SCNN1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCNN1A
Entrez Gene ID	6337
Full Name	sodium channel epithelial 1 alpha subunit
Synonyms	BESC2,ENaCa,ENaCalpha,SCNEA,SCNN1
General protein information	Preferred Names sodium channel epithelial 1 alpha subunit Names amiloride-sensitive sodium channel subunit alpha alpha ENaC-2 alpha-ENaC alpha-NaCH amiloride-sensitive epithelial sodium channel alpha subunit amiloride-sensitive sodium channel subunit alpha 2 epithelial Na(+) channel subunit alpha nasal epithelial sodium channel alpha subunit nonvoltage-gated sodium channel 1 subunit alpha sodium channel, non voltage gated 1 alpha subunit sodium channel, non-voltage-gated 1 alpha sodium channel, nonvoltage-gated 1 alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.31
Summary	Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009].
Disorder MIM:	600228
Disorder Html:	Pseudohypoaldosteronism, type I, 264350 (3); Bronchiectasis with or without elevated sweat chloride 2, 613021 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001159576.2	NP_001153048.1	amiloride-sensitive sodium channel subunit alpha isoform 2
NM_001038.6	NP_001029.1	amiloride-sensitive sodium channel subunit alpha isoform 1
NM_001159575.1	NP_001153047.1	amiloride-sensitive sodium channel subunit alpha isoform 3
NM_001159576.1	NP_001153048.1	amiloride-sensitive sodium channel subunit alpha isoform 2
NM_001038.5	NP_001029.1	amiloride-sensitive sodium channel subunit alpha isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SCNN1A	XP_005637310.1
Rattus norvegicus (Norway rat)	Scnn1a	NP_113736.1
	SCNN1A	XP_001103017.2
Xenopus tropicalis (tropical clawed frog)	scnn1a	NP_001184124.1
Bos taurus (cattle)	SCNN1A	NP_777023.1
Mus musculus (house mouse)	Scnn1a	NP_035454.2
Homo sapiens (human)	SCNN1A	NP_001153047.1
Pan troglodytes (chimpanzee)	SCNN1A	XP_508948.4
Gallus gallus (chicken)	SCNN1A	NP_990476.2

Gene Ontology
Bibliography

Related articles in PubMed

Mapping allosteric linkage to channel gating of extracellular domains in the human epithelial sodium channel.
Shobair M, Popov KI, Dang YL, He H, Stutts MJ, Dokholyan NV
The Journal of biological chemistry(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutation in the extracellular domain of alphaENaC causes Liddle syndrome by increasing intrinsic channel activity.
Title: A Missense Mutation in the Extracellular Domain of

The derlin-1 pathway therefore may represent a significant early checkpoint in the recognition and degradation of ENaC in mammalian cells.
Title: Derlin-1 promotes ubiquitylation and degradation of the epithelial Na

The data indicate that the Lhs1 chaperone selectively recognizes an immature form of alphaENaC, one which has failed to correctly assemble with the other channel subunits via its transmembrane domain.
Title: Interactions between intersubunit transmembrane domains regulate the chaperone-dependent degradation of an oligomeric membrane protein.

Data show that the expression of aquaporin (AQP) 1, AQP3, AQP5, epithelial Na+ channel (ENaC) and sodium potassium ATPase (Na-K-ATPase) are altered in patients with acute respiratory failure (ARF) due to diffuse alveolar damage (DAD), and the cause of DAD does not seem to influence the level of impairment of these channels.
Title: The Expression of Water and Ion Channels in Diffuse Alveolar Damage Is Not Dependent on DAD Etiology.

LEFTY2 regulates the expression and activity of ENaC in endometrial epithelial cells via SGK1.
Title: LEFTYA Activates the Epithelial Na+ Channel (ENaC) in Endometrial Cells via Serum and Glucocorticoid Inducible Kinase SGK1.

The following SCNN1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCNN1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14548	NM_001159576.2 Latest version!	Homo sapiens sodium channel epithelial 1 subunit alpha (SCNN1A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$369.50-$517.30 $739.00
OHu19374	NM_001038.6 Latest version!	Homo sapiens sodium channel epithelial 1 subunit alpha (SCNN1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu14485	NM_001159575.1 Latest version!	Homo sapiens sodium channel epithelial 1 subunit alpha (SCNN1A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$489.30 $699.00
View Old Accession Versions >>
OHu14548	NM_001159576.1	Homo sapiens sodium channel epithelial 1 alpha subunit (SCNN1A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$369.50-$517.30 $739.00
OHu19374	NM_001038.5	Homo sapiens sodium channel epithelial 1 alpha subunit (SCNN1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCNN1A ( NM_001159576.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153048.1 Homo sapiens sodium channel epithelial 1 subunit alpha (SCNN1A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14548
Clone ID Related Accession (Same CDS sequence)	NM_001159576.2 , NM_001159576.1
Accession Version	NM_001159576.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	amiloride-sensitive sodium channel subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005840.2, AK172792.1 and BC006526.2. On May 31, 2019 this sequence version replaced NM_001159576.1. Transcript Variant: This variant (2, also known as alpha-ENaC2) contains an alternate segment in the 5' coding region, and uses an in-frame upstream start codon compared to variant 1. This results in an isoform (2) with a longer N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1629623.1, AK172792.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCATGG CCAGGGGCAG CCTCACTCGG GTTCCAGGGG TGATGGGAGA GGGCACTCAG 
GGCCCAGAGC TCAGCCTTGA CCCTGACCCT TGCTCTCCCC AATCCACTCC GGGGCTCATG 
AAGGGGAACA AGCTGGAGGA GCAGGACCCT AGACCTCTGC AGCCCATACC AGGTCTCATG 
GAGGGGAACA AGCTGGAGGA GCAGGACTCT AGCCCTCCAC AGTCCACTCC AGGGCTCATG 
AAGGGGAACA AGCGTGAGGA GCAGGGGCTG GGCCCCGAAC CTGCGGCGCC CCAGCAGCCC 
ACGGCGGAGG AGGAGGCCCT GATCGAGTTC CACCGCTCCT ACCGAGAGCT CTTCGAGTTC 
TTCTGCAACA ACACCACCAT CCACGGCGCC ATCCGCCTGG TGTGCTCCCA GCACAACCGC 
ATGAAGACGG CCTTCTGGGC AGTGCTGTGG CTCTGCACCT TTGGCATGAT GTACTGGCAA 
TTCGGCCTGC TTTTCGGAGA GTACTTCAGC TACCCCGTCA GCCTCAACAT CAACCTCAAC 
TCGGACAAGC TCGTCTTCCC CGCAGTGACC ATCTGCACCC TCAATCCCTA CAGGTACCCG 
GAAATTAAAG AGGAGCTGGA GGAGCTGGAC CGCATCACAG AGCAGACGCT CTTTGACCTG 
TACAAATACA GCTCCTTCAC CACTCTCGTG GCCGGCTCCC GCAGCCGTCG CGACCTGCGG 
GGGACTCTGC CGCACCCCTT GCAGCGCCTG AGGGTCCCGC CCCCGCCTCA CGGGGCCCGT 
CGAGCCCGTA GCGTGGCCTC CAGCTTGCGG GACAACAACC CCCAGGTGGA CTGGAAGGAC 
TGGAAGATCG GCTTCCAGCT GTGCAACCAG AACAAATCGG ACTGCTTCTA CCAGACATAC 
TCATCAGGGG TGGATGCGGT GAGGGAGTGG TACCGCTTCC ACTACATCAA CATCCTGTCG 
AGGCTGCCAG AGACTCTGCC ATCCCTGGAG GAGGACACGC TGGGCAACTT CATCTTCGCC 
TGCCGCTTCA ACCAGGTCTC CTGCAACCAG GCGAATTACT CTCACTTCCA CCACCCGATG 
TATGGAAACT GCTATACTTT CAATGACAAG AACAACTCCA ACCTCTGGAT GTCTTCCATG 
CCTGGAATCA ACAACGGTCT GTCCCTGATG CTGCGCGCAG AGCAGAATGA CTTCATTCCC 
CTGCTGTCCA CAGTGACTGG GGCCCGGGTA ATGGTGCACG GGCAGGATGA ACCTGCCTTT 
ATGGATGATG GTGGCTTTAA CTTGCGGCCT GGCGTGGAGA CCTCCATCAG CATGAGGAAG 
GAAACCCTGG ACAGACTTGG GGGCGATTAT GGCGACTGCA CCAAGAATGG CAGTGATGTT 
CCTGTTGAGA ACCTTTACCC TTCAAAGTAC ACACAGCAGG TGTGTATTCA CTCCTGCTTC 
CAGGAGAGCA TGATCAAGGA GTGTGGCTGT GCCTACATCT TCTATCCGCG GCCCCAGAAC 
GTGGAGTACT GTGACTACAG AAAGCACAGT TCCTGGGGGT ACTGCTACTA TAAGCTCCAG 
GTTGACTTCT CCTCAGACCA CCTGGGCTGT TTCACCAAGT GCCGGAAGCC ATGCAGCGTG 
ACCAGCTACC AGCTCTCTGC TGGTTACTCA CGATGGCCCT CGGTGACATC CCAGGAATGG 
GTCTTCCAGA TGCTATCGCG ACAGAACAAT TACACCGTCA ACAACAAGAG AAATGGAGTG 
GCCAAAGTCA ACATCTTCTT CAAGGAGCTG AACTACAAAA CCAATTCTGA GTCTCCCTCT 
GTCACGATGG TCACCCTCCT GTCCAACCTG GGCAGCCAGT GGAGCCTGTG GTTCGGCTCC 
TCGGTGTTGT CTGTGGTGGA GATGGCTGAG CTCGTCTTTG ACCTGCTGGT CATCATGTTC 
CTCATGCTGC TCCGAAGGTT CCGAAGCCGA TACTGGTCTC CAGGCCGAGG GGGCAGGGGT 
GCTCAGGAGG TAGCCTCCAC CCTGGCATCC TCCCCTCCTT CCCACTTCTG CCCCCACCCC 
ATGTCTCTGT CCTTGTCCCA GCCAGGCCCT GCTCCCTCTC CAGCCTTGAC AGCCCCTCCC 
CCTGCCTATG CCACCCTGGG CCCCCGCCCA TCTCCAGGGG GCTCTGCAGG GGCCAGTTCC 
TCCACCTGTC CTCTGGGGGG GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153048.1
CDS	491..2677
Translation	MGMARGSLTRVPGVMGEGTQGPELSLDPDPCSPQSTPGLMKGNKLEEQDPRPLQPIPGLMEGNKLEEQDSSPPQSTPGLMKGNKREEQGLGPEPAAPQQPTAEEEALIEFHRSYRELFEFFCNNTTIHGAIRLVCSQHNRMKTAFWAVLWLCTFGMMYWQFGLLFGEYFSYPVSLNINLNSDKLVFPAVTICTLNPYRYPEIKEELEELDRITEQTLFDLYKYSSFTTLVAGSRSRRDLRGTLPHPLQRLRVPPPPHGARRARSVASSLRDNNPQVDWKDWKIGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYINILSRLPETLPSLEEDTLGNFIFACRFNQVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAEQNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLYPSKYTQQVCIHSCFQESMIKECGCAYIFYPRPQNVEYCDYRKHSSWGYCYYKLQVDFSSDHLGCFTKCRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQNNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVFDLLVIMFLMLLRRFRSRYWSPGRGGRGAQEVASTLASSPPSHFCPHPMSLSLSQPGPAPSPALTAPPPAYATLGPRPSPGGSAGASSSTCPLGGP

