BCAN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCAN
Entrez Gene ID	63827
Full Name	brevican
Synonyms	BEHAB,CSPG7
General protein information	Preferred Names brevican Names brevican core protein brain-enriched hyaluronan-binding protein chondroitin sulfate proteoglycan 7 chondroitin sulfate proteoglycan BEHAB
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q23.1
Summary	This gene encodes a member of the lectican family of chondroitin sulfate proteoglycans that is specifically expressed in the central nervous system. This protein is developmentally regulated and may function in the formation of the brain extracellular matrix. This protein is highly expressed in gliomas and may promote the growth and cell motility of brain tumor cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011].
Disorder MIM:	600347

mRNA and Protein(s)

mRNA	Protein	Name
NM_198427.1	NP_940819.1	brevican core protein isoform 2 precursor
NM_021948.5	NP_068767.3	brevican core protein isoform 1 precursor
NM_021948.4	NP_068767.3	brevican core protein isoform 1 precursor
XM_017002047.1	XP_016857536.1	brevican core protein isoform X1
XM_011509866.1	XP_011508168.1	brevican core protein isoform X2
NM_198427.2	NP_940819.1	brevican core protein isoform 2 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BCAN	XP_423655.4
Danio rerio (zebrafish)	bcan	NP_001077282.1
Canis lupus familiaris (dog)	BCAN	XP_005622754.1
Rattus norvegicus (Norway rat)	Bcan	NP_001028837.1
Homo sapiens (human)	BCAN	NP_068767.3
Pan troglodytes (chimpanzee)	BCAN	XP_001166305.1
	BCAN	XP_001116679.1
Xenopus tropicalis (tropical clawed frog)	LOC100486298	XP_002941551.2
Bos taurus (cattle)	BCAN	NP_776421.2
Mus musculus (house mouse)	Bcan	NP_031555.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide methylation screen in low-grade breast cancer identifies novel epigenetically altered genes as potential biomarkers for tumor diagnosis.
Faryna M, Konermann C, Aulmann S, Bermejo JL, Brugger M, Diederichs S, Rom J, Weichenhan D, Claus R, Rehli M, Schirmacher P, Sinn HP, Plass C, Gerhauser C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(12)4937-50(2012 Dec)

Brevican, neurocan, tenascin-C and versican are mainly responsible for the invasiveness of low-grade astrocytoma.
Varga I, Hut?czki G, Szemcs?k CD, Zahuczky G, T?th J, Adamecz Z, Kenyeres A, Bogn?r L, Hanz?ly Z, Klekner A
Pathology oncology research : POR18(2)413-20(2012 Apr)

Candidate gene analysis of the human natural killer-1 carbohydrate pathway and perineuronal nets in schizophrenia: B3GAT2 is associated with disease risk and cortical surface area.
K?hler AK, Djurovic S, Rimol LM, Brown AA, Athanasiu L, J?nsson EG, Hansen T, G?stafsson O, Hall H, Giegling I, Muglia P, Cichon S, Rietschel M, Pietil?inen OP, Peltonen L, Bramon E, Collier D, St Clair D, Sigurdsson E, Petursson H, Rujescu D, Melle I, Werge T, Steen VM, Dale AM, Matthews RT, Agartz I, Andreassen OA
Biological psychiatry69(1)90-6(2011 Jan)

The proteoglycan brevican binds to fibronectin after proteolytic cleavage and promotes glioma cell motility.
Hu B, Kong LL, Matthews RT, Viapiano MS
The Journal of biological chemistry283(36)24848-59(2008 Sep)

Human glioblastomas overexpress ADAMTS-5 that degrades brevican.
Nakada M, Miyamori H, Kita D, Takahashi T, Yamashita J, Sato H, Miura R, Yamaguchi Y, Okada Y
Acta neuropathologica110(3)239-46(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that median methylation levels of BCAN, HOXD1, KCTD8, KLF11, NXPH1, POU4F1, SIM1, and TCF7L1 were >/=30% higher than in normal samples, representing potential biomarkers for tumor diagnosis.
Title: Genome-wide methylation screen in low-grade breast cancer identifies novel epigenetically altered genes as potential biomarkers for tumor diagnosis.

High Brevican is associated with the invasive phenotype of low-grade astrocytoma.
Title: Brevican, neurocan, tenascin-C and versican are mainly responsible for the invasiveness of low-grade astrocytoma.

Observational study and meta-analysis of gene-disease association. (HuGE Navigator)
Title: Candidate gene analysis of the human natural killer-1 carbohydrate pathway and perineuronal nets in schizophrenia: B3GAT2 is associated with disease risk and cortical surface area.

Observational study of gene-disease association. (HuGE Navigator)
Title: Confirmation of genomewide association signals in Chinese Han population reveals risk loci for ischemic stroke.

The proteoglycan brevican binds to fibronectin after proteolytic cleavage and promotes glioma cell motility
Title: The proteoglycan brevican binds to fibronectin after proteolytic cleavage and promotes glioma cell motility.

The following BCAN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCAN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22362	NM_021948.5 Latest version!	Homo sapiens brevican (BCAN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu22362	XM_011509866.1 Latest version!	Homo sapiens brevican (BCAN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$265.30 $379.00
OHu29330	NM_198427.2 Latest version!	Homo sapiens brevican (BCAN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu90378	XM_017002047.1 Latest version!	Homo sapiens brevican (BCAN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu22362	NM_021948.4	Homo sapiens brevican (BCAN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$265.30 $379.00
OHu29330	NM_198427.1	Homo sapiens brevican (BCAN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCAN ( NM_021948.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_068767.3 Homo sapiens brevican (BCAN), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22362
Clone ID Related Accession (Same CDS sequence)	NM_021948.5 , NM_021948.4 , XM_011509866.1
Accession Version	NM_021948.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	brevican core protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY358372.1, BC027971.1, AL365181.24 and AL590666.8. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_021948.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.154141.1, SRR1660809.80700.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000329117.10/ ENSP00000331210.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCAGC TGTTCCTGCC CCTGCTGGCA GCCCTGGTCC TGGCCCAGGC TCCTGCAGCT 
TTAGCAGATG TTCTGGAAGG AGACAGCTCA GAGGACCGCG CTTTTCGCGT GCGCATCGCG 
GGCGACGCGC CACTGCAGGG CGTGCTCGGC GGCGCCCTCA CCATCCCTTG CCACGTCCAC 
TACCTGCGGC CACCGCCGAG CCGCCGGGCT GTGCTGGGCT CTCCGCGGGT CAAGTGGACT 
TTCCTGTCCC GGGGCCGGGA GGCAGAGGTG CTGGTGGCGC GGGGAGTGCG CGTCAAGGTG 
AACGAGGCCT ACCGGTTCCG CGTGGCACTG CCTGCGTACC CAGCGTCGCT CACCGACGTC 
TCCCTGGCGC TGAGCGAGCT GCGCCCCAAC GACTCAGGTA TCTATCGCTG TGAGGTCCAG 
CACGGCATCG ATGACAGCAG CGACGCTGTG GAGGTCAAGG TCAAAGGGGT CGTCTTTCTC 
TACCGAGAGG GCTCTGCCCG CTATGCTTTC TCCTTTTCTG GGGCCCAGGA GGCCTGTGCC 
CGCATTGGAG CCCACATCGC CACCCCGGAG CAGCTCTATG CCGCCTACCT TGGGGGCTAT 
GAGCAATGTG ATGCTGGCTG GCTGTCGGAT CAGACCGTGA GGTATCCCAT CCAGACCCCA 
CGAGAGGCCT GTTACGGAGA CATGGATGGC TTCCCCGGGG TCCGGAACTA TGGTGTGGTG 
GACCCGGATG ACCTCTATGA TGTGTACTGT TATGCTGAAG ACCTAAATGG AGAACTGTTC 
CTGGGTGACC CTCCAGAGAA GCTGACATTG GAGGAAGCAC GGGCGTACTG CCAGGAGCGG 
GGTGCAGAGA TTGCCACCAC GGGCCAACTG TATGCAGCCT GGGATGGTGG CCTGGACCAC 
TGCAGCCCAG GGTGGCTAGC TGATGGCAGT GTGCGCTACC CCATCGTCAC ACCCAGCCAG 
CGCTGTGGTG GGGGCTTGCC TGGTGTCAAG ACTCTCTTCC TCTTCCCCAA CCAGACTGGC 
TTCCCCAATA AGCACAGCCG CTTCAACGTC TACTGCTTCC GAGACTCGGC CCAGCCTTCT 
GCCATCCCTG AGGCCTCCAA CCCAGCCTCC AACCCAGCCT CTGATGGACT AGAGGCTATC 
GTCACAGTGA CAGAGACCCT GGAGGAACTG CAGCTGCCTC AGGAAGCCAC AGAGAGTGAA 
TCCCGTGGGG CCATCTACTC CATCCCCATC ATGGAGGACG GAGGAGGTGG AAGCTCCACT 
CCAGAAGACC CAGCAGAGGC CCCTAGGACG CTCCTAGAAT TTGAAACACA ATCCATGGTA 
CCGCCCACGG GGTTCTCAGA AGAGGAAGGT AAGGCATTGG AGGAAGAAGA GAAATATGAA 
GATGAAGAAG AGAAAGAGGA GGAAGAAGAA GAGGAGGAGG TGGAGGATGA GGCTCTGTGG 
GCATGGCCCA GCGAGCTCAG CAGCCCGGGC CCTGAGGCCT CTCTCCCCAC TGAGCCAGCA 
GCCCAGGAGG AGTCACTCTC CCAGGCGCCA GCAAGGGCAG TCCTGCAGCC TGGTGCATCA 
CCACTTCCTG ATGGAGAGTC AGAAGCTTCC AGGCCTCCAA GGGTCCATGG ACCACCTACT 
GAGACTCTGC CCACTCCCAG GGAGAGGAAC CTAGCATCCC CATCACCTTC CACTCTGGTT 
GAGGCAAGAG AGGTGGGGGA GGCAACTGGT GGTCCTGAGC TATCTGGGGT CCCTCGAGGA 
GAGAGCGAGG AGACAGGAAG CTCCGAGGGT GCCCCTTCCC TGCTTCCAGC CACACGGGCC 
CCTGAGGGTA CCAGGGAGCT GGAGGCCCCC TCTGAAGATA ATTCTGGAAG AACTGCCCCA 
GCAGGGACCT CAGTGCAGGC CCAGCCAGTG CTGCCCACTG ACAGCGCCAG CCGAGGTGGA 
GTGGCCGTGG TCCCCGCATC AGGTGACTGT GTCCCCAGCC CCTGCCACAA TGGTGGGACA 
TGCTTGGAGG AGGAGGAAGG GGTCCGCTGC CTATGTCTGC CTGGCTATGG GGGGGACCTG 
TGCGATGTTG GCCTCCGCTT CTGCAACCCC GGCTGGGACG CCTTCCAGGG CGCCTGCTAC 
AAGCACTTTT CCACACGAAG GAGCTGGGAG GAGGCAGAGA CCCAGTGCCG GATGTACGGC 
GCGCATCTGG CCAGCATCAG CACACCCGAG GAACAGGACT TCATCAACAA CCGGTACCGG 
GAGTACCAGT GGATCGGACT CAACGACAGG ACCATCGAAG GCGACTTCTT GTGGTCGGAT 
GGCGTCCCCC TGCTCTATGA GAACTGGAAC CCTGGGCAGC CTGACAGCTA CTTCCTGTCT 
GGAGAGAACT GCGTGGTCAT GGTGTGGCAT GATCAGGGAC AATGGAGTGA CGTGCCCTGC 
AACTACCACC TGTCCTACAC CTGCAAGATG GGGCTGGTGT CCTGTGGGCC GCCACCGGAG 
CTGCCCCTGG CTCAAGTGTT CGGCCGCCCA CGGCTGCGCT ATGAGGTGGA CACTGTGCTT 
CGCTACCGGT GCCGGGAAGG ACTGGCCCAG CGCAATCTGC CGCTGATCCG ATGCCAAGAG 
AACGGTCGTT GGGAGGCCCC CCAGATCTCC TGTGTGCCCA GAAGACCTGC CCGAGCTCTG 
CACCCAGAGG AGGACCCAGA AGGACGTCAG GGGAGGCTAC TGGGACGCTG GAAGGCGCTG 
TTGATCCCCC CTTCCAGCCC CATGCCAGGT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068767.3
CDS	168..2903
Translation	MAQLFLPLLAALVLAQAPAALADVLEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPAYPASLTDVSLALSELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVYCFRDSAQPSAIPEASNPASNPASDGLEAIVTVTETLEELQLPQEATESESRGAIYSIPIMEDGGGGSSTPEDPAEAPRTLLEFETQSMVPPTGFSEEEGKALEEEEKYEDEEEKEEEEEEEEVEDEALWAWPSELSSPGPEASLPTEPAAQEESLSQAPARAVLQPGASPLPDGESEASRPPRVHGPPTETLPTPRERNLASPSPSTLVEAREVGEATGGPELSGVPRGESEETGSSEGAPSLLPATRAPEGTRELEAPSEDNSGRTAPAGTSVQAQPVLPTDSASRGGVAVVPASGDCVPSPCHNGGTCLEEEEGVRCLCLPGYGGDLCDVGLRFCNPGWDAFQGACYKHFSTRRSWEEAETQCRMYGAHLASISTPEEQDFINNRYREYQWIGLNDRTIEGDFLWSDGVPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQWSDVPCNYHLSYTCKMGLVSCGPPPELPLAQVFGRPRLRYEVDTVLRYRCREGLAQRNLPLIRCQENGRWEAPQISCVPRRPARALHPEEDPEGRQGRLLGRWKALLIPPSSPMPGP

