PLA2G2F cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLA2G2F
Entrez Gene ID	64600
Full Name	phospholipase A2 group IIF
General protein information	Preferred Names phospholipase A2 group IIF Names group IIF secretory phospholipase A2 GIIF sPLA2 phosphatidylcholine 2-acylhydrolase 2F sPLA2-IIF
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.12
Disorder MIM:	616793

mRNA and Protein(s)

mRNA	Protein	Name
NM_022819.3	NP_073730.3	group IIF secretory phospholipase A2 isoform 1
XM_011541956.1	XP_011540258.1	group IIF secretory phospholipase A2 isoform X2
XM_011541955.1	XP_011540257.1	group IIF secretory phospholipase A2 isoform X1
NM_001360869.1	NP_001347798.1	group IIF secretory phospholipase A2 isoform 2 precursor
NM_022819.4	NP_073730.3	group IIF secretory phospholipase A2 isoform 1
NM_001360869.2	NP_001347798.1	group IIF secretory phospholipase A2 isoform 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PLA2G2F	XP_544523.2
Pan troglodytes (chimpanzee)	PLA2G2F	XP_001163707.1
Mus musculus (house mouse)	Pla2g2f	NP_036175.2
Rattus norvegicus (Norway rat)	Pla2g2f	NP_001103057.1
Homo sapiens (human)	PLA2G2F	NP_073730.3
Bos taurus (cattle)	PLA2G2F	NP_001095992.1

Gene Ontology
Bibliography

Related articles in PubMed

sPLA2 and the epidermal barrier.
Ilic D, Bollinger JM, Gelb M, Mauro TM
Biochimica et biophysica acta1841(3)416-21(2014 Mar)

Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment.
Segat L, Brand?o LA, Guimar?es RL, Pontillo A, Athanasakis E, de Oliveira RM, Arraes LC, de Lima Filho JL, Crovella S
Vaccine28(10)2201-6(2010 Mar)

Cellular arachidonate-releasing function and inflammation-associated expression of group IIF secretory phospholipase A2.
Murakami M, Yoshihara K, Shimbara S, Lambeau G, Gelb MH, Singer AG, Sawada M, Inagaki N, Nagai H, Ishihara M, Ishikawa Y, Ishii T, Kudo I
The Journal of biological chemistry277(21)19145-55(2002 May)

Cloning and recombinant expression of human group IIF-secreted phospholipase A(2).
Valentin E, Singer AG, Ghomashchi F, Lazdunski M, Gelb MH, Lambeau G
Biochemical and biophysical research communications279(1)223-8(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

two of these isoforms, sPLA2 IIA and sPLA2 IIF, localize to the upper stratum granulosum and increase in response to experimental barrier perturbation.
Title: sPLA2 and the epidermal barrier.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment.