Target ORF information:

RefSeq Version	NM_001159576.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium channel epithelial 1 subunit alpha (SCNN1A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159576.2

ORF Insert Sequence:

ATGGGCATGG CCAGGGGCAG CCTCACTCGG GTTCCAGGGG TGATGGGAGA GGGCACTCAG 
GGCCCAGAGC TCAGCCTTGA CCCTGACCCT TGCTCTCCCC AATCCACTCC GGGGCTCATG 
AAGGGGAACA AGCTGGAGGA GCAGGACCCT AGACCTCTGC AGCCCATACC AGGTCTCATG 
GAGGGGAACA AGCTGGAGGA GCAGGACTCT AGCCCTCCAC AGTCCACTCC AGGGCTCATG 
AAGGGGAACA AGCGTGAGGA GCAGGGGCTG GGCCCCGAAC CTGCGGCGCC CCAGCAGCCC 
ACGGCGGAGG AGGAGGCCCT GATCGAGTTC CACCGCTCCT ACCGAGAGCT CTTCGAGTTC 
TTCTGCAACA ACACCACCAT CCACGGCGCC ATCCGCCTGG TGTGCTCCCA GCACAACCGC 
ATGAAGACGG CCTTCTGGGC AGTGCTGTGG CTCTGCACCT TTGGCATGAT GTACTGGCAA 
TTCGGCCTGC TTTTCGGAGA GTACTTCAGC TACCCCGTCA GCCTCAACAT CAACCTCAAC 
TCGGACAAGC TCGTCTTCCC CGCAGTGACC ATCTGCACCC TCAATCCCTA CAGGTACCCG 
GAAATTAAAG AGGAGCTGGA GGAGCTGGAC CGCATCACAG AGCAGACGCT CTTTGACCTG 
TACAAATACA GCTCCTTCAC CACTCTCGTG GCCGGCTCCC GCAGCCGTCG CGACCTGCGG 
GGGACTCTGC CGCACCCCTT GCAGCGCCTG AGGGTCCCGC CCCCGCCTCA CGGGGCCCGT 
CGAGCCCGTA GCGTGGCCTC CAGCTTGCGG GACAACAACC CCCAGGTGGA CTGGAAGGAC 
TGGAAGATCG GCTTCCAGCT GTGCAACCAG AACAAATCGG ACTGCTTCTA CCAGACATAC 
TCATCAGGGG TGGATGCGGT GAGGGAGTGG TACCGCTTCC ACTACATCAA CATCCTGTCG 
AGGCTGCCAG AGACTCTGCC ATCCCTGGAG GAGGACACGC TGGGCAACTT CATCTTCGCC 
TGCCGCTTCA ACCAGGTCTC CTGCAACCAG GCGAATTACT CTCACTTCCA CCACCCGATG 
TATGGAAACT GCTATACTTT CAATGACAAG AACAACTCCA ACCTCTGGAT GTCTTCCATG 
CCTGGAATCA ACAACGGTCT GTCCCTGATG CTGCGCGCAG AGCAGAATGA CTTCATTCCC 
CTGCTGTCCA CAGTGACTGG GGCCCGGGTA ATGGTGCACG GGCAGGATGA ACCTGCCTTT 
ATGGATGATG GTGGCTTTAA CTTGCGGCCT GGCGTGGAGA CCTCCATCAG CATGAGGAAG 
GAAACCCTGG ACAGACTTGG GGGCGATTAT GGCGACTGCA CCAAGAATGG CAGTGATGTT 
CCTGTTGAGA ACCTTTACCC TTCAAAGTAC ACACAGCAGG TGTGTATTCA CTCCTGCTTC 
CAGGAGAGCA TGATCAAGGA GTGTGGCTGT GCCTACATCT TCTATCCGCG GCCCCAGAAC 
GTGGAGTACT GTGACTACAG AAAGCACAGT TCCTGGGGGT ACTGCTACTA TAAGCTCCAG 
GTTGACTTCT CCTCAGACCA CCTGGGCTGT TTCACCAAGT GCCGGAAGCC ATGCAGCGTG 
ACCAGCTACC AGCTCTCTGC TGGTTACTCA CGATGGCCCT CGGTGACATC CCAGGAATGG 
GTCTTCCAGA TGCTATCGCG ACAGAACAAT TACACCGTCA ACAACAAGAG AAATGGAGTG 
GCCAAAGTCA ACATCTTCTT CAAGGAGCTG AACTACAAAA CCAATTCTGA GTCTCCCTCT 
GTCACGATGG TCACCCTCCT GTCCAACCTG GGCAGCCAGT GGAGCCTGTG GTTCGGCTCC 
TCGGTGTTGT CTGTGGTGGA GATGGCTGAG CTCGTCTTTG ACCTGCTGGT CATCATGTTC 
CTCATGCTGC TCCGAAGGTT CCGAAGCCGA TACTGGTCTC CAGGCCGAGG GGGCAGGGGT 
GCTCAGGAGG TAGCCTCCAC CCTGGCATCC TCCCCTCCTT CCCACTTCTG CCCCCACCCC 
ATGTCTCTGT CCTTGTCCCA GCCAGGCCCT GCTCCCTCTC CAGCCTTGAC AGCCCCTCCC 
CCTGCCTATG CCACCCTGGG CCCCCGCCCA TCTCCAGGGG GCTCTGCAGG GGCCAGTTCC 
TCCACCTGTC CTCTGGGGGG GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCNN1A ( NM_001159576.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153048.1 Homo sapiens sodium channel epithelial 1 alpha subunit (SCNN1A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14548
Clone ID Related Accession (Same CDS sequence)	NM_001159576.2 , NM_001159576.1
Accession Version	NM_001159576.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	amiloride-sensitive sodium channel subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK172792.1, BC006526.2 and BU680600.1. Transcript Variant: This variant (2, also known as alpha-ENaC2) contains an alternate segment in the 5' coding region, and uses an in-frame upstream start codon compared to variant 1. This results in an isoform (2) with a longer N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK172792.1, BC006526.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCATGG CCAGGGGCAG CCTCACTCGG GTTCCAGGGG TGATGGGAGA GGGCACTCAG 
