MRPS14 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPS14
Entrez Gene ID	63931
Full Name	mitochondrial ribosomal protein S14
Synonyms	DJ262D12.2,HSMRPS14,MRP-S14,S14mt
General protein information	Preferred Names mitochondrial ribosomal protein S14 Names 28S ribosomal protein S14, mitochondrial mitochondrial 28S ribosomal protein S14 mitochondrial small ribosomal subunit protein uS14m
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q25.1
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010].
Disorder MIM:	611978

mRNA and Protein(s)

mRNA	Protein	Name
NM_022100.3	NP_071383.1	28S ribosomal protein S14, mitochondrial
NM_022100.2	NP_071383.1	28S ribosomal protein S14, mitochondrial

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MRPS14	XP_537182.1
Danio rerio (zebrafish)	mrps14	NP_001038823.1
Mus musculus (house mouse)	Mrps14	NP_079750.1
Gallus gallus (chicken)	MRPS14	NP_001264478.1
Homo sapiens (human)	MRPS14	NP_071383.1
Bos taurus (cattle)	MRPS14	NP_001003902.1
Drosophila melanogaster (fruit fly)	mRpS14	NP_728245.1
Xenopus tropicalis (tropical clawed frog)	mrps14	XP_002931484.1
Pan troglodytes (chimpanzee)	MRPS14	XP_001152337.1
	MRPS14	XP_001104412.1
Rattus norvegicus (Norway rat)	Mrps14	NP_001099433.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009077	XP_319827.2

Gene Ontology
Bibliography

Related articles in PubMed

Ribosome. The structure of the human mitochondrial ribosome.
Amunts A, Brown A, Toots J, Scheres SHW, Ramakrishnan V
Science (New York, N.Y.)348(6230)95-98(2015 Apr)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Zhang Z, Gerstein M
Genomics81(5)468-80(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following MRPS14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPS14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09487	NM_022100.3 Latest version!	Homo sapiens mitochondrial ribosomal protein S14 (MRPS14), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu09487	NM_022100.2	Homo sapiens mitochondrial ribosomal protein S14 (MRPS14), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPS14 ( NM_022100.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071383.1 Homo sapiens mitochondrial ribosomal protein S14 (MRPS14), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09487
Clone ID Related Accession (Same CDS sequence)	NM_022100.3 , NM_022100.2
Accession Version	NM_022100.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	28S ribosomal protein S14, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049705.1, BE882322.1, DA610441.1 and AI086885.1. On Nov 23, 2018 this sequence version replaced NM_022100.2. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.278737.1, SRR1803616.6802.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146411, SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000476371.1/ ENSP00000420714.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCCT TCATGCTGGG CTCGCTGCTG CGGACGTTCA AGCAGATGGT TCCTTCATCA 
GCTTCAGGCC AAGTTCGAAG TCACTATGTA GACTGGAGAA TGTGGCGCGA TGTGAAGAGA 
CGAAAAATGG CCTATGAATA CGCAGATGAG AGGCTACGTA TTAATTCACT CAGGAAGAAT 
ACCATTTTGC CAAAAATTCT TCAGGATGTG GCTGATGAAG AAATTGCTGC CCTCCCCCGG 
GATAGCTGTC CTGTTAGAAT CAGAAATCGG TGTGTTATGA CGTCCCGTCC GCGTGGTGTG 
AAGCGGCGCT GGAGGCTTAG TCGTATAGTC TTCCGTCACT TAGCTGACCA TGGGCAACTT 
TCTGGGATCC AGCGAGCGAC ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071383.1
CDS	18..404
Translation	MAAFMLGSLLRTFKQMVPSSASGQVRSHYVDWRMWRDVKRRKMAYEYADERLRINSLRKNTILPKILQDVADEEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHGQLSGIQRATW

Target ORF information:

RefSeq Version	NM_022100.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S14 (MRPS14), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022100.3

ORF Insert Sequence:

ATGGCGGCCT TCATGCTGGG CTCGCTGCTG CGGACGTTCA AGCAGATGGT TCCTTCATCA 
GCTTCAGGCC AAGTTCGAAG TCACTATGTA GACTGGAGAA TGTGGCGCGA TGTGAAGAGA 
CGAAAAATGG CCTATGAATA CGCAGATGAG AGGCTACGTA TTAATTCACT CAGGAAGAAT 
ACCATTTTGC CAAAAATTCT TCAGGATGTG GCTGATGAAG AAATTGCTGC CCTCCCCCGG 
GATAGCTGTC CTGTTAGAAT CAGAAATCGG TGTGTTATGA CGTCCCGTCC GCGTGGTGTG 
AAGCGGCGCT GGAGGCTTAG TCGTATAGTC TTCCGTCACT TAGCTGACCA TGGGCAACTT 
TCTGGGATCC AGCGAGCGAC ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS14 ( NM_022100.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_071383.1 Homo sapiens mitochondrial ribosomal protein S14 (MRPS14), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09487
Clone ID Related Accession (Same CDS sequence)	NM_022100.3 , NM_022100.2
Accession Version	NM_022100.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	28S ribosomal protein S14, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB957578.1, AL049705.1, BE882322.1, DA610441.1 and AI086885.1. On Nov 30, 2010 this sequence version replaced NM_022100.1. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.53861.1, SRR1803615.121705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146411, SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCT TCATGCTGGG CTCGCTGCTG CGGACGTTCA AGCAGATGGT TCCTTCATCA 
GCTTCAGGCC AAGTTCGAAG TCACTATGTA GACTGGAGAA TGTGGCGCGA TGTGAAGAGA 
CGAAAAATGG CCTATGAATA CGCAGATGAG AGGCTACGTA TTAATTCACT CAGGAAGAAT 
ACCATTTTGC CAAAAATTCT TCAGGATGTG GCTGATGAAG AAATTGCTGC CCTCCCCCGG 
GATAGCTGTC CTGTTAGAAT CAGAAATCGG TGTGTTATGA CGTCCCGTCC GCGTGGTGTG 
AAGCGGCGCT GGAGGCTTAG TCGTATAGTC TTCCGTCACT TAGCTGACCA TGGGCAACTT 
TCTGGGATCC AGCGAGCGAC ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071383.1
CDS	48..434
Translation	MAAFMLGSLLRTFKQMVPSSASGQVRSHYVDWRMWRDVKRRKMAYEYADERLRINSLRKNTILPKILQDVADEEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHGQLSGIQRATW

Target ORF information:

RefSeq Version	NM_022100.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S14 (MRPS14), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022100.2

ORF Insert Sequence:

ATGGCGGCCT TCATGCTGGG CTCGCTGCTG CGGACGTTCA AGCAGATGGT TCCTTCATCA 
GCTTCAGGCC AAGTTCGAAG TCACTATGTA GACTGGAGAA TGTGGCGCGA TGTGAAGAGA 
CGAAAAATGG CCTATGAATA CGCAGATGAG AGGCTACGTA TTAATTCACT CAGGAAGAAT 
ACCATTTTGC CAAAAATTCT TCAGGATGTG GCTGATGAAG AAATTGCTGC CCTCCCCCGG 
GATAGCTGTC CTGTTAGAAT CAGAAATCGG TGTGTTATGA CGTCCCGTCC GCGTGGTGTG 
AAGCGGCGCT GGAGGCTTAG TCGTATAGTC TTCCGTCACT TAGCTGACCA TGGGCAACTT 
TCTGGGATCC AGCGAGCGAC ATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ribosome. The structure of the human mitochondrial ribosome.
Science (New York, N.Y.)348(6230)95-98(2015 Apr)
Amunts A,Brown A,Toots J,Scheres SHW,Ramakrishnan V

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Genomics81(5)468-80(2003 May)
Zhang Z,Gerstein M

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MRPS14 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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