TFB2M cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TFB2M
Entrez Gene ID	64216
Full Name	transcription factor B2, mitochondrial
Synonyms	Hkp1,mtTFB2
General protein information	Preferred Names transcription factor B2, mitochondrial Names dimethyladenosine transferase 2, mitochondrial HCV NS5A-transactivated protein 5 S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2 h-mtTFB h-mtTFB2 hTFB2M hepatitis C virus NS5A-transactivated protein 5 mitochondrial 12S rRNA dimethylase 2 mitochondrial transcription factor B2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q44
Disorder MIM:	607055

mRNA and Protein(s)

mRNA	Protein	Name
XM_011544248.2	XP_011542550.1	dimethyladenosine transferase 2, mitochondrial isoform X1
NM_022366.3	NP_071761.1	dimethyladenosine transferase 2, mitochondrial
NM_022366.2	NP_071761.1	dimethyladenosine transferase 2, mitochondrial
XM_017002055.1	XP_016857544.1	dimethyladenosine transferase 2, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TFB2M	NP_071761.1
Pan troglodytes (chimpanzee)	TFB2M	XP_001139400.1
Canis lupus familiaris (dog)	TFB2M	XP_537224.2
Rattus norvegicus (Norway rat)	Tfb2m	NP_001008294.1
Danio rerio (zebrafish)	tfb2m	NP_001107089.1
Bos taurus (cattle)	TFB2M	NP_001033216.1
Mus musculus (house mouse)	Tfb2m	NP_032275.2
Gallus gallus (chicken)	TFB2M	XP_419535.2
Xenopus tropicalis (tropical clawed frog)	tfb2m	XP_004914681.1
	TFB2M	XP_001088795.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural Basis of Mitochondrial Transcription Initiation.
Hillen HS, Morozov YI, Sarfallah A, Temiakov D, Cramer P
Cell171(5)1072-1081.e10(2017 Nov)

Human Mitochondrial Transcription Factor B2 Is Required for Promoter Melting during Initiation of Transcription.
Posse V, Gustafsson CM
The Journal of biological chemistry292(7)2637-2645(2017 02)

Organization of the human mitochondrial transcription initiation complex.
Yakubovskaya E, Guja KE, Eng ET, Choi WS, Mejia E, Beglov D, Lukin M, Kozakov D, Garcia-Diaz M
Nucleic acids research42(6)4100-12(2014 Apr)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reports crystal structures of human mitochondrial transcription initiation complexes assembled on both light and heavy strand promoters. The structures reveal how transcription factors TFAM and TFB2M assist mitochondrial RNA polymerase to achieve promoter-dependent initiation.
Title: Structural Basis of Mitochondrial Transcription Initiation.

demonstrate that a complex composed of TFAM and POLRMT was readily formed at the promoter but alone was insufficient for promoter melting, which only occurred when TFB2M joined the complex
Title: Human Mitochondrial Transcription Factor B2 Is Required for Promoter Melting during Initiation of Transcription.

The results reveal the organization of TFAM, POLRMT and TFB2M around the light-strand promoter and represent the first structural characterization of the entire mitochondrial transcriptional initiation complex.
Title: Organization of the human mitochondrial transcription initiation complex.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

only two essential initiation factors, TFAM and TFB2M, and two promoters, LSP and HSP1, are required to drive transcription of the mitochondrial genome
Title: Human mitochondrial transcription revisited: only TFAM and TFB2M are required for transcription of the mitochondrial genes in vitro.

The following TFB2M gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TFB2M cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09059	NM_022366.3 Latest version!	Homo sapiens transcription factor B2, mitochondrial (TFB2M), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu71290	XM_011544248.2 Latest version!	Homo sapiens transcription factor B2, mitochondrial (TFB2M), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu90382	XM_017002055.1 Latest version!	Homo sapiens transcription factor B2, mitochondrial (TFB2M), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu09059	NM_022366.2	Homo sapiens transcription factor B2, mitochondrial (TFB2M), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TFB2M ( NM_022366.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071761.1 Homo sapiens transcription factor B2, mitochondrial (TFB2M), mRNA.

