RHBDF1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017023556.1	XP_016879045.1	inactive rhomboid protein 1 isoform X1
XM_017023557.1	XP_016879046.1	inactive rhomboid protein 1 isoform X2
NM_022450.4	NP_071895.3	inactive rhomboid protein 1
XM_005255494.1	XP_005255551.1	inactive rhomboid protein 1 isoform X3
XM_017023558.1	XP_016879047.1	inactive rhomboid protein 1 isoform X4
XM_005255498.2	XP_005255555.1	inactive rhomboid protein 1 isoform X5
NM_022450.5	NP_071895.3	inactive rhomboid protein 1
NM_022450.3	NP_071895.3	inactive rhomboid protein 1
XM_006720921.1	XP_006720984.1	inactive rhomboid protein 1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu48918	XM_005255498.2 Latest version!	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu03868	XM_005255494.1 Latest version!	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu48916	XM_017023556.1 Latest version!	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu48916	XM_006720921.1 Latest version!	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu03868	NM_022450.5 Latest version!	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu86213	XM_017023557.1 Latest version!	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu86214	XM_017023558.1 Latest version!	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu03868	NM_022450.4	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu03868	NM_022450.3	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

RHBDF1 ( NM_022450.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071895.3 Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.

Price & Availability↑

CloneID	OHu03868
Clone ID Related Accession (Same CDS sequence)	NM_022450.4 , XM_005255494.1 , NM_022450.5 , NM_022450.3
Accession Version	NM_022450.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	inactive rhomboid protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK291177.1, BM830007.1 and BC014425.1. On Nov 23, 2018 this sequence version replaced NM_022450.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014425.1, AK026010.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC 
CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT 
TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC 
ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA 
CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT 
CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC 
CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA 
GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC 
TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA 
TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT 
TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC 
CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG 
GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC 
CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG 
CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC 
AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC 
GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA 
GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG 
GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG 
ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC 
CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC 
TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC 
ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC 
AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC 
GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC 
GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC 
ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC 
TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG 
CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC 
TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG 
CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT 
GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT 
GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC 
CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC 
TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC 
TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA 
CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC 
CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC 
ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071895.3
CDS	149..2716
Translation	MSEARRDSTSSLQRKKPPWLKLDIPSAVPLTAEEPSFLQPLRRQAFLRSVSMPAETAHISSPHHELRRPVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVLRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTAEGLSAPHTPVTPGAASLCSFSSSRSGFHRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRAQRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRSAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPIHPSAPELAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKNSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSICFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYVYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	NM_022450.4
Organism	Homo sapiens(human)
Definition	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022450.4

