RBM15 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RBM15
Entrez Gene ID	64783
Full Name	RNA binding motif protein 15
Synonyms	OTT,OTT1,SPEN
General protein information	Preferred Names RNA binding motif protein 15 Names putative RNA-binding protein 15 one twenty two protein one twenty-two one-twenty two protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p13.3
Summary	Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM, Feb 2009].
Disorder MIM:	606077
Disorder Html:	Megakaryoblastic leukemia, acute (2)

mRNA and Protein(s)

mRNA	Protein	Name
NM_022768.4	NP_073605.4	RNA-binding protein 15 isoform 1
NM_001201545.1	NP_001188474.1	RNA-binding protein 15 isoform 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rbm15	XP_003749428.1
Gallus gallus (chicken)	LOC100859595	XP_003642784.1
Homo sapiens (human)	RBM15	NP_073605.4
Pan troglodytes (chimpanzee)	RBM15	XP_524800.2
Canis lupus familiaris (dog)	RBM15	XP_537035.1
Mus musculus (house mouse)	Rbm15	NP_001039272.1
Xenopus tropicalis (tropical clawed frog)	rbm15	XP_002933033.1
Bos taurus (cattle)	RBM15	XP_005204276.1
Danio rerio (zebrafish)	rbm15	XP_001335820.1
	RBM15	XP_001100087.1

Gene Ontology
Bibliography

Related articles in PubMed

MED12, TERT promoter and RBM15 mutations in primary and recurrent phyllodes tumours.
Garcia-Dios DA, Levi D, Shah V, Gillett C, Simpson MA, Hanby A, Tomlinson I, Sawyer EJ
British journal of cancer(2018 Jan)

m(6)A RNA methylation promotes XIST-mediated transcriptional repression.
Patil DP, Chen CK, Pickering BF, Chow A, Jackson C, Guttman M, Jaffrey SR
Nature537(7620)369-373(2016 09)

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.
Zhang L, Tran NT, Su H, Wang R, Lu Y, Tang H, Aoyagi S, Guo A, Khodadadi-Jamayran A, Zhou D, Qian K, Hricik T, C?t? J, Han X, Zhou W, Laha S, Abdel-Wahab O, Levine RL, Raffel G, Liu Y, Chen D, Li H, Townes T, Wang H, Deng H, Zheng YG, Leslie C, Luo M, Zhao X
eLife4(2015 Nov)

Ott1 (Rbm15) regulates thrombopoietin response in hematopoietic stem cells through alternative splicing of c-Mpl.
Xiao N, Laha S, Das SP, Morlock K, Jesneck JL, Raffel GD
Blood125(6)941-8(2015 Feb)

Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle.
Horiuchi K, Kawamura T, Iwanari H, Ohashi R, Naito M, Kodama T, Hamakubo T
The Journal of biological chemistry288(46)33292-302(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors demonstrate that RBM15 is methylated by protein arginine methyltransferase 1 (PRMT1) at residue R578, leading to its degradation via ubiquitylation by an E3 ligase (CNOT4).
Title: Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.

OTT1 regulates the alternative splicing of Mpl-TR, a truncated isoform of c-Mpl, which modulates Thrombopoietin-mediated signaling.
Title: Ott1 (Rbm15) regulates thrombopoietin response in hematopoietic stem cells through alternative splicing of c-Mpl.

RBM15 does indeed play a critical role in the survival of chronic myelogenous leukemia (CML) cells, which may have potential application in designing molecular therapies for CML treatment.
Title: Biological effects of decreasing RBM15 on chronic myelogenous leukemia cells.

both Rbm15 and the leukemogenic Rbm15-Mkl1 fusion protein interact with the Setd1b histone H3-Lys4 methyltransferase
Title: Rbm15-Mkl1 interacts with the Setd1b histone H3-Lys4 methyltransferase via a SPOC domain that is required for cytokine-independent proliferation.

A four-way variant translocation originating the RBM15-MKL1 fusion gene is associated with acute megakaryoblastic leukemia.
Title: Acute megakaryoblastic leukemia with a four-way variant translocation originating the RBM15-MKL1 fusion gene.

