SI cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI
Entrez Gene ID	6476
Full Name	sucrase-isomaltase
General protein information	Preferred Names sucrase-isomaltase Names sucrase-isomaltase, intestinal alpha-glucosidase oligosaccharide alpha-1,6-glucosidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q26.1
Summary	This gene encodes a sucrase-isomaltase enzyme that is expressed in the intestinal brush border. The encoded protein is synthesized as a precursor protein that is cleaved by pancreatic proteases into two enzymatic subunits sucrase and isomaltase. These two subunits heterodimerize to form the sucrose-isomaltase complex. This complex is essential for the digestion of dietary carbohydrates including starch, sucrose and isomaltose. Mutations in this gene are the cause of congenital sucrase-isomaltase deficiency.[provided by RefSeq, Apr 2010].
Disorder MIM:	609845
Disorder Html:	Sucrase-isomaltase deficiency, congenital, 222900 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001041.4	NP_001032.2	sucrase-isomaltase, intestinal
XM_011513078.2	XP_011511380.1	sucrase-isomaltase, intestinal isoform X1
NM_001041.3	NP_001032.2	sucrase-isomaltase, intestinal

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SI	XP_545265.3
Mus musculus (house mouse)	Sis	NP_001074606.1
Homo sapiens (human)	SI	NP_001032.2
	SI	XP_002802979.1
Bos taurus (cattle)	SI	NP_001107661.1
Pan troglodytes (chimpanzee)	SI	XP_001159286.2
Rattus norvegicus (Norway rat)	Si	NP_037193.1

Gene Ontology
Bibliography

Related articles in PubMed

Congenital Sucrase-isomaltase Deficiency: A Novel Compound Heterozygous Mutation Causing Aberrant Protein Localization.
Haberman Y, Di Segni A, Loberman-Nachum N, Barel O, Kunik V, Eyal E, Kol N, Hout-Siloni G, Kochavi B, Avivi C, Schvimer M, Rechavi G, Anikster Y, Barshack I, Weiss B
Journal of pediatric gastroenterology and nutrition64(5)770-776(2017 May)

Molecular pathogenicity of novel sucrase-isomaltase mutations found in congenital sucrase-isomaltase deficiency patients.
Gericke B, Amiri M, Scott CR, Naim HY
Biochimica et biophysica acta1863(3)817-826(2017 Mar)

Congenital sucrase-isomaltase deficiency: identification of a common Inuit founder mutation.
Marcadier JL, Boland M, Scott CR, Issa K, Wu Z, McIntyre AD, Hegele RA, Geraghty MT, Lines MA
CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne187(2)102-7(2015 Feb)

Functional analysis of sucrase-isomaltase mutations from chronic lymphocytic leukemia patients.
Rodr?guez D, Ramsay AJ, Quesada V, Garabaya C, Campo E, Freije JM, L?pez-Ot?n C
Human molecular genetics22(11)2273-82(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A common mutation was found in the sucrase-isomaltase gene, c.273_274delAG, to be responsible for the high prevalence of congenital sucrase-isomaltase deficiency among Inuit people.
Title: Congenital sucrase-isomaltase deficiency: identification of a common Inuit founder mutation.

SI mutations result in loss of enzyme function by preventing the biosynthesis of catalytically competent SI at the cell surface in lymphocytic leukemia
Title: Functional analysis of sucrase-isomaltase mutations from chronic lymphocytic leukemia patients.

study found four common mutations in the SI gene (3 of the 4 are in the sucrase domain, with 1 in the isomaltase domain) account for 59 percent of clinical symptoms of congenital sucrase-isomaltase deficiency (CSID); the remaining 41 percent were rare events
Title: Four mutations in the SI gene are responsible for the majority of clinical symptoms of CSID.

investigation of EIS (enzyme-inhibitor-substrate) complex of sucrase: kinetic studies of complex formation/stability; role of complex in prevention of hyperglycemia by L-arabinose
Title: Determination of the transient period of the EIS complex and investigation of the suppression of blood glucose levels by L-arabinose in healthy adults.

Core2 O-glycan structure is essential for expression of SI and DDP-IV during intestinal cell differentiation.
Title: Core2 O-glycan structure is essential for the cell surface expression of sucrase isomaltase and dipeptidyl peptidase-IV during intestinal cell differentiation.

The following SI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20987	NM_001041.4 Latest version!	Homo sapiens sucrase-isomaltase (SI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu60497	XM_011513078.2 Latest version!	Homo sapiens sucrase-isomaltase (SI), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu20987	NM_001041.3	Homo sapiens sucrase-isomaltase (SI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI ( NM_001041.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001032.2 Homo sapiens sucrase-isomaltase (SI), mRNA.