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC 
CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT 
TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC 
ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA 
CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT 
CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC 
CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA 
GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC 
TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA 
TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT 
TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC 
CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG 
GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC 
CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG 
CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC 
AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC 
GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA 
GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG 
GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG 
ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC 
CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC 
TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC 
ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC 
AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC 
GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC 
GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC 
ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC 
TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG 
CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC 
TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG 
CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT 
GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT 
GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC 
CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC 
TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC 
TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA 
CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC 
CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC 
ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_005255498.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255555.1 Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu48918
Clone ID Related Accession (Same CDS sequence)	XM_005255498.2
Accession Version	XM_005255498.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive rhomboid protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005255498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCCT TGGAGCGAGG CTGGCGGAAG CAGAAGGAGG GCGCCGCAGC CCCGCAGCCC 
AAGGTGCGGC TCCGACAGGA GGTGGTGAGC ACCGCGGGGC CGCGACGGGG CCAGCGTATC 
GCGGTGCCGG TGCGCAAGCT CTTCGCCCGG GAGAAGCGGC CGTATGGGCT GGGCATGGTG 
GGACGGCTCA CCAACCGCAC CTACCGCAAG CGCATCGACA GCTTCGTCAA GCGCCAGATC 
GAGGACATGG ACGACCACAG GCCCTTCTTC ACCTACTGGC TTACCTTCGT GCACTCGCTC 
GTCACCATCC TAGCCGTGTG CATCTATGGC ATCGCGCCCG TGGGCTTCTC GCAGCATGAG 
ACGGTGGACT CGGTGCTGCG GAACCGCGGG GTCTACGAGA ACGTCAAGTA CGTGCAGCAG 
GAGAACTTCT GGATCGGGCC CAGCTCGGAG GCCCTCATCC ACCTGGGCGC CAAGTTTTCG 
CCCTGCATGC GCCAGGACCC GCAGGTGCAC AGCTTCATTC GCTCGGCGCG CGAGCGCGAG 
AAGCACTCCG CCTGCTGCGT GCGCAACGAC AGGTCGGGCT GCGTGCAGAC CTCGGAGGAG 
GAGTGCTCGT CCACGCTGGC AGTGTGGGTG AAGTGGCCCA TCCATCCCAG CGCCCCAGAG 
CTTGCGGGCC ACAAGAGACA GTTTGGCTCT GTCTGCCACC AGGATCCCAG GGTGTGTGAT 
GAGCCCTCCT CCGAAGACCC TCATGAGTGG CCAGAAGACA TCACCAAGTG GCCGATCTGC 
ACCAAAAACA GCGCTGGGAA CCACACCAAC CATCCCCACA TGGACTGTGT CATCACAGGA 
CGGCCCTGCT GCATTGGCAC CAAGGGCAGG TGTGAGATCA CCTCCCGGGA GTACTGTGAC 
TTCATGAGGG GCTACTTCCA TGAGGAGGCC ACGCTCTGCT CTCAGGTGCA CTGCATGGAT 
GATGTGTGTG GGCTCCTGCC TTTTCTCAAC CCCGAGGTGC CTGACCAGTT CTACCGCCTG 
TGGCTATCCC TCTTCCTGCA CGCCGGGATC TTGCACTGCC TGGTGTCCAT CTGCTTCCAG 
ATGACTGTCC TGCGGGACCT GGAGAAGCTG GCAGGCTGGC ACCGCATAGC CATCATCTAC 
CTGCTGAGTG GTGTCACCGG CAACCTGGCC AGTGCCATCT TCCTGCCATA CCGAGCAGAG 
GTGGGTCCTG CTGGCTCCCA GTTCGGCATC CTGGCCTGCC TCTTCGTGGA GCTCTTCCAG 
AGCTGGCAGA TCCTGGCGCG GCCCTGGCGT GCCTTCTTCA AGCTGCTGGC TGTGGTGCTC 
TTCCTCTTCA CCTTTGGGCT GCTGCCGTGG ATTGACAACT TTGCCCACAT CTCAGGGTTC 
ATCAGTGGCC TCTTCCTCTC CTTCGCCTTC TTGCCCTACA TCAGCTTTGG CAAGTTCGAC 
CTGTACCGGA AACGCTGCCA GATCATCATC TTTCAGGTGG TCTTCCTGGG CCTCCTGGCT 
GGCCTGGTGG TCCTCTTCTA CGTCTATCCT GTCCGCTGTG AGTGGTGTGA GTTCCTCACC 
TGCATCCCCT TCACTGACAA GTTCTGTGAG AAGTACGAAC TGGACGCTCA GCTCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255555.1
CDS	947..2566
Translation	MLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRSAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPIHPSAPELAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKNSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSICFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYVYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_005255498.2
Organism	Homo sapiens(human)
Definition	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255498.2

ORF Insert Sequence:

ATGCTGCCCT TGGAGCGAGG CTGGCGGAAG CAGAAGGAGG GCGCCGCAGC CCCGCAGCCC 
AAGGTGCGGC TCCGACAGGA GGTGGTGAGC ACCGCGGGGC CGCGACGGGG CCAGCGTATC 
GCGGTGCCGG TGCGCAAGCT CTTCGCCCGG GAGAAGCGGC CGTATGGGCT GGGCATGGTG 
GGACGGCTCA CCAACCGCAC CTACCGCAAG CGCATCGACA GCTTCGTCAA GCGCCAGATC 
GAGGACATGG ACGACCACAG GCCCTTCTTC ACCTACTGGC TTACCTTCGT GCACTCGCTC 
GTCACCATCC TAGCCGTGTG CATCTATGGC ATCGCGCCCG TGGGCTTCTC GCAGCATGAG 
ACGGTGGACT CGGTGCTGCG GAACCGCGGG GTCTACGAGA ACGTCAAGTA CGTGCAGCAG 
GAGAACTTCT GGATCGGGCC CAGCTCGGAG GCCCTCATCC ACCTGGGCGC CAAGTTTTCG 
CCCTGCATGC GCCAGGACCC GCAGGTGCAC AGCTTCATTC GCTCGGCGCG CGAGCGCGAG 
AAGCACTCCG CCTGCTGCGT GCGCAACGAC AGGTCGGGCT GCGTGCAGAC CTCGGAGGAG 
GAGTGCTCGT CCACGCTGGC AGTGTGGGTG AAGTGGCCCA TCCATCCCAG CGCCCCAGAG 
CTTGCGGGCC ACAAGAGACA GTTTGGCTCT GTCTGCCACC AGGATCCCAG GGTGTGTGAT 
GAGCCCTCCT CCGAAGACCC TCATGAGTGG CCAGAAGACA TCACCAAGTG GCCGATCTGC 
ACCAAAAACA GCGCTGGGAA CCACACCAAC CATCCCCACA TGGACTGTGT CATCACAGGA 
CGGCCCTGCT GCATTGGCAC CAAGGGCAGG TGTGAGATCA CCTCCCGGGA GTACTGTGAC 
TTCATGAGGG GCTACTTCCA TGAGGAGGCC ACGCTCTGCT CTCAGGTGCA CTGCATGGAT 
GATGTGTGTG GGCTCCTGCC TTTTCTCAAC CCCGAGGTGC CTGACCAGTT CTACCGCCTG 
TGGCTATCCC TCTTCCTGCA CGCCGGGATC TTGCACTGCC TGGTGTCCAT CTGCTTCCAG 
ATGACTGTCC TGCGGGACCT GGAGAAGCTG GCAGGCTGGC ACCGCATAGC CATCATCTAC 
CTGCTGAGTG GTGTCACCGG CAACCTGGCC AGTGCCATCT TCCTGCCATA CCGAGCAGAG 
GTGGGTCCTG CTGGCTCCCA GTTCGGCATC CTGGCCTGCC TCTTCGTGGA GCTCTTCCAG 
AGCTGGCAGA TCCTGGCGCG GCCCTGGCGT GCCTTCTTCA AGCTGCTGGC TGTGGTGCTC 
TTCCTCTTCA CCTTTGGGCT GCTGCCGTGG ATTGACAACT TTGCCCACAT CTCAGGGTTC 
ATCAGTGGCC TCTTCCTCTC CTTCGCCTTC TTGCCCTACA TCAGCTTTGG CAAGTTCGAC 
CTGTACCGGA AACGCTGCCA GATCATCATC TTTCAGGTGG TCTTCCTGGG CCTCCTGGCT 
GGCCTGGTGG TCCTCTTCTA CGTCTATCCT GTCCGCTGTG AGTGGTGTGA GTTCCTCACC 
TGCATCCCCT TCACTGACAA GTTCTGTGAG AAGTACGAAC TGGACGCTCA GCTCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( NM_022450.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071895.3 Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.

Price & Availability↑

CloneID	OHu03868
Clone ID Related Accession (Same CDS sequence)	NM_022450.4 , XM_005255494.1 , NM_022450.5 , NM_022450.3
Accession Version	NM_022450.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	inactive rhomboid protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK291177.1, BM830007.1 and BC014425.1. On Jun 13, 2008 this sequence version replaced NM_022450.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014425.1, AK026010.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC 
CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT 
TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC 
ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA 
CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT 
CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC 
CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA 
GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC 
TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA 
TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT 
TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC 
CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG 
GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC 
CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG 
CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC 
AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC 
GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA 
GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG 
GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG 
ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC 
CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC 
TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC 
ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC 
AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC 
GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC 
GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC 
ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC 
TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG 
CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC 
TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG 
CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT 
GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT 
GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC 
CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC 
TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC 
TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA 
CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC 
CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC 
ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071895.3
CDS	149..2716
Translation	MSEARRDSTSSLQRKKPPWLKLDIPSAVPLTAEEPSFLQPLRRQAFLRSVSMPAETAHISSPHHELRRPVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVLRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTAEGLSAPHTPVTPGAASLCSFSSSRSGFHRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRAQRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRSAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPIHPSAPELAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKNSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSICFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYVYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	NM_022450.3
Organism	Homo sapiens(human)
Definition	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022450.3

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC 
CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT 
TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC 
ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA 
CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT 
CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC 
CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA 
GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC 
TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA 
TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT 
TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC 
CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG 
GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC 
CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG 
CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC 
AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC 
GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA 
GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG 
GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG 
ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC 
CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC 
TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC 
ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC 
AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC 
GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC 
GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC 
ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC 
TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG 
CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC 
TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG 
CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT 
GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT 
GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC 
CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC 
TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC 
TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA 
CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC 
CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC 
ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_005255494.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255551.1 Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA.