The following RBM15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RBM15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu55649	NM_001201545.1 Latest version!	Homo sapiens RNA binding motif protein 15 (RBM15), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu03916	NM_022768.4 Latest version!	Homo sapiens RNA binding motif protein 15 (RBM15), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$657.30 $939.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RBM15 ( NM_001201545.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188474.1 Homo sapiens RNA binding motif protein 15 (RBM15), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55649
Clone ID Related Accession (Same CDS sequence)	NM_001201545.1
Accession Version	NM_001201545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	RNA-binding protein 15 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF368062.1, BC098140.1, BC006397.2 and AI863760.1. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF368062.1, BC047479.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGAGGACTG CGGGGCGGGA CCCTGTGCCG CGGCGGAGTC CAAGATGGCG GCGTGCGGTT 
CCGCTGTGTG AAACGAGCGC GGGGCGGCGG GTTACTCAGC TCCGCGGAGA CGACCTCCGA 
CGACCCGCAA CAATGAAGGG AAAAGAGCGC TCGCCAGTGA AGGCCAAACG CTCCCGTGGT 
GGTGAGGACT CGACTTCCCG CGGTGAGCGG AGCAAGAAGT TAGGGGGCTC TGGTGGCAGC 
AATGGGAGCA GCAGCGGAAA GACCGATAGC GGCGGTGGGT CGCGGCGGAG TCTCCACCTG 
GACAAGTCCA GCAGTCGAGG TGGCAGCCGC GAGTATGATA CCGGTGGGGG CAGCTCCAGT 
AGCCGCTTGC ATAGTTATAG CTCCCCGAGC ACCAAAAATT CTTCGGGCGG GGGCGAGTCG 
CGCAGCAGCT CCCGGGGTGG AGGCGGGGAG TCACGTTCCT CTGGGGCCGC CTCCTCAGCT 
CCCGGCGGCG GGGACGGCGC GGAATACAAG ACTCTGAAGA TAAGCGAGTT GGGGTCCCAG 
CTTAGTGACG AAGCGGTGGA GGACGGCCTG TTTCATGAGT TCAAACGCTT CGGTGATGTA 
AGTGTGAAAA TCAGTCATCT GTCGGGTTCT GGCAGCGGGG ATGAGCGGGT AGCCTTTGTG 
AACTTCCGGC GGCCAGAGGA CGCGCGGGCG GCCAAGCATG CCAGAGGCCG CCTGGTGCTC 
TATGACCGGC CTCTGAAGAT AGAAGCTGTG TATGTGAGCC GGCGCCGCAG CCGCTCCCCT 
TTAGACAAAG ATACTTATCC TCCATCAGCC AGTGTGGTCG GGGCCTCTGT AGGTGGTCAC 
CGGCACCCCC CTGGAGGTGG TGGAGGCCAG AGATCACTTT CCCCTGGTGG CGCTGCTTTG 
GGATACAGAG ACTACCGGCT GCAGCAGTTG GCTCTTGGCC GCCTGCCCCC TCCACCTCCG 
CCACCATTGC CTCGAGACCT GGAGAGAGAA AGAGACTACC CGTTCTATGA GAGAGTGCGC 
CCTGCATACA GTCTTGAGCC AAGGGTGGGA GCTGGAGCAG GTGCTGCTCC TTTCAGAGAA 
GTGGATGAGA TTTCACCCGA GGATGATCAG CGAGCTAACC GGACGCTCTT CTTGGGCAAC 
CTAGACATCA CTGTAACGGA GAGTGATTTA AGAAGGGCGT TTGATCGCTT TGGAGTCATC 
ACAGAAGTAG ATATCAAGAG GCCTTCTCGC GGCCAGACTA GTACTTACGG CTTTCTCAAA 
TTTGAGAACT TAGATATGTC TCACCGGGCC AAATTAGCAA TGTCTGGCAA AATTATAATT 
CGGAATCCTA TCAAAATTGG TTATGGTAAA GCTACACCCA CCACCCGCCT CTGGGTGGGA 
GGCCTGGGAC CTTGGGTTCC TCTTGCTGCC CTGGCACGAG AATTTGATCG ATTTGGCACC 
