SKP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SKP1
Entrez Gene ID	6500
Full Name	S-phase kinase associated protein 1
Synonyms	EMC19,OCP-II,OCP2,SKP1A,TCEB1L,p19A
General protein information	Preferred Names S-phase kinase associated protein 1 Names S-phase kinase-associated protein 1 OCP-2 RNA polymerase II elongation factor-like protein OCP2 SIII cyclin A/CDK2-associated p19 cyclin-A/CDK2-associated protein p19 organ of Corti protein 2 organ of Corti protein II p19skp1 transcription elongation factor B polypeptide 1-like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.1
Summary	This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Jul 2008].
Disorder MIM:	601434

mRNA and Protein(s)

mRNA	Protein	Name
NM_170679.2	NP_733779.1	S-phase kinase-associated protein 1 isoform b
NM_006930.3	NP_008861.2	S-phase kinase-associated protein 1 isoform a
NM_170679.3	NP_733779.1	S-phase kinase-associated protein 1 isoform b

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SKP1	NP_001239337.1
Drosophila melanogaster (fruit fly)	skpA	NP_726695.1
Caenorhabditis elegans (roundworm)	skr-1	NP_492513.1
Kluyveromyces lactis	KLLA0E16941g	XP_454713.1
Eremothecium gossypii	AGOS_ADR295C	NP_984392.1
Schizosaccharomyces pombe (fission yeast)	skp1	NP_595455.1
Xenopus tropicalis (tropical clawed frog)	skp1	NP_001016519.1
Gallus gallus (chicken)	SKP1	NP_001006153.1
Saccharomyces cerevisiae (baker's yeast)	SKP1	NP_010615.3
Homo sapiens (human)	SKP1	NP_733779.1
Pan troglodytes (chimpanzee)	SKP1	XP_003950673.1
Bos taurus (cattle)	SKP1	NP_001029953.1
Danio rerio (zebrafish)	skp1	NP_957037.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008719	XP_314827.2
Caenorhabditis elegans (roundworm)	skr-2	NP_492512.1
Magnaporthe oryzae (rice blast fungus)	MGG_04978	XP_003712477.1
Neurospora crassa	NCU08991	XP_959019.1
	LOC703550	XP_001094634.1
Mus musculus (house mouse)	Skp1a	NP_035673.3
Rattus norvegicus (Norway rat)	Skp1	NP_001007609.1
Oryza sativa (rice)	Os11g0456300	NP_001176525.1

Gene Ontology
Bibliography

Related articles in PubMed

NOTCH2 Hajdu-Cheney Mutations Escape SCF
Fukushima H, Shimizu K, Watahiki A, Hoshikawa S, Kosho T, Oba D, Sakano S, Arakaki M, Yamada A, Nagashima K, Okabe K, Fukumoto S, Jimi E, Bigas A, Nakayama KI, Nakayama K, Aoki Y, Wei W, Inuzuka H
Molecular cell68(4)645-658.e5(2017 Nov)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Segregation analysis revealed that variants c.475T>G in SKP1, c.671G>A in PROB1, and c.527G>A in IL17B in the 5q31.1-q35.3 linkage region, and c.850G>A in HKDC1 in the 10q22 locus completely segregated with the phenotype in the studied Keratoconus family
Title: Variants in SKP1, PROB1, and IL17B genes at keratoconus 5q31.1-q35.3 susceptibility locus identified by whole-exome sequencing.

Both the F-box domain of Skp2 and Skp1-Skp2 domain motions displaying preferential conformational control can together facilitate polyubiquitination of a wide variety of substrates.
Title: Molecular dynamics simulations elucidate the mode of protein recognition by Skp1 and the F-box domain in the SCF complex.

Skp1 is critical to lung cancer pathogenesis
Title: Skp1 in lung cancer: clinical significance and therapeutic efficacy of its small molecule inhibitors.

Data show that epithelial-mesenchymal transition (EMT)-transcription factors can be dynamically degraded by an atypical ubiquitin E3 ligase complex Skp1-Pam-Fbxo45 (SPFFbxo45).
Title: Atypical ubiquitin E3 ligase complex Skp1-Pam-Fbxo45 controls the core epithelial-to-mesenchymal transition-inducing transcription factors.

We discuss how these results can explain the rapid association of Cdc34 and Skp1-cullin-F-box ligase (SCF).
Title: Ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase (SCF) interact through multiple conformations.

