MMP25 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MMP25
Entrez Gene ID	64386
Full Name	matrix metallopeptidase 25
Synonyms	MMP-25,MMP20,MMP20A,MMPL1,MT-MMP 6,MT-MMP6,MT6-MMP,MT6MMP,MTMMP6
General protein information	Preferred Names matrix metallopeptidase 25 Names matrix metalloproteinase-25 leukolysin matrix metallopeptidase-like 1 matrix metalloproteinase 20 matrix metalloproteinase-like 1 membrane-type 6 matrix metalloproteinase membrane-type matrix metalloproteinase 6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25. [provided by RefSeq, Jul 2008].
Disorder MIM:	608482

mRNA and Protein(s)

mRNA	Protein	Name
XM_024450390.1	XP_024306158.1	matrix metalloproteinase-25 isoform X1
NM_022468.4	NP_071913.1	matrix metalloproteinase-25 preproprotein
XM_024450391.1	XP_024306159.1	matrix metalloproteinase-25 isoform X2
XM_011522605.2	XP_011520907.1	matrix metalloproteinase-25 isoform X5
XM_011522602.2	XP_011520904.1	matrix metalloproteinase-25 isoform X1
XM_011522604.2	XP_011520906.1	matrix metalloproteinase-25 isoform X3
XM_017023561.1	XP_016879050.1	matrix metalloproteinase-25 isoform X4
NM_022468.5	NP_071913.1	matrix metalloproteinase-25 preproprotein

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100333601	XP_002663942.1
Xenopus tropicalis (tropical clawed frog)	mmp25	XP_002943093.2
Pan troglodytes (chimpanzee)	MMP25	XP_001165615.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003929	XP_554330.4
Homo sapiens (human)	MMP25	NP_071913.1
Canis lupus familiaris (dog)	MMP25	XP_547166.3
Bos taurus (cattle)	MMP25	XP_005224698.1
Mus musculus (house mouse)	Mmp25	NP_001028511.1
Danio rerio (zebrafish)	mmp25a	XP_001341682.4

Gene Ontology
Bibliography

Related articles in PubMed

Expression of the matrix metalloproteases 2, 14, 24, and 25 and tissue inhibitor 3 as potential molecular markers in advanced human gastric cancer.
de la Pe?a S, Sampieri CL, Ochoa-Lara M, Le?n-C?rdoba K, Remes-Troche JM
Disease markers2014285906(2014)

Membrane-type 6 matrix metalloproteinase regulates the activation-induced downmodulation of CD16 in human primary NK cells.
Peruzzi G, Femnou L, Gil-Krzewska A, Borrego F, Weck J, Krzewski K, Coligan JE
Journal of immunology (Baltimore, Md. : 1950)191(4)1883-94(2013 Aug)

Biochemical characterization and N-terminomics analysis of leukolysin, the membrane-type 6 matrix metalloprotease (MMP25): chemokine and vimentin cleavages enhance cell migration and macrophage phagocytic activities.
Starr AE, Bellac CL, Dufour A, Goebeler V, Overall CM
The Journal of biological chemistry287(16)13382-95(2012 Apr)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

Genetic variants in COL2A1, COL11A2, and IRF6 contribute risk to nonsyndromic cleft palate.
Nikopensius T, Jagom?gi T, Krjutskov K, Tammekivi V, Saag M, Prane I, Piekuse L, Akota I, Barkane B, Krumina A, Ambrozaityte L, Matuleviciene A, Kucinskiene ZA, Lace B, Kucinskas V, Metspalu A
Birth defects research. Part A, Clinical and molecular teratology88(9)748-56(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In absolute mRNA levels, significant differences were found in expression of MMP2, MMP24, and MMP25 in gastric carcinoma compared with superficial gastritis.
Title: Expression of the matrix metalloproteases 2, 14, 24, and 25 and tissue inhibitor 3 as potential molecular markers in advanced human gastric cancer.

IL-2-upregulated MT6 cell-surface expression correlates with CD16 downmodulation. MT6, sequestered in intracellular compartments in unstimulated NK cells, translocates to the effector-target cell interface of CD16-mediated immunological synapses.
Title: Membrane-type 6 matrix metalloproteinase regulates the activation-induced downmodulation of CD16 in human primary NK cells.

MT6-MMP regulates neutrophil and monocyte chemotaxis and by generating "eat-me" signals upon vimentin cleavage potentially increases phagocytic removal of neutrophils to resolve inflammation.
Title: Biochemical characterization and N-terminomics analysis of leukolysin, the membrane-type 6 matrix metalloprotease (MMP25): chemokine and vimentin cleavages enhance cell migration and macrophage phagocytic activities.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in COL2A1, COL11A2, and IRF6 contribute risk to nonsyndromic cleft palate.

The following MMP25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMP25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13269	NM_022468.5 Latest version!	Homo sapiens matrix metallopeptidase 25 (MMP25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu71364	XM_024450391.1 Latest version!	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu71364	XM_011522602.2 Latest version!	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu71366	XM_011522604.2 Latest version!	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu71367	XM_011522605.2 Latest version!	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu71363	XM_024450390.1 Latest version!	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu86216	XM_017023561.1 Latest version!	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu13269	NM_022468.4	Homo sapiens matrix metallopeptidase 25 (MMP25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MMP25 ( NM_022468.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071913.1 Homo sapiens matrix metallopeptidase 25 (MMP25), mRNA.

Price & Availability↑

CloneID	OHu13269
Clone ID Related Accession (Same CDS sequence)	NM_022468.4 , NM_022468.5
Accession Version	NM_022468.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	matrix metalloproteinase-25 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC108134.3, AJ272137.1 and CB529891.1. On Aug 19, 2005 this sequence version replaced NM_022468.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ272137.1, AB042328.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTGC GGCTCCGGCT TCTGGCGCTG CTGCTTCTGC TGCTGGCACC GCCCGCGCGC 
GCCCCGAAGC CCTCGGCGCA GGACGTGAGC CTGGGCGTGG ACTGGCTGAC TCGCTATGGT 
TACCTGCCGC CACCCCACCC TGCCCAGGCC CAGCTGCAGA GCCCTGAGAA GTTGCGCGAT 
GCCATCAAAG TCATGCAGAG GTTCGCGGGG CTGCCGGAGA CCGGCCGCAT GGACCCAGGG 
ACAGTGGCCA CCATGCGTAA GCCCCGCTGC TCCCTGCCTG ACGTGCTGGG GGTGGCGGGG 
CTGGTCAGGC GGCGTCGCCG GTACGCTCTG AGCGGCAGCG TGTGGAAGAA GCGAACCCTG 
ACATGGAGGG TACGTTCCTT CCCCCAGAGC TCCCAGCTGA GCCAGGAGAC CGTGCGGGTC 
CTCATGAGCT ATGCCCTGAT GGCCTGGGGC ATGGAGTCAG GCCTCACATT TCATGAGGTG 
GATTCCCCCC AGGGCCAGGA GCCCGACATC CTCATCGACT TTGCCCGCGC CTTCCACCAG 
GACAGCTACC CCTTCGACGG GTTGGGGGGC ACCCTAGCCC ATGCCTTCTT CCCTGGGGAG 
CACCCCATCT CCGGGGACAC TCACTTTGAC GATGAGGAGA CCTGGACTTT TGGGTCAAAA 
GACGGCGAGG GGACCGACCT GTTTGCCGTG GCTGTCCATG AGTTTGGCCA CGCCCTGGGC 
CTGGGCCACT CCTCAGCCCC CAACTCCATT ATGAGGCCCT TCTACCAGGG TCCGGTGGGC 
GACCCTGACA AGTACCGCCT GTCTCAGGAT GACCGCGATG GCCTGCAGCA ACTCTATGGG 
AAGGCGCCCC AAACCCCATA TGACAAGCCC ACAAGGAAAC CCCTGGCTCC TCCGCCCCAG 
CCCCCGGCCT CGCCCACACA CAGCCCATCC TTCCCCATCC CTGATCGATG TGAGGGCAAT 
TTTGACGCCA TCGCCAACAT CCGAGGGGAA ACTTTCTTCT TCAAAGGCCC CTGGTTCTGG 
CGCCTCCAGC CCTCCGGACA GCTGGTGTCC CCGCGACCCG CACGGCTGCA CCGCTTCTGG 
GAGGGGCTGC CCGCCCAGGT GAGGGTGGTG CAGGCCGCCT ATGCTCGGCA CCGAGACGGC 
CGAATCCTCC TCTTTAGCGG GCCCCAGTTC TGGGTGTTCC AGGACCGGCA GCTGGAGGGC 
GGGGCGCGGC CGCTCACGGA GCTGGGGCTG CCCCCGGGAG AGGAGGTGGA CGCCGTGTTC 
TCGTGGCCAC AGAACGGGAA GACCTACCTG GTCCGCGGCC GGCAGTACTG GCGCTACGAC 
GAGGCGGCGG CGCGCCCGGA CCCCGGCTAC CCTCGCGACC TGAGCCTCTG GGAAGGCGCG 
CCCCCCTCCC CTGACGATGT CACCGTCAGC AACGCAGGTG ACACCTACTT CTTCAAGGGC 
GCCCACTACT GGCGCTTCCC CAAGAACAGC ATCAAGACCG AGCCGGACGC CCCCCAGCCC 
ATGGGGCCCA ACTGGCTGGA CTGCCCCGCC CCGAGCTCTG GTCCCCGCGC CCCCAGGCCC 
CCCAAAGCGA CCCCCGTGTC CGAAACCTGC GATTGTCAGT GCGAGCTCAA CCAGGCCGCA 
GGACGTTGGC CTGCTCCCAT CCCGCTGCTC CTCTTGCCCC TGCTGGTGGG GGGTGTAGCC 
TCCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071913.1
CDS	238..1926
Translation	MRLRLRLLALLLLLLAPPARAPKPSAQDVSLGVDWLTRYGYLPPPHPAQAQLQSPEKLRDAIKVMQRFAGLPETGRMDPGTVATMRKPRCSLPDVLGVAGLVRRRRRYALSGSVWKKRTLTWRVRSFPQSSQLSQETVRVLMSYALMAWGMESGLTFHEVDSPQGQEPDILIDFARAFHQDSYPFDGLGGTLAHAFFPGEHPISGDTHFDDEETWTFGSKDGEGTDLFAVAVHEFGHALGLGHSSAPNSIMRPFYQGPVGDPDKYRLSQDDRDGLQQLYGKAPQTPYDKPTRKPLAPPPQPPASPTHSPSFPIPDRCEGNFDAIANIRGETFFFKGPWFWRLQPSGQLVSPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGGARPLTELGLPPGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLSLWEGAPPSPDDVTVSNAGDTYFFKGAHYWRFPKNSIKTEPDAPQPMGPNWLDCPAPSSGPRAPRPPKATPVSETCDCQCELNQAAGRWPAPIPLLLLPLLVGGVASR