ATACGCACCA TAGACTACCG AAAAGGTGAT AGTTGGGCAT ATATCCAGTA TGAAAGCCTG 
GATGCAGCGC ATGCTGCCTG GACCCATATG CGGGGCTTCC CACTTGGTGG CCCAGATCGA 
CGCCTTAGAG TAGACTTTGC CGACACCGAA CATCGTTACC AGCAGCAGTA TCTGCAGCCT 
CTGCCCTTGA CTCATTATGA GCTGGTGACA GATGCTTTTG GACATCGGGC ACCAGACCCT 
TTGAGGGGTG CTCGGGATAG GACACCACCC TTACTATACA GAGATCGTGA TAGGGACCTT 
TATCCTGACT CTGATTGGGT GCCACCCCCA CCCCCAGTCC GAGAACGCAG CACTCGGACT 
GCAGCTACTT CTGTGCCTGC TTACGAGCCA CTGGATAGCC TAGATCGCAG GCGGGATGGT 
TGGTCCTTGG ACCGGGACAG AGGTGATCGA GATCTGCCCA GCAGCAGAGA CCAGCCTAGG 
AAGCGAAGGC TGCCTGAGGA GAGTGGAGGA CGTCATCTGG ATAGGTCTCC TGAGAGTGAC 
CGCCCACGAA AACGTCACTG CGCTCCTTCT CCTGACCGCA GTCCAGAATT GAGCAGTAGC 
CGGGATCGTT ACAACAGCGA CAATGATCGA TCTTCCCGTC TTCTCTTGGA AAGGCCCTCT 
CCAATCAGAG ACAGACGAGG TAGTTTGGAG AAGAGCCAGG GTGACAAGCG AGACCGTAAA 
AACTCTGCAT CAGCTGAACG AGATAGGAAG CACCGGACAA CTGCTCCCAC TGAGGGAAAA 
AGCCCTCTGA AAAAAGAAGA CCGCTCTGAT GGGAGTGCAC CTAGCACCAG CACTGCTTCC 
TCCAAGCTGA AGTCCCCGTC CCAGAAACAG GATGGGGGGA CAGCCCCTGT GGCATCAGCC 
TCTCCCAAAC TCTGTTTGGC CTGGCAGGGC ATGCTTCTAC TGAAGAACAG CAACTTTCCT 
TCCAACATGC ATCTGTTGCA GGGTGACCTC CAAGTGGCTA GTAGTCTTCT TGTGGAGGGT 
TCAACTGGAG GCAAAGTGGC CCAGCTCAAG ATCACTCAGC GTCTCCGTTT GGACCAGCCC 
AAGTTGGATG AAGTAACTCG ACGCATCAAA GTAGCAGGGC CCAATGGTTA TGCCATTCTT 
TTGGCTGTGC CTGGAAGTTC TGACAGCCGG TCCTCCTCTT CCTCAGCTGC ATCAGACACT 
GCCACTTCTA CTCAGAGGCC ACTTAGGAAC CTTGTGTCCT ATTTAAAGCA AAAGCAGGCA 
GCCGGGGTGA TCAGCCTCCC TGTGGGGGGC AACAAAGACA AGGAAAACAC CGGGGTCCTT 
CATGCCTTCC CACCTTGTGA GTTCTCCCAG CAGTTCCTGG ATTCCCCTGC CAAGGCACTG 
GCCAAATCTG AAGAAGATTA CCTGGTCATG ATCATTGTCC GTGCAAAACT GGTTGAACAG 
CGGATGAAGA TATGGAATTC AAAGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188474.1
CDS	84..2993
Translation	MRTAGRDPVPRRSPRWRRAVPLCETSAGRRVTQLRGDDLRRPATMKGKERSPVKAKRSRGGEDSTSRGERSKKLGGSGGSNGSSSGKTDSGGGSRRSLHLDKSSSRGGSREYDTGGGSSSSRLHSYSSPSTKNSSGGGESRSSSRGGGGESRSSGAASSAPGGGDGAEYKTLKISELGSQLSDEAVEDGLFHEFKRFGDVSVKISHLSGSGSGDERVAFVNFRRPEDARAAKHARGRLVLYDRPLKIEAVYVSRRRSRSPLDKDTYPPSASVVGASVGGHRHPPGGGGGQRSLSPGGAALGYRDYRLQQLALGRLPPPPPPPLPRDLERERDYPFYERVRPAYSLEPRVGAGAGAAPFREVDEISPEDDQRANRTLFLGNLDITVTESDLRRAFDRFGVITEVDIKRPSRGQTSTYGFLKFENLDMSHRAKLAMSGKIIIRNPIKIGYGKATPTTRLWVGGLGPWVPLAALAREFDRFGTIRTIDYRKGDSWAYIQYESLDAAHAAWTHMRGFPLGGPDRRLRVDFADTEHRYQQQYLQPLPLTHYELVTDAFGHRAPDPLRGARDRTPPLLYRDRDRDLYPDSDWVPPPPPVRERSTRTAATSVPAYEPLDSLDRRRDGWSLDRDRGDRDLPSSRDQPRKRRLPEESGGRHLDRSPESDRPRKRHCAPSPDRSPELSSSRDRYNSDNDRSSRLLLERPSPIRDRRGSLEKSQGDKRDRKNSASAERDRKHRTTAPTEGKSPLKKEDRSDGSAPSTSTASSKLKSPSQKQDGGTAPVASASPKLCLAWQGMLLLKNSNFPSNMHLLQGDLQVASSLLVEGSTGGKVAQLKITQRLRLDQPKLDEVTRRIKVAGPNGYAILLAVPGSSDSRSSSSSAASDTATSTQRPLRNLVSYLKQKQAAGVISLPVGGNKDKENTGVLHAFPPCEFSQQFLDSPAKALAKSEEDYLVMIIVRAKLVEQRMKIWNSKL

