ATXN7 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATXN7
Entrez Gene ID	6314
Full Name	ataxin 7
Synonyms	ADCAII,OPCA3,SCA7
General protein information	Preferred Names ataxin 7 Names ataxin-7 spinocerebellar ataxia type 7 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p14.1
Summary	The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the 'pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. This locus has been mapped to chromosome 3, and it has been determined that the diseased allele associated with spinocerebellar ataxia-7 contains 37-306 CAG repeats (near the N-terminus), compared to 4-35 in the normal allele. The encoded protein is a component of the SPT3/TAF9/GCN5 acetyltransferase (STAGA) and TBP-free TAF-containing (TFTC) chromatin remodeling complexes, and it thus plays a role in transcriptional regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016].
Disorder MIM:	607640
Disorder Html:	Spinocerebellar ataxia 7, 164500 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128149.2	NP_001121621.2	ataxin-7 isoform c
NM_001128149.3	NP_001121621.2	ataxin-7 isoform c
NM_000333.3	NP_000324.1	ataxin-7 isoform a
NM_001177387.1	NP_001170858.1	ataxin-7 isoform b

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100001490	XP_001341475.5
Xenopus tropicalis (tropical clawed frog)	atxn7	NP_001106501.1
Homo sapiens (human)	ATXN7	NP_001170858.1
Pan troglodytes (chimpanzee)	ATXN7	XP_001174619.2
Rattus norvegicus (Norway rat)	Atxn7	XP_006251790.1
Canis lupus familiaris (dog)	ATXN7	XP_005632413.1
Bos taurus (cattle)	ATXN7	XP_002697032.2
Mus musculus (house mouse)	Atxn7	NP_631973.3
Gallus gallus (chicken)	ATXN7	XP_004944715.1

Gene Ontology
Bibliography

Related articles in PubMed

A Complete Association of an intronic SNP rs6798742 with Origin of Spinocerebellar Ataxia Type 7-CAG Expansion Loci in the Indian and Mexican Population.
Faruq M, Maga?a JJ, Suroliya V, Narang A, Murillo-Melo NM, Hern?ndez-Hern?ndez O, Srivastava AK, Mukerji M
Annals of human genetics81(5)197-204(2017 Sep)

Spinocerebellar Ataxia.
Wentz S, Jusufbegovic D
Ophthalmology124(7)1071(2017 Jul)

Large normal-range TBP and ATXN7 CAG repeat lengths are associated with increased lifetime risk of depression.
Gardiner SL, van Belzen MJ, Boogaard MW, van Roon-Mom WMC, Rozing MP, van Hemert AM, Smit JH, Beekman ATF, van Grootheest G, Schoevers RA, Oude Voshaar RC, Comijs HC, Penninx BWJH, van der Mast RC, Roos RAC, Aziz NA
Translational psychiatry7(6)e1143(2017 Jun)

ATXN7 Gene Variants and Expression Predict Post-Operative Clinical Outcomes in Hepatitis B Virus-Related Hepatocellular Carcinoma.
Han C, Yu L, Liu X, Yu T, Qin W, Liao X, Liu Z, Lu S, Chen Z, Su H, Zhu G, Qin X, Gui Y, Li J, Xiao K, Chen X, Ye X, Peng M, Dong J, Peng T
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology39(6)2427-2438(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The intronic SNP rs6798742 is associated with ATXN7 CAG-region expansion.
Title: A Complete Association of an intronic SNP rs6798742 with Origin of Spinocerebellar Ataxia Type 7-CAG Expansion Loci in the Indian and Mexican Population.

Results identified a chromosomal translocation between Rad51C and Ataxin-7 in colorectal tumors. The in-frame fusion transcript results in a fusion protein with molecular weight of 110 KDa. In vitro 5-Azacytidine treatment of colorectal tumor cells showed expression of the fusion gene is regulated by promoter methylation.
Title: Rad51C-ATXN7 fusion gene expression in colorectal tumors.

ATXN7 may be a potential predictor of post-operative prognosis of Hepatitis B Virus-related hepatocellular carcinoma .
Title: ATXN7 Gene Variants and Expression Predict Post-Operative Clinical Outcomes in Hepatitis B Virus-Related Hepatocellular Carcinoma.