Target ORF information:

RefSeq Version	NM_022468.4
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 25 (MMP25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022468.4

ORF Insert Sequence:

ATGCGGCTGC GGCTCCGGCT TCTGGCGCTG CTGCTTCTGC TGCTGGCACC GCCCGCGCGC 
GCCCCGAAGC CCTCGGCGCA GGACGTGAGC CTGGGCGTGG ACTGGCTGAC TCGCTATGGT 
TACCTGCCGC CACCCCACCC TGCCCAGGCC CAGCTGCAGA GCCCTGAGAA GTTGCGCGAT 
GCCATCAAAG TCATGCAGAG GTTCGCGGGG CTGCCGGAGA CCGGCCGCAT GGACCCAGGG 
ACAGTGGCCA CCATGCGTAA GCCCCGCTGC TCCCTGCCTG ACGTGCTGGG GGTGGCGGGG 
CTGGTCAGGC GGCGTCGCCG GTACGCTCTG AGCGGCAGCG TGTGGAAGAA GCGAACCCTG 
ACATGGAGGG TACGTTCCTT CCCCCAGAGC TCCCAGCTGA GCCAGGAGAC CGTGCGGGTC 
CTCATGAGCT ATGCCCTGAT GGCCTGGGGC ATGGAGTCAG GCCTCACATT TCATGAGGTG 
GATTCCCCCC AGGGCCAGGA GCCCGACATC CTCATCGACT TTGCCCGCGC CTTCCACCAG 
GACAGCTACC CCTTCGACGG GTTGGGGGGC ACCCTAGCCC ATGCCTTCTT CCCTGGGGAG 
CACCCCATCT CCGGGGACAC TCACTTTGAC GATGAGGAGA CCTGGACTTT TGGGTCAAAA 
GACGGCGAGG GGACCGACCT GTTTGCCGTG GCTGTCCATG AGTTTGGCCA CGCCCTGGGC 
CTGGGCCACT CCTCAGCCCC CAACTCCATT ATGAGGCCCT TCTACCAGGG TCCGGTGGGC 
GACCCTGACA AGTACCGCCT GTCTCAGGAT GACCGCGATG GCCTGCAGCA ACTCTATGGG 
AAGGCGCCCC AAACCCCATA TGACAAGCCC ACAAGGAAAC CCCTGGCTCC TCCGCCCCAG 
CCCCCGGCCT CGCCCACACA CAGCCCATCC TTCCCCATCC CTGATCGATG TGAGGGCAAT 
TTTGACGCCA TCGCCAACAT CCGAGGGGAA ACTTTCTTCT TCAAAGGCCC CTGGTTCTGG 
CGCCTCCAGC CCTCCGGACA GCTGGTGTCC CCGCGACCCG CACGGCTGCA CCGCTTCTGG 
GAGGGGCTGC CCGCCCAGGT GAGGGTGGTG CAGGCCGCCT ATGCTCGGCA CCGAGACGGC 
CGAATCCTCC TCTTTAGCGG GCCCCAGTTC TGGGTGTTCC AGGACCGGCA GCTGGAGGGC 
GGGGCGCGGC CGCTCACGGA GCTGGGGCTG CCCCCGGGAG AGGAGGTGGA CGCCGTGTTC 
TCGTGGCCAC AGAACGGGAA GACCTACCTG GTCCGCGGCC GGCAGTACTG GCGCTACGAC 
GAGGCGGCGG CGCGCCCGGA CCCCGGCTAC CCTCGCGACC TGAGCCTCTG GGAAGGCGCG 
CCCCCCTCCC CTGACGATGT CACCGTCAGC AACGCAGGTG ACACCTACTT CTTCAAGGGC 
GCCCACTACT GGCGCTTCCC CAAGAACAGC ATCAAGACCG AGCCGGACGC CCCCCAGCCC 
ATGGGGCCCA ACTGGCTGGA CTGCCCCGCC CCGAGCTCTG GTCCCCGCGC CCCCAGGCCC 
CCCAAAGCGA CCCCCGTGTC CGAAACCTGC GATTGTCAGT GCGAGCTCAA CCAGGCCGCA 
GGACGTTGGC CTGCTCCCAT CCCGCTGCTC CTCTTGCCCC TGCTGGTGGG GGGTGTAGCC 
TCCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMP25 ( NM_022468.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071913.1 Homo sapiens matrix metallopeptidase 25 (MMP25), mRNA.