Price & Availability↑

CloneID	OHu20987
Clone ID Related Accession (Same CDS sequence)	NM_001041.4 , NM_001041.3
Accession Version	NM_001041.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	sucrase-isomaltase, intestinal
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC140119.3, DA919374.1, BC132860.1, AC092695.8 and BG187733.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001041.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X63597.1, BC132834.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264382.8/ ENSP00000264382.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAAGAA AGAAATTTAG TGGATTGGAA ATCTCTCTGA TTGTCCTTTT TGTCATAGTT 
ACTATAATAG CTATTGCCTT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGATGAA 
ATTAGTGATT CTACTTCAAC TCCAGCTACT ACTCGTGTGA CTACAAATCC TTCTGATTCA 
GGAAAATGTC CAAATGTGTT AAATGATCCT GTCAATGTGA GAATAAACTG CATTCCAGAA 
CAATTCCCAA CAGAGGGAAT TTGTGCACAG AGAGGCTGCT GCTGGAGGCC GTGGAATGAC 
TCTCTTATTC CTTGGTGCTT CTTCGTTGAT AATCATGGTT ATAACGTTCA AGACATGACA 
ACAACAAGTA TTGGAGTTGA AGCCAAATTA AACAGGATAC CTTCACCTAC ACTATTTGGA 
AATGACATCA ACAGTGTTCT CTTCACAACT CAAAATCAGA CACCCAATCG TTTCCGGTTC 
AAGATTACTG ATCCAAATAA TAGAAGATAT GAAGTTCCTC ATCAGTATGT AAAAGAGTTT 
ACTGGACCCA CAGTTTCTGA TACGTTGTAT GATGTGAAGG TTGCCCAAAA CCCATTTAGC 
ATCCAAGTTA TTAGGAAAAG CAACGGTAAA ACTTTGTTTG ACACCAGCAT TGGTCCCTTA 
GTGTACTCTG ACCAGTACTT ACAGATCTCA ACCCGTCTTC CAAGTGATTA TATTTATGGT 
ATTGGAGAAC AAGTTCATAA GAGATTTCGT CATGATTTAT CCTGGAAAAC ATGGCCAATT 
TTTACTCGAG ACCAACTTCC TGGTGATAAT AATAATAATT TATACGGCCA TCAAACATTC 
TTTATGTGTA TTGAAGATAC ATCTGGAAAG TCATTCGGTG TTTTTTTAAT GAATAGCAAT 
GCAATGGAGA TTTTTATCCA GCCTACTCCA ATAGTAACAT ATAGAGTTAC CGGTGGCATT 
CTGGATTTTT ACATCCTTCT AGGAGATACA CCAGAACAAG TAGTTCAACA GTATCAACAG 
CTTGTTGGAC TACCAGCAAT GCCAGCATAT TGGAATCTTG GATTCCAACT AAGTCGCTGG 
AATTATAAGT CACTAGATGT AGTGAAAGAA GTGGTAAGGA GAAACCGGGA AGCTGGCATA 
CCATTTGATA CACAGGTCAC TGATATTGAC TACATGGAAG ACAAGAAAGA CTTTACTTAT 
GATCAAGTTG CGTTTAACGG ACTCCCTCAA TTTGTGCAAG ATTTGCATGA CCATGGACAG 
AAATATGTCA TCATCTTGGA CCCTGCAATT TCCATAGGTC GACGTGCCAA TGGAACAACA 
TATGCAACCT ATGAGAGGGG AAACACACAA CATGTGTGGA TAAATGAGTC AGATGGAAGT 
ACACCAATTA TTGGAGAGGT ATGGCCAGGA TTAACAGTAT ACCCTGATTT CACTAACCCA 
AACTGCATTG ATTGGTGGGC AAATGAATGC AGTATTTTCC ATCAAGAAGT GCAATATGAT 
GGACTTTGGA TTGACATGAA TGAAGTTTCC AGCTTTATTC AAGGTTCAAC AAAAGGATGT 
AATGTAAACA AATTGAATTA TCCACCGTTT ACTCCTGATA TTCTTGACAA ACTCATGTAT 
TCCAAAACAA TTTGCATGGA TGCTGTGCAG AACTGGGGTA AACAGTATGA TGTTCATAGC 
CTCTATGGAT ACAGCATGGC TATAGCCACA GAGCAAGCTG TACAAAAAGT TTTTCCTAAT 
AAGAGAAGCT TCATTCTTAC CCGCTCAACA TTTGCTGGAT CTGGAAGACA TGCTGCGCAT 
TGGTTAGGAG ACAATACTGC TTCATGGGAA CAAATGGAAT GGTCTATAAC TGGAATGCTG 
GAGTTCAGTT TGTTTGGAAT ACCTTTGGTT GGAGCAGACA TCTGTGGATT TGTGGCTGAA 
ACCACAGAAG AACTTTGCAG AAGATGGATG CAACTTGGGG CATTTTATCC ATTTTCCAGA 
AACCATAATT CTGACGGATA TGAACATCAG GATCCTGCAT TTTTTGGGCA GAATTCACTT 
TTGGTTAAAT CATCAAGGCA GTATTTAACT ATTCGCTACA CCTTATTACC CTTCCTCTAC 
ACTCTGTTTT ATAAAGCCCA TGTGTTTGGA GAAACAGTAG CAAGACCAGT TCTTCATGAG 
TTTTATGAGG ATACGAACAG CTGGATTGAG GACACTGAGT TTTTGTGGGG CCCTGCATTA 
CTTATTACTC CTGTTCTAAA ACAGGGAGCA GATACTGTGA GTGCCTACAT CCCTGATGCT 
ATTTGGTATG ATTATGAATC TGGTGCAAAA AGGCCATGGA GGAAACAACG GGTTGATATG 
TATCTTCCAG CAGACAAAAT AGGATTACAT CTTAGAGGAG GTTATATCAT CCCCATTCAA 
GAACCAGATG TAACAACAAC AGCAAGCCGT AAGAATCCTC TAGGACTTAT AGTCGCATTA 
GGTGAAAACA ACACAGCCAA AGGAGACTTT TTCTGGGATG ATGGAGAAAC TAAAGATACA 
ATACAAAATG GCAACTACAT ATTATATACA TTTTCAGTTT CTAATAACAC ATTAGATATT 
GTGTGCACAC ATTCATCATA TCAGGAAGGA ACTACCTTAG CATTTCAGAC TGTAAAAATC 
CTTGGGTTGA CAGACAGTGT TACAGAAGTT AGAGTGGCGG AAAATAATCA ACCAATGAAC 
GCTCATTCCA ATTTCACTTA TGATGCTTCT AACCAGGTTC TCCTAATTGC AGATCTCAAA 
CTTAATCTTG GAAGAAACTT TAGTGTTCAA TGGAATCAAA TTTTCTCAGA AAATGAAAGA 
TTTAATTGTT ATCCAGATGC AGATTTGGCA ACTGAACAAA AGTGCACACA ACGTGGCTGT 
GTATGGAGAA CGGGTTCTTC TCTATCCAAA GCACCTGAGT GTTACTTTCC CAGACAAGAT 
AACTCTTATT CAGTCAACTC AGCTCGCTAT TCATCCATGG GTATAACAGC TGACCTCCAA 
CTAAATACTG CAAATGCCAG AATAAAGTTA CCTTCTGACC CCATCTCAAC TCTTCGTGTG 
GAGGTGAAAT ATCACAAAAA TGATATGTTG CAGTTTAAGA TTTATGATCC CCAAAAGAAG 
AGATATGAAG TACCAGTACC GTTAAACATT CCAACCACCC CAATAAGTAC TTATGAAGAC 
AGACTTTATG ATGTGGAAAT CAAGGAAAAT CCTTTTGGCA TCCAGATTCG ACGGAGAAGC 
AGTGGAAGAG TCATTTGGGA TTCTTGGCTG CCTGGATTTG CTTTTAATGA CCAGTTCATT 
CAAATATCGA CTCGCCTGCC ATCAGAATAT ATATATGGTT TTGGGGAAGT GGAACATACA 
GCATTTAAGC GAGATCTGAA CTGGAATACT TGGGGAATGT TCACAAGAGA CCAACCCCCT 