Price & Availability↑

CloneID	OHu09059
Clone ID Related Accession (Same CDS sequence)	NM_022366.3 , NM_022366.2
Accession Version	NM_022366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	dimethyladenosine transferase 2, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA038232.1, AK026835.1 and CK725134.1. On Nov 22, 2018 this sequence version replaced NM_022366.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1178097.1, SRR1660805.64358.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000366514.5/ ENSP00000355471.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGATCC CAGTGGTCGG GCTTCCTCGG CGGCTGAGGC TCTCCGCCTT GGCGGGCGCT 
GGTCGCTTTT GCATTTTAGG GTCTGAAGCG GCGACGCGAA AGCATTTGCC GGCGAGGAAC 
CACTGTGGGC TCTCTGACTC CTCTCCGCAG CTGTGGCCCG AACCGGATTT CAGGAATCCG 
CCAAGGAAGG CGTCTAAGGC CAGCTTAGAC TTTAAGCGTT ACGTAACCGA TCGGAGATTG 
GCTGAGACCC TGGCGCAAAT CTATTTGGGA AAACCAAGTA GACCTCCACA CCTACTGCTG 
GAGTGCAATC CAGGTCCTGG AATCCTGACT CAGGCATTAC TTGAAGCTGG TGCCAAAGTG 
GTTGCGCTCG AAAGTGACAA AACTTTTATT CCACATTTGG AGTCCTTAGG AAAAAATCTG 
GATGGAAAAC TACGAGTGAT TCACTGTGAC TTCTTTAAAC TAGATCCTAG AAGTGGTGGA 
GTAATAAAAC CACCTGCTAT GTCTTCTCGA GGGCTCTTTA AGAATTTGGG AATAGAAGCA 
GTTCCTTGGA CAGCAGACAT CCCTTTAAAA GTAGTTGGAA TGTTCCCAAG TAGAGGTGAG 
AAAAGGGCAC TTTGGAAACT CGCATATGAC TTGTATTCCT GTACTTCTAT ATATAAATTT 
GGACGAATAG AAGTAAATAT GTTTATTGGT GAAAAAGAAT TCCAGAAACT AATGGCAGAT 
CCCGGAAATC CAGACTTGTA TCATGTATTA AGTGTTATCT GGCAATTAGC TTGTGAGATT 
AAGGTTCTGC ACATGGAGCC TTGGTCATCA TTTGATATAT ACACCCGGAA AGGGCCGCTG 
GAAAACCCAA AGCGTAGGGA ATTATTAGAC CAATTACAAC AAAAGCTGTA TCTTATTCAA 
ATGATTCCTC GTCAAAATTT ATTTACCAAG AACTTAACAC CTATGAACTA TAATATATTT 
TTTCACTTGT TAAAGCACTG TTTTGGGAGG CGCAGCGCCA CTGTAATAGA CCACTTACGT 
TCATTGACTC CACTTGATGC GAGAGATATA TTGATGCAAA TAGGAAAACA GGAGGATGAG 
AAAGTAGTTA ACATGCACCC TCAAGACTTC AAAACACTTT TTGAAACTAT AGAGCGTTCC 
AAAGATTGTG CTTATAAATG GCTGTATGAT GAAACCCTGG AAGATAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071761.1
CDS	124..1314
Translation	MWIPVVGLPRRLRLSALAGAGRFCILGSEAATRKHLPARNHCGLSDSSPQLWPEPDFRNPPRKASKASLDFKRYVTDRRLAETLAQIYLGKPSRPPHLLLECNPGPGILTQALLEAGAKVVALESDKTFIPHLESLGKNLDGKLRVIHCDFFKLDPRSGGVIKPPAMSSRGLFKNLGIEAVPWTADIPLKVVGMFPSRGEKRALWKLAYDLYSCTSIYKFGRIEVNMFIGEKEFQKLMADPGNPDLYHVLSVIWQLACEIKVLHMEPWSSFDIYTRKGPLENPKRRELLDQLQQKLYLIQMIPRQNLFTKNLTPMNYNIFFHLLKHCFGRRSATVIDHLRSLTPLDARDILMQIGKQEDEKVVNMHPQDFKTLFETIERSKDCAYKWLYDETLEDR