GGCCCAGAGC TCAGCCTTGA CCCTGACCCT TGCTCTCCCC AATCCACTCC GGGGCTCATG 
AAGGGGAACA AGCTGGAGGA GCAGGACCCT AGACCTCTGC AGCCCATACC AGGTCTCATG 
GAGGGGAACA AGCTGGAGGA GCAGGACTCT AGCCCTCCAC AGTCCACTCC AGGGCTCATG 
AAGGGGAACA AGCGTGAGGA GCAGGGGCTG GGCCCCGAAC CTGCGGCGCC CCAGCAGCCC 
ACGGCGGAGG AGGAGGCCCT GATCGAGTTC CACCGCTCCT ACCGAGAGCT CTTCGAGTTC 
TTCTGCAACA ACACCACCAT CCACGGCGCC ATCCGCCTGG TGTGCTCCCA GCACAACCGC 
ATGAAGACGG CCTTCTGGGC AGTGCTGTGG CTCTGCACCT TTGGCATGAT GTACTGGCAA 
TTCGGCCTGC TTTTCGGAGA GTACTTCAGC TACCCCGTCA GCCTCAACAT CAACCTCAAC 
TCGGACAAGC TCGTCTTCCC CGCAGTGACC ATCTGCACCC TCAATCCCTA CAGGTACCCG 
GAAATTAAAG AGGAGCTGGA GGAGCTGGAC CGCATCACAG AGCAGACGCT CTTTGACCTG 
TACAAATACA GCTCCTTCAC CACTCTCGTG GCCGGCTCCC GCAGCCGTCG CGACCTGCGG 
GGGACTCTGC CGCACCCCTT GCAGCGCCTG AGGGTCCCGC CCCCGCCTCA CGGGGCCCGT 
CGAGCCCGTA GCGTGGCCTC CAGCTTGCGG GACAACAACC CCCAGGTGGA CTGGAAGGAC 
TGGAAGATCG GCTTCCAGCT GTGCAACCAG AACAAATCGG ACTGCTTCTA CCAGACATAC 
TCATCAGGGG TGGATGCGGT GAGGGAGTGG TACCGCTTCC ACTACATCAA CATCCTGTCG 
AGGCTGCCAG AGACTCTGCC ATCCCTGGAG GAGGACACGC TGGGCAACTT CATCTTCGCC 
TGCCGCTTCA ACCAGGTCTC CTGCAACCAG GCGAATTACT CTCACTTCCA CCACCCGATG 
TATGGAAACT GCTATACTTT CAATGACAAG AACAACTCCA ACCTCTGGAT GTCTTCCATG 
CCTGGAATCA ACAACGGTCT GTCCCTGATG CTGCGCGCAG AGCAGAATGA CTTCATTCCC 
CTGCTGTCCA CAGTGACTGG GGCCCGGGTA ATGGTGCACG GGCAGGATGA ACCTGCCTTT 
ATGGATGATG GTGGCTTTAA CTTGCGGCCT GGCGTGGAGA CCTCCATCAG CATGAGGAAG 
GAAACCCTGG ACAGACTTGG GGGCGATTAT GGCGACTGCA CCAAGAATGG CAGTGATGTT 
CCTGTTGAGA ACCTTTACCC TTCAAAGTAC ACACAGCAGG TGTGTATTCA CTCCTGCTTC 
CAGGAGAGCA TGATCAAGGA GTGTGGCTGT GCCTACATCT TCTATCCGCG GCCCCAGAAC 
GTGGAGTACT GTGACTACAG AAAGCACAGT TCCTGGGGGT ACTGCTACTA TAAGCTCCAG 
GTTGACTTCT CCTCAGACCA CCTGGGCTGT TTCACCAAGT GCCGGAAGCC ATGCAGCGTG 
ACCAGCTACC AGCTCTCTGC TGGTTACTCA CGATGGCCCT CGGTGACATC CCAGGAATGG 
GTCTTCCAGA TGCTATCGCG ACAGAACAAT TACACCGTCA ACAACAAGAG AAATGGAGTG 
GCCAAAGTCA ACATCTTCTT CAAGGAGCTG AACTACAAAA CCAATTCTGA GTCTCCCTCT 
GTCACGATGG TCACCCTCCT GTCCAACCTG GGCAGCCAGT GGAGCCTGTG GTTCGGCTCC 
TCGGTGTTGT CTGTGGTGGA GATGGCTGAG CTCGTCTTTG ACCTGCTGGT CATCATGTTC 
CTCATGCTGC TCCGAAGGTT CCGAAGCCGA TACTGGTCTC CAGGCCGAGG GGGCAGGGGT 
GCTCAGGAGG TAGCCTCCAC CCTGGCATCC TCCCCTCCTT CCCACTTCTG CCCCCACCCC 
ATGTCTCTGT CCTTGTCCCA GCCAGGCCCT GCTCCCTCTC CAGCCTTGAC AGCCCCTCCC 
CCTGCCTATG CCACCCTGGG CCCCCGCCCA TCTCCAGGGG GCTCTGCAGG GGCCAGTTCC 
TCCACCTGTC CTCTGGGGGG GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153048.1
CDS	265..2451
Translation	MGMARGSLTRVPGVMGEGTQGPELSLDPDPCSPQSTPGLMKGNKLEEQDPRPLQPIPGLMEGNKLEEQDSSPPQSTPGLMKGNKREEQGLGPEPAAPQQPTAEEEALIEFHRSYRELFEFFCNNTTIHGAIRLVCSQHNRMKTAFWAVLWLCTFGMMYWQFGLLFGEYFSYPVSLNINLNSDKLVFPAVTICTLNPYRYPEIKEELEELDRITEQTLFDLYKYSSFTTLVAGSRSRRDLRGTLPHPLQRLRVPPPPHGARRARSVASSLRDNNPQVDWKDWKIGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYINILSRLPETLPSLEEDTLGNFIFACRFNQVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAEQNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLYPSKYTQQVCIHSCFQESMIKECGCAYIFYPRPQNVEYCDYRKHSSWGYCYYKLQVDFSSDHLGCFTKCRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQNNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVFDLLVIMFLMLLRRFRSRYWSPGRGGRGAQEVASTLASSPPSHFCPHPMSLSLSQPGPAPSPALTAPPPAYATLGPRPSPGGSAGASSSTCPLGGP

Target ORF information:

RefSeq Version	NM_001159576.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium channel epithelial 1 alpha subunit (SCNN1A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159576.1

ORF Insert Sequence:

ATGGGCATGG CCAGGGGCAG CCTCACTCGG GTTCCAGGGG TGATGGGAGA GGGCACTCAG 
GGCCCAGAGC TCAGCCTTGA CCCTGACCCT TGCTCTCCCC AATCCACTCC GGGGCTCATG 
AAGGGGAACA AGCTGGAGGA GCAGGACCCT AGACCTCTGC AGCCCATACC AGGTCTCATG 
GAGGGGAACA AGCTGGAGGA GCAGGACTCT AGCCCTCCAC AGTCCACTCC AGGGCTCATG 
AAGGGGAACA AGCGTGAGGA GCAGGGGCTG GGCCCCGAAC CTGCGGCGCC CCAGCAGCCC 
ACGGCGGAGG AGGAGGCCCT GATCGAGTTC CACCGCTCCT ACCGAGAGCT CTTCGAGTTC 
TTCTGCAACA ACACCACCAT CCACGGCGCC ATCCGCCTGG TGTGCTCCCA GCACAACCGC 
ATGAAGACGG CCTTCTGGGC AGTGCTGTGG CTCTGCACCT TTGGCATGAT GTACTGGCAA 
TTCGGCCTGC TTTTCGGAGA GTACTTCAGC TACCCCGTCA GCCTCAACAT CAACCTCAAC 
TCGGACAAGC TCGTCTTCCC CGCAGTGACC ATCTGCACCC TCAATCCCTA CAGGTACCCG 
GAAATTAAAG AGGAGCTGGA GGAGCTGGAC CGCATCACAG AGCAGACGCT CTTTGACCTG 
TACAAATACA GCTCCTTCAC CACTCTCGTG GCCGGCTCCC GCAGCCGTCG CGACCTGCGG 
GGGACTCTGC CGCACCCCTT GCAGCGCCTG AGGGTCCCGC CCCCGCCTCA CGGGGCCCGT 
CGAGCCCGTA GCGTGGCCTC CAGCTTGCGG GACAACAACC CCCAGGTGGA CTGGAAGGAC 
TGGAAGATCG GCTTCCAGCT GTGCAACCAG AACAAATCGG ACTGCTTCTA CCAGACATAC 
TCATCAGGGG TGGATGCGGT GAGGGAGTGG TACCGCTTCC ACTACATCAA CATCCTGTCG 
AGGCTGCCAG AGACTCTGCC ATCCCTGGAG GAGGACACGC TGGGCAACTT CATCTTCGCC 
TGCCGCTTCA ACCAGGTCTC CTGCAACCAG GCGAATTACT CTCACTTCCA CCACCCGATG 
TATGGAAACT GCTATACTTT CAATGACAAG AACAACTCCA ACCTCTGGAT GTCTTCCATG 
CCTGGAATCA ACAACGGTCT GTCCCTGATG CTGCGCGCAG AGCAGAATGA CTTCATTCCC 
CTGCTGTCCA CAGTGACTGG GGCCCGGGTA ATGGTGCACG GGCAGGATGA ACCTGCCTTT 
ATGGATGATG GTGGCTTTAA CTTGCGGCCT GGCGTGGAGA CCTCCATCAG CATGAGGAAG 
GAAACCCTGG ACAGACTTGG GGGCGATTAT GGCGACTGCA CCAAGAATGG CAGTGATGTT 
CCTGTTGAGA ACCTTTACCC TTCAAAGTAC ACACAGCAGG TGTGTATTCA CTCCTGCTTC 
CAGGAGAGCA TGATCAAGGA GTGTGGCTGT GCCTACATCT TCTATCCGCG GCCCCAGAAC 
GTGGAGTACT GTGACTACAG AAAGCACAGT TCCTGGGGGT ACTGCTACTA TAAGCTCCAG 
GTTGACTTCT CCTCAGACCA CCTGGGCTGT TTCACCAAGT GCCGGAAGCC ATGCAGCGTG 
ACCAGCTACC AGCTCTCTGC TGGTTACTCA CGATGGCCCT CGGTGACATC CCAGGAATGG 
GTCTTCCAGA TGCTATCGCG ACAGAACAAT TACACCGTCA ACAACAAGAG AAATGGAGTG 
GCCAAAGTCA ACATCTTCTT CAAGGAGCTG AACTACAAAA CCAATTCTGA GTCTCCCTCT 
GTCACGATGG TCACCCTCCT GTCCAACCTG GGCAGCCAGT GGAGCCTGTG GTTCGGCTCC 
TCGGTGTTGT CTGTGGTGGA GATGGCTGAG CTCGTCTTTG ACCTGCTGGT CATCATGTTC 
CTCATGCTGC TCCGAAGGTT CCGAAGCCGA TACTGGTCTC CAGGCCGAGG GGGCAGGGGT 
GCTCAGGAGG TAGCCTCCAC CCTGGCATCC TCCCCTCCTT CCCACTTCTG CCCCCACCCC 
ATGTCTCTGT CCTTGTCCCA GCCAGGCCCT GCTCCCTCTC CAGCCTTGAC AGCCCCTCCC 
CCTGCCTATG CCACCCTGGG CCCCCGCCCA TCTCCAGGGG GCTCTGCAGG GGCCAGTTCC 
TCCACCTGTC CTCTGGGGGG GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCNN1A ( NM_001038.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_001029.1 Homo sapiens sodium channel epithelial 1 subunit alpha (SCNN1A), transcript variant 1, mRNA.