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CloneID	OHu03868
Clone ID Related Accession (Same CDS sequence)	NM_022450.4 , XM_005255494.1 , NM_022450.5 , NM_022450.3
Accession Version	XM_005255494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive rhomboid protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC 
CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT 
TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC 
ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA 
CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT 
CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC 
CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA 
GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC 
TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA 
TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT 
TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC 
CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG 
GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC 
CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG 
CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC 
AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC 
GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA 
GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG 
GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG 
ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC 
CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC 
TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC 
ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC 
AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC 
GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC 
GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC 
ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC 
TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG 
CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC 
TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG 
CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT 
GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT 
GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC 
CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC 
TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC 
TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA 
CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC 
CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC 
ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255551.1
CDS	128..2695
Translation	MSEARRDSTSSLQRKKPPWLKLDIPSAVPLTAEEPSFLQPLRRQAFLRSVSMPAETAHISSPHHELRRPVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVLRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTAEGLSAPHTPVTPGAASLCSFSSSRSGFHRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRAQRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRSAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPIHPSAPELAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKNSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSICFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYVYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_005255494.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255494.1

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC 
CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT 
TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC 
ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA 
CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT 
CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC 
CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA 
GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC 
TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA 
TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT 
TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC 
CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG 
GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC 
CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG 
CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC 
AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC 
GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA 
GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG 
GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG 
ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC 
CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC 
TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC 
ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC 
AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC 
GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC 
GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC 
ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC 
TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG 
CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC 
TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG 
CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT 
GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT 
GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC 
CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC 
TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC 
TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA 
CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC 
CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC 
ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_017023556.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879045.1 Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA.

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CloneID	OHu48916
Clone ID Related Accession (Same CDS sequence)	XM_017023556.1 , XM_006720921.1
Accession Version	XM_017023556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA 
GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT 
GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC 
CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC 
ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC 
ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG 
CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC 
GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC 
CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC 
CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA 
GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG 
GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG 
CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG 
CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG 
GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG 
CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG 
CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG 
GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG 
GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA 
CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG 
GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC 
ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG 
GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG 
AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC 
TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG 
CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG 
TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT 
GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG 
CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GATCTGCACC 
AAAAACAGCG CTGGGAACCA CACCAACCAT CCCCACATGG ACTGTGTCAT CACAGGACGG 
CCCTGCTGCA TTGGCACCAA GGGCAGGTGT GAGATCACCT CCCGGGAGTA CTGTGACTTC 
ATGAGGGGCT ACTTCCATGA GGAGGCCACG CTCTGCTCTC AGGTGCACTG CATGGATGAT 
GTGTGTGGGC TCCTGCCTTT TCTCAACCCC GAGGTGCCTG ACCAGTTCTA CCGCCTGTGG 
CTATCCCTCT TCCTGCACGC CGGGATCTTG CACTGCCTGG TGTCCATCTG CTTCCAGATG 
ACTGTCCTGC GGGACCTGGA GAAGCTGGCA GGCTGGCACC GCATAGCCAT CATCTACCTG 
CTGAGTGGTG TCACCGGCAA CCTGGCCAGT GCCATCTTCC TGCCATACCG AGCAGAGGTG 
GGTCCTGCTG GCTCCCAGTT CGGCATCCTG GCCTGCCTCT TCGTGGAGCT CTTCCAGAGC 
TGGCAGATCC TGGCGCGGCC CTGGCGTGCC TTCTTCAAGC TGCTGGCTGT GGTGCTCTTC 
CTCTTCACCT TTGGGCTGCT GCCGTGGATT GACAACTTTG CCCACATCTC AGGGTTCATC 
AGTGGCCTCT TCCTCTCCTT CGCCTTCTTG CCCTACATCA GCTTTGGCAA GTTCGACCTG 
TACCGGAAAC GCTGCCAGAT CATCATCTTT CAGGTGGTCT TCCTGGGCCT CCTGGCTGGC 
CTGGTGGTCC TCTTCTACGT CTATCCTGTC CGCTGTGAGT GGTGTGAGTT CCTCACCTGC 
ATCCCCTTCA CTGACAAGTT CTGTGAGAAG TACGAACTGG ACGCTCAGCT CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879045.1
CDS	114..2750
Translation	MSEARRDSTSSLQRKKPPWLKLDIPSAVPLTAEEPSFLQVGPGLQGLWVPPVQYPHHDPVAQPLRRQAFLRSVSMPAETAHISSPHHELRRPVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVLRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTAEGLSAPHTPVTPGAASLCSFSSSRSGFHRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRAQRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRSAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPIHPSAPELAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKNSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSICFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYVYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_017023556.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023556.1