Price & Availability↑

CloneID	OHu13269
Clone ID Related Accession (Same CDS sequence)	NM_022468.4 , NM_022468.5
Accession Version	NM_022468.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	matrix metalloproteinase-25 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC108134.3, AJ272137.1 and CB529891.1. On Nov 22, 2018 this sequence version replaced NM_022468.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ272137.1, AB042328.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000336577.9/ ENSP00000337816.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGCTGC GGCTCCGGCT TCTGGCGCTG CTGCTTCTGC TGCTGGCACC GCCCGCGCGC 
GCCCCGAAGC CCTCGGCGCA GGACGTGAGC CTGGGCGTGG ACTGGCTGAC TCGCTATGGT 
TACCTGCCGC CACCCCACCC TGCCCAGGCC CAGCTGCAGA GCCCTGAGAA GTTGCGCGAT 
GCCATCAAAG TCATGCAGAG GTTCGCGGGG CTGCCGGAGA CCGGCCGCAT GGACCCAGGG 
ACAGTGGCCA CCATGCGTAA GCCCCGCTGC TCCCTGCCTG ACGTGCTGGG GGTGGCGGGG 
CTGGTCAGGC GGCGTCGCCG GTACGCTCTG AGCGGCAGCG TGTGGAAGAA GCGAACCCTG 
ACATGGAGGG TACGTTCCTT CCCCCAGAGC TCCCAGCTGA GCCAGGAGAC CGTGCGGGTC 
CTCATGAGCT ATGCCCTGAT GGCCTGGGGC ATGGAGTCAG GCCTCACATT TCATGAGGTG 
GATTCCCCCC AGGGCCAGGA GCCCGACATC CTCATCGACT TTGCCCGCGC CTTCCACCAG 
GACAGCTACC CCTTCGACGG GTTGGGGGGC ACCCTAGCCC ATGCCTTCTT CCCTGGGGAG 
CACCCCATCT CCGGGGACAC TCACTTTGAC GATGAGGAGA CCTGGACTTT TGGGTCAAAA 
GACGGCGAGG GGACCGACCT GTTTGCCGTG GCTGTCCATG AGTTTGGCCA CGCCCTGGGC 
CTGGGCCACT CCTCAGCCCC CAACTCCATT ATGAGGCCCT TCTACCAGGG TCCGGTGGGC 
GACCCTGACA AGTACCGCCT GTCTCAGGAT GACCGCGATG GCCTGCAGCA ACTCTATGGG 
AAGGCGCCCC AAACCCCATA TGACAAGCCC ACAAGGAAAC CCCTGGCTCC TCCGCCCCAG 
CCCCCGGCCT CGCCCACACA CAGCCCATCC TTCCCCATCC CTGATCGATG TGAGGGCAAT 
TTTGACGCCA TCGCCAACAT CCGAGGGGAA ACTTTCTTCT TCAAAGGCCC CTGGTTCTGG 
CGCCTCCAGC CCTCCGGACA GCTGGTGTCC CCGCGACCCG CACGGCTGCA CCGCTTCTGG 
GAGGGGCTGC CCGCCCAGGT GAGGGTGGTG CAGGCCGCCT ATGCTCGGCA CCGAGACGGC 
CGAATCCTCC TCTTTAGCGG GCCCCAGTTC TGGGTGTTCC AGGACCGGCA GCTGGAGGGC 
GGGGCGCGGC CGCTCACGGA GCTGGGGCTG CCCCCGGGAG AGGAGGTGGA CGCCGTGTTC 
TCGTGGCCAC AGAACGGGAA GACCTACCTG GTCCGCGGCC GGCAGTACTG GCGCTACGAC 
GAGGCGGCGG CGCGCCCGGA CCCCGGCTAC CCTCGCGACC TGAGCCTCTG GGAAGGCGCG 
CCCCCCTCCC CTGACGATGT CACCGTCAGC AACGCAGGTG ACACCTACTT CTTCAAGGGC 
GCCCACTACT GGCGCTTCCC CAAGAACAGC ATCAAGACCG AGCCGGACGC CCCCCAGCCC 
ATGGGGCCCA ACTGGCTGGA CTGCCCCGCC CCGAGCTCTG GTCCCCGCGC CCCCAGGCCC 
CCCAAAGCGA CCCCCGTGTC CGAAACCTGC GATTGTCAGT GCGAGCTCAA CCAGGCCGCA 
GGACGTTGGC CTGCTCCCAT CCCGCTGCTC CTCTTGCCCC TGCTGGTGGG GGGTGTAGCC 
TCCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071913.1
CDS	358..2046
Translation	MRLRLRLLALLLLLLAPPARAPKPSAQDVSLGVDWLTRYGYLPPPHPAQAQLQSPEKLRDAIKVMQRFAGLPETGRMDPGTVATMRKPRCSLPDVLGVAGLVRRRRRYALSGSVWKKRTLTWRVRSFPQSSQLSQETVRVLMSYALMAWGMESGLTFHEVDSPQGQEPDILIDFARAFHQDSYPFDGLGGTLAHAFFPGEHPISGDTHFDDEETWTFGSKDGEGTDLFAVAVHEFGHALGLGHSSAPNSIMRPFYQGPVGDPDKYRLSQDDRDGLQQLYGKAPQTPYDKPTRKPLAPPPQPPASPTHSPSFPIPDRCEGNFDAIANIRGETFFFKGPWFWRLQPSGQLVSPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGGARPLTELGLPPGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLSLWEGAPPSPDDVTVSNAGDTYFFKGAHYWRFPKNSIKTEPDAPQPMGPNWLDCPAPSSGPRAPRPPKATPVSETCDCQCELNQAAGRWPAPIPLLLLPLLVGGVASR

Target ORF information:

RefSeq Version	NM_022468.5
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 25 (MMP25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022468.5

ORF Insert Sequence:

ATGCGGCTGC GGCTCCGGCT TCTGGCGCTG CTGCTTCTGC TGCTGGCACC GCCCGCGCGC 
GCCCCGAAGC CCTCGGCGCA GGACGTGAGC CTGGGCGTGG ACTGGCTGAC TCGCTATGGT 
TACCTGCCGC CACCCCACCC TGCCCAGGCC CAGCTGCAGA GCCCTGAGAA GTTGCGCGAT 
GCCATCAAAG TCATGCAGAG GTTCGCGGGG CTGCCGGAGA CCGGCCGCAT GGACCCAGGG 
ACAGTGGCCA CCATGCGTAA GCCCCGCTGC TCCCTGCCTG ACGTGCTGGG GGTGGCGGGG 
CTGGTCAGGC GGCGTCGCCG GTACGCTCTG AGCGGCAGCG TGTGGAAGAA GCGAACCCTG 
ACATGGAGGG TACGTTCCTT CCCCCAGAGC TCCCAGCTGA GCCAGGAGAC CGTGCGGGTC 
CTCATGAGCT ATGCCCTGAT GGCCTGGGGC ATGGAGTCAG GCCTCACATT TCATGAGGTG 
GATTCCCCCC AGGGCCAGGA GCCCGACATC CTCATCGACT TTGCCCGCGC CTTCCACCAG 
GACAGCTACC CCTTCGACGG GTTGGGGGGC ACCCTAGCCC ATGCCTTCTT CCCTGGGGAG 
CACCCCATCT CCGGGGACAC TCACTTTGAC GATGAGGAGA CCTGGACTTT TGGGTCAAAA 
GACGGCGAGG GGACCGACCT GTTTGCCGTG GCTGTCCATG AGTTTGGCCA CGCCCTGGGC 
CTGGGCCACT CCTCAGCCCC CAACTCCATT ATGAGGCCCT TCTACCAGGG TCCGGTGGGC 
GACCCTGACA AGTACCGCCT GTCTCAGGAT GACCGCGATG GCCTGCAGCA ACTCTATGGG 
AAGGCGCCCC AAACCCCATA TGACAAGCCC ACAAGGAAAC CCCTGGCTCC TCCGCCCCAG 
CCCCCGGCCT CGCCCACACA CAGCCCATCC TTCCCCATCC CTGATCGATG TGAGGGCAAT 
TTTGACGCCA TCGCCAACAT CCGAGGGGAA ACTTTCTTCT TCAAAGGCCC CTGGTTCTGG 
CGCCTCCAGC CCTCCGGACA GCTGGTGTCC CCGCGACCCG CACGGCTGCA CCGCTTCTGG 
GAGGGGCTGC CCGCCCAGGT GAGGGTGGTG CAGGCCGCCT ATGCTCGGCA CCGAGACGGC 
CGAATCCTCC TCTTTAGCGG GCCCCAGTTC TGGGTGTTCC AGGACCGGCA GCTGGAGGGC 
GGGGCGCGGC CGCTCACGGA GCTGGGGCTG CCCCCGGGAG AGGAGGTGGA CGCCGTGTTC 
TCGTGGCCAC AGAACGGGAA GACCTACCTG GTCCGCGGCC GGCAGTACTG GCGCTACGAC 
GAGGCGGCGG CGCGCCCGGA CCCCGGCTAC CCTCGCGACC TGAGCCTCTG GGAAGGCGCG 
CCCCCCTCCC CTGACGATGT CACCGTCAGC AACGCAGGTG ACACCTACTT CTTCAAGGGC 
GCCCACTACT GGCGCTTCCC CAAGAACAGC ATCAAGACCG AGCCGGACGC CCCCCAGCCC 
ATGGGGCCCA ACTGGCTGGA CTGCCCCGCC CCGAGCTCTG GTCCCCGCGC CCCCAGGCCC 
CCCAAAGCGA CCCCCGTGTC CGAAACCTGC GATTGTCAGT GCGAGCTCAA CCAGGCCGCA 
GGACGTTGGC CTGCTCCCAT CCCGCTGCTC CTCTTGCCCC TGCTGGTGGG GGGTGTAGCC 
TCCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMP25 ( XM_024450391.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306159.1 Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu71364
Clone ID Related Accession (Same CDS sequence)	XM_024450391.1 , XM_011522602.2
Accession Version	XM_024450391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	matrix metalloproteinase-25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAT GTGGAAGAAG AGTGTCCCGG GCAAGTGCAA AGACCCCACG GCACGCAGAA 
GCTTGTGTGC CTGGAGCAAA AGGAGCATTG AAGAGCTCTG GCGAGAGGCC TCAGCCAAGG 
GCCCTGAAAG ACCCAGGGAC AGTGGCCACC ATGCGTAAGC CCCGCTGCTC CCTGCCTGAC 
GTGCTGGGGG TGGCGGGGCT GGTCAGGCGG CGTCGCCGGT ACGCTCTGAG CGGCAGCGTG 
TGGAAGAAGC GAACCCTGAC ATGGAGGGTA CGTTCCTTCC CCCAGAGCTC CCAGCTGAGC 
CAGGAGACCG TGCGGGTCCT CATGAGCTAT GCCCTGATGG CCTGGGGCAT GGAGTCAGGC 
CTCACATTTC ATGAGGTGGA TTCCCCCCAG GGCCAGGAGC CCGACATCCT CATCGACTTT 
GCCCGCGCCT TCCACCAGGA CAGCTACCCC TTCGACGGGT TGGGGGGCAC CCTAGCCCAT 
GCCTTCTTCC CTGGGGAGCA CCCCATCTCC GGGGACACTC ACTTTGACGA TGAGGAGACC 
TGGACTTTTG GGTCAAAAGA CGGCGAGGGG ACCGACCTGT TTGCCGTGGC TGTCCATGAG 
TTTGGCCACG CCCTGGGCCT GGGCCACTCC TCAGCCCCCA ACTCCATTAT GAGGCCCTTC 
TACCAGGGTC CGGTGGGCGA CCCTGACAAG TACCGCCTGT CTCAGGATGA CCGCGATGGC 
CTGCAGCAAC TCTATGGGAA GGCGCCCCAA ACCCCATATG ACAAGCCCAC AAGGAAACCC 
CTGGCTCCTC CGCCCCAGCC CCCGGCCTCG CCCACACACA GCCCATCCTT CCCCATCCCT 
GATCGATGTG AGGGCAATTT TGACGCCATC GCCAACATCC GAGGGGAAAC TTTCTTCTTC 
AAAGGCCCCT GGTTCTGGCG CCTCCAGCCC TCCGGACAGC TGGTGTCCCC GCGACCCGCA 
CGGCTGCACC GCTTCTGGGA GGGGCTGCCC GCCCAGGTGA GGGTGGTGCA GGCCGCCTAT 
GCTCGGCACC GAGACGGCCG AATCCTCCTC TTTAGCGGGC CCCAGTTCTG GGTGTTCCAG 
GACCGGCAGC TGGAGGGCGG GGCGCGGCCG CTCACGGAGC TGGGGCTGCC CCCGGGAGAG 
GAGGTGGACG CCGTGTTCTC GTGGCCACAG AACGGGAAGA CCTACCTGGT CCGCGGCCGG 
CAGTACTGGC GCTACGACGA GGCGGCGGCG CGCCCGGACC CCGGCTACCC TCGCGACCTG 
AGCCTCTGGG AAGGCGCGCC CCCCTCCCCT GACGATGTCA CCGTCAGCAA CGCAGGTGAC 
ACCTACTTCT TCAAGGGCGC CCACTACTGG CGCTTCCCCA AGAACAGCAT CAAGACCGAG 
CCGGACGCCC CCCAGCCCAT GGGGCCCAAC TGGCTGGACT GCCCCGCCCC GAGCTCTGGT 
CCCCGCGCCC CCAGGCCCCC CAAAGCGACC CCCGTGTCCG AAACCTGCGA TTGTCAGTGC 
GAGCTCAACC AGGCCGCAGG ACGTTGGCCT GCTCCCATCC CGCTGCTCCT CTTGCCCCTG 
CTGGTGGGGG GTGTAGCCTC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306159.1
CDS	357..1943
Translation	MGRCGRRVSRASAKTPRHAEACVPGAKGALKSSGERPQPRALKDPGTVATMRKPRCSLPDVLGVAGLVRRRRRYALSGSVWKKRTLTWRVRSFPQSSQLSQETVRVLMSYALMAWGMESGLTFHEVDSPQGQEPDILIDFARAFHQDSYPFDGLGGTLAHAFFPGEHPISGDTHFDDEETWTFGSKDGEGTDLFAVAVHEFGHALGLGHSSAPNSIMRPFYQGPVGDPDKYRLSQDDRDGLQQLYGKAPQTPYDKPTRKPLAPPPQPPASPTHSPSFPIPDRCEGNFDAIANIRGETFFFKGPWFWRLQPSGQLVSPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGGARPLTELGLPPGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLSLWEGAPPSPDDVTVSNAGDTYFFKGAHYWRFPKNSIKTEPDAPQPMGPNWLDCPAPSSGPRAPRPPKATPVSETCDCQCELNQAAGRWPAPIPLLLLPLLVGGVASR

