ABCG4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ABCG4
Entrez Gene ID	64137
Full Name	ATP binding cassette subfamily G member 4
Synonyms	WHITE2
General protein information	Preferred Names ATP binding cassette subfamily G member 4 Names ATP-binding cassette sub-family G member 4 ATP-binding cassette, sub-family G (WHITE), member 4 putative ABC transporter
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q23.3
Summary	The protein encoded by this gene is a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). The encoded protein is a member of the White subfamily and plays an important role in cellular cholesterol homeostasis. This protein functions as either a homodimer or as a heterodimer with another ABC subfamily protein such as ABCG1. [provided by RefSeq, Jan 2017].
Disorder MIM:	607784

mRNA and Protein(s)

mRNA	Protein	Name
NM_001348191.2	NP_001335120.1	ATP-binding cassette sub-family G member 4 isoform a
NM_001348192.1	NP_001335121.1	ATP-binding cassette sub-family G member 4 isoform b
NM_022169.4	NP_071452.2	ATP-binding cassette sub-family G member 4 isoform a
XM_011542952.2	XP_011541254.1	ATP-binding cassette sub-family G member 4 isoform X1
NM_001142505.1	NP_001135977.1	ATP-binding cassette sub-family G member 4 isoform a
NM_001348191.1	NP_001335120.1	ATP-binding cassette sub-family G member 4 isoform a
NM_001348192.2	NP_001335121.1	ATP-binding cassette sub-family G member 4 isoform b
NM_022169.5	NP_071452.2	ATP-binding cassette sub-family G member 4 isoform a

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ABCG4	NP_001098813.1
Danio rerio (zebrafish)	abcg4b	NP_001104682.1
Arabidopsis thaliana (thale cress)	ABCG14	NP_564383.1
Rattus norvegicus (Norway rat)	Abcg4	NP_001100286.1
Gallus gallus (chicken)	ABCG4	XP_004948104.1
Danio rerio (zebrafish)	abcg4	XP_687685.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001858	XP_321211.4
Pan troglodytes (chimpanzee)	ABCG4	XP_001165264.1
	LOC100429212	XP_002799910.1
Canis lupus familiaris (dog)	ABCG4	XP_005619736.1
Arabidopsis thaliana (thale cress)	AT3G25620	NP_001189973.1
Arabidopsis thaliana (thale cress)	AT4G27420	NP_194472.3
Homo sapiens (human)	ABCG4	NP_071452.2
Mus musculus (house mouse)	Abcg4	NP_620405.3
Drosophila melanogaster (fruit fly)	Atet	NP_001097078.1
Xenopus tropicalis (tropical clawed frog)	abcg4	XP_004916109.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression and function of Abcg4 in the mouse blood-brain barrier: role in restricting the brain entry of amyloid-? peptide.
Dodacki A, Wortman M, Saubam?a B, Chasseigneaux S, Nicolic S, Prince N, Lochus M, Raveu AL, Decl?ves X, Scherrmann JM, Patel SB, Bourasset F
Scientific reports7(1)13393(2017 Oct)

Modulation of miR-185-5p expression by EBV-miR-BART6 contributes to developmental differences in ABCG4 gene expression in human megakaryocytes.
Deng L, Wang X, Jiang L, Yang J, Zhou X, Lu Z, Hu H
The international journal of biochemistry & cell biology81(Pt A)105-111(2016 12)

MicroRNA-463-3p/ABCG4: A new axis in glucose-stimulated insulin secretion.
Hou X, Wu W, Yin B, Liu X, Ren F
Obesity (Silver Spring, Md.)24(11)2368-2376(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Modulation of miR-185-5p expression by EBV-miR-BART6 contributes to developmental differences in ABCG4 gene expression in human megakaryocytes
Title: Modulation of miR-185-5p expression by EBV-miR-BART6 contributes to developmental differences in ABCG4 gene expression in human megakaryocytes.

ABCG1 and ABCG4 alter the distribution of gamma-secretase on the plasma membrane, leading to the decreased gamma-secretase activity and suppressed Abeta secretion
Title: ABCG1 and ABCG4 Suppress ?-Secretase Activity and Amyloid ? Production.

Both the full-length and the short isoforms of ABCG1 can dimerize with ABCG4, whereas the ABCG2 multidrug transporter is unable to form a heterodimer with ABCG4.
Title: Functional Cooperativity between ABCG4 and ABCG1 Isoforms.

HUWE1 and NEDD4-1 are two E3 ligases that are fundamental enzymes in the post-translational regulation of ABCG1 and ABCG4 protein levels and cellular cholesterol export activity
Title: The E3 ubiquitin ligases, HUWE1 and NEDD4-1, are involved in the post-translational regulation of the ABCG1 and ABCG4 lipid transporters.

Study suggests that high ABCG4 expression is associated with poor prognosis in patients with non-small-cell lung cancer with advanced stages.
Title: High ABCG4 Expression Is Associated with Poor Prognosis in Non-Small-Cell Lung Cancer Patients Treated with Cisplatin-Based Chemotherapy.

The following ABCG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ABCG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31976	NM_001142505.1 Latest version!	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu31976	NM_001348191.2 Latest version!	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu31976	NM_022169.5 Latest version!	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu71267	XM_011542952.2 Latest version!	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu102219	NM_001348192.2 Latest version!	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu31976	NM_001348191.1	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu102219	NM_001348192.1	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu31976	NM_022169.4	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ABCG4 ( NM_001348191.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335120.1 Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu31976
Clone ID Related Accession (Same CDS sequence)	NM_001348191.2 , NM_022169.4 , NM_001142505.1 , NM_001348191.1 , NM_022169.5
Accession Version	NM_001348191.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP002956.1 and KF511131.1. On May 31, 2019 this sequence version replaced NM_001348191.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.127059.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGA AGGCGCTGGA GGCCGTGGGC TGTGGACTAG GGCCGGGGGC TGTGGCCATG 
GCCGTGACGC TGGAGGACGG GGCGGAACCC CCTGTGCTGA CCACGCACCT GAAGAAGGTG 
GAGAACCACA TCACTGAAGC CCAGCGCTTC TCCCACCTAC CCAAGCGCTC AGCCGTGGAC 
ATCGAGTTCG TGGAGCTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAAAGGGGT 
TATAAGACCC TTCTCAAGTG CCTCTCAGGT AAATTCTGCC GCCGGGAGCT GATTGGCATC 
ATGGGCCCCT CAGGGGCTGG CAAGTCTACA TTCATGAACA TCTTGGCAGG ATACAGGGAG 
TCTGGAATGA AGGGGCAGAT CCTGGTTAAT GGAAGGCCAC GGGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAAGATGAC ATGCTGCTGC CGCACCTCAC GGTGTTGGAA 
GCCATGATGG TCTCTGCTAA CCTGAAGCTG AGTGAGAAGC AGGAGGTGAA GAAGGAGCTG 
GTGACAGAGA TCCTGACGGC ACTGGGCCTG ATGTCGTGCT CCCACACGAG GACAGCCCTG 
CTCTCTGGCG GGCAGAGGAA GCGTCTGGCC ATCGCCCTGG AGCTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC CACCAGTGGT CTGGATAGCG CCTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC ACAGGGGGGC CGTACCATCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG TCAGTGCATC 
TTCAAAGGCG TGGTCACCAA CCTGATCCCC TATCTAAAGG GACTCGGCTT GCATTGCCCC 
ACCTACCACA ACCCGGCTGA CTTCATCATC GAGGTGGCCT CTGGCGAGTA TGGAGACCTG 
AACCCCATGT TGTTCAGGGC TGTGCAGAAT GGGCTGTGCG CTATGGCTGA GAAGAAGAGC 
AGCCCTGAGA AGAACGAGGT CCCTGCCCCA TGCCCTCCTT GTCCTCCGGA AGTGGATCCC 
ATTGAAAGCC ACACCTTTGC CACCAGCACC CTCACACAGT TCTGCATCCT CTTCAAGAGG 
ACCTTCCTGT CCATCCTCAG GGACACGGTC CTGACCCACC TACGGTTCAT GTCCCACGTG 
GTTATTGGCG TGCTCATCGG CCTCCTCTAC CTGCATATTG GCGACGATGC CAGCAAGGTC 
TTCAACAACA CCGGCTGCCT CTTCTTCTCC ATGCTGTTCC TCATGTTCGC CGCCCTCATG 
CCAACTGTGC TCACCTTCCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CCTCAACTAC 
TGGTACAGCC TCAAAGCGTA TTACCTGGCC AAGACCATGG CTGACGTGCC CTTTCAGGTG 
GTGTGTCCGG TGGTCTACTG CAGCATTGTG TACTGGATGA CGGGCCAGCC CGCTGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCCTGGCC ACCGCCACCG CCTTGGTGGC CCAATCTTTG 
GGGCTGCTGA TCGGAGCTGC TTCCAACTCC CTACAGGTGG CCACTTTTGT GGGCCCAGTT 
ACCGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTTGTCA GCTTCAAGAC CATCCCCACT 
TACCTGCAAT GGAGCTCCTA TCTCTCCTAT GTCAGGTATG GCTTTGAGGG TGTGATCCTG 
ACGATCTATG GCATGGAGCG AGGAGACCTG ACATGTTTAG AGGAACGCTG CCCGTTCCGG 
GAGCCACAGA GCATCCTCCG AGCGCTGGAT GTGGAGGATG CCAAGCTCTA CATGGACTTC 
CTGGTCTTGG GCATCTTCTT CCTAGCCCTG CGGCTGCTGG CCTACCTTGT GCTGCGTTAC 
CGGGTCAAGT CAGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335120.1
CDS	242..2182
Translation	MAEKALEAVGCGLGPGAVAMAVTLEDGAEPPVLTTHLKKVENHITEAQRFSHLPKRSAVDIEFVELSYSVREGPCWRKRGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAQGGRTIICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCAMAEKKSSPEKNEVPAPCPPCPPEVDPIESHTFATSTLTQFCILFKRTFLSILRDTVLTHLRFMSHVVIGVLIGLLYLHIGDDASKVFNNTGCLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYSLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALATATALVAQSLGLLIGAASNSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGVILTIYGMERGDLTCLEERCPFREPQSILRALDVEDAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	NM_001348191.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348191.2