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CloneID	OHu19374
Clone ID Related Accession (Same CDS sequence)	NM_001038.6 , NM_001038.5
Accession Version	NM_001038.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	amiloride-sensitive sodium channel subunit alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB232484.1 and X76180.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001038.5. Transcript Variant: This variant (1, also known as alpha-ENaC1) represents the predominant transcript, and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X76180.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000228916.7/ ENSP00000228916.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGGGA ACAAGCTGGA GGAGCAGGAC TCTAGCCCTC CACAGTCCAC TCCAGGGCTC 
ATGAAGGGGA ACAAGCGTGA GGAGCAGGGG CTGGGCCCCG AACCTGCGGC GCCCCAGCAG 
CCCACGGCGG AGGAGGAGGC CCTGATCGAG TTCCACCGCT CCTACCGAGA GCTCTTCGAG 
TTCTTCTGCA ACAACACCAC CATCCACGGC GCCATCCGCC TGGTGTGCTC CCAGCACAAC 
CGCATGAAGA CGGCCTTCTG GGCAGTGCTG TGGCTCTGCA CCTTTGGCAT GATGTACTGG 
CAATTCGGCC TGCTTTTCGG AGAGTACTTC AGCTACCCCG TCAGCCTCAA CATCAACCTC 
AACTCGGACA AGCTCGTCTT CCCCGCAGTG ACCATCTGCA CCCTCAATCC CTACAGGTAC 
CCGGAAATTA AAGAGGAGCT GGAGGAGCTG GACCGCATCA CAGAGCAGAC GCTCTTTGAC 
CTGTACAAAT ACAGCTCCTT CACCACTCTC GTGGCCGGCT CCCGCAGCCG TCGCGACCTG 
CGGGGGACTC TGCCGCACCC CTTGCAGCGC CTGAGGGTCC CGCCCCCGCC TCACGGGGCC 
CGTCGAGCCC GTAGCGTGGC CTCCAGCTTG CGGGACAACA ACCCCCAGGT GGACTGGAAG 
GACTGGAAGA TCGGCTTCCA GCTGTGCAAC CAGAACAAAT CGGACTGCTT CTACCAGACA 
TACTCATCAG GGGTGGATGC GGTGAGGGAG TGGTACCGCT TCCACTACAT CAACATCCTG 
TCGAGGCTGC CAGAGACTCT GCCATCCCTG GAGGAGGACA CGCTGGGCAA CTTCATCTTC 
GCCTGCCGCT TCAACCAGGT CTCCTGCAAC CAGGCGAATT ACTCTCACTT CCACCACCCG 
ATGTATGGAA ACTGCTATAC TTTCAATGAC AAGAACAACT CCAACCTCTG GATGTCTTCC 
ATGCCTGGAA TCAACAACGG TCTGTCCCTG ATGCTGCGCG CAGAGCAGAA TGACTTCATT 
CCCCTGCTGT CCACAGTGAC TGGGGCCCGG GTAATGGTGC ACGGGCAGGA TGAACCTGCC 
TTTATGGATG ATGGTGGCTT TAACTTGCGG CCTGGCGTGG AGACCTCCAT CAGCATGAGG 
AAGGAAACCC TGGACAGACT TGGGGGCGAT TATGGCGACT GCACCAAGAA TGGCAGTGAT 
GTTCCTGTTG AGAACCTTTA CCCTTCAAAG TACACACAGC AGGTGTGTAT TCACTCCTGC 
TTCCAGGAGA GCATGATCAA GGAGTGTGGC TGTGCCTACA TCTTCTATCC GCGGCCCCAG 
AACGTGGAGT ACTGTGACTA CAGAAAGCAC AGTTCCTGGG GGTACTGCTA CTATAAGCTC 
CAGGTTGACT TCTCCTCAGA CCACCTGGGC TGTTTCACCA AGTGCCGGAA GCCATGCAGC 
GTGACCAGCT ACCAGCTCTC TGCTGGTTAC TCACGATGGC CCTCGGTGAC ATCCCAGGAA 
TGGGTCTTCC AGATGCTATC GCGACAGAAC AATTACACCG TCAACAACAA GAGAAATGGA 
GTGGCCAAAG TCAACATCTT CTTCAAGGAG CTGAACTACA AAACCAATTC TGAGTCTCCC 
TCTGTCACGA TGGTCACCCT CCTGTCCAAC CTGGGCAGCC AGTGGAGCCT GTGGTTCGGC 
TCCTCGGTGT TGTCTGTGGT GGAGATGGCT GAGCTCGTCT TTGACCTGCT GGTCATCATG 
TTCCTCATGC TGCTCCGAAG GTTCCGAAGC CGATACTGGT CTCCAGGCCG AGGGGGCAGG 
GGTGCTCAGG AGGTAGCCTC CACCCTGGCA TCCTCCCCTC CTTCCCACTT CTGCCCCCAC 
CCCATGTCTC TGTCCTTGTC CCAGCCAGGC CCTGCTCCCT CTCCAGCCTT GACAGCCCCT 
CCCCCTGCCT ATGCCACCCT GGGCCCCCGC CCATCTCCAG GGGGCTCTGC AGGGGCCAGT 
TCCTCCACCT GTCCTCTGGG GGGGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029.1
CDS	114..2123
Translation	MEGNKLEEQDSSPPQSTPGLMKGNKREEQGLGPEPAAPQQPTAEEEALIEFHRSYRELFEFFCNNTTIHGAIRLVCSQHNRMKTAFWAVLWLCTFGMMYWQFGLLFGEYFSYPVSLNINLNSDKLVFPAVTICTLNPYRYPEIKEELEELDRITEQTLFDLYKYSSFTTLVAGSRSRRDLRGTLPHPLQRLRVPPPPHGARRARSVASSLRDNNPQVDWKDWKIGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYINILSRLPETLPSLEEDTLGNFIFACRFNQVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAEQNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLYPSKYTQQVCIHSCFQESMIKECGCAYIFYPRPQNVEYCDYRKHSSWGYCYYKLQVDFSSDHLGCFTKCRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQNNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVFDLLVIMFLMLLRRFRSRYWSPGRGGRGAQEVASTLASSPPSHFCPHPMSLSLSQPGPAPSPALTAPPPAYATLGPRPSPGGSAGASSSTCPLGGP

Target ORF information:

RefSeq Version	NM_001038.6
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium channel epithelial 1 subunit alpha (SCNN1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038.6

ORF Insert Sequence:

ATGGAGGGGA ACAAGCTGGA GGAGCAGGAC TCTAGCCCTC CACAGTCCAC TCCAGGGCTC 
ATGAAGGGGA ACAAGCGTGA GGAGCAGGGG CTGGGCCCCG AACCTGCGGC GCCCCAGCAG 
CCCACGGCGG AGGAGGAGGC CCTGATCGAG TTCCACCGCT CCTACCGAGA GCTCTTCGAG 
TTCTTCTGCA ACAACACCAC CATCCACGGC GCCATCCGCC TGGTGTGCTC CCAGCACAAC 
CGCATGAAGA CGGCCTTCTG GGCAGTGCTG TGGCTCTGCA CCTTTGGCAT GATGTACTGG 
CAATTCGGCC TGCTTTTCGG AGAGTACTTC AGCTACCCCG TCAGCCTCAA CATCAACCTC 
AACTCGGACA AGCTCGTCTT CCCCGCAGTG ACCATCTGCA CCCTCAATCC CTACAGGTAC 
CCGGAAATTA AAGAGGAGCT GGAGGAGCTG GACCGCATCA CAGAGCAGAC GCTCTTTGAC 
CTGTACAAAT ACAGCTCCTT CACCACTCTC GTGGCCGGCT CCCGCAGCCG TCGCGACCTG 
CGGGGGACTC TGCCGCACCC CTTGCAGCGC CTGAGGGTCC CGCCCCCGCC TCACGGGGCC 
CGTCGAGCCC GTAGCGTGGC CTCCAGCTTG CGGGACAACA ACCCCCAGGT GGACTGGAAG 
GACTGGAAGA TCGGCTTCCA GCTGTGCAAC CAGAACAAAT CGGACTGCTT CTACCAGACA 
TACTCATCAG GGGTGGATGC GGTGAGGGAG TGGTACCGCT TCCACTACAT CAACATCCTG 
TCGAGGCTGC CAGAGACTCT GCCATCCCTG GAGGAGGACA CGCTGGGCAA CTTCATCTTC 
GCCTGCCGCT TCAACCAGGT CTCCTGCAAC CAGGCGAATT ACTCTCACTT CCACCACCCG 
ATGTATGGAA ACTGCTATAC TTTCAATGAC AAGAACAACT CCAACCTCTG GATGTCTTCC 
ATGCCTGGAA TCAACAACGG TCTGTCCCTG ATGCTGCGCG CAGAGCAGAA TGACTTCATT 
CCCCTGCTGT CCACAGTGAC TGGGGCCCGG GTAATGGTGC ACGGGCAGGA TGAACCTGCC 
TTTATGGATG ATGGTGGCTT TAACTTGCGG CCTGGCGTGG AGACCTCCAT CAGCATGAGG 
AAGGAAACCC TGGACAGACT TGGGGGCGAT TATGGCGACT GCACCAAGAA TGGCAGTGAT 
GTTCCTGTTG AGAACCTTTA CCCTTCAAAG TACACACAGC AGGTGTGTAT TCACTCCTGC 
TTCCAGGAGA GCATGATCAA GGAGTGTGGC TGTGCCTACA TCTTCTATCC GCGGCCCCAG 
AACGTGGAGT ACTGTGACTA CAGAAAGCAC AGTTCCTGGG GGTACTGCTA CTATAAGCTC 
CAGGTTGACT TCTCCTCAGA CCACCTGGGC TGTTTCACCA AGTGCCGGAA GCCATGCAGC 
GTGACCAGCT ACCAGCTCTC TGCTGGTTAC TCACGATGGC CCTCGGTGAC ATCCCAGGAA 
TGGGTCTTCC AGATGCTATC GCGACAGAAC AATTACACCG TCAACAACAA GAGAAATGGA 
GTGGCCAAAG TCAACATCTT CTTCAAGGAG CTGAACTACA AAACCAATTC TGAGTCTCCC 
TCTGTCACGA TGGTCACCCT CCTGTCCAAC CTGGGCAGCC AGTGGAGCCT GTGGTTCGGC 
TCCTCGGTGT TGTCTGTGGT GGAGATGGCT GAGCTCGTCT TTGACCTGCT GGTCATCATG 
TTCCTCATGC TGCTCCGAAG GTTCCGAAGC CGATACTGGT CTCCAGGCCG AGGGGGCAGG 
GGTGCTCAGG AGGTAGCCTC CACCCTGGCA TCCTCCCCTC CTTCCCACTT CTGCCCCCAC 
CCCATGTCTC TGTCCTTGTC CCAGCCAGGC CCTGCTCCCT CTCCAGCCTT GACAGCCCCT 
CCCCCTGCCT ATGCCACCCT GGGCCCCCGC CCATCTCCAG GGGGCTCTGC AGGGGCCAGT 
TCCTCCACCT GTCCTCTGGG GGGGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCNN1A ( NM_001038.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001029.1 Homo sapiens sodium channel epithelial 1 alpha subunit (SCNN1A), transcript variant 1, mRNA.