Target ORF information:

RefSeq Version	NM_022366.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription factor B2, mitochondrial (TFB2M), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022366.3

ORF Insert Sequence:

ATGTGGATCC CAGTGGTCGG GCTTCCTCGG CGGCTGAGGC TCTCCGCCTT GGCGGGCGCT 
GGTCGCTTTT GCATTTTAGG GTCTGAAGCG GCGACGCGAA AGCATTTGCC GGCGAGGAAC 
CACTGTGGGC TCTCTGACTC CTCTCCGCAG CTGTGGCCCG AACCGGATTT CAGGAATCCG 
CCAAGGAAGG CGTCTAAGGC CAGCTTAGAC TTTAAGCGTT ACGTAACCGA TCGGAGATTG 
GCTGAGACCC TGGCGCAAAT CTATTTGGGA AAACCAAGTA GACCTCCACA CCTACTGCTG 
GAGTGCAATC CAGGTCCTGG AATCCTGACT CAGGCATTAC TTGAAGCTGG TGCCAAAGTG 
GTTGCGCTCG AAAGTGACAA AACTTTTATT CCACATTTGG AGTCCTTAGG AAAAAATCTG 
GATGGAAAAC TACGAGTGAT TCACTGTGAC TTCTTTAAAC TAGATCCTAG AAGTGGTGGA 
GTAATAAAAC CACCTGCTAT GTCTTCTCGA GGGCTCTTTA AGAATTTGGG AATAGAAGCA 
GTTCCTTGGA CAGCAGACAT CCCTTTAAAA GTAGTTGGAA TGTTCCCAAG TAGAGGTGAG 
AAAAGGGCAC TTTGGAAACT CGCATATGAC TTGTATTCCT GTACTTCTAT ATATAAATTT 
GGACGAATAG AAGTAAATAT GTTTATTGGT GAAAAAGAAT TCCAGAAACT AATGGCAGAT 
CCCGGAAATC CAGACTTGTA TCATGTATTA AGTGTTATCT GGCAATTAGC TTGTGAGATT 
AAGGTTCTGC ACATGGAGCC TTGGTCATCA TTTGATATAT ACACCCGGAA AGGGCCGCTG 
GAAAACCCAA AGCGTAGGGA ATTATTAGAC CAATTACAAC AAAAGCTGTA TCTTATTCAA 
ATGATTCCTC GTCAAAATTT ATTTACCAAG AACTTAACAC CTATGAACTA TAATATATTT 
TTTCACTTGT TAAAGCACTG TTTTGGGAGG CGCAGCGCCA CTGTAATAGA CCACTTACGT 
TCATTGACTC CACTTGATGC GAGAGATATA TTGATGCAAA TAGGAAAACA GGAGGATGAG 
AAAGTAGTTA ACATGCACCC TCAAGACTTC AAAACACTTT TTGAAACTAT AGAGCGTTCC 
AAAGATTGTG CTTATAAATG GCTGTATGAT GAAACCCTGG AAGATAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TFB2M ( NM_022366.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_071761.1 Homo sapiens transcription factor B2, mitochondrial (TFB2M), mRNA.