Target ORF information:

RefSeq Version	NM_021948.5
Organism	Homo sapiens(human)
Definition	Homo sapiens brevican (BCAN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021948.5

ORF Insert Sequence:

ATGGCCCAGC TGTTCCTGCC CCTGCTGGCA GCCCTGGTCC TGGCCCAGGC TCCTGCAGCT 
TTAGCAGATG TTCTGGAAGG AGACAGCTCA GAGGACCGCG CTTTTCGCGT GCGCATCGCG 
GGCGACGCGC CACTGCAGGG CGTGCTCGGC GGCGCCCTCA CCATCCCTTG CCACGTCCAC 
TACCTGCGGC CACCGCCGAG CCGCCGGGCT GTGCTGGGCT CTCCGCGGGT CAAGTGGACT 
TTCCTGTCCC GGGGCCGGGA GGCAGAGGTG CTGGTGGCGC GGGGAGTGCG CGTCAAGGTG 
AACGAGGCCT ACCGGTTCCG CGTGGCACTG CCTGCGTACC CAGCGTCGCT CACCGACGTC 
TCCCTGGCGC TGAGCGAGCT GCGCCCCAAC GACTCAGGTA TCTATCGCTG TGAGGTCCAG 
CACGGCATCG ATGACAGCAG CGACGCTGTG GAGGTCAAGG TCAAAGGGGT CGTCTTTCTC 
TACCGAGAGG GCTCTGCCCG CTATGCTTTC TCCTTTTCTG GGGCCCAGGA GGCCTGTGCC 
CGCATTGGAG CCCACATCGC CACCCCGGAG CAGCTCTATG CCGCCTACCT TGGGGGCTAT 
GAGCAATGTG ATGCTGGCTG GCTGTCGGAT CAGACCGTGA GGTATCCCAT CCAGACCCCA 
CGAGAGGCCT GTTACGGAGA CATGGATGGC TTCCCCGGGG TCCGGAACTA TGGTGTGGTG 
GACCCGGATG ACCTCTATGA TGTGTACTGT TATGCTGAAG ACCTAAATGG AGAACTGTTC 
CTGGGTGACC CTCCAGAGAA GCTGACATTG GAGGAAGCAC GGGCGTACTG CCAGGAGCGG 
GGTGCAGAGA TTGCCACCAC GGGCCAACTG TATGCAGCCT GGGATGGTGG CCTGGACCAC 
TGCAGCCCAG GGTGGCTAGC TGATGGCAGT GTGCGCTACC CCATCGTCAC ACCCAGCCAG 
CGCTGTGGTG GGGGCTTGCC TGGTGTCAAG ACTCTCTTCC TCTTCCCCAA CCAGACTGGC 
TTCCCCAATA AGCACAGCCG CTTCAACGTC TACTGCTTCC GAGACTCGGC CCAGCCTTCT 
GCCATCCCTG AGGCCTCCAA CCCAGCCTCC AACCCAGCCT CTGATGGACT AGAGGCTATC 
GTCACAGTGA CAGAGACCCT GGAGGAACTG CAGCTGCCTC AGGAAGCCAC AGAGAGTGAA 
TCCCGTGGGG CCATCTACTC CATCCCCATC ATGGAGGACG GAGGAGGTGG AAGCTCCACT 
CCAGAAGACC CAGCAGAGGC CCCTAGGACG CTCCTAGAAT TTGAAACACA ATCCATGGTA 
CCGCCCACGG GGTTCTCAGA AGAGGAAGGT AAGGCATTGG AGGAAGAAGA GAAATATGAA 
GATGAAGAAG AGAAAGAGGA GGAAGAAGAA GAGGAGGAGG TGGAGGATGA GGCTCTGTGG 
GCATGGCCCA GCGAGCTCAG CAGCCCGGGC CCTGAGGCCT CTCTCCCCAC TGAGCCAGCA 
GCCCAGGAGG AGTCACTCTC CCAGGCGCCA GCAAGGGCAG TCCTGCAGCC TGGTGCATCA 
CCACTTCCTG ATGGAGAGTC AGAAGCTTCC AGGCCTCCAA GGGTCCATGG ACCACCTACT 
GAGACTCTGC CCACTCCCAG GGAGAGGAAC CTAGCATCCC CATCACCTTC CACTCTGGTT 
GAGGCAAGAG AGGTGGGGGA GGCAACTGGT GGTCCTGAGC TATCTGGGGT CCCTCGAGGA 
GAGAGCGAGG AGACAGGAAG CTCCGAGGGT GCCCCTTCCC TGCTTCCAGC CACACGGGCC 
CCTGAGGGTA CCAGGGAGCT GGAGGCCCCC TCTGAAGATA ATTCTGGAAG AACTGCCCCA 
GCAGGGACCT CAGTGCAGGC CCAGCCAGTG CTGCCCACTG ACAGCGCCAG CCGAGGTGGA 
GTGGCCGTGG TCCCCGCATC AGGTGACTGT GTCCCCAGCC CCTGCCACAA TGGTGGGACA 
TGCTTGGAGG AGGAGGAAGG GGTCCGCTGC CTATGTCTGC CTGGCTATGG GGGGGACCTG 
TGCGATGTTG GCCTCCGCTT CTGCAACCCC GGCTGGGACG CCTTCCAGGG CGCCTGCTAC 
AAGCACTTTT CCACACGAAG GAGCTGGGAG GAGGCAGAGA CCCAGTGCCG GATGTACGGC 
GCGCATCTGG CCAGCATCAG CACACCCGAG GAACAGGACT TCATCAACAA CCGGTACCGG 
GAGTACCAGT GGATCGGACT CAACGACAGG ACCATCGAAG GCGACTTCTT GTGGTCGGAT 
GGCGTCCCCC TGCTCTATGA GAACTGGAAC CCTGGGCAGC CTGACAGCTA CTTCCTGTCT 
GGAGAGAACT GCGTGGTCAT GGTGTGGCAT GATCAGGGAC AATGGAGTGA CGTGCCCTGC 
AACTACCACC TGTCCTACAC CTGCAAGATG GGGCTGGTGT CCTGTGGGCC GCCACCGGAG 
CTGCCCCTGG CTCAAGTGTT CGGCCGCCCA CGGCTGCGCT ATGAGGTGGA CACTGTGCTT 
CGCTACCGGT GCCGGGAAGG ACTGGCCCAG CGCAATCTGC CGCTGATCCG ATGCCAAGAG 
AACGGTCGTT GGGAGGCCCC CCAGATCTCC TGTGTGCCCA GAAGACCTGC CCGAGCTCTG 
CACCCAGAGG AGGACCCAGA AGGACGTCAG GGGAGGCTAC TGGGACGCTG GAAGGCGCTG 
TTGATCCCCC CTTCCAGCCC CATGCCAGGT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAN ( NM_021948.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_068767.3 Homo sapiens brevican (BCAN), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22362
Clone ID Related Accession (Same CDS sequence)	NM_021948.5 , NM_021948.4 , XM_011509866.1
Accession Version	NM_021948.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	brevican core protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY358372.1, BC027971.1, AL365181.24, AL590666.8 and DB297352.1. This sequence is a reference standard in the RefSeqGene project. On Sep 21, 2011 this sequence version replaced NM_021948.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358372.1, SRR1660809.75143.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGC TGTTCCTGCC CCTGCTGGCA GCCCTGGTCC TGGCCCAGGC TCCTGCAGCT 
TTAGCAGATG TTCTGGAAGG AGACAGCTCA GAGGACCGCG CTTTTCGCGT GCGCATCGCG 
GGCGACGCGC CACTGCAGGG CGTGCTCGGC GGCGCCCTCA CCATCCCTTG CCACGTCCAC 
TACCTGCGGC CACCGCCGAG CCGCCGGGCT GTGCTGGGCT CTCCGCGGGT CAAGTGGACT 
TTCCTGTCCC GGGGCCGGGA GGCAGAGGTG CTGGTGGCGC GGGGAGTGCG CGTCAAGGTG 
AACGAGGCCT ACCGGTTCCG CGTGGCACTG CCTGCGTACC CAGCGTCGCT CACCGACGTC 
TCCCTGGCGC TGAGCGAGCT GCGCCCCAAC GACTCAGGTA TCTATCGCTG TGAGGTCCAG 
CACGGCATCG ATGACAGCAG CGACGCTGTG GAGGTCAAGG TCAAAGGGGT CGTCTTTCTC 
TACCGAGAGG GCTCTGCCCG CTATGCTTTC TCCTTTTCTG GGGCCCAGGA GGCCTGTGCC 
CGCATTGGAG CCCACATCGC CACCCCGGAG CAGCTCTATG CCGCCTACCT TGGGGGCTAT 
GAGCAATGTG ATGCTGGCTG GCTGTCGGAT CAGACCGTGA GGTATCCCAT CCAGACCCCA 
CGAGAGGCCT GTTACGGAGA CATGGATGGC TTCCCCGGGG TCCGGAACTA TGGTGTGGTG 
GACCCGGATG ACCTCTATGA TGTGTACTGT TATGCTGAAG ACCTAAATGG AGAACTGTTC 
CTGGGTGACC CTCCAGAGAA GCTGACATTG GAGGAAGCAC GGGCGTACTG CCAGGAGCGG 
GGTGCAGAGA TTGCCACCAC GGGCCAACTG TATGCAGCCT GGGATGGTGG CCTGGACCAC 
TGCAGCCCAG GGTGGCTAGC TGATGGCAGT GTGCGCTACC CCATCGTCAC ACCCAGCCAG 
CGCTGTGGTG GGGGCTTGCC TGGTGTCAAG ACTCTCTTCC TCTTCCCCAA CCAGACTGGC 
TTCCCCAATA AGCACAGCCG CTTCAACGTC TACTGCTTCC GAGACTCGGC CCAGCCTTCT 
GCCATCCCTG AGGCCTCCAA CCCAGCCTCC AACCCAGCCT CTGATGGACT AGAGGCTATC 
GTCACAGTGA CAGAGACCCT GGAGGAACTG CAGCTGCCTC AGGAAGCCAC AGAGAGTGAA 
TCCCGTGGGG CCATCTACTC CATCCCCATC ATGGAGGACG GAGGAGGTGG AAGCTCCACT 
CCAGAAGACC CAGCAGAGGC CCCTAGGACG CTCCTAGAAT TTGAAACACA ATCCATGGTA 
CCGCCCACGG GGTTCTCAGA AGAGGAAGGT AAGGCATTGG AGGAAGAAGA GAAATATGAA 
GATGAAGAAG AGAAAGAGGA GGAAGAAGAA GAGGAGGAGG TGGAGGATGA GGCTCTGTGG 
GCATGGCCCA GCGAGCTCAG CAGCCCGGGC CCTGAGGCCT CTCTCCCCAC TGAGCCAGCA 
GCCCAGGAGG AGTCACTCTC CCAGGCGCCA GCAAGGGCAG TCCTGCAGCC TGGTGCATCA 
CCACTTCCTG ATGGAGAGTC AGAAGCTTCC AGGCCTCCAA GGGTCCATGG ACCACCTACT 
GAGACTCTGC CCACTCCCAG GGAGAGGAAC CTAGCATCCC CATCACCTTC CACTCTGGTT 
GAGGCAAGAG AGGTGGGGGA GGCAACTGGT GGTCCTGAGC TATCTGGGGT CCCTCGAGGA 
GAGAGCGAGG AGACAGGAAG CTCCGAGGGT GCCCCTTCCC TGCTTCCAGC CACACGGGCC 
CCTGAGGGTA CCAGGGAGCT GGAGGCCCCC TCTGAAGATA ATTCTGGAAG AACTGCCCCA 
GCAGGGACCT CAGTGCAGGC CCAGCCAGTG CTGCCCACTG ACAGCGCCAG CCGAGGTGGA 
GTGGCCGTGG TCCCCGCATC AGGTGACTGT GTCCCCAGCC CCTGCCACAA TGGTGGGACA 
TGCTTGGAGG AGGAGGAAGG GGTCCGCTGC CTATGTCTGC CTGGCTATGG GGGGGACCTG 
TGCGATGTTG GCCTCCGCTT CTGCAACCCC GGCTGGGACG CCTTCCAGGG CGCCTGCTAC 
AAGCACTTTT CCACACGAAG GAGCTGGGAG GAGGCAGAGA CCCAGTGCCG GATGTACGGC 
GCGCATCTGG CCAGCATCAG CACACCCGAG GAACAGGACT TCATCAACAA CCGGTACCGG 
GAGTACCAGT GGATCGGACT CAACGACAGG ACCATCGAAG GCGACTTCTT GTGGTCGGAT 
GGCGTCCCCC TGCTCTATGA GAACTGGAAC CCTGGGCAGC CTGACAGCTA CTTCCTGTCT 
GGAGAGAACT GCGTGGTCAT GGTGTGGCAT GATCAGGGAC AATGGAGTGA CGTGCCCTGC 
AACTACCACC TGTCCTACAC CTGCAAGATG GGGCTGGTGT CCTGTGGGCC GCCACCGGAG 
CTGCCCCTGG CTCAAGTGTT CGGCCGCCCA CGGCTGCGCT ATGAGGTGGA CACTGTGCTT 
CGCTACCGGT GCCGGGAAGG ACTGGCCCAG CGCAATCTGC CGCTGATCCG ATGCCAAGAG 
AACGGTCGTT GGGAGGCCCC CCAGATCTCC TGTGTGCCCA GAAGACCTGC CCGAGCTCTG 
CACCCAGAGG AGGACCCAGA AGGACGTCAG GGGAGGCTAC TGGGACGCTG GAAGGCGCTG 
TTGATCCCCC CTTCCAGCCC CATGCCAGGT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068767.3
CDS	337..3072
Translation	MAQLFLPLLAALVLAQAPAALADVLEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPAYPASLTDVSLALSELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVYCFRDSAQPSAIPEASNPASNPASDGLEAIVTVTETLEELQLPQEATESESRGAIYSIPIMEDGGGGSSTPEDPAEAPRTLLEFETQSMVPPTGFSEEEGKALEEEEKYEDEEEKEEEEEEEEVEDEALWAWPSELSSPGPEASLPTEPAAQEESLSQAPARAVLQPGASPLPDGESEASRPPRVHGPPTETLPTPRERNLASPSPSTLVEAREVGEATGGPELSGVPRGESEETGSSEGAPSLLPATRAPEGTRELEAPSEDNSGRTAPAGTSVQAQPVLPTDSASRGGVAVVPASGDCVPSPCHNGGTCLEEEEGVRCLCLPGYGGDLCDVGLRFCNPGWDAFQGACYKHFSTRRSWEEAETQCRMYGAHLASISTPEEQDFINNRYREYQWIGLNDRTIEGDFLWSDGVPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQWSDVPCNYHLSYTCKMGLVSCGPPPELPLAQVFGRPRLRYEVDTVLRYRCREGLAQRNLPLIRCQENGRWEAPQISCVPRRPARALHPEEDPEGRQGRLLGRWKALLIPPSSPMPGP