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CloneID	OHu19374
Clone ID Related Accession (Same CDS sequence)	NM_001038.6 , NM_001038.5
Accession Version	NM_001038.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	amiloride-sensitive sodium channel subunit alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB232484.1, X76180.1 and BU680600.1. This sequence is a reference standard in the RefSeqGene project. On Apr 23, 2009 this sequence version replaced NM_001038.4. Transcript Variant: This variant (1, also known as alpha-ENaC1) represents the predominant transcript, and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X76180.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGGA ACAAGCTGGA GGAGCAGGAC TCTAGCCCTC CACAGTCCAC TCCAGGGCTC 
ATGAAGGGGA ACAAGCGTGA GGAGCAGGGG CTGGGCCCCG AACCTGCGGC GCCCCAGCAG 
CCCACGGCGG AGGAGGAGGC CCTGATCGAG TTCCACCGCT CCTACCGAGA GCTCTTCGAG 
TTCTTCTGCA ACAACACCAC CATCCACGGC GCCATCCGCC TGGTGTGCTC CCAGCACAAC 
CGCATGAAGA CGGCCTTCTG GGCAGTGCTG TGGCTCTGCA CCTTTGGCAT GATGTACTGG 
CAATTCGGCC TGCTTTTCGG AGAGTACTTC AGCTACCCCG TCAGCCTCAA CATCAACCTC 
AACTCGGACA AGCTCGTCTT CCCCGCAGTG ACCATCTGCA CCCTCAATCC CTACAGGTAC 
CCGGAAATTA AAGAGGAGCT GGAGGAGCTG GACCGCATCA CAGAGCAGAC GCTCTTTGAC 
CTGTACAAAT ACAGCTCCTT CACCACTCTC GTGGCCGGCT CCCGCAGCCG TCGCGACCTG 
CGGGGGACTC TGCCGCACCC CTTGCAGCGC CTGAGGGTCC CGCCCCCGCC TCACGGGGCC 
CGTCGAGCCC GTAGCGTGGC CTCCAGCTTG CGGGACAACA ACCCCCAGGT GGACTGGAAG 
GACTGGAAGA TCGGCTTCCA GCTGTGCAAC CAGAACAAAT CGGACTGCTT CTACCAGACA 
TACTCATCAG GGGTGGATGC GGTGAGGGAG TGGTACCGCT TCCACTACAT CAACATCCTG 
TCGAGGCTGC CAGAGACTCT GCCATCCCTG GAGGAGGACA CGCTGGGCAA CTTCATCTTC 
GCCTGCCGCT TCAACCAGGT CTCCTGCAAC CAGGCGAATT ACTCTCACTT CCACCACCCG 
ATGTATGGAA ACTGCTATAC TTTCAATGAC AAGAACAACT CCAACCTCTG GATGTCTTCC 
ATGCCTGGAA TCAACAACGG TCTGTCCCTG ATGCTGCGCG CAGAGCAGAA TGACTTCATT 
CCCCTGCTGT CCACAGTGAC TGGGGCCCGG GTAATGGTGC ACGGGCAGGA TGAACCTGCC 
TTTATGGATG ATGGTGGCTT TAACTTGCGG CCTGGCGTGG AGACCTCCAT CAGCATGAGG 
AAGGAAACCC TGGACAGACT TGGGGGCGAT TATGGCGACT GCACCAAGAA TGGCAGTGAT 
GTTCCTGTTG AGAACCTTTA CCCTTCAAAG TACACACAGC AGGTGTGTAT TCACTCCTGC 
TTCCAGGAGA GCATGATCAA GGAGTGTGGC TGTGCCTACA TCTTCTATCC GCGGCCCCAG 
AACGTGGAGT ACTGTGACTA CAGAAAGCAC AGTTCCTGGG GGTACTGCTA CTATAAGCTC 
CAGGTTGACT TCTCCTCAGA CCACCTGGGC TGTTTCACCA AGTGCCGGAA GCCATGCAGC 
GTGACCAGCT ACCAGCTCTC TGCTGGTTAC TCACGATGGC CCTCGGTGAC ATCCCAGGAA 
TGGGTCTTCC AGATGCTATC GCGACAGAAC AATTACACCG TCAACAACAA GAGAAATGGA 
GTGGCCAAAG TCAACATCTT CTTCAAGGAG CTGAACTACA AAACCAATTC TGAGTCTCCC 
TCTGTCACGA TGGTCACCCT CCTGTCCAAC CTGGGCAGCC AGTGGAGCCT GTGGTTCGGC 
TCCTCGGTGT TGTCTGTGGT GGAGATGGCT GAGCTCGTCT TTGACCTGCT GGTCATCATG 
TTCCTCATGC TGCTCCGAAG GTTCCGAAGC CGATACTGGT CTCCAGGCCG AGGGGGCAGG 
GGTGCTCAGG AGGTAGCCTC CACCCTGGCA TCCTCCCCTC CTTCCCACTT CTGCCCCCAC 
CCCATGTCTC TGTCCTTGTC CCAGCCAGGC CCTGCTCCCT CTCCAGCCTT GACAGCCCCT 
CCCCCTGCCT ATGCCACCCT GGGCCCCCGC CCATCTCCAG GGGGCTCTGC AGGGGCCAGT 
TCCTCCACCT GTCCTCTGGG GGGGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029.1
CDS	290..2299
Translation	MEGNKLEEQDSSPPQSTPGLMKGNKREEQGLGPEPAAPQQPTAEEEALIEFHRSYRELFEFFCNNTTIHGAIRLVCSQHNRMKTAFWAVLWLCTFGMMYWQFGLLFGEYFSYPVSLNINLNSDKLVFPAVTICTLNPYRYPEIKEELEELDRITEQTLFDLYKYSSFTTLVAGSRSRRDLRGTLPHPLQRLRVPPPPHGARRARSVASSLRDNNPQVDWKDWKIGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYINILSRLPETLPSLEEDTLGNFIFACRFNQVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAEQNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLYPSKYTQQVCIHSCFQESMIKECGCAYIFYPRPQNVEYCDYRKHSSWGYCYYKLQVDFSSDHLGCFTKCRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQNNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVFDLLVIMFLMLLRRFRSRYWSPGRGGRGAQEVASTLASSPPSHFCPHPMSLSLSQPGPAPSPALTAPPPAYATLGPRPSPGGSAGASSSTCPLGGP

Target ORF information:

RefSeq Version	NM_001038.5
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium channel epithelial 1 alpha subunit (SCNN1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038.5

ORF Insert Sequence:

ATGGAGGGGA ACAAGCTGGA GGAGCAGGAC TCTAGCCCTC CACAGTCCAC TCCAGGGCTC 
ATGAAGGGGA ACAAGCGTGA GGAGCAGGGG CTGGGCCCCG AACCTGCGGC GCCCCAGCAG 
CCCACGGCGG AGGAGGAGGC CCTGATCGAG TTCCACCGCT CCTACCGAGA GCTCTTCGAG 
TTCTTCTGCA ACAACACCAC CATCCACGGC GCCATCCGCC TGGTGTGCTC CCAGCACAAC 
CGCATGAAGA CGGCCTTCTG GGCAGTGCTG TGGCTCTGCA CCTTTGGCAT GATGTACTGG 
CAATTCGGCC TGCTTTTCGG AGAGTACTTC AGCTACCCCG TCAGCCTCAA CATCAACCTC 
AACTCGGACA AGCTCGTCTT CCCCGCAGTG ACCATCTGCA CCCTCAATCC CTACAGGTAC 
CCGGAAATTA AAGAGGAGCT GGAGGAGCTG GACCGCATCA CAGAGCAGAC GCTCTTTGAC 
CTGTACAAAT ACAGCTCCTT CACCACTCTC GTGGCCGGCT CCCGCAGCCG TCGCGACCTG 
CGGGGGACTC TGCCGCACCC CTTGCAGCGC CTGAGGGTCC CGCCCCCGCC TCACGGGGCC 
CGTCGAGCCC GTAGCGTGGC CTCCAGCTTG CGGGACAACA ACCCCCAGGT GGACTGGAAG 
GACTGGAAGA TCGGCTTCCA GCTGTGCAAC CAGAACAAAT CGGACTGCTT CTACCAGACA 
TACTCATCAG GGGTGGATGC GGTGAGGGAG TGGTACCGCT TCCACTACAT CAACATCCTG 
TCGAGGCTGC CAGAGACTCT GCCATCCCTG GAGGAGGACA CGCTGGGCAA CTTCATCTTC 
GCCTGCCGCT TCAACCAGGT CTCCTGCAAC CAGGCGAATT ACTCTCACTT CCACCACCCG 
ATGTATGGAA ACTGCTATAC TTTCAATGAC AAGAACAACT CCAACCTCTG GATGTCTTCC 
ATGCCTGGAA TCAACAACGG TCTGTCCCTG ATGCTGCGCG CAGAGCAGAA TGACTTCATT 
CCCCTGCTGT CCACAGTGAC TGGGGCCCGG GTAATGGTGC ACGGGCAGGA TGAACCTGCC 
TTTATGGATG ATGGTGGCTT TAACTTGCGG CCTGGCGTGG AGACCTCCAT CAGCATGAGG 
AAGGAAACCC TGGACAGACT TGGGGGCGAT TATGGCGACT GCACCAAGAA TGGCAGTGAT 
GTTCCTGTTG AGAACCTTTA CCCTTCAAAG TACACACAGC AGGTGTGTAT TCACTCCTGC 
TTCCAGGAGA GCATGATCAA GGAGTGTGGC TGTGCCTACA TCTTCTATCC GCGGCCCCAG 
AACGTGGAGT ACTGTGACTA CAGAAAGCAC AGTTCCTGGG GGTACTGCTA CTATAAGCTC 
CAGGTTGACT TCTCCTCAGA CCACCTGGGC TGTTTCACCA AGTGCCGGAA GCCATGCAGC 
GTGACCAGCT ACCAGCTCTC TGCTGGTTAC TCACGATGGC CCTCGGTGAC ATCCCAGGAA 
TGGGTCTTCC AGATGCTATC GCGACAGAAC AATTACACCG TCAACAACAA GAGAAATGGA 
GTGGCCAAAG TCAACATCTT CTTCAAGGAG CTGAACTACA AAACCAATTC TGAGTCTCCC 
TCTGTCACGA TGGTCACCCT CCTGTCCAAC CTGGGCAGCC AGTGGAGCCT GTGGTTCGGC 
TCCTCGGTGT TGTCTGTGGT GGAGATGGCT GAGCTCGTCT TTGACCTGCT GGTCATCATG 
TTCCTCATGC TGCTCCGAAG GTTCCGAAGC CGATACTGGT CTCCAGGCCG AGGGGGCAGG 
GGTGCTCAGG AGGTAGCCTC CACCCTGGCA TCCTCCCCTC CTTCCCACTT CTGCCCCCAC 
CCCATGTCTC TGTCCTTGTC CCAGCCAGGC CCTGCTCCCT CTCCAGCCTT GACAGCCCCT 
CCCCCTGCCT ATGCCACCCT GGGCCCCCGC CCATCTCCAG GGGGCTCTGC AGGGGCCAGT 
TCCTCCACCT GTCCTCTGGG GGGGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCNN1A ( NM_001159575.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153047.1 Homo sapiens sodium channel epithelial 1 subunit alpha (SCNN1A), transcript variant 3, mRNA.

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CloneID	OHu14485
Clone ID Related Accession (Same CDS sequence)	NM_001159575.1
Accession Version	NM_001159575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	amiloride-sensitive sodium channel subunit alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK304379.1, X76180.1 and BU680600.1. Transcript Variant: This variant (3) contains an alternate exon in the 5' coding region, and uses an in-frame upstream start codon compared to variant 1. This results in an isoform (3) with a longer N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304379.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGTA TCAAGGGGAA CAAGCTGGAG GAGCAGGACC CTAGACCTCT GCAGCCCATA 
CCAGGTCTCA TGGAGGGGAA CAAGCTGGAG GAGCAGGACT CTAGCCCTCC ACAGTCCACT 
CCAGGGCTCA TGAAGGGGAA CAAGCGTGAG GAGCAGGGGC TGGGCCCCGA ACCTGCGGCG 
CCCCAGCAGC CCACGGCGGA GGAGGAGGCC CTGATCGAGT TCCACCGCTC CTACCGAGAG 
CTCTTCGAGT TCTTCTGCAA CAACACCACC ATCCACGGCG CCATCCGCCT GGTGTGCTCC 
CAGCACAACC GCATGAAGAC GGCCTTCTGG GCAGTGCTGT GGCTCTGCAC CTTTGGCATG 
ATGTACTGGC AATTCGGCCT GCTTTTCGGA GAGTACTTCA GCTACCCCGT CAGCCTCAAC 
ATCAACCTCA ACTCGGACAA GCTCGTCTTC CCCGCAGTGA CCATCTGCAC CCTCAATCCC 
TACAGGTACC CGGAAATTAA AGAGGAGCTG GAGGAGCTGG ACCGCATCAC AGAGCAGACG 
CTCTTTGACC TGTACAAATA CAGCTCCTTC ACCACTCTCG TGGCCGGCTC CCGCAGCCGT 
CGCGACCTGC GGGGGACTCT GCCGCACCCC TTGCAGCGCC TGAGGGTCCC GCCCCCGCCT 
CACGGGGCCC GTCGAGCCCG TAGCGTGGCC TCCAGCTTGC GGGACAACAA CCCCCAGGTG 
GACTGGAAGG ACTGGAAGAT CGGCTTCCAG CTGTGCAACC AGAACAAATC GGACTGCTTC 
TACCAGACAT ACTCATCAGG GGTGGATGCG GTGAGGGAGT GGTACCGCTT CCACTACATC 
AACATCCTGT CGAGGCTGCC AGAGACTCTG CCATCCCTGG AGGAGGACAC GCTGGGCAAC 
TTCATCTTCG CCTGCCGCTT CAACCAGGTC TCCTGCAACC AGGCGAATTA CTCTCACTTC 
CACCACCCGA TGTATGGAAA CTGCTATACT TTCAATGACA AGAACAACTC CAACCTCTGG 
ATGTCTTCCA TGCCTGGAAT CAACAACGGT CTGTCCCTGA TGCTGCGCGC AGAGCAGAAT 
GACTTCATTC CCCTGCTGTC CACAGTGACT GGGGCCCGGG TAATGGTGCA CGGGCAGGAT 
GAACCTGCCT TTATGGATGA TGGTGGCTTT AACTTGCGGC CTGGCGTGGA GACCTCCATC 
AGCATGAGGA AGGAAACCCT GGACAGACTT GGGGGCGATT ATGGCGACTG CACCAAGAAT 
GGCAGTGATG TTCCTGTTGA GAACCTTTAC CCTTCAAAGT ACACACAGCA GGTGTGTATT 
CACTCCTGCT TCCAGGAGAG CATGATCAAG GAGTGTGGCT GTGCCTACAT CTTCTATCCG 
CGGCCCCAGA ACGTGGAGTA CTGTGACTAC AGAAAGCACA GTTCCTGGGG GTACTGCTAC 
TATAAGCTCC AGGTTGACTT CTCCTCAGAC CACCTGGGCT GTTTCACCAA GTGCCGGAAG 
CCATGCAGCG TGACCAGCTA CCAGCTCTCT GCTGGTTACT CACGATGGCC CTCGGTGACA 
TCCCAGGAAT GGGTCTTCCA GATGCTATCG CGACAGAACA ATTACACCGT CAACAACAAG 
AGAAATGGAG TGGCCAAAGT CAACATCTTC TTCAAGGAGC TGAACTACAA AACCAATTCT 
GAGTCTCCCT CTGTCACGAT GGTCACCCTC CTGTCCAACC TGGGCAGCCA GTGGAGCCTG 
TGGTTCGGCT CCTCGGTGTT GTCTGTGGTG GAGATGGCTG AGCTCGTCTT TGACCTGCTG 
GTCATCATGT TCCTCATGCT GCTCCGAAGG TTCCGAAGCC GATACTGGTC TCCAGGCCGA 
GGGGGCAGGG GTGCTCAGGA GGTAGCCTCC ACCCTGGCAT CCTCCCCTCC TTCCCACTTC 
TGCCCCCACC CCATGTCTCT GTCCTTGTCC CAGCCAGGCC CTGCTCCCTC TCCAGCCTTG 
ACAGCCCCTC CCCCTGCCTA TGCCACCCTG GGCCCCCGCC CATCTCCAGG GGGCTCTGCA 
GGGGCCAGTT CCTCCACCTG TCCTCTGGGG GGGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153047.1
CDS	92..2170
Translation	MSSIKGNKLEEQDPRPLQPIPGLMEGNKLEEQDSSPPQSTPGLMKGNKREEQGLGPEPAAPQQPTAEEEALIEFHRSYRELFEFFCNNTTIHGAIRLVCSQHNRMKTAFWAVLWLCTFGMMYWQFGLLFGEYFSYPVSLNINLNSDKLVFPAVTICTLNPYRYPEIKEELEELDRITEQTLFDLYKYSSFTTLVAGSRSRRDLRGTLPHPLQRLRVPPPPHGARRARSVASSLRDNNPQVDWKDWKIGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYINILSRLPETLPSLEEDTLGNFIFACRFNQVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAEQNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLYPSKYTQQVCIHSCFQESMIKECGCAYIFYPRPQNVEYCDYRKHSSWGYCYYKLQVDFSSDHLGCFTKCRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQNNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVFDLLVIMFLMLLRRFRSRYWSPGRGGRGAQEVASTLASSPPSHFCPHPMSLSLSQPGPAPSPALTAPPPAYATLGPRPSPGGSAGASSSTCPLGGP

