MCCC2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MCCC2
Entrez Gene ID	64087
Full Name	methylcrotonoyl-CoA carboxylase 2
Synonyms	MCCB
General protein information	Preferred Names methylcrotonoyl-CoA carboxylase 2 Names methylcrotonoyl-CoA carboxylase beta chain, mitochondrial 3-methylcrotonyl-CoA carboxylase 2 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta MCCase subunit beta biotin carboxylase methylcrotonoyl-CoA carboxylase 2 (beta) methylcrotonoyl-Coenzyme A carboxylase 2 (beta) non-biotin containing subunit of 3-methylcrotonyl-CoA carboxylase testicular secretory protein Li 29
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q13.2
Summary	This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. [provided by RefSeq, Jul 2008].
Disorder MIM:	609014
Disorder Html:	3-Methylcrotonyl-CoA carboxylase 2 deficiency, 210210 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_022132.4	NP_071415.1	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
NM_022132.5	NP_071415.1	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial isoform 1 precursor
XM_005248567.1	XP_005248624.1	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial isoform X1
XM_011543529.2	XP_011541831.1	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial isoform X3
NM_001363147.1	NP_001350076.1	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial isoform 2 precursor
XM_017009688.1	XP_016865177.1	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial isoform X2

Pathways from BioSystems

Homologies

	MCCC2	XP_001096794.2
Caenorhabditis elegans (roundworm)	F02A9.4	NP_499013.1
Magnaporthe oryzae (rice blast fungus)	MGG_02541	XP_003721260.1
Mus musculus (house mouse)	Mccc2	NP_084302.1
Rattus norvegicus (Norway rat)	Mccc2	NP_001012177.1
Homo sapiens (human)	MCCC2	NP_071415.1
Pan troglodytes (chimpanzee)	MCCC2	XP_517717.2
Danio rerio (zebrafish)	mccc2	NP_998092.1
Oryza sativa (rice)	Os08g0424200	NP_001061832.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002761	XP_312161.3
Neurospora crassa	NCU02127	XP_964285.2
Xenopus tropicalis (tropical clawed frog)	mccc2	NP_001025656.1
Canis lupus familiaris (dog)	MCCC2	XP_535268.2
Bos taurus (cattle)	MCCC2	XP_003587552.1
Gallus gallus (chicken)	MCCC2	XP_424967.3
Drosophila melanogaster (fruit fly)	CG3267	NP_652724.1
Arabidopsis thaliana (thale cress)	MCCB	NP_567950.1

Gene Ontology
Bibliography

Related articles in PubMed

3-Methylcrotonyl-CoA carboxylase deficiency: Mutational spectrum derived from comprehensive newborn screening.
Fonseca H, Azevedo L, Serrano C, Sousa C, Marc?o A, Vilarinho L
Gene594(2)203-210(2016 Dec)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Identification of eight novel mutations and transcript analysis of two splicing mutations in Chinese newborns with MCC deficiency.
Yang L, Yang J, Zhang T, Weng C, Hong F, Tong F, Yang R, Yin X, Yu P, Huang X, Qi M
Clinical genetics88(5)484-8(2015 Nov)

Structure and function of biotin-dependent carboxylases.
Tong L
Cellular and molecular life sciences : CMLS70(5)863-91(2013 Mar)

A single mutation in MCCC1 or MCCC2 as a potential cause of positive screening for 3-methylcrotonyl-CoA carboxylase deficiency.
Morscher RJ, Gr?nert SC, B?rer C, Burda P, Suormala T, Fowler B, Baumgartner MR
Molecular genetics and metabolism105(4)602-6(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study reports data mainly obtained from the Portuguese newborn screening program collected over a ten-year period. Analysis of the MCCC1 and MCCC2 genes yielded 26 previously unreported mutations and a variant of clinically unknown significance.
Title: 3-Methylcrotonyl-CoA carboxylase deficiency: Mutational spectrum derived from comprehensive newborn screening.

Novel mutation in MCCC2 gene was identified in Chinese population.
Title: Identification of eight novel mutations and transcript analysis of two splicing mutations in Chinese newborns with MCC deficiency.

Mutation in 3-methylcrotonyl CoA carboxylase 2 gene is associated with 3-methylcrotonyl-CoA carboxylase deficiency.
Title: A single mutation in MCCC1 or MCCC2 as a potential cause of positive screening for 3-methylcrotonyl-CoA carboxylase deficiency.

study reports eight different mutant alleles of MCCC1 or MCCC2 including six novel mutations in Korean patients with 3-methylcrotonyl-CoA carboxylase (MCC) deficiency
Title: Mutational spectrum in eight Korean patients with 3-methylcrotonyl-CoA carboxylase deficiency.

identified two novel MCCA and four novel MCCB mutant alleles from five MCC-deficient patients
Title: Novel mutations in the human MCCA and MCCB gene causing methylcrotonylglycinuria.