ORF Insert Sequence:

ATGGCGGAGA AGGCGCTGGA GGCCGTGGGC TGTGGACTAG GGCCGGGGGC TGTGGCCATG 
GCCGTGACGC TGGAGGACGG GGCGGAACCC CCTGTGCTGA CCACGCACCT GAAGAAGGTG 
GAGAACCACA TCACTGAAGC CCAGCGCTTC TCCCACCTAC CCAAGCGCTC AGCCGTGGAC 
ATCGAGTTCG TGGAGCTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAAAGGGGT 
TATAAGACCC TTCTCAAGTG CCTCTCAGGT AAATTCTGCC GCCGGGAGCT GATTGGCATC 
ATGGGCCCCT CAGGGGCTGG CAAGTCTACA TTCATGAACA TCTTGGCAGG ATACAGGGAG 
TCTGGAATGA AGGGGCAGAT CCTGGTTAAT GGAAGGCCAC GGGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAAGATGAC ATGCTGCTGC CGCACCTCAC GGTGTTGGAA 
GCCATGATGG TCTCTGCTAA CCTGAAGCTG AGTGAGAAGC AGGAGGTGAA GAAGGAGCTG 
GTGACAGAGA TCCTGACGGC ACTGGGCCTG ATGTCGTGCT CCCACACGAG GACAGCCCTG 
CTCTCTGGCG GGCAGAGGAA GCGTCTGGCC ATCGCCCTGG AGCTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC CACCAGTGGT CTGGATAGCG CCTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC ACAGGGGGGC CGTACCATCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG TCAGTGCATC 
TTCAAAGGCG TGGTCACCAA CCTGATCCCC TATCTAAAGG GACTCGGCTT GCATTGCCCC 
ACCTACCACA ACCCGGCTGA CTTCATCATC GAGGTGGCCT CTGGCGAGTA TGGAGACCTG 
AACCCCATGT TGTTCAGGGC TGTGCAGAAT GGGCTGTGCG CTATGGCTGA GAAGAAGAGC 
AGCCCTGAGA AGAACGAGGT CCCTGCCCCA TGCCCTCCTT GTCCTCCGGA AGTGGATCCC 
ATTGAAAGCC ACACCTTTGC CACCAGCACC CTCACACAGT TCTGCATCCT CTTCAAGAGG 
ACCTTCCTGT CCATCCTCAG GGACACGGTC CTGACCCACC TACGGTTCAT GTCCCACGTG 
GTTATTGGCG TGCTCATCGG CCTCCTCTAC CTGCATATTG GCGACGATGC CAGCAAGGTC 
TTCAACAACA CCGGCTGCCT CTTCTTCTCC ATGCTGTTCC TCATGTTCGC CGCCCTCATG 
CCAACTGTGC TCACCTTCCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CCTCAACTAC 
TGGTACAGCC TCAAAGCGTA TTACCTGGCC AAGACCATGG CTGACGTGCC CTTTCAGGTG 
GTGTGTCCGG TGGTCTACTG CAGCATTGTG TACTGGATGA CGGGCCAGCC CGCTGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCCTGGCC ACCGCCACCG CCTTGGTGGC CCAATCTTTG 
GGGCTGCTGA TCGGAGCTGC TTCCAACTCC CTACAGGTGG CCACTTTTGT GGGCCCAGTT 
ACCGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTTGTCA GCTTCAAGAC CATCCCCACT 
TACCTGCAAT GGAGCTCCTA TCTCTCCTAT GTCAGGTATG GCTTTGAGGG TGTGATCCTG 
ACGATCTATG GCATGGAGCG AGGAGACCTG ACATGTTTAG AGGAACGCTG CCCGTTCCGG 
GAGCCACAGA GCATCCTCCG AGCGCTGGAT GTGGAGGATG CCAAGCTCTA CATGGACTTC 
CTGGTCTTGG GCATCTTCTT CCTAGCCCTG CGGCTGCTGG CCTACCTTGT GCTGCGTTAC 
CGGGTCAAGT CAGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG4 ( NM_022169.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071452.2 Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31976
Clone ID Related Accession (Same CDS sequence)	NM_001348191.2 , NM_022169.4 , NM_001142505.1 , NM_001348191.1 , NM_022169.5
Accession Version	NM_022169.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC041091.1 and DA093690.1. This sequence is a reference standard in the RefSeqGene project. On Dec 5, 2008 this sequence version replaced NM_022169.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC041091.1, SRR1803615.75799.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGA AGGCGCTGGA GGCCGTGGGC TGTGGACTAG GGCCGGGGGC TGTGGCCATG 
GCCGTGACGC TGGAGGACGG GGCGGAACCC CCTGTGCTGA CCACGCACCT GAAGAAGGTG 
GAGAACCACA TCACTGAAGC CCAGCGCTTC TCCCACCTAC CCAAGCGCTC AGCCGTGGAC 
ATCGAGTTCG TGGAGCTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAAAGGGGT 
TATAAGACCC TTCTCAAGTG CCTCTCAGGT AAATTCTGCC GCCGGGAGCT GATTGGCATC 
ATGGGCCCCT CAGGGGCTGG CAAGTCTACA TTCATGAACA TCTTGGCAGG ATACAGGGAG 
TCTGGAATGA AGGGGCAGAT CCTGGTTAAT GGAAGGCCAC GGGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAAGATGAC ATGCTGCTGC CGCACCTCAC GGTGTTGGAA 
GCCATGATGG TCTCTGCTAA CCTGAAGCTG AGTGAGAAGC AGGAGGTGAA GAAGGAGCTG 
GTGACAGAGA TCCTGACGGC ACTGGGCCTG ATGTCGTGCT CCCACACGAG GACAGCCCTG 
CTCTCTGGCG GGCAGAGGAA GCGTCTGGCC ATCGCCCTGG AGCTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC CACCAGTGGT CTGGATAGCG CCTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC ACAGGGGGGC CGTACCATCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG TCAGTGCATC 
TTCAAAGGCG TGGTCACCAA CCTGATCCCC TATCTAAAGG GACTCGGCTT GCATTGCCCC 
ACCTACCACA ACCCGGCTGA CTTCATCATC GAGGTGGCCT CTGGCGAGTA TGGAGACCTG 
AACCCCATGT TGTTCAGGGC TGTGCAGAAT GGGCTGTGCG CTATGGCTGA GAAGAAGAGC 
AGCCCTGAGA AGAACGAGGT CCCTGCCCCA TGCCCTCCTT GTCCTCCGGA AGTGGATCCC 
ATTGAAAGCC ACACCTTTGC CACCAGCACC CTCACACAGT TCTGCATCCT CTTCAAGAGG 
ACCTTCCTGT CCATCCTCAG GGACACGGTC CTGACCCACC TACGGTTCAT GTCCCACGTG 
GTTATTGGCG TGCTCATCGG CCTCCTCTAC CTGCATATTG GCGACGATGC CAGCAAGGTC 
TTCAACAACA CCGGCTGCCT CTTCTTCTCC ATGCTGTTCC TCATGTTCGC CGCCCTCATG 
CCAACTGTGC TCACCTTCCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CCTCAACTAC 
TGGTACAGCC TCAAAGCGTA TTACCTGGCC AAGACCATGG CTGACGTGCC CTTTCAGGTG 
GTGTGTCCGG TGGTCTACTG CAGCATTGTG TACTGGATGA CGGGCCAGCC CGCTGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCCTGGCC ACCGCCACCG CCTTGGTGGC CCAATCTTTG 
GGGCTGCTGA TCGGAGCTGC TTCCAACTCC CTACAGGTGG CCACTTTTGT GGGCCCAGTT 
ACCGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTTGTCA GCTTCAAGAC CATCCCCACT 
TACCTGCAAT GGAGCTCCTA TCTCTCCTAT GTCAGGTATG GCTTTGAGGG TGTGATCCTG 
ACGATCTATG GCATGGAGCG AGGAGACCTG ACATGTTTAG AGGAACGCTG CCCGTTCCGG 
GAGCCACAGA GCATCCTCCG AGCGCTGGAT GTGGAGGATG CCAAGCTCTA CATGGACTTC 
CTGGTCTTGG GCATCTTCTT CCTAGCCCTG CGGCTGCTGG CCTACCTTGT GCTGCGTTAC 
CGGGTCAAGT CAGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071452.2
CDS	337..2277
Translation	MAEKALEAVGCGLGPGAVAMAVTLEDGAEPPVLTTHLKKVENHITEAQRFSHLPKRSAVDIEFVELSYSVREGPCWRKRGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAQGGRTIICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCAMAEKKSSPEKNEVPAPCPPCPPEVDPIESHTFATSTLTQFCILFKRTFLSILRDTVLTHLRFMSHVVIGVLIGLLYLHIGDDASKVFNNTGCLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYSLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALATATALVAQSLGLLIGAASNSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGVILTIYGMERGDLTCLEERCPFREPQSILRALDVEDAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	NM_022169.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022169.4

ORF Insert Sequence:

ATGGCGGAGA AGGCGCTGGA GGCCGTGGGC TGTGGACTAG GGCCGGGGGC TGTGGCCATG 
GCCGTGACGC TGGAGGACGG GGCGGAACCC CCTGTGCTGA CCACGCACCT GAAGAAGGTG 
GAGAACCACA TCACTGAAGC CCAGCGCTTC TCCCACCTAC CCAAGCGCTC AGCCGTGGAC 
ATCGAGTTCG TGGAGCTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAAAGGGGT 
TATAAGACCC TTCTCAAGTG CCTCTCAGGT AAATTCTGCC GCCGGGAGCT GATTGGCATC 
ATGGGCCCCT CAGGGGCTGG CAAGTCTACA TTCATGAACA TCTTGGCAGG ATACAGGGAG 
TCTGGAATGA AGGGGCAGAT CCTGGTTAAT GGAAGGCCAC GGGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAAGATGAC ATGCTGCTGC CGCACCTCAC GGTGTTGGAA 
GCCATGATGG TCTCTGCTAA CCTGAAGCTG AGTGAGAAGC AGGAGGTGAA GAAGGAGCTG 
GTGACAGAGA TCCTGACGGC ACTGGGCCTG ATGTCGTGCT CCCACACGAG GACAGCCCTG 
CTCTCTGGCG GGCAGAGGAA GCGTCTGGCC ATCGCCCTGG AGCTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC CACCAGTGGT CTGGATAGCG CCTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC ACAGGGGGGC CGTACCATCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG TCAGTGCATC 
TTCAAAGGCG TGGTCACCAA CCTGATCCCC TATCTAAAGG GACTCGGCTT GCATTGCCCC 
ACCTACCACA ACCCGGCTGA CTTCATCATC GAGGTGGCCT CTGGCGAGTA TGGAGACCTG 
AACCCCATGT TGTTCAGGGC TGTGCAGAAT GGGCTGTGCG CTATGGCTGA GAAGAAGAGC 
AGCCCTGAGA AGAACGAGGT CCCTGCCCCA TGCCCTCCTT GTCCTCCGGA AGTGGATCCC 
ATTGAAAGCC ACACCTTTGC CACCAGCACC CTCACACAGT TCTGCATCCT CTTCAAGAGG 
ACCTTCCTGT CCATCCTCAG GGACACGGTC CTGACCCACC TACGGTTCAT GTCCCACGTG 
GTTATTGGCG TGCTCATCGG CCTCCTCTAC CTGCATATTG GCGACGATGC CAGCAAGGTC 
TTCAACAACA CCGGCTGCCT CTTCTTCTCC ATGCTGTTCC TCATGTTCGC CGCCCTCATG 
CCAACTGTGC TCACCTTCCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CCTCAACTAC 
TGGTACAGCC TCAAAGCGTA TTACCTGGCC AAGACCATGG CTGACGTGCC CTTTCAGGTG 
GTGTGTCCGG TGGTCTACTG CAGCATTGTG TACTGGATGA CGGGCCAGCC CGCTGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCCTGGCC ACCGCCACCG CCTTGGTGGC CCAATCTTTG 
GGGCTGCTGA TCGGAGCTGC TTCCAACTCC CTACAGGTGG CCACTTTTGT GGGCCCAGTT 
ACCGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTTGTCA GCTTCAAGAC CATCCCCACT 
TACCTGCAAT GGAGCTCCTA TCTCTCCTAT GTCAGGTATG GCTTTGAGGG TGTGATCCTG 
ACGATCTATG GCATGGAGCG AGGAGACCTG ACATGTTTAG AGGAACGCTG CCCGTTCCGG 
GAGCCACAGA GCATCCTCCG AGCGCTGGAT GTGGAGGATG CCAAGCTCTA CATGGACTTC 
CTGGTCTTGG GCATCTTCTT CCTAGCCCTG CGGCTGCTGG CCTACCTTGT GCTGCGTTAC 
CGGGTCAAGT CAGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG4 ( NM_001142505.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135977.1 Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 2, mRNA.

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CloneID	OHu31976
Clone ID Related Accession (Same CDS sequence)	NM_001348191.2 , NM_022169.4 , NM_001142505.1 , NM_001348191.1 , NM_022169.5
Accession Version	NM_001142505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA241043.1 and BC041091.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGA AGGCGCTGGA GGCCGTGGGC TGTGGACTAG GGCCGGGGGC TGTGGCCATG 
GCCGTGACGC TGGAGGACGG GGCGGAACCC CCTGTGCTGA CCACGCACCT GAAGAAGGTG 
GAGAACCACA TCACTGAAGC CCAGCGCTTC TCCCACCTAC CCAAGCGCTC AGCCGTGGAC 
ATCGAGTTCG TGGAGCTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAAAGGGGT 
TATAAGACCC TTCTCAAGTG CCTCTCAGGT AAATTCTGCC GCCGGGAGCT GATTGGCATC 
ATGGGCCCCT CAGGGGCTGG CAAGTCTACA TTCATGAACA TCTTGGCAGG ATACAGGGAG 
TCTGGAATGA AGGGGCAGAT CCTGGTTAAT GGAAGGCCAC GGGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAAGATGAC ATGCTGCTGC CGCACCTCAC GGTGTTGGAA 
GCCATGATGG TCTCTGCTAA CCTGAAGCTG AGTGAGAAGC AGGAGGTGAA GAAGGAGCTG 
GTGACAGAGA TCCTGACGGC ACTGGGCCTG ATGTCGTGCT CCCACACGAG GACAGCCCTG 
CTCTCTGGCG GGCAGAGGAA GCGTCTGGCC ATCGCCCTGG AGCTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC CACCAGTGGT CTGGATAGCG CCTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC ACAGGGGGGC CGTACCATCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG TCAGTGCATC 
TTCAAAGGCG TGGTCACCAA CCTGATCCCC TATCTAAAGG GACTCGGCTT GCATTGCCCC 
ACCTACCACA ACCCGGCTGA CTTCATCATC GAGGTGGCCT CTGGCGAGTA TGGAGACCTG 
AACCCCATGT TGTTCAGGGC TGTGCAGAAT GGGCTGTGCG CTATGGCTGA GAAGAAGAGC 
AGCCCTGAGA AGAACGAGGT CCCTGCCCCA TGCCCTCCTT GTCCTCCGGA AGTGGATCCC 
ATTGAAAGCC ACACCTTTGC CACCAGCACC CTCACACAGT TCTGCATCCT CTTCAAGAGG 
ACCTTCCTGT CCATCCTCAG GGACACGGTC CTGACCCACC TACGGTTCAT GTCCCACGTG 
GTTATTGGCG TGCTCATCGG CCTCCTCTAC CTGCATATTG GCGACGATGC CAGCAAGGTC 
TTCAACAACA CCGGCTGCCT CTTCTTCTCC ATGCTGTTCC TCATGTTCGC CGCCCTCATG 
CCAACTGTGC TCACCTTCCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CCTCAACTAC 
TGGTACAGCC TCAAAGCGTA TTACCTGGCC AAGACCATGG CTGACGTGCC CTTTCAGGTG 
GTGTGTCCGG TGGTCTACTG CAGCATTGTG TACTGGATGA CGGGCCAGCC CGCTGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCCTGGCC ACCGCCACCG CCTTGGTGGC CCAATCTTTG 
GGGCTGCTGA TCGGAGCTGC TTCCAACTCC CTACAGGTGG CCACTTTTGT GGGCCCAGTT 
ACCGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTTGTCA GCTTCAAGAC CATCCCCACT 
TACCTGCAAT GGAGCTCCTA TCTCTCCTAT GTCAGGTATG GCTTTGAGGG TGTGATCCTG 
ACGATCTATG GCATGGAGCG AGGAGACCTG ACATGTTTAG AGGAACGCTG CCCGTTCCGG 
GAGCCACAGA GCATCCTCCG AGCGCTGGAT GTGGAGGATG CCAAGCTCTA CATGGACTTC 
CTGGTCTTGG GCATCTTCTT CCTAGCCCTG CGGCTGCTGG CCTACCTTGT GCTGCGTTAC 
CGGGTCAAGT CAGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135977.1
CDS	113..2053
Translation	MAEKALEAVGCGLGPGAVAMAVTLEDGAEPPVLTTHLKKVENHITEAQRFSHLPKRSAVDIEFVELSYSVREGPCWRKRGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAQGGRTIICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCAMAEKKSSPEKNEVPAPCPPCPPEVDPIESHTFATSTLTQFCILFKRTFLSILRDTVLTHLRFMSHVVIGVLIGLLYLHIGDDASKVFNNTGCLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYSLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALATATALVAQSLGLLIGAASNSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGVILTIYGMERGDLTCLEERCPFREPQSILRALDVEDAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	NM_001142505.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142505.1

ORF Insert Sequence:

ATGGCGGAGA AGGCGCTGGA GGCCGTGGGC TGTGGACTAG GGCCGGGGGC TGTGGCCATG 
GCCGTGACGC TGGAGGACGG GGCGGAACCC CCTGTGCTGA CCACGCACCT GAAGAAGGTG 
GAGAACCACA TCACTGAAGC CCAGCGCTTC TCCCACCTAC CCAAGCGCTC AGCCGTGGAC 
ATCGAGTTCG TGGAGCTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAAAGGGGT 
TATAAGACCC TTCTCAAGTG CCTCTCAGGT AAATTCTGCC GCCGGGAGCT GATTGGCATC 
ATGGGCCCCT CAGGGGCTGG CAAGTCTACA TTCATGAACA TCTTGGCAGG ATACAGGGAG 
TCTGGAATGA AGGGGCAGAT CCTGGTTAAT GGAAGGCCAC GGGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAAGATGAC ATGCTGCTGC CGCACCTCAC GGTGTTGGAA 
GCCATGATGG TCTCTGCTAA CCTGAAGCTG AGTGAGAAGC AGGAGGTGAA GAAGGAGCTG 
GTGACAGAGA TCCTGACGGC ACTGGGCCTG ATGTCGTGCT CCCACACGAG GACAGCCCTG 
CTCTCTGGCG GGCAGAGGAA GCGTCTGGCC ATCGCCCTGG AGCTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC CACCAGTGGT CTGGATAGCG CCTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC ACAGGGGGGC CGTACCATCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG TCAGTGCATC 
TTCAAAGGCG TGGTCACCAA CCTGATCCCC TATCTAAAGG GACTCGGCTT GCATTGCCCC 
ACCTACCACA ACCCGGCTGA CTTCATCATC GAGGTGGCCT CTGGCGAGTA TGGAGACCTG 
AACCCCATGT TGTTCAGGGC TGTGCAGAAT GGGCTGTGCG CTATGGCTGA GAAGAAGAGC 
AGCCCTGAGA AGAACGAGGT CCCTGCCCCA TGCCCTCCTT GTCCTCCGGA AGTGGATCCC 
ATTGAAAGCC ACACCTTTGC CACCAGCACC CTCACACAGT TCTGCATCCT CTTCAAGAGG 
ACCTTCCTGT CCATCCTCAG GGACACGGTC CTGACCCACC TACGGTTCAT GTCCCACGTG 
GTTATTGGCG TGCTCATCGG CCTCCTCTAC CTGCATATTG GCGACGATGC CAGCAAGGTC 
TTCAACAACA CCGGCTGCCT CTTCTTCTCC ATGCTGTTCC TCATGTTCGC CGCCCTCATG 
CCAACTGTGC TCACCTTCCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CCTCAACTAC 
TGGTACAGCC TCAAAGCGTA TTACCTGGCC AAGACCATGG CTGACGTGCC CTTTCAGGTG 
GTGTGTCCGG TGGTCTACTG CAGCATTGTG TACTGGATGA CGGGCCAGCC CGCTGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCCTGGCC ACCGCCACCG CCTTGGTGGC CCAATCTTTG 
GGGCTGCTGA TCGGAGCTGC TTCCAACTCC CTACAGGTGG CCACTTTTGT GGGCCCAGTT 
ACCGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTTGTCA GCTTCAAGAC CATCCCCACT 
TACCTGCAAT GGAGCTCCTA TCTCTCCTAT GTCAGGTATG GCTTTGAGGG TGTGATCCTG 
ACGATCTATG GCATGGAGCG AGGAGACCTG ACATGTTTAG AGGAACGCTG CCCGTTCCGG 
GAGCCACAGA GCATCCTCCG AGCGCTGGAT GTGGAGGATG CCAAGCTCTA CATGGACTTC 
CTGGTCTTGG GCATCTTCTT CCTAGCCCTG CGGCTGCTGG CCTACCTTGT GCTGCGTTAC 
CGGGTCAAGT CAGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG4 ( NM_001348191.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335120.1 Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu31976
Clone ID Related Accession (Same CDS sequence)	NM_001348191.2 , NM_022169.4 , NM_001142505.1 , NM_001348191.1 , NM_022169.5
Accession Version	NM_001348191.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP002956.1 and KF511131.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.127059.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGA AGGCGCTGGA GGCCGTGGGC TGTGGACTAG GGCCGGGGGC TGTGGCCATG 
GCCGTGACGC TGGAGGACGG GGCGGAACCC CCTGTGCTGA CCACGCACCT GAAGAAGGTG 
GAGAACCACA TCACTGAAGC CCAGCGCTTC TCCCACCTAC CCAAGCGCTC AGCCGTGGAC 
ATCGAGTTCG TGGAGCTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAAAGGGGT 
TATAAGACCC TTCTCAAGTG CCTCTCAGGT AAATTCTGCC GCCGGGAGCT GATTGGCATC 
ATGGGCCCCT CAGGGGCTGG CAAGTCTACA TTCATGAACA TCTTGGCAGG ATACAGGGAG 
TCTGGAATGA AGGGGCAGAT CCTGGTTAAT GGAAGGCCAC GGGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAAGATGAC ATGCTGCTGC CGCACCTCAC GGTGTTGGAA 
GCCATGATGG TCTCTGCTAA CCTGAAGCTG AGTGAGAAGC AGGAGGTGAA GAAGGAGCTG 
GTGACAGAGA TCCTGACGGC ACTGGGCCTG ATGTCGTGCT CCCACACGAG GACAGCCCTG 
CTCTCTGGCG GGCAGAGGAA GCGTCTGGCC ATCGCCCTGG AGCTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC CACCAGTGGT CTGGATAGCG CCTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC ACAGGGGGGC CGTACCATCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG TCAGTGCATC 
TTCAAAGGCG TGGTCACCAA CCTGATCCCC TATCTAAAGG GACTCGGCTT GCATTGCCCC 
ACCTACCACA ACCCGGCTGA CTTCATCATC GAGGTGGCCT CTGGCGAGTA TGGAGACCTG 
AACCCCATGT TGTTCAGGGC TGTGCAGAAT GGGCTGTGCG CTATGGCTGA GAAGAAGAGC 
AGCCCTGAGA AGAACGAGGT CCCTGCCCCA TGCCCTCCTT GTCCTCCGGA AGTGGATCCC 
ATTGAAAGCC ACACCTTTGC CACCAGCACC CTCACACAGT TCTGCATCCT CTTCAAGAGG 
ACCTTCCTGT CCATCCTCAG GGACACGGTC CTGACCCACC TACGGTTCAT GTCCCACGTG 
GTTATTGGCG TGCTCATCGG CCTCCTCTAC CTGCATATTG GCGACGATGC CAGCAAGGTC 
TTCAACAACA CCGGCTGCCT CTTCTTCTCC ATGCTGTTCC TCATGTTCGC CGCCCTCATG 
CCAACTGTGC TCACCTTCCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CCTCAACTAC 
TGGTACAGCC TCAAAGCGTA TTACCTGGCC AAGACCATGG CTGACGTGCC CTTTCAGGTG 
GTGTGTCCGG TGGTCTACTG CAGCATTGTG TACTGGATGA CGGGCCAGCC CGCTGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCCTGGCC ACCGCCACCG CCTTGGTGGC CCAATCTTTG 
GGGCTGCTGA TCGGAGCTGC TTCCAACTCC CTACAGGTGG CCACTTTTGT GGGCCCAGTT 
ACCGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTTGTCA GCTTCAAGAC CATCCCCACT 
TACCTGCAAT GGAGCTCCTA TCTCTCCTAT GTCAGGTATG GCTTTGAGGG TGTGATCCTG 
ACGATCTATG GCATGGAGCG AGGAGACCTG ACATGTTTAG AGGAACGCTG CCCGTTCCGG 
GAGCCACAGA GCATCCTCCG AGCGCTGGAT GTGGAGGATG CCAAGCTCTA CATGGACTTC 
CTGGTCTTGG GCATCTTCTT CCTAGCCCTG CGGCTGCTGG CCTACCTTGT GCTGCGTTAC 
CGGGTCAAGT CAGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335120.1
CDS	254..2194
Translation	MAEKALEAVGCGLGPGAVAMAVTLEDGAEPPVLTTHLKKVENHITEAQRFSHLPKRSAVDIEFVELSYSVREGPCWRKRGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAQGGRTIICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCAMAEKKSSPEKNEVPAPCPPCPPEVDPIESHTFATSTLTQFCILFKRTFLSILRDTVLTHLRFMSHVVIGVLIGLLYLHIGDDASKVFNNTGCLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYSLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALATATALVAQSLGLLIGAASNSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGVILTIYGMERGDLTCLEERCPFREPQSILRALDVEDAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	NM_001348191.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348191.1

ORF Insert Sequence:

ATGGCGGAGA AGGCGCTGGA GGCCGTGGGC TGTGGACTAG GGCCGGGGGC TGTGGCCATG 
GCCGTGACGC TGGAGGACGG GGCGGAACCC CCTGTGCTGA CCACGCACCT GAAGAAGGTG 
GAGAACCACA TCACTGAAGC CCAGCGCTTC TCCCACCTAC CCAAGCGCTC AGCCGTGGAC 
ATCGAGTTCG TGGAGCTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAAAGGGGT 
TATAAGACCC TTCTCAAGTG CCTCTCAGGT AAATTCTGCC GCCGGGAGCT GATTGGCATC 
ATGGGCCCCT CAGGGGCTGG CAAGTCTACA TTCATGAACA TCTTGGCAGG ATACAGGGAG 
TCTGGAATGA AGGGGCAGAT CCTGGTTAAT GGAAGGCCAC GGGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAAGATGAC ATGCTGCTGC CGCACCTCAC GGTGTTGGAA 
GCCATGATGG TCTCTGCTAA CCTGAAGCTG AGTGAGAAGC AGGAGGTGAA GAAGGAGCTG 
GTGACAGAGA TCCTGACGGC ACTGGGCCTG ATGTCGTGCT CCCACACGAG GACAGCCCTG 
CTCTCTGGCG GGCAGAGGAA GCGTCTGGCC ATCGCCCTGG AGCTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC CACCAGTGGT CTGGATAGCG CCTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC ACAGGGGGGC CGTACCATCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG TCAGTGCATC 
TTCAAAGGCG TGGTCACCAA CCTGATCCCC TATCTAAAGG GACTCGGCTT GCATTGCCCC 
ACCTACCACA ACCCGGCTGA CTTCATCATC GAGGTGGCCT CTGGCGAGTA TGGAGACCTG 
AACCCCATGT TGTTCAGGGC TGTGCAGAAT GGGCTGTGCG CTATGGCTGA GAAGAAGAGC 
AGCCCTGAGA AGAACGAGGT CCCTGCCCCA TGCCCTCCTT GTCCTCCGGA AGTGGATCCC 
ATTGAAAGCC ACACCTTTGC CACCAGCACC CTCACACAGT TCTGCATCCT CTTCAAGAGG 
ACCTTCCTGT CCATCCTCAG GGACACGGTC CTGACCCACC TACGGTTCAT GTCCCACGTG 
GTTATTGGCG TGCTCATCGG CCTCCTCTAC CTGCATATTG GCGACGATGC CAGCAAGGTC 
TTCAACAACA CCGGCTGCCT CTTCTTCTCC ATGCTGTTCC TCATGTTCGC CGCCCTCATG 
CCAACTGTGC TCACCTTCCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CCTCAACTAC 
TGGTACAGCC TCAAAGCGTA TTACCTGGCC AAGACCATGG CTGACGTGCC CTTTCAGGTG 
GTGTGTCCGG TGGTCTACTG CAGCATTGTG TACTGGATGA CGGGCCAGCC CGCTGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCCTGGCC ACCGCCACCG CCTTGGTGGC CCAATCTTTG 
GGGCTGCTGA TCGGAGCTGC TTCCAACTCC CTACAGGTGG CCACTTTTGT GGGCCCAGTT 
ACCGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTTGTCA GCTTCAAGAC CATCCCCACT 
TACCTGCAAT GGAGCTCCTA TCTCTCCTAT GTCAGGTATG GCTTTGAGGG TGTGATCCTG 
ACGATCTATG GCATGGAGCG AGGAGACCTG ACATGTTTAG AGGAACGCTG CCCGTTCCGG 
GAGCCACAGA GCATCCTCCG AGCGCTGGAT GTGGAGGATG CCAAGCTCTA CATGGACTTC 
CTGGTCTTGG GCATCTTCTT CCTAGCCCTG CGGCTGCTGG CCTACCTTGT GCTGCGTTAC 
CGGGTCAAGT CAGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG4 ( NM_022169.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071452.2 Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31976
Clone ID Related Accession (Same CDS sequence)	NM_001348191.2 , NM_022169.4 , NM_001142505.1 , NM_001348191.1 , NM_022169.5
Accession Version	NM_022169.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC041091.1 and DA093690.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_022169.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC041091.1, SRR1803615.75799.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000619701.5/ ENSP00000481728.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGAGA AGGCGCTGGA GGCCGTGGGC TGTGGACTAG GGCCGGGGGC TGTGGCCATG 
GCCGTGACGC TGGAGGACGG GGCGGAACCC CCTGTGCTGA CCACGCACCT GAAGAAGGTG 
GAGAACCACA TCACTGAAGC CCAGCGCTTC TCCCACCTAC CCAAGCGCTC AGCCGTGGAC 
ATCGAGTTCG TGGAGCTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAAAGGGGT 
TATAAGACCC TTCTCAAGTG CCTCTCAGGT AAATTCTGCC GCCGGGAGCT GATTGGCATC 
ATGGGCCCCT CAGGGGCTGG CAAGTCTACA TTCATGAACA TCTTGGCAGG ATACAGGGAG 
TCTGGAATGA AGGGGCAGAT CCTGGTTAAT GGAAGGCCAC GGGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAAGATGAC ATGCTGCTGC CGCACCTCAC GGTGTTGGAA 
GCCATGATGG TCTCTGCTAA CCTGAAGCTG AGTGAGAAGC AGGAGGTGAA GAAGGAGCTG 
GTGACAGAGA TCCTGACGGC ACTGGGCCTG ATGTCGTGCT CCCACACGAG GACAGCCCTG 
CTCTCTGGCG GGCAGAGGAA GCGTCTGGCC ATCGCCCTGG AGCTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC CACCAGTGGT CTGGATAGCG CCTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC ACAGGGGGGC CGTACCATCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG TCAGTGCATC 
TTCAAAGGCG TGGTCACCAA CCTGATCCCC TATCTAAAGG GACTCGGCTT GCATTGCCCC 
ACCTACCACA ACCCGGCTGA CTTCATCATC GAGGTGGCCT CTGGCGAGTA TGGAGACCTG 
AACCCCATGT TGTTCAGGGC TGTGCAGAAT GGGCTGTGCG CTATGGCTGA GAAGAAGAGC 
AGCCCTGAGA AGAACGAGGT CCCTGCCCCA TGCCCTCCTT GTCCTCCGGA AGTGGATCCC 
ATTGAAAGCC ACACCTTTGC CACCAGCACC CTCACACAGT TCTGCATCCT CTTCAAGAGG 
ACCTTCCTGT CCATCCTCAG GGACACGGTC CTGACCCACC TACGGTTCAT GTCCCACGTG 
GTTATTGGCG TGCTCATCGG CCTCCTCTAC CTGCATATTG GCGACGATGC CAGCAAGGTC 
TTCAACAACA CCGGCTGCCT CTTCTTCTCC ATGCTGTTCC TCATGTTCGC CGCCCTCATG 
CCAACTGTGC TCACCTTCCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CCTCAACTAC 
TGGTACAGCC TCAAAGCGTA TTACCTGGCC AAGACCATGG CTGACGTGCC CTTTCAGGTG 
GTGTGTCCGG TGGTCTACTG CAGCATTGTG TACTGGATGA CGGGCCAGCC CGCTGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCCTGGCC ACCGCCACCG CCTTGGTGGC CCAATCTTTG 
GGGCTGCTGA TCGGAGCTGC TTCCAACTCC CTACAGGTGG CCACTTTTGT GGGCCCAGTT 
ACCGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTTGTCA GCTTCAAGAC CATCCCCACT 
TACCTGCAAT GGAGCTCCTA TCTCTCCTAT GTCAGGTATG GCTTTGAGGG TGTGATCCTG 
ACGATCTATG GCATGGAGCG AGGAGACCTG ACATGTTTAG AGGAACGCTG CCCGTTCCGG 
GAGCCACAGA GCATCCTCCG AGCGCTGGAT GTGGAGGATG CCAAGCTCTA CATGGACTTC 
CTGGTCTTGG GCATCTTCTT CCTAGCCCTG CGGCTGCTGG CCTACCTTGT GCTGCGTTAC 
CGGGTCAAGT CAGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071452.2
CDS	325..2265
Translation	MAEKALEAVGCGLGPGAVAMAVTLEDGAEPPVLTTHLKKVENHITEAQRFSHLPKRSAVDIEFVELSYSVREGPCWRKRGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAQGGRTIICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCAMAEKKSSPEKNEVPAPCPPCPPEVDPIESHTFATSTLTQFCILFKRTFLSILRDTVLTHLRFMSHVVIGVLIGLLYLHIGDDASKVFNNTGCLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYSLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALATATALVAQSLGLLIGAASNSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGVILTIYGMERGDLTCLEERCPFREPQSILRALDVEDAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	NM_022169.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022169.5

ORF Insert Sequence:

ATGGCGGAGA AGGCGCTGGA GGCCGTGGGC TGTGGACTAG GGCCGGGGGC TGTGGCCATG 
GCCGTGACGC TGGAGGACGG GGCGGAACCC CCTGTGCTGA CCACGCACCT GAAGAAGGTG 
GAGAACCACA TCACTGAAGC CCAGCGCTTC TCCCACCTAC CCAAGCGCTC AGCCGTGGAC 
ATCGAGTTCG TGGAGCTGTC CTATTCCGTG CGGGAGGGGC CCTGCTGGCG CAAAAGGGGT 
TATAAGACCC TTCTCAAGTG CCTCTCAGGT AAATTCTGCC GCCGGGAGCT GATTGGCATC 
ATGGGCCCCT CAGGGGCTGG CAAGTCTACA TTCATGAACA TCTTGGCAGG ATACAGGGAG 
TCTGGAATGA AGGGGCAGAT CCTGGTTAAT GGAAGGCCAC GGGAGCTGAG GACCTTCCGC 
AAGATGTCCT GCTACATCAT GCAAGATGAC ATGCTGCTGC CGCACCTCAC GGTGTTGGAA 
GCCATGATGG TCTCTGCTAA CCTGAAGCTG AGTGAGAAGC AGGAGGTGAA GAAGGAGCTG 
GTGACAGAGA TCCTGACGGC ACTGGGCCTG ATGTCGTGCT CCCACACGAG GACAGCCCTG 
CTCTCTGGCG GGCAGAGGAA GCGTCTGGCC ATCGCCCTGG AGCTGGTCAA CAACCCGCCT 
GTCATGTTCT TTGATGAGCC CACCAGTGGT CTGGATAGCG CCTCTTGTTT CCAAGTGGTG 
TCCCTCATGA AGTCCCTGGC ACAGGGGGGC CGTACCATCA TCTGCACCAT CCACCAGCCC 
AGTGCCAAGC TCTTTGAGAT GTTTGACAAG CTCTACATCC TGAGCCAGGG TCAGTGCATC 
TTCAAAGGCG TGGTCACCAA CCTGATCCCC TATCTAAAGG GACTCGGCTT GCATTGCCCC 
ACCTACCACA ACCCGGCTGA CTTCATCATC GAGGTGGCCT CTGGCGAGTA TGGAGACCTG 
AACCCCATGT TGTTCAGGGC TGTGCAGAAT GGGCTGTGCG CTATGGCTGA GAAGAAGAGC 
AGCCCTGAGA AGAACGAGGT CCCTGCCCCA TGCCCTCCTT GTCCTCCGGA AGTGGATCCC 
ATTGAAAGCC ACACCTTTGC CACCAGCACC CTCACACAGT TCTGCATCCT CTTCAAGAGG 
ACCTTCCTGT CCATCCTCAG GGACACGGTC CTGACCCACC TACGGTTCAT GTCCCACGTG 
GTTATTGGCG TGCTCATCGG CCTCCTCTAC CTGCATATTG GCGACGATGC CAGCAAGGTC 
TTCAACAACA CCGGCTGCCT CTTCTTCTCC ATGCTGTTCC TCATGTTCGC CGCCCTCATG 
CCAACTGTGC TCACCTTCCC CTTAGAGATG GCGGTCTTCA TGAGGGAGCA CCTCAACTAC 
TGGTACAGCC TCAAAGCGTA TTACCTGGCC AAGACCATGG CTGACGTGCC CTTTCAGGTG 
GTGTGTCCGG TGGTCTACTG CAGCATTGTG TACTGGATGA CGGGCCAGCC CGCTGAGACC 
AGCCGCTTCC TGCTCTTCTC AGCCCTGGCC ACCGCCACCG CCTTGGTGGC CCAATCTTTG 
GGGCTGCTGA TCGGAGCTGC TTCCAACTCC CTACAGGTGG CCACTTTTGT GGGCCCAGTT 
ACCGCCATCC CTGTCCTCTT GTTCTCCGGC TTCTTTGTCA GCTTCAAGAC CATCCCCACT 
TACCTGCAAT GGAGCTCCTA TCTCTCCTAT GTCAGGTATG GCTTTGAGGG TGTGATCCTG 
ACGATCTATG GCATGGAGCG AGGAGACCTG ACATGTTTAG AGGAACGCTG CCCGTTCCGG 
GAGCCACAGA GCATCCTCCG AGCGCTGGAT GTGGAGGATG CCAAGCTCTA CATGGACTTC 
CTGGTCTTGG GCATCTTCTT CCTAGCCCTG CGGCTGCTGG CCTACCTTGT GCTGCGTTAC 
CGGGTCAAGT CAGAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG4 ( XM_011542952.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541254.1 Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant X1, mRNA.