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA 
GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT 
GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC 
CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC 
ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC 
ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG 
CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC 
GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC 
CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC 
CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA 
GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG 
GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG 
CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG 
CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG 
GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG 
CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG 
CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG 
GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG 
GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA 
CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG 
GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC 
ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG 
GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG 
AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC 
TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG 
CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG 
TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT 
GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG 
CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GATCTGCACC 
AAAAACAGCG CTGGGAACCA CACCAACCAT CCCCACATGG ACTGTGTCAT CACAGGACGG 
CCCTGCTGCA TTGGCACCAA GGGCAGGTGT GAGATCACCT CCCGGGAGTA CTGTGACTTC 
ATGAGGGGCT ACTTCCATGA GGAGGCCACG CTCTGCTCTC AGGTGCACTG CATGGATGAT 
GTGTGTGGGC TCCTGCCTTT TCTCAACCCC GAGGTGCCTG ACCAGTTCTA CCGCCTGTGG 
CTATCCCTCT TCCTGCACGC CGGGATCTTG CACTGCCTGG TGTCCATCTG CTTCCAGATG 
ACTGTCCTGC GGGACCTGGA GAAGCTGGCA GGCTGGCACC GCATAGCCAT CATCTACCTG 
CTGAGTGGTG TCACCGGCAA CCTGGCCAGT GCCATCTTCC TGCCATACCG AGCAGAGGTG 
GGTCCTGCTG GCTCCCAGTT CGGCATCCTG GCCTGCCTCT TCGTGGAGCT CTTCCAGAGC 
TGGCAGATCC TGGCGCGGCC CTGGCGTGCC TTCTTCAAGC TGCTGGCTGT GGTGCTCTTC 
CTCTTCACCT TTGGGCTGCT GCCGTGGATT GACAACTTTG CCCACATCTC AGGGTTCATC 
AGTGGCCTCT TCCTCTCCTT CGCCTTCTTG CCCTACATCA GCTTTGGCAA GTTCGACCTG 
TACCGGAAAC GCTGCCAGAT CATCATCTTT CAGGTGGTCT TCCTGGGCCT CCTGGCTGGC 
CTGGTGGTCC TCTTCTACGT CTATCCTGTC CGCTGTGAGT GGTGTGAGTT CCTCACCTGC 
ATCCCCTTCA CTGACAAGTT CTGTGAGAAG TACGAACTGG ACGCTCAGCT CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_006720921.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720984.1 Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA.

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CloneID	OHu48916
Clone ID Related Accession (Same CDS sequence)	XM_017023556.1 , XM_006720921.1
Accession Version	XM_006720921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA 
GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT 
GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC 
CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC 
ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC 
ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG 
CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC 
GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC 
CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC 
CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA 
GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG 
GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG 
CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG 
CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG 
GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG 
CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG 
CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG 
GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG 
GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA 
CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG 
GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC 
ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG 
GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG 
AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC 
TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG 
CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG 
TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT 
GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG 
CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GATCTGCACC 
AAAAACAGCG CTGGGAACCA CACCAACCAT CCCCACATGG ACTGTGTCAT CACAGGACGG 
CCCTGCTGCA TTGGCACCAA GGGCAGGTGT GAGATCACCT CCCGGGAGTA CTGTGACTTC 
ATGAGGGGCT ACTTCCATGA GGAGGCCACG CTCTGCTCTC AGGTGCACTG CATGGATGAT 
GTGTGTGGGC TCCTGCCTTT TCTCAACCCC GAGGTGCCTG ACCAGTTCTA CCGCCTGTGG 
CTATCCCTCT TCCTGCACGC CGGGATCTTG CACTGCCTGG TGTCCATCTG CTTCCAGATG 
ACTGTCCTGC GGGACCTGGA GAAGCTGGCA GGCTGGCACC GCATAGCCAT CATCTACCTG 
CTGAGTGGTG TCACCGGCAA CCTGGCCAGT GCCATCTTCC TGCCATACCG AGCAGAGGTG 
GGTCCTGCTG GCTCCCAGTT CGGCATCCTG GCCTGCCTCT TCGTGGAGCT CTTCCAGAGC 
TGGCAGATCC TGGCGCGGCC CTGGCGTGCC TTCTTCAAGC TGCTGGCTGT GGTGCTCTTC 
CTCTTCACCT TTGGGCTGCT GCCGTGGATT GACAACTTTG CCCACATCTC AGGGTTCATC 
AGTGGCCTCT TCCTCTCCTT CGCCTTCTTG CCCTACATCA GCTTTGGCAA GTTCGACCTG 
TACCGGAAAC GCTGCCAGAT CATCATCTTT CAGGTGGTCT TCCTGGGCCT CCTGGCTGGC 
CTGGTGGTCC TCTTCTACGT CTATCCTGTC CGCTGTGAGT GGTGTGAGTT CCTCACCTGC 
ATCCCCTTCA CTGACAAGTT CTGTGAGAAG TACGAACTGG ACGCTCAGCT CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720984.1
CDS	142..2778
Translation	MSEARRDSTSSLQRKKPPWLKLDIPSAVPLTAEEPSFLQVGPGLQGLWVPPVQYPHHDPVAQPLRRQAFLRSVSMPAETAHISSPHHELRRPVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVLRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTAEGLSAPHTPVTPGAASLCSFSSSRSGFHRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRAQRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRSAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPIHPSAPELAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKNSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSICFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYVYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_006720921.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720921.1

