CENPH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CENPH
Entrez Gene ID	64946
Full Name	centromere protein H
General protein information	Preferred Names centromere protein H Names centromere protein H CENP-H interphase centromere complex protein 35 kinetochore protein CENP-H
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q13.2
Summary	Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008].
Disorder MIM:	605607

mRNA and Protein(s)

mRNA	Protein	Name
NM_022909.4	NP_075060.1	centromere protein H
NM_022909.3	NP_075060.1	centromere protein H

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CENPH	XP_005617514.1
Mus musculus (house mouse)	Cenph	NP_068686.1
Homo sapiens (human)	CENPH	NP_075060.1
Bos taurus (cattle)	CENPH	NP_001029408.1
Xenopus tropicalis (tropical clawed frog)	cenph	XP_002934249.2
Pan troglodytes (chimpanzee)	CENPH	XP_001145587.2
	CENPH	XP_001091765.1
Rattus norvegicus (Norway rat)	LOC681185	XP_006231927.1
Gallus gallus (chicken)	CENPH	NP_989942.1

Gene Ontology
Bibliography

Related articles in PubMed

CENP-H regulates the cell growth of human hepatocellular carcinoma cells through the mitochondrial apoptotic pathway.
Lu G, Hou H, Lu X, Ke X, Wang X, Zhang D, Zhao Y, Zhang J, Ren M, He S
Oncology reports37(6)3484-3492(2017 Jun)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis.
Zhu L, Wang Z, Wang W, Wang C, Hua S, Su Z, Brako L, Garcia-Barrio M, Ye M, Wei X, Zou H, Ding X, Liu L, Liu X, Yao X
The Journal of biological chemistry290(45)27053-66(2015 Nov)

Upregulation of centromere protein H is associated with progression of renal cell carcinoma.
Wu X, Lin Y, Shi L, Huang Y, Lai C, Wang Y, Zhang M, Wang S, Heng B, Yu G, Du X, Fang L, Fu Y, Chen J, Guo Z, Su Z, Wu S
Journal of molecular histology46(4-5)377-85(2015 Oct)

Overexpression of CENP-H as a novel prognostic biomarker for human hepatocellular carcinoma progression and patient survival.
Lu G, Shan T, He S, Ren M, Zhu M, Hu Y, Lu X, Zhang D
Oncology reports30(5)2238-44(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

propose that CSPP1 cooperates with CENP-H on kinetochores to serve as a novel regulator of kinetochore microtubule dynamics for accurate chromosome segregation
Title: Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis.

Upregulation of centromere protein H is associated with progression of renal cell carcinoma
Title: Upregulation of centromere protein H is associated with progression of renal cell carcinoma.

CENP-H was overexpressed in HCC, and its level of upregulation was an independent prognostic indicator, suggesting that CENP-H may be an effective therapeutic strategy for the treatment of HCC.
Title: Overexpression of CENP-H as a novel prognostic biomarker for human hepatocellular carcinoma progression and patient survival.

High expression of CENP-H in gastric cancer indicates poor prognosis and Survivin may mediate its procancer role.
Title: Combined evaluation of centromere protein H and Ki-67 as prognostic biomarker for patients with gastric carcinoma.

Sp1 and Sp3 bind to the CENPH minimal promoter and function as a regulator of the transcription of CENPH in nasopharyngeal carcinomas.
Title: Sp1 and Sp3 are involved in the full transcriptional activity of centromere protein H in human nasopharyngeal carcinoma cells.

The following CENPH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CENPH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15777	NM_022909.4 Latest version!	Homo sapiens centromere protein H (CENPH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu15777	NM_022909.3	Homo sapiens centromere protein H (CENPH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CENPH ( NM_022909.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_075060.1 Homo sapiens centromere protein H (CENPH), mRNA.

