SCG5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCG5
Entrez Gene ID	6447
Full Name	secretogranin V
Synonyms	7B2,P7B2,SGNE1,SgV
General protein information	Preferred Names secretogranin V Names neuroendocrine protein 7B2 pituitary polypeptide prohormone convertase chaperone secretogranin-5 secretory granule endocrine protein I secretory granule, neuroendocrine protein 1 (7B2 protein)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q13.3
Summary	This gene encodes a secreted chaperone protein that prevents the aggregation of other secreted proteins, including proteins that are associated with neurodegenerative and metabolic disease. The encoded protein may be best known for its role in the trafficking and activation of prohormone convertase PC2 (encoded by Gene ID: 5126). Phosphorylation of the encoded protein has been shown to have an inhibitory effect on its chaperone function. [provided by RefSeq, Jul 2016].
Disorder MIM:	173120

mRNA and Protein(s)

mRNA	Protein	Name
NM_003020.4	NP_003011.1	neuroendocrine protein 7B2 isoform 2 precursor
NM_001144757.2	NP_001138229.1	neuroendocrine protein 7B2 isoform 1 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Scg5	NP_033188.3
Xenopus tropicalis (tropical clawed frog)	scg5	NP_001072162.2
Homo sapiens (human)	SCG5	NP_001138229.1
Pan troglodytes (chimpanzee)	SCG5	NP_001092019.1
	SCG5	XP_001083853.1
Danio rerio (zebrafish)	LOC100330746	XP_002665782.1
Gallus gallus (chicken)	SCG5	XP_004941787.1
Danio rerio (zebrafish)	scg5	NP_957020.1
Rattus norvegicus (Norway rat)	Scg5	NP_037307.1

Gene Ontology
Bibliography

Related articles in PubMed

Clinicopathological features of a kindred with SCG5-GREM1-associated hereditary mixed polyposis syndrome.
Plesec T, Brown K, Allen C, A Burke C, Church J, Kalady M, LaGuardia L, O'Malley M, Heald B
Human pathology6075-81(2017 02)

Phosphorylation and Alternative Splicing of 7B2 Reduce Prohormone Convertase 2 Activation.
Ramos-Molina B, Lindberg I
Molecular endocrinology (Baltimore, Md.)29(5)756-64(2015 May)

Blockade of islet amyloid polypeptide fibrillation and cytotoxicity by the secretory chaperones 7B2 and proSAAS.
Peinado JR, Sami F, Rajpurohit N, Lindberg I
FEBS letters587(21)3406-11(2013 Nov)

The neuroendocrine protein 7B2 suppresses the aggregation of neurodegenerative disease-related proteins.
Helwig M, Hoshino A, Berridge C, Lee SN, Lorenzen N, Otzen DE, Eriksen JL, Lindberg I
The Journal of biological chemistry288(2)1114-24(2013 Jan)

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA, Butte NF
PloS one7(12)e51954(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Describe a hereditary mixed polyposis syndrome in which is characterized by SCG5-GREM1 duplication.
Title: Clinicopathological features of a kindred with SCG5-GREM1-associated hereditary mixed polyposis syndrome.

FAM20C plays a role in 7B2-mediated proPC2 activation by phosphorylating residue Thr111; and that 7B2 function is regulated by alternative splicing.
Title: Phosphorylation and Alternative Splicing of 7B2 Reduce Prohormone Convertase 2 Activation.

7B2 chaperones blocked the cytotoxic effects of exogenous hIAPP
Title: Blockade of islet amyloid polypeptide fibrillation and cytotoxicity by the secretory chaperones 7B2 and proSAAS.

data provide insight into novel functions of 7B2 and establish this neural protein as an anti-aggregation chaperone associated with neurodegenerative disease
Title: The neuroendocrine protein 7B2 suppresses the aggregation of neurodegenerative disease-related proteins.

conclude that 7B2 is a natively disordered protein whose function as an antiaggregant chaperone is likely facilitated by its lack of appreciable secondary structure and tendency to form oligomers
Title: The neuroendocrine protein 7B2 is intrinsically disordered.