GGTTACAAAC TTAATTCCTA TGGATTTCAT CCCTATTACA TGGCTCTGGA AGAGGAGGGC 
AATGCTCATG GTGTTTTCTT ACTCAACAGC AATGCAATGG ATGTTACATT CCAGCCAACT 
CCTGCTCTAA CTTACCGTAC AGTTGGAGGG ATCTTGGATT TTTATATGTT TTTGGGCCCA 
ACTCCAGAAG TTGCAACAAA GCAATACCAT GAAGTAATTG GCCATCCAGT CATGCCAGCT 
TATTGGGCTT TGGGATTCCA ATTATGTCGT TATGGATATG CAAATACTTC AGAGGTTCGG 
GAATTATATG ACGCTATGGT GGCTGCTAAC ATCCCCTATG ATGTTCAGTA CACAGACATT 
GACTACATGG AAAGGCAGCT AGACTTTACA ATTGGTGAAG CATTCCAGGA CCTTCCTCAG 
TTTGTTGACA AAATAAGAGG AGAAGGAATG AGATACATTA TTATCCTGGA TCCAGCAATT 
TCAGGAAATG AAACAAAGAC TTACCCTGCA TTTGAAAGAG GACAGCAGAA TGATGTCTTT 
GTCAAATGGC CAAACACCAA TGACATTTGT TGGGCAAAGG TTTGGCCAGA TTTGCCCAAC 
ATAACAATAG ATAAAACTCT AACGGAAGAT GAAGCTGTTA ATGCTTCCAG AGCTCATGTA 
GCTTTCCCAG ATTTCTTCAG GACTTCCACA GCAGAGTGGT GGGCCAGAGA AATTGTGGAC 
TTTTACAATG AAAAGATGAA GTTTGATGGT TTGTGGATTG ATATGAATGA GCCATCAAGT 
TTTGTAAATG GAACAACTAC TAATCAATGC AGAAATGACG AACTAAATTA TCCACCTTAT 
TTCCCAGAAC TCACAAAAAG AACTGATGGA TTACATTTCA GAACAATTTG CATGGAAGCT 
GAGCAGATTC TTAGTGATGG AACATCAGTT TTGCATTACG ATGTTCACAA TCTCTATGGA 
TGGTCACAGA TGAAACCTAC TCATGATGCA TTGCAGAAGA CAACTGGAAA AAGAGGGATT 
GTAATTTCTC GTTCCACGTA TCCTACTAGT GGACGATGGG GAGGACACTG GCTTGGAGAC 
AACTATGCAC GATGGGACAA CATGGACAAA TCAATCATTG GTATGATGGA ATTTAGTCTG 
TTTGGAATGT CATATACTGG AGCAGACATC TGTGGTTTTT TCAACAACTC AGAATATCAT 
CTCTGTACCC GCTGGATGCA ACTTGGAGCA TTTTATCCAT ACTCAAGGAA TCACAACATT 
GCAAATACTA GAAGACAAGA TCCCGCTTCC TGGAATGAAA CTTTTGCTGA AATGTCAAGG 
AATATTCTAA ATATTAGATA CACCTTATTG CCCTATTTTT ACACACAAAT GCATGAAATT 
CATGCTAATG GTGGCACTGT TATCCGACCC CTTTTGCATG AGTTCTTTGA TGAAAAACCA 
ACCTGGGATA TATTCAAGCA GTTCTTATGG GGTCCAGCAT TTATGGTTAC CCCAGTACTG 
GAACCTTATG TTCAAACTGT AAATGCCTAC GTCCCCAATG CTCGGTGGTT TGACTACCAT 
ACAGGCAAAG ATATTGGCGT CAGAGGACAA TTTCAAACAT TTAATGCTTC TTATGACACA 
ATAAACCTAC ATGTCCGTGG TGGTCACATC CTACCATGTC AAGAGCCAGC TCAAAACACA 
TTTTACAGTC GACAAAAACA CATGAAGCTC ATTGTTGCTG CAGATGATAA TCAGATGGCA 
CAGGGTTCTC TGTTTTGGGA TGATGGAGAG AGTATAGACA CCTATGAAAG AGACCTATAT 
TTATCTGTAC AATTTAATTT AAACCAGACC ACCTTAACAA GCACTATATT GAAGAGAGGT 
TACATAAATA AAAGTGAAAC GAGGCTTGGA TCCCTTCATG TATGGGGGAA AGGAACTACT 
CCTGTCAATG CAGTTACTCT AACGTATAAC GGAAATAAAA ATTCGCTTCC TTTTAATGAA 
GACACTACCA ACATGATATT ACGTATTGAT CTGACCACAC ACAATGTTAC TCTAGAAGAA 
CCAATAGAAA TCAACTGGTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032.2
CDS	65..5548
Translation	MARKKFSGLEISLIVLFVIVTIIAIALIVVLATKTPAVDEISDSTSTPATTRVTTNPSDSGKCPNVLNDPVNVRINCIPEQFPTEGICAQRGCCWRPWNDSLIPWCFFVDNHGYNVQDMTTTSIGVEAKLNRIPSPTLFGNDINSVLFTTQNQTPNRFRFKITDPNNRRYEVPHQYVKEFTGPTVSDTLYDVKVAQNPFSIQVIRKSNGKTLFDTSIGPLVYSDQYLQISTRLPSDYIYGIGEQVHKRFRHDLSWKTWPIFTRDQLPGDNNNNLYGHQTFFMCIEDTSGKSFGVFLMNSNAMEIFIQPTPIVTYRVTGGILDFYILLGDTPEQVVQQYQQLVGLPAMPAYWNLGFQLSRWNYKSLDVVKEVVRRNREAGIPFDTQVTDIDYMEDKKDFTYDQVAFNGLPQFVQDLHDHGQKYVIILDPAISIGRRANGTTYATYERGNTQHVWINESDGSTPIIGEVWPGLTVYPDFTNPNCIDWWANECSIFHQEVQYDGLWIDMNEVSSFIQGSTKGCNVNKLNYPPFTPDILDKLMYSKTICMDAVQNWGKQYDVHSLYGYSMAIATEQAVQKVFPNKRSFILTRSTFAGSGRHAAHWLGDNTASWEQMEWSITGMLEFSLFGIPLVGADICGFVAETTEELCRRWMQLGAFYPFSRNHNSDGYEHQDPAFFGQNSLLVKSSRQYLTIRYTLLPFLYTLFYKAHVFGETVARPVLHEFYEDTNSWIEDTEFLWGPALLITPVLKQGADTVSAYIPDAIWYDYESGAKRPWRKQRVDMYLPADKIGLHLRGGYIIPIQEPDVTTTASRKNPLGLIVALGENNTAKGDFFWDDGETKDTIQNGNYILYTFSVSNNTLDIVCTHSSYQEGTTLAFQTVKILGLTDSVTEVRVAENNQPMNAHSNFTYDASNQVLLIADLKLNLGRNFSVQWNQIFSENERFNCYPDADLATEQKCTQRGCVWRTGSSLSKAPECYFPRQDNSYSVNSARYSSMGITADLQLNTANARIKLPSDPISTLRVEVKYHKNDMLQFKIYDPQKKRYEVPVPLNIPTTPISTYEDRLYDVEIKENPFGIQIRRRSSGRVIWDSWLPGFAFNDQFIQISTRLPSEYIYGFGEVEHTAFKRDLNWNTWGMFTRDQPPGYKLNSYGFHPYYMALEEEGNAHGVFLLNSNAMDVTFQPTPALTYRTVGGILDFYMFLGPTPEVATKQYHEVIGHPVMPAYWALGFQLCRYGYANTSEVRELYDAMVAANIPYDVQYTDIDYMERQLDFTIGEAFQDLPQFVDKIRGEGMRYIIILDPAISGNETKTYPAFERGQQNDVFVKWPNTNDICWAKVWPDLPNITIDKTLTEDEAVNASRAHVAFPDFFRTSTAEWWAREIVDFYNEKMKFDGLWIDMNEPSSFVNGTTTNQCRNDELNYPPYFPELTKRTDGLHFRTICMEAEQILSDGTSVLHYDVHNLYGWSQMKPTHDALQKTTGKRGIVISRSTYPTSGRWGGHWLGDNYARWDNMDKSIIGMMEFSLFGMSYTGADICGFFNNSEYHLCTRWMQLGAFYPYSRNHNIANTRRQDPASWNETFAEMSRNILNIRYTLLPYFYTQMHEIHANGGTVIRPLLHEFFDEKPTWDIFKQFLWGPAFMVTPVLEPYVQTVNAYVPNARWFDYHTGKDIGVRGQFQTFNASYDTINLHVRGGHILPCQEPAQNTFYSRQKHMKLIVAADDNQMAQGSLFWDDGESIDTYERDLYLSVQFNLNQTTLTSTILKRGYINKSETRLGSLHVWGKGTTPVNAVTLTYNGNKNSLPFNEDTTNMILRIDLTTHNVTLEEPIEINWS

Target ORF information:

RefSeq Version	NM_001041.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sucrase-isomaltase (SI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001041.4

ORF Insert Sequence:

ATGGCAAGAA AGAAATTTAG TGGATTGGAA ATCTCTCTGA TTGTCCTTTT TGTCATAGTT 
ACTATAATAG CTATTGCCTT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGATGAA 
ATTAGTGATT CTACTTCAAC TCCAGCTACT ACTCGTGTGA CTACAAATCC TTCTGATTCA 
GGAAAATGTC CAAATGTGTT AAATGATCCT GTCAATGTGA GAATAAACTG CATTCCAGAA 
CAATTCCCAA CAGAGGGAAT TTGTGCACAG AGAGGCTGCT GCTGGAGGCC GTGGAATGAC 
TCTCTTATTC CTTGGTGCTT CTTCGTTGAT AATCATGGTT ATAACGTTCA AGACATGACA 
ACAACAAGTA TTGGAGTTGA AGCCAAATTA AACAGGATAC CTTCACCTAC ACTATTTGGA 
AATGACATCA ACAGTGTTCT CTTCACAACT CAAAATCAGA CACCCAATCG TTTCCGGTTC 
AAGATTACTG ATCCAAATAA TAGAAGATAT GAAGTTCCTC ATCAGTATGT AAAAGAGTTT 
ACTGGACCCA CAGTTTCTGA TACGTTGTAT GATGTGAAGG TTGCCCAAAA CCCATTTAGC 
ATCCAAGTTA TTAGGAAAAG CAACGGTAAA ACTTTGTTTG ACACCAGCAT TGGTCCCTTA 
GTGTACTCTG ACCAGTACTT ACAGATCTCA ACCCGTCTTC CAAGTGATTA TATTTATGGT 
ATTGGAGAAC AAGTTCATAA GAGATTTCGT CATGATTTAT CCTGGAAAAC ATGGCCAATT 
TTTACTCGAG ACCAACTTCC TGGTGATAAT AATAATAATT TATACGGCCA TCAAACATTC 
TTTATGTGTA TTGAAGATAC ATCTGGAAAG TCATTCGGTG TTTTTTTAAT GAATAGCAAT 
GCAATGGAGA TTTTTATCCA GCCTACTCCA ATAGTAACAT ATAGAGTTAC CGGTGGCATT 
CTGGATTTTT ACATCCTTCT AGGAGATACA CCAGAACAAG TAGTTCAACA GTATCAACAG 
CTTGTTGGAC TACCAGCAAT GCCAGCATAT TGGAATCTTG GATTCCAACT AAGTCGCTGG 
AATTATAAGT CACTAGATGT AGTGAAAGAA GTGGTAAGGA GAAACCGGGA AGCTGGCATA 
CCATTTGATA CACAGGTCAC TGATATTGAC TACATGGAAG ACAAGAAAGA CTTTACTTAT 
GATCAAGTTG CGTTTAACGG ACTCCCTCAA TTTGTGCAAG ATTTGCATGA CCATGGACAG 
AAATATGTCA TCATCTTGGA CCCTGCAATT TCCATAGGTC GACGTGCCAA TGGAACAACA 
TATGCAACCT ATGAGAGGGG AAACACACAA CATGTGTGGA TAAATGAGTC AGATGGAAGT 
ACACCAATTA TTGGAGAGGT ATGGCCAGGA TTAACAGTAT ACCCTGATTT CACTAACCCA 
AACTGCATTG ATTGGTGGGC AAATGAATGC AGTATTTTCC ATCAAGAAGT GCAATATGAT 
GGACTTTGGA TTGACATGAA TGAAGTTTCC AGCTTTATTC AAGGTTCAAC AAAAGGATGT 
AATGTAAACA AATTGAATTA TCCACCGTTT ACTCCTGATA TTCTTGACAA ACTCATGTAT 
TCCAAAACAA TTTGCATGGA TGCTGTGCAG AACTGGGGTA AACAGTATGA TGTTCATAGC 
CTCTATGGAT ACAGCATGGC TATAGCCACA GAGCAAGCTG TACAAAAAGT TTTTCCTAAT 
AAGAGAAGCT TCATTCTTAC CCGCTCAACA TTTGCTGGAT CTGGAAGACA TGCTGCGCAT 
TGGTTAGGAG ACAATACTGC TTCATGGGAA CAAATGGAAT GGTCTATAAC TGGAATGCTG 
GAGTTCAGTT TGTTTGGAAT ACCTTTGGTT GGAGCAGACA TCTGTGGATT TGTGGCTGAA 
ACCACAGAAG AACTTTGCAG AAGATGGATG CAACTTGGGG CATTTTATCC ATTTTCCAGA 
AACCATAATT CTGACGGATA TGAACATCAG GATCCTGCAT TTTTTGGGCA GAATTCACTT 
TTGGTTAAAT CATCAAGGCA GTATTTAACT ATTCGCTACA CCTTATTACC CTTCCTCTAC 
ACTCTGTTTT ATAAAGCCCA TGTGTTTGGA GAAACAGTAG CAAGACCAGT TCTTCATGAG 
TTTTATGAGG ATACGAACAG CTGGATTGAG GACACTGAGT TTTTGTGGGG CCCTGCATTA 
CTTATTACTC CTGTTCTAAA ACAGGGAGCA GATACTGTGA GTGCCTACAT CCCTGATGCT 
ATTTGGTATG ATTATGAATC TGGTGCAAAA AGGCCATGGA GGAAACAACG GGTTGATATG 
TATCTTCCAG CAGACAAAAT AGGATTACAT CTTAGAGGAG GTTATATCAT CCCCATTCAA 
GAACCAGATG TAACAACAAC AGCAAGCCGT AAGAATCCTC TAGGACTTAT AGTCGCATTA 
GGTGAAAACA ACACAGCCAA AGGAGACTTT TTCTGGGATG ATGGAGAAAC TAAAGATACA 
ATACAAAATG GCAACTACAT ATTATATACA TTTTCAGTTT CTAATAACAC ATTAGATATT 
GTGTGCACAC ATTCATCATA TCAGGAAGGA ACTACCTTAG CATTTCAGAC TGTAAAAATC 
CTTGGGTTGA CAGACAGTGT TACAGAAGTT AGAGTGGCGG AAAATAATCA ACCAATGAAC 
GCTCATTCCA ATTTCACTTA TGATGCTTCT AACCAGGTTC TCCTAATTGC AGATCTCAAA 
CTTAATCTTG GAAGAAACTT TAGTGTTCAA TGGAATCAAA TTTTCTCAGA AAATGAAAGA 
TTTAATTGTT ATCCAGATGC AGATTTGGCA ACTGAACAAA AGTGCACACA ACGTGGCTGT 
GTATGGAGAA CGGGTTCTTC TCTATCCAAA GCACCTGAGT GTTACTTTCC CAGACAAGAT 
AACTCTTATT CAGTCAACTC AGCTCGCTAT TCATCCATGG GTATAACAGC TGACCTCCAA 
CTAAATACTG CAAATGCCAG AATAAAGTTA CCTTCTGACC CCATCTCAAC TCTTCGTGTG 
GAGGTGAAAT ATCACAAAAA TGATATGTTG CAGTTTAAGA TTTATGATCC CCAAAAGAAG 
AGATATGAAG TACCAGTACC GTTAAACATT CCAACCACCC CAATAAGTAC TTATGAAGAC 
AGACTTTATG ATGTGGAAAT CAAGGAAAAT CCTTTTGGCA TCCAGATTCG ACGGAGAAGC 
AGTGGAAGAG TCATTTGGGA TTCTTGGCTG CCTGGATTTG CTTTTAATGA CCAGTTCATT 
CAAATATCGA CTCGCCTGCC ATCAGAATAT ATATATGGTT TTGGGGAAGT GGAACATACA 
GCATTTAAGC GAGATCTGAA CTGGAATACT TGGGGAATGT TCACAAGAGA CCAACCCCCT 
GGTTACAAAC TTAATTCCTA TGGATTTCAT CCCTATTACA TGGCTCTGGA AGAGGAGGGC 
AATGCTCATG GTGTTTTCTT ACTCAACAGC AATGCAATGG ATGTTACATT CCAGCCAACT 
CCTGCTCTAA CTTACCGTAC AGTTGGAGGG ATCTTGGATT TTTATATGTT TTTGGGCCCA 
ACTCCAGAAG TTGCAACAAA GCAATACCAT GAAGTAATTG GCCATCCAGT CATGCCAGCT 
TATTGGGCTT TGGGATTCCA ATTATGTCGT TATGGATATG CAAATACTTC AGAGGTTCGG 
GAATTATATG ACGCTATGGT GGCTGCTAAC ATCCCCTATG ATGTTCAGTA CACAGACATT 
GACTACATGG AAAGGCAGCT AGACTTTACA ATTGGTGAAG CATTCCAGGA CCTTCCTCAG 
TTTGTTGACA AAATAAGAGG AGAAGGAATG AGATACATTA TTATCCTGGA TCCAGCAATT 
TCAGGAAATG AAACAAAGAC TTACCCTGCA TTTGAAAGAG GACAGCAGAA TGATGTCTTT 
GTCAAATGGC CAAACACCAA TGACATTTGT TGGGCAAAGG TTTGGCCAGA TTTGCCCAAC 
ATAACAATAG ATAAAACTCT AACGGAAGAT GAAGCTGTTA ATGCTTCCAG AGCTCATGTA 
GCTTTCCCAG ATTTCTTCAG GACTTCCACA GCAGAGTGGT GGGCCAGAGA AATTGTGGAC 
TTTTACAATG AAAAGATGAA GTTTGATGGT TTGTGGATTG ATATGAATGA GCCATCAAGT 
TTTGTAAATG GAACAACTAC TAATCAATGC AGAAATGACG AACTAAATTA TCCACCTTAT 
TTCCCAGAAC TCACAAAAAG AACTGATGGA TTACATTTCA GAACAATTTG CATGGAAGCT 
GAGCAGATTC TTAGTGATGG AACATCAGTT TTGCATTACG ATGTTCACAA TCTCTATGGA 
TGGTCACAGA TGAAACCTAC TCATGATGCA TTGCAGAAGA CAACTGGAAA AAGAGGGATT 
GTAATTTCTC GTTCCACGTA TCCTACTAGT GGACGATGGG GAGGACACTG GCTTGGAGAC 
AACTATGCAC GATGGGACAA CATGGACAAA TCAATCATTG GTATGATGGA ATTTAGTCTG 
TTTGGAATGT CATATACTGG AGCAGACATC TGTGGTTTTT TCAACAACTC AGAATATCAT 
CTCTGTACCC GCTGGATGCA ACTTGGAGCA TTTTATCCAT ACTCAAGGAA TCACAACATT 
GCAAATACTA GAAGACAAGA TCCCGCTTCC TGGAATGAAA CTTTTGCTGA AATGTCAAGG 
AATATTCTAA ATATTAGATA CACCTTATTG CCCTATTTTT ACACACAAAT GCATGAAATT 
CATGCTAATG GTGGCACTGT TATCCGACCC CTTTTGCATG AGTTCTTTGA TGAAAAACCA 
ACCTGGGATA TATTCAAGCA GTTCTTATGG GGTCCAGCAT TTATGGTTAC CCCAGTACTG 
GAACCTTATG TTCAAACTGT AAATGCCTAC GTCCCCAATG CTCGGTGGTT TGACTACCAT 
ACAGGCAAAG ATATTGGCGT CAGAGGACAA TTTCAAACAT TTAATGCTTC TTATGACACA 
ATAAACCTAC ATGTCCGTGG TGGTCACATC CTACCATGTC AAGAGCCAGC TCAAAACACA 
TTTTACAGTC GACAAAAACA CATGAAGCTC ATTGTTGCTG CAGATGATAA TCAGATGGCA 
CAGGGTTCTC TGTTTTGGGA TGATGGAGAG AGTATAGACA CCTATGAAAG AGACCTATAT 
TTATCTGTAC AATTTAATTT AAACCAGACC ACCTTAACAA GCACTATATT GAAGAGAGGT 
TACATAAATA AAAGTGAAAC GAGGCTTGGA TCCCTTCATG TATGGGGGAA AGGAACTACT 
CCTGTCAATG CAGTTACTCT AACGTATAAC GGAAATAAAA ATTCGCTTCC TTTTAATGAA 
GACACTACCA ACATGATATT ACGTATTGAT CTGACCACAC ACAATGTTAC TCTAGAAGAA 
CCAATAGAAA TCAACTGGTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI ( NM_001041.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001032.2 Homo sapiens sucrase-isomaltase (SI), mRNA.