Target ORF information:

RefSeq Version	XM_024450391.1
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450391.1

ORF Insert Sequence:

ATGGGAAGAT GTGGAAGAAG AGTGTCCCGG GCAAGTGCAA AGACCCCACG GCACGCAGAA 
GCTTGTGTGC CTGGAGCAAA AGGAGCATTG AAGAGCTCTG GCGAGAGGCC TCAGCCAAGG 
GCCCTGAAAG ACCCAGGGAC AGTGGCCACC ATGCGTAAGC CCCGCTGCTC CCTGCCTGAC 
GTGCTGGGGG TGGCGGGGCT GGTCAGGCGG CGTCGCCGGT ACGCTCTGAG CGGCAGCGTG 
TGGAAGAAGC GAACCCTGAC ATGGAGGGTA CGTTCCTTCC CCCAGAGCTC CCAGCTGAGC 
CAGGAGACCG TGCGGGTCCT CATGAGCTAT GCCCTGATGG CCTGGGGCAT GGAGTCAGGC 
CTCACATTTC ATGAGGTGGA TTCCCCCCAG GGCCAGGAGC CCGACATCCT CATCGACTTT 
GCCCGCGCCT TCCACCAGGA CAGCTACCCC TTCGACGGGT TGGGGGGCAC CCTAGCCCAT 
GCCTTCTTCC CTGGGGAGCA CCCCATCTCC GGGGACACTC ACTTTGACGA TGAGGAGACC 
TGGACTTTTG GGTCAAAAGA CGGCGAGGGG ACCGACCTGT TTGCCGTGGC TGTCCATGAG 
TTTGGCCACG CCCTGGGCCT GGGCCACTCC TCAGCCCCCA ACTCCATTAT GAGGCCCTTC 
TACCAGGGTC CGGTGGGCGA CCCTGACAAG TACCGCCTGT CTCAGGATGA CCGCGATGGC 
CTGCAGCAAC TCTATGGGAA GGCGCCCCAA ACCCCATATG ACAAGCCCAC AAGGAAACCC 
CTGGCTCCTC CGCCCCAGCC CCCGGCCTCG CCCACACACA GCCCATCCTT CCCCATCCCT 
GATCGATGTG AGGGCAATTT TGACGCCATC GCCAACATCC GAGGGGAAAC TTTCTTCTTC 
AAAGGCCCCT GGTTCTGGCG CCTCCAGCCC TCCGGACAGC TGGTGTCCCC GCGACCCGCA 
CGGCTGCACC GCTTCTGGGA GGGGCTGCCC GCCCAGGTGA GGGTGGTGCA GGCCGCCTAT 
GCTCGGCACC GAGACGGCCG AATCCTCCTC TTTAGCGGGC CCCAGTTCTG GGTGTTCCAG 
GACCGGCAGC TGGAGGGCGG GGCGCGGCCG CTCACGGAGC TGGGGCTGCC CCCGGGAGAG 
GAGGTGGACG CCGTGTTCTC GTGGCCACAG AACGGGAAGA CCTACCTGGT CCGCGGCCGG 
CAGTACTGGC GCTACGACGA GGCGGCGGCG CGCCCGGACC CCGGCTACCC TCGCGACCTG 
AGCCTCTGGG AAGGCGCGCC CCCCTCCCCT GACGATGTCA CCGTCAGCAA CGCAGGTGAC 
ACCTACTTCT TCAAGGGCGC CCACTACTGG CGCTTCCCCA AGAACAGCAT CAAGACCGAG 
CCGGACGCCC CCCAGCCCAT GGGGCCCAAC TGGCTGGACT GCCCCGCCCC GAGCTCTGGT 
CCCCGCGCCC CCAGGCCCCC CAAAGCGACC CCCGTGTCCG AAACCTGCGA TTGTCAGTGC 
GAGCTCAACC AGGCCGCAGG ACGTTGGCCT GCTCCCATCC CGCTGCTCCT CTTGCCCCTG 
CTGGTGGGGG GTGTAGCCTC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMP25 ( XM_011522602.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520904.1 Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu71364
Clone ID Related Accession (Same CDS sequence)	XM_024450391.1 , XM_011522602.2
Accession Version	XM_011522602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	matrix metalloproteinase-25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAT GTGGAAGAAG AGTGTCCCGG GCAAGTGCAA AGACCCCACG GCACGCAGAA 
GCTTGTGTGC CTGGAGCAAA AGGAGCATTG AAGAGCTCTG GCGAGAGGCC TCAGCCAAGG 
GCCCTGAAAG ACCCAGGGAC AGTGGCCACC ATGCGTAAGC CCCGCTGCTC CCTGCCTGAC 
GTGCTGGGGG TGGCGGGGCT GGTCAGGCGG CGTCGCCGGT ACGCTCTGAG CGGCAGCGTG 
TGGAAGAAGC GAACCCTGAC ATGGAGGGTA CGTTCCTTCC CCCAGAGCTC CCAGCTGAGC 
CAGGAGACCG TGCGGGTCCT CATGAGCTAT GCCCTGATGG CCTGGGGCAT GGAGTCAGGC 
CTCACATTTC ATGAGGTGGA TTCCCCCCAG GGCCAGGAGC CCGACATCCT CATCGACTTT 
GCCCGCGCCT TCCACCAGGA CAGCTACCCC TTCGACGGGT TGGGGGGCAC CCTAGCCCAT 
GCCTTCTTCC CTGGGGAGCA CCCCATCTCC GGGGACACTC ACTTTGACGA TGAGGAGACC 
TGGACTTTTG GGTCAAAAGA CGGCGAGGGG ACCGACCTGT TTGCCGTGGC TGTCCATGAG 
TTTGGCCACG CCCTGGGCCT GGGCCACTCC TCAGCCCCCA ACTCCATTAT GAGGCCCTTC 
TACCAGGGTC CGGTGGGCGA CCCTGACAAG TACCGCCTGT CTCAGGATGA CCGCGATGGC 
CTGCAGCAAC TCTATGGGAA GGCGCCCCAA ACCCCATATG ACAAGCCCAC AAGGAAACCC 
CTGGCTCCTC CGCCCCAGCC CCCGGCCTCG CCCACACACA GCCCATCCTT CCCCATCCCT 
GATCGATGTG AGGGCAATTT TGACGCCATC GCCAACATCC GAGGGGAAAC TTTCTTCTTC 
AAAGGCCCCT GGTTCTGGCG CCTCCAGCCC TCCGGACAGC TGGTGTCCCC GCGACCCGCA 
CGGCTGCACC GCTTCTGGGA GGGGCTGCCC GCCCAGGTGA GGGTGGTGCA GGCCGCCTAT 
GCTCGGCACC GAGACGGCCG AATCCTCCTC TTTAGCGGGC CCCAGTTCTG GGTGTTCCAG 
GACCGGCAGC TGGAGGGCGG GGCGCGGCCG CTCACGGAGC TGGGGCTGCC CCCGGGAGAG 
GAGGTGGACG CCGTGTTCTC GTGGCCACAG AACGGGAAGA CCTACCTGGT CCGCGGCCGG 
CAGTACTGGC GCTACGACGA GGCGGCGGCG CGCCCGGACC CCGGCTACCC TCGCGACCTG 
AGCCTCTGGG AAGGCGCGCC CCCCTCCCCT GACGATGTCA CCGTCAGCAA CGCAGGTGAC 
ACCTACTTCT TCAAGGGCGC CCACTACTGG CGCTTCCCCA AGAACAGCAT CAAGACCGAG 
CCGGACGCCC CCCAGCCCAT GGGGCCCAAC TGGCTGGACT GCCCCGCCCC GAGCTCTGGT 
CCCCGCGCCC CCAGGCCCCC CAAAGCGACC CCCGTGTCCG AAACCTGCGA TTGTCAGTGC 
GAGCTCAACC AGGCCGCAGG ACGTTGGCCT GCTCCCATCC CGCTGCTCCT CTTGCCCCTG 
CTGGTGGGGG GTGTAGCCTC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520904.1
CDS	77..1663
Translation	MGRCGRRVSRASAKTPRHAEACVPGAKGALKSSGERPQPRALKDPGTVATMRKPRCSLPDVLGVAGLVRRRRRYALSGSVWKKRTLTWRVRSFPQSSQLSQETVRVLMSYALMAWGMESGLTFHEVDSPQGQEPDILIDFARAFHQDSYPFDGLGGTLAHAFFPGEHPISGDTHFDDEETWTFGSKDGEGTDLFAVAVHEFGHALGLGHSSAPNSIMRPFYQGPVGDPDKYRLSQDDRDGLQQLYGKAPQTPYDKPTRKPLAPPPQPPASPTHSPSFPIPDRCEGNFDAIANIRGETFFFKGPWFWRLQPSGQLVSPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGGARPLTELGLPPGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLSLWEGAPPSPDDVTVSNAGDTYFFKGAHYWRFPKNSIKTEPDAPQPMGPNWLDCPAPSSGPRAPRPPKATPVSETCDCQCELNQAAGRWPAPIPLLLLPLLVGGVASR

