CGCTGCAGCA GTGGAAGCGG CCAATGGGGC TGAGCAGACC
    CGAGTGAACA AAGCACCAGA AGGGCGGAGC CCTCTGAGCG CTGAGGAGCT GATGACTATT
    GAGGATGAAG GAGTCTTGGA CAAGATGCTG GATCAGAGCA CGGACTTTGA AGAGCGGAAG
    CTCATCCGGG CTGCACTTCG TGAGCTCCGA CAAAGGAAGA GAGACCCAGA TACTCCCTCC
    CGCAGCTACT CTCTCCTTGG ATCCAGTTGC CTCTCAAAGT ACTGTCAACG ACTCCTTCCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528642.1
    CDS164..2266
    Translation

    Target ORF information:

    RefSeq Version XM_011530340.2
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530340.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGATGGAA
    GCAGAGCCAG CAGAGCCTCT CGCTGCAGCA GTGGAAGCGG CCAATGGGGC TGAGCAGACC
    CGAGTGAACA AAGCACCAGA AGGGCGGAGC CCTCTGAGCG CTGAGGAGCT GATGACTATT
    GAGGATGAAG GAGTCTTGGA CAAGATGCTG GATCAGAGCA CGGACTTTGA AGAGCGGAAG
    CTCATCCGGG CTGCACTTCG TGAGCTCCGA CAAAGGAAGA GAGACCCAGA TACTCCCTCC
    CGCAGCTACT CTCTCCTTGG ATCCAGTTGC CTCTCAAAGT ACTGTCAACG ACTCCTTCCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93564
    Clone ID Related Accession (Same CDS sequence) XM_017028916.1
    Accession Version XM_017028916.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA CGTCGACATC CAGAACTTCT CCTCCAGCTG GAGTGATGGG ATGGCCTTCT
    GTGCCCTGGT GCACAACTTC TTCCCTGAGG CCTTCGACTA TGGGCAGCTT AGCCCTCAGA
    ACCGACGCCA GAACTTCGAG GTGGCCTTCT CATCTGCGGA GACCCATGCG GACTGCCCGC
    AGCTCCTGGA TACAGAGGAC ATGGTGCGGC TTCGAGAGCC TGACTGGAAG TGCGTGTACA
    CGTACATCCA GGAATTCTAC CGCTGTCTGG TCCAGAAGGG GCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884405.1
    CDS164..3031
    Translation

    Target ORF information:

    RefSeq Version XM_017028916.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028916.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA CGTCGACATC CAGAACTTCT CCTCCAGCTG GAGTGATGGG ATGGCCTTCT
    GTGCCCTGGT GCACAACTTC TTCCCTGAGG CCTTCGACTA TGGGCAGCTT AGCCCTCAGA
    ACCGACGCCA GAACTTCGAG GTGGCCTTCT CATCTGCGGA GACCCATGCG GACTGCCCGC
    AGCTCCTGGA TACAGAGGAC ATGGTGCGGC TTCGAGAGCC TGACTGGAAG TGCGTGTACA
    CGTACATCCA GGAATTCTAC CGCTGTCTGG TCCAGAAGGG GCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93565
    Clone ID Related Accession (Same CDS sequence) XM_017028917.1
    Accession Version XM_017028917.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA CGTCGACATC CAGAACTTCT CCTCCAGCTG GAGTGATGGG ATGGCCTTCT
    GTGCCCTGGT GCACAACTTC TTCCCTGAGG CCTTCGACTA TGGGCAGCTT AGCCCTCAGA
    ACCGACGCCA GAACTTCGAG GTGGCCTTCT CATCTGCGGA GATGCTGGTG GACTGTGTGC
    CCCTGGTGGA GGTGGACGAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884406.1
    CDS164..2947
    Translation

    Target ORF information:

