SKIL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SKIL
Entrez Gene ID	6498
Full Name	SKI like proto-oncogene
Synonyms	SNO,SnoA,SnoI,SnoN
General protein information	Preferred Names SKI like proto-oncogene Names ski-like protein SKI-like oncogene ski-related oncogene snoN
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q26.2
Summary	The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].
Disorder MIM:	165340

mRNA and Protein(s)

mRNA	Protein	Name
NM_001145098.3	NP_001138570.1	ski-like protein isoform 3
NM_005414.4	NP_005405.2	ski-like protein isoform 1
NM_005414.5	NP_005405.2	ski-like protein isoform 1
NM_001248008.1	NP_001234937.1	ski-like protein isoform 1
XM_006713735.1	XP_006713798.1	ski-like protein isoform X1
NM_001145098.2	NP_001138570.1	ski-like protein isoform 3
NM_001145097.2	NP_001138569.1	ski-like protein isoform 2
XM_005247721.1	XP_005247778.1	ski-like protein isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SKIL	NP_001192876.1
Rattus norvegicus (Norway rat)	Skil	XP_006232299.1
Gallus gallus (chicken)	SKIL	NP_990505.1
Pan troglodytes (chimpanzee)	SKIL	XP_001163447.1
Canis lupus familiaris (dog)	SKIL	XP_545283.2
Mus musculus (house mouse)	Skil	NP_035516.2
Danio rerio (zebrafish)	LOC100007653	XP_005171386.1
	SKIL	XP_001085298.2
Homo sapiens (human)	SKIL	NP_001234937.1
Xenopus tropicalis (tropical clawed frog)	skil	XP_002931592.1

Gene Ontology
Bibliography

Related articles in PubMed

Down-regulation of miR-23a inhibits high glucose-induced EMT and renal fibrogenesis by up-regulation of SnoN.
Xu H, Sun F, Li X, Sun L
Human cell31(1)22-32(2018 Jan)

SnoN Stabilizes the SMAD3/SMAD4 Protein Complex.
Walld?n K, Nyman T, H?llberg BM
Scientific reports746370(2017 Apr)

Repression of Smad3 by Stat3 and c-Ski/SnoN induces gefitinib resistance in lung adenocarcinoma.
Makino Y, Yoon JH, Bae E, Kato M, Miyazawa K, Ohira T, Ikeda N, Kuroda M, Mamura M
Biochemical and biophysical research communications484(2)269-277(2017 03)

SnoN suppresses TGF-?-induced epithelial-mesenchymal transition and invasion of bladder cancer in a TIF1?-dependent manner.
Yin X, Xu C, Zheng X, Yuan H, Liu M, Qiu Y, Chen J
Oncology reports36(3)1535-41(2016 Sep)

SnoN Antagonizes the Hippo Kinase Complex to Promote TAZ Signaling during Breast Carcinogenesis.
Zhu Q, Le Scolan E, Jahchan N, Ji X, Xu A, Luo K
Developmental cell37(5)399-412(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

signal transducer and activator of transcription (Stat)3 represses Smad3 in synergy with the potent negative regulators of TGF-beta signaling, c-Ski and SnoN, whereby renders gefitinib-sensitive HCC827 cells resistant
Title: Repression of Smad3 by Stat3 and c-Ski/SnoN induces gefitinib resistance in lung adenocarcinoma.

suggest that SnoN suppresses TGF-betainduced epithelial-mesenchymal transition and invasion of bladder cancer cells in a TIF1gammadependent manner
Title: SnoN suppresses TGF-?-induced epithelial-mesenchymal transition and invasion of bladder cancer in a TIF1?-dependent manner.