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CloneID	OHu71267
Clone ID Related Accession (Same CDS sequence)	XM_011542952.2
Accession Version	XM_011542952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGAT TTTCCCACGC CTTTCAGAAG AGGATTGTTG CCTCTGGTTA TAAGACCCTT 
CTCAAGTGCC TCTCAGGTAA ATTCTGCCGC CGGGAGCTGA TTGGCATCAT GGGCCCCTCA 
GGGGCTGGCA AGTCTACATT CATGAACATC TTGGCAGGAT ACAGGGAGTC TGGAATGAAG 
GGGCAGATCC TGGTTAATGG AAGGCCACGG GAGCTGAGGA CCTTCCGCAA GATGTCCTGC 
TACATCATGC AAGATGACAT GCTGCTGCCG CACCTCACGG TGTTGGAAGC CATGATGGTC 
TCTGCTAACC TGAAGCTGAG TGAGAAGCAG GAGGTGAAGA AGGAGCTGGT GACAGAGATC 
CTGACGGCAC TGGGCCTGAT GTCGTGCTCC CACACGAGGA CAGCCCTGCT CTCTGGCGGG 
CAGAGGAAGC GTCTGGCCAT CGCCCTGGAG CTGGTCAACA ACCCGCCTGT CATGTTCTTT 
GATGAGCCCA CCAGTGGTCT GGATAGCGCC TCTTGTTTCC AAGTGGTGTC CCTCATGAAG 
TCCCTGGCAC AGGGGGGCCG TACCATCATC TGCACCATCC ACCAGCCCAG TGCCAAGCTC 
TTTGAGATGT TTGACAAGCT CTACATCCTG AGCCAGGGTC AGTGCATCTT CAAAGGCGTG 
GTCACCAACC TGATCCCCTA TCTAAAGGGA CTCGGCTTGC ATTGCCCCAC CTACCACAAC 
CCGGCTGACT TCATCATCGA GGTGGCCTCT GGCGAGTATG GAGACCTGAA CCCCATGTTG 
TTCAGGGCTG TGCAGAATGG GCTGTGCGCT ATGGCTGAGA AGAAGAGCAG CCCTGAGAAG 
AACGAGGTCC CTGCCCCATG CCCTCCTTGT CCTCCGGAAG TGGATCCCAT TGAAAGCCAC 
ACCTTTGCCA CCAGCACCCT CACACAGTTC TGCATCCTCT TCAAGAGGAC CTTCCTGTCC 
ATCCTCAGGG ACACGGTCCT GACCCACCTA CGGTTCATGT CCCACGTGGT TATTGGCGTG 
CTCATCGGCC TCCTCTACCT GCATATTGGC GACGATGCCA GCAAGGTCTT CAACAACACC 
GGCTGCCTCT TCTTCTCCAT GCTGTTCCTC ATGTTCGCCG CCCTCATGCC AACTGTGCTC 
ACCTTCCCCT TAGAGATGGC GGTCTTCATG AGGGAGCACC TCAACTACTG GTACAGCCTC 
AAAGCGTATT ACCTGGCCAA GACCATGGCT GACGTGCCCT TTCAGGTGGT GTGTCCGGTG 
GTCTACTGCA GCATTGTGTA CTGGATGACG GGCCAGCCCG CTGAGACCAG CCGCTTCCTG 
CTCTTCTCAG CCCTGGCCAC CGCCACCGCC TTGGTGGCCC AATCTTTGGG GCTGCTGATC 
GGAGCTGCTT CCAACTCCCT ACAGGTGGCC ACTTTTGTGG GCCCAGTTAC CGCCATCCCT 
GTCCTCTTGT TCTCCGGCTT CTTTGTCAGC TTCAAGACCA TCCCCACTTA CCTGCAATGG 
AGCTCCTATC TCTCCTATGT CAGGTATGGC TTTGAGGGTG TGATCCTGAC GATCTATGGC 
ATGGAGCGAG GAGACCTGAC ATGTTTAGAG GAACGCTGCC CGTTCCGGGA GCCACAGAGC 
ATCCTCCGAG CGCTGGATGT GGAGGATGCC AAGCTCTACA TGGACTTCCT GGTCTTGGGC 
ATCTTCTTCC TAGCCCTGCG GCTGCTGGCC TACCTTGTGC TGCGTTACCG GGTCAAGTCA 
GAGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541254.1
CDS	557..2305
Translation	MCGFSHAFQKRIVASGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAQGGRTIICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCAMAEKKSSPEKNEVPAPCPPCPPEVDPIESHTFATSTLTQFCILFKRTFLSILRDTVLTHLRFMSHVVIGVLIGLLYLHIGDDASKVFNNTGCLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYSLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALATATALVAQSLGLLIGAASNSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGVILTIYGMERGDLTCLEERCPFREPQSILRALDVEDAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	XM_011542952.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542952.2

ORF Insert Sequence:

ATGTGTGGAT TTTCCCACGC CTTTCAGAAG AGGATTGTTG CCTCTGGTTA TAAGACCCTT 
CTCAAGTGCC TCTCAGGTAA ATTCTGCCGC CGGGAGCTGA TTGGCATCAT GGGCCCCTCA 
GGGGCTGGCA AGTCTACATT CATGAACATC TTGGCAGGAT ACAGGGAGTC TGGAATGAAG 
GGGCAGATCC TGGTTAATGG AAGGCCACGG GAGCTGAGGA CCTTCCGCAA GATGTCCTGC 
TACATCATGC AAGATGACAT GCTGCTGCCG CACCTCACGG TGTTGGAAGC CATGATGGTC 
TCTGCTAACC TGAAGCTGAG TGAGAAGCAG GAGGTGAAGA AGGAGCTGGT GACAGAGATC 
CTGACGGCAC TGGGCCTGAT GTCGTGCTCC CACACGAGGA CAGCCCTGCT CTCTGGCGGG 
CAGAGGAAGC GTCTGGCCAT CGCCCTGGAG CTGGTCAACA ACCCGCCTGT CATGTTCTTT 
GATGAGCCCA CCAGTGGTCT GGATAGCGCC TCTTGTTTCC AAGTGGTGTC CCTCATGAAG 
TCCCTGGCAC AGGGGGGCCG TACCATCATC TGCACCATCC ACCAGCCCAG TGCCAAGCTC 
TTTGAGATGT TTGACAAGCT CTACATCCTG AGCCAGGGTC AGTGCATCTT CAAAGGCGTG 
GTCACCAACC TGATCCCCTA TCTAAAGGGA CTCGGCTTGC ATTGCCCCAC CTACCACAAC 
CCGGCTGACT TCATCATCGA GGTGGCCTCT GGCGAGTATG GAGACCTGAA CCCCATGTTG 
TTCAGGGCTG TGCAGAATGG GCTGTGCGCT ATGGCTGAGA AGAAGAGCAG CCCTGAGAAG 
AACGAGGTCC CTGCCCCATG CCCTCCTTGT CCTCCGGAAG TGGATCCCAT TGAAAGCCAC 
ACCTTTGCCA CCAGCACCCT CACACAGTTC TGCATCCTCT TCAAGAGGAC CTTCCTGTCC 
ATCCTCAGGG ACACGGTCCT GACCCACCTA CGGTTCATGT CCCACGTGGT TATTGGCGTG 
CTCATCGGCC TCCTCTACCT GCATATTGGC GACGATGCCA GCAAGGTCTT CAACAACACC 
GGCTGCCTCT TCTTCTCCAT GCTGTTCCTC ATGTTCGCCG CCCTCATGCC AACTGTGCTC 
ACCTTCCCCT TAGAGATGGC GGTCTTCATG AGGGAGCACC TCAACTACTG GTACAGCCTC 
AAAGCGTATT ACCTGGCCAA GACCATGGCT GACGTGCCCT TTCAGGTGGT GTGTCCGGTG 
GTCTACTGCA GCATTGTGTA CTGGATGACG GGCCAGCCCG CTGAGACCAG CCGCTTCCTG 
CTCTTCTCAG CCCTGGCCAC CGCCACCGCC TTGGTGGCCC AATCTTTGGG GCTGCTGATC 
GGAGCTGCTT CCAACTCCCT ACAGGTGGCC ACTTTTGTGG GCCCAGTTAC CGCCATCCCT 
GTCCTCTTGT TCTCCGGCTT CTTTGTCAGC TTCAAGACCA TCCCCACTTA CCTGCAATGG 
AGCTCCTATC TCTCCTATGT CAGGTATGGC TTTGAGGGTG TGATCCTGAC GATCTATGGC 
ATGGAGCGAG GAGACCTGAC ATGTTTAGAG GAACGCTGCC CGTTCCGGGA GCCACAGAGC 
ATCCTCCGAG CGCTGGATGT GGAGGATGCC AAGCTCTACA TGGACTTCCT GGTCTTGGGC 
ATCTTCTTCC TAGCCCTGCG GCTGCTGGCC TACCTTGTGC TGCGTTACCG GGTCAAGTCA 
GAGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG4 ( NM_001348192.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335121.1 Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 4, mRNA.