Price & Availability↑

CloneID	OHu09059
Clone ID Related Accession (Same CDS sequence)	NM_022366.3 , NM_022366.2
Accession Version	NM_022366.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	dimethyladenosine transferase 2, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA038232.1, AK026835.1 and CK725134.1. On Oct 31, 2008 this sequence version replaced NM_022366.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.458547.1, AK026835.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGATCC CAGTGGTCGG GCTTCCTCGG CGGCTGAGGC TCTCCGCCTT GGCGGGCGCT 
GGTCGCTTTT GCATTTTAGG GTCTGAAGCG GCGACGCGAA AGCATTTGCC GGCGAGGAAC 
CACTGTGGGC TCTCTGACTC CTCTCCGCAG CTGTGGCCCG AACCGGATTT CAGGAATCCG 
CCAAGGAAGG CGTCTAAGGC CAGCTTAGAC TTTAAGCGTT ACGTAACCGA TCGGAGATTG 
GCTGAGACCC TGGCGCAAAT CTATTTGGGA AAACCAAGTA GACCTCCACA CCTACTGCTG 
GAGTGCAATC CAGGTCCTGG AATCCTGACT CAGGCATTAC TTGAAGCTGG TGCCAAAGTG 
GTTGCGCTCG AAAGTGACAA AACTTTTATT CCACATTTGG AGTCCTTAGG AAAAAATCTG 
GATGGAAAAC TACGAGTGAT TCACTGTGAC TTCTTTAAAC TAGATCCTAG AAGTGGTGGA 
GTAATAAAAC CACCTGCTAT GTCTTCTCGA GGGCTCTTTA AGAATTTGGG AATAGAAGCA 
GTTCCTTGGA CAGCAGACAT CCCTTTAAAA GTAGTTGGAA TGTTCCCAAG TAGAGGTGAG 
AAAAGGGCAC TTTGGAAACT CGCATATGAC TTGTATTCCT GTACTTCTAT ATATAAATTT 
GGACGAATAG AAGTAAATAT GTTTATTGGT GAAAAAGAAT TCCAGAAACT AATGGCAGAT 
CCCGGAAATC CAGACTTGTA TCATGTATTA AGTGTTATCT GGCAATTAGC TTGTGAGATT 
AAGGTTCTGC ACATGGAGCC TTGGTCATCA TTTGATATAT ACACCCGGAA AGGGCCGCTG 
GAAAACCCAA AGCGTAGGGA ATTATTAGAC CAATTACAAC AAAAGCTGTA TCTTATTCAA 
ATGATTCCTC GTCAAAATTT ATTTACCAAG AACTTAACAC CTATGAACTA TAATATATTT 
TTTCACTTGT TAAAGCACTG TTTTGGGAGG CGCAGCGCCA CTGTAATAGA CCACTTACGT 
TCATTGACTC CACTTGATGC GAGAGATATA TTGATGCAAA TAGGAAAACA GGAGGATGAG 
AAAGTAGTTA ACATGCACCC TCAAGACTTC AAAACACTTT TTGAAACTAT AGAGCGTTCC 
AAAGATTGTG CTTATAAATG GCTGTATGAT GAAACCCTGG AAGATAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071761.1
CDS	126..1316
Translation	MWIPVVGLPRRLRLSALAGAGRFCILGSEAATRKHLPARNHCGLSDSSPQLWPEPDFRNPPRKASKASLDFKRYVTDRRLAETLAQIYLGKPSRPPHLLLECNPGPGILTQALLEAGAKVVALESDKTFIPHLESLGKNLDGKLRVIHCDFFKLDPRSGGVIKPPAMSSRGLFKNLGIEAVPWTADIPLKVVGMFPSRGEKRALWKLAYDLYSCTSIYKFGRIEVNMFIGEKEFQKLMADPGNPDLYHVLSVIWQLACEIKVLHMEPWSSFDIYTRKGPLENPKRRELLDQLQQKLYLIQMIPRQNLFTKNLTPMNYNIFFHLLKHCFGRRSATVIDHLRSLTPLDARDILMQIGKQEDEKVVNMHPQDFKTLFETIERSKDCAYKWLYDETLEDR

Target ORF information:

RefSeq Version	NM_022366.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription factor B2, mitochondrial (TFB2M), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022366.2

ORF Insert Sequence:

ATGTGGATCC CAGTGGTCGG GCTTCCTCGG CGGCTGAGGC TCTCCGCCTT GGCGGGCGCT 
GGTCGCTTTT GCATTTTAGG GTCTGAAGCG GCGACGCGAA AGCATTTGCC GGCGAGGAAC 
CACTGTGGGC TCTCTGACTC CTCTCCGCAG CTGTGGCCCG AACCGGATTT CAGGAATCCG 
CCAAGGAAGG CGTCTAAGGC CAGCTTAGAC TTTAAGCGTT ACGTAACCGA TCGGAGATTG 
GCTGAGACCC TGGCGCAAAT CTATTTGGGA AAACCAAGTA GACCTCCACA CCTACTGCTG 
GAGTGCAATC CAGGTCCTGG AATCCTGACT CAGGCATTAC TTGAAGCTGG TGCCAAAGTG 
GTTGCGCTCG AAAGTGACAA AACTTTTATT CCACATTTGG AGTCCTTAGG AAAAAATCTG 
GATGGAAAAC TACGAGTGAT TCACTGTGAC TTCTTTAAAC TAGATCCTAG AAGTGGTGGA 
GTAATAAAAC CACCTGCTAT GTCTTCTCGA GGGCTCTTTA AGAATTTGGG AATAGAAGCA 
GTTCCTTGGA CAGCAGACAT CCCTTTAAAA GTAGTTGGAA TGTTCCCAAG TAGAGGTGAG 
AAAAGGGCAC TTTGGAAACT CGCATATGAC TTGTATTCCT GTACTTCTAT ATATAAATTT 
GGACGAATAG AAGTAAATAT GTTTATTGGT GAAAAAGAAT TCCAGAAACT AATGGCAGAT 
CCCGGAAATC CAGACTTGTA TCATGTATTA AGTGTTATCT GGCAATTAGC TTGTGAGATT 
AAGGTTCTGC ACATGGAGCC TTGGTCATCA TTTGATATAT ACACCCGGAA AGGGCCGCTG 
GAAAACCCAA AGCGTAGGGA ATTATTAGAC CAATTACAAC AAAAGCTGTA TCTTATTCAA 
ATGATTCCTC GTCAAAATTT ATTTACCAAG AACTTAACAC CTATGAACTA TAATATATTT 
TTTCACTTGT TAAAGCACTG TTTTGGGAGG CGCAGCGCCA CTGTAATAGA CCACTTACGT 
TCATTGACTC CACTTGATGC GAGAGATATA TTGATGCAAA TAGGAAAACA GGAGGATGAG 
AAAGTAGTTA ACATGCACCC TCAAGACTTC AAAACACTTT TTGAAACTAT AGAGCGTTCC 
AAAGATTGTG CTTATAAATG GCTGTATGAT GAAACCCTGG AAGATAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TFB2M ( XM_011544248.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542550.1 Homo sapiens transcription factor B2, mitochondrial (TFB2M), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu71290
Clone ID Related Accession (Same CDS sequence)	XM_011544248.2
Accession Version	XM_011544248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dimethyladenosine transferase 2, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATCC CAGTGGTCGG GCTTCCTCGG CGGCTGAGGC TCTCCGCCTT GGCGGGCGCT 
GGTCGCTTTT GCATTTTAGG GTCTGAAGCG GCGACGCGAA AGCATTTGCC GGCGAGGAAC 
CACTGTGGGC TCTCTGACTC CTCTCCGCAG CTGTGGCCCG AACCGGATTT CAGGAATCCG 
CCAAGGAAGG CGTCTAAGGC CAGCTTAGAC TTTAAGCGTT ACGTAACCGA TCGGAGATTG 
GCTGAGACCC TGGCGCAAAT CTATTTGGGA AAACCAAGTA GACCTCCACA CCTACTGCTG 
GAGTGCAATC CAGGTCCTGG AATCCTGACT CAGGCATTAC TTGAAGCTGG TGCCAAAGTG 
GTTGCGCTCG AAAGTGACAA AACTTTTATT CCACATTTGG AGAAACTAAT GGCAGATCCC 
GGAAATCCAG ACTTGTATCA TGTATTAAGT GTTATCTGGC AATTAGCTTG TGAGATTAAG 
GTTCTGCACA TGGAGCCTTG GTCATCATTT GATATATACA CCCGGAAAGG GCCGCTGGAA 
AACCCAAAGC GTAGGGAATT ATTAGACCAA TTACAACAAA AGCTGTATCT TATTCAAATG 
ATTCCTCGTC AAAATTTATT TACCAAGAAC TTAACACCTA TGAACTATAA TATATTTTTT 
CACTTGTTAA AGCACTGTTT TGGGAGGCGC AGCGCCACTG TAATAGACCA CTTACGTTCA 
TTGACTCCAC TTGATGCGAG AGATATATTG ATGCAAATAG GAAAACAGGA GGATGAGAAA 
GTAGTTAACA TGCACCCTCA AGACTTCAAA ACACTTTTTG AAACTATAGA GCGTTCCAAA 
GATTGTGCTT ATAAATGGCT GTATGATGAA ACCCTGGAAG ATAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542550.1
CDS	124..1011
Translation	MWIPVVGLPRRLRLSALAGAGRFCILGSEAATRKHLPARNHCGLSDSSPQLWPEPDFRNPPRKASKASLDFKRYVTDRRLAETLAQIYLGKPSRPPHLLLECNPGPGILTQALLEAGAKVVALESDKTFIPHLEKLMADPGNPDLYHVLSVIWQLACEIKVLHMEPWSSFDIYTRKGPLENPKRRELLDQLQQKLYLIQMIPRQNLFTKNLTPMNYNIFFHLLKHCFGRRSATVIDHLRSLTPLDARDILMQIGKQEDEKVVNMHPQDFKTLFETIERSKDCAYKWLYDETLEDR