Target ORF information:

RefSeq Version	NM_021948.4
Organism	Homo sapiens(human)
Definition	Homo sapiens brevican (BCAN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021948.4

ORF Insert Sequence:

ATGGCCCAGC TGTTCCTGCC CCTGCTGGCA GCCCTGGTCC TGGCCCAGGC TCCTGCAGCT 
TTAGCAGATG TTCTGGAAGG AGACAGCTCA GAGGACCGCG CTTTTCGCGT GCGCATCGCG 
GGCGACGCGC CACTGCAGGG CGTGCTCGGC GGCGCCCTCA CCATCCCTTG CCACGTCCAC 
TACCTGCGGC CACCGCCGAG CCGCCGGGCT GTGCTGGGCT CTCCGCGGGT CAAGTGGACT 
TTCCTGTCCC GGGGCCGGGA GGCAGAGGTG CTGGTGGCGC GGGGAGTGCG CGTCAAGGTG 
AACGAGGCCT ACCGGTTCCG CGTGGCACTG CCTGCGTACC CAGCGTCGCT CACCGACGTC 
TCCCTGGCGC TGAGCGAGCT GCGCCCCAAC GACTCAGGTA TCTATCGCTG TGAGGTCCAG 
CACGGCATCG ATGACAGCAG CGACGCTGTG GAGGTCAAGG TCAAAGGGGT CGTCTTTCTC 
TACCGAGAGG GCTCTGCCCG CTATGCTTTC TCCTTTTCTG GGGCCCAGGA GGCCTGTGCC 
CGCATTGGAG CCCACATCGC CACCCCGGAG CAGCTCTATG CCGCCTACCT TGGGGGCTAT 
GAGCAATGTG ATGCTGGCTG GCTGTCGGAT CAGACCGTGA GGTATCCCAT CCAGACCCCA 
CGAGAGGCCT GTTACGGAGA CATGGATGGC TTCCCCGGGG TCCGGAACTA TGGTGTGGTG 
GACCCGGATG ACCTCTATGA TGTGTACTGT TATGCTGAAG ACCTAAATGG AGAACTGTTC 
CTGGGTGACC CTCCAGAGAA GCTGACATTG GAGGAAGCAC GGGCGTACTG CCAGGAGCGG 
GGTGCAGAGA TTGCCACCAC GGGCCAACTG TATGCAGCCT GGGATGGTGG CCTGGACCAC 
TGCAGCCCAG GGTGGCTAGC TGATGGCAGT GTGCGCTACC CCATCGTCAC ACCCAGCCAG 
CGCTGTGGTG GGGGCTTGCC TGGTGTCAAG ACTCTCTTCC TCTTCCCCAA CCAGACTGGC 
TTCCCCAATA AGCACAGCCG CTTCAACGTC TACTGCTTCC GAGACTCGGC CCAGCCTTCT 
GCCATCCCTG AGGCCTCCAA CCCAGCCTCC AACCCAGCCT CTGATGGACT AGAGGCTATC 
GTCACAGTGA CAGAGACCCT GGAGGAACTG CAGCTGCCTC AGGAAGCCAC AGAGAGTGAA 
TCCCGTGGGG CCATCTACTC CATCCCCATC ATGGAGGACG GAGGAGGTGG AAGCTCCACT 
CCAGAAGACC CAGCAGAGGC CCCTAGGACG CTCCTAGAAT TTGAAACACA ATCCATGGTA 
CCGCCCACGG GGTTCTCAGA AGAGGAAGGT AAGGCATTGG AGGAAGAAGA GAAATATGAA 
GATGAAGAAG AGAAAGAGGA GGAAGAAGAA GAGGAGGAGG TGGAGGATGA GGCTCTGTGG 
GCATGGCCCA GCGAGCTCAG CAGCCCGGGC CCTGAGGCCT CTCTCCCCAC TGAGCCAGCA 
GCCCAGGAGG AGTCACTCTC CCAGGCGCCA GCAAGGGCAG TCCTGCAGCC TGGTGCATCA 
CCACTTCCTG ATGGAGAGTC AGAAGCTTCC AGGCCTCCAA GGGTCCATGG ACCACCTACT 
GAGACTCTGC CCACTCCCAG GGAGAGGAAC CTAGCATCCC CATCACCTTC CACTCTGGTT 
GAGGCAAGAG AGGTGGGGGA GGCAACTGGT GGTCCTGAGC TATCTGGGGT CCCTCGAGGA 
GAGAGCGAGG AGACAGGAAG CTCCGAGGGT GCCCCTTCCC TGCTTCCAGC CACACGGGCC 
CCTGAGGGTA CCAGGGAGCT GGAGGCCCCC TCTGAAGATA ATTCTGGAAG AACTGCCCCA 
GCAGGGACCT CAGTGCAGGC CCAGCCAGTG CTGCCCACTG ACAGCGCCAG CCGAGGTGGA 
GTGGCCGTGG TCCCCGCATC AGGTGACTGT GTCCCCAGCC CCTGCCACAA TGGTGGGACA 
TGCTTGGAGG AGGAGGAAGG GGTCCGCTGC CTATGTCTGC CTGGCTATGG GGGGGACCTG 
TGCGATGTTG GCCTCCGCTT CTGCAACCCC GGCTGGGACG CCTTCCAGGG CGCCTGCTAC 
AAGCACTTTT CCACACGAAG GAGCTGGGAG GAGGCAGAGA CCCAGTGCCG GATGTACGGC 
GCGCATCTGG CCAGCATCAG CACACCCGAG GAACAGGACT TCATCAACAA CCGGTACCGG 
GAGTACCAGT GGATCGGACT CAACGACAGG ACCATCGAAG GCGACTTCTT GTGGTCGGAT 
GGCGTCCCCC TGCTCTATGA GAACTGGAAC CCTGGGCAGC CTGACAGCTA CTTCCTGTCT 
GGAGAGAACT GCGTGGTCAT GGTGTGGCAT GATCAGGGAC AATGGAGTGA CGTGCCCTGC 
AACTACCACC TGTCCTACAC CTGCAAGATG GGGCTGGTGT CCTGTGGGCC GCCACCGGAG 
CTGCCCCTGG CTCAAGTGTT CGGCCGCCCA CGGCTGCGCT ATGAGGTGGA CACTGTGCTT 
CGCTACCGGT GCCGGGAAGG ACTGGCCCAG CGCAATCTGC CGCTGATCCG ATGCCAAGAG 
AACGGTCGTT GGGAGGCCCC CCAGATCTCC TGTGTGCCCA GAAGACCTGC CCGAGCTCTG 
CACCCAGAGG AGGACCCAGA AGGACGTCAG GGGAGGCTAC TGGGACGCTG GAAGGCGCTG 
TTGATCCCCC CTTCCAGCCC CATGCCAGGT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAN ( XM_011509866.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508168.1 Homo sapiens brevican (BCAN), transcript variant X2, mRNA.