Target ORF information:

RefSeq Version	NM_001201545.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA binding motif protein 15 (RBM15), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201545.1

ORF Insert Sequence:

ATGAGGACTG CGGGGCGGGA CCCTGTGCCG CGGCGGAGTC CAAGATGGCG GCGTGCGGTT 
CCGCTGTGTG AAACGAGCGC GGGGCGGCGG GTTACTCAGC TCCGCGGAGA CGACCTCCGA 
CGACCCGCAA CAATGAAGGG AAAAGAGCGC TCGCCAGTGA AGGCCAAACG CTCCCGTGGT 
GGTGAGGACT CGACTTCCCG CGGTGAGCGG AGCAAGAAGT TAGGGGGCTC TGGTGGCAGC 
AATGGGAGCA GCAGCGGAAA GACCGATAGC GGCGGTGGGT CGCGGCGGAG TCTCCACCTG 
GACAAGTCCA GCAGTCGAGG TGGCAGCCGC GAGTATGATA CCGGTGGGGG CAGCTCCAGT 
AGCCGCTTGC ATAGTTATAG CTCCCCGAGC ACCAAAAATT CTTCGGGCGG GGGCGAGTCG 
CGCAGCAGCT CCCGGGGTGG AGGCGGGGAG TCACGTTCCT CTGGGGCCGC CTCCTCAGCT 
CCCGGCGGCG GGGACGGCGC GGAATACAAG ACTCTGAAGA TAAGCGAGTT GGGGTCCCAG 
CTTAGTGACG AAGCGGTGGA GGACGGCCTG TTTCATGAGT TCAAACGCTT CGGTGATGTA 
AGTGTGAAAA TCAGTCATCT GTCGGGTTCT GGCAGCGGGG ATGAGCGGGT AGCCTTTGTG 
AACTTCCGGC GGCCAGAGGA CGCGCGGGCG GCCAAGCATG CCAGAGGCCG CCTGGTGCTC 
TATGACCGGC CTCTGAAGAT AGAAGCTGTG TATGTGAGCC GGCGCCGCAG CCGCTCCCCT 
TTAGACAAAG ATACTTATCC TCCATCAGCC AGTGTGGTCG GGGCCTCTGT AGGTGGTCAC 
CGGCACCCCC CTGGAGGTGG TGGAGGCCAG AGATCACTTT CCCCTGGTGG CGCTGCTTTG 
GGATACAGAG ACTACCGGCT GCAGCAGTTG GCTCTTGGCC GCCTGCCCCC TCCACCTCCG 
CCACCATTGC CTCGAGACCT GGAGAGAGAA AGAGACTACC CGTTCTATGA GAGAGTGCGC 
CCTGCATACA GTCTTGAGCC AAGGGTGGGA GCTGGAGCAG GTGCTGCTCC TTTCAGAGAA 
GTGGATGAGA TTTCACCCGA GGATGATCAG CGAGCTAACC GGACGCTCTT CTTGGGCAAC 
CTAGACATCA CTGTAACGGA GAGTGATTTA AGAAGGGCGT TTGATCGCTT TGGAGTCATC 
ACAGAAGTAG ATATCAAGAG GCCTTCTCGC GGCCAGACTA GTACTTACGG CTTTCTCAAA 
TTTGAGAACT TAGATATGTC TCACCGGGCC AAATTAGCAA TGTCTGGCAA AATTATAATT 
CGGAATCCTA TCAAAATTGG TTATGGTAAA GCTACACCCA CCACCCGCCT CTGGGTGGGA 
GGCCTGGGAC CTTGGGTTCC TCTTGCTGCC CTGGCACGAG AATTTGATCG ATTTGGCACC 
ATACGCACCA TAGACTACCG AAAAGGTGAT AGTTGGGCAT ATATCCAGTA TGAAAGCCTG 
GATGCAGCGC ATGCTGCCTG GACCCATATG CGGGGCTTCC CACTTGGTGG CCCAGATCGA 
CGCCTTAGAG TAGACTTTGC CGACACCGAA CATCGTTACC AGCAGCAGTA TCTGCAGCCT 
CTGCCCTTGA CTCATTATGA GCTGGTGACA GATGCTTTTG GACATCGGGC ACCAGACCCT 
TTGAGGGGTG CTCGGGATAG GACACCACCC TTACTATACA GAGATCGTGA TAGGGACCTT 
TATCCTGACT CTGATTGGGT GCCACCCCCA CCCCCAGTCC GAGAACGCAG CACTCGGACT 
GCAGCTACTT CTGTGCCTGC TTACGAGCCA CTGGATAGCC TAGATCGCAG GCGGGATGGT 
TGGTCCTTGG ACCGGGACAG AGGTGATCGA GATCTGCCCA GCAGCAGAGA CCAGCCTAGG 
AAGCGAAGGC TGCCTGAGGA GAGTGGAGGA CGTCATCTGG ATAGGTCTCC TGAGAGTGAC 
CGCCCACGAA AACGTCACTG CGCTCCTTCT CCTGACCGCA GTCCAGAATT GAGCAGTAGC 
CGGGATCGTT ACAACAGCGA CAATGATCGA TCTTCCCGTC TTCTCTTGGA AAGGCCCTCT 
CCAATCAGAG ACAGACGAGG TAGTTTGGAG AAGAGCCAGG GTGACAAGCG AGACCGTAAA 
AACTCTGCAT CAGCTGAACG AGATAGGAAG CACCGGACAA CTGCTCCCAC TGAGGGAAAA 
AGCCCTCTGA AAAAAGAAGA CCGCTCTGAT GGGAGTGCAC CTAGCACCAG CACTGCTTCC 
TCCAAGCTGA AGTCCCCGTC CCAGAAACAG GATGGGGGGA CAGCCCCTGT GGCATCAGCC 