The following PLA2G2F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLA2G2F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17317	NM_022819.4 Latest version!	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu17317	XM_011541956.1 Latest version!	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu71410	XM_011541955.1 Latest version!	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu106769	NM_001360869.2 Latest version!	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu17317	NM_022819.3	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu106769	NM_001360869.1	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLA2G2F ( NM_022819.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_073730.3 Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17317
Clone ID Related Accession (Same CDS sequence)	NM_022819.3 , XM_011541956.1 , NM_022819.4
Accession Version	NM_022819.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	group IIF secretory phospholipase A2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX647458.1, AF306566.1, AK093645.1, Z98257.1 and BM987713.1. On Apr 20, 2007 this sequence version replaced NM_022819.2. Transcript Variant: This variant (1) encodes a longer protein (isoform 1), which lacks a signal peptide. With the available conservation data, it is not clear which start codon is most likely used. ##Evidence-Data-START## Transcript exon combination :: AK093645.1, DB105026.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGATG GGGCAAAGGC CAACCCCAAA GGGTTCAAAA AGAAGGTGCT GGATAGATGC 
TTCTCTGGGT GGAGGGGCCC ACGCTTCGGG GCCTCCTGTC CTTCAAGAAC CTCCAGGTCT 
AGCCTGGGTA TGAAGAAGTT CTTCACCGTG GCCATCCTTG CTGGCAGCGT TCTGTCCACA 
GCTCACGGCA GCCTGCTCAA CCTGAAGGCC ATGGTGGAGG CCGTCACAGG GAGGAGCGCC 
ATCCTGTCCT TCGTGGGCTA CGGTTGCTAC TGTGGGCTGG GGGGCCGTGG CCAGCCCAAG 
GATGAGGTGG ACTGGTGCTG CCACGCCCAC GACTGCTGCT ACCAGGAACT CTTTGACCAA 
GGCTGTCACC CCTATGTGGA CCACTATGAT CACACCATCG AGAACAACAC TGAGATAGTC 
TGCAGTGACC TCAACAAGAC AGAGTGTGAC AAGCAGACAT GCATGTGTGA CAAGAACATG 
GTTCTGTGCC TCATGAACCA GACGTACCGA GAGGAGTACC GTGGCTTCCT CAATGTCTAC 
TGCCAGGGCC CCACGCCCAA CTGCAGCATC TATGAACCGC CCCCTGAGGA GGTCACCTGC 
AGTCACCAAT CCCCAGCGCC CCCCGCCCCT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073730.3
CDS	99..734
Translation	MADGAKANPKGFKKKVLDRCFSGWRGPRFGASCPSRTSRSSLGMKKFFTVAILAGSVLSTAHGSLLNLKAMVEAVTGRSAILSFVGYGCYCGLGGRGQPKDEVDWCCHAHDCCYQELFDQGCHPYVDHYDHTIENNTEIVCSDLNKTECDKQTCMCDKNMVLCLMNQTYREEYRGFLNVYCQGPTPNCSIYEPPPEEVTCSHQSPAPPAPP

Target ORF information:

RefSeq Version	NM_022819.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022819.3

ORF Insert Sequence:

ATGGCAGATG GGGCAAAGGC CAACCCCAAA GGGTTCAAAA AGAAGGTGCT GGATAGATGC 
TTCTCTGGGT GGAGGGGCCC ACGCTTCGGG GCCTCCTGTC CTTCAAGAAC CTCCAGGTCT 
AGCCTGGGTA TGAAGAAGTT CTTCACCGTG GCCATCCTTG CTGGCAGCGT TCTGTCCACA 
GCTCACGGCA GCCTGCTCAA CCTGAAGGCC ATGGTGGAGG CCGTCACAGG GAGGAGCGCC 
ATCCTGTCCT TCGTGGGCTA CGGTTGCTAC TGTGGGCTGG GGGGCCGTGG CCAGCCCAAG 
GATGAGGTGG ACTGGTGCTG CCACGCCCAC GACTGCTGCT ACCAGGAACT CTTTGACCAA 
GGCTGTCACC CCTATGTGGA CCACTATGAT CACACCATCG AGAACAACAC TGAGATAGTC 
TGCAGTGACC TCAACAAGAC AGAGTGTGAC AAGCAGACAT GCATGTGTGA CAAGAACATG 
GTTCTGTGCC TCATGAACCA GACGTACCGA GAGGAGTACC GTGGCTTCCT CAATGTCTAC 
TGCCAGGGCC CCACGCCCAA CTGCAGCATC TATGAACCGC CCCCTGAGGA GGTCACCTGC 
AGTCACCAAT CCCCAGCGCC CCCCGCCCCT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G2F ( NM_022819.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_073730.3 Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17317
Clone ID Related Accession (Same CDS sequence)	NM_022819.3 , XM_011541956.1 , NM_022819.4
Accession Version	NM_022819.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Homo sapiens(human)
Product	group IIF secretory phospholipase A2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL158172.5, BX647458.1, AF306566.1, AK093645.1, Z98257.1 and BM987713.1. On Jun 2, 2019 this sequence version replaced NM_022819.3. Transcript Variant: This variant (1) encodes a longer protein (isoform 1), which lacks a signal peptide. With the available conservation data, it is not clear which start codon is most likely used. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1344780.1, SRR7346977.1130655.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGATG GGGCAAAGGC CAACCCCAAA GGGTTCAAAA AGAAGGTGCT GGATAGATGC 
TTCTCTGGGT GGAGGGGCCC ACGCTTCGGG GCCTCCTGTC CTTCAAGAAC CTCCAGGTCT 
AGCCTGGGTA TGAAGAAGTT CTTCACCGTG GCCATCCTTG CTGGCAGCGT TCTGTCCACA 
GCTCACGGCA GCCTGCTCAA CCTGAAGGCC ATGGTGGAGG CCGTCACAGG GAGGAGCGCC 
ATCCTGTCCT TCGTGGGCTA CGGTTGCTAC TGTGGGCTGG GGGGCCGTGG CCAGCCCAAG 
GATGAGGTGG ACTGGTGCTG CCACGCCCAC GACTGCTGCT ACCAGGAACT CTTTGACCAA 
GGCTGTCACC CCTATGTGGA CCACTATGAT CACACCATCG AGAACAACAC TGAGATAGTC 
TGCAGTGACC TCAACAAGAC AGAGTGTGAC AAGCAGACAT GCATGTGTGA CAAGAACATG 
GTTCTGTGCC TCATGAACCA GACGTACCGA GAGGAGTACC GTGGCTTCCT CAATGTCTAC 
TGCCAGGGCC CCACGCCCAA CTGCAGCATC TATGAACCGC CCCCTGAGGA GGTCACCTGC 
AGTCACCAAT CCCCAGCGCC CCCCGCCCCT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073730.3
CDS	106..741
Translation	MADGAKANPKGFKKKVLDRCFSGWRGPRFGASCPSRTSRSSLGMKKFFTVAILAGSVLSTAHGSLLNLKAMVEAVTGRSAILSFVGYGCYCGLGGRGQPKDEVDWCCHAHDCCYQELFDQGCHPYVDHYDHTIENNTEIVCSDLNKTECDKQTCMCDKNMVLCLMNQTYREEYRGFLNVYCQGPTPNCSIYEPPPEEVTCSHQSPAPPAPP