    RefSeq Version XM_017028917.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028917.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA CGTCGACATC CAGAACTTCT CCTCCAGCTG GAGTGATGGG ATGGCCTTCT
    GTGCCCTGGT GCACAACTTC TTCCCTGAGG CCTTCGACTA TGGGCAGCTT AGCCCTCAGA
    ACCGACGCCA GAACTTCGAG GTGGCCTTCT CATCTGCGGA GATGCTGGTG GACTGTGTGC
    CCCTGGTGGA GGTGGACGAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93566
    Clone ID Related Accession (Same CDS sequence) XM_017028918.1
    Accession Version XM_017028918.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAGTAAGG CCACCTGGTG TCGCCCTGTG CCTGCCTGCC TGTCCGCCCA CCTCCTCCGG
    CTCTTCCTTG AGCTCTCTCT CCGTGTCTTC AACTGTGCCG TCACTTTCTC TTCTCTGCCT
    GCGGCTATCA CCACCCCTCC ATACAGCCTC CCATCCCATC TCCTGATGTC CAGCCCAGGC
    CCTCACCTGG TGCCCCTTCC CCTTTTTTGC AGATGGCAGT GGCAGCACCA TGATGCAAAC
    CAAGACCTTC TCCTCTTCCT CCTCATCCAA GAAGATGGGC AGCATCTTCG ACCGCGAGGA
    CCAGGCCAGC CCACGGGCCG GCAGCCTGGC GGCGCTCGAG AAACGGCAGG CCGAGAAGAA
    GAAAGAGCTG ATGAAGGCGC AGAGTCTGCC CAAGACCTCA GCCTCCCAGG CGCGCAAGGC
    CATGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884407.1
    CDS164..2932
    Translation

    Target ORF information:

    RefSeq Version XM_017028918.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAGTAAGG CCACCTGGTG TCGCCCTGTG CCTGCCTGCC TGTCCGCCCA CCTCCTCCGG
    CTCTTCCTTG AGCTCTCTCT CCGTGTCTTC AACTGTGCCG TCACTTTCTC TTCTCTGCCT
    GCGGCTATCA CCACCCCTCC ATACAGCCTC CCATCCCATC TCCTGATGTC CAGCCCAGGC
    CCTCACCTGG TGCCCCTTCC CCTTTTTTGC AGATGGCAGT GGCAGCACCA TGATGCAAAC
    CAAGACCTTC TCCTCTTCCT CCTCATCCAA GAAGATGGGC AGCATCTTCG ACCGCGAGGA
    CCAGGCCAGC CCACGGGCCG GCAGCCTGGC GGCGCTCGAG AAACGGCAGG CCGAGAAGAA
    GAAAGAGCTG ATGAAGGCGC AGAGTCTGCC CAAGACCTCA GCCTCCCAGG CGCGCAAGGC
    CATGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93567
    Clone ID Related Accession (Same CDS sequence) XM_017028919.1
    Accession Version XM_017028919.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGATGGAA
    GCAGAGCCAG CAGAGCCTCT CGCTGCAGCA GTGGAAGCGG CCAATGGGGC TGAGCAGACC
    CGAGTGAACA AAGCACCAGA AGGGCGGAGC CCTCTGAGCG CTGAGGAGCT GATGACTATT
    GAGGATGAAG GAGTCTTGGA CAAGATGCTG GATCAGAGCA CGGACTTTGA AGAGCGGAAG
    CTCATCCGGG CTGCACTTCG TGAGCTCCGA CAAAGGAAGA GAGATGGCAG TGGCAGCACC
    ATGATGCAAA CCAAGACCTT CTCCTCTTCC TCCTCATCCA AGAAGATGGG CAGCATCTTC
    GACCGCGAGG ACCAGGCCAG CCCACGGGCC GGCAGCCTGG CGGCGCTCGA GAAACGGCAG
    GCCGAGAAGA AGAAAGAGCT GATGAAGGCG CAGAGTCTGC CCAAGACCTC AGCCTCCCAG
    GCGCGCAAGG CCATGATTGA GAAGCTGGAG AAGGAGGGCG CGGCCGGCAG CCCTGGCGGA
    CCCCGCGCAG CCGTGCAGCG ATCCACCAGC TTCGGGGTCC CCAACGCCAA CAGCATCAAG
    CAGATGCTGC TGGACTGGTG TCGAGCCAAG ACTCGCGGCT ACGAGCACGT CGACATCCAG
    AACTTCTCCT CCAGCTGGAG TGATGGGATG GCCTTCTGTG CCCTGGTGCA CAACTTCTTC
    CCTGAGGCCT TCGACTATGG GCAGCTTAGC CCTCAGAACC GACGCCAGAA CTTCGAGGTG
    GCCTTCTCAT CTGCGGAGAC CCATGCGGAC TGCCCGCAGC TCCTGGATAC AGAGGACATG
    GTGCGGCTTC GAGAGCCTGA CTGGAAGTGC GTGTACACGT ACATCCAGGA ATTCTACCGC
    TGTCTGGTCC AGAAGGGGCT GGTAAAAACC AAAAAGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884408.1
    CDS163..2844
    Translation