TCTCCCAAAC TCTGTTTGGC CTGGCAGGGC ATGCTTCTAC TGAAGAACAG CAACTTTCCT 
TCCAACATGC ATCTGTTGCA GGGTGACCTC CAAGTGGCTA GTAGTCTTCT TGTGGAGGGT 
TCAACTGGAG GCAAAGTGGC CCAGCTCAAG ATCACTCAGC GTCTCCGTTT GGACCAGCCC 
AAGTTGGATG AAGTAACTCG ACGCATCAAA GTAGCAGGGC CCAATGGTTA TGCCATTCTT 
TTGGCTGTGC CTGGAAGTTC TGACAGCCGG TCCTCCTCTT CCTCAGCTGC ATCAGACACT 
GCCACTTCTA CTCAGAGGCC ACTTAGGAAC CTTGTGTCCT ATTTAAAGCA AAAGCAGGCA 
GCCGGGGTGA TCAGCCTCCC TGTGGGGGGC AACAAAGACA AGGAAAACAC CGGGGTCCTT 
CATGCCTTCC CACCTTGTGA GTTCTCCCAG CAGTTCCTGG ATTCCCCTGC CAAGGCACTG 
GCCAAATCTG AAGAAGATTA CCTGGTCATG ATCATTGTCC GTGCAAAACT GGTTGAACAG 
CGGATGAAGA TATGGAATTC AAAGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RBM15 ( NM_022768.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_073605.4 Homo sapiens RNA binding motif protein 15 (RBM15), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03916
Clone ID Related Accession (Same CDS sequence)	NM_022768.4
Accession Version	NM_022768.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-10
Organism	Homo sapiens(human)
Product	RNA-binding protein 15 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC042587.1, BC062316.1, BC047479.1 and AW173262.1. On Jun 2, 2004 this sequence version replaced NM_022768.3. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF368063.1, BC042587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGAGGACTG CGGGGCGGGA CCCTGTGCCG CGGCGGAGTC CAAGATGGCG GCGTGCGGTT 
CCGCTGTGTG AAACGAGCGC GGGGCGGCGG GTTACTCAGC TCCGCGGAGA CGACCTCCGA 
CGACCCGCAA CAATGAAGGG AAAAGAGCGC TCGCCAGTGA AGGCCAAACG CTCCCGTGGT 
GGTGAGGACT CGACTTCCCG CGGTGAGCGG AGCAAGAAGT TAGGGGGCTC TGGTGGCAGC 
AATGGGAGCA GCAGCGGAAA GACCGATAGC GGCGGTGGGT CGCGGCGGAG TCTCCACCTG 
GACAAGTCCA GCAGTCGAGG TGGCAGCCGC GAGTATGATA CCGGTGGGGG CAGCTCCAGT 
AGCCGCTTGC ATAGTTATAG CTCCCCGAGC ACCAAAAATT CTTCGGGCGG GGGCGAGTCG 
CGCAGCAGCT CCCGGGGTGG AGGCGGGGAG TCACGTTCCT CTGGGGCCGC CTCCTCAGCT 
CCCGGCGGCG GGGACGGCGC GGAATACAAG ACTCTGAAGA TAAGCGAGTT GGGGTCCCAG 
CTTAGTGACG AAGCGGTGGA GGACGGCCTG TTTCATGAGT TCAAACGCTT CGGTGATGTA 
AGTGTGAAAA TCAGTCATCT GTCGGGTTCT GGCAGCGGGG ATGAGCGGGT AGCCTTTGTG 
AACTTCCGGC GGCCAGAGGA CGCGCGGGCG GCCAAGCATG CCAGAGGCCG CCTGGTGCTC 
TATGACCGGC CTCTGAAGAT AGAAGCTGTG TATGTGAGCC GGCGCCGCAG CCGCTCCCCT 
TTAGACAAAG ATACTTATCC TCCATCAGCC AGTGTGGTCG GGGCCTCTGT AGGTGGTCAC 
CGGCACCCCC CTGGAGGTGG TGGAGGCCAG AGATCACTTT CCCCTGGTGG CGCTGCTTTG 
GGATACAGAG ACTACCGGCT GCAGCAGTTG GCTCTTGGCC GCCTGCCCCC TCCACCTCCG 
CCACCATTGC CTCGAGACCT GGAGAGAGAA AGAGACTACC CGTTCTATGA GAGAGTGCGC 
CCTGCATACA GTCTTGAGCC AAGGGTGGGA GCTGGAGCAG GTGCTGCTCC TTTCAGAGAA 
GTGGATGAGA TTTCACCCGA GGATGATCAG CGAGCTAACC GGACGCTCTT CTTGGGCAAC 
CTAGACATCA CTGTAACGGA GAGTGATTTA AGAAGGGCGT TTGATCGCTT TGGAGTCATC 
ACAGAAGTAG ATATCAAGAG GCCTTCTCGC GGCCAGACTA GTACTTACGG CTTTCTCAAA 
TTTGAGAACT TAGATATGTC TCACCGGGCC AAATTAGCAA TGTCTGGCAA AATTATAATT 