Data show that the aggregates formed by polyQ-expanded ataxin 7 sequester ubiquitin-specific protease (USP22) through specific interactions.
Title: Aggregation of Polyglutamine-expanded Ataxin 7 Protein Specifically Sequesters Ubiquitin-specific Protease 22 and Deteriorates Its Deubiquitinating Function in the Spt-Ada-Gcn5-Acetyltransferase (SAGA) Complex.

Two pathological polyglutamine proteins, truncated Ataxin-7 and full-length Ataxin-3, suggest that accumulation of insoluble aggregates beyond a critical threshold could be responsible for neurotoxicity.
Title: Quantification of Ataxin-3 and Ataxin-7 aggregates formed in?vivo in Drosophila reveals a threshold of aggregated polyglutamine proteins associated with cellular toxicity.

The following ATXN7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATXN7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21671	NM_001177387.1 Latest version!	Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu20360	NM_001128149.3 Latest version!	Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7c, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
OHu26052	NM_000333.3 Latest version!	Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu20360	NM_001128149.2	Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7c, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATXN7 ( NM_001177387.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001170858.1 Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7b, mRNA.

Price & Availability↑

CloneID	OHu21671
Clone ID Related Accession (Same CDS sequence)	NM_001177387.1
Accession Version	NM_001177387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	ataxin-7 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ000517.1, AA398030.1, AC012557.23 and BI494079.1. Transcript Variant: This variant (SCA7b) includes an alternate exon that causes a frameshift in the 3' coding region, compared to variant SCA7a, resulting in an isoform (b) with a distinct and longer C-terminus, compared to isoform a. The 5' UTR is incomplete in this variant due to the presence of alternate splicing choices further upstream. There are no publicly available full-length transcripts representing this variant; it is represented based on data in PMID:12533095. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGC GGGCCGCGGA TGACGTCAGG GGGGAGCCGC GCCGCGCGGC GGCGGCGGCG 
GGCGGAGCAG CGGCCGCGGC CGCCCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCG 
CCGCCTCCGC AGCCCCAGCG GCAGCAGCAC CCGCCACCGC CGCCACGGCG CACACGGCCG 
GAGGACGGCG GGCCCGGCGC CGCCTCCACC TCGGCCGCCG CAATGGCGAC GGTCGGGGAG 
CGCAGGCCTC TGCCCAGTCC TGAAGTGATG CTGGGACAGT CGTGGAATCT GTGGGTTGAG 