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA 
GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT 
GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC 
CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC 
ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC 
ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG 
CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC 
GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC 
CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC 
CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA 
GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG 
GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG 
CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG 
CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG 
GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG 
CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG 
CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG 
GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG 
GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA 
CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG 
GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC 
ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG 
GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG 
AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC 
TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG 
CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG 
TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT 
GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG 
CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GATCTGCACC 
AAAAACAGCG CTGGGAACCA CACCAACCAT CCCCACATGG ACTGTGTCAT CACAGGACGG 
CCCTGCTGCA TTGGCACCAA GGGCAGGTGT GAGATCACCT CCCGGGAGTA CTGTGACTTC 
ATGAGGGGCT ACTTCCATGA GGAGGCCACG CTCTGCTCTC AGGTGCACTG CATGGATGAT 
GTGTGTGGGC TCCTGCCTTT TCTCAACCCC GAGGTGCCTG ACCAGTTCTA CCGCCTGTGG 
CTATCCCTCT TCCTGCACGC CGGGATCTTG CACTGCCTGG TGTCCATCTG CTTCCAGATG 
ACTGTCCTGC GGGACCTGGA GAAGCTGGCA GGCTGGCACC GCATAGCCAT CATCTACCTG 
CTGAGTGGTG TCACCGGCAA CCTGGCCAGT GCCATCTTCC TGCCATACCG AGCAGAGGTG 
GGTCCTGCTG GCTCCCAGTT CGGCATCCTG GCCTGCCTCT TCGTGGAGCT CTTCCAGAGC 
TGGCAGATCC TGGCGCGGCC CTGGCGTGCC TTCTTCAAGC TGCTGGCTGT GGTGCTCTTC 
CTCTTCACCT TTGGGCTGCT GCCGTGGATT GACAACTTTG CCCACATCTC AGGGTTCATC 
AGTGGCCTCT TCCTCTCCTT CGCCTTCTTG CCCTACATCA GCTTTGGCAA GTTCGACCTG 
TACCGGAAAC GCTGCCAGAT CATCATCTTT CAGGTGGTCT TCCTGGGCCT CCTGGCTGGC 
CTGGTGGTCC TCTTCTACGT CTATCCTGTC CGCTGTGAGT GGTGTGAGTT CCTCACCTGC 
ATCCCCTTCA CTGACAAGTT CTGTGAGAAG TACGAACTGG ACGCTCAGCT CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( NM_022450.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071895.3 Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.

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CloneID	OHu03868
Clone ID Related Accession (Same CDS sequence)	NM_022450.4 , XM_005255494.1 , NM_022450.5 , NM_022450.3
Accession Version	NM_022450.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	inactive rhomboid protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from Z69720.1, Z69719.1, KF511226.1 and KF511222.1. On Aug 31, 2019 this sequence version replaced NM_022450.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014425.1, AK291177.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262316.10/ ENSP00000262316.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC 
CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT 
TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC 
ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA 
CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT 
CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC 
CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA 
GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC 
TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA 
TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT 
TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC 
CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG 
GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC 
CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG 
CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC 
AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC 
GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA 
GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG 
GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG 
ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC 
CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC 
TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC 
ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC 
AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC 
GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC 
GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC 
ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC 
TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG 
CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC 
TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG 
CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT 
GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT 
GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC 
CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC 
TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC 
TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA 
CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC 
CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC 
ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071895.3
CDS	144..2711
Translation	MSEARRDSTSSLQRKKPPWLKLDIPSAVPLTAEEPSFLQPLRRQAFLRSVSMPAETAHISSPHHELRRPVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVLRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTAEGLSAPHTPVTPGAASLCSFSSSRSGFHRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRAQRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRSAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPIHPSAPELAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKNSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSICFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYVYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	NM_022450.5
Organism	Homo sapiens(human)
Definition	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022450.5

