GAREM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAREM1
Entrez Gene ID	64762
Full Name	GRB2 associated regulator of MAPK1 subtype 1
Synonyms	C18orf11,FAM59A,GAREM,Gm944
General protein information	Preferred Names GRB2 associated regulator of MAPK1 subtype 1 Names GRB2-associated and regulator of MAPK protein 1 GRB2 associated regulator of MAPK1 1 GRB2 associated, regulator of MAPK1 GRB2-associated and regulator of MAPK1 Grb2-associated and regulator of Erk/MAPK family with sequence similarity 59, member A protein FAM59A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18q12.1
Summary	This gene encodes an adaptor protein which functions in the epidermal growth factor (EGF) receptor-mediated signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017025919.1	XP_016881408.1	GRB2-associated and regulator of MAPK protein 1 isoform X3
NM_001242409.1	NP_001229338.1	GRB2-associated and regulator of MAPK protein 1 isoform 1
NM_022751.2	NP_073588.1	GRB2-associated and regulator of MAPK protein 1 isoform 2
NM_001242409.2	NP_001229338.1	GRB2-associated and regulator of MAPK protein 1 isoform 1
NM_022751.3	NP_073588.1	GRB2-associated and regulator of MAPK protein 1 isoform 2
XM_024451236.1	XP_024307004.1	GRB2-associated and regulator of MAPK protein 1 isoform X2
XM_024451234.1	XP_024307002.1	GRB2-associated and regulator of MAPK protein 1 isoform X1
XM_024451235.1	XP_024307003.1	GRB2-associated and regulator of MAPK protein 1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Garem	NP_001028617.2
Xenopus tropicalis (tropical clawed frog)	garem	XP_004915258.1
Homo sapiens (human)	GAREM	NP_001229338.1
Pan troglodytes (chimpanzee)	FAM59A	XP_524002.3
Canis lupus familiaris (dog)	FAM59A	XP_005623042.1
Rattus norvegicus (Norway rat)	Garem	XP_006254547.1
Gallus gallus (chicken)	FAM59A	XP_419182.2
Danio rerio (zebrafish)	garem	NP_956463.1
Bos taurus (cattle)	GAREM	XP_005224117.1

Gene Ontology
Bibliography

Related articles in PubMed

GAREM1 regulates the PR interval on electrocardiograms.
Kim HO, Lim JE, Kim MJ, Kang JO, Kim SM, Nam JM, Tak J, Konishi H, Nishino T, Koh IS, Jin YH, Baik HH, Kim JB, Kim MK, Choi BY, Lee SH, Jang Y, Shin J, Oh B
Journal of human genetics(2017 Dec)

Functional relationship between CABIT, SAM and 14-3-3 binding domains of GAREM1 that play a role in its subcellular localization.
Nishino T, Matsunaga R, Konishi H
Biochemical and biophysical research communications464(2)616-21(2015 Aug)

Phospho-tyrosine dependent protein-protein interaction network.
Grossmann A, Benlasfer N, Birth P, Hegele A, Wachsmuth F, Apelt L, Stelzl U
Molecular systems biology11(3)794(2015 Mar)

Identification of three novel genetic variations associated with electrocardiographic traits (QRS duration and PR interval) in East Asians.
Hong KW, Lim JE, Kim JW, Tabara Y, Ueshima H, Miki T, Matsuda F, Cho YS, Kim Y, Oh B
Human molecular genetics23(24)6659-67(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

suggest that the interplay between 14-3-3, SAM domain and CABIT domain might be responsible for the distribution of GAREM1 in mammalian cells.
Title: Functional relationship between CABIT, SAM and 14-3-3 binding domains of GAREM1 that play a role in its subcellular localization.

Data indicate that a subtype of GAREM, GAREM2, is specifically expressed in the mouse, rat, and human brain.
Title: A brain-specific Grb2-associated regulator of extracellular signal-regulated kinase (Erk)/mitogen-activated protein kinase (MAPK) (GAREM) subtype, GAREM2, contributes to neurite outgrowth of neuroblastoma cells by regulating Erk signaling.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association. (HuGE Navigator)
Title: Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.

Extracellular signal-regulated kinase activation in response to epidermal growth factor stimulation is regulated by the expression of GAREM in COS-7 and HeLa cells.
Title: GAREM, a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through the activation of extracellular signal-regulated kinase signaling.