Target ORF information:

RefSeq Version	NM_022819.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022819.4

ORF Insert Sequence:

ATGGCAGATG GGGCAAAGGC CAACCCCAAA GGGTTCAAAA AGAAGGTGCT GGATAGATGC 
TTCTCTGGGT GGAGGGGCCC ACGCTTCGGG GCCTCCTGTC CTTCAAGAAC CTCCAGGTCT 
AGCCTGGGTA TGAAGAAGTT CTTCACCGTG GCCATCCTTG CTGGCAGCGT TCTGTCCACA 
GCTCACGGCA GCCTGCTCAA CCTGAAGGCC ATGGTGGAGG CCGTCACAGG GAGGAGCGCC 
ATCCTGTCCT TCGTGGGCTA CGGTTGCTAC TGTGGGCTGG GGGGCCGTGG CCAGCCCAAG 
GATGAGGTGG ACTGGTGCTG CCACGCCCAC GACTGCTGCT ACCAGGAACT CTTTGACCAA 
GGCTGTCACC CCTATGTGGA CCACTATGAT CACACCATCG AGAACAACAC TGAGATAGTC 
TGCAGTGACC TCAACAAGAC AGAGTGTGAC AAGCAGACAT GCATGTGTGA CAAGAACATG 
GTTCTGTGCC TCATGAACCA GACGTACCGA GAGGAGTACC GTGGCTTCCT CAATGTCTAC 
TGCCAGGGCC CCACGCCCAA CTGCAGCATC TATGAACCGC CCCCTGAGGA GGTCACCTGC 
AGTCACCAAT CCCCAGCGCC CCCCGCCCCT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G2F ( XM_011541956.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540258.1 Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu17317
Clone ID Related Accession (Same CDS sequence)	NM_022819.3 , XM_011541956.1 , NM_022819.4
Accession Version	XM_011541956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	group IIF secretory phospholipase A2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG GGGCAAAGGC CAACCCCAAA GGGTTCAAAA AGAAGGTGCT GGATAGATGC 
TTCTCTGGGT GGAGGGGCCC ACGCTTCGGG GCCTCCTGTC CTTCAAGAAC CTCCAGGTCT 
AGCCTGGGTA TGAAGAAGTT CTTCACCGTG GCCATCCTTG CTGGCAGCGT TCTGTCCACA 
GCTCACGGCA GCCTGCTCAA CCTGAAGGCC ATGGTGGAGG CCGTCACAGG GAGGAGCGCC 
ATCCTGTCCT TCGTGGGCTA CGGTTGCTAC TGTGGGCTGG GGGGCCGTGG CCAGCCCAAG 
GATGAGGTGG ACTGGTGCTG CCACGCCCAC GACTGCTGCT ACCAGGAACT CTTTGACCAA 
GGCTGTCACC CCTATGTGGA CCACTATGAT CACACCATCG AGAACAACAC TGAGATAGTC 
TGCAGTGACC TCAACAAGAC AGAGTGTGAC AAGCAGACAT GCATGTGTGA CAAGAACATG 
GTTCTGTGCC TCATGAACCA GACGTACCGA GAGGAGTACC GTGGCTTCCT CAATGTCTAC 
TGCCAGGGCC CCACGCCCAA CTGCAGCATC TATGAACCGC CCCCTGAGGA GGTCACCTGC 
AGTCACCAAT CCCCAGCGCC CCCCGCCCCT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540258.1
CDS	103..738
Translation	MADGAKANPKGFKKKVLDRCFSGWRGPRFGASCPSRTSRSSLGMKKFFTVAILAGSVLSTAHGSLLNLKAMVEAVTGRSAILSFVGYGCYCGLGGRGQPKDEVDWCCHAHDCCYQELFDQGCHPYVDHYDHTIENNTEIVCSDLNKTECDKQTCMCDKNMVLCLMNQTYREEYRGFLNVYCQGPTPNCSIYEPPPEEVTCSHQSPAPPAPP