The following MCCC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCCC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24710	NM_022132.5 Latest version!	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu39663	NM_001363147.1 Latest version!	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu39663	XM_005248567.1 Latest version!	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu71230	XM_011543529.2 Latest version!	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu95979	XM_017009688.1 Latest version!	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu24710	NM_022132.4	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCCC2 ( NM_022132.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071415.1 Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), mRNA.

Price & Availability↑

CloneID	OHu24710
Clone ID Related Accession (Same CDS sequence)	NM_022132.4 , NM_022132.5
Accession Version	NM_022132.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC138832.2, AB050049.1, CN309723.1, AC143336.1 and AK025591.1. This sequence is a reference standard in the RefSeqGene project. On Apr 10, 2008 this sequence version replaced NM_022132.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005569.1, SRR1660803.244519.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGATCGCA GTGGTCATGG GCTCCTGCAC CGCAGGAGGA 
GCCTATGTGC CTGCCATGGC TGATGAAAAC ATCATTGTAC GCAAGCAGGG TACCATTTTC 
TTGGCAGGAC CCCCCTTGGT TAAAGCGGCA ACTGGGGAAG AAGTATCTGC TGAGGATCTT 
GGAGGTGCTG ATCTTCATTG CAGAAAGTCT GGAGTAAGTG ACCACTGGGC TTTGGATGAT 
CATCATGCCC TTCACTTAAC TAGGAAGGTT GTGAGGAATC TAAATTATCA GAAGAAATTG 
GATGTCACCA TTGAACCTTC TGAAGAGCCT TTATTTCCTG CTGATGAATT GTATGGAATA 
GTTGGTGCTA ACCTTAAGAG GAGCTTTGAT GTCCGAGAGG TCATTGCTAG AATCGTGGAT 
GGAAGCAGAT TCACTGAGTT CAAAGCCTTT TATGGAGACA CATTAGTTAC AGGATTTGCT 
CGAATATTTG GGTACCCAGT AGGTATCGTT GGAAACAACG GAGTTCTCTT TTCTGAATCT 
GCAAAAAAGG GTACTCACTT TGTCCAGTTA TGCTGCCAAA GAAATATTCC TCTGCTGTTC 
CTTCAAAACA TTACTGGATT TATGGTTGGT AGAGAGTATG AAGCTGAAGG AATTGCCAAG 
GATGGTGCCA AGATGGTGGC CGCTGTGGCC TGTGCCCAAG TGCCTAAGAT AACCCTCATC 
ATTGGGGGCT CCTATGGAGC CGGAAACTAT GGGATGTGTG GCAGAGCATA TAGCCCAAGA 
TTTCTCTACA TTTGGCCAAA TGCTCGTATC TCAGTGATGG GAGGAGAGCA GGCAGCCAAT 
GTGTTGGCCA CGATAACAAA GGACCAAAGA GCCCGGGAAG GAAAGCAGTT CTCCAGTGCT 
GATGAAGCGG CTTTAAAAGA GCCCATCATT AAGAAGTTTG AAGAGGAAGG AAACCCTTAC 
TATTCCAGCG CAAGGGTATG GGATGATGGG ATCATTGATC CAGCAGACAC CAGACTGGTC 
TTGGGTCTCA GTTTTAGTGC AGCCCTCAAC GCACCAATAG AGAAGACTGA CTTCGGTATC 
TTCAGGATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071415.1
CDS	139..1830
Translation	MWAVLRLALRPCARASPAGPRAYHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEKARALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATVKGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVDSGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQIAVVMGSCTAGGAYVPAMADENIIVRKQGTIFLAGPPLVKAATGEEVSAEDLGGADLHCRKSGVSDHWALDDHHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFTEFKAFYGDTLVTGFARIFGYPVGIVGNNGVLFSESAKKGTHFVQLCCQRNIPLLFLQNITGFMVGREYEAEGIAKDGAKMVAAVACAQVPKITLIIGGSYGAGNYGMCGRAYSPRFLYIWPNARISVMGGEQAANVLATITKDQRAREGKQFSSADEAALKEPIIKKFEEEGNPYYSSARVWDDGIIDPADTRLVLGLSFSAALNAPIEKTDFGIFRM

Target ORF information:

RefSeq Version	NM_022132.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022132.4

ORF Insert Sequence:

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGATCGCA GTGGTCATGG GCTCCTGCAC CGCAGGAGGA 
GCCTATGTGC CTGCCATGGC TGATGAAAAC ATCATTGTAC GCAAGCAGGG TACCATTTTC 
TTGGCAGGAC CCCCCTTGGT TAAAGCGGCA ACTGGGGAAG AAGTATCTGC TGAGGATCTT 
GGAGGTGCTG ATCTTCATTG CAGAAAGTCT GGAGTAAGTG ACCACTGGGC TTTGGATGAT 
CATCATGCCC TTCACTTAAC TAGGAAGGTT GTGAGGAATC TAAATTATCA GAAGAAATTG 
GATGTCACCA TTGAACCTTC TGAAGAGCCT TTATTTCCTG CTGATGAATT GTATGGAATA 
GTTGGTGCTA ACCTTAAGAG GAGCTTTGAT GTCCGAGAGG TCATTGCTAG AATCGTGGAT 
GGAAGCAGAT TCACTGAGTT CAAAGCCTTT TATGGAGACA CATTAGTTAC AGGATTTGCT 
CGAATATTTG GGTACCCAGT AGGTATCGTT GGAAACAACG GAGTTCTCTT TTCTGAATCT 
GCAAAAAAGG GTACTCACTT TGTCCAGTTA TGCTGCCAAA GAAATATTCC TCTGCTGTTC 
CTTCAAAACA TTACTGGATT TATGGTTGGT AGAGAGTATG AAGCTGAAGG AATTGCCAAG 
GATGGTGCCA AGATGGTGGC CGCTGTGGCC TGTGCCCAAG TGCCTAAGAT AACCCTCATC 
ATTGGGGGCT CCTATGGAGC CGGAAACTAT GGGATGTGTG GCAGAGCATA TAGCCCAAGA 
TTTCTCTACA TTTGGCCAAA TGCTCGTATC TCAGTGATGG GAGGAGAGCA GGCAGCCAAT 
GTGTTGGCCA CGATAACAAA GGACCAAAGA GCCCGGGAAG GAAAGCAGTT CTCCAGTGCT 
GATGAAGCGG CTTTAAAAGA GCCCATCATT AAGAAGTTTG AAGAGGAAGG AAACCCTTAC 
TATTCCAGCG CAAGGGTATG GGATGATGGG ATCATTGATC CAGCAGACAC CAGACTGGTC 
TTGGGTCTCA GTTTTAGTGC AGCCCTCAAC GCACCAATAG AGAAGACTGA CTTCGGTATC 
TTCAGGATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCCC2 ( NM_022132.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071415.1 Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24710
Clone ID Related Accession (Same CDS sequence)	NM_022132.4 , NM_022132.5
Accession Version	NM_022132.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB050049.1, CN309723.1, AC143336.1 and AK025591.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_022132.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.277580.1, HM005569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000340941.11/ ENSP00000343657.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGATCGCA GTGGTCATGG GCTCCTGCAC CGCAGGAGGA 
GCCTATGTGC CTGCCATGGC TGATGAAAAC ATCATTGTAC GCAAGCAGGG TACCATTTTC 
TTGGCAGGAC CCCCCTTGGT TAAAGCGGCA ACTGGGGAAG AAGTATCTGC TGAGGATCTT 
GGAGGTGCTG ATCTTCATTG CAGAAAGTCT GGAGTAAGTG ACCACTGGGC TTTGGATGAT 
CATCATGCCC TTCACTTAAC TAGGAAGGTT GTGAGGAATC TAAATTATCA GAAGAAATTG 
GATGTCACCA TTGAACCTTC TGAAGAGCCT TTATTTCCTG CTGATGAATT GTATGGAATA 
GTTGGTGCTA ACCTTAAGAG GAGCTTTGAT GTCCGAGAGG TCATTGCTAG AATCGTGGAT 
GGAAGCAGAT TCACTGAGTT CAAAGCCTTT TATGGAGACA CATTAGTTAC AGGATTTGCT 
CGAATATTTG GGTACCCAGT AGGTATCGTT GGAAACAACG GAGTTCTCTT TTCTGAATCT 
GCAAAAAAGG GTACTCACTT TGTCCAGTTA TGCTGCCAAA GAAATATTCC TCTGCTGTTC 
CTTCAAAACA TTACTGGATT TATGGTTGGT AGAGAGTATG AAGCTGAAGG AATTGCCAAG 
GATGGTGCCA AGATGGTGGC CGCTGTGGCC TGTGCCCAAG TGCCTAAGAT AACCCTCATC 
ATTGGGGGCT CCTATGGAGC CGGAAACTAT GGGATGTGTG GCAGAGCATA TAGCCCAAGA 
TTTCTCTACA TTTGGCCAAA TGCTCGTATC TCAGTGATGG GAGGAGAGCA GGCAGCCAAT 
GTGTTGGCCA CGATAACAAA GGACCAAAGA GCCCGGGAAG GAAAGCAGTT CTCCAGTGCT 
GATGAAGCGG CTTTAAAAGA GCCCATCATT AAGAAGTTTG AAGAGGAAGG AAACCCTTAC 
TATTCCAGCG CAAGGGTATG GGATGATGGG ATCATTGATC CAGCAGACAC CAGACTGGTC 
TTGGGTCTCA GTTTTAGTGC AGCCCTCAAC GCACCAATAG AGAAGACTGA CTTCGGTATC 
TTCAGGATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071415.1
CDS	87..1778
Translation	MWAVLRLALRPCARASPAGPRAYHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEKARALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATVKGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVDSGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQIAVVMGSCTAGGAYVPAMADENIIVRKQGTIFLAGPPLVKAATGEEVSAEDLGGADLHCRKSGVSDHWALDDHHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFTEFKAFYGDTLVTGFARIFGYPVGIVGNNGVLFSESAKKGTHFVQLCCQRNIPLLFLQNITGFMVGREYEAEGIAKDGAKMVAAVACAQVPKITLIIGGSYGAGNYGMCGRAYSPRFLYIWPNARISVMGGEQAANVLATITKDQRAREGKQFSSADEAALKEPIIKKFEEEGNPYYSSARVWDDGIIDPADTRLVLGLSFSAALNAPIEKTDFGIFRM