The following GAREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21694	NM_001242409.2 Latest version!	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu00218	NM_022751.3 Latest version!	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu71459	XM_017025919.1 Latest version!	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu104569	XM_024451235.1 Latest version!	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu104569	XM_024451234.1 Latest version!	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu104570	XM_024451236.1 Latest version!	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu21694	NM_001242409.1	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu00218	NM_022751.2	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAREM1 ( NM_001242409.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229338.1 Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21694
Clone ID Related Accession (Same CDS sequence)	NM_001242409.1 , NM_001242409.2
Accession Version	NM_001242409.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	GRB2-associated and regulator of MAPK protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK025263.1, BC121068.1, AK025660.1, AC015563.11 and AI479499.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.258763.1, SRR1803617.223086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467150 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCAGCGTC 
ACTAAAACTG ACACAAATCC TTCTGAAAGC ACTCCTGTTT CCTGCTATCC ATGTAACCGA 
GTGAAAACTG ATTCTGTGGA CCTGAAATCC CCGTTTGGAA GTCCTTCTGC TGAAGCTGTG 
TCCTCTCGGC TCTCATGGCC TAACCATTAT TCAGGAGCAT CAGAAAGCCA GACCAGGAGT 
GACTTCCTGC TGGATCCAAG CAGGAGTTAT AGTTACCCTA GACAAAAGAC GCCAGGCACA 
CCAAAGAGAA ACTGCCCAGC ACCTTTTGAT TTTGATGGCT GTGAGCTCCT GGCCAGCCCC 
ACTAGCCCAG TCACTGCAGA ATTCAGTAGC AGCGTCTCTG GTTGTCCCAA GTCAGCCAGC 
TACTCTCTGG AGAGCACAGA TGTGAAATCT CTTGCAGCTG GTGTGACAAA GCAGAGTACG 
TCATGCCCTG CCTTACCCCC CAGGGCTCCA AAACTAGTGG AAGAGAAGGT CGCCTCCGAA 
ACATCTCCTT TGCCTCTGAA AATTGATGGT GCTGAGGAAG ACCCCAAGTC TGGGTCACCA 
GATCTCTCGG AGGACCAGTA TTTTGTTAAA AAGGGCATGC AGGACATCTT CTCTGCCTCC 
TACCCTTTCT CATCTCCGCT CCATCTCCAG CTGGCCCCCA GATCCTGTGG CGACGGTTCC 
CCATGGCAGC CACCTGCTGA CCTATCAGGA CTCTCTATAG AGGAAGTGTC CAAGTCACTA 
CGGTTCATTG GTTTGTCCGA AGATGTCATA TCATTCTTTG TTACTGAAAA GATTGATGGG 
AACCTGCTTG TTCAGCTAAC GGAAGAAATC CTCTCAGAGG ATTTCAAATT GAGCAAATTG 
CAGGTGAAGA AGATAATGCA ATTCATTAAT GGCTGGAGGC CCAAAATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229338.1
CDS	57..2687
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDNGECVEGLRENDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGESWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEECKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAVKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNISVTKTDTNPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRLSWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTSPVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASETSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGDGSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKIMQFINGWRPKI

Target ORF information:

RefSeq Version	NM_001242409.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242409.1