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CloneID	OHu20987
Clone ID Related Accession (Same CDS sequence)	NM_001041.4 , NM_001041.3
Accession Version	NM_001041.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	sucrase-isomaltase, intestinal
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA919374.1, BC132860.1, AC140119.3, AC092695.8 and BG187733.1. This sequence is a reference standard in the RefSeqGene project. On Apr 2, 2010 this sequence version replaced NM_001041.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X63597.1, BC132834.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGAA AGAAATTTAG TGGATTGGAA ATCTCTCTGA TTGTCCTTTT TGTCATAGTT 
ACTATAATAG CTATTGCCTT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGATGAA 
ATTAGTGATT CTACTTCAAC TCCAGCTACT ACTCGTGTGA CTACAAATCC TTCTGATTCA 
GGAAAATGTC CAAATGTGTT AAATGATCCT GTCAATGTGA GAATAAACTG CATTCCAGAA 
CAATTCCCAA CAGAGGGAAT TTGTGCACAG AGAGGCTGCT GCTGGAGGCC GTGGAATGAC 
TCTCTTATTC CTTGGTGCTT CTTCGTTGAT AATCATGGTT ATAACGTTCA AGACATGACA 
ACAACAAGTA TTGGAGTTGA AGCCAAATTA AACAGGATAC CTTCACCTAC ACTATTTGGA 
AATGACATCA ACAGTGTTCT CTTCACAACT CAAAATCAGA CACCCAATCG TTTCCGGTTC 
AAGATTACTG ATCCAAATAA TAGAAGATAT GAAGTTCCTC ATCAGTATGT AAAAGAGTTT 
ACTGGACCCA CAGTTTCTGA TACGTTGTAT GATGTGAAGG TTGCCCAAAA CCCATTTAGC 
ATCCAAGTTA TTAGGAAAAG CAACGGTAAA ACTTTGTTTG ACACCAGCAT TGGTCCCTTA 
GTGTACTCTG ACCAGTACTT ACAGATCTCA ACCCGTCTTC CAAGTGATTA TATTTATGGT 
ATTGGAGAAC AAGTTCATAA GAGATTTCGT CATGATTTAT CCTGGAAAAC ATGGCCAATT 
TTTACTCGAG ACCAACTTCC TGGTGATAAT AATAATAATT TATACGGCCA TCAAACATTC 
TTTATGTGTA TTGAAGATAC ATCTGGAAAG TCATTCGGTG TTTTTTTAAT GAATAGCAAT 
GCAATGGAGA TTTTTATCCA GCCTACTCCA ATAGTAACAT ATAGAGTTAC CGGTGGCATT 
CTGGATTTTT ACATCCTTCT AGGAGATACA CCAGAACAAG TAGTTCAACA GTATCAACAG 
CTTGTTGGAC TACCAGCAAT GCCAGCATAT TGGAATCTTG GATTCCAACT AAGTCGCTGG 
AATTATAAGT CACTAGATGT AGTGAAAGAA GTGGTAAGGA GAAACCGGGA AGCTGGCATA 
CCATTTGATA CACAGGTCAC TGATATTGAC TACATGGAAG ACAAGAAAGA CTTTACTTAT 
GATCAAGTTG CGTTTAACGG ACTCCCTCAA TTTGTGCAAG ATTTGCATGA CCATGGACAG 
AAATATGTCA TCATCTTGGA CCCTGCAATT TCCATAGGTC GACGTGCCAA TGGAACAACA 
TATGCAACCT ATGAGAGGGG AAACACACAA CATGTGTGGA TAAATGAGTC AGATGGAAGT 
ACACCAATTA TTGGAGAGGT ATGGCCAGGA TTAACAGTAT ACCCTGATTT CACTAACCCA 
AACTGCATTG ATTGGTGGGC AAATGAATGC AGTATTTTCC ATCAAGAAGT GCAATATGAT 
GGACTTTGGA TTGACATGAA TGAAGTTTCC AGCTTTATTC AAGGTTCAAC AAAAGGATGT 
AATGTAAACA AATTGAATTA TCCACCGTTT ACTCCTGATA TTCTTGACAA ACTCATGTAT 
TCCAAAACAA TTTGCATGGA TGCTGTGCAG AACTGGGGTA AACAGTATGA TGTTCATAGC 
CTCTATGGAT ACAGCATGGC TATAGCCACA GAGCAAGCTG TACAAAAAGT TTTTCCTAAT 
AAGAGAAGCT TCATTCTTAC CCGCTCAACA TTTGCTGGAT CTGGAAGACA TGCTGCGCAT 
TGGTTAGGAG ACAATACTGC TTCATGGGAA CAAATGGAAT GGTCTATAAC TGGAATGCTG 
GAGTTCAGTT TGTTTGGAAT ACCTTTGGTT GGAGCAGACA TCTGTGGATT TGTGGCTGAA 
ACCACAGAAG AACTTTGCAG AAGATGGATG CAACTTGGGG CATTTTATCC ATTTTCCAGA 
AACCATAATT CTGACGGATA TGAACATCAG GATCCTGCAT TTTTTGGGCA GAATTCACTT 
TTGGTTAAAT CATCAAGGCA GTATTTAACT ATTCGCTACA CCTTATTACC CTTCCTCTAC 
ACTCTGTTTT ATAAAGCCCA TGTGTTTGGA GAAACAGTAG CAAGACCAGT TCTTCATGAG 
TTTTATGAGG ATACGAACAG CTGGATTGAG GACACTGAGT TTTTGTGGGG CCCTGCATTA 
CTTATTACTC CTGTTCTAAA ACAGGGAGCA GATACTGTGA GTGCCTACAT CCCTGATGCT 
ATTTGGTATG ATTATGAATC TGGTGCAAAA AGGCCATGGA GGAAACAACG GGTTGATATG 
TATCTTCCAG CAGACAAAAT AGGATTACAT CTTAGAGGAG GTTATATCAT CCCCATTCAA 
GAACCAGATG TAACAACAAC AGCAAGCCGT AAGAATCCTC TAGGACTTAT AGTCGCATTA 
GGTGAAAACA ACACAGCCAA AGGAGACTTT TTCTGGGATG ATGGAGAAAC TAAAGATACA 
ATACAAAATG GCAACTACAT ATTATATACA TTTTCAGTTT CTAATAACAC ATTAGATATT 
GTGTGCACAC ATTCATCATA TCAGGAAGGA ACTACCTTAG CATTTCAGAC TGTAAAAATC 
CTTGGGTTGA CAGACAGTGT TACAGAAGTT AGAGTGGCGG AAAATAATCA ACCAATGAAC 
GCTCATTCCA ATTTCACTTA TGATGCTTCT AACCAGGTTC TCCTAATTGC AGATCTCAAA 
CTTAATCTTG GAAGAAACTT TAGTGTTCAA TGGAATCAAA TTTTCTCAGA AAATGAAAGA 
TTTAATTGTT ATCCAGATGC AGATTTGGCA ACTGAACAAA AGTGCACACA ACGTGGCTGT 
GTATGGAGAA CGGGTTCTTC TCTATCCAAA GCACCTGAGT GTTACTTTCC CAGACAAGAT 
AACTCTTATT CAGTCAACTC AGCTCGCTAT TCATCCATGG GTATAACAGC TGACCTCCAA 
CTAAATACTG CAAATGCCAG AATAAAGTTA CCTTCTGACC CCATCTCAAC TCTTCGTGTG 
GAGGTGAAAT ATCACAAAAA TGATATGTTG CAGTTTAAGA TTTATGATCC CCAAAAGAAG 
AGATATGAAG TACCAGTACC GTTAAACATT CCAACCACCC CAATAAGTAC TTATGAAGAC 
AGACTTTATG ATGTGGAAAT CAAGGAAAAT CCTTTTGGCA TCCAGATTCG ACGGAGAAGC 
AGTGGAAGAG TCATTTGGGA TTCTTGGCTG CCTGGATTTG CTTTTAATGA CCAGTTCATT 
CAAATATCGA CTCGCCTGCC ATCAGAATAT ATATATGGTT TTGGGGAAGT GGAACATACA 
GCATTTAAGC GAGATCTGAA CTGGAATACT TGGGGAATGT TCACAAGAGA CCAACCCCCT 
GGTTACAAAC TTAATTCCTA TGGATTTCAT CCCTATTACA TGGCTCTGGA AGAGGAGGGC 
AATGCTCATG GTGTTTTCTT ACTCAACAGC AATGCAATGG ATGTTACATT CCAGCCAACT 
CCTGCTCTAA CTTACCGTAC AGTTGGAGGG ATCTTGGATT TTTATATGTT TTTGGGCCCA 
ACTCCAGAAG TTGCAACAAA GCAATACCAT GAAGTAATTG GCCATCCAGT CATGCCAGCT 
TATTGGGCTT TGGGATTCCA ATTATGTCGT TATGGATATG CAAATACTTC AGAGGTTCGG 
GAATTATATG ACGCTATGGT GGCTGCTAAC ATCCCCTATG ATGTTCAGTA CACAGACATT 
GACTACATGG AAAGGCAGCT AGACTTTACA ATTGGTGAAG CATTCCAGGA CCTTCCTCAG 
TTTGTTGACA AAATAAGAGG AGAAGGAATG AGATACATTA TTATCCTGGA TCCAGCAATT 
TCAGGAAATG AAACAAAGAC TTACCCTGCA TTTGAAAGAG GACAGCAGAA TGATGTCTTT 
GTCAAATGGC CAAACACCAA TGACATTTGT TGGGCAAAGG TTTGGCCAGA TTTGCCCAAC 
ATAACAATAG ATAAAACTCT AACGGAAGAT GAAGCTGTTA ATGCTTCCAG AGCTCATGTA 
GCTTTCCCAG ATTTCTTCAG GACTTCCACA GCAGAGTGGT GGGCCAGAGA AATTGTGGAC 
TTTTACAATG AAAAGATGAA GTTTGATGGT TTGTGGATTG ATATGAATGA GCCATCAAGT 
TTTGTAAATG GAACAACTAC TAATCAATGC AGAAATGACG AACTAAATTA TCCACCTTAT 
TTCCCAGAAC TCACAAAAAG AACTGATGGA TTACATTTCA GAACAATTTG CATGGAAGCT 
GAGCAGATTC TTAGTGATGG AACATCAGTT TTGCATTACG ATGTTCACAA TCTCTATGGA 
TGGTCACAGA TGAAACCTAC TCATGATGCA TTGCAGAAGA CAACTGGAAA AAGAGGGATT 
GTAATTTCTC GTTCCACGTA TCCTACTAGT GGACGATGGG GAGGACACTG GCTTGGAGAC 
AACTATGCAC GATGGGACAA CATGGACAAA TCAATCATTG GTATGATGGA ATTTAGTCTG 
TTTGGAATGT CATATACTGG AGCAGACATC TGTGGTTTTT TCAACAACTC AGAATATCAT 
CTCTGTACCC GCTGGATGCA ACTTGGAGCA TTTTATCCAT ACTCAAGGAA TCACAACATT 
GCAAATACTA GAAGACAAGA TCCCGCTTCC TGGAATGAAA CTTTTGCTGA AATGTCAAGG 
AATATTCTAA ATATTAGATA CACCTTATTG CCCTATTTTT ACACACAAAT GCATGAAATT 
CATGCTAATG GTGGCACTGT TATCCGACCC CTTTTGCATG AGTTCTTTGA TGAAAAACCA 
ACCTGGGATA TATTCAAGCA GTTCTTATGG GGTCCAGCAT TTATGGTTAC CCCAGTACTG 
GAACCTTATG TTCAAACTGT AAATGCCTAC GTCCCCAATG CTCGGTGGTT TGACTACCAT 
ACAGGCAAAG ATATTGGCGT CAGAGGACAA TTTCAAACAT TTAATGCTTC TTATGACACA 
ATAAACCTAC ATGTCCGTGG TGGTCACATC CTACCATGTC AAGAGCCAGC TCAAAACACA 
TTTTACAGTC GACAAAAACA CATGAAGCTC ATTGTTGCTG CAGATGATAA TCAGATGGCA 
CAGGGTTCTC TGTTTTGGGA TGATGGAGAG AGTATAGACA CCTATGAAAG AGACCTATAT 
TTATCTGTAC AATTTAATTT AAACCAGACC ACCTTAACAA GCACTATATT GAAGAGAGGT 
TACATAAATA AAAGTGAAAC GAGGCTTGGA TCCCTTCATG TATGGGGGAA AGGAACTACT 
CCTGTCAATG CAGTTACTCT AACGTATAAC GGAAATAAAA ATTCGCTTCC TTTTAATGAA 
GACACTACCA ACATGATATT ACGTATTGAT CTGACCACAC ACAATGTTAC TCTAGAAGAA 
CCAATAGAAA TCAACTGGTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032.2
CDS	64..5547
Translation	MARKKFSGLEISLIVLFVIVTIIAIALIVVLATKTPAVDEISDSTSTPATTRVTTNPSDSGKCPNVLNDPVNVRINCIPEQFPTEGICAQRGCCWRPWNDSLIPWCFFVDNHGYNVQDMTTTSIGVEAKLNRIPSPTLFGNDINSVLFTTQNQTPNRFRFKITDPNNRRYEVPHQYVKEFTGPTVSDTLYDVKVAQNPFSIQVIRKSNGKTLFDTSIGPLVYSDQYLQISTRLPSDYIYGIGEQVHKRFRHDLSWKTWPIFTRDQLPGDNNNNLYGHQTFFMCIEDTSGKSFGVFLMNSNAMEIFIQPTPIVTYRVTGGILDFYILLGDTPEQVVQQYQQLVGLPAMPAYWNLGFQLSRWNYKSLDVVKEVVRRNREAGIPFDTQVTDIDYMEDKKDFTYDQVAFNGLPQFVQDLHDHGQKYVIILDPAISIGRRANGTTYATYERGNTQHVWINESDGSTPIIGEVWPGLTVYPDFTNPNCIDWWANECSIFHQEVQYDGLWIDMNEVSSFIQGSTKGCNVNKLNYPPFTPDILDKLMYSKTICMDAVQNWGKQYDVHSLYGYSMAIATEQAVQKVFPNKRSFILTRSTFAGSGRHAAHWLGDNTASWEQMEWSITGMLEFSLFGIPLVGADICGFVAETTEELCRRWMQLGAFYPFSRNHNSDGYEHQDPAFFGQNSLLVKSSRQYLTIRYTLLPFLYTLFYKAHVFGETVARPVLHEFYEDTNSWIEDTEFLWGPALLITPVLKQGADTVSAYIPDAIWYDYESGAKRPWRKQRVDMYLPADKIGLHLRGGYIIPIQEPDVTTTASRKNPLGLIVALGENNTAKGDFFWDDGETKDTIQNGNYILYTFSVSNNTLDIVCTHSSYQEGTTLAFQTVKILGLTDSVTEVRVAENNQPMNAHSNFTYDASNQVLLIADLKLNLGRNFSVQWNQIFSENERFNCYPDADLATEQKCTQRGCVWRTGSSLSKAPECYFPRQDNSYSVNSARYSSMGITADLQLNTANARIKLPSDPISTLRVEVKYHKNDMLQFKIYDPQKKRYEVPVPLNIPTTPISTYEDRLYDVEIKENPFGIQIRRRSSGRVIWDSWLPGFAFNDQFIQISTRLPSEYIYGFGEVEHTAFKRDLNWNTWGMFTRDQPPGYKLNSYGFHPYYMALEEEGNAHGVFLLNSNAMDVTFQPTPALTYRTVGGILDFYMFLGPTPEVATKQYHEVIGHPVMPAYWALGFQLCRYGYANTSEVRELYDAMVAANIPYDVQYTDIDYMERQLDFTIGEAFQDLPQFVDKIRGEGMRYIIILDPAISGNETKTYPAFERGQQNDVFVKWPNTNDICWAKVWPDLPNITIDKTLTEDEAVNASRAHVAFPDFFRTSTAEWWAREIVDFYNEKMKFDGLWIDMNEPSSFVNGTTTNQCRNDELNYPPYFPELTKRTDGLHFRTICMEAEQILSDGTSVLHYDVHNLYGWSQMKPTHDALQKTTGKRGIVISRSTYPTSGRWGGHWLGDNYARWDNMDKSIIGMMEFSLFGMSYTGADICGFFNNSEYHLCTRWMQLGAFYPYSRNHNIANTRRQDPASWNETFAEMSRNILNIRYTLLPYFYTQMHEIHANGGTVIRPLLHEFFDEKPTWDIFKQFLWGPAFMVTPVLEPYVQTVNAYVPNARWFDYHTGKDIGVRGQFQTFNASYDTINLHVRGGHILPCQEPAQNTFYSRQKHMKLIVAADDNQMAQGSLFWDDGESIDTYERDLYLSVQFNLNQTTLTSTILKRGYINKSETRLGSLHVWGKGTTPVNAVTLTYNGNKNSLPFNEDTTNMILRIDLTTHNVTLEEPIEINWS