Target ORF information:

RefSeq Version	XM_011522602.2
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522602.2

ORF Insert Sequence:

ATGGGAAGAT GTGGAAGAAG AGTGTCCCGG GCAAGTGCAA AGACCCCACG GCACGCAGAA 
GCTTGTGTGC CTGGAGCAAA AGGAGCATTG AAGAGCTCTG GCGAGAGGCC TCAGCCAAGG 
GCCCTGAAAG ACCCAGGGAC AGTGGCCACC ATGCGTAAGC CCCGCTGCTC CCTGCCTGAC 
GTGCTGGGGG TGGCGGGGCT GGTCAGGCGG CGTCGCCGGT ACGCTCTGAG CGGCAGCGTG 
TGGAAGAAGC GAACCCTGAC ATGGAGGGTA CGTTCCTTCC CCCAGAGCTC CCAGCTGAGC 
CAGGAGACCG TGCGGGTCCT CATGAGCTAT GCCCTGATGG CCTGGGGCAT GGAGTCAGGC 
CTCACATTTC ATGAGGTGGA TTCCCCCCAG GGCCAGGAGC CCGACATCCT CATCGACTTT 
GCCCGCGCCT TCCACCAGGA CAGCTACCCC TTCGACGGGT TGGGGGGCAC CCTAGCCCAT 
GCCTTCTTCC CTGGGGAGCA CCCCATCTCC GGGGACACTC ACTTTGACGA TGAGGAGACC 
TGGACTTTTG GGTCAAAAGA CGGCGAGGGG ACCGACCTGT TTGCCGTGGC TGTCCATGAG 
TTTGGCCACG CCCTGGGCCT GGGCCACTCC TCAGCCCCCA ACTCCATTAT GAGGCCCTTC 
TACCAGGGTC CGGTGGGCGA CCCTGACAAG TACCGCCTGT CTCAGGATGA CCGCGATGGC 
CTGCAGCAAC TCTATGGGAA GGCGCCCCAA ACCCCATATG ACAAGCCCAC AAGGAAACCC 
CTGGCTCCTC CGCCCCAGCC CCCGGCCTCG CCCACACACA GCCCATCCTT CCCCATCCCT 
GATCGATGTG AGGGCAATTT TGACGCCATC GCCAACATCC GAGGGGAAAC TTTCTTCTTC 
AAAGGCCCCT GGTTCTGGCG CCTCCAGCCC TCCGGACAGC TGGTGTCCCC GCGACCCGCA 
CGGCTGCACC GCTTCTGGGA GGGGCTGCCC GCCCAGGTGA GGGTGGTGCA GGCCGCCTAT 
GCTCGGCACC GAGACGGCCG AATCCTCCTC TTTAGCGGGC CCCAGTTCTG GGTGTTCCAG 
GACCGGCAGC TGGAGGGCGG GGCGCGGCCG CTCACGGAGC TGGGGCTGCC CCCGGGAGAG 
GAGGTGGACG CCGTGTTCTC GTGGCCACAG AACGGGAAGA CCTACCTGGT CCGCGGCCGG 
CAGTACTGGC GCTACGACGA GGCGGCGGCG CGCCCGGACC CCGGCTACCC TCGCGACCTG 
AGCCTCTGGG AAGGCGCGCC CCCCTCCCCT GACGATGTCA CCGTCAGCAA CGCAGGTGAC 
ACCTACTTCT TCAAGGGCGC CCACTACTGG CGCTTCCCCA AGAACAGCAT CAAGACCGAG 
CCGGACGCCC CCCAGCCCAT GGGGCCCAAC TGGCTGGACT GCCCCGCCCC GAGCTCTGGT 
CCCCGCGCCC CCAGGCCCCC CAAAGCGACC CCCGTGTCCG AAACCTGCGA TTGTCAGTGC 
GAGCTCAACC AGGCCGCAGG ACGTTGGCCT GCTCCCATCC CGCTGCTCCT CTTGCCCCTG 
CTGGTGGGGG GTGTAGCCTC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMP25 ( XM_011522604.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520906.1 Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu71366
Clone ID Related Accession (Same CDS sequence)	XM_011522604.2
Accession Version	XM_011522604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	matrix metalloproteinase-25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTAAGC CCCGCTGCTC CCTGCCTGAC GTGCTGGGGG TGGCGGGGCT GGTCAGGCGG 
CGTCGCCGGT ACGCTCTGAG CGGCAGCGTG TGGAAGAAGC GAACCCTGAC ATGGAGGGTA 
CGTTCCTTCC CCCAGAGCTC CCAGCTGAGC CAGGAGACCG TGCGGGTCCT CATGAGCTAT 
GCCCTGATGG CCTGGGGCAT GGAGTCAGGC CTCACATTTC ATGAGGTGGA TTCCCCCCAG 
GGCCAGGAGC CCGACATCCT CATCGACTTT GCCCGCGCCT TCCACCAGGA CAGCTACCCC 
TTCGACGGGT TGGGGGGCAC CCTAGCCCAT GCCTTCTTCC CTGGGGAGCA CCCCATCTCC 
GGGGACACTC ACTTTGACGA TGAGGAGACC TGGACTTTTG GGTCAAAAGA CGGCGAGGGG 
ACCGACCTGT TTGCCGTGGC TGTCCATGAG TTTGGCCACG CCCTGGGCCT GGGCCACTCC 
TCAGCCCCCA ACTCCATTAT GAGGCCCTTC TACCAGGGTC CGGTGGGCGA CCCTGACAAG 
TACCGCCTGT CTCAGGATGA CCGCGATGGC CTGCAGCAAC TCTATGGGAA GGCGCCCCAA 
ACCCCATATG ACAAGCCCAC AAGGAAACCC CTGGCTCCTC CGCCCCAGCC CCCGGCCTCG 
CCCACACACA GCCCATCCTT CCCCATCCCT GATCGATGTG AGGGCAATTT TGACGCCATC 
GCCAACATCC GAGGGGAAAC TTTCTTCTTC AAAGGCCCCT GGTTCTGGCG CCTCCAGCCC 
TCCGGACAGC TGGTGTCCCC GCGACCCGCA CGGCTGCACC GCTTCTGGGA GGGGCTGCCC 
GCCCAGGTGA GGGTGGTGCA GGCCGCCTAT GCTCGGCACC GAGACGGCCG AATCCTCCTC 
TTTAGCGGGC CCCAGTTCTG GGTGTTCCAG GACCGGCAGC TGGAGGGCGG GGCGCGGCCG 
CTCACGGAGC TGGGGCTGCC CCCGGGAGAG GAGGTGGACG CCGTGTTCTC GTGGCCACAG 
AACGGGAAGA CCTACCTGGT CCGCGGCCGG CAGTACTGGC GCTACGACGA GGCGGCGGCG 
CGCCCGGACC CCGGCTACCC TCGCGACCTG AGCCTCTGGG AAGGCGCGCC CCCCTCCCCT 
GACGATGTCA CCGTCAGCAA CGCAGGTGAC ACCTACTTCT TCAAGGGCGC CCACTACTGG 
CGCTTCCCCA AGAACAGCAT CAAGACCGAG CCGGACGCCC CCCAGCCCAT GGGGCCCAAC 
TGGCTGGACT GCCCCGCCCC GAGCTCTGGT CCCCGCGCCC CCAGGCCCCC CAAAGCGACC 
CCCGTGTCCG AAACCTGCGA TTGTCAGTGC GAGCTCAACC AGGCCGCAGG ACGTTGGCCT 
GCTCCCATCC CGCTGCTCCT CTTGCCCCTG CTGGTGGGGG GTGTAGCCTC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520906.1
CDS	341..1777
Translation	MRKPRCSLPDVLGVAGLVRRRRRYALSGSVWKKRTLTWRVRSFPQSSQLSQETVRVLMSYALMAWGMESGLTFHEVDSPQGQEPDILIDFARAFHQDSYPFDGLGGTLAHAFFPGEHPISGDTHFDDEETWTFGSKDGEGTDLFAVAVHEFGHALGLGHSSAPNSIMRPFYQGPVGDPDKYRLSQDDRDGLQQLYGKAPQTPYDKPTRKPLAPPPQPPASPTHSPSFPIPDRCEGNFDAIANIRGETFFFKGPWFWRLQPSGQLVSPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGGARPLTELGLPPGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLSLWEGAPPSPDDVTVSNAGDTYFFKGAHYWRFPKNSIKTEPDAPQPMGPNWLDCPAPSSGPRAPRPPKATPVSETCDCQCELNQAAGRWPAPIPLLLLPLLVGGVASR