Target ORF information:

RefSeq Version	NM_001159575.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium channel epithelial 1 subunit alpha (SCNN1A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159575.1

ORF Insert Sequence:

ATGAGCAGTA TCAAGGGGAA CAAGCTGGAG GAGCAGGACC CTAGACCTCT GCAGCCCATA 
CCAGGTCTCA TGGAGGGGAA CAAGCTGGAG GAGCAGGACT CTAGCCCTCC ACAGTCCACT 
CCAGGGCTCA TGAAGGGGAA CAAGCGTGAG GAGCAGGGGC TGGGCCCCGA ACCTGCGGCG 
CCCCAGCAGC CCACGGCGGA GGAGGAGGCC CTGATCGAGT TCCACCGCTC CTACCGAGAG 
CTCTTCGAGT TCTTCTGCAA CAACACCACC ATCCACGGCG CCATCCGCCT GGTGTGCTCC 
CAGCACAACC GCATGAAGAC GGCCTTCTGG GCAGTGCTGT GGCTCTGCAC CTTTGGCATG 
ATGTACTGGC AATTCGGCCT GCTTTTCGGA GAGTACTTCA GCTACCCCGT CAGCCTCAAC 
ATCAACCTCA ACTCGGACAA GCTCGTCTTC CCCGCAGTGA CCATCTGCAC CCTCAATCCC 
TACAGGTACC CGGAAATTAA AGAGGAGCTG GAGGAGCTGG ACCGCATCAC AGAGCAGACG 
CTCTTTGACC TGTACAAATA CAGCTCCTTC ACCACTCTCG TGGCCGGCTC CCGCAGCCGT 
CGCGACCTGC GGGGGACTCT GCCGCACCCC TTGCAGCGCC TGAGGGTCCC GCCCCCGCCT 
CACGGGGCCC GTCGAGCCCG TAGCGTGGCC TCCAGCTTGC GGGACAACAA CCCCCAGGTG 
GACTGGAAGG ACTGGAAGAT CGGCTTCCAG CTGTGCAACC AGAACAAATC GGACTGCTTC 
TACCAGACAT ACTCATCAGG GGTGGATGCG GTGAGGGAGT GGTACCGCTT CCACTACATC 
AACATCCTGT CGAGGCTGCC AGAGACTCTG CCATCCCTGG AGGAGGACAC GCTGGGCAAC 
TTCATCTTCG CCTGCCGCTT CAACCAGGTC TCCTGCAACC AGGCGAATTA CTCTCACTTC 
CACCACCCGA TGTATGGAAA CTGCTATACT TTCAATGACA AGAACAACTC CAACCTCTGG 
ATGTCTTCCA TGCCTGGAAT CAACAACGGT CTGTCCCTGA TGCTGCGCGC AGAGCAGAAT 
GACTTCATTC CCCTGCTGTC CACAGTGACT GGGGCCCGGG TAATGGTGCA CGGGCAGGAT 
GAACCTGCCT TTATGGATGA TGGTGGCTTT AACTTGCGGC CTGGCGTGGA GACCTCCATC 
AGCATGAGGA AGGAAACCCT GGACAGACTT GGGGGCGATT ATGGCGACTG CACCAAGAAT 
GGCAGTGATG TTCCTGTTGA GAACCTTTAC CCTTCAAAGT ACACACAGCA GGTGTGTATT 
CACTCCTGCT TCCAGGAGAG CATGATCAAG GAGTGTGGCT GTGCCTACAT CTTCTATCCG 
CGGCCCCAGA ACGTGGAGTA CTGTGACTAC AGAAAGCACA GTTCCTGGGG GTACTGCTAC 
TATAAGCTCC AGGTTGACTT CTCCTCAGAC CACCTGGGCT GTTTCACCAA GTGCCGGAAG 
CCATGCAGCG TGACCAGCTA CCAGCTCTCT GCTGGTTACT CACGATGGCC CTCGGTGACA 
TCCCAGGAAT GGGTCTTCCA GATGCTATCG CGACAGAACA ATTACACCGT CAACAACAAG 
AGAAATGGAG TGGCCAAAGT CAACATCTTC TTCAAGGAGC TGAACTACAA AACCAATTCT 
GAGTCTCCCT CTGTCACGAT GGTCACCCTC CTGTCCAACC TGGGCAGCCA GTGGAGCCTG 
TGGTTCGGCT CCTCGGTGTT GTCTGTGGTG GAGATGGCTG AGCTCGTCTT TGACCTGCTG 
GTCATCATGT TCCTCATGCT GCTCCGAAGG TTCCGAAGCC GATACTGGTC TCCAGGCCGA 
GGGGGCAGGG GTGCTCAGGA GGTAGCCTCC ACCCTGGCAT CCTCCCCTCC TTCCCACTTC 
TGCCCCCACC CCATGTCTCT GTCCTTGTCC CAGCCAGGCC CTGCTCCCTC TCCAGCCTTG 
ACAGCCCCTC CCCCTGCCTA TGCCACCCTG GGCCCCCGCC CATCTCCAGG GGGCTCTGCA 
GGGGCCAGTT CCTCCACCTG TCCTCTGGGG GGGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mapping allosteric linkage to channel gating of extracellular domains in the human epithelial sodium channel.
The Journal of biological chemistry(2018 Jan)
Shobair M,Popov KI,Dang YL,He H,Stutts MJ,Dokholyan NV

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SCNN1A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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