    Target ORF information:

    RefSeq Version XM_017028919.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028919.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGATGGAA
    GCAGAGCCAG CAGAGCCTCT CGCTGCAGCA GTGGAAGCGG CCAATGGGGC TGAGCAGACC
    CGAGTGAACA AAGCACCAGA AGGGCGGAGC CCTCTGAGCG CTGAGGAGCT GATGACTATT
    GAGGATGAAG GAGTCTTGGA CAAGATGCTG GATCAGAGCA CGGACTTTGA AGAGCGGAAG
    CTCATCCGGG CTGCACTTCG TGAGCTCCGA CAAAGGAAGA GAGATGGCAG TGGCAGCACC
    ATGATGCAAA CCAAGACCTT CTCCTCTTCC TCCTCATCCA AGAAGATGGG CAGCATCTTC
    GACCGCGAGG ACCAGGCCAG CCCACGGGCC GGCAGCCTGG CGGCGCTCGA GAAACGGCAG
    GCCGAGAAGA AGAAAGAGCT GATGAAGGCG CAGAGTCTGC CCAAGACCTC AGCCTCCCAG
    GCGCGCAAGG CCATGATTGA GAAGCTGGAG AAGGAGGGCG CGGCCGGCAG CCCTGGCGGA
    CCCCGCGCAG CCGTGCAGCG ATCCACCAGC TTCGGGGTCC CCAACGCCAA CAGCATCAAG
    CAGATGCTGC TGGACTGGTG TCGAGCCAAG ACTCGCGGCT ACGAGCACGT CGACATCCAG
    AACTTCTCCT CCAGCTGGAG TGATGGGATG GCCTTCTGTG CCCTGGTGCA CAACTTCTTC
    CCTGAGGCCT TCGACTATGG GCAGCTTAGC CCTCAGAACC GACGCCAGAA CTTCGAGGTG
    GCCTTCTCAT CTGCGGAGAC CCATGCGGAC TGCCCGCAGC TCCTGGATAC AGAGGACATG
    GTGCGGCTTC GAGAGCCTGA CTGGAAGTGC GTGTACACGT ACATCCAGGA ATTCTACCGC
    TGTCTGGTCC AGAAGGGGCT GGTAAAAACC AAAAAGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14968
    Clone ID Related Accession (Same CDS sequence) XM_017028913.1 , NM_001207017.1
    Accession Version XM_017028913.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA GCCCTGGCGG ACCCCGCGCA GCCGTGCAGC GATCCACCAG CTTCGGGGTC
    CCCAACGCCA ACAGCATCAA GCAGATGCTG CTGGACTGGT GTCGAGCCAA GACTCGCGGC
    TACGAGCACG TCGACATCCA GAACTTCTCC TCCAGCTGGA GTGATGGGAT GGCCTTCTGT
    GCCCTGGTGC ACAACTTCTT CCCTGAGGCC TTCGACTATG GGCAGCTTAG CCCTCAGAAC
    CGACGCCAGA ACTTCGAGGT GGCCTTCTCA TCTGCGGAGA TGCTGGTGGA CTGTGTGCCC
    CTGGTGGAGG TGGACGACAT GATGATCATG GGCAAGAAGC CTGACCCCAA GTGTGTCTTC
    ACCTATGTGC AGTCGCTCTA CAACCACCTG CGACGCCACG AACTGCGCCT GCGCGGCAAG
    AATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884402.1
    CDS164..3172
    Translation