Target ORF information:

RefSeq Version	XM_011541956.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541956.1

ORF Insert Sequence:

ATGGCAGATG GGGCAAAGGC CAACCCCAAA GGGTTCAAAA AGAAGGTGCT GGATAGATGC 
TTCTCTGGGT GGAGGGGCCC ACGCTTCGGG GCCTCCTGTC CTTCAAGAAC CTCCAGGTCT 
AGCCTGGGTA TGAAGAAGTT CTTCACCGTG GCCATCCTTG CTGGCAGCGT TCTGTCCACA 
GCTCACGGCA GCCTGCTCAA CCTGAAGGCC ATGGTGGAGG CCGTCACAGG GAGGAGCGCC 
ATCCTGTCCT TCGTGGGCTA CGGTTGCTAC TGTGGGCTGG GGGGCCGTGG CCAGCCCAAG 
GATGAGGTGG ACTGGTGCTG CCACGCCCAC GACTGCTGCT ACCAGGAACT CTTTGACCAA 
GGCTGTCACC CCTATGTGGA CCACTATGAT CACACCATCG AGAACAACAC TGAGATAGTC 
TGCAGTGACC TCAACAAGAC AGAGTGTGAC AAGCAGACAT GCATGTGTGA CAAGAACATG 
GTTCTGTGCC TCATGAACCA GACGTACCGA GAGGAGTACC GTGGCTTCCT CAATGTCTAC 
TGCCAGGGCC CCACGCCCAA CTGCAGCATC TATGAACCGC CCCCTGAGGA GGTCACCTGC 
AGTCACCAAT CCCCAGCGCC CCCCGCCCCT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G2F ( XM_011541955.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540257.1 Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu71410
Clone ID Related Accession (Same CDS sequence)	XM_011541955.1
Accession Version	XM_011541955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	group IIF secretory phospholipase A2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG GGGCAAAGGC CAACCCCAAA GGGTTCAAAA AGAAGGTGCT GGATAGATGC 
TTCTCTGGGT GGAGGGGCCC ACGCTTCGGG GCCTCCTGTC CTTCAAGAAC CTCCAGGTCT 
AGCCTGGGTA TGAAGAAGTT CTTCACCGTG GCCATCCTTG CTGGCAGCGT TCTGTCCACA 
GCTCACGGCA GCCTGCTCAA CCTGAAGGCC ATGGTGGAGG CCGTCACAGG GAGGAGCGCC 
ATCCTGTCCT TCGTGGGCTA CGGTTGCTAC TGTGGGCTGG GGGGCCGTGG CCAGCCCAAG 
GATGAGGTGG ACTGGTGCTG CCACGCCCAC GACTGCTGCT ACCAGGAACT CTTTGACCAA 
GGCTGTCACC CCTATGTGGA CCACTATGAT CACACCATCG AGAACAACAC TGAGATAGTC 
TGCAGTGCAC CTTACCTTCC TCCTTACCTG CCCGAGCCTC AACTCAGGGT CAGCAGCCTC 
CACGGAGCCA GCCCCACCTG CCCGAAAACC ACTGAAGCCA CCCTCCGCCC GGACTCAACA 
GCCGCCAGCA AGCTCGCCTG GCAGTGCCCC TGGAGCACCA TTGTCGCTGG GGAGGGAGAG 
CCGGGAAGGG TGGAGAAGGA GGCAGAAGGG ACATGTATGA AAAAAGGACA GGAAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540257.1
CDS	92..751
Translation	MADGAKANPKGFKKKVLDRCFSGWRGPRFGASCPSRTSRSSLGMKKFFTVAILAGSVLSTAHGSLLNLKAMVEAVTGRSAILSFVGYGCYCGLGGRGQPKDEVDWCCHAHDCCYQELFDQGCHPYVDHYDHTIENNTEIVCSAPYLPPYLPEPQLRVSSLHGASPTCPKTTEATLRPDSTAASKLAWQCPWSTIVAGEGEPGRVEKEAEGTCMKKGQET