SnoN interacts with multiple components of the Hippo pathway to inhibit the binding of Lats2 to TAZ and the subsequent phosphorylation of TAZ, leading to TAZ stabilization.
Title: SnoN Antagonizes the Hippo Kinase Complex to Promote TAZ Signaling during Breast Carcinogenesis.

the findings of this study demonstrate that the downregulation of SnoN expression in hRPTECs under high-glucose conditions is mediated by the increased expression of Smurf2 through the TGF-b1/Smad signaling pathway.
Title: The downregulation of SnoN expression in human renal proximal tubule epithelial cells under high-glucose conditions is mediated by an increase in Smurf2 expression through TGF-?1 signaling.

RNAi-mediated downregulation of SnoN effectively inhibited proliferation and enhanced apoptosis of pancreatic cells.
Title: The influence of SnoN gene silencing by siRNA on the cell proliferation and apoptosis of human pancreatic cancer cells.

The following SKIL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SKIL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28518	NM_001145097.2 Latest version!	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu28540	NM_001145098.3 Latest version!	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu28538	NM_001248008.1 Latest version!	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu28538	NM_005414.5 Latest version!	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu28538	XM_005247721.1 Latest version!	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu28538	XM_006713735.1 Latest version!	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu28538	NM_005414.4	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu28540	NM_001145098.2	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SKIL ( NM_001145097.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138569.1 Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28518
Clone ID Related Accession (Same CDS sequence)	NM_001145097.2
Accession Version	NM_001145097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-30
Organism	Homo sapiens(human)
Product	ski-like protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U70730.1, AC073288.27 and AI245308.1. On Oct 15, 2011 this sequence version replaced NM_001145097.1. Transcript Variant: This variant (2, also known as SnoN2) differs in the 5' UTR and uses an alternate in-frame splice site in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U70730.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AATGCATCAA TCTCAAATAA TTCTACAAGT 
AAAAGGAAAT CTGAGTCTGC CACTTGCAAC TTAGTCAGAG ACATAAACAA AGTGGGAATT 
GGCCTTGTTG CTGCCGCTTC ATCTCCGCTT CTTGTGAAAG ATGTCATTTG TGAGGATGAT 
AAGGGAAAAA TCATGGAAGA AGTAATGAGA ACTTATTTAA AACAACAGGA AAAACTAAAC 
TTGATTTTGC AAAAGAAGCA ACAACTTCAG ATGGAAGTAA AAATGTTGAG TAGTTCAAAA 
TCTATGAAGG AACTCACTGA AGAACAGCAG AATTTACAGA AAGAGCTTGA ATCTTTGCAG 
AATGAACATG CTCAAAGAAT GGAAGAATTT TATGTTGAAC AGAAAGACTT AGAGAAAAAA 
TTGGAGCAGA TAATGAAGCA AAAATGTACC TGTGACTCAA ATTTAGAAAA AGACAAAGAG 
GCTGAATATG CAGGACAGTT GGCAGAACTG AGGCAGAGAT TGGACCATGC TGAGGCCGAT 
AGGCAAGAAC TCCAAGATGA ACTCAGACAG GAACGGGAAG CAAGACAGAA GTTAGAGATG 
ATGATAAAAG AGCTAAAGCT GCAAATTCTG AAATCATCAA AGACTGCTAA AGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138569.1
CDS	710..2626
Translation	MENLQTNFSLVQGSTKKLNGMGDDGSPPAKKMITDIHANGKTINKVPTVKKEHLDDYGEAPVETDGEHVKRTCTSVPETLHLNPSLKHTLAQFHLSSQSSLGGPAAFSARHSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTVLEGESISCFQVGGEKRLCLPQVLNSVLREFTLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSVLPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEANASISNNSTSKRKSESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVKMLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYAGQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE

Target ORF information:

RefSeq Version	NM_001145097.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145097.2

ORF Insert Sequence:

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AATGCATCAA TCTCAAATAA TTCTACAAGT 
AAAAGGAAAT CTGAGTCTGC CACTTGCAAC TTAGTCAGAG ACATAAACAA AGTGGGAATT 
GGCCTTGTTG CTGCCGCTTC ATCTCCGCTT CTTGTGAAAG ATGTCATTTG TGAGGATGAT 
AAGGGAAAAA TCATGGAAGA AGTAATGAGA ACTTATTTAA AACAACAGGA AAAACTAAAC 
TTGATTTTGC AAAAGAAGCA ACAACTTCAG ATGGAAGTAA AAATGTTGAG TAGTTCAAAA 
TCTATGAAGG AACTCACTGA AGAACAGCAG AATTTACAGA AAGAGCTTGA ATCTTTGCAG 
AATGAACATG CTCAAAGAAT GGAAGAATTT TATGTTGAAC AGAAAGACTT AGAGAAAAAA 
TTGGAGCAGA TAATGAAGCA AAAATGTACC TGTGACTCAA ATTTAGAAAA AGACAAAGAG 
GCTGAATATG CAGGACAGTT GGCAGAACTG AGGCAGAGAT TGGACCATGC TGAGGCCGAT 
AGGCAAGAAC TCCAAGATGA ACTCAGACAG GAACGGGAAG CAAGACAGAA GTTAGAGATG 
ATGATAAAAG AGCTAAAGCT GCAAATTCTG AAATCATCAA AGACTGCTAA AGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIL ( NM_001145098.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138570.1 Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28540
Clone ID Related Accession (Same CDS sequence)	NM_001145098.3 , NM_001145098.2
Accession Version	NM_001145098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-30
Organism	Homo sapiens(human)
Product	ski-like protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA464211.1, AK300053.1, AC073288.27 and AI245308.1. On May 31, 2019 this sequence version replaced NM_001145098.2. Transcript Variant: This variant (3) differs in the 5' UTR and coding region, and uses a downstream start codon compared to variant 1. The resulting protein (isoform 3) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300053.1, SRR1803613.111760.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138570.1
CDS	252..2246
Translation	MGDDGSPPAKKMITDIHANGKTINKVPTVKKEHLDDYGEAPVETDGEHVKRTCTSVPETLHLNPSLKHTLAQFHLSSQSSLGGPAAFSARHSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTVLEGESISCFQVGGEKRLCLPQVLNSVLREFTLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSVLPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEASKSISRQSEKAHSSGKLQKTVSYPDVSLEEQEKMDLKTSRELCSRLDASISNNSTSKRKSESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVKMLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYAGQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE

Target ORF information:

RefSeq Version	NM_001145098.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145098.3

ORF Insert Sequence:

ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIL ( NM_001145098.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138570.1 Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28540
Clone ID Related Accession (Same CDS sequence)	NM_001145098.3 , NM_001145098.2
Accession Version	NM_001145098.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	ski-like protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA464211.1, AK300053.1, AC073288.27 and AI245308.1. On Oct 15, 2011 this sequence version replaced NM_001145098.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding region, and uses a downstream start codon compared to variant 1. The resulting protein (isoform 3) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.111760.1, AK300053.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138570.1
CDS	282..2276
Translation	MGDDGSPPAKKMITDIHANGKTINKVPTVKKEHLDDYGEAPVETDGEHVKRTCTSVPETLHLNPSLKHTLAQFHLSSQSSLGGPAAFSARHSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTVLEGESISCFQVGGEKRLCLPQVLNSVLREFTLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSVLPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEASKSISRQSEKAHSSGKLQKTVSYPDVSLEEQEKMDLKTSRELCSRLDASISNNSTSKRKSESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVKMLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYAGQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE

Target ORF information:

RefSeq Version	NM_001145098.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145098.2

ORF Insert Sequence:

ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIL ( NM_005414.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005405.2 Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28538
Clone ID Related Accession (Same CDS sequence)	NM_005414.4 , NM_005414.5 , NM_001248008.1 , XM_006713735.1 , XM_005247721.1
Accession Version	NM_005414.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-28
Organism	Homo sapiens(human)
Product	ski-like protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA464211.1, AC073288.27, BC059386.1, X15219.1 and AI245308.1. This sequence is a reference standard in the RefSeqGene project. On Oct 15, 2011 this sequence version replaced NM_005414.3. Transcript Variant: This variant (1) encodes the longest isoform (1, also known as snoN). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.112482.1, SRR1660809.15608.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005405.2
CDS	713..2767
Translation	MENLQTNFSLVQGSTKKLNGMGDDGSPPAKKMITDIHANGKTINKVPTVKKEHLDDYGEAPVETDGEHVKRTCTSVPETLHLNPSLKHTLAQFHLSSQSSLGGPAAFSARHSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTVLEGESISCFQVGGEKRLCLPQVLNSVLREFTLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSVLPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEASKSISRQSEKAHSSGKLQKTVSYPDVSLEEQEKMDLKTSRELCSRLDASISNNSTSKRKSESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVKMLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYAGQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE

Target ORF information:

RefSeq Version	NM_005414.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005414.4

ORF Insert Sequence:

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIL ( NM_005414.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005405.2 Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28538
Clone ID Related Accession (Same CDS sequence)	NM_005414.4 , NM_005414.5 , NM_001248008.1 , XM_006713735.1 , XM_005247721.1
Accession Version	NM_005414.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	ski-like protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA464211.1, AC073288.27, BC059386.1, X15219.1 and AI245308.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005414.4. Transcript Variant: This variant (1) encodes the longest isoform (1, also known as snoN). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.112482.1, SRR1660809.15608.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000259119.9/ ENSP00000259119.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005405.2
CDS	683..2737
Translation	MENLQTNFSLVQGSTKKLNGMGDDGSPPAKKMITDIHANGKTINKVPTVKKEHLDDYGEAPVETDGEHVKRTCTSVPETLHLNPSLKHTLAQFHLSSQSSLGGPAAFSARHSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTVLEGESISCFQVGGEKRLCLPQVLNSVLREFTLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSVLPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEASKSISRQSEKAHSSGKLQKTVSYPDVSLEEQEKMDLKTSRELCSRLDASISNNSTSKRKSESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVKMLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYAGQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE

Target ORF information:

RefSeq Version	NM_005414.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005414.5

ORF Insert Sequence:

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIL ( NM_001248008.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001234937.1 Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 4, mRNA.

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CloneID	OHu28538
Clone ID Related Accession (Same CDS sequence)	NM_005414.4 , NM_005414.5 , NM_001248008.1 , XM_006713735.1 , XM_005247721.1
Accession Version	NM_001248008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-30
Organism	Homo sapiens(human)
Product	ski-like protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X15219.1, AC073288.27 and AI245308.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X15219.1, SRR1803612.88411.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001234937.1
CDS	710..2764
Translation	MENLQTNFSLVQGSTKKLNGMGDDGSPPAKKMITDIHANGKTINKVPTVKKEHLDDYGEAPVETDGEHVKRTCTSVPETLHLNPSLKHTLAQFHLSSQSSLGGPAAFSARHSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTVLEGESISCFQVGGEKRLCLPQVLNSVLREFTLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSVLPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEASKSISRQSEKAHSSGKLQKTVSYPDVSLEEQEKMDLKTSRELCSRLDASISNNSTSKRKSESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVKMLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYAGQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE

Target ORF information:

RefSeq Version	NM_001248008.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001248008.1

ORF Insert Sequence:

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIL ( XM_006713735.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713798.1 Homo sapiens SKI like proto-oncogene (SKIL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu28538
Clone ID Related Accession (Same CDS sequence)	NM_005414.4 , NM_005414.5 , NM_001248008.1 , XM_006713735.1 , XM_005247721.1
Accession Version	XM_006713735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ski-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713798.1
CDS	813..2867
Translation	MENLQTNFSLVQGSTKKLNGMGDDGSPPAKKMITDIHANGKTINKVPTVKKEHLDDYGEAPVETDGEHVKRTCTSVPETLHLNPSLKHTLAQFHLSSQSSLGGPAAFSARHSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTVLEGESISCFQVGGEKRLCLPQVLNSVLREFTLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSVLPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEASKSISRQSEKAHSSGKLQKTVSYPDVSLEEQEKMDLKTSRELCSRLDASISNNSTSKRKSESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVKMLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYAGQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE

Target ORF information:

RefSeq Version	XM_006713735.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713735.1

ORF Insert Sequence:

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIL ( XM_005247721.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247778.1 Homo sapiens SKI like proto-oncogene (SKIL), transcript variant X1, mRNA.

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CloneID	OHu28538
Clone ID Related Accession (Same CDS sequence)	NM_005414.4 , NM_005414.5 , NM_001248008.1 , XM_006713735.1 , XM_005247721.1
Accession Version	XM_005247721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ski-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247778.1
CDS	744..2798
Translation	MENLQTNFSLVQGSTKKLNGMGDDGSPPAKKMITDIHANGKTINKVPTVKKEHLDDYGEAPVETDGEHVKRTCTSVPETLHLNPSLKHTLAQFHLSSQSSLGGPAAFSARHSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTVLEGESISCFQVGGEKRLCLPQVLNSVLREFTLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSVLPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEASKSISRQSEKAHSSGKLQKTVSYPDVSLEEQEKMDLKTSRELCSRLDASISNNSTSKRKSESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVKMLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYAGQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE

Target ORF information:

RefSeq Version	XM_005247721.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SKI like proto-oncogene (SKIL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005247721.1

ORF Insert Sequence:

ATGGAAAACC TCCAGACAAA TTTCTCCTTG GTTCAGGGCT CAACTAAAAA ACTGAATGGG 
ATGGGAGATG ATGGCAGCCC CCCAGCGAAA AAAATGATAA CGGACATTCA TGCAAATGGA 
AAAACGATAA ACAAGGTGCC AACAGTTAAG AAGGAACACT TGGATGACTA TGGAGAAGCA 
CCAGTGGAAA CTGATGGAGA GCATGTTAAG CGAACCTGTA CTTCTGTTCC TGAAACTTTG 
CATTTAAATC CCAGTTTGAA ACACACATTG GCACAATTCC ATTTAAGTAG TCAGAGCTCG 
CTGGGTGGAC CAGCAGCATT TTCTGCTCGG CATTCCCAAG AAAGCATGTC GCCTACTGTA 
TTTCTGCCTC TTCCATCACC TCAGGTTCTT CCTGGCCCAT TGCTCATCCC TTCAGATAGC 
TCCACAGAAC TCACTCAGAC TGTGTTGGAA GGGGAATCTA TTTCTTGTTT TCAAGTTGGA 
GGAGAAAAGA GACTCTGTTT GCCCCAAGTC TTAAATTCTG TTCTCCGAGA ATTTACACTC 
CAGCAAATAA ATACAGTGTG TGATGAACTG TACATATATT GTTCAAGGTG TACTTCAGAC 
CAGCTTCATA TCTTAAAGGT ACTGGGCATA CTTCCATTCA ATGCCCCATC CTGTGGGCTG 
ATTACATTAA CTGATGCACA AAGATTATGT AATGCTTTAT TGCGGCCACG AACTTTTCCT 
CAAAATGGTA GCGTACTTCC TGCTAAAAGC TCATTGGCCC AGTTAAAGGA AACTGGCAGT 
GCCTTTGAAG TGGAGCATGA ATGCCTAGGC AAATGTCAGG GTTTATTTGC ACCCCAGTTT 