Target ORF information:

RefSeq Version	NM_022132.5
Organism	Homo sapiens(human)
Definition	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022132.5

ORF Insert Sequence:

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGATCGCA GTGGTCATGG GCTCCTGCAC CGCAGGAGGA 
GCCTATGTGC CTGCCATGGC TGATGAAAAC ATCATTGTAC GCAAGCAGGG TACCATTTTC 
TTGGCAGGAC CCCCCTTGGT TAAAGCGGCA ACTGGGGAAG AAGTATCTGC TGAGGATCTT 
GGAGGTGCTG ATCTTCATTG CAGAAAGTCT GGAGTAAGTG ACCACTGGGC TTTGGATGAT 
CATCATGCCC TTCACTTAAC TAGGAAGGTT GTGAGGAATC TAAATTATCA GAAGAAATTG 
GATGTCACCA TTGAACCTTC TGAAGAGCCT TTATTTCCTG CTGATGAATT GTATGGAATA 
GTTGGTGCTA ACCTTAAGAG GAGCTTTGAT GTCCGAGAGG TCATTGCTAG AATCGTGGAT 
GGAAGCAGAT TCACTGAGTT CAAAGCCTTT TATGGAGACA CATTAGTTAC AGGATTTGCT 
CGAATATTTG GGTACCCAGT AGGTATCGTT GGAAACAACG GAGTTCTCTT TTCTGAATCT 
GCAAAAAAGG GTACTCACTT TGTCCAGTTA TGCTGCCAAA GAAATATTCC TCTGCTGTTC 
CTTCAAAACA TTACTGGATT TATGGTTGGT AGAGAGTATG AAGCTGAAGG AATTGCCAAG 
GATGGTGCCA AGATGGTGGC CGCTGTGGCC TGTGCCCAAG TGCCTAAGAT AACCCTCATC 
ATTGGGGGCT CCTATGGAGC CGGAAACTAT GGGATGTGTG GCAGAGCATA TAGCCCAAGA 
TTTCTCTACA TTTGGCCAAA TGCTCGTATC TCAGTGATGG GAGGAGAGCA GGCAGCCAAT 
GTGTTGGCCA CGATAACAAA GGACCAAAGA GCCCGGGAAG GAAAGCAGTT CTCCAGTGCT 
GATGAAGCGG CTTTAAAAGA GCCCATCATT AAGAAGTTTG AAGAGGAAGG AAACCCTTAC 
TATTCCAGCG CAAGGGTATG GGATGATGGG ATCATTGATC CAGCAGACAC CAGACTGGTC 
TTGGGTCTCA GTTTTAGTGC AGCCCTCAAC GCACCAATAG AGAAGACTGA CTTCGGTATC 
TTCAGGATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCCC2 ( NM_001363147.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350076.1 Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu39663
Clone ID Related Accession (Same CDS sequence)	XM_005248567.1 , NM_001363147.1
Accession Version	NM_001363147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC138832.2 and AC143336.1. On May 9, 2018 this sequence version replaced XM_005248567.1. Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1 and encodes a shorter isoform (2) compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.155055.1, SRR1803617.244069.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGGTTAAA GCGGCAACTG GGGAAGAAGT ATCTGCTGAG 
GATCTTGGAG GTGCTGATCT TCATTGCAGA AAGTCTGGAG TAAGTGACCA CTGGGCTTTG 
GATGATCATC ATGCCCTTCA CTTAACTAGG AAGGTTGTGA GGAATCTAAA TTATCAGAAG 
AAATTGGATG TCACCATTGA ACCTTCTGAA GAGCCTTTAT TTCCTGCTGA TGAATTGTAT 
GGAATAGTTG GTGCTAACCT TAAGAGGAGC TTTGATGTCC GAGAGGTCAT TGCTAGAATC 
GTGGATGGAA GCAGATTCAC TGAGTTCAAA GCCTTTTATG GAGACACATT AGTTACAGGA 
TTTGCTCGAA TATTTGGGTA CCCAGTAGGT ATCGTTGGAA ACAACGGAGT TCTCTTTTCT 
GAATCTGCAA AAAAGGGTAC TCACTTTGTC CAGTTATGCT GCCAAAGAAA TATTCCTCTG 
CTGTTCCTTC AAAACATTAC TGGATTTATG GTTGGTAGAG AGTATGAAGC TGAAGGAATT 
GCCAAGGATG GTGCCAAGAT GGTGGCCGCT GTGGCCTGTG CCCAAGTGCC TAAGATAACC 
CTCATCATTG GGGGCTCCTA TGGAGCCGGA AACTATGGGA TGTGTGGCAG AGCATATAGC 
CCAAGATTTC TCTACATTTG GCCAAATGCT CGTATCTCAG TGATGGGAGG AGAGCAGGCA 
GCCAATGTGT TGGCCACGAT AACAAAGGAC CAAAGAGCCC GGGAAGGAAA GCAGTTCTCC 
AGTGCTGATG AAGCGGCTTT AAAAGAGCCC ATCATTAAGA AGTTTGAAGA GGAAGGAAAC 
CCTTACTATT CCAGCGCAAG GGTATGGGAT GATGGGATCA TTGATCCAGC AGACACCAGA 
CTGGTCTTGG GTCTCAGTTT TAGTGCAGCC CTCAACGCAC CAATAGAGAA GACTGACTTC 
GGTATCTTCA GGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350076.1
CDS	87..1664
Translation	MWAVLRLALRPCARASPAGPRAYHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEKARALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATVKGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVDSGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQVKAATGEEVSAEDLGGADLHCRKSGVSDHWALDDHHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFTEFKAFYGDTLVTGFARIFGYPVGIVGNNGVLFSESAKKGTHFVQLCCQRNIPLLFLQNITGFMVGREYEAEGIAKDGAKMVAAVACAQVPKITLIIGGSYGAGNYGMCGRAYSPRFLYIWPNARISVMGGEQAANVLATITKDQRAREGKQFSSADEAALKEPIIKKFEEEGNPYYSSARVWDDGIIDPADTRLVLGLSFSAALNAPIEKTDFGIFRM