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CloneID	OHu102219
Clone ID Related Accession (Same CDS sequence)	NM_001348192.1 , NM_001348192.2
Accession Version	NM_001348192.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 4 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP002956.1 and KF511131.1. On Jan 14, 2017 this sequence version replaced XM_017018121.1. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.133049.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGACC TGGAGCTCAG AGAGGTTAAG CAACTTGCCC GAGGTCACAC AGCTGGTTAT 
AAGACCCTTC TCAAGTGCCT CTCAGGTAAA TTCTGCCGCC GGGAGCTGAT TGGCATCATG 
GGCCCCTCAG GGGCTGGCAA GTCTACATTC ATGAACATCT TGGCAGGATA CAGGGAGTCT 
GGAATGAAGG GGCAGATCCT GGTTAATGGA AGGCCACGGG AGCTGAGGAC CTTCCGCAAG 
ATGTCCTGCT ACATCATGCA AGATGACATG CTGCTGCCGC ACCTCACGGT GTTGGAAGCC 
ATGATGGTCT CTGCTAACCT GAAGCTGAGT GAGAAGCAGG AGGTGAAGAA GGAGCTGGTG 
ACAGAGATCC TGACGGCACT GGGCCTGATG TCGTGCTCCC ACACGAGGAC AGCCCTGCTC 
TCTGGCGGGC AGAGGAAGCG TCTGGCCATC GCCCTGGAGC TGGTCAACAA CCCGCCTGTC 
ATGTTCTTTG ATGAGCCCAC CAGTGGTCTG GATAGCGCCT CTTGTTTCCA AGTGGTGTCC 
CTCATGAAGT CCCTGGCACA GGGGGGCCGT ACCATCATCT GCACCATCCA CCAGCCCAGT 
GCCAAGCTCT TTGAGATGTT TGACAAGCTC TACATCCTGA GCCAGGGTCA GTGCATCTTC 
AAAGGCGTGG TCACCAACCT GATCCCCTAT CTAAAGGGAC TCGGCTTGCA TTGCCCCACC 
TACCACAACC CGGCTGACTT CATCATCGAG GTGGCCTCTG GCGAGTATGG AGACCTGAAC 
CCCATGTTGT TCAGGGCTGT GCAGAATGGG CTGTGCGCTA TGGCTGAGAA GAAGAGCAGC 
CCTGAGAAGA ACGAGGTCCC TGCCCCATGC CCTCCTTGTC CTCCGGAAGT GGATCCCATT 
GAAAGCCACA CCTTTGCCAC CAGCACCCTC ACACAGTTCT GCATCCTCTT CAAGAGGACC 
TTCCTGTCCA TCCTCAGGGA CACGGTCCTG ACCCACCTAC GGTTCATGTC CCACGTGGTT 
ATTGGCGTGC TCATCGGCCT CCTCTACCTG CATATTGGCG ACGATGCCAG CAAGGTCTTC 
AACAACACCG GCTGCCTCTT CTTCTCCATG CTGTTCCTCA TGTTCGCCGC CCTCATGCCA 
ACTGTGCTCA CCTTCCCCTT AGAGATGGCG GTCTTCATGA GGGAGCACCT CAACTACTGG 
TACAGCCTCA AAGCGTATTA CCTGGCCAAG ACCATGGCTG ACGTGCCCTT TCAGGTGGTG 
TGTCCGGTGG TCTACTGCAG CATTGTGTAC TGGATGACGG GCCAGCCCGC TGAGACCAGC 
CGCTTCCTGC TCTTCTCAGC CCTGGCCACC GCCACCGCCT TGGTGGCCCA ATCTTTGGGG 
CTGCTGATCG GAGCTGCTTC CAACTCCCTA CAGGTGGCCA CTTTTGTGGG CCCAGTTACC 
GCCATCCCTG TCCTCTTGTT CTCCGGCTTC TTTGTCAGCT TCAAGACCAT CCCCACTTAC 
CTGCAATGGA GCTCCTATCT CTCCTATGTC AGGTATGGCT TTGAGGGTGT GATCCTGACG 
ATCTATGGCA TGGAGCGAGG AGACCTGACA TGTTTAGAGG AACGCTGCCC GTTCCGGGAG 
CCACAGAGCA TCCTCCGAGC GCTGGATGTG GAGGATGCCA AGCTCTACAT GGACTTCCTG 
GTCTTGGGCA TCTTCTTCCT AGCCCTGCGG CTGCTGGCCT ACCTTGTGCT GCGTTACCGG 
GTCAAGTCAG AGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335121.1
CDS	492..2249
Translation	MRDLELREVKQLARGHTAGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAQGGRTIICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCAMAEKKSSPEKNEVPAPCPPCPPEVDPIESHTFATSTLTQFCILFKRTFLSILRDTVLTHLRFMSHVVIGVLIGLLYLHIGDDASKVFNNTGCLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYSLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALATATALVAQSLGLLIGAASNSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGVILTIYGMERGDLTCLEERCPFREPQSILRALDVEDAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	NM_001348192.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348192.1

ORF Insert Sequence:

ATGAGGGACC TGGAGCTCAG AGAGGTTAAG CAACTTGCCC GAGGTCACAC AGCTGGTTAT 
AAGACCCTTC TCAAGTGCCT CTCAGGTAAA TTCTGCCGCC GGGAGCTGAT TGGCATCATG 
GGCCCCTCAG GGGCTGGCAA GTCTACATTC ATGAACATCT TGGCAGGATA CAGGGAGTCT 
GGAATGAAGG GGCAGATCCT GGTTAATGGA AGGCCACGGG AGCTGAGGAC CTTCCGCAAG 
ATGTCCTGCT ACATCATGCA AGATGACATG CTGCTGCCGC ACCTCACGGT GTTGGAAGCC 
ATGATGGTCT CTGCTAACCT GAAGCTGAGT GAGAAGCAGG AGGTGAAGAA GGAGCTGGTG 
ACAGAGATCC TGACGGCACT GGGCCTGATG TCGTGCTCCC ACACGAGGAC AGCCCTGCTC 
TCTGGCGGGC AGAGGAAGCG TCTGGCCATC GCCCTGGAGC TGGTCAACAA CCCGCCTGTC 
ATGTTCTTTG ATGAGCCCAC CAGTGGTCTG GATAGCGCCT CTTGTTTCCA AGTGGTGTCC 
CTCATGAAGT CCCTGGCACA GGGGGGCCGT ACCATCATCT GCACCATCCA CCAGCCCAGT 
GCCAAGCTCT TTGAGATGTT TGACAAGCTC TACATCCTGA GCCAGGGTCA GTGCATCTTC 
AAAGGCGTGG TCACCAACCT GATCCCCTAT CTAAAGGGAC TCGGCTTGCA TTGCCCCACC 
TACCACAACC CGGCTGACTT CATCATCGAG GTGGCCTCTG GCGAGTATGG AGACCTGAAC 
CCCATGTTGT TCAGGGCTGT GCAGAATGGG CTGTGCGCTA TGGCTGAGAA GAAGAGCAGC 
CCTGAGAAGA ACGAGGTCCC TGCCCCATGC CCTCCTTGTC CTCCGGAAGT GGATCCCATT 
GAAAGCCACA CCTTTGCCAC CAGCACCCTC ACACAGTTCT GCATCCTCTT CAAGAGGACC 
TTCCTGTCCA TCCTCAGGGA CACGGTCCTG ACCCACCTAC GGTTCATGTC CCACGTGGTT 
ATTGGCGTGC TCATCGGCCT CCTCTACCTG CATATTGGCG ACGATGCCAG CAAGGTCTTC 
AACAACACCG GCTGCCTCTT CTTCTCCATG CTGTTCCTCA TGTTCGCCGC CCTCATGCCA 
ACTGTGCTCA CCTTCCCCTT AGAGATGGCG GTCTTCATGA GGGAGCACCT CAACTACTGG 
TACAGCCTCA AAGCGTATTA CCTGGCCAAG ACCATGGCTG ACGTGCCCTT TCAGGTGGTG 
TGTCCGGTGG TCTACTGCAG CATTGTGTAC TGGATGACGG GCCAGCCCGC TGAGACCAGC 
CGCTTCCTGC TCTTCTCAGC CCTGGCCACC GCCACCGCCT TGGTGGCCCA ATCTTTGGGG 
CTGCTGATCG GAGCTGCTTC CAACTCCCTA CAGGTGGCCA CTTTTGTGGG CCCAGTTACC 
GCCATCCCTG TCCTCTTGTT CTCCGGCTTC TTTGTCAGCT TCAAGACCAT CCCCACTTAC 
CTGCAATGGA GCTCCTATCT CTCCTATGTC AGGTATGGCT TTGAGGGTGT GATCCTGACG 
ATCTATGGCA TGGAGCGAGG AGACCTGACA TGTTTAGAGG AACGCTGCCC GTTCCGGGAG 
CCACAGAGCA TCCTCCGAGC GCTGGATGTG GAGGATGCCA AGCTCTACAT GGACTTCCTG 
GTCTTGGGCA TCTTCTTCCT AGCCCTGCGG CTGCTGGCCT ACCTTGTGCT GCGTTACCGG 
GTCAAGTCAG AGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG4 ( NM_001348192.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335121.1 Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 4, mRNA.