The following SCG5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCG5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu02240	NM_001144757.2 Latest version!	Homo sapiens secretogranin V (SCG5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu04042	NM_003020.4 Latest version!	Homo sapiens secretogranin V (SCG5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCG5 ( NM_001144757.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138229.1 Homo sapiens secretogranin V (SCG5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02240
Clone ID Related Accession (Same CDS sequence)	NM_001144757.2
Accession Version	NM_001144757.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	neuroendocrine protein 7B2 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP308267.1, BC005349.1, Y00757.1 and R44275.1. This sequence is a reference standard in the RefSeqGene project. On Jul 20, 2016 this sequence version replaced NM_001144757.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.287654.1, BC005349.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCTCCA GGATGGTCTC TACCATGCTA TCTGGCCTAC TGTTTTGGCT GGCATCTGGA 
TGGACTCCAG CATTTGCTTA CAGCCCCCGG ACCCCTGACC GGGTCTCAGA AGCAGATATC 
CAGAGGCTGC TTCATGGTGT TATGGAGCAA TTGGGCATTG CCAGGCCCCG AGTGGAATAT 
CCAGCTCACC AGGCCATGAA TCTTGTGGGC CCCCAGAGCA TTGAAGGTGG AGCTCATGAA 
GGACTTCAGC ATTTGGGTCC TTTTGGCAAC ATCCCCAACA TCGTGGCAGA GTTGACTGGA 
GACAACATTC CTAAGGACTT TAGTGAGGAT CAGGGGTACC CAGACCCTCC AAATCCCTGT 
CCTGTTGGAA AAACAGCAGA TGATGGATGT CTAGAAAACA CCCCTGACAC TGCAGAGTTC 
AGTCGAGAGT TCCAGTTGCA CCAGCATCTC TTTGATCCGG AACATGACTA TCCAGGCTTG 
GGCAAGTGGA ACAAGAAACT CCTTTACGAG AAGATGAAGG GAGGAGAGAG ACGAAAGCGG 
AGGAGTGTCA ATCCATATCT ACAAGGACAG AGACTGGATA ATGTTGTTGC AAAGAAGTCT 
GTCCCCCATT TTTCAGATGA GGATAAGGAT CCAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138229.1
CDS	174..812
Translation	MVSRMVSTMLSGLLFWLASGWTPAFAYSPRTPDRVSEADIQRLLHGVMEQLGIARPRVEYPAHQAMNLVGPQSIEGGAHEGLQHLGPFGNIPNIVAELTGDNIPKDFSEDQGYPDPPNPCPVGKTADDGCLENTPDTAEFSREFQLHQHLFDPEHDYPGLGKWNKKLLYEKMKGGERRKRRSVNPYLQGQRLDNVVAKKSVPHFSDEDKDPE

Target ORF information:

RefSeq Version	NM_001144757.2
Organism	Homo sapiens(human)
Definition	Homo sapiens secretogranin V (SCG5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144757.2

ORF Insert Sequence:

ATGGTCTCCA GGATGGTCTC TACCATGCTA TCTGGCCTAC TGTTTTGGCT GGCATCTGGA 
TGGACTCCAG CATTTGCTTA CAGCCCCCGG ACCCCTGACC GGGTCTCAGA AGCAGATATC 
CAGAGGCTGC TTCATGGTGT TATGGAGCAA TTGGGCATTG CCAGGCCCCG AGTGGAATAT 
CCAGCTCACC AGGCCATGAA TCTTGTGGGC CCCCAGAGCA TTGAAGGTGG AGCTCATGAA 
GGACTTCAGC ATTTGGGTCC TTTTGGCAAC ATCCCCAACA TCGTGGCAGA GTTGACTGGA 
GACAACATTC CTAAGGACTT TAGTGAGGAT CAGGGGTACC CAGACCCTCC AAATCCCTGT 
CCTGTTGGAA AAACAGCAGA TGATGGATGT CTAGAAAACA CCCCTGACAC TGCAGAGTTC 
AGTCGAGAGT TCCAGTTGCA CCAGCATCTC TTTGATCCGG AACATGACTA TCCAGGCTTG 
GGCAAGTGGA ACAAGAAACT CCTTTACGAG AAGATGAAGG GAGGAGAGAG ACGAAAGCGG 
AGGAGTGTCA ATCCATATCT ACAAGGACAG AGACTGGATA ATGTTGTTGC AAAGAAGTCT 
GTCCCCCATT TTTCAGATGA GGATAAGGAT CCAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCG5 ( NM_003020.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003011.1 Homo sapiens secretogranin V (SCG5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04042
Clone ID Related Accession (Same CDS sequence)	NM_003020.4
Accession Version	NM_003020.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	neuroendocrine protein 7B2 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP308267.1, Y00757.1 and R44275.1. On Jul 20, 2016 this sequence version replaced NM_003020.3. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC093053.1, Y00757.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTCTCCA GGATGGTCTC TACCATGCTA TCTGGCCTAC TGTTTTGGCT GGCATCTGGA 
TGGACTCCAG CATTTGCTTA CAGCCCCCGG ACCCCTGACC GGGTCTCAGA AGCAGATATC 
CAGAGGCTGC TTCATGGTGT TATGGAGCAA TTGGGCATTG CCAGGCCCCG AGTGGAATAT 
CCAGCTCACC AGGCCATGAA TCTTGTGGGC CCCCAGAGCA TTGAAGGTGG AGCTCATGAA 
GGACTTCAGC ATTTGGGTCC TTTTGGCAAC ATCCCCAACA TCGTGGCAGA GTTGACTGGA 
GACAACATTC CTAAGGACTT TAGTGAGGAT CAGGGGTACC CAGACCCTCC AAATCCCTGT 
CCTGTTGGAA AAACAGATGA TGGATGTCTA GAAAACACCC CTGACACTGC AGAGTTCAGT 
CGAGAGTTCC AGTTGCACCA GCATCTCTTT GATCCGGAAC ATGACTATCC AGGCTTGGGC 
AAGTGGAACA AGAAACTCCT TTACGAGAAG ATGAAGGGAG GAGAGAGACG AAAGCGGAGG 
AGTGTCAATC CATATCTACA AGGACAGAGA CTGGATAATG TTGTTGCAAA GAAGTCTGTC 
CCCCATTTTT CAGATGAGGA TAAGGATCCA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003011.1
CDS	174..809
Translation	MVSRMVSTMLSGLLFWLASGWTPAFAYSPRTPDRVSEADIQRLLHGVMEQLGIARPRVEYPAHQAMNLVGPQSIEGGAHEGLQHLGPFGNIPNIVAELTGDNIPKDFSEDQGYPDPPNPCPVGKTDDGCLENTPDTAEFSREFQLHQHLFDPEHDYPGLGKWNKKLLYEKMKGGERRKRRSVNPYLQGQRLDNVVAKKSVPHFSDEDKDPE