Target ORF information:

RefSeq Version	XM_011522604.2
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522604.2

ORF Insert Sequence:

ATGCGTAAGC CCCGCTGCTC CCTGCCTGAC GTGCTGGGGG TGGCGGGGCT GGTCAGGCGG 
CGTCGCCGGT ACGCTCTGAG CGGCAGCGTG TGGAAGAAGC GAACCCTGAC ATGGAGGGTA 
CGTTCCTTCC CCCAGAGCTC CCAGCTGAGC CAGGAGACCG TGCGGGTCCT CATGAGCTAT 
GCCCTGATGG CCTGGGGCAT GGAGTCAGGC CTCACATTTC ATGAGGTGGA TTCCCCCCAG 
GGCCAGGAGC CCGACATCCT CATCGACTTT GCCCGCGCCT TCCACCAGGA CAGCTACCCC 
TTCGACGGGT TGGGGGGCAC CCTAGCCCAT GCCTTCTTCC CTGGGGAGCA CCCCATCTCC 
GGGGACACTC ACTTTGACGA TGAGGAGACC TGGACTTTTG GGTCAAAAGA CGGCGAGGGG 
ACCGACCTGT TTGCCGTGGC TGTCCATGAG TTTGGCCACG CCCTGGGCCT GGGCCACTCC 
TCAGCCCCCA ACTCCATTAT GAGGCCCTTC TACCAGGGTC CGGTGGGCGA CCCTGACAAG 
TACCGCCTGT CTCAGGATGA CCGCGATGGC CTGCAGCAAC TCTATGGGAA GGCGCCCCAA 
ACCCCATATG ACAAGCCCAC AAGGAAACCC CTGGCTCCTC CGCCCCAGCC CCCGGCCTCG 
CCCACACACA GCCCATCCTT CCCCATCCCT GATCGATGTG AGGGCAATTT TGACGCCATC 
GCCAACATCC GAGGGGAAAC TTTCTTCTTC AAAGGCCCCT GGTTCTGGCG CCTCCAGCCC 
TCCGGACAGC TGGTGTCCCC GCGACCCGCA CGGCTGCACC GCTTCTGGGA GGGGCTGCCC 
GCCCAGGTGA GGGTGGTGCA GGCCGCCTAT GCTCGGCACC GAGACGGCCG AATCCTCCTC 
TTTAGCGGGC CCCAGTTCTG GGTGTTCCAG GACCGGCAGC TGGAGGGCGG GGCGCGGCCG 
CTCACGGAGC TGGGGCTGCC CCCGGGAGAG GAGGTGGACG CCGTGTTCTC GTGGCCACAG 
AACGGGAAGA CCTACCTGGT CCGCGGCCGG CAGTACTGGC GCTACGACGA GGCGGCGGCG 
CGCCCGGACC CCGGCTACCC TCGCGACCTG AGCCTCTGGG AAGGCGCGCC CCCCTCCCCT 
GACGATGTCA CCGTCAGCAA CGCAGGTGAC ACCTACTTCT TCAAGGGCGC CCACTACTGG 
CGCTTCCCCA AGAACAGCAT CAAGACCGAG CCGGACGCCC CCCAGCCCAT GGGGCCCAAC 
TGGCTGGACT GCCCCGCCCC GAGCTCTGGT CCCCGCGCCC CCAGGCCCCC CAAAGCGACC 
CCCGTGTCCG AAACCTGCGA TTGTCAGTGC GAGCTCAACC AGGCCGCAGG ACGTTGGCCT 
GCTCCCATCC CGCTGCTCCT CTTGCCCCTG CTGGTGGGGG GTGTAGCCTC CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMP25 ( XM_011522605.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520907.1 Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu71367
Clone ID Related Accession (Same CDS sequence)	XM_011522605.2
Accession Version	XM_011522605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	matrix metalloproteinase-25 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGACAAGG CAGGGAAGAA GACGGCGAGG GGACCGACCT GTTTGCCGTG 
GCTGTCCATG AGTTTGGCCA CGCCCTGGGC CTGGGCCACT CCTCAGCCCC CAACTCCATT 
ATGAGGCCCT TCTACCAGGG TCCGGTGGGC GACCCTGACA AGTACCGCCT GTCTCAGGAT 
GACCGCGATG GCCTGCAGCA ACTCTATGGG AAGGCGCCCC AAACCCCATA TGACAAGCCC 
ACAAGGAAAC CCCTGGCTCC TCCGCCCCAG CCCCCGGCCT CGCCCACACA CAGCCCATCC 
TTCCCCATCC CTGATCGATG TGAGGGCAAT TTTGACGCCA TCGCCAACAT CCGAGGGGAA 
ACTTTCTTCT TCAAAGGCCC CTGGTTCTGG CGCCTCCAGC CCTCCGGACA GCTGGTGTCC 
CCGCGACCCG CACGGCTGCA CCGCTTCTGG GAGGGGCTGC CCGCCCAGGT GAGGGTGGTG 
CAGGCCGCCT ATGCTCGGCA CCGAGACGGC CGAATCCTCC TCTTTAGCGG GCCCCAGTTC 
TGGGTGTTCC AGGACCGGCA GCTGGAGGGC GGGGCGCGGC CGCTCACGGA GCTGGGGCTG 
CCCCCGGGAG AGGAGGTGGA CGCCGTGTTC TCGTGGCCAC AGAACGGGAA GACCTACCTG 
GTCCGCGGCC GGCAGTACTG GCGCTACGAC GAGGCGGCGG CGCGCCCGGA CCCCGGCTAC 
CCTCGCGACC TGAGCCTCTG GGAAGGCGCG CCCCCCTCCC CTGACGATGT CACCGTCAGC 
AACGCAGGTG ACACCTACTT CTTCAAGGGC GCCCACTACT GGCGCTTCCC CAAGAACAGC 
ATCAAGACCG AGCCGGACGC CCCCCAGCCC ATGGGGCCCA ACTGGCTGGA CTGCCCCGCC 
CCGAGCTCTG GTCCCCGCGC CCCCAGGCCC CCCAAAGCGA CCCCCGTGTC CGAAACCTGC 
GATTGTCAGT GCGAGCTCAA CCAGGCCGCA GGACGTTGGC CTGCTCCCAT CCCGCTGCTC 
CTCTTGCCCC TGCTGGTGGG GGGTGTAGCC TCCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520907.1
CDS	59..1117
Translation	MAKERQGREEDGEGTDLFAVAVHEFGHALGLGHSSAPNSIMRPFYQGPVGDPDKYRLSQDDRDGLQQLYGKAPQTPYDKPTRKPLAPPPQPPASPTHSPSFPIPDRCEGNFDAIANIRGETFFFKGPWFWRLQPSGQLVSPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGGARPLTELGLPPGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLSLWEGAPPSPDDVTVSNAGDTYFFKGAHYWRFPKNSIKTEPDAPQPMGPNWLDCPAPSSGPRAPRPPKATPVSETCDCQCELNQAAGRWPAPIPLLLLPLLVGGVASR