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC 
CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT 
TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC 
ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA 
CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT 
CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC 
CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA 
GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC 
TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA 
TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT 
TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC 
CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG 
GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC 
CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG 
CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC 
AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC 
GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA 
GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG 
GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG 
ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC 
CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC 
TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC 
ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC 
AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC 
GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC 
GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC 
ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC 
TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG 
CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC 
TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG 
CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT 
GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT 
GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC 
CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC 
TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC 
TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA 
CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC 
CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC 
ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_017023557.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879046.1 Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu86213
Clone ID Related Accession (Same CDS sequence)	XM_017023557.1
Accession Version	XM_017023557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive rhomboid protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA 
GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT 
GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC 
CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC 
ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC 
ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG 
CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC 
GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC 
CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC 
CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA 
GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG 
GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG 
CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG 
CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG 
GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG 
CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG 
CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG 
GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG 
GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA 
CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG 
GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC 
ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG 
GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG 
AACTTCTGGA TCGGGCCCAG CTCGTCCACG CTGGCAGTGT GGGTGAAGTG GCCCATCCAT 
CCCAGCGCCC CAGAGCTTGC GGGCCACAAG AGACAGTTTG GCTCTGTCTG CCACCAGGAT 
CCCAGGGTGT GTGATGAGCC CTCCTCCGAA GACCCTCATG AGTGGCCAGA AGACATCACC 
AAGTGGCCGA TCTGCACCAA AAACAGCGCT GGGAACCACA CCAACCATCC CCACATGGAC 
TGTGTCATCA CAGGACGGCC CTGCTGCATT GGCACCAAGG GCAGGTGTGA GATCACCTCC 
CGGGAGTACT GTGACTTCAT GAGGGGCTAC TTCCATGAGG AGGCCACGCT CTGCTCTCAG 
GTGCACTGCA TGGATGATGT GTGTGGGCTC CTGCCTTTTC TCAACCCCGA GGTGCCTGAC 
CAGTTCTACC GCCTGTGGCT ATCCCTCTTC CTGCACGCCG GGATCTTGCA CTGCCTGGTG 
TCCATCTGCT TCCAGATGAC TGTCCTGCGG GACCTGGAGA AGCTGGCAGG CTGGCACCGC 
ATAGCCATCA TCTACCTGCT GAGTGGTGTC ACCGGCAACC TGGCCAGTGC CATCTTCCTG 
CCATACCGAG CAGAGGTGGG TCCTGCTGGC TCCCAGTTCG GCATCCTGGC CTGCCTCTTC 
GTGGAGCTCT TCCAGAGCTG GCAGATCCTG GCGCGGCCCT GGCGTGCCTT CTTCAAGCTG 
CTGGCTGTGG TGCTCTTCCT CTTCACCTTT GGGCTGCTGC CGTGGATTGA CAACTTTGCC 
CACATCTCAG GGTTCATCAG TGGCCTCTTC CTCTCCTTCG CCTTCTTGCC CTACATCAGC 
TTTGGCAAGT TCGACCTGTA CCGGAAACGC TGCCAGATCA TCATCTTTCA GGTGGTCTTC 
CTGGGCCTCC TGGCTGGCCT GGTGGTCCTC TTCTACGTCT ATCCTGTCCG CTGTGAGTGG 
TGTGAGTTCC TCACCTGCAT CCCCTTCACT GACAAGTTCT GTGAGAAGTA CGAACTGGAC 
GCTCAGCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879046.1
CDS	25..2499
Translation	MSEARRDSTSSLQRKKPPWLKLDIPSAVPLTAEEPSFLQVGPGLQGLWVPPVQYPHHDPVAQPLRRQAFLRSVSMPAETAHISSPHHELRRPVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVLRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTAEGLSAPHTPVTPGAASLCSFSSSRSGFHRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRAQRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSSTLAVWVKWPIHPSAPELAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKNSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSICFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYVYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_017023557.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023557.1