Target ORF information:

RefSeq Version	XM_011541955.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541955.1

ORF Insert Sequence:

ATGGCAGATG GGGCAAAGGC CAACCCCAAA GGGTTCAAAA AGAAGGTGCT GGATAGATGC 
TTCTCTGGGT GGAGGGGCCC ACGCTTCGGG GCCTCCTGTC CTTCAAGAAC CTCCAGGTCT 
AGCCTGGGTA TGAAGAAGTT CTTCACCGTG GCCATCCTTG CTGGCAGCGT TCTGTCCACA 
GCTCACGGCA GCCTGCTCAA CCTGAAGGCC ATGGTGGAGG CCGTCACAGG GAGGAGCGCC 
ATCCTGTCCT TCGTGGGCTA CGGTTGCTAC TGTGGGCTGG GGGGCCGTGG CCAGCCCAAG 
GATGAGGTGG ACTGGTGCTG CCACGCCCAC GACTGCTGCT ACCAGGAACT CTTTGACCAA 
GGCTGTCACC CCTATGTGGA CCACTATGAT CACACCATCG AGAACAACAC TGAGATAGTC 
TGCAGTGCAC CTTACCTTCC TCCTTACCTG CCCGAGCCTC AACTCAGGGT CAGCAGCCTC 
CACGGAGCCA GCCCCACCTG CCCGAAAACC ACTGAAGCCA CCCTCCGCCC GGACTCAACA 
GCCGCCAGCA AGCTCGCCTG GCAGTGCCCC TGGAGCACCA TTGTCGCTGG GGAGGGAGAG 
CCGGGAAGGG TGGAGAAGGA GGCAGAAGGG ACATGTATGA AAAAAGGACA GGAAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G2F ( NM_001360869.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001347798.1 Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1a, mRNA.

