CCL8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCL8
Entrez Gene ID	6355
Full Name	C-C motif chemokine ligand 8
Synonyms	HC14,MCP-2,MCP2,SCYA10,SCYA8
General protein information	Preferred Names C-C motif chemokine ligand 8 Names C-C motif chemokine 8 chemokine (C-C motif) ligand 8 monocyte chemoattractant protein 2 monocyte chemotactic protein 2 small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2) small-inducible cytokine A8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q12
Summary	This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014].
Disorder MIM:	602283

mRNA and Protein(s)

mRNA	Protein	Name
NM_005623.3	NP_005614.2	C-C motif chemokine 8 precursor
NM_005623.2	NP_005614.2	C-C motif chemokine 8 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CCL8	NP_005614.2
Bos taurus (cattle)	CCL8	NP_776432.1
Pan troglodytes (chimpanzee)	CCL8	XP_523600.2
	CCL8	NP_001028023.1
Canis lupus familiaris (dog)	CCL8	NP_001005255.1

Gene Ontology
Bibliography

Related articles in PubMed

MCP2 activates NF-?B signaling pathway promoting the migration and invasion of ESCC cells.
Zhou J, Zheng S, Liu T, Liu Q, Chen Y, Tan D, Ma R, Lu X
Cell biology international(2017 Nov)

Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation.
von Hundelshausen P, Agten SM, Eckardt V, Blanchet X, Schmitt MM, Ippel H, Neideck C, Bidzhekov K, Leberzammer J, Wichapong K, Faussner A, Drechsler M, Grommes J, van Geffen JP, Li H, Ortega-Gomez A, Megens RT, Naumann R, Dijkgraaf I, Nicolaes GA, D?ring Y, Soehnlein O, Lutgens E, Heemskerk JW, Koenen RR, Mayo KH, Hackeng TM, Weber C
Science translational medicine9(384)(2017 Apr)

Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis.
Lee JU, Cheong HS, Shim EY, Bae DJ, Chang HS, Uh ST, Kim YH, Park JS, Lee B, Shin HD, Park CS
Respiratory research18(1)3(2017 Jan)

A CCL8 gradient drives breast cancer cell dissemination.
Farmaki E, Chatzistamou I, Kaza V, Kiaris H
Oncogene35(49)6309-6318(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings exemplify how gradients of chemoattractive factors such as CCL8, drive metastasis and suggest that interference with their operation may provide means for breast cancer management.
Title: A CCL8 gradient drives breast cancer cell dissemination.

Detecting expression changes in TLR4 and MCP2 in the peripheral blood is a feasible method for predicting the occurrence of abortion in women of child-bearing age
Title: Relationship between TLR4 and MCP2 expression levels and habitual abortion.

Dermal fibroblast CCL8 promotes melanoma metastasis.
Title: The importance of microenvironment: the role of CCL8 in metastasis formation of melanoma.

Authors show that the previously observed downregulation of hsa-miR-92a and upregulation of CCL8 during human cytomegalovirus latent infection of myeloid cells are intimately linked via the latency-associated expression of cytomegalovirus UL111A.
Title: Latency-associated viral interleukin-10 (IL-10) encoded by human cytomegalovirus modulates cellular IL-10 and CCL8 Secretion during latent infection through changes in the cellular microRNA hsa-miR-92a.

Results indicate that the induction of MCP-2/CCL8 by mycobacteria is dependent on the activation of TLR2/PI3K/Akt signaling pathway.
Title: Induction of CCL8/MCP-2 by mycobacteria through the activation of TLR2/PI3K/Akt signaling pathway.

The following CCL8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCL8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13214	NM_005623.3 Latest version!	Homo sapiens C-C motif chemokine ligand 8 (CCL8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13214	NM_005623.2	Homo sapiens C-C motif chemokine ligand 8 (CCL8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCL8 ( NM_005623.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005614.2 Homo sapiens C-C motif chemokine ligand 8 (CCL8), mRNA.

Price & Availability↑

CloneID	OHu13214
Clone ID Related Accession (Same CDS sequence)	NM_005623.3 , NM_005623.2
Accession Version	NM_005623.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-25
Organism	Homo sapiens(human)
Product	C-C motif chemokine 8 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011193.3. On Nov 26, 2018 this sequence version replaced NM_005623.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y16645.1, AV733664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved MANE Ensembl match :: ENST00000394620.2/ ENSP00000378118.1 Protein has antimicrobial activity :: PMID: 12949249 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGGTTT CTGCAGCGCT TCTGTGCCTG CTGCTCATGG CAGCCACTTT CAGCCCTCAG 
GGACTTGCTC AGCCAGATTC AGTTTCCATT CCAATCACCT GCTGCTTTAA CGTGATCAAT 
AGGAAAATTC CTATCCAGAG GCTGGAGAGC TACACAAGAA TCACCAACAT CCAATGTCCC 
AAGGAAGCTG TGATCTTCAA GACCAAACGG GGCAAGGAGG TCTGTGCTGA CCCCAAGGAG 
AGATGGGTCA GGGATTCCAT GAAGCATCTG GACCAAATAT TTCAAAATCT GAAGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005614.2
CDS	68..367
Translation	MKVSAALLCLLLMAATFSPQGLAQPDSVSIPITCCFNVINRKIPIQRLESYTRITNIQCPKEAVIFKTKRGKEVCADPKERWVRDSMKHLDQIFQNLKP