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCAGCGTC 
ACTAAAACTG ACACAAATCC TTCTGAAAGC ACTCCTGTTT CCTGCTATCC ATGTAACCGA 
GTGAAAACTG ATTCTGTGGA CCTGAAATCC CCGTTTGGAA GTCCTTCTGC TGAAGCTGTG 
TCCTCTCGGC TCTCATGGCC TAACCATTAT TCAGGAGCAT CAGAAAGCCA GACCAGGAGT 
GACTTCCTGC TGGATCCAAG CAGGAGTTAT AGTTACCCTA GACAAAAGAC GCCAGGCACA 
CCAAAGAGAA ACTGCCCAGC ACCTTTTGAT TTTGATGGCT GTGAGCTCCT GGCCAGCCCC 
ACTAGCCCAG TCACTGCAGA ATTCAGTAGC AGCGTCTCTG GTTGTCCCAA GTCAGCCAGC 
TACTCTCTGG AGAGCACAGA TGTGAAATCT CTTGCAGCTG GTGTGACAAA GCAGAGTACG 
TCATGCCCTG CCTTACCCCC CAGGGCTCCA AAACTAGTGG AAGAGAAGGT CGCCTCCGAA 
ACATCTCCTT TGCCTCTGAA AATTGATGGT GCTGAGGAAG ACCCCAAGTC TGGGTCACCA 
GATCTCTCGG AGGACCAGTA TTTTGTTAAA AAGGGCATGC AGGACATCTT CTCTGCCTCC 
TACCCTTTCT CATCTCCGCT CCATCTCCAG CTGGCCCCCA GATCCTGTGG CGACGGTTCC 
CCATGGCAGC CACCTGCTGA CCTATCAGGA CTCTCTATAG AGGAAGTGTC CAAGTCACTA 
CGGTTCATTG GTTTGTCCGA AGATGTCATA TCATTCTTTG TTACTGAAAA GATTGATGGG 
AACCTGCTTG TTCAGCTAAC GGAAGAAATC CTCTCAGAGG ATTTCAAATT GAGCAAATTG 
CAGGTGAAGA AGATAATGCA ATTCATTAAT GGCTGGAGGC CCAAAATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( NM_001242409.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229338.1 Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21694
Clone ID Related Accession (Same CDS sequence)	NM_001242409.1 , NM_001242409.2
Accession Version	NM_001242409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	GRB2-associated and regulator of MAPK protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009835.14, AK025263.1, BC121068.1, AK025660.1, AC015563.11 and AI479499.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001242409.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.258763.1, SRR1803617.223086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467150 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000269209.7/ ENSP00000269209.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCAGCGTC 
ACTAAAACTG ACACAAATCC TTCTGAAAGC ACTCCTGTTT CCTGCTATCC ATGTAACCGA 
GTGAAAACTG ATTCTGTGGA CCTGAAATCC CCGTTTGGAA GTCCTTCTGC TGAAGCTGTG 
TCCTCTCGGC TCTCATGGCC TAACCATTAT TCAGGAGCAT CAGAAAGCCA GACCAGGAGT 
GACTTCCTGC TGGATCCAAG CAGGAGTTAT AGTTACCCTA GACAAAAGAC GCCAGGCACA 
CCAAAGAGAA ACTGCCCAGC ACCTTTTGAT TTTGATGGCT GTGAGCTCCT GGCCAGCCCC 
ACTAGCCCAG TCACTGCAGA ATTCAGTAGC AGCGTCTCTG GTTGTCCCAA GTCAGCCAGC 
TACTCTCTGG AGAGCACAGA TGTGAAATCT CTTGCAGCTG GTGTGACAAA GCAGAGTACG 
TCATGCCCTG CCTTACCCCC CAGGGCTCCA AAACTAGTGG AAGAGAAGGT CGCCTCCGAA 
ACATCTCCTT TGCCTCTGAA AATTGATGGT GCTGAGGAAG ACCCCAAGTC TGGGTCACCA 
GATCTCTCGG AGGACCAGTA TTTTGTTAAA AAGGGCATGC AGGACATCTT CTCTGCCTCC 
TACCCTTTCT CATCTCCGCT CCATCTCCAG CTGGCCCCCA GATCCTGTGG CGACGGTTCC 
CCATGGCAGC CACCTGCTGA CCTATCAGGA CTCTCTATAG AGGAAGTGTC CAAGTCACTA 
CGGTTCATTG GTTTGTCCGA AGATGTCATA TCATTCTTTG TTACTGAAAA GATTGATGGG 
AACCTGCTTG TTCAGCTAAC GGAAGAAATC CTCTCAGAGG ATTTCAAATT GAGCAAATTG 
CAGGTGAAGA AGATAATGCA ATTCATTAAT GGCTGGAGGC CCAAAATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229338.1
CDS	455..3085
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDNGECVEGLRENDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGESWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEECKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAVKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNISVTKTDTNPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRLSWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTSPVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASETSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGDGSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKIMQFINGWRPKI

Target ORF information:

RefSeq Version	NM_001242409.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242409.2