Target ORF information:

RefSeq Version	XM_011522605.2
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522605.2

ORF Insert Sequence:

ATGGCCAAGG AGAGACAAGG CAGGGAAGAA GACGGCGAGG GGACCGACCT GTTTGCCGTG 
GCTGTCCATG AGTTTGGCCA CGCCCTGGGC CTGGGCCACT CCTCAGCCCC CAACTCCATT 
ATGAGGCCCT TCTACCAGGG TCCGGTGGGC GACCCTGACA AGTACCGCCT GTCTCAGGAT 
GACCGCGATG GCCTGCAGCA ACTCTATGGG AAGGCGCCCC AAACCCCATA TGACAAGCCC 
ACAAGGAAAC CCCTGGCTCC TCCGCCCCAG CCCCCGGCCT CGCCCACACA CAGCCCATCC 
TTCCCCATCC CTGATCGATG TGAGGGCAAT TTTGACGCCA TCGCCAACAT CCGAGGGGAA 
ACTTTCTTCT TCAAAGGCCC CTGGTTCTGG CGCCTCCAGC CCTCCGGACA GCTGGTGTCC 
CCGCGACCCG CACGGCTGCA CCGCTTCTGG GAGGGGCTGC CCGCCCAGGT GAGGGTGGTG 
CAGGCCGCCT ATGCTCGGCA CCGAGACGGC CGAATCCTCC TCTTTAGCGG GCCCCAGTTC 
TGGGTGTTCC AGGACCGGCA GCTGGAGGGC GGGGCGCGGC CGCTCACGGA GCTGGGGCTG 
CCCCCGGGAG AGGAGGTGGA CGCCGTGTTC TCGTGGCCAC AGAACGGGAA GACCTACCTG 
GTCCGCGGCC GGCAGTACTG GCGCTACGAC GAGGCGGCGG CGCGCCCGGA CCCCGGCTAC 
CCTCGCGACC TGAGCCTCTG GGAAGGCGCG CCCCCCTCCC CTGACGATGT CACCGTCAGC 
AACGCAGGTG ACACCTACTT CTTCAAGGGC GCCCACTACT GGCGCTTCCC CAAGAACAGC 
ATCAAGACCG AGCCGGACGC CCCCCAGCCC ATGGGGCCCA ACTGGCTGGA CTGCCCCGCC 
CCGAGCTCTG GTCCCCGCGC CCCCAGGCCC CCCAAAGCGA CCCCCGTGTC CGAAACCTGC 
GATTGTCAGT GCGAGCTCAA CCAGGCCGCA GGACGTTGGC CTGCTCCCAT CCCGCTGCTC 
CTCTTGCCCC TGCTGGTGGG GGGTGTAGCC TCCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMP25 ( XM_024450390.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306158.1 Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X1, mRNA.

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CloneID	OHu71363
Clone ID Related Accession (Same CDS sequence)	XM_024450390.1
Accession Version	XM_024450390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	matrix metalloproteinase-25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTGC GGCTCCGGCT TCTGGCGCTG CTGCTTCTGC TGCTGGCACC GCCCGCGCGC 
GCCCCGAAGC CCTCGGCGCA GGACGTGAGC CTGGGCGTGG ACTGGCTGAC TCGCTATGGT 
TACCTGCCGC CACCCCACCC TGCCCAGGCC CAGCTGCAGA GCCCTGAGAA GTTGCGCGAT 
GCCATCAAAG TCATGCAGAG GTTCGCGGGG CTGCCGGAGA CCGGCCGCAT GGACGGCGAG 
GGGACCGACC TGTTTGCCGT GGCTGTCCAT GAGTTTGGCC ACGCCCTGGG CCTGGGCCAC 
TCCTCAGCCC CCAACTCCAT TATGAGGCCC TTCTACCAGG GTCCGGTGGG CGACCCTGAC 
AAGTACCGCC TGTCTCAGGA TGACCGCGAT GGCCTGCAGC AACTCTATGG GAAGGCGCCC 
CAAACCCCAT ATGACAAGCC CACAAGGAAA CCCCTGGCTC CTCCGCCCCA GCCCCCGGCC 
TCGCCCACAC ACAGCCCATC CTTCCCCATC CCTGATCGAT GTGAGGGCAA TTTTGACGCC 
ATCGCCAACA TCCGAGGGGA AACTTTCTTC TTCAAAGGCC CCTGGTTCTG GCGCCTCCAG 
CCCTCCGGAC AGCTGGTGTC CCCGCGACCC GCACGGCTGC ACCGCTTCTG GGAGGGGCTG 
CCCGCCCAGG TGAGGGTGGT GCAGGCCGCC TATGCTCGGC ACCGAGACGG CCGAATCCTC 
CTCTTTAGCG GGCCCCAGTT CTGGGTGTTC CAGGACCGGC AGCTGGAGGG CGGGGCGCGG 
CCGCTCACGG AGCTGGGGCT GCCCCCGGGA GAGGAGGTGG ACGCCGTGTT CTCGTGGCCA 
CAGAACGGGA AGACCTACCT GGTCCGCGGC CGGCAGTACT GGCGCTACGA CGAGGCGGCG 
GCGCGCCCGG ACCCCGGCTA CCCTCGCGAC CTGAGCCTCT GGGAAGGCGC GCCCCCCTCC 
CCTGACGATG TCACCGTCAG CAACGCAGGT GACACCTACT TCTTCAAGGG CGCCCACTAC 
TGGCGCTTCC CCAAGAACAG CATCAAGACC GAGCCGGACG CCCCCCAGCC CATGGGGCCC 
AACTGGCTGG ACTGCCCCGC CCCGAGCTCT GGTCCCCGCG CCCCCAGGCC CCCCAAAGCG 
ACCCCCGTGT CCGAAACCTG CGATTGTCAG TGCGAGCTCA ACCAGGCCGC AGGACGTTGG 
CCTGCTCCCA TCCCGCTGCT CCTCTTGCCC CTGCTGGTGG GGGGTGTAGC CTCCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306158.1
CDS	956..2215
Translation	MRLRLRLLALLLLLLAPPARAPKPSAQDVSLGVDWLTRYGYLPPPHPAQAQLQSPEKLRDAIKVMQRFAGLPETGRMDGEGTDLFAVAVHEFGHALGLGHSSAPNSIMRPFYQGPVGDPDKYRLSQDDRDGLQQLYGKAPQTPYDKPTRKPLAPPPQPPASPTHSPSFPIPDRCEGNFDAIANIRGETFFFKGPWFWRLQPSGQLVSPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGGARPLTELGLPPGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLSLWEGAPPSPDDVTVSNAGDTYFFKGAHYWRFPKNSIKTEPDAPQPMGPNWLDCPAPSSGPRAPRPPKATPVSETCDCQCELNQAAGRWPAPIPLLLLPLLVGGVASR

Target ORF information:

RefSeq Version	XM_024450390.1
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450390.1

ORF Insert Sequence:

ATGCGGCTGC GGCTCCGGCT TCTGGCGCTG CTGCTTCTGC TGCTGGCACC GCCCGCGCGC 
GCCCCGAAGC CCTCGGCGCA GGACGTGAGC CTGGGCGTGG ACTGGCTGAC TCGCTATGGT 
TACCTGCCGC CACCCCACCC TGCCCAGGCC CAGCTGCAGA GCCCTGAGAA GTTGCGCGAT 
GCCATCAAAG TCATGCAGAG GTTCGCGGGG CTGCCGGAGA CCGGCCGCAT GGACGGCGAG 
GGGACCGACC TGTTTGCCGT GGCTGTCCAT GAGTTTGGCC ACGCCCTGGG CCTGGGCCAC 
TCCTCAGCCC CCAACTCCAT TATGAGGCCC TTCTACCAGG GTCCGGTGGG CGACCCTGAC 
AAGTACCGCC TGTCTCAGGA TGACCGCGAT GGCCTGCAGC AACTCTATGG GAAGGCGCCC 
CAAACCCCAT ATGACAAGCC CACAAGGAAA CCCCTGGCTC CTCCGCCCCA GCCCCCGGCC 
TCGCCCACAC ACAGCCCATC CTTCCCCATC CCTGATCGAT GTGAGGGCAA TTTTGACGCC 
ATCGCCAACA TCCGAGGGGA AACTTTCTTC TTCAAAGGCC CCTGGTTCTG GCGCCTCCAG 
CCCTCCGGAC AGCTGGTGTC CCCGCGACCC GCACGGCTGC ACCGCTTCTG GGAGGGGCTG 
CCCGCCCAGG TGAGGGTGGT GCAGGCCGCC TATGCTCGGC ACCGAGACGG CCGAATCCTC 
CTCTTTAGCG GGCCCCAGTT CTGGGTGTTC CAGGACCGGC AGCTGGAGGG CGGGGCGCGG 
CCGCTCACGG AGCTGGGGCT GCCCCCGGGA GAGGAGGTGG ACGCCGTGTT CTCGTGGCCA 
CAGAACGGGA AGACCTACCT GGTCCGCGGC CGGCAGTACT GGCGCTACGA CGAGGCGGCG 
GCGCGCCCGG ACCCCGGCTA CCCTCGCGAC CTGAGCCTCT GGGAAGGCGC GCCCCCCTCC 
CCTGACGATG TCACCGTCAG CAACGCAGGT GACACCTACT TCTTCAAGGG CGCCCACTAC 
TGGCGCTTCC CCAAGAACAG CATCAAGACC GAGCCGGACG CCCCCCAGCC CATGGGGCCC 
AACTGGCTGG ACTGCCCCGC CCCGAGCTCT GGTCCCCGCG CCCCCAGGCC CCCCAAAGCG 
ACCCCCGTGT CCGAAACCTG CGATTGTCAG TGCGAGCTCA ACCAGGCCGC AGGACGTTGG 
CCTGCTCCCA TCCCGCTGCT CCTCTTGCCC CTGCTGGTGG GGGGTGTAGC CTCCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMP25 ( XM_017023561.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879050.1 Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu86216
Clone ID Related Accession (Same CDS sequence)	XM_017023561.1
Accession Version	XM_017023561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	matrix metalloproteinase-25 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTGC GGCTCCGGCT TCTGGCGCTG CTGCTTCTGC TGCTGGCACC GCCCGCGCGC 
GCCCCGAAGC CCTCGGCGCA GGACGTGAGC CTGGGCGTGG ACTGGCTGAC TCGCTATGGT 
TACCTGCCGC CACCCCACCC TGCCCAGGCC CAGCTGCAGA GCCCTGAGAA GTTGCGCGAT 
GCCATCAAAG TCATGCAGAG GTTCGCGGGG CTGCCGGAGA CCGGCCGCAT GGACCCAGGG 
ACAGTGGCCA CCATGCGTAA GCCCCGCTGC TCCCTGCCTG ACGTGCTGGG GGTGGCGGGG 
CTGGTCAGGC GGCGTCGCCG GTACGCTCTG AGCGGCAGCG TGTGGAAGAA GCGAACCCTG 
ACATGGAGGG TACGTTCCTT CCCCCAGAGC TCCCAGCTGA GCCAGGAGAC CGTGCGGGTC 
CTCATGAGCT ATGCCCTGAT GGCCTGGGGC ATGGAGTCAG GCCTCACATT TCATGAGGTG 
GATTCCCCCC AGGGCCAGGA GCCCGACATC CTCATCGACT TTGCCCGCGC CTTCCACCAG 
GACAGCTACC CCTTCGACGG GTTGGGGGGC ACCCTAGCCC ATGCCTTCTT CCCTGGGGAG 
CACCCCATCT CCGGGGACAC TCACTTTGAC GATGAGGAGA CCTGGACTTT TGGGTCAAAA 
GATGCATGGC GTGACAAGGG TGCAGGAAGG GGTGCAGGGC CCGGAAGCCC AGTATGGCCA 
AGGAGAGACA AGGCAGGGAA GAAGGCCTCT CAGCAGCTGG AGCAGGAGCT GGCAGGCGGC 
TCACCGGTTG ATGAGGAGCT GGGCTTCAGC CGGGGCTGGC GTGTGAATCC TCTGGGTCCT 
GGCAGTCCTG AGCGCCTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879050.1
CDS	956..1819
Translation	MRLRLRLLALLLLLLAPPARAPKPSAQDVSLGVDWLTRYGYLPPPHPAQAQLQSPEKLRDAIKVMQRFAGLPETGRMDPGTVATMRKPRCSLPDVLGVAGLVRRRRRYALSGSVWKKRTLTWRVRSFPQSSQLSQETVRVLMSYALMAWGMESGLTFHEVDSPQGQEPDILIDFARAFHQDSYPFDGLGGTLAHAFFPGEHPISGDTHFDDEETWTFGSKDAWRDKGAGRGAGPGSPVWPRRDKAGKKASQQLEQELAGGSPVDEELGFSRGWRVNPLGPGSPERLS

Target ORF information:

RefSeq Version	XM_017023561.1
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 25 (MMP25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023561.1

ORF Insert Sequence:

ATGCGGCTGC GGCTCCGGCT TCTGGCGCTG CTGCTTCTGC TGCTGGCACC GCCCGCGCGC 
GCCCCGAAGC CCTCGGCGCA GGACGTGAGC CTGGGCGTGG ACTGGCTGAC TCGCTATGGT 
TACCTGCCGC CACCCCACCC TGCCCAGGCC CAGCTGCAGA GCCCTGAGAA GTTGCGCGAT 
GCCATCAAAG TCATGCAGAG GTTCGCGGGG CTGCCGGAGA CCGGCCGCAT GGACCCAGGG 
ACAGTGGCCA CCATGCGTAA GCCCCGCTGC TCCCTGCCTG ACGTGCTGGG GGTGGCGGGG 
CTGGTCAGGC GGCGTCGCCG GTACGCTCTG AGCGGCAGCG TGTGGAAGAA GCGAACCCTG 
ACATGGAGGG TACGTTCCTT CCCCCAGAGC TCCCAGCTGA GCCAGGAGAC CGTGCGGGTC 
CTCATGAGCT ATGCCCTGAT GGCCTGGGGC ATGGAGTCAG GCCTCACATT TCATGAGGTG 
GATTCCCCCC AGGGCCAGGA GCCCGACATC CTCATCGACT TTGCCCGCGC CTTCCACCAG 
GACAGCTACC CCTTCGACGG GTTGGGGGGC ACCCTAGCCC ATGCCTTCTT CCCTGGGGAG 
CACCCCATCT CCGGGGACAC TCACTTTGAC GATGAGGAGA CCTGGACTTT TGGGTCAAAA 
GATGCATGGC GTGACAAGGG TGCAGGAAGG GGTGCAGGGC CCGGAAGCCC AGTATGGCCA 
AGGAGAGACA AGGCAGGGAA GAAGGCCTCT CAGCAGCTGG AGCAGGAGCT GGCAGGCGGC 
TCACCGGTTG ATGAGGAGCT GGGCTTCAGC CGGGGCTGGC GTGTGAATCC TCTGGGTCCT 
GGCAGTCCTG AGCGCCTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of the matrix metalloproteases 2, 14, 24, and 25 and tissue inhibitor 3 as potential molecular markers in advanced human gastric cancer.
Disease markers2014285906(2014)
de la Pe?a S,Sampieri CL,Ochoa-Lara M,Le?n-C?rdoba K,Remes-Troche JM

Membrane-type 6 matrix metalloproteinase regulates the activation-induced downmodulation of CD16 in human primary NK cells.
Journal of immunology (Baltimore, Md. : 1950)191(4)1883-94(2013 Aug)
Peruzzi G,Femnou L,Gil-Krzewska A,Borrego F,Weck J,Krzewski K,Coligan JE

Biochemical characterization and N-terminomics analysis of leukolysin, the membrane-type 6 matrix metalloprotease (MMP25): chemokine and vimentin cleavages enhance cell migration and macrophage phagocytic activities.
The Journal of biological chemistry287(16)13382-95(2012 Apr)
Starr AE,Bellac CL,Dufour A,Goebeler V,Overall CM

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

Genetic variants in COL2A1, COL11A2, and IRF6 contribute risk to nonsyndromic cleft palate.
Birth defects research. Part A, Clinical and molecular teratology88(9)748-56(2010 Sep)
Nikopensius T,Jagom?gi T,Krjutskov K,Tammekivi V,Saag M,Prane I,Piekuse L,Akota I,Barkane B,Krumina A,Ambrozaityte L,Matuleviciene A,Kucinskiene ZA,Lace B,Kucinskas V,Metspalu A

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MMP25 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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