Target ORF information:

RefSeq Version	NM_005623.3
Organism	Homo sapiens(human)
Definition	Homo sapiens C-C motif chemokine ligand 8 (CCL8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005623.3

ORF Insert Sequence:

ATGAAGGTTT CTGCAGCGCT TCTGTGCCTG CTGCTCATGG CAGCCACTTT CAGCCCTCAG 
GGACTTGCTC AGCCAGATTC AGTTTCCATT CCAATCACCT GCTGCTTTAA CGTGATCAAT 
AGGAAAATTC CTATCCAGAG GCTGGAGAGC TACACAAGAA TCACCAACAT CCAATGTCCC 
AAGGAAGCTG TGATCTTCAA GACCAAACGG GGCAAGGAGG TCTGTGCTGA CCCCAAGGAG 
AGATGGGTCA GGGATTCCAT GAAGCATCTG GACCAAATAT TTCAAAATCT GAAGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCL8 ( NM_005623.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005614.2 Homo sapiens C-C motif chemokine ligand 8 (CCL8), mRNA.

Price & Availability↑

CloneID	OHu13214
Clone ID Related Accession (Same CDS sequence)	NM_005623.3 , NM_005623.2
Accession Version	NM_005623.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	C-C motif chemokine 8 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Y16645.1. On Aug 29, 2002 this sequence version replaced NM_005623.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y16645.1, AV733664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved Protein has antimicrobial activity :: PMID: 12949249 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTTT CTGCAGCGCT TCTGTGCCTG CTGCTCATGG CAGCCACTTT CAGCCCTCAG 
GGACTTGCTC AGCCAGATTC AGTTTCCATT CCAATCACCT GCTGCTTTAA CGTGATCAAT 
AGGAAAATTC CTATCCAGAG GCTGGAGAGC TACACAAGAA TCACCAACAT CCAATGTCCC 
AAGGAAGCTG TGATCTTCAA GACCAAACGG GGCAAGGAGG TCTGTGCTGA CCCCAAGGAG 
AGATGGGTCA GGGATTCCAT GAAGCATCTG GACCAAATAT TTCAAAATCT GAAGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005614.2
CDS	456..755
Translation	MKVSAALLCLLLMAATFSPQGLAQPDSVSIPITCCFNVINRKIPIQRLESYTRITNIQCPKEAVIFKTKRGKEVCADPKERWVRDSMKHLDQIFQNLKP

Target ORF information:

RefSeq Version	NM_005623.2
Organism	Homo sapiens(human)
Definition	Homo sapiens C-C motif chemokine ligand 8 (CCL8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005623.2

ORF Insert Sequence:

ATGAAGGTTT CTGCAGCGCT TCTGTGCCTG CTGCTCATGG CAGCCACTTT CAGCCCTCAG 
GGACTTGCTC AGCCAGATTC AGTTTCCATT CCAATCACCT GCTGCTTTAA CGTGATCAAT 
AGGAAAATTC CTATCCAGAG GCTGGAGAGC TACACAAGAA TCACCAACAT CCAATGTCCC 
AAGGAAGCTG TGATCTTCAA GACCAAACGG GGCAAGGAGG TCTGTGCTGA CCCCAAGGAG 
AGATGGGTCA GGGATTCCAT GAAGCATCTG GACCAAATAT TTCAAAATCT GAAGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MCP2 activates NF-?B signaling pathway promoting the migration and invasion of ESCC cells.
Cell biology international(2017 Nov)
Zhou J,Zheng S,Liu T,Liu Q,Chen Y,Tan D,Ma R,Lu X

Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation.
Science translational medicine9(384)(2017 Apr)
von Hundelshausen P,Agten SM,Eckardt V,Blanchet X,Schmitt MM,Ippel H,Neideck C,Bidzhekov K,Leberzammer J,Wichapong K,Faussner A,Drechsler M,Grommes J,van Geffen JP,Li H,Ortega-Gomez A,Megens RT,Naumann R,Dijkgraaf I,Nicolaes GA,D?ring Y,Soehnlein O,Lutgens E,Heemskerk JW,Koenen RR,Mayo KH,Hackeng TM,Weber C

Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis.
Respiratory research18(1)3(2017 Jan)
Lee JU,Cheong HS,Shim EY,Bae DJ,Chang HS,Uh ST,Kim YH,Park JS,Lee B,Shin HD,Park CS

A CCL8 gradient drives breast cancer cell dissemination.
Oncogene35(49)6309-6318(2016 Dec)
Farmaki E,Chatzistamou I,Kaza V,Kiaris H

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CCL8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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