CGGAATCCTA TCAAAATTGG TTATGGTAAA GCTACACCCA CCACCCGCCT CTGGGTGGGA 
GGCCTGGGAC CTTGGGTTCC TCTTGCTGCC CTGGCACGAG AATTTGATCG ATTTGGCACC 
ATACGCACCA TAGACTACCG AAAAGGTGAT AGTTGGGCAT ATATCCAGTA TGAAAGCCTG 
GATGCAGCGC ATGCTGCCTG GACCCATATG CGGGGCTTCC CACTTGGTGG CCCAGATCGA 
CGCCTTAGAG TAGACTTTGC CGACACCGAA CATCGTTACC AGCAGCAGTA TCTGCAGCCT 
CTGCCCTTGA CTCATTATGA GCTGGTGACA GATGCTTTTG GACATCGGGC ACCAGACCCT 
TTGAGGGGTG CTCGGGATAG GACACCACCC TTACTATACA GAGATCGTGA TAGGGACCTT 
TATCCTGACT CTGATTGGGT GCCACCCCCA CCCCCAGTCC GAGAACGCAG CACTCGGACT 
GCAGCTACTT CTGTGCCTGC TTACGAGCCA CTGGATAGCC TAGATCGCAG GCGGGATGGT 
TGGTCCTTGG ACCGGGACAG AGGTGATCGA GATCTGCCCA GCAGCAGAGA CCAGCCTAGG 
AAGCGAAGGC TGCCTGAGGA GAGTGGAGGA CGTCATCTGG ATAGGTCTCC TGAGAGTGAC 
CGCCCACGAA AACGTCACTG CGCTCCTTCT CCTGACCGCA GTCCAGAATT GAGCAGTAGC 
CGGGATCGTT ACAACAGCGA CAATGATCGA TCTTCCCGTC TTCTCTTGGA AAGGCCCTCT 
CCAATCAGAG ACAGACGAGG TAGTTTGGAG AAGAGCCAGG GTGACAAGCG AGACCGTAAA 
AACTCTGCAT CAGCTGAACG AGATAGGAAG CACCGGACAA CTGCTCCCAC TGAGGGAAAA 
AGCCCTCTGA AAAAAGAAGA CCGCTCTGAT GGGAGTGCAC CTAGCACCAG CACTGCTTCC 
TCCAAGCTGA AGTCCCCGTC CCAGAAACAG GATGGGGGGA CAGCCCCTGT GGCATCAGCC 
TCTCCCAAAC TCTGTTTGGC CTGGCAGGGC ATGCTTCTAC TGAAGAACAG CAACTTTCCT 
TCCAACATGC ATCTGTTGCA GGGTGACCTC CAAGTGGCTA GTAGTCTTCT TGTGGAGGGT 
TCAACTGGAG GCAAAGTGGC CCAGCTCAAG ATCACTCAGC GTCTCCGTTT GGACCAGCCC 
AAGTTGGATG AAGTAACTCG ACGCATCAAA GTAGCAGGGC CCAATGGTTA TGCCATTCTT 
TTGGCTGTGC CTGGAAGTTC TGACAGCCGG TCCTCCTCTT CCTCAGCTGC ATCAGACACT 
GCCACTTCTA CTCAGAGGCC ACTTAGGAAC CTTGTGTCCT ATTTAAAGCA AAAGCAGGCA 
GCCGGGGTGA TCAGCCTCCC TGTGGGGGGC AACAAAGACA AGGAAAACAC CGGGGTCCTT 
CATGCCTTCC CACCTTGTGA GTTCTCCCAG CAGTTCCTGG ATTCCCCTGC CAAGGCACTG 
GCCAAATCTG AAGAAGATTA CCTGGTCATG ATCATTGTCC GTGGGTTTGG TTTTCAGATA 
GGAGTTAGGT ATGAGAACAA GAAGAGAGAA AACTTGGCGC TGACCCTGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073605.4
CDS	84..3017
Translation	MRTAGRDPVPRRSPRWRRAVPLCETSAGRRVTQLRGDDLRRPATMKGKERSPVKAKRSRGGEDSTSRGERSKKLGGSGGSNGSSSGKTDSGGGSRRSLHLDKSSSRGGSREYDTGGGSSSSRLHSYSSPSTKNSSGGGESRSSSRGGGGESRSSGAASSAPGGGDGAEYKTLKISELGSQLSDEAVEDGLFHEFKRFGDVSVKISHLSGSGSGDERVAFVNFRRPEDARAAKHARGRLVLYDRPLKIEAVYVSRRRSRSPLDKDTYPPSASVVGASVGGHRHPPGGGGGQRSLSPGGAALGYRDYRLQQLALGRLPPPPPPPLPRDLERERDYPFYERVRPAYSLEPRVGAGAGAAPFREVDEISPEDDQRANRTLFLGNLDITVTESDLRRAFDRFGVITEVDIKRPSRGQTSTYGFLKFENLDMSHRAKLAMSGKIIIRNPIKIGYGKATPTTRLWVGGLGPWVPLAALAREFDRFGTIRTIDYRKGDSWAYIQYESLDAAHAAWTHMRGFPLGGPDRRLRVDFADTEHRYQQQYLQPLPLTHYELVTDAFGHRAPDPLRGARDRTPPLLYRDRDRDLYPDSDWVPPPPPVRERSTRTAATSVPAYEPLDSLDRRRDGWSLDRDRGDRDLPSSRDQPRKRRLPEESGGRHLDRSPESDRPRKRHCAPSPDRSPELSSSRDRYNSDNDRSSRLLLERPSPIRDRRGSLEKSQGDKRDRKNSASAERDRKHRTTAPTEGKSPLKKEDRSDGSAPSTSTASSKLKSPSQKQDGGTAPVASASPKLCLAWQGMLLLKNSNFPSNMHLLQGDLQVASSLLVEGSTGGKVAQLKITQRLRLDQPKLDEVTRRIKVAGPNGYAILLAVPGSSDSRSSSSSAASDTATSTQRPLRNLVSYLKQKQAAGVISLPVGGNKDKENTGVLHAFPPCEFSQQFLDSPAKALAKSEEDYLVMIIVRGFGFQIGVRYENKKRENLALTLL