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA 
GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT 
GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC 
CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC 
ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC 
ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG 
CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC 
GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC 
CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC 
CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA 
GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG 
GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG 
CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG 
CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG 
GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG 
CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG 
CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG 
GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG 
GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA 
CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG 
GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC 
ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG 
GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG 
AACTTCTGGA TCGGGCCCAG CTCGTCCACG CTGGCAGTGT GGGTGAAGTG GCCCATCCAT 
CCCAGCGCCC CAGAGCTTGC GGGCCACAAG AGACAGTTTG GCTCTGTCTG CCACCAGGAT 
CCCAGGGTGT GTGATGAGCC CTCCTCCGAA GACCCTCATG AGTGGCCAGA AGACATCACC 
AAGTGGCCGA TCTGCACCAA AAACAGCGCT GGGAACCACA CCAACCATCC CCACATGGAC 
TGTGTCATCA CAGGACGGCC CTGCTGCATT GGCACCAAGG GCAGGTGTGA GATCACCTCC 
CGGGAGTACT GTGACTTCAT GAGGGGCTAC TTCCATGAGG AGGCCACGCT CTGCTCTCAG 
GTGCACTGCA TGGATGATGT GTGTGGGCTC CTGCCTTTTC TCAACCCCGA GGTGCCTGAC 
CAGTTCTACC GCCTGTGGCT ATCCCTCTTC CTGCACGCCG GGATCTTGCA CTGCCTGGTG 
TCCATCTGCT TCCAGATGAC TGTCCTGCGG GACCTGGAGA AGCTGGCAGG CTGGCACCGC 
ATAGCCATCA TCTACCTGCT GAGTGGTGTC ACCGGCAACC TGGCCAGTGC CATCTTCCTG 
CCATACCGAG CAGAGGTGGG TCCTGCTGGC TCCCAGTTCG GCATCCTGGC CTGCCTCTTC 
GTGGAGCTCT TCCAGAGCTG GCAGATCCTG GCGCGGCCCT GGCGTGCCTT CTTCAAGCTG 
CTGGCTGTGG TGCTCTTCCT CTTCACCTTT GGGCTGCTGC CGTGGATTGA CAACTTTGCC 
CACATCTCAG GGTTCATCAG TGGCCTCTTC CTCTCCTTCG CCTTCTTGCC CTACATCAGC 
TTTGGCAAGT TCGACCTGTA CCGGAAACGC TGCCAGATCA TCATCTTTCA GGTGGTCTTC 
CTGGGCCTCC TGGCTGGCCT GGTGGTCCTC TTCTACGTCT ATCCTGTCCG CTGTGAGTGG 
TGTGAGTTCC TCACCTGCAT CCCCTTCACT GACAAGTTCT GTGAGAAGTA CGAACTGGAC 
GCTCAGCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_017023558.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879047.1 Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu86214
Clone ID Related Accession (Same CDS sequence)	XM_017023558.1
Accession Version	XM_017023558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive rhomboid protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA 
GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT 
GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC 
CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC 
ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC 
ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG 
CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC 
GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC 
CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC 
CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA 
GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG 
GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG 
CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG 
CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG 
GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG 
CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG 
CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG 
GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG 
GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA 
CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG 
GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC 
ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG 
GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG 
AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC 
TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG 
CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG 
TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT 
GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG 
CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GAGGCTGTGC 
ACCTGCCCTT TGTACAGCCT GTCCATCAAC CAGCGCCTGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879047.1
CDS	25..1872
Translation	MSEARRDSTSSLQRKKPPWLKLDIPSAVPLTAEEPSFLQVGPGLQGLWVPPVQYPHHDPVAQPLRRQAFLRSVSMPAETAHISSPHHELRRPVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVLRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTAEGLSAPHTPVTPGAASLCSFSSSRSGFHRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRAQRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRSAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPIHPSAPELAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPRLCTCPLYSLSINQRLPL

Target ORF information:

RefSeq Version	XM_017023558.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023558.1

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA 
GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT 
GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC 
CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC 
ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC 
ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG 
CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC 
GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC 
CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC 
CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA 
GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG 
GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG 
CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG 
CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG 
GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG 
CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG 
CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG 
GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG 
GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA 
CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG 
GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC 
ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG 
GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG 
AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC 
TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG 
CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG 
TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT 
GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG 
CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GAGGCTGTGC 
ACCTGCCCTT TGTACAGCCT GTCCATCAAC CAGCGCCTGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RHBDF1
Entrez Gene ID	64285
Full Name	rhomboid 5 homolog 1
Synonyms	C16orf8,Dist1,EGFR-RS,gene-89,gene-90,hDist1
General protein information	Preferred Names rhomboid 5 homolog 1 Names inactive rhomboid protein 1 epidermal growth factor receptor, related sequence epidermal growth factor receptor-related protein iRhom1 p100hRho rhomboid family 1 rhomboid family member 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Disorder MIM:	614403

Pan troglodytes (chimpanzee)	RHBDF1	XP_510701.3
Rattus norvegicus (Norway rat)	Rhbdf1	NP_001025205.1
Danio rerio (zebrafish)	rhbdf1	NP_001002228.1
Xenopus tropicalis (tropical clawed frog)	rhbdf1	XP_004917998.1
Homo sapiens (human)	RHBDF1	NP_071895.3
Gallus gallus (chicken)	RHBDF1	XP_004945406.1
Canis lupus familiaris (dog)	RHBDF1	XP_005621827.1
Bos taurus (cattle)	RHBDF1	NP_001098870.1
Mus musculus (house mouse)	Rhbdf1	NP_034247.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RHBDF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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