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CloneID	OHu22362
Clone ID Related Accession (Same CDS sequence)	NM_021948.5 , NM_021948.4 , XM_011509866.1
Accession Version	XM_011509866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	brevican core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC TGTTCCTGCC CCTGCTGGCA GCCCTGGTCC TGGCCCAGGC TCCTGCAGCT 
TTAGCAGATG TTCTGGAAGG AGACAGCTCA GAGGACCGCG CTTTTCGCGT GCGCATCGCG 
GGCGACGCGC CACTGCAGGG CGTGCTCGGC GGCGCCCTCA CCATCCCTTG CCACGTCCAC 
TACCTGCGGC CACCGCCGAG CCGCCGGGCT GTGCTGGGCT CTCCGCGGGT CAAGTGGACT 
TTCCTGTCCC GGGGCCGGGA GGCAGAGGTG CTGGTGGCGC GGGGAGTGCG CGTCAAGGTG 
AACGAGGCCT ACCGGTTCCG CGTGGCACTG CCTGCGTACC CAGCGTCGCT CACCGACGTC 
TCCCTGGCGC TGAGCGAGCT GCGCCCCAAC GACTCAGGTA TCTATCGCTG TGAGGTCCAG 
CACGGCATCG ATGACAGCAG CGACGCTGTG GAGGTCAAGG TCAAAGGGGT CGTCTTTCTC 
TACCGAGAGG GCTCTGCCCG CTATGCTTTC TCCTTTTCTG GGGCCCAGGA GGCCTGTGCC 
CGCATTGGAG CCCACATCGC CACCCCGGAG CAGCTCTATG CCGCCTACCT TGGGGGCTAT 
GAGCAATGTG ATGCTGGCTG GCTGTCGGAT CAGACCGTGA GGTATCCCAT CCAGACCCCA 
CGAGAGGCCT GTTACGGAGA CATGGATGGC TTCCCCGGGG TCCGGAACTA TGGTGTGGTG 
GACCCGGATG ACCTCTATGA TGTGTACTGT TATGCTGAAG ACCTAAATGG AGAACTGTTC 
CTGGGTGACC CTCCAGAGAA GCTGACATTG GAGGAAGCAC GGGCGTACTG CCAGGAGCGG 
GGTGCAGAGA TTGCCACCAC GGGCCAACTG TATGCAGCCT GGGATGGTGG CCTGGACCAC 
TGCAGCCCAG GGTGGCTAGC TGATGGCAGT GTGCGCTACC CCATCGTCAC ACCCAGCCAG 
CGCTGTGGTG GGGGCTTGCC TGGTGTCAAG ACTCTCTTCC TCTTCCCCAA CCAGACTGGC 
TTCCCCAATA AGCACAGCCG CTTCAACGTC TACTGCTTCC GAGACTCGGC CCAGCCTTCT 
GCCATCCCTG AGGCCTCCAA CCCAGCCTCC AACCCAGCCT CTGATGGACT AGAGGCTATC 
GTCACAGTGA CAGAGACCCT GGAGGAACTG CAGCTGCCTC AGGAAGCCAC AGAGAGTGAA 
TCCCGTGGGG CCATCTACTC CATCCCCATC ATGGAGGACG GAGGAGGTGG AAGCTCCACT 
CCAGAAGACC CAGCAGAGGC CCCTAGGACG CTCCTAGAAT TTGAAACACA ATCCATGGTA 
CCGCCCACGG GGTTCTCAGA AGAGGAAGGT AAGGCATTGG AGGAAGAAGA GAAATATGAA 
GATGAAGAAG AGAAAGAGGA GGAAGAAGAA GAGGAGGAGG TGGAGGATGA GGCTCTGTGG 
GCATGGCCCA GCGAGCTCAG CAGCCCGGGC CCTGAGGCCT CTCTCCCCAC TGAGCCAGCA 
GCCCAGGAGG AGTCACTCTC CCAGGCGCCA GCAAGGGCAG TCCTGCAGCC TGGTGCATCA 
CCACTTCCTG ATGGAGAGTC AGAAGCTTCC AGGCCTCCAA GGGTCCATGG ACCACCTACT 
GAGACTCTGC CCACTCCCAG GGAGAGGAAC CTAGCATCCC CATCACCTTC CACTCTGGTT 
GAGGCAAGAG AGGTGGGGGA GGCAACTGGT GGTCCTGAGC TATCTGGGGT CCCTCGAGGA 
GAGAGCGAGG AGACAGGAAG CTCCGAGGGT GCCCCTTCCC TGCTTCCAGC CACACGGGCC 
CCTGAGGGTA CCAGGGAGCT GGAGGCCCCC TCTGAAGATA ATTCTGGAAG AACTGCCCCA 
GCAGGGACCT CAGTGCAGGC CCAGCCAGTG CTGCCCACTG ACAGCGCCAG CCGAGGTGGA 
GTGGCCGTGG TCCCCGCATC AGGTGACTGT GTCCCCAGCC CCTGCCACAA TGGTGGGACA 
TGCTTGGAGG AGGAGGAAGG GGTCCGCTGC CTATGTCTGC CTGGCTATGG GGGGGACCTG 
TGCGATGTTG GCCTCCGCTT CTGCAACCCC GGCTGGGACG CCTTCCAGGG CGCCTGCTAC 
AAGCACTTTT CCACACGAAG GAGCTGGGAG GAGGCAGAGA CCCAGTGCCG GATGTACGGC 
GCGCATCTGG CCAGCATCAG CACACCCGAG GAACAGGACT TCATCAACAA CCGGTACCGG 
GAGTACCAGT GGATCGGACT CAACGACAGG ACCATCGAAG GCGACTTCTT GTGGTCGGAT 
GGCGTCCCCC TGCTCTATGA GAACTGGAAC CCTGGGCAGC CTGACAGCTA CTTCCTGTCT 
GGAGAGAACT GCGTGGTCAT GGTGTGGCAT GATCAGGGAC AATGGAGTGA CGTGCCCTGC 
AACTACCACC TGTCCTACAC CTGCAAGATG GGGCTGGTGT CCTGTGGGCC GCCACCGGAG 
CTGCCCCTGG CTCAAGTGTT CGGCCGCCCA CGGCTGCGCT ATGAGGTGGA CACTGTGCTT 
CGCTACCGGT GCCGGGAAGG ACTGGCCCAG CGCAATCTGC CGCTGATCCG ATGCCAAGAG 
AACGGTCGTT GGGAGGCCCC CCAGATCTCC TGTGTGCCCA GAAGACCTGC CCGAGCTCTG 
CACCCAGAGG AGGACCCAGA AGGACGTCAG GGGAGGCTAC TGGGACGCTG GAAGGCGCTG 
TTGATCCCCC CTTCCAGCCC CATGCCAGGT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508168.1
CDS	164..2899
Translation	MAQLFLPLLAALVLAQAPAALADVLEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPAYPASLTDVSLALSELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVYCFRDSAQPSAIPEASNPASNPASDGLEAIVTVTETLEELQLPQEATESESRGAIYSIPIMEDGGGGSSTPEDPAEAPRTLLEFETQSMVPPTGFSEEEGKALEEEEKYEDEEEKEEEEEEEEVEDEALWAWPSELSSPGPEASLPTEPAAQEESLSQAPARAVLQPGASPLPDGESEASRPPRVHGPPTETLPTPRERNLASPSPSTLVEAREVGEATGGPELSGVPRGESEETGSSEGAPSLLPATRAPEGTRELEAPSEDNSGRTAPAGTSVQAQPVLPTDSASRGGVAVVPASGDCVPSPCHNGGTCLEEEEGVRCLCLPGYGGDLCDVGLRFCNPGWDAFQGACYKHFSTRRSWEEAETQCRMYGAHLASISTPEEQDFINNRYREYQWIGLNDRTIEGDFLWSDGVPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQWSDVPCNYHLSYTCKMGLVSCGPPPELPLAQVFGRPRLRYEVDTVLRYRCREGLAQRNLPLIRCQENGRWEAPQISCVPRRPARALHPEEDPEGRQGRLLGRWKALLIPPSSPMPGP

Target ORF information:

RefSeq Version	XM_011509866.1
Organism	Homo sapiens(human)
Definition	Homo sapiens brevican (BCAN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509866.1

ORF Insert Sequence:

ATGGCCCAGC TGTTCCTGCC CCTGCTGGCA GCCCTGGTCC TGGCCCAGGC TCCTGCAGCT 
TTAGCAGATG TTCTGGAAGG AGACAGCTCA GAGGACCGCG CTTTTCGCGT GCGCATCGCG 
GGCGACGCGC CACTGCAGGG CGTGCTCGGC GGCGCCCTCA CCATCCCTTG CCACGTCCAC 
TACCTGCGGC CACCGCCGAG CCGCCGGGCT GTGCTGGGCT CTCCGCGGGT CAAGTGGACT 
TTCCTGTCCC GGGGCCGGGA GGCAGAGGTG CTGGTGGCGC GGGGAGTGCG CGTCAAGGTG 
AACGAGGCCT ACCGGTTCCG CGTGGCACTG CCTGCGTACC CAGCGTCGCT CACCGACGTC 
TCCCTGGCGC TGAGCGAGCT GCGCCCCAAC GACTCAGGTA TCTATCGCTG TGAGGTCCAG 
CACGGCATCG ATGACAGCAG CGACGCTGTG GAGGTCAAGG TCAAAGGGGT CGTCTTTCTC 
TACCGAGAGG GCTCTGCCCG CTATGCTTTC TCCTTTTCTG GGGCCCAGGA GGCCTGTGCC 
CGCATTGGAG CCCACATCGC CACCCCGGAG CAGCTCTATG CCGCCTACCT TGGGGGCTAT 
GAGCAATGTG ATGCTGGCTG GCTGTCGGAT CAGACCGTGA GGTATCCCAT CCAGACCCCA 
CGAGAGGCCT GTTACGGAGA CATGGATGGC TTCCCCGGGG TCCGGAACTA TGGTGTGGTG 
GACCCGGATG ACCTCTATGA TGTGTACTGT TATGCTGAAG ACCTAAATGG AGAACTGTTC 
CTGGGTGACC CTCCAGAGAA GCTGACATTG GAGGAAGCAC GGGCGTACTG CCAGGAGCGG 
GGTGCAGAGA TTGCCACCAC GGGCCAACTG TATGCAGCCT GGGATGGTGG CCTGGACCAC 
TGCAGCCCAG GGTGGCTAGC TGATGGCAGT GTGCGCTACC CCATCGTCAC ACCCAGCCAG 
CGCTGTGGTG GGGGCTTGCC TGGTGTCAAG ACTCTCTTCC TCTTCCCCAA CCAGACTGGC 
TTCCCCAATA AGCACAGCCG CTTCAACGTC TACTGCTTCC GAGACTCGGC CCAGCCTTCT 
GCCATCCCTG AGGCCTCCAA CCCAGCCTCC AACCCAGCCT CTGATGGACT AGAGGCTATC 
GTCACAGTGA CAGAGACCCT GGAGGAACTG CAGCTGCCTC AGGAAGCCAC AGAGAGTGAA 
TCCCGTGGGG CCATCTACTC CATCCCCATC ATGGAGGACG GAGGAGGTGG AAGCTCCACT 
CCAGAAGACC CAGCAGAGGC CCCTAGGACG CTCCTAGAAT TTGAAACACA ATCCATGGTA 
CCGCCCACGG GGTTCTCAGA AGAGGAAGGT AAGGCATTGG AGGAAGAAGA GAAATATGAA 
GATGAAGAAG AGAAAGAGGA GGAAGAAGAA GAGGAGGAGG TGGAGGATGA GGCTCTGTGG 
GCATGGCCCA GCGAGCTCAG CAGCCCGGGC CCTGAGGCCT CTCTCCCCAC TGAGCCAGCA 
GCCCAGGAGG AGTCACTCTC CCAGGCGCCA GCAAGGGCAG TCCTGCAGCC TGGTGCATCA 
CCACTTCCTG ATGGAGAGTC AGAAGCTTCC AGGCCTCCAA GGGTCCATGG ACCACCTACT 
GAGACTCTGC CCACTCCCAG GGAGAGGAAC CTAGCATCCC CATCACCTTC CACTCTGGTT 
GAGGCAAGAG AGGTGGGGGA GGCAACTGGT GGTCCTGAGC TATCTGGGGT CCCTCGAGGA 
GAGAGCGAGG AGACAGGAAG CTCCGAGGGT GCCCCTTCCC TGCTTCCAGC CACACGGGCC 
CCTGAGGGTA CCAGGGAGCT GGAGGCCCCC TCTGAAGATA ATTCTGGAAG AACTGCCCCA 
GCAGGGACCT CAGTGCAGGC CCAGCCAGTG CTGCCCACTG ACAGCGCCAG CCGAGGTGGA 
GTGGCCGTGG TCCCCGCATC AGGTGACTGT GTCCCCAGCC CCTGCCACAA TGGTGGGACA 
TGCTTGGAGG AGGAGGAAGG GGTCCGCTGC CTATGTCTGC CTGGCTATGG GGGGGACCTG 
TGCGATGTTG GCCTCCGCTT CTGCAACCCC GGCTGGGACG CCTTCCAGGG CGCCTGCTAC 
AAGCACTTTT CCACACGAAG GAGCTGGGAG GAGGCAGAGA CCCAGTGCCG GATGTACGGC 
GCGCATCTGG CCAGCATCAG CACACCCGAG GAACAGGACT TCATCAACAA CCGGTACCGG 
GAGTACCAGT GGATCGGACT CAACGACAGG ACCATCGAAG GCGACTTCTT GTGGTCGGAT 
GGCGTCCCCC TGCTCTATGA GAACTGGAAC CCTGGGCAGC CTGACAGCTA CTTCCTGTCT 
GGAGAGAACT GCGTGGTCAT GGTGTGGCAT GATCAGGGAC AATGGAGTGA CGTGCCCTGC 
AACTACCACC TGTCCTACAC CTGCAAGATG GGGCTGGTGT CCTGTGGGCC GCCACCGGAG 
CTGCCCCTGG CTCAAGTGTT CGGCCGCCCA CGGCTGCGCT ATGAGGTGGA CACTGTGCTT 
CGCTACCGGT GCCGGGAAGG ACTGGCCCAG CGCAATCTGC CGCTGATCCG ATGCCAAGAG 
AACGGTCGTT GGGAGGCCCC CCAGATCTCC TGTGTGCCCA GAAGACCTGC CCGAGCTCTG 
CACCCAGAGG AGGACCCAGA AGGACGTCAG GGGAGGCTAC TGGGACGCTG GAAGGCGCTG 
TTGATCCCCC CTTCCAGCCC CATGCCAGGT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAN ( NM_198427.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_940819.1 Homo sapiens brevican (BCAN), transcript variant 2, mRNA.

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CloneID	OHu29330
Clone ID Related Accession (Same CDS sequence)	NM_198427.1 , NM_198427.2
Accession Version	NM_198427.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	brevican core protein isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY358372.1, AF229053.1 and AW298823.1. Transcript Variant: This variant (2) differs in the 3' UTR, and coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.118256.1, SRR1803612.112092.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGC TGTTCCTGCC CCTGCTGGCA GCCCTGGTCC TGGCCCAGGC TCCTGCAGCT 
TTAGCAGATG TTCTGGAAGG AGACAGCTCA GAGGACCGCG CTTTTCGCGT GCGCATCGCG 
GGCGACGCGC CACTGCAGGG CGTGCTCGGC GGCGCCCTCA CCATCCCTTG CCACGTCCAC 
TACCTGCGGC CACCGCCGAG CCGCCGGGCT GTGCTGGGCT CTCCGCGGGT CAAGTGGACT 
TTCCTGTCCC GGGGCCGGGA GGCAGAGGTG CTGGTGGCGC GGGGAGTGCG CGTCAAGGTG 
AACGAGGCCT ACCGGTTCCG CGTGGCACTG CCTGCGTACC CAGCGTCGCT CACCGACGTC 
TCCCTGGCGC TGAGCGAGCT GCGCCCCAAC GACTCAGGTA TCTATCGCTG TGAGGTCCAG 
CACGGCATCG ATGACAGCAG CGACGCTGTG GAGGTCAAGG TCAAAGGGGT CGTCTTTCTC 
TACCGAGAGG GCTCTGCCCG CTATGCTTTC TCCTTTTCTG GGGCCCAGGA GGCCTGTGCC 
CGCATTGGAG CCCACATCGC CACCCCGGAG CAGCTCTATG CCGCCTACCT TGGGGGCTAT 
GAGCAATGTG ATGCTGGCTG GCTGTCGGAT CAGACCGTGA GGTATCCCAT CCAGACCCCA 
CGAGAGGCCT GTTACGGAGA CATGGATGGC TTCCCCGGGG TCCGGAACTA TGGTGTGGTG 
GACCCGGATG ACCTCTATGA TGTGTACTGT TATGCTGAAG ACCTAAATGG AGAACTGTTC 
CTGGGTGACC CTCCAGAGAA GCTGACATTG GAGGAAGCAC GGGCGTACTG CCAGGAGCGG 
GGTGCAGAGA TTGCCACCAC GGGCCAACTG TATGCAGCCT GGGATGGTGG CCTGGACCAC 
TGCAGCCCAG GGTGGCTAGC TGATGGCAGT GTGCGCTACC CCATCGTCAC ACCCAGCCAG 
CGCTGTGGTG GGGGCTTGCC TGGTGTCAAG ACTCTCTTCC TCTTCCCCAA CCAGACTGGC 
TTCCCCAATA AGCACAGCCG CTTCAACGTC TACTGCTTCC GAGACTCGGC CCAGCCTTCT 
GCCATCCCTG AGGCCTCCAA CCCAGCCTCC AACCCAGCCT CTGATGGACT AGAGGCTATC 
GTCACAGTGA CAGAGACCCT GGAGGAACTG CAGCTGCCTC AGGAAGCCAC AGAGAGTGAA 
TCCCGTGGGG CCATCTACTC CATCCCCATC ATGGAGGACG GAGGAGGTGG AAGCTCCACT 
CCAGAAGACC CAGCAGAGGC CCCTAGGACG CTCCTAGAAT TTGAAACACA ATCCATGGTA 
CCGCCCACGG GGTTCTCAGA AGAGGAAGGT AAGGCATTGG AGGAAGAAGA GAAATATGAA 
GATGAAGAAG AGAAAGAGGA GGAAGAAGAA GAGGAGGAGG TGGAGGATGA GGCTCTGTGG 
GCATGGCCCA GCGAGCTCAG CAGCCCGGGC CCTGAGGCCT CTCTCCCCAC TGAGCCAGCA 
GCCCAGGAGG AGTCACTCTC CCAGGCGCCA GCAAGGGCAG TCCTGCAGCC TGGTGCATCA 
CCACTTCCTG ATGGAGAGTC AGAAGCTTCC AGGCCTCCAA GGGTCCATGG ACCACCTACT 
GAGACTCTGC CCACTCCCAG GGAGAGGAAC CTAGCATCCC CATCACCTTC CACTCTGGTT 
GAGGCAAGAG AGGTGGGGGA GGCAACTGGT GGTCCTGAGC TATCTGGGGT CCCTCGAGGA 
GAGAGCGAGG AGACAGGAAG CTCCGAGGGT GCCCCTTCCC TGCTTCCAGC CACACGGGCC 
CCTGAGGGTA CCAGGGAGCT GGAGGCCCCC TCTGAAGATA ATTCTGGAAG AACTGCCCCA 
GCAGGGACCT CAGTGCAGGC CCAGCCAGTG CTGCCCACTG ACAGCGCCAG CCGAGGTGGA 
GTGGCCGTGG TCCCCGCATC AGGTAATTCT GCCCAAGGCT CAACTGCCCT CTCTATCCTA 
CTCCTTTTCT TCCCCCTGCA GCTCTGGGTC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_940819.1
CDS	337..2352
Translation	MAQLFLPLLAALVLAQAPAALADVLEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPAYPASLTDVSLALSELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVYCFRDSAQPSAIPEASNPASNPASDGLEAIVTVTETLEELQLPQEATESESRGAIYSIPIMEDGGGGSSTPEDPAEAPRTLLEFETQSMVPPTGFSEEEGKALEEEEKYEDEEEKEEEEEEEEVEDEALWAWPSELSSPGPEASLPTEPAAQEESLSQAPARAVLQPGASPLPDGESEASRPPRVHGPPTETLPTPRERNLASPSPSTLVEAREVGEATGGPELSGVPRGESEETGSSEGAPSLLPATRAPEGTRELEAPSEDNSGRTAPAGTSVQAQPVLPTDSASRGGVAVVPASGNSAQGSTALSILLLFFPLQLWVT