Target ORF information:

RefSeq Version	NM_022768.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA binding motif protein 15 (RBM15), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022768.4

ORF Insert Sequence:

ATGAGGACTG CGGGGCGGGA CCCTGTGCCG CGGCGGAGTC CAAGATGGCG GCGTGCGGTT 
CCGCTGTGTG AAACGAGCGC GGGGCGGCGG GTTACTCAGC TCCGCGGAGA CGACCTCCGA 
CGACCCGCAA CAATGAAGGG AAAAGAGCGC TCGCCAGTGA AGGCCAAACG CTCCCGTGGT 
GGTGAGGACT CGACTTCCCG CGGTGAGCGG AGCAAGAAGT TAGGGGGCTC TGGTGGCAGC 
AATGGGAGCA GCAGCGGAAA GACCGATAGC GGCGGTGGGT CGCGGCGGAG TCTCCACCTG 
GACAAGTCCA GCAGTCGAGG TGGCAGCCGC GAGTATGATA CCGGTGGGGG CAGCTCCAGT 
AGCCGCTTGC ATAGTTATAG CTCCCCGAGC ACCAAAAATT CTTCGGGCGG GGGCGAGTCG 
CGCAGCAGCT CCCGGGGTGG AGGCGGGGAG TCACGTTCCT CTGGGGCCGC CTCCTCAGCT 
CCCGGCGGCG GGGACGGCGC GGAATACAAG ACTCTGAAGA TAAGCGAGTT GGGGTCCCAG 
CTTAGTGACG AAGCGGTGGA GGACGGCCTG TTTCATGAGT TCAAACGCTT CGGTGATGTA 
AGTGTGAAAA TCAGTCATCT GTCGGGTTCT GGCAGCGGGG ATGAGCGGGT AGCCTTTGTG 
AACTTCCGGC GGCCAGAGGA CGCGCGGGCG GCCAAGCATG CCAGAGGCCG CCTGGTGCTC 
TATGACCGGC CTCTGAAGAT AGAAGCTGTG TATGTGAGCC GGCGCCGCAG CCGCTCCCCT 
TTAGACAAAG ATACTTATCC TCCATCAGCC AGTGTGGTCG GGGCCTCTGT AGGTGGTCAC 
CGGCACCCCC CTGGAGGTGG TGGAGGCCAG AGATCACTTT CCCCTGGTGG CGCTGCTTTG 
GGATACAGAG ACTACCGGCT GCAGCAGTTG GCTCTTGGCC GCCTGCCCCC TCCACCTCCG 
CCACCATTGC CTCGAGACCT GGAGAGAGAA AGAGACTACC CGTTCTATGA GAGAGTGCGC 
CCTGCATACA GTCTTGAGCC AAGGGTGGGA GCTGGAGCAG GTGCTGCTCC TTTCAGAGAA 
GTGGATGAGA TTTCACCCGA GGATGATCAG CGAGCTAACC GGACGCTCTT CTTGGGCAAC 
CTAGACATCA CTGTAACGGA GAGTGATTTA AGAAGGGCGT TTGATCGCTT TGGAGTCATC 
ACAGAAGTAG ATATCAAGAG GCCTTCTCGC GGCCAGACTA GTACTTACGG CTTTCTCAAA 
TTTGAGAACT TAGATATGTC TCACCGGGCC AAATTAGCAA TGTCTGGCAA AATTATAATT 
CGGAATCCTA TCAAAATTGG TTATGGTAAA GCTACACCCA CCACCCGCCT CTGGGTGGGA 
GGCCTGGGAC CTTGGGTTCC TCTTGCTGCC CTGGCACGAG AATTTGATCG ATTTGGCACC 
ATACGCACCA TAGACTACCG AAAAGGTGAT AGTTGGGCAT ATATCCAGTA TGAAAGCCTG 
GATGCAGCGC ATGCTGCCTG GACCCATATG CGGGGCTTCC CACTTGGTGG CCCAGATCGA 
CGCCTTAGAG TAGACTTTGC CGACACCGAA CATCGTTACC AGCAGCAGTA TCTGCAGCCT 
CTGCCCTTGA CTCATTATGA GCTGGTGACA GATGCTTTTG GACATCGGGC ACCAGACCCT 
TTGAGGGGTG CTCGGGATAG GACACCACCC TTACTATACA GAGATCGTGA TAGGGACCTT 
TATCCTGACT CTGATTGGGT GCCACCCCCA CCCCCAGTCC GAGAACGCAG CACTCGGACT 
GCAGCTACTT CTGTGCCTGC TTACGAGCCA CTGGATAGCC TAGATCGCAG GCGGGATGGT 
TGGTCCTTGG ACCGGGACAG AGGTGATCGA GATCTGCCCA GCAGCAGAGA CCAGCCTAGG 