Target ORF information:

RefSeq Version	XM_011544248.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription factor B2, mitochondrial (TFB2M), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544248.2

ORF Insert Sequence:

ATGTGGATCC CAGTGGTCGG GCTTCCTCGG CGGCTGAGGC TCTCCGCCTT GGCGGGCGCT 
GGTCGCTTTT GCATTTTAGG GTCTGAAGCG GCGACGCGAA AGCATTTGCC GGCGAGGAAC 
CACTGTGGGC TCTCTGACTC CTCTCCGCAG CTGTGGCCCG AACCGGATTT CAGGAATCCG 
CCAAGGAAGG CGTCTAAGGC CAGCTTAGAC TTTAAGCGTT ACGTAACCGA TCGGAGATTG 
GCTGAGACCC TGGCGCAAAT CTATTTGGGA AAACCAAGTA GACCTCCACA CCTACTGCTG 
GAGTGCAATC CAGGTCCTGG AATCCTGACT CAGGCATTAC TTGAAGCTGG TGCCAAAGTG 
GTTGCGCTCG AAAGTGACAA AACTTTTATT CCACATTTGG AGAAACTAAT GGCAGATCCC 
GGAAATCCAG ACTTGTATCA TGTATTAAGT GTTATCTGGC AATTAGCTTG TGAGATTAAG 
GTTCTGCACA TGGAGCCTTG GTCATCATTT GATATATACA CCCGGAAAGG GCCGCTGGAA 
AACCCAAAGC GTAGGGAATT ATTAGACCAA TTACAACAAA AGCTGTATCT TATTCAAATG 
ATTCCTCGTC AAAATTTATT TACCAAGAAC TTAACACCTA TGAACTATAA TATATTTTTT 
CACTTGTTAA AGCACTGTTT TGGGAGGCGC AGCGCCACTG TAATAGACCA CTTACGTTCA 
TTGACTCCAC TTGATGCGAG AGATATATTG ATGCAAATAG GAAAACAGGA GGATGAGAAA 
GTAGTTAACA TGCACCCTCA AGACTTCAAA ACACTTTTTG AAACTATAGA GCGTTCCAAA 
GATTGTGCTT ATAAATGGCT GTATGATGAA ACCCTGGAAG ATAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TFB2M ( XM_017002055.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857544.1 Homo sapiens transcription factor B2, mitochondrial (TFB2M), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu90382
Clone ID Related Accession (Same CDS sequence)	XM_017002055.1
Accession Version	XM_017002055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dimethyladenosine transferase 2, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAA GTAGAGGTGA GAAAAGGGCA CTTTGGAAAC TCGCATATGA CTTGTATTCC 
TGTACTTCTA TATATAAATT TGGACGAATA GAAGTAAATA TGTTTATTGG TGAAAAAGAA 
TTCCAGAAAC TAATGGCAGA TCCCGGAAAT CCAGACTTGT ATCATGTATT AAGTGTTATC 
TGGCAATTAG CTTGTGAGAT TAAGGTTCTG CACATGGAGC CTTGGTCATC ATTTGATATA 
TACACCCGGA AAGGGCCGCT GGAAAACCCA AAGCGTAGGG AATTATTAGA CCAATTACAA 
CAAAAGCTGT ATCTTATTCA AATGATTCCT CGTCAAAATT TATTTACCAA GAACTTAACA 
CCTATGAACT ATAATATATT TTTTCACTTG TTAAAGCACT GTTTTGGGAG GCGCAGCGCC 
ACTGTAATAG ACCACTTACG TTCATTGACT CCACTTGATG CGAGAGATAT ATTGATGCAA 
ATAGGAAAAC AGGAGGATGA GAAAGTAGTT AACATGCACC CTCAAGACTT CAAAACACTT 
TTTGAAACTA TAGAGCGTTC CAAAGATTGT GCTTATAAAT GGCTGTATGA TGAAACCCTG 
GAAGATAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857544.1
CDS	491..1102
Translation	MFPSRGEKRALWKLAYDLYSCTSIYKFGRIEVNMFIGEKEFQKLMADPGNPDLYHVLSVIWQLACEIKVLHMEPWSSFDIYTRKGPLENPKRRELLDQLQQKLYLIQMIPRQNLFTKNLTPMNYNIFFHLLKHCFGRRSATVIDHLRSLTPLDARDILMQIGKQEDEKVVNMHPQDFKTLFETIERSKDCAYKWLYDETLEDR