    Target ORF information:

    RefSeq Version XM_017028913.1
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028913.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA GCCCTGGCGG ACCCCGCGCA GCCGTGCAGC GATCCACCAG CTTCGGGGTC
    CCCAACGCCA ACAGCATCAA GCAGATGCTG CTGGACTGGT GTCGAGCCAA GACTCGCGGC
    TACGAGCACG TCGACATCCA GAACTTCTCC TCCAGCTGGA GTGATGGGAT GGCCTTCTGT
    GCCCTGGTGC ACAACTTCTT CCCTGAGGCC TTCGACTATG GGCAGCTTAG CCCTCAGAAC
    CGACGCCAGA ACTTCGAGGT GGCCTTCTCA TCTGCGGAGA TGCTGGTGGA CTGTGTGCCC
    CTGGTGGAGG TGGACGACAT GATGATCATG GGCAAGAAGC CTGACCCCAA GTGTGTCTTC
    ACCTATGTGC AGTCGCTCTA CAACCACCTG CGACGCCACG AACTGCGCCT GCGCGGCAAG
    AATGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60554
    Clone ID Related Accession (Same CDS sequence) XM_011530333.2 , XM_011530333.3
    Accession Version XM_011530333.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3003bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA GCCCTGGCGG ACCCCGCGCA GCCGTGCAGC GATCCACCAG CTTCGGGGTC
    CCCAACGCCA ACAGCATCAA GCAGATGCTG CTGGACTGGT GTCGAGCCAA GACTCGCGGC
    TACGAGCACG TCGACATCCA GAACTTCTCC TCCAGCTGGA GTGATGGGAT GGCCTTCTGT
    GCCCTGGTGC ACAACTTCTT CCCTGAGGCC TTCGACTATG GGCAGCTTAG CCCTCAGAAC
    CGACGCCAGA ACTTCGAGGT GGCCTTCTCA TCTGCGGAGA CCCATGCGGA CTGCCCGCAG
    CTCCTGGATA CAGAGGACAT GGTGCGGCTT CGAGAGCCTG ACTGGAAGTG CGTGTACACG
    TACATCCAGG AATTCTACCG CTGTCTGGTC CAGAAGGGGC TGGTAAAAAC CAAAAAGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528635.1
    CDS163..3165
    Translation

    Target ORF information:

    RefSeq Version XM_011530333.2
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530333.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA GCCCTGGCGG ACCCCGCGCA GCCGTGCAGC GATCCACCAG CTTCGGGGTC
    CCCAACGCCA ACAGCATCAA GCAGATGCTG CTGGACTGGT GTCGAGCCAA GACTCGCGGC
    TACGAGCACG TCGACATCCA GAACTTCTCC TCCAGCTGGA GTGATGGGAT GGCCTTCTGT
    GCCCTGGTGC ACAACTTCTT CCCTGAGGCC TTCGACTATG GGCAGCTTAG CCCTCAGAAC
    CGACGCCAGA ACTTCGAGGT GGCCTTCTCA TCTGCGGAGA CCCATGCGGA CTGCCCGCAG
    CTCCTGGATA CAGAGGACAT GGTGCGGCTT CGAGAGCCTG ACTGGAAGTG CGTGTACACG
    TACATCCAGG AATTCTACCG CTGTCTGGTC CAGAAGGGGC TGGTAAAAAC CAAAAAGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60554
    Clone ID Related Accession (Same CDS sequence) XM_011530333.2 , XM_011530333.3
    Accession Version XM_011530333.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3003bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product smoothelin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530333.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA GCCCTGGCGG ACCCCGCGCA GCCGTGCAGC GATCCACCAG CTTCGGGGTC
    CCCAACGCCA ACAGCATCAA GCAGATGCTG CTGGACTGGT GTCGAGCCAA GACTCGCGGC
    TACGAGCACG TCGACATCCA GAACTTCTCC TCCAGCTGGA GTGATGGGAT GGCCTTCTGT
    GCCCTGGTGC ACAACTTCTT CCCTGAGGCC TTCGACTATG GGCAGCTTAG CCCTCAGAAC
    CGACGCCAGA ACTTCGAGGT GGCCTTCTCA TCTGCGGAGA CCCATGCGGA CTGCCCGCAG
    CTCCTGGATA CAGAGGACAT GGTGCGGCTT CGAGAGCCTG ACTGGAAGTG CGTGTACACG
    TACATCCAGG AATTCTACCG CTGTCTGGTC CAGAAGGGGC TGGTAAAAAC CAAAAAGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528635.1
    CDS164..3166
    Translation