Price & Availability↑

CloneID	OHu106769
Clone ID Related Accession (Same CDS sequence)	NM_001360869.1 , NM_001360869.2
Accession Version	NM_001360869.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	group IIF secretory phospholipase A2 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX647458.1, AK093645.1, AF306566.1, Z98257.1 and BM987713.1. Transcript Variant: This variant (1a) reflects the use of an in-frame downstream start codon for translation, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus with a predicted signal peptide, compared to isoform 1. With the available conservation data, it is not clear which start codon is most likely used. ##Evidence-Data-START## Transcript exon combination :: AK093645.1, DB105026.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGAAGT TCTTCACCGT GGCCATCCTT GCTGGCAGCG TTCTGTCCAC AGCTCACGGC 
AGCCTGCTCA ACCTGAAGGC CATGGTGGAG GCCGTCACAG GGAGGAGCGC CATCCTGTCC 
TTCGTGGGCT ACGGTTGCTA CTGTGGGCTG GGGGGCCGTG GCCAGCCCAA GGATGAGGTG 
GACTGGTGCT GCCACGCCCA CGACTGCTGC TACCAGGAAC TCTTTGACCA AGGCTGTCAC 
CCCTATGTGG ACCACTATGA TCACACCATC GAGAACAACA CTGAGATAGT CTGCAGTGAC 
CTCAACAAGA CAGAGTGTGA CAAGCAGACA TGCATGTGTG ACAAGAACAT GGTTCTGTGC 
CTCATGAACC AGACGTACCG AGAGGAGTAC CGTGGCTTCC TCAATGTCTA CTGCCAGGGC 
CCCACGCCCA ACTGCAGCAT CTATGAACCG CCCCCTGAGG AGGTCACCTG CAGTCACCAA 
TCCCCAGCGC CCCCCGCCCC TCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347798.1
CDS	228..734
Translation	MKKFFTVAILAGSVLSTAHGSLLNLKAMVEAVTGRSAILSFVGYGCYCGLGGRGQPKDEVDWCCHAHDCCYQELFDQGCHPYVDHYDHTIENNTEIVCSDLNKTECDKQTCMCDKNMVLCLMNQTYREEYRGFLNVYCQGPTPNCSIYEPPPEEVTCSHQSPAPPAPP

Target ORF information:

RefSeq Version	NM_001360869.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360869.1

ORF Insert Sequence:

ATGAAGAAGT TCTTCACCGT GGCCATCCTT GCTGGCAGCG TTCTGTCCAC AGCTCACGGC 
AGCCTGCTCA ACCTGAAGGC CATGGTGGAG GCCGTCACAG GGAGGAGCGC CATCCTGTCC 
TTCGTGGGCT ACGGTTGCTA CTGTGGGCTG GGGGGCCGTG GCCAGCCCAA GGATGAGGTG 
GACTGGTGCT GCCACGCCCA CGACTGCTGC TACCAGGAAC TCTTTGACCA AGGCTGTCAC 
CCCTATGTGG ACCACTATGA TCACACCATC GAGAACAACA CTGAGATAGT CTGCAGTGAC 
CTCAACAAGA CAGAGTGTGA CAAGCAGACA TGCATGTGTG ACAAGAACAT GGTTCTGTGC 
CTCATGAACC AGACGTACCG AGAGGAGTAC CGTGGCTTCC TCAATGTCTA CTGCCAGGGC 
CCCACGCCCA ACTGCAGCAT CTATGAACCG CCCCCTGAGG AGGTCACCTG CAGTCACCAA 
TCCCCAGCGC CCCCCGCCCC TCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G2F ( NM_001360869.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001347798.1 Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1a, mRNA.