Price & Availability↑

CloneID	OHu15777
Clone ID Related Accession (Same CDS sequence)	NM_022909.4 , NM_022909.3
Accession Version	NM_022909.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	centromere protein H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022107.8. On Nov 26, 2018 this sequence version replaced NM_022909.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.400574.1, BC012024.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000283006.7/ ENSP00000283006.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGAGC AGCCCCAGAT GCAAGACGCC GACGAGCCCG CGGACTCCGG AGGGGAAGGC 
CGGGCAGGCG GGCCACCGCA GGTCGCCGGC GCCCAGGCGG CGTGCAGCGA GGACCGCATG 
ACCCTGCTCC TCAGGCTGAG AGCACAGACA AAACAACAAC TCTTAGAATA TAAATCAATG 
GTTGATGCAA GTGAAGAAAA AACTCCAGAA CAAATTATGC AAGAAAAGCA AATCGAAGCT 
AAAATTGAAG ACCTGGAAAA TGAAATTGAA GAGGTAAAAG TTGCTTTTGA GATAAAAAAG 
CTTGCATTAG ACAGGATGAG ACTTTCAACT GCACTTAAAA AAAACCTGGA GAAAATTAGC 
AGACAGTCTA GTGTGCTCAT GGATAACATG AAACACCTAT TAGAGCTAAA TAAATTAATA 
ATGAAATCAC AGCAGGAATC TTGGGATTTA GAGGAAAAAC TGCTTGATAT TAGAAAGAAG 
AGATTGCAAT TAAAACAAGC TTCAGAAAGT AAGCTTTTAG AAATACAGAC TGAAAAGAAC 
AAACAGAAGA TTGATTTGGA CAGTATGGAA AACTCAGAGA GGATAAAGAT CATACGACAA 
AACCTACAGA TGGAGATAAA AATTACTACT GTTATTCAAC ATGTGTTCCA GAACCTTATT 
TTGGGGAGTA AAGTCAATTG GGCAGAGGAT CCTGCCCTTA AGGAAATTGT TCTGCAGCTT 
GAGAAGAATG TTGACATGAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075060.1
CDS	53..796
Translation	MEEQPQMQDADEPADSGGEGRAGGPPQVAGAQAACSEDRMTLLLRLRAQTKQQLLEYKSMVDASEEKTPEQIMQEKQIEAKIEDLENEIEEVKVAFEIKKLALDRMRLSTALKKNLEKISRQSSVLMDNMKHLLELNKLIMKSQQESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKNKQKIDLDSMENSERIKIIRQNLQMEIKITTVIQHVFQNLILGSKVNWAEDPALKEIVLQLEKNVDMM

Target ORF information:

RefSeq Version	NM_022909.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein H (CENPH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022909.4

ORF Insert Sequence:

ATGGAGGAGC AGCCCCAGAT GCAAGACGCC GACGAGCCCG CGGACTCCGG AGGGGAAGGC 
CGGGCAGGCG GGCCACCGCA GGTCGCCGGC GCCCAGGCGG CGTGCAGCGA GGACCGCATG 
ACCCTGCTCC TCAGGCTGAG AGCACAGACA AAACAACAAC TCTTAGAATA TAAATCAATG 
GTTGATGCAA GTGAAGAAAA AACTCCAGAA CAAATTATGC AAGAAAAGCA AATCGAAGCT 
AAAATTGAAG ACCTGGAAAA TGAAATTGAA GAGGTAAAAG TTGCTTTTGA GATAAAAAAG 
CTTGCATTAG ACAGGATGAG ACTTTCAACT GCACTTAAAA AAAACCTGGA GAAAATTAGC 
AGACAGTCTA GTGTGCTCAT GGATAACATG AAACACCTAT TAGAGCTAAA TAAATTAATA 
ATGAAATCAC AGCAGGAATC TTGGGATTTA GAGGAAAAAC TGCTTGATAT TAGAAAGAAG 
AGATTGCAAT TAAAACAAGC TTCAGAAAGT AAGCTTTTAG AAATACAGAC TGAAAAGAAC 
AAACAGAAGA TTGATTTGGA CAGTATGGAA AACTCAGAGA GGATAAAGAT CATACGACAA 
AACCTACAGA TGGAGATAAA AATTACTACT GTTATTCAAC ATGTGTTCCA GAACCTTATT 
TTGGGGAGTA AAGTCAATTG GGCAGAGGAT CCTGCCCTTA AGGAAATTGT TCTGCAGCTT 
GAGAAGAATG TTGACATGAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPH ( NM_022909.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_075060.1 Homo sapiens centromere protein H (CENPH), mRNA.