    Target ORF information:

    RefSeq Version XM_011530333.3
    Organism Homo sapiens(human)
    Definition Homo sapiens smoothelin (SMTN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530333.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGAGTCC ATCCCAGGCC ACACAGCAGA CTGGCGGCCA AGCTGGCAGG GCTGGAACCA 
    GAGACCCCCT ACCCTGGGTT TGAATTCTCT GAGCTGGTGA CAGGTGCCAC AGGCACTGGG
    GATCTCACCA GAAAGGAACC GACGGAGCTA GGGGCCAGCG AGATGGCGGA CGAGGCCTTA
    GCTGGGCTGG ATGAGGGAGC CCTTCGGAAG CTGCTGGAGG TCACAGCAGA TCTGGCAGAG
    CGGCGGCGCA TCCGCTCAGC CATCCGGGAA CTGCAGCGGC AGGAGCTGGA GCGCGAGGAG
    GAGGCCCTGG CATCCAAGCG TTTCCGTGCC GAGCGGCAGG ACAACAAGGA GAACTGGCTG
    CACTCTCAGC AGCGGGAAGC TGAGCAGCGG GCTGCCCTGG CACGGCTGGC AGGGCAGCTG
    GAGTCCATGA ACGATGTGGA GGAATTGACT GCACTGTTGC GAAGCGCTGG TGAGTATGAG
    GAGCGCAAGC TGATCCGAGC TGCCATCCGC CGTGTACGGG CTCAGGAGAT TGAGGCTGCC
    ACCTTGGCTG GGAGGTTGTA CAGCGGGCGT CCCAACAGTG GCTCAAGAGA GGACAGCAAG
    GGGCTAGCGG CACACAGGCT GGAACAGTGT GAGGTGCCAG AGCGAGAGGA ACAGGAACAG
    CAGGCAGAGG TTTCAAAGCC AACCCCCACC CCTGAAGGCA CCAGCCAGGA TGTGACCACA
    GTGACACTCC TGCTGCGAGC CCCACCTGGG AGCACATCCA GCTCACCTGC CTCACCCAGC
    AGTTCACCCA CCCCTGCCTC TCCTGAGCCT CCATTGGAGC CTGCCGAGGC CCAGTGCCTT
    ACAGCTGAGG TTCCAGGCAG CCCAGAGCCA CCCCCCAGCC CACCCAAGAC CACCAGCCCT
    GAGCCTCAGG AGTCTCCAAC GCTCCCCAGC ACTGAGGGCC AGGTGGTCAA CAAGCTTCTG
    TCTGGCCCCA AAGAGACCCC TGCTGCCCAG AGCCCCACCA GAGGCCCCTC TGACACCAAG
    AGAGCAGACG TGGCTGGACC CCGACCCTGC CAACGCTCCC TGTCGGTGCT CAGCCCCCGC
    CAACCAGCCC AGAACCGAGA GTCCACCCCC CTTGCCAGCG GACCTTCCTC ATTCCAGCGG
    GCTGGCTCTG TGCGGGATCG TGTCCACAAG TTCACATCTG ATTCTCCTAT GGCTGCTAGG
    CTCCAGGATG GCACACCCCA GGCTGCCCTA AGTCCCCTGA CCCCCGCAAG GCTCCTGGGC
    CCCTCCCTCA CCAGCACCAC CCCTGCCTCC TCCTCCAGCG GCTCCTCCTC TCGGGGCCCC
    AGTGATACCT CCTCCCGGTT CAGCAAGGAG CAACGAGGAG TAGCCCAGCC CCTGGCCCAG
    CTTCGAAGCT GCCCCCAGGA GGAGGGCCCC AGGGGGCGGG GCTTGGCTGC TAGGCCCCTT