Target ORF information:

RefSeq Version	NM_001363147.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363147.1

ORF Insert Sequence:

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGGTTAAA GCGGCAACTG GGGAAGAAGT ATCTGCTGAG 
GATCTTGGAG GTGCTGATCT TCATTGCAGA AAGTCTGGAG TAAGTGACCA CTGGGCTTTG 
GATGATCATC ATGCCCTTCA CTTAACTAGG AAGGTTGTGA GGAATCTAAA TTATCAGAAG 
AAATTGGATG TCACCATTGA ACCTTCTGAA GAGCCTTTAT TTCCTGCTGA TGAATTGTAT 
GGAATAGTTG GTGCTAACCT TAAGAGGAGC TTTGATGTCC GAGAGGTCAT TGCTAGAATC 
GTGGATGGAA GCAGATTCAC TGAGTTCAAA GCCTTTTATG GAGACACATT AGTTACAGGA 
TTTGCTCGAA TATTTGGGTA CCCAGTAGGT ATCGTTGGAA ACAACGGAGT TCTCTTTTCT 
GAATCTGCAA AAAAGGGTAC TCACTTTGTC CAGTTATGCT GCCAAAGAAA TATTCCTCTG 
CTGTTCCTTC AAAACATTAC TGGATTTATG GTTGGTAGAG AGTATGAAGC TGAAGGAATT 
GCCAAGGATG GTGCCAAGAT GGTGGCCGCT GTGGCCTGTG CCCAAGTGCC TAAGATAACC 
CTCATCATTG GGGGCTCCTA TGGAGCCGGA AACTATGGGA TGTGTGGCAG AGCATATAGC 
CCAAGATTTC TCTACATTTG GCCAAATGCT CGTATCTCAG TGATGGGAGG AGAGCAGGCA 
GCCAATGTGT TGGCCACGAT AACAAAGGAC CAAAGAGCCC GGGAAGGAAA GCAGTTCTCC 
AGTGCTGATG AAGCGGCTTT AAAAGAGCCC ATCATTAAGA AGTTTGAAGA GGAAGGAAAC 
CCTTACTATT CCAGCGCAAG GGTATGGGAT GATGGGATCA TTGATCCAGC AGACACCAGA 
CTGGTCTTGG GTCTCAGTTT TAGTGCAGCC CTCAACGCAC CAATAGAGAA GACTGACTTC 
GGTATCTTCA GGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCCC2 ( XM_005248567.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248624.1 Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant X1, mRNA.