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCAGCGTC 
ACTAAAACTG ACACAAATCC TTCTGAAAGC ACTCCTGTTT CCTGCTATCC ATGTAACCGA 
GTGAAAACTG ATTCTGTGGA CCTGAAATCC CCGTTTGGAA GTCCTTCTGC TGAAGCTGTG 
TCCTCTCGGC TCTCATGGCC TAACCATTAT TCAGGAGCAT CAGAAAGCCA GACCAGGAGT 
GACTTCCTGC TGGATCCAAG CAGGAGTTAT AGTTACCCTA GACAAAAGAC GCCAGGCACA 
CCAAAGAGAA ACTGCCCAGC ACCTTTTGAT TTTGATGGCT GTGAGCTCCT GGCCAGCCCC 
ACTAGCCCAG TCACTGCAGA ATTCAGTAGC AGCGTCTCTG GTTGTCCCAA GTCAGCCAGC 
TACTCTCTGG AGAGCACAGA TGTGAAATCT CTTGCAGCTG GTGTGACAAA GCAGAGTACG 
TCATGCCCTG CCTTACCCCC CAGGGCTCCA AAACTAGTGG AAGAGAAGGT CGCCTCCGAA 
ACATCTCCTT TGCCTCTGAA AATTGATGGT GCTGAGGAAG ACCCCAAGTC TGGGTCACCA 
GATCTCTCGG AGGACCAGTA TTTTGTTAAA AAGGGCATGC AGGACATCTT CTCTGCCTCC 
TACCCTTTCT CATCTCCGCT CCATCTCCAG CTGGCCCCCA GATCCTGTGG CGACGGTTCC 
CCATGGCAGC CACCTGCTGA CCTATCAGGA CTCTCTATAG AGGAAGTGTC CAAGTCACTA 
CGGTTCATTG GTTTGTCCGA AGATGTCATA TCATTCTTTG TTACTGAAAA GATTGATGGG 
AACCTGCTTG TTCAGCTAAC GGAAGAAATC CTCTCAGAGG ATTTCAAATT GAGCAAATTG 
CAGGTGAAGA AGATAATGCA ATTCATTAAT GGCTGGAGGC CCAAAATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( NM_022751.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_073588.1 Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu00218
Clone ID Related Accession (Same CDS sequence)	NM_022751.2 , NM_022751.3
Accession Version	NM_022751.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	GRB2-associated and regulator of MAPK protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK025263.1, AK025660.1, AC015563.11 and AI479499.1. On Jun 3, 2011 this sequence version replaced NM_022751.1. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at the 3' terminal exon compared to variant 1. This results in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK025263.1, SRR1803617.91166.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCGTCACT 
AAAACTGACA CAAATCCTTC TGAAAGCACT CCTGTTTCCT GCTATCCATG TAACCGAGTG 
AAAACTGATT CTGTGGACCT GAAATCCCCG TTTGGAAGTC CTTCTGCTGA AGCTGTGTCC 
TCTCGGCTCT CATGGCCTAA CCATTATTCA GGAGCATCAG AAAGCCAGAC CAGGAGTGAC 
TTCCTGCTGG ATCCAAGCAG GAGTTATAGT TACCCTAGAC AAAAGACGCC AGGCACACCA 
AAGAGAAACT GCCCAGCACC TTTTGATTTT GATGGCTGTG AGCTCCTGGC CAGCCCCACT 
AGCCCAGTCA CTGCAGAATT CAGTAGCAGC GTCTCTGGTT GTCCCAAGTC AGCCAGCTAC 
TCTCTGGAGA GCACAGATGT GAAATCTCTT GCAGCTGGTG TGACAAAGCA GAGTACGTCA 
TGCCCTGCCT TACCCCCCAG GGCTCCAAAA CTAGTGGAAG AGAAGGTCGC CTCCGAAACA 
TCTCCTTTGC CTCTGAAAAT TGATGGTGCT GAGGAAGACC CCAAGTCTGG GTCACCAGAT 
CTCTCGGAGG ACCAGTATTT TGTTAAAAAG GGCATGCAGG ACATCTTCTC TGCCTCCTAC 
CCTTTCTCAT CTCCGCTCCA TCTCCAGCTG GCCCCCAGAT CCTGTGGCGA CGGTTCCCCA 
TGGCAGCCAC CTGCTGACCT ATCAGGACTC TCTATAGAGG AAGTGTCCAA GTCACTACGG 
TTCATTGGTT TGTCCGAAGA TGTCATATCA TTCTTTGTTA CTGAAAAGAT TGATGGGAAC 
CTGCTTGTTC AGCTAACGGA AGAAATCCTC TCAGAGGATT TCAAATTGAG CAAATTGCAG 
GTGAAGAAGA TAATGCAATT CATTAATGGC TGGAGGCCCA AAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073588.1
CDS	57..2684
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDNGECVEGLRENDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGESWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEECKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAVKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNIVTKTDTNPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRLSWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTSPVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASETSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGDGSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKIMQFINGWRPKI

Target ORF information:

RefSeq Version	NM_022751.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022751.2