Target ORF information:

RefSeq Version	NM_003020.4
Organism	Homo sapiens(human)
Definition	Homo sapiens secretogranin V (SCG5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003020.4

ORF Insert Sequence:

ATGGTCTCCA GGATGGTCTC TACCATGCTA TCTGGCCTAC TGTTTTGGCT GGCATCTGGA 
TGGACTCCAG CATTTGCTTA CAGCCCCCGG ACCCCTGACC GGGTCTCAGA AGCAGATATC 
CAGAGGCTGC TTCATGGTGT TATGGAGCAA TTGGGCATTG CCAGGCCCCG AGTGGAATAT 
CCAGCTCACC AGGCCATGAA TCTTGTGGGC CCCCAGAGCA TTGAAGGTGG AGCTCATGAA 
GGACTTCAGC ATTTGGGTCC TTTTGGCAAC ATCCCCAACA TCGTGGCAGA GTTGACTGGA 
GACAACATTC CTAAGGACTT TAGTGAGGAT CAGGGGTACC CAGACCCTCC AAATCCCTGT 
CCTGTTGGAA AAACAGATGA TGGATGTCTA GAAAACACCC CTGACACTGC AGAGTTCAGT 
CGAGAGTTCC AGTTGCACCA GCATCTCTTT GATCCGGAAC ATGACTATCC AGGCTTGGGC 
AAGTGGAACA AGAAACTCCT TTACGAGAAG ATGAAGGGAG GAGAGAGACG AAAGCGGAGG 
AGTGTCAATC CATATCTACA AGGACAGAGA CTGGATAATG TTGTTGCAAA GAAGTCTGTC 
CCCCATTTTT CAGATGAGGA TAAGGATCCA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clinicopathological features of a kindred with SCG5-GREM1-associated hereditary mixed polyposis syndrome.
Human pathology6075-81(2017 02)
Plesec T,Brown K,Allen C,A Burke C,Church J,Kalady M,LaGuardia L,O'Malley M,Heald B

Phosphorylation and Alternative Splicing of 7B2 Reduce Prohormone Convertase 2 Activation.
Molecular endocrinology (Baltimore, Md.)29(5)756-64(2015 May)
Ramos-Molina B,Lindberg I

Blockade of islet amyloid polypeptide fibrillation and cytotoxicity by the secretory chaperones 7B2 and proSAAS.
FEBS letters587(21)3406-11(2013 Nov)
Peinado JR,Sami F,Rajpurohit N,Lindberg I

The neuroendocrine protein 7B2 suppresses the aggregation of neurodegenerative disease-related proteins.
The Journal of biological chemistry288(2)1114-24(2013 Jan)
Helwig M,Hoshino A,Berridge C,Lee SN,Lorenzen N,Otzen DE,Eriksen JL,Lindberg I

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
PloS one7(12)e51954(2012)
Comuzzie AG,Cole SA,Laston SL,Voruganti VS,Haack K,Gibbs RA,Butte NF

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SCG5 cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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