MRPL32 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPL32
Entrez Gene ID	64983
Full Name	mitochondrial ribosomal protein L32
Synonyms	HSPC283,L32mt,MRP-L32,bMRP-59b
General protein information	Preferred Names mitochondrial ribosomal protein L32 Names 39S ribosomal protein L32, mitochondrial mitochondrial large ribosomal subunit protein bL32m
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p14.1
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008].
Disorder MIM:	611839

mRNA and Protein(s)

mRNA	Protein	Name
NM_031903.2	NP_114109.1	39S ribosomal protein L32, mitochondrial precursor
NM_031903.3	NP_114109.1	39S ribosomal protein L32, mitochondrial precursor

Pathways from BioSystems

Homologies

	MRPL32	XP_001097622.1
Canis lupus familiaris (dog)	MRPL32	XP_533077.1
Rattus norvegicus (Norway rat)	Mrpl32	NP_001099586.1
Xenopus tropicalis (tropical clawed frog)	mrpl32	NP_001017237.1
Gallus gallus (chicken)	MRPL32	XP_418869.1
Danio rerio (zebrafish)	mrpl32	NP_001038905.1
Pan troglodytes (chimpanzee)	MRPL32	XP_528025.2
Homo sapiens (human)	MRPL32	NP_114109.1
Bos taurus (cattle)	MRPL32	NP_001033768.1
Mus musculus (house mouse)	Mrpl32	NP_083547.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V
Nature structural & molecular biology24(10)866-869(2017 Oct)

Structure of the large ribosomal subunit from human mitochondria.
Brown A, Amunts A, Bai XC, Sugimoto Y, Edwards PC, Murshudov G, Scheres SHW, Ramakrishnan V
Science (New York, N.Y.)346(6210)718-722(2014 Nov)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Transcriptome analysis of human gastric cancer.
Oh JH, Yang JO, Hahn Y, Kim MR, Byun SS, Jeon YJ, Kim JM, Song KS, Noh SM, Kim S, Yoo HS, Kim YS, Kim NS
Mammalian genome : official journal of the International Mammalian Genome Society16(12)942-54(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following MRPL32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPL32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31891	NM_031903.3 Latest version!	Homo sapiens mitochondrial ribosomal protein L32 (MRPL32), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu31891	NM_031903.2	Homo sapiens mitochondrial ribosomal protein L32 (MRPL32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPL32 ( NM_031903.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_114109.1 Homo sapiens mitochondrial ribosomal protein L32 (MRPL32), mRNA.

Price & Availability↑

CloneID	OHu31891
Clone ID Related Accession (Same CDS sequence)	NM_031903.2 , NM_031903.3
Accession Version	NM_031903.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	39S ribosomal protein L32, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB456673.1, AB049649.1, BM833110.1 and BU687817.1. On Sep 29, 2007 this sequence version replaced NM_031903.1. ##Evidence-Data-START## Transcript exon combination :: AB049649.1, SRR1163657.479905.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGG CCATGCTGGT CTTGGTGGTT TCGCCGTGGT CTGCGGCCCG GGGAGTGCTT 
CGAAACTACT GGGAGCGACT GCTACGGAAG CTTCCGCAGA GCCGGCCGGG CTTTCCCAGT 
CCTCCGTGGG GACCAGCATT AGCAGTACAG GGCCCAGCCA TGTTTACAGA GCCAGCAAAT 
GATACCAGTG GAAGTAAAGA GAATTCCAGC CTTTTGGACA GTATCTTTTG GATGGCAGCT 
CCCAAAAATA GACGCACCAT TGAAGTTAAC CGGTGTAGGA GAAGAAATCC GCAGAAGCTT 
ATTAAAGTTA AGAACAACAT AGACGTTTGT CCTGAATGTG GTCACCTGAA ACAGAAACAT 
GTCCTTTGTG CCTACTGCTA TGAAAAGGTG TGCAAGGAGA CTGCAGAAAT CAGACGACAG 
ATAGGGAAGC AAGAAGGGGG CCCTTTTAAG GCTCCCACCA TAGAGACTGT GGTGCTGTAC 
ACGGGAGAGA CACCGTCTGA ACAAGATCAG GGCAAGAGGA TCATTGAACG AGACAGAAAG 
CGACCATCCT GGTTCACCCA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114109.1
CDS	48..614
Translation	MALAMLVLVVSPWSAARGVLRNYWERLLRKLPQSRPGFPSPPWGPALAVQGPAMFTEPANDTSGSKENSSLLDSIFWMAAPKNRRTIEVNRCRRRNPQKLIKVKNNIDVCPECGHLKQKHVLCAYCYEKVCKETAEIRRQIGKQEGGPFKAPTIETVVLYTGETPSEQDQGKRIIERDRKRPSWFTQN

Target ORF information:

RefSeq Version	NM_031903.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L32 (MRPL32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031903.2

ORF Insert Sequence:

ATGGCGCTGG CCATGCTGGT CTTGGTGGTT TCGCCGTGGT CTGCGGCCCG GGGAGTGCTT 
CGAAACTACT GGGAGCGACT GCTACGGAAG CTTCCGCAGA GCCGGCCGGG CTTTCCCAGT 
CCTCCGTGGG GACCAGCATT AGCAGTACAG GGCCCAGCCA TGTTTACAGA GCCAGCAAAT 
GATACCAGTG GAAGTAAAGA GAATTCCAGC CTTTTGGACA GTATCTTTTG GATGGCAGCT 
CCCAAAAATA GACGCACCAT TGAAGTTAAC CGGTGTAGGA GAAGAAATCC GCAGAAGCTT 
ATTAAAGTTA AGAACAACAT AGACGTTTGT CCTGAATGTG GTCACCTGAA ACAGAAACAT 
GTCCTTTGTG CCTACTGCTA TGAAAAGGTG TGCAAGGAGA CTGCAGAAAT CAGACGACAG 
ATAGGGAAGC AAGAAGGGGG CCCTTTTAAG GCTCCCACCA TAGAGACTGT GGTGCTGTAC 
ACGGGAGAGA CACCGTCTGA ACAAGATCAG GGCAAGAGGA TCATTGAACG AGACAGAAAG 
CGACCATCCT GGTTCACCCA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL32 ( NM_031903.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_114109.1 Homo sapiens mitochondrial ribosomal protein L32 (MRPL32), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31891
Clone ID Related Accession (Same CDS sequence)	NM_031903.2 , NM_031903.3
Accession Version	NM_031903.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	39S ribosomal protein L32, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010132.5. On Nov 23, 2018 this sequence version replaced NM_031903.2. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.362047.1, AB049649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000223324.3/ ENSP00000223324.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCTGG CCATGCTGGT CTTGGTGGTT TCGCCGTGGT CTGCGGCCCG GGGAGTGCTT 
CGAAACTACT GGGAGCGACT GCTACGGAAG CTTCCGCAGA GCCGGCCGGG CTTTCCCAGT 
CCTCCGTGGG GACCAGCATT AGCAGTACAG GGCCCAGCCA TGTTTACAGA GCCAGCAAAT 
GATACCAGTG GAAGTAAAGA GAATTCCAGC CTTTTGGACA GTATCTTTTG GATGGCAGCT 
CCCAAAAATA GACGCACCAT TGAAGTTAAC CGGTGTAGGA GAAGAAATCC GCAGAAGCTT 
ATTAAAGTTA AGAACAACAT AGACGTTTGT CCTGAATGTG GTCACCTGAA ACAGAAACAT 
GTCCTTTGTG CCTACTGCTA TGAAAAGGTG TGCAAGGAGA CTGCAGAAAT CAGACGACAG 
ATAGGGAAGC AAGAAGGGGG CCCTTTTAAG GCTCCCACCA TAGAGACTGT GGTGCTGTAC 
ACGGGAGAGA CACCGTCTGA ACAAGATCAG GGCAAGAGGA TCATTGAACG AGACAGAAAG 
CGACCATCCT GGTTCACCCA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114109.1
CDS	12..578
Translation	MALAMLVLVVSPWSAARGVLRNYWERLLRKLPQSRPGFPSPPWGPALAVQGPAMFTEPANDTSGSKENSSLLDSIFWMAAPKNRRTIEVNRCRRRNPQKLIKVKNNIDVCPECGHLKQKHVLCAYCYEKVCKETAEIRRQIGKQEGGPFKAPTIETVVLYTGETPSEQDQGKRIIERDRKRPSWFTQN

Target ORF information:

RefSeq Version	NM_031903.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L32 (MRPL32), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031903.3

ORF Insert Sequence:

ATGGCGCTGG CCATGCTGGT CTTGGTGGTT TCGCCGTGGT CTGCGGCCCG GGGAGTGCTT 
CGAAACTACT GGGAGCGACT GCTACGGAAG CTTCCGCAGA GCCGGCCGGG CTTTCCCAGT 
CCTCCGTGGG GACCAGCATT AGCAGTACAG GGCCCAGCCA TGTTTACAGA GCCAGCAAAT 
GATACCAGTG GAAGTAAAGA GAATTCCAGC CTTTTGGACA GTATCTTTTG GATGGCAGCT 
CCCAAAAATA GACGCACCAT TGAAGTTAAC CGGTGTAGGA GAAGAAATCC GCAGAAGCTT 
ATTAAAGTTA AGAACAACAT AGACGTTTGT CCTGAATGTG GTCACCTGAA ACAGAAACAT 
GTCCTTTGTG CCTACTGCTA TGAAAAGGTG TGCAAGGAGA CTGCAGAAAT CAGACGACAG 
ATAGGGAAGC AAGAAGGGGG CCCTTTTAAG GCTCCCACCA TAGAGACTGT GGTGCTGTAC 
ACGGGAGAGA CACCGTCTGA ACAAGATCAG GGCAAGAGGA TCATTGAACG AGACAGAAAG 
CGACCATCCT GGTTCACCCA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Nature structural & molecular biology24(10)866-869(2017 Oct)
Brown A,Rathore S,Kimanius D,Aibara S,Bai XC,Rorbach J,Amunts A,Ramakrishnan V

Structure of the large ribosomal subunit from human mitochondria.
Science (New York, N.Y.)346(6210)718-722(2014 Nov)
Brown A,Amunts A,Bai XC,Sugimoto Y,Edwards PC,Murshudov G,Scheres SHW,Ramakrishnan V

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Transcriptome analysis of human gastric cancer.
Mammalian genome : official journal of the International Mammalian Genome Society16(12)942-54(2005 Dec)
Oh JH,Yang JO,Hahn Y,Kim MR,Byun SS,Jeon YJ,Kim JM,Song KS,Noh SM,Kim S,Yoo HS,Kim YS,Kim NS

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MRPL32 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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