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CloneID	OHu39663
Clone ID Related Accession (Same CDS sequence)	XM_005248567.1 , NM_001363147.1
Accession Version	XM_005248567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGGTTAAA GCGGCAACTG GGGAAGAAGT ATCTGCTGAG 
GATCTTGGAG GTGCTGATCT TCATTGCAGA AAGTCTGGAG TAAGTGACCA CTGGGCTTTG 
GATGATCATC ATGCCCTTCA CTTAACTAGG AAGGTTGTGA GGAATCTAAA TTATCAGAAG 
AAATTGGATG TCACCATTGA ACCTTCTGAA GAGCCTTTAT TTCCTGCTGA TGAATTGTAT 
GGAATAGTTG GTGCTAACCT TAAGAGGAGC TTTGATGTCC GAGAGGTCAT TGCTAGAATC 
GTGGATGGAA GCAGATTCAC TGAGTTCAAA GCCTTTTATG GAGACACATT AGTTACAGGA 
TTTGCTCGAA TATTTGGGTA CCCAGTAGGT ATCGTTGGAA ACAACGGAGT TCTCTTTTCT 
GAATCTGCAA AAAAGGGTAC TCACTTTGTC CAGTTATGCT GCCAAAGAAA TATTCCTCTG 
CTGTTCCTTC AAAACATTAC TGGATTTATG GTTGGTAGAG AGTATGAAGC TGAAGGAATT 
GCCAAGGATG GTGCCAAGAT GGTGGCCGCT GTGGCCTGTG CCCAAGTGCC TAAGATAACC 
CTCATCATTG GGGGCTCCTA TGGAGCCGGA AACTATGGGA TGTGTGGCAG AGCATATAGC 
CCAAGATTTC TCTACATTTG GCCAAATGCT CGTATCTCAG TGATGGGAGG AGAGCAGGCA 
GCCAATGTGT TGGCCACGAT AACAAAGGAC CAAAGAGCCC GGGAAGGAAA GCAGTTCTCC 
AGTGCTGATG AAGCGGCTTT AAAAGAGCCC ATCATTAAGA AGTTTGAAGA GGAAGGAAAC 
CCTTACTATT CCAGCGCAAG GGTATGGGAT GATGGGATCA TTGATCCAGC AGACACCAGA 
CTGGTCTTGG GTCTCAGTTT TAGTGCAGCC CTCAACGCAC CAATAGAGAA GACTGACTTC 
GGTATCTTCA GGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248624.1
CDS	41..1618
Translation	MWAVLRLALRPCARASPAGPRAYHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEKARALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATVKGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVDSGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQVKAATGEEVSAEDLGGADLHCRKSGVSDHWALDDHHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFTEFKAFYGDTLVTGFARIFGYPVGIVGNNGVLFSESAKKGTHFVQLCCQRNIPLLFLQNITGFMVGREYEAEGIAKDGAKMVAAVACAQVPKITLIIGGSYGAGNYGMCGRAYSPRFLYIWPNARISVMGGEQAANVLATITKDQRAREGKQFSSADEAALKEPIIKKFEEEGNPYYSSARVWDDGIIDPADTRLVLGLSFSAALNAPIEKTDFGIFRM

Target ORF information:

RefSeq Version	XM_005248567.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248567.1

ORF Insert Sequence:

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGGTTAAA GCGGCAACTG GGGAAGAAGT ATCTGCTGAG 
GATCTTGGAG GTGCTGATCT TCATTGCAGA AAGTCTGGAG TAAGTGACCA CTGGGCTTTG 
GATGATCATC ATGCCCTTCA CTTAACTAGG AAGGTTGTGA GGAATCTAAA TTATCAGAAG 
AAATTGGATG TCACCATTGA ACCTTCTGAA GAGCCTTTAT TTCCTGCTGA TGAATTGTAT 
GGAATAGTTG GTGCTAACCT TAAGAGGAGC TTTGATGTCC GAGAGGTCAT TGCTAGAATC 
GTGGATGGAA GCAGATTCAC TGAGTTCAAA GCCTTTTATG GAGACACATT AGTTACAGGA 
TTTGCTCGAA TATTTGGGTA CCCAGTAGGT ATCGTTGGAA ACAACGGAGT TCTCTTTTCT 
GAATCTGCAA AAAAGGGTAC TCACTTTGTC CAGTTATGCT GCCAAAGAAA TATTCCTCTG 
CTGTTCCTTC AAAACATTAC TGGATTTATG GTTGGTAGAG AGTATGAAGC TGAAGGAATT 
GCCAAGGATG GTGCCAAGAT GGTGGCCGCT GTGGCCTGTG CCCAAGTGCC TAAGATAACC 
CTCATCATTG GGGGCTCCTA TGGAGCCGGA AACTATGGGA TGTGTGGCAG AGCATATAGC 
CCAAGATTTC TCTACATTTG GCCAAATGCT CGTATCTCAG TGATGGGAGG AGAGCAGGCA 
GCCAATGTGT TGGCCACGAT AACAAAGGAC CAAAGAGCCC GGGAAGGAAA GCAGTTCTCC 
AGTGCTGATG AAGCGGCTTT AAAAGAGCCC ATCATTAAGA AGTTTGAAGA GGAAGGAAAC 
CCTTACTATT CCAGCGCAAG GGTATGGGAT GATGGGATCA TTGATCCAGC AGACACCAGA 
CTGGTCTTGG GTCTCAGTTT TAGTGCAGCC CTCAACGCAC CAATAGAGAA GACTGACTTC 
GGTATCTTCA GGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCCC2 ( XM_011543529.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541831.1 Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu71230
Clone ID Related Accession (Same CDS sequence)	XM_011543529.2
Accession Version	XM_011543529.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGATCGCA GTGGTCATGG GCTCCTGCAC CGCAGGAGGA 
GCCTATGTGC CTGCCATGGC TGATGAAAAC ATCATTGTAC GCAAGCAGGG TACCATTTTC 
TTGGCAGGAC CCCCCTTGGT TAAAGCGGCA ACTGGGGAAG AAGTATCTGC TGAGGATCTT 
GGAGGTGCTG ATCTTCATTG CAGAAAGTCT GGAGTAAGTG ACCACTGGGC TTTGGATGAT 
CATCATGCCC TTCACTTAAC TAGGAAGGTT GTGAGGAATC TAAATTATCA GAAGAAATTG 
GATGTCACCA TTGAACCTTC TGAAGAGCCT TTATTTCCTG CTGATGAATT GTATGGAATA 
GTTGGTGCTA ACCTTAAGAG GAGCTTTGAT GTCCGAGAGA AATTCTTCAT GAAATATTTT 
CTACGGCTAG ATCTTAATTC CTATAATAGT ACTTGGCAAC ATGGAAAAGA AGTAAAAAAT 
AATTCAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541831.1
CDS	41..1132
Translation	MWAVLRLALRPCARASPAGPRAYHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEKARALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATVKGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVDSGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQIAVVMGSCTAGGAYVPAMADENIIVRKQGTIFLAGPPLVKAATGEEVSAEDLGGADLHCRKSGVSDHWALDDHHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREKFFMKYFLRLDLNSYNSTWQHGKEVKNNSE

Target ORF information:

RefSeq Version	XM_011543529.2
Organism	Homo sapiens(human)
Definition	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543529.2

ORF Insert Sequence:

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGATCGCA GTGGTCATGG GCTCCTGCAC CGCAGGAGGA 
GCCTATGTGC CTGCCATGGC TGATGAAAAC ATCATTGTAC GCAAGCAGGG TACCATTTTC 
TTGGCAGGAC CCCCCTTGGT TAAAGCGGCA ACTGGGGAAG AAGTATCTGC TGAGGATCTT 
GGAGGTGCTG ATCTTCATTG CAGAAAGTCT GGAGTAAGTG ACCACTGGGC TTTGGATGAT 
CATCATGCCC TTCACTTAAC TAGGAAGGTT GTGAGGAATC TAAATTATCA GAAGAAATTG 
GATGTCACCA TTGAACCTTC TGAAGAGCCT TTATTTCCTG CTGATGAATT GTATGGAATA 
GTTGGTGCTA ACCTTAAGAG GAGCTTTGAT GTCCGAGAGA AATTCTTCAT GAAATATTTT 
CTACGGCTAG ATCTTAATTC CTATAATAGT ACTTGGCAAC ATGGAAAAGA AGTAAAAAAT 
AATTCAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCCC2 ( XM_017009688.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865177.1 Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu95979
Clone ID Related Accession (Same CDS sequence)	XM_017009688.1
Accession Version	XM_017009688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methylcrotonoyl-CoA carboxylase beta chain, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGATCGCA GTGGTCATGG GCTCCTGCAC CGCAGGAGGA 
GCCTATGTGC CTGCCATGGC TGATGAAAAC ATCATTGTAC GCAAGCAGGG TACCATTTTC 
TTGGCAGGAC CCCCCTTGGT TAAAGCGGCA ACTGGGGAAG AAGTATCTGC TGAGGATCTT 
GGAGGTGCTG ATCTTCATTG CAGAAAGTCT GGAGTAAGTG ACCACTGGGC TTTGGATGAT 
CATCATGCCC TTCACTTAAC TAGGAAGGTT GTGAGGAATC TAAATTATCA GAAGAAATTG 
GATGTCACCA TTGAACCTTC TGAAGAGCCT TTATTTCCTG CTGATGAATT GTATGGAATA 
GTTGGTGCTA ACCTTAAGAG GAGCTTTGAT GTCCGAGAGA AATTCTTCAT GAAATATTTT 
CTACGGCTAG ATCTTAATTC CTATAATAGT ACTTGGCAAC ATGCCCTTGG AAAAGCACAA 
GACATAAATC TTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865177.1
CDS	41..1138
Translation	MWAVLRLALRPCARASPAGPRAYHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEKARALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATVKGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVDSGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQIAVVMGSCTAGGAYVPAMADENIIVRKQGTIFLAGPPLVKAATGEEVSAEDLGGADLHCRKSGVSDHWALDDHHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREKFFMKYFLRLDLNSYNSTWQHALGKAQDINLL