TATGTTCAGC CTGATGCTCC GTGTATTCAA TGTCTGGAGT GTTGTGGAAT GTTTGCACCC 
CAGACGTTTG TGATGCATTC TCACAGATCA CCTGACAAAA GAACTTGCCA CTGGGGCTTT 
GAATCAGCTA AATGGCATTG CTATCTTCAT GTGAACCAAA AATACTTAGG AACACCTGAA 
GAAAAGAAAC TGAAGATAAT TTTAGAAGAA ATGAAGGAGA AGTTTAGCAT GAGAAGTGGA 
AAGAGAAATC AATCCAAGAC AGATGCACCA TCAGGAATGG AATTACAGTC ATGGTATCCT 
GTTATAAAGC AGGAAGGTGA CCATGTTTCT CAGACACATT CATTTTTACA CCCCAGCTAC 
TACTTATACA TGTGTGATAA AGTGGTTGCC CCAAATGTGT CACTTACTTC TGCTGTATCC 
CAGTCTAAAG AGCTCACAAA GACAGAGGCA AGTAAGTCCA TATCAAGACA GTCAGAGAAG 
GCTCACAGTA GTGGTAAACT TCAAAAAACA GTGTCTTATC CAGATGTCTC ACTTGAGGAA 
CAGGAGAAAA TGGATTTAAA AACAAGTAGA GAATTATGTA GCCGTTTAGA TGCATCAATC 
TCAAATAATT CTACAAGTAA AAGGAAATCT GAGTCTGCCA CTTGCAACTT AGTCAGAGAC 
ATAAACAAAG TGGGAATTGG CCTTGTTGCT GCCGCTTCAT CTCCGCTTCT TGTGAAAGAT 
GTCATTTGTG AGGATGATAA GGGAAAAATC ATGGAAGAAG TAATGAGAAC TTATTTAAAA 
CAACAGGAAA AACTAAACTT GATTTTGCAA AAGAAGCAAC AACTTCAGAT GGAAGTAAAA 
ATGTTGAGTA GTTCAAAATC TATGAAGGAA CTCACTGAAG AACAGCAGAA TTTACAGAAA 
GAGCTTGAAT CTTTGCAGAA TGAACATGCT CAAAGAATGG AAGAATTTTA TGTTGAACAG 
AAAGACTTAG AGAAAAAATT GGAGCAGATA ATGAAGCAAA AATGTACCTG TGACTCAAAT 
TTAGAAAAAG ACAAAGAGGC TGAATATGCA GGACAGTTGG CAGAACTGAG GCAGAGATTG 
GACCATGCTG AGGCCGATAG GCAAGAACTC CAAGATGAAC TCAGACAGGA ACGGGAAGCA 
AGACAGAAGT TAGAGATGAT GATAAAAGAG CTAAAGCTGC AAATTCTGAA ATCATCAAAG 
ACTGCTAAAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Down-regulation of miR-23a inhibits high glucose-induced EMT and renal fibrogenesis by up-regulation of SnoN.
Human cell31(1)22-32(2018 Jan)
Xu H,Sun F,Li X,Sun L

SnoN Stabilizes the SMAD3/SMAD4 Protein Complex.
Scientific reports746370(2017 Apr)
Walld?n K,Nyman T,H?llberg BM

Repression of Smad3 by Stat3 and c-Ski/SnoN induces gefitinib resistance in lung adenocarcinoma.
Biochemical and biophysical research communications484(2)269-277(2017 03)
Makino Y,Yoon JH,Bae E,Kato M,Miyazawa K,Ohira T,Ikeda N,Kuroda M,Mamura M

SnoN suppresses TGF-?-induced epithelial-mesenchymal transition and invasion of bladder cancer in a TIF1?-dependent manner.
Oncology reports36(3)1535-41(2016 Sep)
Yin X,Xu C,Zheng X,Yuan H,Liu M,Qiu Y,Chen J

SnoN Antagonizes the Hippo Kinase Complex to Promote TAZ Signaling during Breast Carcinogenesis.
Developmental cell37(5)399-412(2016 Jun)
Zhu Q,Le Scolan E,Jahchan N,Ji X,Xu A,Luo K

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SKIL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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