Target ORF information:

RefSeq Version	NM_001041.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sucrase-isomaltase (SI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001041.3

ORF Insert Sequence:

ATGGCAAGAA AGAAATTTAG TGGATTGGAA ATCTCTCTGA TTGTCCTTTT TGTCATAGTT 
ACTATAATAG CTATTGCCTT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGATGAA 
ATTAGTGATT CTACTTCAAC TCCAGCTACT ACTCGTGTGA CTACAAATCC TTCTGATTCA 
GGAAAATGTC CAAATGTGTT AAATGATCCT GTCAATGTGA GAATAAACTG CATTCCAGAA 
CAATTCCCAA CAGAGGGAAT TTGTGCACAG AGAGGCTGCT GCTGGAGGCC GTGGAATGAC 
TCTCTTATTC CTTGGTGCTT CTTCGTTGAT AATCATGGTT ATAACGTTCA AGACATGACA 
ACAACAAGTA TTGGAGTTGA AGCCAAATTA AACAGGATAC CTTCACCTAC ACTATTTGGA 
AATGACATCA ACAGTGTTCT CTTCACAACT CAAAATCAGA CACCCAATCG TTTCCGGTTC 
AAGATTACTG ATCCAAATAA TAGAAGATAT GAAGTTCCTC ATCAGTATGT AAAAGAGTTT 
ACTGGACCCA CAGTTTCTGA TACGTTGTAT GATGTGAAGG TTGCCCAAAA CCCATTTAGC 
ATCCAAGTTA TTAGGAAAAG CAACGGTAAA ACTTTGTTTG ACACCAGCAT TGGTCCCTTA 
GTGTACTCTG ACCAGTACTT ACAGATCTCA ACCCGTCTTC CAAGTGATTA TATTTATGGT 
ATTGGAGAAC AAGTTCATAA GAGATTTCGT CATGATTTAT CCTGGAAAAC ATGGCCAATT 
TTTACTCGAG ACCAACTTCC TGGTGATAAT AATAATAATT TATACGGCCA TCAAACATTC 
TTTATGTGTA TTGAAGATAC ATCTGGAAAG TCATTCGGTG TTTTTTTAAT GAATAGCAAT 
GCAATGGAGA TTTTTATCCA GCCTACTCCA ATAGTAACAT ATAGAGTTAC CGGTGGCATT 
CTGGATTTTT ACATCCTTCT AGGAGATACA CCAGAACAAG TAGTTCAACA GTATCAACAG 
CTTGTTGGAC TACCAGCAAT GCCAGCATAT TGGAATCTTG GATTCCAACT AAGTCGCTGG 
AATTATAAGT CACTAGATGT AGTGAAAGAA GTGGTAAGGA GAAACCGGGA AGCTGGCATA 
CCATTTGATA CACAGGTCAC TGATATTGAC TACATGGAAG ACAAGAAAGA CTTTACTTAT 
GATCAAGTTG CGTTTAACGG ACTCCCTCAA TTTGTGCAAG ATTTGCATGA CCATGGACAG 
AAATATGTCA TCATCTTGGA CCCTGCAATT TCCATAGGTC GACGTGCCAA TGGAACAACA 
TATGCAACCT ATGAGAGGGG AAACACACAA CATGTGTGGA TAAATGAGTC AGATGGAAGT 
ACACCAATTA TTGGAGAGGT ATGGCCAGGA TTAACAGTAT ACCCTGATTT CACTAACCCA 
AACTGCATTG ATTGGTGGGC AAATGAATGC AGTATTTTCC ATCAAGAAGT GCAATATGAT 
GGACTTTGGA TTGACATGAA TGAAGTTTCC AGCTTTATTC AAGGTTCAAC AAAAGGATGT 
AATGTAAACA AATTGAATTA TCCACCGTTT ACTCCTGATA TTCTTGACAA ACTCATGTAT 
TCCAAAACAA TTTGCATGGA TGCTGTGCAG AACTGGGGTA AACAGTATGA TGTTCATAGC 
CTCTATGGAT ACAGCATGGC TATAGCCACA GAGCAAGCTG TACAAAAAGT TTTTCCTAAT 
AAGAGAAGCT TCATTCTTAC CCGCTCAACA TTTGCTGGAT CTGGAAGACA TGCTGCGCAT 
TGGTTAGGAG ACAATACTGC TTCATGGGAA CAAATGGAAT GGTCTATAAC TGGAATGCTG 
GAGTTCAGTT TGTTTGGAAT ACCTTTGGTT GGAGCAGACA TCTGTGGATT TGTGGCTGAA 
ACCACAGAAG AACTTTGCAG AAGATGGATG CAACTTGGGG CATTTTATCC ATTTTCCAGA 
AACCATAATT CTGACGGATA TGAACATCAG GATCCTGCAT TTTTTGGGCA GAATTCACTT 
TTGGTTAAAT CATCAAGGCA GTATTTAACT ATTCGCTACA CCTTATTACC CTTCCTCTAC 
ACTCTGTTTT ATAAAGCCCA TGTGTTTGGA GAAACAGTAG CAAGACCAGT TCTTCATGAG 
TTTTATGAGG ATACGAACAG CTGGATTGAG GACACTGAGT TTTTGTGGGG CCCTGCATTA 
CTTATTACTC CTGTTCTAAA ACAGGGAGCA GATACTGTGA GTGCCTACAT CCCTGATGCT 
ATTTGGTATG ATTATGAATC TGGTGCAAAA AGGCCATGGA GGAAACAACG GGTTGATATG 
TATCTTCCAG CAGACAAAAT AGGATTACAT CTTAGAGGAG GTTATATCAT CCCCATTCAA 
GAACCAGATG TAACAACAAC AGCAAGCCGT AAGAATCCTC TAGGACTTAT AGTCGCATTA 
GGTGAAAACA ACACAGCCAA AGGAGACTTT TTCTGGGATG ATGGAGAAAC TAAAGATACA 
ATACAAAATG GCAACTACAT ATTATATACA TTTTCAGTTT CTAATAACAC ATTAGATATT 
GTGTGCACAC ATTCATCATA TCAGGAAGGA ACTACCTTAG CATTTCAGAC TGTAAAAATC 
CTTGGGTTGA CAGACAGTGT TACAGAAGTT AGAGTGGCGG AAAATAATCA ACCAATGAAC 
GCTCATTCCA ATTTCACTTA TGATGCTTCT AACCAGGTTC TCCTAATTGC AGATCTCAAA 
CTTAATCTTG GAAGAAACTT TAGTGTTCAA TGGAATCAAA TTTTCTCAGA AAATGAAAGA 
TTTAATTGTT ATCCAGATGC AGATTTGGCA ACTGAACAAA AGTGCACACA ACGTGGCTGT 
GTATGGAGAA CGGGTTCTTC TCTATCCAAA GCACCTGAGT GTTACTTTCC CAGACAAGAT 
AACTCTTATT CAGTCAACTC AGCTCGCTAT TCATCCATGG GTATAACAGC TGACCTCCAA 
CTAAATACTG CAAATGCCAG AATAAAGTTA CCTTCTGACC CCATCTCAAC TCTTCGTGTG 
GAGGTGAAAT ATCACAAAAA TGATATGTTG CAGTTTAAGA TTTATGATCC CCAAAAGAAG 
AGATATGAAG TACCAGTACC GTTAAACATT CCAACCACCC CAATAAGTAC TTATGAAGAC 
AGACTTTATG ATGTGGAAAT CAAGGAAAAT CCTTTTGGCA TCCAGATTCG ACGGAGAAGC 
AGTGGAAGAG TCATTTGGGA TTCTTGGCTG CCTGGATTTG CTTTTAATGA CCAGTTCATT 
CAAATATCGA CTCGCCTGCC ATCAGAATAT ATATATGGTT TTGGGGAAGT GGAACATACA 
GCATTTAAGC GAGATCTGAA CTGGAATACT TGGGGAATGT TCACAAGAGA CCAACCCCCT 
GGTTACAAAC TTAATTCCTA TGGATTTCAT CCCTATTACA TGGCTCTGGA AGAGGAGGGC 
AATGCTCATG GTGTTTTCTT ACTCAACAGC AATGCAATGG ATGTTACATT CCAGCCAACT 
CCTGCTCTAA CTTACCGTAC AGTTGGAGGG ATCTTGGATT TTTATATGTT TTTGGGCCCA 
ACTCCAGAAG TTGCAACAAA GCAATACCAT GAAGTAATTG GCCATCCAGT CATGCCAGCT 
TATTGGGCTT TGGGATTCCA ATTATGTCGT TATGGATATG CAAATACTTC AGAGGTTCGG 
GAATTATATG ACGCTATGGT GGCTGCTAAC ATCCCCTATG ATGTTCAGTA CACAGACATT 
GACTACATGG AAAGGCAGCT AGACTTTACA ATTGGTGAAG CATTCCAGGA CCTTCCTCAG 
TTTGTTGACA AAATAAGAGG AGAAGGAATG AGATACATTA TTATCCTGGA TCCAGCAATT 
TCAGGAAATG AAACAAAGAC TTACCCTGCA TTTGAAAGAG GACAGCAGAA TGATGTCTTT 
GTCAAATGGC CAAACACCAA TGACATTTGT TGGGCAAAGG TTTGGCCAGA TTTGCCCAAC 
ATAACAATAG ATAAAACTCT AACGGAAGAT GAAGCTGTTA ATGCTTCCAG AGCTCATGTA 
GCTTTCCCAG ATTTCTTCAG GACTTCCACA GCAGAGTGGT GGGCCAGAGA AATTGTGGAC 
TTTTACAATG AAAAGATGAA GTTTGATGGT TTGTGGATTG ATATGAATGA GCCATCAAGT 
TTTGTAAATG GAACAACTAC TAATCAATGC AGAAATGACG AACTAAATTA TCCACCTTAT 
TTCCCAGAAC TCACAAAAAG AACTGATGGA TTACATTTCA GAACAATTTG CATGGAAGCT 
GAGCAGATTC TTAGTGATGG AACATCAGTT TTGCATTACG ATGTTCACAA TCTCTATGGA 
TGGTCACAGA TGAAACCTAC TCATGATGCA TTGCAGAAGA CAACTGGAAA AAGAGGGATT 
GTAATTTCTC GTTCCACGTA TCCTACTAGT GGACGATGGG GAGGACACTG GCTTGGAGAC 
AACTATGCAC GATGGGACAA CATGGACAAA TCAATCATTG GTATGATGGA ATTTAGTCTG 
TTTGGAATGT CATATACTGG AGCAGACATC TGTGGTTTTT TCAACAACTC AGAATATCAT 
CTCTGTACCC GCTGGATGCA ACTTGGAGCA TTTTATCCAT ACTCAAGGAA TCACAACATT 
GCAAATACTA GAAGACAAGA TCCCGCTTCC TGGAATGAAA CTTTTGCTGA AATGTCAAGG 
AATATTCTAA ATATTAGATA CACCTTATTG CCCTATTTTT ACACACAAAT GCATGAAATT 
CATGCTAATG GTGGCACTGT TATCCGACCC CTTTTGCATG AGTTCTTTGA TGAAAAACCA 
ACCTGGGATA TATTCAAGCA GTTCTTATGG GGTCCAGCAT TTATGGTTAC CCCAGTACTG 
GAACCTTATG TTCAAACTGT AAATGCCTAC GTCCCCAATG CTCGGTGGTT TGACTACCAT 
ACAGGCAAAG ATATTGGCGT CAGAGGACAA TTTCAAACAT TTAATGCTTC TTATGACACA 
ATAAACCTAC ATGTCCGTGG TGGTCACATC CTACCATGTC AAGAGCCAGC TCAAAACACA 
TTTTACAGTC GACAAAAACA CATGAAGCTC ATTGTTGCTG CAGATGATAA TCAGATGGCA 
CAGGGTTCTC TGTTTTGGGA TGATGGAGAG AGTATAGACA CCTATGAAAG AGACCTATAT 
TTATCTGTAC AATTTAATTT AAACCAGACC ACCTTAACAA GCACTATATT GAAGAGAGGT 
TACATAAATA AAAGTGAAAC GAGGCTTGGA TCCCTTCATG TATGGGGGAA AGGAACTACT 
CCTGTCAATG CAGTTACTCT AACGTATAAC GGAAATAAAA ATTCGCTTCC TTTTAATGAA 
GACACTACCA ACATGATATT ACGTATTGAT CTGACCACAC ACAATGTTAC TCTAGAAGAA 
CCAATAGAAA TCAACTGGTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI ( XM_011513078.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511380.1 Homo sapiens sucrase-isomaltase (SI), transcript variant X1, mRNA.