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CloneID	OHu106769
Clone ID Related Accession (Same CDS sequence)	NM_001360869.1 , NM_001360869.2
Accession Version	NM_001360869.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	group IIF secretory phospholipase A2 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL158172.5, BX647458.1, AK093645.1, AF306566.1, Z98257.1 and BM987713.1. On Jun 2, 2019 this sequence version replaced NM_001360869.1. Transcript Variant: This variant (1a) reflects the use of an in-frame downstream start codon for translation, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus with a predicted signal peptide, compared to isoform 1. With the available conservation data, it is not clear which start codon is most likely used. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1344780.1, SRR7346977.1130655.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGAAGT TCTTCACCGT GGCCATCCTT GCTGGCAGCG TTCTGTCCAC AGCTCACGGC 
AGCCTGCTCA ACCTGAAGGC CATGGTGGAG GCCGTCACAG GGAGGAGCGC CATCCTGTCC 
TTCGTGGGCT ACGGTTGCTA CTGTGGGCTG GGGGGCCGTG GCCAGCCCAA GGATGAGGTG 
GACTGGTGCT GCCACGCCCA CGACTGCTGC TACCAGGAAC TCTTTGACCA AGGCTGTCAC 
CCCTATGTGG ACCACTATGA TCACACCATC GAGAACAACA CTGAGATAGT CTGCAGTGAC 
CTCAACAAGA CAGAGTGTGA CAAGCAGACA TGCATGTGTG ACAAGAACAT GGTTCTGTGC 
CTCATGAACC AGACGTACCG AGAGGAGTAC CGTGGCTTCC TCAATGTCTA CTGCCAGGGC 
CCCACGCCCA ACTGCAGCAT CTATGAACCG CCCCCTGAGG AGGTCACCTG CAGTCACCAA 
TCCCCAGCGC CCCCCGCCCC TCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347798.1
CDS	235..741
Translation	MKKFFTVAILAGSVLSTAHGSLLNLKAMVEAVTGRSAILSFVGYGCYCGLGGRGQPKDEVDWCCHAHDCCYQELFDQGCHPYVDHYDHTIENNTEIVCSDLNKTECDKQTCMCDKNMVLCLMNQTYREEYRGFLNVYCQGPTPNCSIYEPPPEEVTCSHQSPAPPAPP

Target ORF information:

RefSeq Version	NM_001360869.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A2 group IIF (PLA2G2F), transcript variant 1a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360869.2

ORF Insert Sequence:

ATGAAGAAGT TCTTCACCGT GGCCATCCTT GCTGGCAGCG TTCTGTCCAC AGCTCACGGC 
AGCCTGCTCA ACCTGAAGGC CATGGTGGAG GCCGTCACAG GGAGGAGCGC CATCCTGTCC 
TTCGTGGGCT ACGGTTGCTA CTGTGGGCTG GGGGGCCGTG GCCAGCCCAA GGATGAGGTG 
GACTGGTGCT GCCACGCCCA CGACTGCTGC TACCAGGAAC TCTTTGACCA AGGCTGTCAC 
CCCTATGTGG ACCACTATGA TCACACCATC GAGAACAACA CTGAGATAGT CTGCAGTGAC 
CTCAACAAGA CAGAGTGTGA CAAGCAGACA TGCATGTGTG ACAAGAACAT GGTTCTGTGC 
CTCATGAACC AGACGTACCG AGAGGAGTAC CGTGGCTTCC TCAATGTCTA CTGCCAGGGC 
CCCACGCCCA ACTGCAGCAT CTATGAACCG CCCCCTGAGG AGGTCACCTG CAGTCACCAA 
TCCCCAGCGC CCCCCGCCCC TCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

sPLA2 and the epidermal barrier.
Biochimica et biophysica acta1841(3)416-21(2014 Mar)
Ilic D,Bollinger JM,Gelb M,Mauro TM

Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment.
Vaccine28(10)2201-6(2010 Mar)
Segat L,Brand?o LA,Guimar?es RL,Pontillo A,Athanasakis E,de Oliveira RM,Arraes LC,de Lima Filho JL,Crovella S

Cellular arachidonate-releasing function and inflammation-associated expression of group IIF secretory phospholipase A2.
The Journal of biological chemistry277(21)19145-55(2002 May)
Murakami M,Yoshihara K,Shimbara S,Lambeau G,Gelb MH,Singer AG,Sawada M,Inagaki N,Nagai H,Ishihara M,Ishikawa Y,Ishii T,Kudo I

Cloning and recombinant expression of human group IIF-secreted phospholipase A(2).
Biochemical and biophysical research communications279(1)223-8(2000 Dec)
Valentin E,Singer AG,Ghomashchi F,Lazdunski M,Gelb MH,Lambeau G

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PLA2G2F cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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