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCGTCACT 
AAAACTGACA CAAATCCTTC TGAAAGCACT CCTGTTTCCT GCTATCCATG TAACCGAGTG 
AAAACTGATT CTGTGGACCT GAAATCCCCG TTTGGAAGTC CTTCTGCTGA AGCTGTGTCC 
TCTCGGCTCT CATGGCCTAA CCATTATTCA GGAGCATCAG AAAGCCAGAC CAGGAGTGAC 
TTCCTGCTGG ATCCAAGCAG GAGTTATAGT TACCCTAGAC AAAAGACGCC AGGCACACCA 
AAGAGAAACT GCCCAGCACC TTTTGATTTT GATGGCTGTG AGCTCCTGGC CAGCCCCACT 
AGCCCAGTCA CTGCAGAATT CAGTAGCAGC GTCTCTGGTT GTCCCAAGTC AGCCAGCTAC 
TCTCTGGAGA GCACAGATGT GAAATCTCTT GCAGCTGGTG TGACAAAGCA GAGTACGTCA 
TGCCCTGCCT TACCCCCCAG GGCTCCAAAA CTAGTGGAAG AGAAGGTCGC CTCCGAAACA 
TCTCCTTTGC CTCTGAAAAT TGATGGTGCT GAGGAAGACC CCAAGTCTGG GTCACCAGAT 
CTCTCGGAGG ACCAGTATTT TGTTAAAAAG GGCATGCAGG ACATCTTCTC TGCCTCCTAC 
CCTTTCTCAT CTCCGCTCCA TCTCCAGCTG GCCCCCAGAT CCTGTGGCGA CGGTTCCCCA 
TGGCAGCCAC CTGCTGACCT ATCAGGACTC TCTATAGAGG AAGTGTCCAA GTCACTACGG 
TTCATTGGTT TGTCCGAAGA TGTCATATCA TTCTTTGTTA CTGAAAAGAT TGATGGGAAC 
CTGCTTGTTC AGCTAACGGA AGAAATCCTC TCAGAGGATT TCAAATTGAG CAAATTGCAG 
GTGAAGAAGA TAATGCAATT CATTAATGGC TGGAGGCCCA AAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( NM_022751.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_073588.1 Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu00218
Clone ID Related Accession (Same CDS sequence)	NM_022751.2 , NM_022751.3
Accession Version	NM_022751.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	GRB2-associated and regulator of MAPK protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009835.14, AK025263.1, AK025660.1, AC015563.11 and AI479499.1. On May 31, 2019 this sequence version replaced NM_022751.2. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at the 3' terminal exon compared to variant 1. This results in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.91166.1, AK025263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCGTCACT 
AAAACTGACA CAAATCCTTC TGAAAGCACT CCTGTTTCCT GCTATCCATG TAACCGAGTG 
AAAACTGATT CTGTGGACCT GAAATCCCCG TTTGGAAGTC CTTCTGCTGA AGCTGTGTCC 
TCTCGGCTCT CATGGCCTAA CCATTATTCA GGAGCATCAG AAAGCCAGAC CAGGAGTGAC 
TTCCTGCTGG ATCCAAGCAG GAGTTATAGT TACCCTAGAC AAAAGACGCC AGGCACACCA 
AAGAGAAACT GCCCAGCACC TTTTGATTTT GATGGCTGTG AGCTCCTGGC CAGCCCCACT 
AGCCCAGTCA CTGCAGAATT CAGTAGCAGC GTCTCTGGTT GTCCCAAGTC AGCCAGCTAC 
TCTCTGGAGA GCACAGATGT GAAATCTCTT GCAGCTGGTG TGACAAAGCA GAGTACGTCA 
TGCCCTGCCT TACCCCCCAG GGCTCCAAAA CTAGTGGAAG AGAAGGTCGC CTCCGAAACA 
TCTCCTTTGC CTCTGAAAAT TGATGGTGCT GAGGAAGACC CCAAGTCTGG GTCACCAGAT 
CTCTCGGAGG ACCAGTATTT TGTTAAAAAG GGCATGCAGG ACATCTTCTC TGCCTCCTAC 
CCTTTCTCAT CTCCGCTCCA TCTCCAGCTG GCCCCCAGAT CCTGTGGCGA CGGTTCCCCA 
TGGCAGCCAC CTGCTGACCT ATCAGGACTC TCTATAGAGG AAGTGTCCAA GTCACTACGG 
TTCATTGGTT TGTCCGAAGA TGTCATATCA TTCTTTGTTA CTGAAAAGAT TGATGGGAAC 
CTGCTTGTTC AGCTAACGGA AGAAATCCTC TCAGAGGATT TCAAATTGAG CAAATTGCAG 
GTGAAGAAGA TAATGCAATT CATTAATGGC TGGAGGCCCA AAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073588.1
CDS	455..3082
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDNGECVEGLRENDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGESWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEECKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAVKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNIVTKTDTNPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRLSWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTSPVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASETSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGDGSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKIMQFINGWRPKI

Target ORF information:

RefSeq Version	NM_022751.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022751.3