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CloneID	OHu60497
Clone ID Related Accession (Same CDS sequence)	XM_011513078.2
Accession Version	XM_011513078.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sucrase-isomaltase, intestinal isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513078.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTTC CTCCCCCAGA AATTAGTGAT TCTACTTCAA CTCCAGCTAC TACTCGTGTG 
ACTACAAATC CTTCTGATTC AGGAAAATGT CCAAATGTGT TAAATGATCC TGTCAATGTG 
AGAATAAACT GCATTCCAGA ACAATTCCCA ACAGAGGGAA TTTGTGCACA GAGAGGCTGC 
TGCTGGAGGC CGTGGAATGA CTCTCTTATT CCTTGGTGCT TCTTCGTTGA TAATCATGGT 
TATAACGTTC AAGACATGAC AACAACAAGT ATTGGAGTTG AAGCCAAATT AAACAGGATA 
CCTTCACCTA CACTATTTGG AAATGACATC AACAGTGTTC TCTTCACAAC TCAAAATCAG 
ACACCCAATC GTTTCCGGTT CAAGATTACT GATCCAAATA ATAGAAGATA TGAAGTTCCT 
CATCAGTATG TAAAAGAGTT TACTGGACCC ACAGTTTCTG ATACGTTGTA TGATGTGAAG 
GTTGCCCAAA ACCCATTTAG CATCCAAGTT ATTAGGAAAA GCAACGGTAA AACTTTGTTT 
GACACCAGCA TTGGTCCCTT AGTGTACTCT GACCAGTACT TACAGATCTC AACCCGTCTT 
CCAAGTGATT ATATTTATGG TATTGGAGAA CAAGTTCATA AGAGATTTCG TCATGATTTA 
TCCTGGAAAA CATGGCCAAT TTTTACTCGA GACCAACTTC CTGGTGATAA TAATAATAAT 
TTATACGGCC ATCAAACATT CTTTATGTGT ATTGAAGATA CATCTGGAAA GTCATTCGGT 
GTTTTTTTAA TGAATAGCAA TGCAATGGAG ATTTTTATCC AGCCTACTCC AATAGTAACA 
TATAGAGTTA CCGGTGGCAT TCTGGATTTT TACATCCTTC TAGGAGATAC ACCAGAACAA 
GTAGTTCAAC AGTATCAACA GCTTGTTGGA CTACCAGCAA TGCCAGCATA TTGGAATCTT 
GGATTCCAAC TAAGTCGCTG GAATTATAAG TCACTAGATG TAGTGAAAGA AGTGGTAAGG 
AGAAACCGGG AAGCTGGCAT ACCATTTGAT ACACAGGTCA CTGATATTGA CTACATGGAA 
GACAAGAAAG ACTTTACTTA TGATCAAGTT GCGTTTAACG GACTCCCTCA ATTTGTGCAA 
GATTTGCATG ACCATGGACA GAAATATGTC ATCATCTTGG ACCCTGCAAT TTCCATAGGT 
CGACGTGCCA ATGGAACAAC ATATGCAACC TATGAGAGGG GAAACACACA ACATGTGTGG 
ATAAATGAGT CAGATGGAAG TACACCAATT ATTGGAGAGG TATGGCCAGG ATTAACAGTA 
TACCCTGATT TCACTAACCC AAACTGCATT GATTGGTGGG CAAATGAATG CAGTATTTTC 
CATCAAGAAG TGCAATATGA TGGACTTTGG ATTGACATGA ATGAAGTTTC CAGCTTTATT 
CAAGGTTCAA CAAAAGGATG TAATGTAAAC AAATTGAATT ATCCACCGTT TACTCCTGAT 
ATTCTTGACA AACTCATGTA TTCCAAAACA ATTTGCATGG ATGCTGTGCA GAACTGGGGT 
AAACAGTATG ATGTTCATAG CCTCTATGGA TACAGCATGG CTATAGCCAC AGAGCAAGCT 
GTACAAAAAG TTTTTCCTAA TAAGAGAAGC TTCATTCTTA CCCGCTCAAC ATTTGCTGGA 
TCTGGAAGAC ATGCTGCGCA TTGGTTAGGA GACAATACTG CTTCATGGGA ACAAATGGAA 
TGGTCTATAA CTGGAATGCT GGAGTTCAGT TTGTTTGGAA TACCTTTGGT TGGAGCAGAC 
ATCTGTGGAT TTGTGGCTGA AACCACAGAA GAACTTTGCA GAAGATGGAT GCAACTTGGG 
GCATTTTATC CATTTTCCAG AAACCATAAT TCTGACGGAT ATGAACATCA GGATCCTGCA 
TTTTTTGGGC AGAATTCACT TTTGGTTAAA TCATCAAGGC AGTATTTAAC TATTCGCTAC 
ACCTTATTAC CCTTCCTCTA CACTCTGTTT TATAAAGCCC ATGTGTTTGG AGAAACAGTA 
GCAAGACCAG TTCTTCATGA GTTTTATGAG GATACGAACA GCTGGATTGA GGACACTGAG 
TTTTTGTGGG GCCCTGCATT ACTTATTACT CCTGTTCTAA AACAGGGAGC AGATACTGTG 
AGTGCCTACA TCCCTGATGC TATTTGGTAT GATTATGAAT CTGGTGCAAA AAGGCCATGG 
AGGAAACAAC GGGTTGATAT GTATCTTCCA GCAGACAAAA TAGGATTACA TCTTAGAGGA 
GGTTATATCA TCCCCATTCA AGAACCAGAT GTAACAACAA CAGCAAGCCG TAAGAATCCT 
CTAGGACTTA TAGTCGCATT AGGTGAAAAC AACACAGCCA AAGGAGACTT TTTCTGGGAT 
GATGGAGAAA CTAAAGATAC AATACAAAAT GGCAACTACA TATTATATAC ATTTTCAGTT 
TCTAATAACA CATTAGATAT TGTGTGCACA CATTCATCAT ATCAGGAAGG AACTACCTTA 
GCATTTCAGA CTGTAAAAAT CCTTGGGTTG ACAGACAGTG TTACAGAAGT TAGAGTGGCG 
GAAAATAATC AACCAATGAA CGCTCATTCC AATTTCACTT ATGATGCTTC TAACCAGGTT 
CTCCTAATTG CAGATCTCAA ACTTAATCTT GGAAGAAACT TTAGTGTTCA ATGGAATCAA 
ATTTTCTCAG AAAATGAAAG ATTTAATTGT TATCCAGATG CAGATTTGGC AACTGAACAA 
AAGTGCACAC AACGTGGCTG TGTATGGAGA ACGGGTTCTT CTCTATCCAA AGCACCTGAG 
TGTTACTTTC CCAGACAAGA TAACTCTTAT TCAGTCAACT CAGCTCGCTA TTCATCCATG 
GGTATAACAG CTGACCTCCA ACTAAATACT GCAAATGCCA GAATAAAGTT ACCTTCTGAC 
CCCATCTCAA CTCTTCGTGT GGAGGTGAAA TATCACAAAA ATGATATGTT GCAGTTTAAG 
ATTTATGATC CCCAAAAGAA GAGATATGAA GTACCAGTAC CGTTAAACAT TCCAACCACC 
CCAATAAGTA CTTATGAAGA CAGACTTTAT GATGTGGAAA TCAAGGAAAA TCCTTTTGGC 
ATCCAGATTC GACGGAGAAG CAGTGGAAGA GTCATTTGGG ATTCTTGGCT GCCTGGATTT 
GCTTTTAATG ACCAGTTCAT TCAAATATCG ACTCGCCTGC CATCAGAATA TATATATGGT 
TTTGGGGAAG TGGAACATAC AGCATTTAAG CGAGATCTGA ACTGGAATAC TTGGGGAATG 
TTCACAAGAG ACCAACCCCC TGGTTACAAA CTTAATTCCT ATGGATTTCA TCCCTATTAC 
ATGGCTCTGG AAGAGGAGGG CAATGCTCAT GGTGTTTTCT TACTCAACAG CAATGCAATG 
GATGTTACAT TCCAGCCAAC TCCTGCTCTA ACTTACCGTA CAGTTGGAGG GATCTTGGAT 
TTTTATATGT TTTTGGGCCC AACTCCAGAA GTTGCAACAA AGCAATACCA TGAAGTAATT 
GGCCATCCAG TCATGCCAGC TTATTGGGCT TTGGGATTCC AATTATGTCG TTATGGATAT 
GCAAATACTT CAGAGGTTCG GGAATTATAT GACGCTATGG TGGCTGCTAA CATCCCCTAT 
GATGTTCAGT ACACAGACAT TGACTACATG GAAAGGCAGC TAGACTTTAC AATTGGTGAA 
GCATTCCAGG ACCTTCCTCA GTTTGTTGAC AAAATAAGAG GAGAAGGAAT GAGATACATT 
ATTATCCTGG ATCCAGCAAT TTCAGGAAAT GAAACAAAGA CTTACCCTGC ATTTGAAAGA 
GGACAGCAGA ATGATGTCTT TGTCAAATGG CCAAACACCA ATGACATTTG TTGGGCAAAG 
GTTTGGCCAG ATTTGCCCAA CATAACAATA GATAAAACTC TAACGGAAGA TGAAGCTGTT 
AATGCTTCCA GAGCTCATGT AGCTTTCCCA GATTTCTTCA GGACTTCCAC AGCAGAGTGG 
TGGGCCAGAG AAATTGTGGA CTTTTACAAT GAAAAGATGA AGTTTGATGG TTTGTGGATT 
GATATGAATG AGCCATCAAG TTTTGTAAAT GGAACAACTA CTAATCAATG CAGAAATGAC 
GAACTAAATT ATCCACCTTA TTTCCCAGAA CTCACAAAAA GAACTGATGG ATTACATTTC 
AGAACAATTT GCATGGAAGC TGAGCAGATT CTTAGTGATG GAACATCAGT TTTGCATTAC 
GATGTTCACA ATCTCTATGG ATGGTCACAG ATGAAACCTA CTCATGATGC ATTGCAGAAG 
ACAACTGGAA AAAGAGGGAT TGTAATTTCT CGTTCCACGT ATCCTACTAG TGGACGATGG 
GGAGGACACT GGCTTGGAGA CAACTATGCA CGATGGGACA ACATGGACAA ATCAATCATT 
GGTATGATGG AATTTAGTCT GTTTGGAATG TCATATACTG GAGCAGACAT CTGTGGTTTT 
TTCAACAACT CAGAATATCA TCTCTGTACC CGCTGGATGC AACTTGGAGC ATTTTATCCA 
TACTCAAGGA ATCACAACAT TGCAAATACT AGAAGACAAG ATCCCGCTTC CTGGAATGAA 
ACTTTTGCTG AAATGTCAAG GAATATTCTA AATATTAGAT ACACCTTATT GCCCTATTTT 
TACACACAAA TGCATGAAAT TCATGCTAAT GGTGGCACTG TTATCCGACC CCTTTTGCAT 
GAGTTCTTTG ATGAAAAACC AACCTGGGAT ATATTCAAGC AGTTCTTATG GGGTCCAGCA 
TTTATGGTTA CCCCAGTACT GGAACCTTAT GTTCAAACTG TAAATGCCTA CGTCCCCAAT 
GCTCGGTGGT TTGACTACCA TACAGGCAAA GATATTGGCG TCAGAGGACA ATTTCAAACA 
TTTAATGCTT CTTATGACAC AATAAACCTA CATGTCCGTG GTGGTCACAT CCTACCATGT 
CAAGAGCCAG CTCAAAACAC ATTTTACAGT CGACAAAAAC ACATGAAGCT CATTGTTGCT 
GCAGATGATA ATCAGATGGC ACAGGGTTCT CTGTTTTGGG ATGATGGAGA GAGTATAGAC 
ACCTATGAAA GAGACCTATA TTTATCTGTA CAATTTAATT TAAACCAGAC CACCTTAACA 
AGCACTATAT TGAAGAGAGG TTACATAAAT AAAAGTGAAA CGAGGCTTGG ATCCCTTCAT 
GTATGGGGGA AAGGAACTAC TCCTGTCAAT GCAGTTACTC TAACGTATAA CGGAAATAAA 
AATTCGCTTC CTTTTAATGA AGACACTACC AACATGATAT TACGTATTGA TCTGACCACA 
CACAATGTTA CTCTAGAAGA ACCAATAGAA ATCAACTGGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511380.1
CDS	167..5551
Translation	MFFPPPEISDSTSTPATTRVTTNPSDSGKCPNVLNDPVNVRINCIPEQFPTEGICAQRGCCWRPWNDSLIPWCFFVDNHGYNVQDMTTTSIGVEAKLNRIPSPTLFGNDINSVLFTTQNQTPNRFRFKITDPNNRRYEVPHQYVKEFTGPTVSDTLYDVKVAQNPFSIQVIRKSNGKTLFDTSIGPLVYSDQYLQISTRLPSDYIYGIGEQVHKRFRHDLSWKTWPIFTRDQLPGDNNNNLYGHQTFFMCIEDTSGKSFGVFLMNSNAMEIFIQPTPIVTYRVTGGILDFYILLGDTPEQVVQQYQQLVGLPAMPAYWNLGFQLSRWNYKSLDVVKEVVRRNREAGIPFDTQVTDIDYMEDKKDFTYDQVAFNGLPQFVQDLHDHGQKYVIILDPAISIGRRANGTTYATYERGNTQHVWINESDGSTPIIGEVWPGLTVYPDFTNPNCIDWWANECSIFHQEVQYDGLWIDMNEVSSFIQGSTKGCNVNKLNYPPFTPDILDKLMYSKTICMDAVQNWGKQYDVHSLYGYSMAIATEQAVQKVFPNKRSFILTRSTFAGSGRHAAHWLGDNTASWEQMEWSITGMLEFSLFGIPLVGADICGFVAETTEELCRRWMQLGAFYPFSRNHNSDGYEHQDPAFFGQNSLLVKSSRQYLTIRYTLLPFLYTLFYKAHVFGETVARPVLHEFYEDTNSWIEDTEFLWGPALLITPVLKQGADTVSAYIPDAIWYDYESGAKRPWRKQRVDMYLPADKIGLHLRGGYIIPIQEPDVTTTASRKNPLGLIVALGENNTAKGDFFWDDGETKDTIQNGNYILYTFSVSNNTLDIVCTHSSYQEGTTLAFQTVKILGLTDSVTEVRVAENNQPMNAHSNFTYDASNQVLLIADLKLNLGRNFSVQWNQIFSENERFNCYPDADLATEQKCTQRGCVWRTGSSLSKAPECYFPRQDNSYSVNSARYSSMGITADLQLNTANARIKLPSDPISTLRVEVKYHKNDMLQFKIYDPQKKRYEVPVPLNIPTTPISTYEDRLYDVEIKENPFGIQIRRRSSGRVIWDSWLPGFAFNDQFIQISTRLPSEYIYGFGEVEHTAFKRDLNWNTWGMFTRDQPPGYKLNSYGFHPYYMALEEEGNAHGVFLLNSNAMDVTFQPTPALTYRTVGGILDFYMFLGPTPEVATKQYHEVIGHPVMPAYWALGFQLCRYGYANTSEVRELYDAMVAANIPYDVQYTDIDYMERQLDFTIGEAFQDLPQFVDKIRGEGMRYIIILDPAISGNETKTYPAFERGQQNDVFVKWPNTNDICWAKVWPDLPNITIDKTLTEDEAVNASRAHVAFPDFFRTSTAEWWAREIVDFYNEKMKFDGLWIDMNEPSSFVNGTTTNQCRNDELNYPPYFPELTKRTDGLHFRTICMEAEQILSDGTSVLHYDVHNLYGWSQMKPTHDALQKTTGKRGIVISRSTYPTSGRWGGHWLGDNYARWDNMDKSIIGMMEFSLFGMSYTGADICGFFNNSEYHLCTRWMQLGAFYPYSRNHNIANTRRQDPASWNETFAEMSRNILNIRYTLLPYFYTQMHEIHANGGTVIRPLLHEFFDEKPTWDIFKQFLWGPAFMVTPVLEPYVQTVNAYVPNARWFDYHTGKDIGVRGQFQTFNASYDTINLHVRGGHILPCQEPAQNTFYSRQKHMKLIVAADDNQMAQGSLFWDDGESIDTYERDLYLSVQFNLNQTTLTSTILKRGYINKSETRLGSLHVWGKGTTPVNAVTLTYNGNKNSLPFNEDTTNMILRIDLTTHNVTLEEPIEINWS