    GAAAACAGAG CAGGGGGGCC TGTGGCACGT TCAGAGGAGC CTGGTGCCCC GCTGCCCGTG
    GCCGTCGGCA CTGCCGAGCC AGGGGGCAGT ATGAAGACCA CATTCACCAT CGAGATCAAG
    GACGGCCGTG GCCAGGCCTC CACAGGCCGG GTGCTGCTGC CCACAGGCAA CCAGAGGGCA
    GAACTGACAC TGGGGCTGCG GGCGCCCCCG ACCCTACTCA GCACCAGTAG TGGGGGCAAG
    AGCACCATCA CCCGTGTCAA CAGCCCTGGG ACCCTGGCTC GGCTGGGCAG TGTCACTCAT
    GTCACCAGCT TCAGCCATGC CCCCCCCAGT AGCCGAGGAG GCTGCAGCAT CAAGGCTGCC
    GAGGATGCCG GGACCCCCGT GGCCCACCCA CCTGCCTTCA GCACCCGCCG CCGCTCCTCC
    ACCGGCACCA CCCGCAGCAC TAGTCTCATG GAAGCAGAGC CAGCAGAGCC TCTCGCTGCA
    GCAGTGGAAG CGGCCAATGG GGCTGAGCAG ACCCGAGTGA ACAAAGCACC AGAAGGGCGG
    AGCCCTCTGA GCGCTGAGGA GCTGATGACT ATTGAGGATG AAGGAGTCTT GGACAAGATG
    CTGGATCAGA GCACGGACTT TGAAGAGCGG AAGCTCATCC GGGCTGCACT TCGTGAGCTC
    CGACAAAGGA AGAGAGACCA GCGGGACAAG GAGCGGGAAC GGCGGCTGCA GGAGGCACGG
    GGCCGGCCAG GGGAGGGGCG CGGCAACACA GCCACTGAGA CCACCACGAG GCACAGCCAG
    CGGGCAGCTG ATGGCTCTGC TGTCAGCACT GTTACCAAGA CTGAGCGGCT CGTCCACTCC
    AATGATGGCA CACGGACGGC CCGCACCACC ACAGTGGAGT CGAGTTTCGT GAGGCGCTCG
    GAGAATGGCA GTGGCAGCAC CATGATGCAA ACCAAGACCT TCTCCTCTTC CTCCTCATCC
    AAGAAGATGG GCAGCATCTT CGACCGCGAG GACCAGGCCA GCCCACGGGC CGGCAGCCTG
    GCGGCGCTCG AGAAACGGCA GGCCGAGAAG AAGAAAGAGC TGATGAAGGC GCAGAGTCTG
    CCCAAGACCT CAGCCTCCCA GGCGCGCAAG GCCATGATTG AGAAGCTGGA GAAGGAGGGC
    GCGGCCGGCA GCCCTGGCGG ACCCCGCGCA GCCGTGCAGC GATCCACCAG CTTCGGGGTC
    CCCAACGCCA ACAGCATCAA GCAGATGCTG CTGGACTGGT GTCGAGCCAA GACTCGCGGC
    TACGAGCACG TCGACATCCA GAACTTCTCC TCCAGCTGGA GTGATGGGAT GGCCTTCTGT
    GCCCTGGTGC ACAACTTCTT CCCTGAGGCC TTCGACTATG GGCAGCTTAG CCCTCAGAAC
    CGACGCCAGA ACTTCGAGGT GGCCTTCTCA TCTGCGGAGA CCCATGCGGA CTGCCCGCAG
    CTCCTGGATA CAGAGGACAT GGTGCGGCTT CGAGAGCCTG ACTGGAAGTG CGTGTACACG
    TACATCCAGG AATTCTACCG CTGTCTGGTC CAGAAGGGGC TGGTAAAAAC CAAAAAGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.