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCGTCACT 
AAAACTGACA CAAATCCTTC TGAAAGCACT CCTGTTTCCT GCTATCCATG TAACCGAGTG 
AAAACTGATT CTGTGGACCT GAAATCCCCG TTTGGAAGTC CTTCTGCTGA AGCTGTGTCC 
TCTCGGCTCT CATGGCCTAA CCATTATTCA GGAGCATCAG AAAGCCAGAC CAGGAGTGAC 
TTCCTGCTGG ATCCAAGCAG GAGTTATAGT TACCCTAGAC AAAAGACGCC AGGCACACCA 
AAGAGAAACT GCCCAGCACC TTTTGATTTT GATGGCTGTG AGCTCCTGGC CAGCCCCACT 
AGCCCAGTCA CTGCAGAATT CAGTAGCAGC GTCTCTGGTT GTCCCAAGTC AGCCAGCTAC 
TCTCTGGAGA GCACAGATGT GAAATCTCTT GCAGCTGGTG TGACAAAGCA GAGTACGTCA 
TGCCCTGCCT TACCCCCCAG GGCTCCAAAA CTAGTGGAAG AGAAGGTCGC CTCCGAAACA 
TCTCCTTTGC CTCTGAAAAT TGATGGTGCT GAGGAAGACC CCAAGTCTGG GTCACCAGAT 
CTCTCGGAGG ACCAGTATTT TGTTAAAAAG GGCATGCAGG ACATCTTCTC TGCCTCCTAC 
CCTTTCTCAT CTCCGCTCCA TCTCCAGCTG GCCCCCAGAT CCTGTGGCGA CGGTTCCCCA 
TGGCAGCCAC CTGCTGACCT ATCAGGACTC TCTATAGAGG AAGTGTCCAA GTCACTACGG 
TTCATTGGTT TGTCCGAAGA TGTCATATCA TTCTTTGTTA CTGAAAAGAT TGATGGGAAC 
CTGCTTGTTC AGCTAACGGA AGAAATCCTC TCAGAGGATT TCAAATTGAG CAAATTGCAG 
GTGAAGAAGA TAATGCAATT CATTAATGGC TGGAGGCCCA AAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_017025919.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881408.1 Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu71459
Clone ID Related Accession (Same CDS sequence)	XM_017025919.1
Accession Version	XM_017025919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GRB2-associated and regulator of MAPK protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATAATGGAA 
GAGCCAATGT CTTGGGAAGA AAAGATGCCA CACCAGTCTG AGAAATTCAG AGGTAAAGGC 
CAACTAGATT CTCCTGGAAA CAACCCGGGG GCTGCCATCA GTTCTCACAA GTCAGCATCT 
GGTTCTTTCC ACTTTGACAC GAGCAGACAG CACCTTCAAG CAGACAACAC CTTCGACCAG 
GACCTAAAAT GGAAAGGGTC CCATTGGCTA TCTACTCTGA ATTTCCATCC AGACAGGAGT 
CCCCTTTTGA CCATCACTGT TGAGTGGTCA TTTCCTTTTG TCTGGATACT TCCAGTGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881408.1
CDS	91..2133
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDNGECVEGLRENDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGESWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEECKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAVKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNIMEEPMSWEEKMPHQSEKFRGKGQLDSPGNNPGAAISSHKSASGSFHFDTSRQHLQADNTFDQDLKWKGSHWLSTLNFHPDRSPLLTITVEWSFPFVWILPVL

Target ORF information:

RefSeq Version	XM_017025919.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025919.1

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG CCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTATCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTCATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGAG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACCGACTGG AATGAAGAAT GCAAGAGCCC CAAGAAGGGT 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTCTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAGGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGCG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAG TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATAATGGAA 
GAGCCAATGT CTTGGGAAGA AAAGATGCCA CACCAGTCTG AGAAATTCAG AGGTAAAGGC 
CAACTAGATT CTCCTGGAAA CAACCCGGGG GCTGCCATCA GTTCTCACAA GTCAGCATCT 
GGTTCTTTCC ACTTTGACAC GAGCAGACAG CACCTTCAAG CAGACAACAC CTTCGACCAG 
GACCTAAAAT GGAAAGGGTC CCATTGGCTA TCTACTCTGA ATTTCCATCC AGACAGGAGT 
CCCCTTTTGA CCATCACTGT TGAGTGGTCA TTTCCTTTTG TCTGGATACT TCCAGTGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_024451234.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307002.1 Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu104569
Clone ID Related Accession (Same CDS sequence)	XM_024451234.1 , XM_024451235.1
Accession Version	XM_024451234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GRB2-associated and regulator of MAPK protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC 
CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG 
AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT 
CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA 
AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA 
ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC 
CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG 
GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT 
GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG 
GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT 
GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC 
TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC 
TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC 
CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC 
TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA 
GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA 
GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA 
GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC 
GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT 
TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC 
TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA 
ACTGACACAA ATCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA 
ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT 
CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC 
CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG 
AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCCTGGCCAG CCCCACTAGC 
CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT 
CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC 
CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT 
CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC 
TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT 
TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGCGACGG TTCCCCATGG 
CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC 
ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG 
CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ATTGCAGGTG 
AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307002.1
CDS	387..2651
Translation	MEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGESWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEECKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAVKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNISVTKTDTNPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRLSWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTSPVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASETSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGDGSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKIMQFINGWRPKI

Target ORF information:

RefSeq Version	XM_024451234.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451234.1

ORF Insert Sequence:

ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC 
CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG 
AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT 
CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA 
AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA 
ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC 
CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG 
GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT 
GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG 
GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT 
GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC 
TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC 
TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC 
CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC 
TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA 
GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA 
GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA 
GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC 
GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT 
TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC 
TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA 
ACTGACACAA ATCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA 
ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT 
CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC 
CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG 
AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCCTGGCCAG CCCCACTAGC 
CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT 
CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC 
CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT 
CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC 
TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT 
TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGCGACGG TTCCCCATGG 
CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC 
ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG 
CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ATTGCAGGTG 
AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_024451235.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307003.1 Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu104569
Clone ID Related Accession (Same CDS sequence)	XM_024451234.1 , XM_024451235.1
Accession Version	XM_024451235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GRB2-associated and regulator of MAPK protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC 
CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG 
AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT 
CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA 
AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA 
ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC 
CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG 
GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT 
GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG 
GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT 
GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC 
TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC 
TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC 
CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC 
TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA 
GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA 
GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA 
GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC 
GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT 
TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC 
TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA 
ACTGACACAA ATCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA 
ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT 
CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC 
CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG 
AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCCTGGCCAG CCCCACTAGC 
CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT 
CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC 
CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT 
CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC 
TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT 
TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGCGACGG TTCCCCATGG 
CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC 
ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG 
CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ATTGCAGGTG 
AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307003.1
CDS	503..2767
Translation	MEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGESWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEECKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAVKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNISVTKTDTNPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRLSWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTSPVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASETSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGDGSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKIMQFINGWRPKI

Target ORF information:

RefSeq Version	XM_024451235.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451235.1

ORF Insert Sequence:

ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC 
CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG 
AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT 
CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA 
AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA 
ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC 
CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG 
GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT 
GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG 
GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT 
GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC 
TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC 
TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC 
CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC 
TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA 
GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA 
GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA 
GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC 
GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT 
TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC 
TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA 
ACTGACACAA ATCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA 
ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT 
CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC 
CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG 
AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCCTGGCCAG CCCCACTAGC 
CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT 
CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC 
CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT 
CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC 
TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT 
TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGCGACGG TTCCCCATGG 
CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC 
ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG 
CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ATTGCAGGTG 
AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_024451236.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307004.1 Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu104570
Clone ID Related Accession (Same CDS sequence)	XM_024451236.1
Accession Version	XM_024451236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GRB2-associated and regulator of MAPK protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC 
CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG 
AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT 
CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA 
AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA 
ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC 
CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG 
GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT 
GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG 
GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT 
GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC 
TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC 
TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC 
CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC 
TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA 
GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA 
GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA 
GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC 
GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT 
TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC 
TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCGT CACTAAAACT 
GACACAAATC CTTCTGAAAG CACTCCTGTT TCCTGCTATC CATGTAACCG AGTGAAAACT 
GATTCTGTGG ACCTGAAATC CCCGTTTGGA AGTCCTTCTG CTGAAGCTGT GTCCTCTCGG 
CTCTCATGGC CTAACCATTA TTCAGGAGCA TCAGAAAGCC AGACCAGGAG TGACTTCCTG 
CTGGATCCAA GCAGGAGTTA TAGTTACCCT AGACAAAAGA CGCCAGGCAC ACCAAAGAGA 
AACTGCCCAG CACCTTTTGA TTTTGATGGC TGTGAGCTCC TGGCCAGCCC CACTAGCCCA 
GTCACTGCAG AATTCAGTAG CAGCGTCTCT GGTTGTCCCA AGTCAGCCAG CTACTCTCTG 
GAGAGCACAG ATGTGAAATC TCTTGCAGCT GGTGTGACAA AGCAGAGTAC GTCATGCCCT 
GCCTTACCCC CCAGGGCTCC AAAACTAGTG GAAGAGAAGG TCGCCTCCGA AACATCTCCT 
TTGCCTCTGA AAATTGATGG TGCTGAGGAA GACCCCAAGT CTGGGTCACC AGATCTCTCG 
GAGGACCAGT ATTTTGTTAA AAAGGGCATG CAGGACATCT TCTCTGCCTC CTACCCTTTC 
TCATCTCCGC TCCATCTCCA GCTGGCCCCC AGATCCTGTG GCGACGGTTC CCCATGGCAG 
CCACCTGCTG ACCTATCAGG ACTCTCTATA GAGGAAGTGT CCAAGTCACT ACGGTTCATT 
GGTTTGTCCG AAGATGTCAT ATCATTCTTT GTTACTGAAA AGATTGATGG GAACCTGCTT 
GTTCAGCTAA CGGAAGAAAT CCTCTCAGAG GATTTCAAAT TGAGCAAATT GCAGGTGAAG 
AAGATAATGC AATTCATTAA TGGCTGGAGG CCCAAAATAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307004.1
CDS	569..2830
Translation	MEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGESWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEECKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAVKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNIVTKTDTNPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRLSWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTSPVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASETSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGDGSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKIMQFINGWRPKI

Target ORF information:

RefSeq Version	XM_024451236.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451236.1

ORF Insert Sequence:

ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC 
CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG 
AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTCAT TTGTATGAAT 
CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA 
AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA 
ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC 
CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG 
GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT 
GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGAGCTGGCC CGAAACCCTG 
GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT 
GTCCGTGATG TGAAAACCGA CTGGAATGAA GAATGCAAGA GCCCCAAGAA GGGTCGGTGC 
TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC 
TTCCACCGAC TCTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC 
CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC 
TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA 
GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA GGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGCGAGAAGA ACAGATGTGA TCAGTTTAGA 
GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA 
GCAGTGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC 
GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT 
TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC 
TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCGT CACTAAAACT 
GACACAAATC CTTCTGAAAG CACTCCTGTT TCCTGCTATC CATGTAACCG AGTGAAAACT 
GATTCTGTGG ACCTGAAATC CCCGTTTGGA AGTCCTTCTG CTGAAGCTGT GTCCTCTCGG 
CTCTCATGGC CTAACCATTA TTCAGGAGCA TCAGAAAGCC AGACCAGGAG TGACTTCCTG 
CTGGATCCAA GCAGGAGTTA TAGTTACCCT AGACAAAAGA CGCCAGGCAC ACCAAAGAGA 
AACTGCCCAG CACCTTTTGA TTTTGATGGC TGTGAGCTCC TGGCCAGCCC CACTAGCCCA 
GTCACTGCAG AATTCAGTAG CAGCGTCTCT GGTTGTCCCA AGTCAGCCAG CTACTCTCTG 
GAGAGCACAG ATGTGAAATC TCTTGCAGCT GGTGTGACAA AGCAGAGTAC GTCATGCCCT 
GCCTTACCCC CCAGGGCTCC AAAACTAGTG GAAGAGAAGG TCGCCTCCGA AACATCTCCT 
TTGCCTCTGA AAATTGATGG TGCTGAGGAA GACCCCAAGT CTGGGTCACC AGATCTCTCG 
GAGGACCAGT ATTTTGTTAA AAAGGGCATG CAGGACATCT TCTCTGCCTC CTACCCTTTC 
TCATCTCCGC TCCATCTCCA GCTGGCCCCC AGATCCTGTG GCGACGGTTC CCCATGGCAG 
CCACCTGCTG ACCTATCAGG ACTCTCTATA GAGGAAGTGT CCAAGTCACT ACGGTTCATT 
GGTTTGTCCG AAGATGTCAT ATCATTCTTT GTTACTGAAA AGATTGATGG GAACCTGCTT 
GTTCAGCTAA CGGAAGAAAT CCTCTCAGAG GATTTCAAAT TGAGCAAATT GCAGGTGAAG 
AAGATAATGC AATTCATTAA TGGCTGGAGG CCCAAAATAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GAREM1 regulates the PR interval on electrocardiograms.
Journal of human genetics(2017 Dec)
Kim HO,Lim JE,Kim MJ,Kang JO,Kim SM,Nam JM,Tak J,Konishi H,Nishino T,Koh IS,Jin YH,Baik HH,Kim JB,Kim MK,Choi BY,Lee SH,Jang Y,Shin J,Oh B

Functional relationship between CABIT, SAM and 14-3-3 binding domains of GAREM1 that play a role in its subcellular localization.
Biochemical and biophysical research communications464(2)616-21(2015 Aug)
Nishino T,Matsunaga R,Konishi H

Phospho-tyrosine dependent protein-protein interaction network.
Molecular systems biology11(3)794(2015 Mar)
Grossmann A,Benlasfer N,Birth P,Hegele A,Wachsmuth F,Apelt L,Stelzl U

Identification of three novel genetic variations associated with electrocardiographic traits (QRS duration and PR interval) in East Asians.
Human molecular genetics23(24)6659-67(2014 Dec)
Hong KW,Lim JE,Kim JW,Tabara Y,Ueshima H,Miki T,Matsuda F,Cho YS,Kim Y,Oh B

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GAREM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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