The following SKP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SKP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28926	NM_170679.3 Latest version!	Homo sapiens S-phase kinase associated protein 1 (SKP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu28935	NM_006930.3 Latest version!	Homo sapiens S-phase kinase associated protein 1 (SKP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu28926	NM_170679.2	Homo sapiens S-phase kinase associated protein 1 (SKP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SKP1 ( NM_170679.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_733779.1 Homo sapiens S-phase kinase associated protein 1 (SKP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28926
Clone ID Related Accession (Same CDS sequence)	NM_170679.2 , NM_170679.3
Accession Version	NM_170679.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	S-phase kinase-associated protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI670085.1, Z47087.1, BC065730.1, AI028694.1, BQ632052.1 and AI025746.1. On Nov 17, 2007 this sequence version replaced NM_170679.1. Transcript Variant: This variant (2) utilizes an alternate splice site in the 3' coding region, compared to variant 1. This results in a frameshift and slightly longer protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.137235.1, SRR1660809.151371.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGCCTTCAA TTAAGTTGCA GAGTTCTGAT GGAGAGATAT TTGAAGTTGA TGTGGAAATT 
GCCAAACAAT CTGTGACTAT TAAGACCATG TTGGAAGATT TGGGAATGGA TGATGAAGGA 
GATGATGACC CAGTTCCTCT ACCAAATGTG AATGCAGCAA TATTAAAAAA GGTCATTCAG 
TGGTGCACCC ACCACAAGGA TGACCCTCCT CCTCCTGAAG ATGATGAGAA CAAAGAAAAG 
CGAACAGATG ATATCCCTGT TTGGGACCAA GAATTCCTGA AAGTTGACCA AGGAACACTT 
TTTGAACTCA TTCTGGCTGC AAACTACTTA GACATCAAAG GTTTGCTTGA TGTTACATGC 
AAGACTGTTG CCAATATGAT CAAGGGGAAA ACTCCTGAGG AGATTCGCAA GACCTTCAAT 
ATCAAAAATG ACTTTACTGA AGAGGAGGAA GCCCAGGTAC GCAAAGAGAA CCAGTGGTGT 
GAAGAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733779.1
CDS	180..671
Translation	MPSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQWCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK

Target ORF information:

RefSeq Version	NM_170679.2
Organism	Homo sapiens(human)
Definition	Homo sapiens S-phase kinase associated protein 1 (SKP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170679.2

ORF Insert Sequence:

ATGCCTTCAA TTAAGTTGCA GAGTTCTGAT GGAGAGATAT TTGAAGTTGA TGTGGAAATT 
GCCAAACAAT CTGTGACTAT TAAGACCATG TTGGAAGATT TGGGAATGGA TGATGAAGGA 
GATGATGACC CAGTTCCTCT ACCAAATGTG AATGCAGCAA TATTAAAAAA GGTCATTCAG 
TGGTGCACCC ACCACAAGGA TGACCCTCCT CCTCCTGAAG ATGATGAGAA CAAAGAAAAG 
CGAACAGATG ATATCCCTGT TTGGGACCAA GAATTCCTGA AAGTTGACCA AGGAACACTT 
TTTGAACTCA TTCTGGCTGC AAACTACTTA GACATCAAAG GTTTGCTTGA TGTTACATGC 
AAGACTGTTG CCAATATGAT CAAGGGGAAA ACTCCTGAGG AGATTCGCAA GACCTTCAAT 
ATCAAAAATG ACTTTACTGA AGAGGAGGAA GCCCAGGTAC GCAAAGAGAA CCAGTGGTGT 
GAAGAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKP1 ( NM_170679.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_733779.1 Homo sapiens S-phase kinase associated protein 1 (SKP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28926
Clone ID Related Accession (Same CDS sequence)	NM_170679.2 , NM_170679.3
Accession Version	NM_170679.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	S-phase kinase-associated protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from Z47087.1, BC065730.1, AI028694.1, BQ632052.1, AI025746.1 and AC011336.5. On Jun 2, 2019 this sequence version replaced NM_170679.2. Transcript Variant: This variant (2) utilizes an alternate splice site in the 3' coding region, compared to variant 1. This results in a frameshift and slightly longer protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.197332.1, SRR1803617.267145.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGCCTTCAA TTAAGTTGCA GAGTTCTGAT GGAGAGATAT TTGAAGTTGA TGTGGAAATT 
GCCAAACAAT CTGTGACTAT TAAGACCATG TTGGAAGATT TGGGAATGGA TGATGAAGGA 
GATGATGACC CAGTTCCTCT ACCAAATGTG AATGCAGCAA TATTAAAAAA GGTCATTCAG 
TGGTGCACCC ACCACAAGGA TGACCCTCCT CCTCCTGAAG ATGATGAGAA CAAAGAAAAG 
CGAACAGATG ATATCCCTGT TTGGGACCAA GAATTCCTGA AAGTTGACCA AGGAACACTT 
TTTGAACTCA TTCTGGCTGC AAACTACTTA GACATCAAAG GTTTGCTTGA TGTTACATGC 
AAGACTGTTG CCAATATGAT CAAGGGGAAA ACTCCTGAGG AGATTCGCAA GACCTTCAAT 
ATCAAAAATG ACTTTACTGA AGAGGAGGAA GCCCAGGTAC GCAAAGAGAA CCAGTGGTGT 
GAAGAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733779.1
CDS	97..588
Translation	MPSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQWCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK

Target ORF information:

RefSeq Version	NM_170679.3
Organism	Homo sapiens(human)
Definition	Homo sapiens S-phase kinase associated protein 1 (SKP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170679.3

ORF Insert Sequence:

ATGCCTTCAA TTAAGTTGCA GAGTTCTGAT GGAGAGATAT TTGAAGTTGA TGTGGAAATT 
GCCAAACAAT CTGTGACTAT TAAGACCATG TTGGAAGATT TGGGAATGGA TGATGAAGGA 
GATGATGACC CAGTTCCTCT ACCAAATGTG AATGCAGCAA TATTAAAAAA GGTCATTCAG 
TGGTGCACCC ACCACAAGGA TGACCCTCCT CCTCCTGAAG ATGATGAGAA CAAAGAAAAG 
CGAACAGATG ATATCCCTGT TTGGGACCAA GAATTCCTGA AAGTTGACCA AGGAACACTT 
TTTGAACTCA TTCTGGCTGC AAACTACTTA GACATCAAAG GTTTGCTTGA TGTTACATGC 
AAGACTGTTG CCAATATGAT CAAGGGGAAA ACTCCTGAGG AGATTCGCAA GACCTTCAAT 
ATCAAAAATG ACTTTACTGA AGAGGAGGAA GCCCAGGTAC GCAAAGAGAA CCAGTGGTGT 
GAAGAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKP1 ( NM_006930.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_008861.2 Homo sapiens S-phase kinase associated protein 1 (SKP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28935
Clone ID Related Accession (Same CDS sequence)	NM_006930.3
Accession Version	NM_006930.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	S-phase kinase-associated protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI670085.1, BC025673.1, BC065730.1, AI028694.1, BQ632052.1 and AI025746.1. On Nov 17, 2007 this sequence version replaced NM_006930.2. Transcript Variant: This variant (1) represents the longer transcript variant but encodes the shorter isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.29487.1, SRR1803613.212261.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGCCTTCAA TTAAGTTGCA GAGTTCTGAT GGAGAGATAT TTGAAGTTGA TGTGGAAATT 
GCCAAACAAT CTGTGACTAT TAAGACCATG TTGGAAGATT TGGGAATGGA TGATGAAGGA 
GATGATGACC CAGTTCCTCT ACCAAATGTG AATGCAGCAA TATTAAAAAA GGTCATTCAG 
TGGTGCACCC ACCACAAGGA TGACCCTCCT CCTCCTGAAG ATGATGAGAA CAAAGAAAAG 
CGAACAGATG ATATCCCTGT TTGGGACCAA GAATTCCTGA AAGTTGACCA AGGAACACTT 
TTTGAACTCA TTCTGGCTGC AAACTACTTA GACATCAAAG GTTTGCTTGA TGTTACATGC 
AAGACTGTTG CCAATATGAT CAAGGGGAAA ACTCCTGAGG AGATTCGCAA GACCTTCAAT 
ATCAAAAATG ACTTTACTGA AGAGGAGGAA GCCCAGGTAG GTAGCACACA GTTTTGTCTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008861.2
CDS	180..662
Translation	MPSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQWCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVGSTQFCL

Target ORF information:

RefSeq Version	NM_006930.3
Organism	Homo sapiens(human)
Definition	Homo sapiens S-phase kinase associated protein 1 (SKP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006930.3

ORF Insert Sequence:

ATGCCTTCAA TTAAGTTGCA GAGTTCTGAT GGAGAGATAT TTGAAGTTGA TGTGGAAATT 
GCCAAACAAT CTGTGACTAT TAAGACCATG TTGGAAGATT TGGGAATGGA TGATGAAGGA 
GATGATGACC CAGTTCCTCT ACCAAATGTG AATGCAGCAA TATTAAAAAA GGTCATTCAG 
TGGTGCACCC ACCACAAGGA TGACCCTCCT CCTCCTGAAG ATGATGAGAA CAAAGAAAAG 
CGAACAGATG ATATCCCTGT TTGGGACCAA GAATTCCTGA AAGTTGACCA AGGAACACTT 
TTTGAACTCA TTCTGGCTGC AAACTACTTA GACATCAAAG GTTTGCTTGA TGTTACATGC 
AAGACTGTTG CCAATATGAT CAAGGGGAAA ACTCCTGAGG AGATTCGCAA GACCTTCAAT 
ATCAAAAATG ACTTTACTGA AGAGGAGGAA GCCCAGGTAG GTAGCACACA GTTTTGTCTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NOTCH2 Hajdu-Cheney Mutations Escape SCF
Molecular cell68(4)645-658.e5(2017 Nov)
Fukushima H,Shimizu K,Watahiki A,Hoshikawa S,Kosho T,Oba D,Sakano S,Arakaki M,Yamada A,Nagashima K,Okabe K,Fukumoto S,Jimi E,Bigas A,Nakayama KI,Nakayama K,Aoki Y,Wei W,Inuzuka H

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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SKP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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