Target ORF information:

RefSeq Version	XM_017009688.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methylcrotonoyl-CoA carboxylase 2 (MCCC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009688.1

ORF Insert Sequence:

ATGTGGGCCG TCCTGAGGTT AGCCCTGCGG CCGTGTGCCC GCGCCTCTCC CGCCGGGCCG 
CGCGCCTATC ACGGGGACTC GGTGGCCTCG CTGGGCACCC AGCCGGACTT GGGCTCTGCC 
CTCTACCAGG AGAACTACAA GCAGATGAAA GCACTAGTAA ATCAGCTCCA TGAACGAGTG 
GAGCATATAA AACTAGGAGG TGGTGAGAAA GCCCGAGCAC TTCACATATC AAGAGGAAAA 
CTATTGCCCA GAGAAAGAAT TGACAATCTC ATAGACCCAG GGTCTCCATT TCTGGAATTA 
TCCCAGTTTG CAGGTTACCA GTTATATGAC AATGAGGAGG TGCCAGGAGG TGGCATTATT 
ACAGGCATTG GAAGAGTATC AGGAGTAGAA TGCATGATTA TTGCCAATGA TGCCACCGTC 
AAAGGAGGTG CCTACTACCC AGTGACTGTG AAAAAACAAT TACGGGCCCA AGAAATTGCC 
ATGCAAAACA GGCTCCCCTG CATCTACTTA GTTGATTCGG GAGGAGCATA CTTACCTCGA 
CAAGCAGATG TGTTTCCAGA TCGAGACCAC TTTGGCCGTA CATTCTATAA TCAGGCAATT 
ATGTCTTCTA AAAATATTGC ACAGATCGCA GTGGTCATGG GCTCCTGCAC CGCAGGAGGA 
GCCTATGTGC CTGCCATGGC TGATGAAAAC ATCATTGTAC GCAAGCAGGG TACCATTTTC 
TTGGCAGGAC CCCCCTTGGT TAAAGCGGCA ACTGGGGAAG AAGTATCTGC TGAGGATCTT 
GGAGGTGCTG ATCTTCATTG CAGAAAGTCT GGAGTAAGTG ACCACTGGGC TTTGGATGAT 
CATCATGCCC TTCACTTAAC TAGGAAGGTT GTGAGGAATC TAAATTATCA GAAGAAATTG 
GATGTCACCA TTGAACCTTC TGAAGAGCCT TTATTTCCTG CTGATGAATT GTATGGAATA 
GTTGGTGCTA ACCTTAAGAG GAGCTTTGAT GTCCGAGAGA AATTCTTCAT GAAATATTTT 
CTACGGCTAG ATCTTAATTC CTATAATAGT ACTTGGCAAC ATGCCCTTGG AAAAGCACAA 
GACATAAATC TTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

3-Methylcrotonyl-CoA carboxylase deficiency: Mutational spectrum derived from comprehensive newborn screening.
Gene594(2)203-210(2016 Dec)
Fonseca H,Azevedo L,Serrano C,Sousa C,Marc?o A,Vilarinho L

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Identification of eight novel mutations and transcript analysis of two splicing mutations in Chinese newborns with MCC deficiency.
Clinical genetics88(5)484-8(2015 Nov)
Yang L,Yang J,Zhang T,Weng C,Hong F,Tong F,Yang R,Yin X,Yu P,Huang X,Qi M

Structure and function of biotin-dependent carboxylases.
Cellular and molecular life sciences : CMLS70(5)863-91(2013 Mar)
Tong L

A single mutation in MCCC1 or MCCC2 as a potential cause of positive screening for 3-methylcrotonyl-CoA carboxylase deficiency.
Molecular genetics and metabolism105(4)602-6(2012 Apr)
Morscher RJ,Gr?nert SC,B?rer C,Burda P,Suormala T,Fowler B,Baumgartner MR

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MCCC2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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