Target ORF information:

RefSeq Version	XM_017002055.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transcription factor B2, mitochondrial (TFB2M), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002055.1

ORF Insert Sequence:

ATGTTCCCAA GTAGAGGTGA GAAAAGGGCA CTTTGGAAAC TCGCATATGA CTTGTATTCC 
TGTACTTCTA TATATAAATT TGGACGAATA GAAGTAAATA TGTTTATTGG TGAAAAAGAA 
TTCCAGAAAC TAATGGCAGA TCCCGGAAAT CCAGACTTGT ATCATGTATT AAGTGTTATC 
TGGCAATTAG CTTGTGAGAT TAAGGTTCTG CACATGGAGC CTTGGTCATC ATTTGATATA 
TACACCCGGA AAGGGCCGCT GGAAAACCCA AAGCGTAGGG AATTATTAGA CCAATTACAA 
CAAAAGCTGT ATCTTATTCA AATGATTCCT CGTCAAAATT TATTTACCAA GAACTTAACA 
CCTATGAACT ATAATATATT TTTTCACTTG TTAAAGCACT GTTTTGGGAG GCGCAGCGCC 
ACTGTAATAG ACCACTTACG TTCATTGACT CCACTTGATG CGAGAGATAT ATTGATGCAA 
ATAGGAAAAC AGGAGGATGA GAAAGTAGTT AACATGCACC CTCAAGACTT CAAAACACTT 
TTTGAAACTA TAGAGCGTTC CAAAGATTGT GCTTATAAAT GGCTGTATGA TGAAACCCTG 
GAAGATAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural Basis of Mitochondrial Transcription Initiation.
Cell171(5)1072-1081.e10(2017 Nov)
Hillen HS,Morozov YI,Sarfallah A,Temiakov D,Cramer P

Human Mitochondrial Transcription Factor B2 Is Required for Promoter Melting during Initiation of Transcription.
The Journal of biological chemistry292(7)2637-2645(2017 02)
Posse V,Gustafsson CM

Organization of the human mitochondrial transcription initiation complex.
Nucleic acids research42(6)4100-12(2014 Apr)
Yakubovskaya E,Guja KE,Eng ET,Choi WS,Mejia E,Beglov D,Lukin M,Kozakov D,Garcia-Diaz M

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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TFB2M cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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