Target ORF information:

RefSeq Version	NM_198427.1
Organism	Homo sapiens(human)
Definition	Homo sapiens brevican (BCAN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198427.1

ORF Insert Sequence:

ATGGCCCAGC TGTTCCTGCC CCTGCTGGCA GCCCTGGTCC TGGCCCAGGC TCCTGCAGCT 
TTAGCAGATG TTCTGGAAGG AGACAGCTCA GAGGACCGCG CTTTTCGCGT GCGCATCGCG 
GGCGACGCGC CACTGCAGGG CGTGCTCGGC GGCGCCCTCA CCATCCCTTG CCACGTCCAC 
TACCTGCGGC CACCGCCGAG CCGCCGGGCT GTGCTGGGCT CTCCGCGGGT CAAGTGGACT 
TTCCTGTCCC GGGGCCGGGA GGCAGAGGTG CTGGTGGCGC GGGGAGTGCG CGTCAAGGTG 
AACGAGGCCT ACCGGTTCCG CGTGGCACTG CCTGCGTACC CAGCGTCGCT CACCGACGTC 
TCCCTGGCGC TGAGCGAGCT GCGCCCCAAC GACTCAGGTA TCTATCGCTG TGAGGTCCAG 
CACGGCATCG ATGACAGCAG CGACGCTGTG GAGGTCAAGG TCAAAGGGGT CGTCTTTCTC 
TACCGAGAGG GCTCTGCCCG CTATGCTTTC TCCTTTTCTG GGGCCCAGGA GGCCTGTGCC 
CGCATTGGAG CCCACATCGC CACCCCGGAG CAGCTCTATG CCGCCTACCT TGGGGGCTAT 
GAGCAATGTG ATGCTGGCTG GCTGTCGGAT CAGACCGTGA GGTATCCCAT CCAGACCCCA 
CGAGAGGCCT GTTACGGAGA CATGGATGGC TTCCCCGGGG TCCGGAACTA TGGTGTGGTG 
GACCCGGATG ACCTCTATGA TGTGTACTGT TATGCTGAAG ACCTAAATGG AGAACTGTTC 
CTGGGTGACC CTCCAGAGAA GCTGACATTG GAGGAAGCAC GGGCGTACTG CCAGGAGCGG 
GGTGCAGAGA TTGCCACCAC GGGCCAACTG TATGCAGCCT GGGATGGTGG CCTGGACCAC 
TGCAGCCCAG GGTGGCTAGC TGATGGCAGT GTGCGCTACC CCATCGTCAC ACCCAGCCAG 
CGCTGTGGTG GGGGCTTGCC TGGTGTCAAG ACTCTCTTCC TCTTCCCCAA CCAGACTGGC 
TTCCCCAATA AGCACAGCCG CTTCAACGTC TACTGCTTCC GAGACTCGGC CCAGCCTTCT 
GCCATCCCTG AGGCCTCCAA CCCAGCCTCC AACCCAGCCT CTGATGGACT AGAGGCTATC 
GTCACAGTGA CAGAGACCCT GGAGGAACTG CAGCTGCCTC AGGAAGCCAC AGAGAGTGAA 
TCCCGTGGGG CCATCTACTC CATCCCCATC ATGGAGGACG GAGGAGGTGG AAGCTCCACT 
CCAGAAGACC CAGCAGAGGC CCCTAGGACG CTCCTAGAAT TTGAAACACA ATCCATGGTA 
CCGCCCACGG GGTTCTCAGA AGAGGAAGGT AAGGCATTGG AGGAAGAAGA GAAATATGAA 
GATGAAGAAG AGAAAGAGGA GGAAGAAGAA GAGGAGGAGG TGGAGGATGA GGCTCTGTGG 
GCATGGCCCA GCGAGCTCAG CAGCCCGGGC CCTGAGGCCT CTCTCCCCAC TGAGCCAGCA 
GCCCAGGAGG AGTCACTCTC CCAGGCGCCA GCAAGGGCAG TCCTGCAGCC TGGTGCATCA 
CCACTTCCTG ATGGAGAGTC AGAAGCTTCC AGGCCTCCAA GGGTCCATGG ACCACCTACT 
GAGACTCTGC CCACTCCCAG GGAGAGGAAC CTAGCATCCC CATCACCTTC CACTCTGGTT 
GAGGCAAGAG AGGTGGGGGA GGCAACTGGT GGTCCTGAGC TATCTGGGGT CCCTCGAGGA 
GAGAGCGAGG AGACAGGAAG CTCCGAGGGT GCCCCTTCCC TGCTTCCAGC CACACGGGCC 
CCTGAGGGTA CCAGGGAGCT GGAGGCCCCC TCTGAAGATA ATTCTGGAAG AACTGCCCCA 
GCAGGGACCT CAGTGCAGGC CCAGCCAGTG CTGCCCACTG ACAGCGCCAG CCGAGGTGGA 
GTGGCCGTGG TCCCCGCATC AGGTAATTCT GCCCAAGGCT CAACTGCCCT CTCTATCCTA 
CTCCTTTTCT TCCCCCTGCA GCTCTGGGTC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAN ( NM_198427.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_940819.1 Homo sapiens brevican (BCAN), transcript variant 2, mRNA.

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CloneID	OHu29330
Clone ID Related Accession (Same CDS sequence)	NM_198427.1 , NM_198427.2
Accession Version	NM_198427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	brevican core protein isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY358372.1, AF229053.1 and AW298823.1. On May 31, 2019 this sequence version replaced NM_198427.1. Transcript Variant: This variant (2) differs in the 3' UTR, and coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.900725.1, SRR1660803.222314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGC TGTTCCTGCC CCTGCTGGCA GCCCTGGTCC TGGCCCAGGC TCCTGCAGCT 
TTAGCAGATG TTCTGGAAGG AGACAGCTCA GAGGACCGCG CTTTTCGCGT GCGCATCGCG 
GGCGACGCGC CACTGCAGGG CGTGCTCGGC GGCGCCCTCA CCATCCCTTG CCACGTCCAC 
TACCTGCGGC CACCGCCGAG CCGCCGGGCT GTGCTGGGCT CTCCGCGGGT CAAGTGGACT 
TTCCTGTCCC GGGGCCGGGA GGCAGAGGTG CTGGTGGCGC GGGGAGTGCG CGTCAAGGTG 
AACGAGGCCT ACCGGTTCCG CGTGGCACTG CCTGCGTACC CAGCGTCGCT CACCGACGTC 
TCCCTGGCGC TGAGCGAGCT GCGCCCCAAC GACTCAGGTA TCTATCGCTG TGAGGTCCAG 
CACGGCATCG ATGACAGCAG CGACGCTGTG GAGGTCAAGG TCAAAGGGGT CGTCTTTCTC 
TACCGAGAGG GCTCTGCCCG CTATGCTTTC TCCTTTTCTG GGGCCCAGGA GGCCTGTGCC 
CGCATTGGAG CCCACATCGC CACCCCGGAG CAGCTCTATG CCGCCTACCT TGGGGGCTAT 
GAGCAATGTG ATGCTGGCTG GCTGTCGGAT CAGACCGTGA GGTATCCCAT CCAGACCCCA 
CGAGAGGCCT GTTACGGAGA CATGGATGGC TTCCCCGGGG TCCGGAACTA TGGTGTGGTG 
GACCCGGATG ACCTCTATGA TGTGTACTGT TATGCTGAAG ACCTAAATGG AGAACTGTTC 
CTGGGTGACC CTCCAGAGAA GCTGACATTG GAGGAAGCAC GGGCGTACTG CCAGGAGCGG 
GGTGCAGAGA TTGCCACCAC GGGCCAACTG TATGCAGCCT GGGATGGTGG CCTGGACCAC 
TGCAGCCCAG GGTGGCTAGC TGATGGCAGT GTGCGCTACC CCATCGTCAC ACCCAGCCAG 
CGCTGTGGTG GGGGCTTGCC TGGTGTCAAG ACTCTCTTCC TCTTCCCCAA CCAGACTGGC 
TTCCCCAATA AGCACAGCCG CTTCAACGTC TACTGCTTCC GAGACTCGGC CCAGCCTTCT 
GCCATCCCTG AGGCCTCCAA CCCAGCCTCC AACCCAGCCT CTGATGGACT AGAGGCTATC 
GTCACAGTGA CAGAGACCCT GGAGGAACTG CAGCTGCCTC AGGAAGCCAC AGAGAGTGAA 
TCCCGTGGGG CCATCTACTC CATCCCCATC ATGGAGGACG GAGGAGGTGG AAGCTCCACT 
CCAGAAGACC CAGCAGAGGC CCCTAGGACG CTCCTAGAAT TTGAAACACA ATCCATGGTA 
CCGCCCACGG GGTTCTCAGA AGAGGAAGGT AAGGCATTGG AGGAAGAAGA GAAATATGAA 
GATGAAGAAG AGAAAGAGGA GGAAGAAGAA GAGGAGGAGG TGGAGGATGA GGCTCTGTGG 
GCATGGCCCA GCGAGCTCAG CAGCCCGGGC CCTGAGGCCT CTCTCCCCAC TGAGCCAGCA 
GCCCAGGAGG AGTCACTCTC CCAGGCGCCA GCAAGGGCAG TCCTGCAGCC TGGTGCATCA 
CCACTTCCTG ATGGAGAGTC AGAAGCTTCC AGGCCTCCAA GGGTCCATGG ACCACCTACT 
GAGACTCTGC CCACTCCCAG GGAGAGGAAC CTAGCATCCC CATCACCTTC CACTCTGGTT 
GAGGCAAGAG AGGTGGGGGA GGCAACTGGT GGTCCTGAGC TATCTGGGGT CCCTCGAGGA 
GAGAGCGAGG AGACAGGAAG CTCCGAGGGT GCCCCTTCCC TGCTTCCAGC CACACGGGCC 
CCTGAGGGTA CCAGGGAGCT GGAGGCCCCC TCTGAAGATA ATTCTGGAAG AACTGCCCCA 
GCAGGGACCT CAGTGCAGGC CCAGCCAGTG CTGCCCACTG ACAGCGCCAG CCGAGGTGGA 
GTGGCCGTGG TCCCCGCATC AGGTAATTCT GCCCAAGGCT CAACTGCCCT CTCTATCCTA 
CTCCTTTTCT TCCCCCTGCA GCTCTGGGTC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_940819.1
CDS	168..2183
Translation	MAQLFLPLLAALVLAQAPAALADVLEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPAYPASLTDVSLALSELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVYCFRDSAQPSAIPEASNPASNPASDGLEAIVTVTETLEELQLPQEATESESRGAIYSIPIMEDGGGGSSTPEDPAEAPRTLLEFETQSMVPPTGFSEEEGKALEEEEKYEDEEEKEEEEEEEEVEDEALWAWPSELSSPGPEASLPTEPAAQEESLSQAPARAVLQPGASPLPDGESEASRPPRVHGPPTETLPTPRERNLASPSPSTLVEAREVGEATGGPELSGVPRGESEETGSSEGAPSLLPATRAPEGTRELEAPSEDNSGRTAPAGTSVQAQPVLPTDSASRGGVAVVPASGNSAQGSTALSILLLFFPLQLWVT

Target ORF information:

RefSeq Version	NM_198427.2
Organism	Homo sapiens(human)
Definition	Homo sapiens brevican (BCAN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198427.2

ORF Insert Sequence:

ATGGCCCAGC TGTTCCTGCC CCTGCTGGCA GCCCTGGTCC TGGCCCAGGC TCCTGCAGCT 
TTAGCAGATG TTCTGGAAGG AGACAGCTCA GAGGACCGCG CTTTTCGCGT GCGCATCGCG 
GGCGACGCGC CACTGCAGGG CGTGCTCGGC GGCGCCCTCA CCATCCCTTG CCACGTCCAC 
TACCTGCGGC CACCGCCGAG CCGCCGGGCT GTGCTGGGCT CTCCGCGGGT CAAGTGGACT 
TTCCTGTCCC GGGGCCGGGA GGCAGAGGTG CTGGTGGCGC GGGGAGTGCG CGTCAAGGTG 
AACGAGGCCT ACCGGTTCCG CGTGGCACTG CCTGCGTACC CAGCGTCGCT CACCGACGTC 
TCCCTGGCGC TGAGCGAGCT GCGCCCCAAC GACTCAGGTA TCTATCGCTG TGAGGTCCAG 
CACGGCATCG ATGACAGCAG CGACGCTGTG GAGGTCAAGG TCAAAGGGGT CGTCTTTCTC 
TACCGAGAGG GCTCTGCCCG CTATGCTTTC TCCTTTTCTG GGGCCCAGGA GGCCTGTGCC 
CGCATTGGAG CCCACATCGC CACCCCGGAG CAGCTCTATG CCGCCTACCT TGGGGGCTAT 
GAGCAATGTG ATGCTGGCTG GCTGTCGGAT CAGACCGTGA GGTATCCCAT CCAGACCCCA 
CGAGAGGCCT GTTACGGAGA CATGGATGGC TTCCCCGGGG TCCGGAACTA TGGTGTGGTG 
GACCCGGATG ACCTCTATGA TGTGTACTGT TATGCTGAAG ACCTAAATGG AGAACTGTTC 
CTGGGTGACC CTCCAGAGAA GCTGACATTG GAGGAAGCAC GGGCGTACTG CCAGGAGCGG 
GGTGCAGAGA TTGCCACCAC GGGCCAACTG TATGCAGCCT GGGATGGTGG CCTGGACCAC 
TGCAGCCCAG GGTGGCTAGC TGATGGCAGT GTGCGCTACC CCATCGTCAC ACCCAGCCAG 
CGCTGTGGTG GGGGCTTGCC TGGTGTCAAG ACTCTCTTCC TCTTCCCCAA CCAGACTGGC 
TTCCCCAATA AGCACAGCCG CTTCAACGTC TACTGCTTCC GAGACTCGGC CCAGCCTTCT 
GCCATCCCTG AGGCCTCCAA CCCAGCCTCC AACCCAGCCT CTGATGGACT AGAGGCTATC 
GTCACAGTGA CAGAGACCCT GGAGGAACTG CAGCTGCCTC AGGAAGCCAC AGAGAGTGAA 
TCCCGTGGGG CCATCTACTC CATCCCCATC ATGGAGGACG GAGGAGGTGG AAGCTCCACT 
CCAGAAGACC CAGCAGAGGC CCCTAGGACG CTCCTAGAAT TTGAAACACA ATCCATGGTA 
CCGCCCACGG GGTTCTCAGA AGAGGAAGGT AAGGCATTGG AGGAAGAAGA GAAATATGAA 
GATGAAGAAG AGAAAGAGGA GGAAGAAGAA GAGGAGGAGG TGGAGGATGA GGCTCTGTGG 
GCATGGCCCA GCGAGCTCAG CAGCCCGGGC CCTGAGGCCT CTCTCCCCAC TGAGCCAGCA 
GCCCAGGAGG AGTCACTCTC CCAGGCGCCA GCAAGGGCAG TCCTGCAGCC TGGTGCATCA 
CCACTTCCTG ATGGAGAGTC AGAAGCTTCC AGGCCTCCAA GGGTCCATGG ACCACCTACT 
GAGACTCTGC CCACTCCCAG GGAGAGGAAC CTAGCATCCC CATCACCTTC CACTCTGGTT 
GAGGCAAGAG AGGTGGGGGA GGCAACTGGT GGTCCTGAGC TATCTGGGGT CCCTCGAGGA 
GAGAGCGAGG AGACAGGAAG CTCCGAGGGT GCCCCTTCCC TGCTTCCAGC CACACGGGCC 
CCTGAGGGTA CCAGGGAGCT GGAGGCCCCC TCTGAAGATA ATTCTGGAAG AACTGCCCCA 
GCAGGGACCT CAGTGCAGGC CCAGCCAGTG CTGCCCACTG ACAGCGCCAG CCGAGGTGGA 
GTGGCCGTGG TCCCCGCATC AGGTAATTCT GCCCAAGGCT CAACTGCCCT CTCTATCCTA 
CTCCTTTTCT TCCCCCTGCA GCTCTGGGTC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAN ( XM_017002047.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857536.1 Homo sapiens brevican (BCAN), transcript variant X1, mRNA.

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CloneID	OHu90378
Clone ID Related Accession (Same CDS sequence)	XM_017002047.1
Accession Version	XM_017002047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	brevican core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGGA GTAAAGAGAG GAGTTTCCTT ACAAATAATG GTGGGGAGGA AGTAGAGGAG 
AGGGTGGGCT CAGGAGAAGC TGTGCTTACT TGCGTTCGGT CTAGGAGGCC CTCCTGGAAA 
AGGCCAGCCT GCAGCATGGC CCAGCTGTTC CTGCCCCTGC TGGCAGCCCT GGTCCTGGCC 
CAGGCTCCTG CAGCTTTAGC AGATGTTCTG GAAGGAGACA GCTCAGAGGA CCGCGCTTTT 
CGCGTGCGCA TCGCGGGCGA CGCGCCACTG CAGGGCGTGC TCGGCGGCGC CCTCACCATC 
CCTTGCCACG TCCACTACCT GCGGCCACCG CCGAGCCGCC GGGCTGTGCT GGGCTCTCCG 
CGGGTCAAGT GGACTTTCCT GTCCCGGGGC CGGGAGGCAG AGGTGCTGGT GGCGCGGGGA 
GTGCGCGTCA AGGTGAACGA GGCCTACCGG TTCCGCGTGG CACTGCCTGC GTACCCAGCG 
TCGCTCACCG ACGTCTCCCT GGCGCTGAGC GAGCTGCGCC CCAACGACTC AGGTATCTAT 
CGCTGTGAGG TCCAGCACGG CATCGATGAC AGCAGCGACG CTGTGGAGGT CAAGGTCAAA 
GGGGTCGTCT TTCTCTACCG AGAGGGCTCT GCCCGCTATG CTTTCTCCTT TTCTGGGGCC 
CAGGAGGCCT GTGCCCGCAT TGGAGCCCAC ATCGCCACCC CGGAGCAGCT CTATGCCGCC 
TACCTTGGGG GCTATGAGCA ATGTGATGCT GGCTGGCTGT CGGATCAGAC CGTGAGGTAT 
CCCATCCAGA CCCCACGAGA GGCCTGTTAC GGAGACATGG ATGGCTTCCC CGGGGTCCGG 
AACTATGGTG TGGTGGACCC GGATGACCTC TATGATGTGT ACTGTTATGC TGAAGACCTA 
AATGGAGAAC TGTTCCTGGG TGACCCTCCA GAGAAGCTGA CATTGGAGGA AGCACGGGCG 
TACTGCCAGG AGCGGGGTGC AGAGATTGCC ACCACGGGCC AACTGTATGC AGCCTGGGAT 
GGTGGCCTGG ACCACTGCAG CCCAGGGTGG CTAGCTGATG GCAGTGTGCG CTACCCCATC 
GTCACACCCA GCCAGCGCTG TGGTGGGGGC TTGCCTGGTG TCAAGACTCT CTTCCTCTTC 
CCCAACCAGA CTGGCTTCCC CAATAAGCAC AGCCGCTTCA ACGTCTACTG CTTCCGAGAC 
TCGGCCCAGC CTTCTGCCAT CCCTGAGGCC TCCAACCCAG CCTCCAACCC AGCCTCTGAT 
GGACTAGAGG CTATCGTCAC AGTGACAGAG ACCCTGGAGG AACTGCAGCT GCCTCAGGAA 
GCCACAGAGA GTGAATCCCG TGGGGCCATC TACTCCATCC CCATCATGGA GGACGGAGGA 
GGTGGAAGCT CCACTCCAGA AGACCCAGCA GAGGCCCCTA GGACGCTCCT AGAATTTGAA 
ACACAATCCA TGGTACCGCC CACGGGGTTC TCAGAAGAGG AAGGTAAGGC ATTGGAGGAA 
GAAGAGAAAT ATGAAGATGA AGAAGAGAAA GAGGAGGAAG AAGAAGAGGA GGAGGTGGAG 
GATGAGGCTC TGTGGGCATG GCCCAGCGAG CTCAGCAGCC CGGGCCCTGA GGCCTCTCTC 
CCCACTGAGC CAGCAGCCCA GGAGGAGTCA CTCTCCCAGG CGCCAGCAAG GGCAGTCCTG 
CAGCCTGGTG CATCACCACT TCCTGATGGA GAGTCAGAAG CTTCCAGGCC TCCAAGGGTC 
CATGGACCAC CTACTGAGAC TCTGCCCACT CCCAGGGAGA GGAACCTAGC ATCCCCATCA 
CCTTCCACTC TGGTTGAGGC AAGAGAGGTG GGGGAGGCAA CTGGTGGTCC TGAGCTATCT 
GGGGTCCCTC GAGGAGAGAG CGAGGAGACA GGAAGCTCCG AGGGTGCCCC TTCCCTGCTT 
CCAGCCACAC GGGCCCCTGA GGGTACCAGG GAGCTGGAGG CCCCCTCTGA AGATAATTCT 
GGAAGAACTG CCCCAGCAGG GACCTCAGTG CAGGCCCAGC CAGTGCTGCC CACTGACAGC 
GCCAGCCGAG GTGGAGTGGC CGTGGTCCCC GCATCAGGTG ACTGTGTCCC CAGCCCCTGC 
CACAATGGTG GGACATGCTT GGAGGAGGAG GAAGGGGTCC GCTGCCTATG TCTGCCTGGC 
TATGGGGGGG ACCTGTGCGA TGTTGGCCTC CGCTTCTGCA ACCCCGGCTG GGACGCCTTC 
CAGGGCGCCT GCTACAAGCA CTTTTCCACA CGAAGGAGCT GGGAGGAGGC AGAGACCCAG 
TGCCGGATGT ACGGCGCGCA TCTGGCCAGC ATCAGCACAC CCGAGGAACA GGACTTCATC 
AACAACCGGT ACCGGGAGTA CCAGTGGATC GGACTCAACG ACAGGACCAT CGAAGGCGAC 
TTCTTGTGGT CGGATGGCGT CCCCCTGCTC TATGAGAACT GGAACCCTGG GCAGCCTGAC 
AGCTACTTCC TGTCTGGAGA GAACTGCGTG GTCATGGTGT GGCATGATCA GGGACAATGG 
AGTGACGTGC CCTGCAACTA CCACCTGTCC TACACCTGCA AGATGGGGCT GGTGTCCTGT 
GGGCCGCCAC CGGAGCTGCC CCTGGCTCAA GTGTTCGGCC GCCCACGGCT GCGCTATGAG 
GTGGACACTG TGCTTCGCTA CCGGTGCCGG GAAGGACTGG CCCAGCGCAA TCTGCCGCTG 
ATCCGATGCC AAGAGAACGG TCGTTGGGAG GCCCCCCAGA TCTCCTGTGT GCCCAGAAGA 
CCTGCCCGAG CTCTGCACCC AGAGGAGGAC CCAGAAGGAC GTCAGGGGAG GCTACTGGGA 
CGCTGGAAGG CGCTGTTGAT CCCCCCTTCC AGCCCCATGC CAGGTCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857536.1
CDS	123..2993
Translation	MLGSKERSFLTNNGGEEVEERVGSGEAVLTCVRSRRPSWKRPACSMAQLFLPLLAALVLAQAPAALADVLEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPAYPASLTDVSLALSELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVYCFRDSAQPSAIPEASNPASNPASDGLEAIVTVTETLEELQLPQEATESESRGAIYSIPIMEDGGGGSSTPEDPAEAPRTLLEFETQSMVPPTGFSEEEGKALEEEEKYEDEEEKEEEEEEEEVEDEALWAWPSELSSPGPEASLPTEPAAQEESLSQAPARAVLQPGASPLPDGESEASRPPRVHGPPTETLPTPRERNLASPSPSTLVEAREVGEATGGPELSGVPRGESEETGSSEGAPSLLPATRAPEGTRELEAPSEDNSGRTAPAGTSVQAQPVLPTDSASRGGVAVVPASGDCVPSPCHNGGTCLEEEEGVRCLCLPGYGGDLCDVGLRFCNPGWDAFQGACYKHFSTRRSWEEAETQCRMYGAHLASISTPEEQDFINNRYREYQWIGLNDRTIEGDFLWSDGVPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQWSDVPCNYHLSYTCKMGLVSCGPPPELPLAQVFGRPRLRYEVDTVLRYRCREGLAQRNLPLIRCQENGRWEAPQISCVPRRPARALHPEEDPEGRQGRLLGRWKALLIPPSSPMPGP