Target ORF information:

RefSeq Version	XM_011513078.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sucrase-isomaltase (SI), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513078.2

ORF Insert Sequence:

ATGTTTTTTC CTCCCCCAGA AATTAGTGAT TCTACTTCAA CTCCAGCTAC TACTCGTGTG 
ACTACAAATC CTTCTGATTC AGGAAAATGT CCAAATGTGT TAAATGATCC TGTCAATGTG 
AGAATAAACT GCATTCCAGA ACAATTCCCA ACAGAGGGAA TTTGTGCACA GAGAGGCTGC 
TGCTGGAGGC CGTGGAATGA CTCTCTTATT CCTTGGTGCT TCTTCGTTGA TAATCATGGT 
TATAACGTTC AAGACATGAC AACAACAAGT ATTGGAGTTG AAGCCAAATT AAACAGGATA 
CCTTCACCTA CACTATTTGG AAATGACATC AACAGTGTTC TCTTCACAAC TCAAAATCAG 
ACACCCAATC GTTTCCGGTT CAAGATTACT GATCCAAATA ATAGAAGATA TGAAGTTCCT 
CATCAGTATG TAAAAGAGTT TACTGGACCC ACAGTTTCTG ATACGTTGTA TGATGTGAAG 
GTTGCCCAAA ACCCATTTAG CATCCAAGTT ATTAGGAAAA GCAACGGTAA AACTTTGTTT 
GACACCAGCA TTGGTCCCTT AGTGTACTCT GACCAGTACT TACAGATCTC AACCCGTCTT 
CCAAGTGATT ATATTTATGG TATTGGAGAA CAAGTTCATA AGAGATTTCG TCATGATTTA 
TCCTGGAAAA CATGGCCAAT TTTTACTCGA GACCAACTTC CTGGTGATAA TAATAATAAT 
TTATACGGCC ATCAAACATT CTTTATGTGT ATTGAAGATA CATCTGGAAA GTCATTCGGT 
GTTTTTTTAA TGAATAGCAA TGCAATGGAG ATTTTTATCC AGCCTACTCC AATAGTAACA 
TATAGAGTTA CCGGTGGCAT TCTGGATTTT TACATCCTTC TAGGAGATAC ACCAGAACAA 
GTAGTTCAAC AGTATCAACA GCTTGTTGGA CTACCAGCAA TGCCAGCATA TTGGAATCTT 
GGATTCCAAC TAAGTCGCTG GAATTATAAG TCACTAGATG TAGTGAAAGA AGTGGTAAGG 
AGAAACCGGG AAGCTGGCAT ACCATTTGAT ACACAGGTCA CTGATATTGA CTACATGGAA 
GACAAGAAAG ACTTTACTTA TGATCAAGTT GCGTTTAACG GACTCCCTCA ATTTGTGCAA 
GATTTGCATG ACCATGGACA GAAATATGTC ATCATCTTGG ACCCTGCAAT TTCCATAGGT 
CGACGTGCCA ATGGAACAAC ATATGCAACC TATGAGAGGG GAAACACACA ACATGTGTGG 
ATAAATGAGT CAGATGGAAG TACACCAATT ATTGGAGAGG TATGGCCAGG ATTAACAGTA 
TACCCTGATT TCACTAACCC AAACTGCATT GATTGGTGGG CAAATGAATG CAGTATTTTC 
CATCAAGAAG TGCAATATGA TGGACTTTGG ATTGACATGA ATGAAGTTTC CAGCTTTATT 
CAAGGTTCAA CAAAAGGATG TAATGTAAAC AAATTGAATT ATCCACCGTT TACTCCTGAT 
ATTCTTGACA AACTCATGTA TTCCAAAACA ATTTGCATGG ATGCTGTGCA GAACTGGGGT 
AAACAGTATG ATGTTCATAG CCTCTATGGA TACAGCATGG CTATAGCCAC AGAGCAAGCT 
GTACAAAAAG TTTTTCCTAA TAAGAGAAGC TTCATTCTTA CCCGCTCAAC ATTTGCTGGA 
TCTGGAAGAC ATGCTGCGCA TTGGTTAGGA GACAATACTG CTTCATGGGA ACAAATGGAA 
TGGTCTATAA CTGGAATGCT GGAGTTCAGT TTGTTTGGAA TACCTTTGGT TGGAGCAGAC 
ATCTGTGGAT TTGTGGCTGA AACCACAGAA GAACTTTGCA GAAGATGGAT GCAACTTGGG 
GCATTTTATC CATTTTCCAG AAACCATAAT TCTGACGGAT ATGAACATCA GGATCCTGCA 
TTTTTTGGGC AGAATTCACT TTTGGTTAAA TCATCAAGGC AGTATTTAAC TATTCGCTAC 
ACCTTATTAC CCTTCCTCTA CACTCTGTTT TATAAAGCCC ATGTGTTTGG AGAAACAGTA 
GCAAGACCAG TTCTTCATGA GTTTTATGAG GATACGAACA GCTGGATTGA GGACACTGAG 
TTTTTGTGGG GCCCTGCATT ACTTATTACT CCTGTTCTAA AACAGGGAGC AGATACTGTG 
AGTGCCTACA TCCCTGATGC TATTTGGTAT GATTATGAAT CTGGTGCAAA AAGGCCATGG 
AGGAAACAAC GGGTTGATAT GTATCTTCCA GCAGACAAAA TAGGATTACA TCTTAGAGGA 
GGTTATATCA TCCCCATTCA AGAACCAGAT GTAACAACAA CAGCAAGCCG TAAGAATCCT 
CTAGGACTTA TAGTCGCATT AGGTGAAAAC AACACAGCCA AAGGAGACTT TTTCTGGGAT 
GATGGAGAAA CTAAAGATAC AATACAAAAT GGCAACTACA TATTATATAC ATTTTCAGTT 
TCTAATAACA CATTAGATAT TGTGTGCACA CATTCATCAT ATCAGGAAGG AACTACCTTA 
GCATTTCAGA CTGTAAAAAT CCTTGGGTTG ACAGACAGTG TTACAGAAGT TAGAGTGGCG 
GAAAATAATC AACCAATGAA CGCTCATTCC AATTTCACTT ATGATGCTTC TAACCAGGTT 
CTCCTAATTG CAGATCTCAA ACTTAATCTT GGAAGAAACT TTAGTGTTCA ATGGAATCAA 
ATTTTCTCAG AAAATGAAAG ATTTAATTGT TATCCAGATG CAGATTTGGC AACTGAACAA 
AAGTGCACAC AACGTGGCTG TGTATGGAGA ACGGGTTCTT CTCTATCCAA AGCACCTGAG 
TGTTACTTTC CCAGACAAGA TAACTCTTAT TCAGTCAACT CAGCTCGCTA TTCATCCATG 
GGTATAACAG CTGACCTCCA ACTAAATACT GCAAATGCCA GAATAAAGTT ACCTTCTGAC 
CCCATCTCAA CTCTTCGTGT GGAGGTGAAA TATCACAAAA ATGATATGTT GCAGTTTAAG 
ATTTATGATC CCCAAAAGAA GAGATATGAA GTACCAGTAC CGTTAAACAT TCCAACCACC 
CCAATAAGTA CTTATGAAGA CAGACTTTAT GATGTGGAAA TCAAGGAAAA TCCTTTTGGC 
ATCCAGATTC GACGGAGAAG CAGTGGAAGA GTCATTTGGG ATTCTTGGCT GCCTGGATTT 
GCTTTTAATG ACCAGTTCAT TCAAATATCG ACTCGCCTGC CATCAGAATA TATATATGGT 
TTTGGGGAAG TGGAACATAC AGCATTTAAG CGAGATCTGA ACTGGAATAC TTGGGGAATG 
TTCACAAGAG ACCAACCCCC TGGTTACAAA CTTAATTCCT ATGGATTTCA TCCCTATTAC 
ATGGCTCTGG AAGAGGAGGG CAATGCTCAT GGTGTTTTCT TACTCAACAG CAATGCAATG 
GATGTTACAT TCCAGCCAAC TCCTGCTCTA ACTTACCGTA CAGTTGGAGG GATCTTGGAT 
TTTTATATGT TTTTGGGCCC AACTCCAGAA GTTGCAACAA AGCAATACCA TGAAGTAATT 
GGCCATCCAG TCATGCCAGC TTATTGGGCT TTGGGATTCC AATTATGTCG TTATGGATAT 
GCAAATACTT CAGAGGTTCG GGAATTATAT GACGCTATGG TGGCTGCTAA CATCCCCTAT 
GATGTTCAGT ACACAGACAT TGACTACATG GAAAGGCAGC TAGACTTTAC AATTGGTGAA 
GCATTCCAGG ACCTTCCTCA GTTTGTTGAC AAAATAAGAG GAGAAGGAAT GAGATACATT 
ATTATCCTGG ATCCAGCAAT TTCAGGAAAT GAAACAAAGA CTTACCCTGC ATTTGAAAGA 
GGACAGCAGA ATGATGTCTT TGTCAAATGG CCAAACACCA ATGACATTTG TTGGGCAAAG 
GTTTGGCCAG ATTTGCCCAA CATAACAATA GATAAAACTC TAACGGAAGA TGAAGCTGTT 
AATGCTTCCA GAGCTCATGT AGCTTTCCCA GATTTCTTCA GGACTTCCAC AGCAGAGTGG 
TGGGCCAGAG AAATTGTGGA CTTTTACAAT GAAAAGATGA AGTTTGATGG TTTGTGGATT 
GATATGAATG AGCCATCAAG TTTTGTAAAT GGAACAACTA CTAATCAATG CAGAAATGAC 
GAACTAAATT ATCCACCTTA TTTCCCAGAA CTCACAAAAA GAACTGATGG ATTACATTTC 
AGAACAATTT GCATGGAAGC TGAGCAGATT CTTAGTGATG GAACATCAGT TTTGCATTAC 
GATGTTCACA ATCTCTATGG ATGGTCACAG ATGAAACCTA CTCATGATGC ATTGCAGAAG 
ACAACTGGAA AAAGAGGGAT TGTAATTTCT CGTTCCACGT ATCCTACTAG TGGACGATGG 
GGAGGACACT GGCTTGGAGA CAACTATGCA CGATGGGACA ACATGGACAA ATCAATCATT 
GGTATGATGG AATTTAGTCT GTTTGGAATG TCATATACTG GAGCAGACAT CTGTGGTTTT 
TTCAACAACT CAGAATATCA TCTCTGTACC CGCTGGATGC AACTTGGAGC ATTTTATCCA 
TACTCAAGGA ATCACAACAT TGCAAATACT AGAAGACAAG ATCCCGCTTC CTGGAATGAA 
ACTTTTGCTG AAATGTCAAG GAATATTCTA AATATTAGAT ACACCTTATT GCCCTATTTT 
TACACACAAA TGCATGAAAT TCATGCTAAT GGTGGCACTG TTATCCGACC CCTTTTGCAT 
GAGTTCTTTG ATGAAAAACC AACCTGGGAT ATATTCAAGC AGTTCTTATG GGGTCCAGCA 
TTTATGGTTA CCCCAGTACT GGAACCTTAT GTTCAAACTG TAAATGCCTA CGTCCCCAAT 
GCTCGGTGGT TTGACTACCA TACAGGCAAA GATATTGGCG TCAGAGGACA ATTTCAAACA 
TTTAATGCTT CTTATGACAC AATAAACCTA CATGTCCGTG GTGGTCACAT CCTACCATGT 
CAAGAGCCAG CTCAAAACAC ATTTTACAGT CGACAAAAAC ACATGAAGCT CATTGTTGCT 
GCAGATGATA ATCAGATGGC ACAGGGTTCT CTGTTTTGGG ATGATGGAGA GAGTATAGAC 
ACCTATGAAA GAGACCTATA TTTATCTGTA CAATTTAATT TAAACCAGAC CACCTTAACA 
AGCACTATAT TGAAGAGAGG TTACATAAAT AAAAGTGAAA CGAGGCTTGG ATCCCTTCAT 
GTATGGGGGA AAGGAACTAC TCCTGTCAAT GCAGTTACTC TAACGTATAA CGGAAATAAA 
AATTCGCTTC CTTTTAATGA AGACACTACC AACATGATAT TACGTATTGA TCTGACCACA 
CACAATGTTA CTCTAGAAGA ACCAATAGAA ATCAACTGGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Congenital Sucrase-isomaltase Deficiency: A Novel Compound Heterozygous Mutation Causing Aberrant Protein Localization.
Journal of pediatric gastroenterology and nutrition64(5)770-776(2017 May)
Haberman Y,Di Segni A,Loberman-Nachum N,Barel O,Kunik V,Eyal E,Kol N,Hout-Siloni G,Kochavi B,Avivi C,Schvimer M,Rechavi G,Anikster Y,Barshack I,Weiss B

Molecular pathogenicity of novel sucrase-isomaltase mutations found in congenital sucrase-isomaltase deficiency patients.
Biochimica et biophysica acta1863(3)817-826(2017 Mar)
Gericke B,Amiri M,Scott CR,Naim HY

Congenital sucrase-isomaltase deficiency: identification of a common Inuit founder mutation.
CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne187(2)102-7(2015 Feb)
Marcadier JL,Boland M,Scott CR,Issa K,Wu Z,McIntyre AD,Hegele RA,Geraghty MT,Lines MA

Functional analysis of sucrase-isomaltase mutations from chronic lymphocytic leukemia patients.
Human molecular genetics22(11)2273-82(2013 Jun)
Rodr?guez D,Ramsay AJ,Quesada V,Garabaya C,Campo E,Freije JM,L?pez-Ot?n C

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SI cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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