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CloneID	OHu102219
Clone ID Related Accession (Same CDS sequence)	NM_001348192.1 , NM_001348192.2
Accession Version	NM_001348192.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 4 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP002956.1 and KF511131.1. On May 31, 2019 this sequence version replaced NM_001348192.1. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.133049.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGACC TGGAGCTCAG AGAGGTTAAG CAACTTGCCC GAGGTCACAC AGCTGGTTAT 
AAGACCCTTC TCAAGTGCCT CTCAGGTAAA TTCTGCCGCC GGGAGCTGAT TGGCATCATG 
GGCCCCTCAG GGGCTGGCAA GTCTACATTC ATGAACATCT TGGCAGGATA CAGGGAGTCT 
GGAATGAAGG GGCAGATCCT GGTTAATGGA AGGCCACGGG AGCTGAGGAC CTTCCGCAAG 
ATGTCCTGCT ACATCATGCA AGATGACATG CTGCTGCCGC ACCTCACGGT GTTGGAAGCC 
ATGATGGTCT CTGCTAACCT GAAGCTGAGT GAGAAGCAGG AGGTGAAGAA GGAGCTGGTG 
ACAGAGATCC TGACGGCACT GGGCCTGATG TCGTGCTCCC ACACGAGGAC AGCCCTGCTC 
TCTGGCGGGC AGAGGAAGCG TCTGGCCATC GCCCTGGAGC TGGTCAACAA CCCGCCTGTC 
ATGTTCTTTG ATGAGCCCAC CAGTGGTCTG GATAGCGCCT CTTGTTTCCA AGTGGTGTCC 
CTCATGAAGT CCCTGGCACA GGGGGGCCGT ACCATCATCT GCACCATCCA CCAGCCCAGT 
GCCAAGCTCT TTGAGATGTT TGACAAGCTC TACATCCTGA GCCAGGGTCA GTGCATCTTC 
AAAGGCGTGG TCACCAACCT GATCCCCTAT CTAAAGGGAC TCGGCTTGCA TTGCCCCACC 
TACCACAACC CGGCTGACTT CATCATCGAG GTGGCCTCTG GCGAGTATGG AGACCTGAAC 
CCCATGTTGT TCAGGGCTGT GCAGAATGGG CTGTGCGCTA TGGCTGAGAA GAAGAGCAGC 
CCTGAGAAGA ACGAGGTCCC TGCCCCATGC CCTCCTTGTC CTCCGGAAGT GGATCCCATT 
GAAAGCCACA CCTTTGCCAC CAGCACCCTC ACACAGTTCT GCATCCTCTT CAAGAGGACC 
TTCCTGTCCA TCCTCAGGGA CACGGTCCTG ACCCACCTAC GGTTCATGTC CCACGTGGTT 
ATTGGCGTGC TCATCGGCCT CCTCTACCTG CATATTGGCG ACGATGCCAG CAAGGTCTTC 
AACAACACCG GCTGCCTCTT CTTCTCCATG CTGTTCCTCA TGTTCGCCGC CCTCATGCCA 
ACTGTGCTCA CCTTCCCCTT AGAGATGGCG GTCTTCATGA GGGAGCACCT CAACTACTGG 
TACAGCCTCA AAGCGTATTA CCTGGCCAAG ACCATGGCTG ACGTGCCCTT TCAGGTGGTG 
TGTCCGGTGG TCTACTGCAG CATTGTGTAC TGGATGACGG GCCAGCCCGC TGAGACCAGC 
CGCTTCCTGC TCTTCTCAGC CCTGGCCACC GCCACCGCCT TGGTGGCCCA ATCTTTGGGG 
CTGCTGATCG GAGCTGCTTC CAACTCCCTA CAGGTGGCCA CTTTTGTGGG CCCAGTTACC 
GCCATCCCTG TCCTCTTGTT CTCCGGCTTC TTTGTCAGCT TCAAGACCAT CCCCACTTAC 
CTGCAATGGA GCTCCTATCT CTCCTATGTC AGGTATGGCT TTGAGGGTGT GATCCTGACG 
ATCTATGGCA TGGAGCGAGG AGACCTGACA TGTTTAGAGG AACGCTGCCC GTTCCGGGAG 
CCACAGAGCA TCCTCCGAGC GCTGGATGTG GAGGATGCCA AGCTCTACAT GGACTTCCTG 
GTCTTGGGCA TCTTCTTCCT AGCCCTGCGG CTGCTGGCCT ACCTTGTGCT GCGTTACCGG 
GTCAAGTCAG AGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335121.1
CDS	480..2237
Translation	MRDLELREVKQLARGHTAGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAQGGRTIICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCAMAEKKSSPEKNEVPAPCPPCPPEVDPIESHTFATSTLTQFCILFKRTFLSILRDTVLTHLRFMSHVVIGVLIGLLYLHIGDDASKVFNNTGCLFFSMLFLMFAALMPTVLTFPLEMAVFMREHLNYWYSLKAYYLAKTMADVPFQVVCPVVYCSIVYWMTGQPAETSRFLLFSALATATALVAQSLGLLIGAASNSLQVATFVGPVTAIPVLLFSGFFVSFKTIPTYLQWSSYLSYVRYGFEGVILTIYGMERGDLTCLEERCPFREPQSILRALDVEDAKLYMDFLVLGIFFLALRLLAYLVLRYRVKSER

Target ORF information:

RefSeq Version	NM_001348192.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 4 (ABCG4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348192.2

ORF Insert Sequence:

ATGAGGGACC TGGAGCTCAG AGAGGTTAAG CAACTTGCCC GAGGTCACAC AGCTGGTTAT 
AAGACCCTTC TCAAGTGCCT CTCAGGTAAA TTCTGCCGCC GGGAGCTGAT TGGCATCATG 
GGCCCCTCAG GGGCTGGCAA GTCTACATTC ATGAACATCT TGGCAGGATA CAGGGAGTCT 
GGAATGAAGG GGCAGATCCT GGTTAATGGA AGGCCACGGG AGCTGAGGAC CTTCCGCAAG 
ATGTCCTGCT ACATCATGCA AGATGACATG CTGCTGCCGC ACCTCACGGT GTTGGAAGCC 
ATGATGGTCT CTGCTAACCT GAAGCTGAGT GAGAAGCAGG AGGTGAAGAA GGAGCTGGTG 
ACAGAGATCC TGACGGCACT GGGCCTGATG TCGTGCTCCC ACACGAGGAC AGCCCTGCTC 
TCTGGCGGGC AGAGGAAGCG TCTGGCCATC GCCCTGGAGC TGGTCAACAA CCCGCCTGTC 
ATGTTCTTTG ATGAGCCCAC CAGTGGTCTG GATAGCGCCT CTTGTTTCCA AGTGGTGTCC 
CTCATGAAGT CCCTGGCACA GGGGGGCCGT ACCATCATCT GCACCATCCA CCAGCCCAGT 
GCCAAGCTCT TTGAGATGTT TGACAAGCTC TACATCCTGA GCCAGGGTCA GTGCATCTTC 
AAAGGCGTGG TCACCAACCT GATCCCCTAT CTAAAGGGAC TCGGCTTGCA TTGCCCCACC 
TACCACAACC CGGCTGACTT CATCATCGAG GTGGCCTCTG GCGAGTATGG AGACCTGAAC 
CCCATGTTGT TCAGGGCTGT GCAGAATGGG CTGTGCGCTA TGGCTGAGAA GAAGAGCAGC 
CCTGAGAAGA ACGAGGTCCC TGCCCCATGC CCTCCTTGTC CTCCGGAAGT GGATCCCATT 
GAAAGCCACA CCTTTGCCAC CAGCACCCTC ACACAGTTCT GCATCCTCTT CAAGAGGACC 
TTCCTGTCCA TCCTCAGGGA CACGGTCCTG ACCCACCTAC GGTTCATGTC CCACGTGGTT 
ATTGGCGTGC TCATCGGCCT CCTCTACCTG CATATTGGCG ACGATGCCAG CAAGGTCTTC 
AACAACACCG GCTGCCTCTT CTTCTCCATG CTGTTCCTCA TGTTCGCCGC CCTCATGCCA 
ACTGTGCTCA CCTTCCCCTT AGAGATGGCG GTCTTCATGA GGGAGCACCT CAACTACTGG 
TACAGCCTCA AAGCGTATTA CCTGGCCAAG ACCATGGCTG ACGTGCCCTT TCAGGTGGTG 
TGTCCGGTGG TCTACTGCAG CATTGTGTAC TGGATGACGG GCCAGCCCGC TGAGACCAGC 
CGCTTCCTGC TCTTCTCAGC CCTGGCCACC GCCACCGCCT TGGTGGCCCA ATCTTTGGGG 
CTGCTGATCG GAGCTGCTTC CAACTCCCTA CAGGTGGCCA CTTTTGTGGG CCCAGTTACC 
GCCATCCCTG TCCTCTTGTT CTCCGGCTTC TTTGTCAGCT TCAAGACCAT CCCCACTTAC 
CTGCAATGGA GCTCCTATCT CTCCTATGTC AGGTATGGCT TTGAGGGTGT GATCCTGACG 
ATCTATGGCA TGGAGCGAGG AGACCTGACA TGTTTAGAGG AACGCTGCCC GTTCCGGGAG 
CCACAGAGCA TCCTCCGAGC GCTGGATGTG GAGGATGCCA AGCTCTACAT GGACTTCCTG 
GTCTTGGGCA TCTTCTTCCT AGCCCTGCGG CTGCTGGCCT ACCTTGTGCT GCGTTACCGG 
GTCAAGTCAG AGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression and function of Abcg4 in the mouse blood-brain barrier: role in restricting the brain entry of amyloid-? peptide.
Scientific reports7(1)13393(2017 Oct)
Dodacki A,Wortman M,Saubam?a B,Chasseigneaux S,Nicolic S,Prince N,Lochus M,Raveu AL,Decl?ves X,Scherrmann JM,Patel SB,Bourasset F

Modulation of miR-185-5p expression by EBV-miR-BART6 contributes to developmental differences in ABCG4 gene expression in human megakaryocytes.
The international journal of biochemistry & cell biology81(Pt A)105-111(2016 12)
Deng L,Wang X,Jiang L,Yang J,Zhou X,Lu Z,Hu H

MicroRNA-463-3p/ABCG4: A new axis in glucose-stimulated insulin secretion.
Obesity (Silver Spring, Md.)24(11)2368-2376(2016 Nov)
Hou X,Wu W,Yin B,Liu X,Ren F

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ABCG4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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