Target ORF information:

RefSeq Version	XM_017002047.1
Organism	Homo sapiens(human)
Definition	Homo sapiens brevican (BCAN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002047.1

ORF Insert Sequence:

ATGTTGGGGA GTAAAGAGAG GAGTTTCCTT ACAAATAATG GTGGGGAGGA AGTAGAGGAG 
AGGGTGGGCT CAGGAGAAGC TGTGCTTACT TGCGTTCGGT CTAGGAGGCC CTCCTGGAAA 
AGGCCAGCCT GCAGCATGGC CCAGCTGTTC CTGCCCCTGC TGGCAGCCCT GGTCCTGGCC 
CAGGCTCCTG CAGCTTTAGC AGATGTTCTG GAAGGAGACA GCTCAGAGGA CCGCGCTTTT 
CGCGTGCGCA TCGCGGGCGA CGCGCCACTG CAGGGCGTGC TCGGCGGCGC CCTCACCATC 
CCTTGCCACG TCCACTACCT GCGGCCACCG CCGAGCCGCC GGGCTGTGCT GGGCTCTCCG 
CGGGTCAAGT GGACTTTCCT GTCCCGGGGC CGGGAGGCAG AGGTGCTGGT GGCGCGGGGA 
GTGCGCGTCA AGGTGAACGA GGCCTACCGG TTCCGCGTGG CACTGCCTGC GTACCCAGCG 
TCGCTCACCG ACGTCTCCCT GGCGCTGAGC GAGCTGCGCC CCAACGACTC AGGTATCTAT 
CGCTGTGAGG TCCAGCACGG CATCGATGAC AGCAGCGACG CTGTGGAGGT CAAGGTCAAA 
GGGGTCGTCT TTCTCTACCG AGAGGGCTCT GCCCGCTATG CTTTCTCCTT TTCTGGGGCC 
CAGGAGGCCT GTGCCCGCAT TGGAGCCCAC ATCGCCACCC CGGAGCAGCT CTATGCCGCC 
TACCTTGGGG GCTATGAGCA ATGTGATGCT GGCTGGCTGT CGGATCAGAC CGTGAGGTAT 
CCCATCCAGA CCCCACGAGA GGCCTGTTAC GGAGACATGG ATGGCTTCCC CGGGGTCCGG 
AACTATGGTG TGGTGGACCC GGATGACCTC TATGATGTGT ACTGTTATGC TGAAGACCTA 
AATGGAGAAC TGTTCCTGGG TGACCCTCCA GAGAAGCTGA CATTGGAGGA AGCACGGGCG 
TACTGCCAGG AGCGGGGTGC AGAGATTGCC ACCACGGGCC AACTGTATGC AGCCTGGGAT 
GGTGGCCTGG ACCACTGCAG CCCAGGGTGG CTAGCTGATG GCAGTGTGCG CTACCCCATC 
GTCACACCCA GCCAGCGCTG TGGTGGGGGC TTGCCTGGTG TCAAGACTCT CTTCCTCTTC 
CCCAACCAGA CTGGCTTCCC CAATAAGCAC AGCCGCTTCA ACGTCTACTG CTTCCGAGAC 
TCGGCCCAGC CTTCTGCCAT CCCTGAGGCC TCCAACCCAG CCTCCAACCC AGCCTCTGAT 
GGACTAGAGG CTATCGTCAC AGTGACAGAG ACCCTGGAGG AACTGCAGCT GCCTCAGGAA 
GCCACAGAGA GTGAATCCCG TGGGGCCATC TACTCCATCC CCATCATGGA GGACGGAGGA 
GGTGGAAGCT CCACTCCAGA AGACCCAGCA GAGGCCCCTA GGACGCTCCT AGAATTTGAA 
ACACAATCCA TGGTACCGCC CACGGGGTTC TCAGAAGAGG AAGGTAAGGC ATTGGAGGAA 
GAAGAGAAAT ATGAAGATGA AGAAGAGAAA GAGGAGGAAG AAGAAGAGGA GGAGGTGGAG 
GATGAGGCTC TGTGGGCATG GCCCAGCGAG CTCAGCAGCC CGGGCCCTGA GGCCTCTCTC 
CCCACTGAGC CAGCAGCCCA GGAGGAGTCA CTCTCCCAGG CGCCAGCAAG GGCAGTCCTG 
CAGCCTGGTG CATCACCACT TCCTGATGGA GAGTCAGAAG CTTCCAGGCC TCCAAGGGTC 
CATGGACCAC CTACTGAGAC TCTGCCCACT CCCAGGGAGA GGAACCTAGC ATCCCCATCA 
CCTTCCACTC TGGTTGAGGC AAGAGAGGTG GGGGAGGCAA CTGGTGGTCC TGAGCTATCT 
GGGGTCCCTC GAGGAGAGAG CGAGGAGACA GGAAGCTCCG AGGGTGCCCC TTCCCTGCTT 
CCAGCCACAC GGGCCCCTGA GGGTACCAGG GAGCTGGAGG CCCCCTCTGA AGATAATTCT 
GGAAGAACTG CCCCAGCAGG GACCTCAGTG CAGGCCCAGC CAGTGCTGCC CACTGACAGC 
GCCAGCCGAG GTGGAGTGGC CGTGGTCCCC GCATCAGGTG ACTGTGTCCC CAGCCCCTGC 
CACAATGGTG GGACATGCTT GGAGGAGGAG GAAGGGGTCC GCTGCCTATG TCTGCCTGGC 
TATGGGGGGG ACCTGTGCGA TGTTGGCCTC CGCTTCTGCA ACCCCGGCTG GGACGCCTTC 
CAGGGCGCCT GCTACAAGCA CTTTTCCACA CGAAGGAGCT GGGAGGAGGC AGAGACCCAG 
TGCCGGATGT ACGGCGCGCA TCTGGCCAGC ATCAGCACAC CCGAGGAACA GGACTTCATC 
AACAACCGGT ACCGGGAGTA CCAGTGGATC GGACTCAACG ACAGGACCAT CGAAGGCGAC 
TTCTTGTGGT CGGATGGCGT CCCCCTGCTC TATGAGAACT GGAACCCTGG GCAGCCTGAC 
AGCTACTTCC TGTCTGGAGA GAACTGCGTG GTCATGGTGT GGCATGATCA GGGACAATGG 
AGTGACGTGC CCTGCAACTA CCACCTGTCC TACACCTGCA AGATGGGGCT GGTGTCCTGT 
GGGCCGCCAC CGGAGCTGCC CCTGGCTCAA GTGTTCGGCC GCCCACGGCT GCGCTATGAG 
GTGGACACTG TGCTTCGCTA CCGGTGCCGG GAAGGACTGG CCCAGCGCAA TCTGCCGCTG 
ATCCGATGCC AAGAGAACGG TCGTTGGGAG GCCCCCCAGA TCTCCTGTGT GCCCAGAAGA 
CCTGCCCGAG CTCTGCACCC AGAGGAGGAC CCAGAAGGAC GTCAGGGGAG GCTACTGGGA 
CGCTGGAAGG CGCTGTTGAT CCCCCCTTCC AGCCCCATGC CAGGTCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide methylation screen in low-grade breast cancer identifies novel epigenetically altered genes as potential biomarkers for tumor diagnosis.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(12)4937-50(2012 Dec)
Faryna M,Konermann C,Aulmann S,Bermejo JL,Brugger M,Diederichs S,Rom J,Weichenhan D,Claus R,Rehli M,Schirmacher P,Sinn HP,Plass C,Gerhauser C

Brevican, neurocan, tenascin-C and versican are mainly responsible for the invasiveness of low-grade astrocytoma.
Pathology oncology research : POR18(2)413-20(2012 Apr)
Varga I,Hut?czki G,Szemcs?k CD,Zahuczky G,T?th J,Adamecz Z,Kenyeres A,Bogn?r L,Hanz?ly Z,Klekner A

Candidate gene analysis of the human natural killer-1 carbohydrate pathway and perineuronal nets in schizophrenia: B3GAT2 is associated with disease risk and cortical surface area.
Biological psychiatry69(1)90-6(2011 Jan)
K?hler AK,Djurovic S,Rimol LM,Brown AA,Athanasiu L,J?nsson EG,Hansen T,G?stafsson O,Hall H,Giegling I,Muglia P,Cichon S,Rietschel M,Pietil?inen OP,Peltonen L,Bramon E,Collier D,St Clair D,Sigurdsson E,Petursson H,Rujescu D,Melle I,Werge T,Steen VM,Dale AM,Matthews RT,Agartz I,Andreassen OA

The proteoglycan brevican binds to fibronectin after proteolytic cleavage and promotes glioma cell motility.
The Journal of biological chemistry283(36)24848-59(2008 Sep)
Hu B,Kong LL,Matthews RT,Viapiano MS

Human glioblastomas overexpress ADAMTS-5 that degrades brevican.
Acta neuropathologica110(3)239-46(2005 Sep)
Nakada M,Miyamori H,Kita D,Takahashi T,Yamashita J,Sato H,Miura R,Yamaguchi Y,Okada Y

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BCAN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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