AAGCGAAGGC TGCCTGAGGA GAGTGGAGGA CGTCATCTGG ATAGGTCTCC TGAGAGTGAC 
CGCCCACGAA AACGTCACTG CGCTCCTTCT CCTGACCGCA GTCCAGAATT GAGCAGTAGC 
CGGGATCGTT ACAACAGCGA CAATGATCGA TCTTCCCGTC TTCTCTTGGA AAGGCCCTCT 
CCAATCAGAG ACAGACGAGG TAGTTTGGAG AAGAGCCAGG GTGACAAGCG AGACCGTAAA 
AACTCTGCAT CAGCTGAACG AGATAGGAAG CACCGGACAA CTGCTCCCAC TGAGGGAAAA 
AGCCCTCTGA AAAAAGAAGA CCGCTCTGAT GGGAGTGCAC CTAGCACCAG CACTGCTTCC 
TCCAAGCTGA AGTCCCCGTC CCAGAAACAG GATGGGGGGA CAGCCCCTGT GGCATCAGCC 
TCTCCCAAAC TCTGTTTGGC CTGGCAGGGC ATGCTTCTAC TGAAGAACAG CAACTTTCCT 
TCCAACATGC ATCTGTTGCA GGGTGACCTC CAAGTGGCTA GTAGTCTTCT TGTGGAGGGT 
TCAACTGGAG GCAAAGTGGC CCAGCTCAAG ATCACTCAGC GTCTCCGTTT GGACCAGCCC 
AAGTTGGATG AAGTAACTCG ACGCATCAAA GTAGCAGGGC CCAATGGTTA TGCCATTCTT 
TTGGCTGTGC CTGGAAGTTC TGACAGCCGG TCCTCCTCTT CCTCAGCTGC ATCAGACACT 
GCCACTTCTA CTCAGAGGCC ACTTAGGAAC CTTGTGTCCT ATTTAAAGCA AAAGCAGGCA 
GCCGGGGTGA TCAGCCTCCC TGTGGGGGGC AACAAAGACA AGGAAAACAC CGGGGTCCTT 
CATGCCTTCC CACCTTGTGA GTTCTCCCAG CAGTTCCTGG ATTCCCCTGC CAAGGCACTG 
GCCAAATCTG AAGAAGATTA CCTGGTCATG ATCATTGTCC GTGGGTTTGG TTTTCAGATA 
GGAGTTAGGT ATGAGAACAA GAAGAGAGAA AACTTGGCGC TGACCCTGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MED12, TERT promoter and RBM15 mutations in primary and recurrent phyllodes tumours.
British journal of cancer(2018 Jan)
Garcia-Dios DA,Levi D,Shah V,Gillett C,Simpson MA,Hanby A,Tomlinson I,Sawyer EJ

m(6)A RNA methylation promotes XIST-mediated transcriptional repression.
Nature537(7620)369-373(2016 09)
Patil DP,Chen CK,Pickering BF,Chow A,Jackson C,Guttman M,Jaffrey SR

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.
eLife4(2015 Nov)
Zhang L,Tran NT,Su H,Wang R,Lu Y,Tang H,Aoyagi S,Guo A,Khodadadi-Jamayran A,Zhou D,Qian K,Hricik T,C?t? J,Han X,Zhou W,Laha S,Abdel-Wahab O,Levine RL,Raffel G,Liu Y,Chen D,Li H,Townes T,Wang H,Deng H,Zheng YG,Leslie C,Luo M,Zhao X

Ott1 (Rbm15) regulates thrombopoietin response in hematopoietic stem cells through alternative splicing of c-Mpl.
Blood125(6)941-8(2015 Feb)
Xiao N,Laha S,Das SP,Morlock K,Jesneck JL,Raffel GD

Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle.
The Journal of biological chemistry288(46)33292-302(2013 Nov)
Horiuchi K,Kawamura T,Iwanari H,Ohashi R,Naito M,Kodama T,Hamakubo T

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RBM15 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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