Price & Availability↑

CloneID	OHu15777
Clone ID Related Accession (Same CDS sequence)	NM_022909.4 , NM_022909.3
Accession Version	NM_022909.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	centromere protein H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015355.1. On May 30, 2002 this sequence version replaced NM_022909.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015355.1, SRR1163658.9346.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGC AGCCCCAGAT GCAAGACGCC GACGAGCCCG CGGACTCCGG AGGGGAAGGC 
CGGGCAGGCG GGCCACCGCA GGTCGCCGGC GCCCAGGCGG CGTGCAGCGA GGACCGCATG 
ACCCTGCTCC TCAGGCTGAG AGCACAGACA AAACAACAAC TCTTAGAATA TAAATCAATG 
GTTGATGCAA GTGAAGAAAA AACTCCAGAA CAAATTATGC AAGAAAAGCA AATCGAAGCT 
AAAATTGAAG ACCTGGAAAA TGAAATTGAA GAGGTAAAAG TTGCTTTTGA GATAAAAAAG 
CTTGCATTAG ACAGGATGAG ACTTTCAACT GCACTTAAAA AAAACCTGGA GAAAATTAGC 
AGACAGTCTA GTGTGCTCAT GGATAACATG AAACACCTAT TAGAGCTAAA TAAATTAATA 
ATGAAATCAC AGCAGGAATC TTGGGATTTA GAGGAAAAAC TGCTTGATAT TAGAAAGAAG 
AGATTGCAAT TAAAACAAGC TTCAGAAAGT AAGCTTTTAG AAATACAGAC TGAAAAGAAC 
AAACAGAAGA TTGATTTGGA CAGTATGGAA AACTCAGAGA GGATAAAGAT CATACGACAA 
AACCTACAGA TGGAGATAAA AATTACTACT GTTATTCAAC ATGTGTTCCA GAACCTTATT 
TTGGGGAGTA AAGTCAATTG GGCAGAGGAT CCTGCCCTTA AGGAAATTGT TCTGCAGCTT 
GAGAAGAATG TTGACATGAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075060.1
CDS	88..831
Translation	MEEQPQMQDADEPADSGGEGRAGGPPQVAGAQAACSEDRMTLLLRLRAQTKQQLLEYKSMVDASEEKTPEQIMQEKQIEAKIEDLENEIEEVKVAFEIKKLALDRMRLSTALKKNLEKISRQSSVLMDNMKHLLELNKLIMKSQQESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKNKQKIDLDSMENSERIKIIRQNLQMEIKITTVIQHVFQNLILGSKVNWAEDPALKEIVLQLEKNVDMM

Target ORF information:

RefSeq Version	NM_022909.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein H (CENPH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022909.3

ORF Insert Sequence:

ATGGAGGAGC AGCCCCAGAT GCAAGACGCC GACGAGCCCG CGGACTCCGG AGGGGAAGGC 
CGGGCAGGCG GGCCACCGCA GGTCGCCGGC GCCCAGGCGG CGTGCAGCGA GGACCGCATG 
ACCCTGCTCC TCAGGCTGAG AGCACAGACA AAACAACAAC TCTTAGAATA TAAATCAATG 
GTTGATGCAA GTGAAGAAAA AACTCCAGAA CAAATTATGC AAGAAAAGCA AATCGAAGCT 
AAAATTGAAG ACCTGGAAAA TGAAATTGAA GAGGTAAAAG TTGCTTTTGA GATAAAAAAG 
CTTGCATTAG ACAGGATGAG ACTTTCAACT GCACTTAAAA AAAACCTGGA GAAAATTAGC 
AGACAGTCTA GTGTGCTCAT GGATAACATG AAACACCTAT TAGAGCTAAA TAAATTAATA 
ATGAAATCAC AGCAGGAATC TTGGGATTTA GAGGAAAAAC TGCTTGATAT TAGAAAGAAG 
AGATTGCAAT TAAAACAAGC TTCAGAAAGT AAGCTTTTAG AAATACAGAC TGAAAAGAAC 
AAACAGAAGA TTGATTTGGA CAGTATGGAA AACTCAGAGA GGATAAAGAT CATACGACAA 
AACCTACAGA TGGAGATAAA AATTACTACT GTTATTCAAC ATGTGTTCCA GAACCTTATT 
TTGGGGAGTA AAGTCAATTG GGCAGAGGAT CCTGCCCTTA AGGAAATTGT TCTGCAGCTT 
GAGAAGAATG TTGACATGAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CENP-H regulates the cell growth of human hepatocellular carcinoma cells through the mitochondrial apoptotic pathway.
Oncology reports37(6)3484-3492(2017 Jun)
Lu G,Hou H,Lu X,Ke X,Wang X,Zhang D,Zhao Y,Zhang J,Ren M,He S

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis.
The Journal of biological chemistry290(45)27053-66(2015 Nov)
Zhu L,Wang Z,Wang W,Wang C,Hua S,Su Z,Brako L,Garcia-Barrio M,Ye M,Wei X,Zou H,Ding X,Liu L,Liu X,Yao X

Upregulation of centromere protein H is associated with progression of renal cell carcinoma.
Journal of molecular histology46(4-5)377-85(2015 Oct)
Wu X,Lin Y,Shi L,Huang Y,Lai C,Wang Y,Zhang M,Wang S,Heng B,Yu G,Du X,Fang L,Fu Y,Chen J,Guo Z,Su Z,Wu S

Overexpression of CENP-H as a novel prognostic biomarker for human hepatocellular carcinoma progression and patient survival.
Oncology reports30(5)2238-44(2013 Nov)
Lu G,Shan T,He S,Ren M,Zhu M,Hu Y,Lu X,Zhang D

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CENPH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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