GCTTCCAAAC TTCCTGGGAA GGACGGGACA GAATTGGACG AAAGTTTCAA GGAGTTTGGG 
AAAAACCGCG AAGTCATGGG GCTCTGTCGG GAAGACATGC CAATATTTGG TTTCTGTCCA 
GCCCATGATG ATTTCTACTT GGTGGTGTGT AACGACTGTA ATCAGGTTGT CAAACCGCAG 
GCATTTCAAT CACATTATGA AAGAAGACAT AGCTCATCCA GCAAGCCGCC TTTGGCCGTT 
CCTCCCACTT CAGTATTTTC CTTCTTCCCT TCTCTGTCCA AAAGCAAAGG AGGCAGTGCA 
AGTGGAAGCA ACCGTTCTTC CAGTGGAGGT GTTCTTAGCG CATCCTCATC AAGTTCCAAG 
TTGTTGAAAT CACCCAAAGA GAAACTGCAG CTCAGGGGGA ACACCAGGCC AATGCATCCC 
ATTCAGCAAA GTAGAGTTCC CCATGGTAGA ATCATGACAC CCTCTGTGAA AGTGGAAAAG 
ATTCATCCGA AAATGGATGG CACACTACTG AAATCTGCGG TGGGGCCAAC CTGTCCTGCT 
ACTGTGAGTT CCTTAGTCAA GCCTGGCCTT AACTGCCCCT CAATACCAAA GCCAACCTTG 
CCTTCACCTG GACAGATTCT GAATGGCAAA GGGCTTCCTG CACCGCCCAC TCTGGAAAAG 
AAACCTGAAG ACAATTCCAA TAATAGGAAA TTTTTAAATA AGAGATTATC AGAAAGAGAG 
TTTGATCCTG ACATCCACTG TGGGGTTATT GATCTCGACA CCAAGAAGCC CTGCACCCGG 
TCTTTGACAT GCAAGACACA TTCCTTAACC CAGCGCAGGG CTGTCCAGGG TAGAAGAAAA 
CGATTTGATG TGTTATTAGC CGAGCACAAA AACAAAACCA GGGAAAAGGA ATTGATTCGC 
CATCCGGACT CTCAGCAACC ACCGCAGCCT CTCAGGGACC CGCATCCCGC CCCTCCTAGA 
ACGTCACAGG AGCCGCACCA AAACCCTCAC GGAGTGATTC CTTCCGAATC AAAGCCTTTT 
GTAGCTAGTA AACCTAAACC TCACACCCCC AGTCTTCCAA GGCCTCCAGG CTGCCCTGCT 
CAGCAAGGTG GGAGTGCCCC CATTGACCCT CCTCCAGTCC ATGAATCTCC ACACCCTCCC 
CTGCCTGCCA CTGAGCCAGC TTCTCGGTTA TCCAGTGAGG AGGGCGAAGG CGATGACAAA 
GAAGAGTCTG TTGAAAAACT GGACTGTCAT TATTCAGGTC ATCATCCTCA GCCAGCATCT 
TTTTGCACAT TTGGGAGCCG GCAGATAGGA AGAGGCTATT ACGTGTTTGA CTCCAGGTGG 
AATCGACTTC GCTGCGCCCT CAACCTCATG GTGGAGAAGC ATCTGAATGC ACAGCTATGG 
AAGAAAATCC CACCAGTGCC CAGTACCACC TCACCCATCT CCACACGTAT TCCTCACCGG 
ACAAACTCTG TGCCGACATC ACAATGTGGA GTCAGCTATC TGGCAGCAGC CACCGTCTCT 
ACATCCCCAG TCCTGCTCTC ATCTACCTGC ATCTCCCCAA ATAGCAAATC GGTACCAGCT 
CATGGAACCA CACTAAATGC ACAGCCTGCT GCTTCAGGGG CGATGGATCC TGTGTGCAGT 
ATGCAATCCA GACAAGTGTC CTCTTCATCC TCATCCCCTT CCACGCCCTC TGGCCTTTCC 
TCGGTTCCTT CCTCCCCCAT GTCCAGGAAA CCTCAGAAAT TGAAATCCAG CAAATCTTTG 
AGGCCCAAGG AGTCTTCTGG TAACAGCACT AACTGTCAAA ATGCCAGTAG CAGTACCAGT 
GGCGGCTCAG GAAAGAAACG CAAAAACAGT TCCCCACTGT TGGTTCACTC TTCCTCCTCC 
TCTTCCTCCT CCTCCTCTTC TTCTCATTCC ATGGAGTCTT TTAGGAAAAA CTGTGTGGCT 
CACTCTGGGC CTCCCTACCC CTCAACGGTA ACATCTTCCC ATAGCATCGG CCTCAACTGT 
GTGACGAATA AAGCAAATGC GGTGAACGTC CGGCATGACC AGTCAGGGAG GGGCCCCCCC 
ACCGGGAGCC CTGCTGAATC CATCAAGAGG ATGAGTGTGA TGGTGAACAG CAGTGATTCT 
ACTCTTTCTC TTGGGCCATT CATTCACCAG TCCAATGAAC TGCCTGTCAA CTCCCACGGC 
AGTTTTTCCC ACTCACACAC TCCTCTAGAC AAACTCATAG GAAAGAAAAG AAAGTGCTCA 
CCCAGCTCGA GCAGCATCAA CAACAGCAGC AGCAAACCCA CAAAGGTTGC CAAAGTGCCA 
GCCGTGAACA ATGTCCACAT GAAACACACA GGCACCATCC CAGGGGCACA AGGACTGATG 
AACAGTTCCC TCCTTCATCA GGATATCTCC TCACCTTGCT TACGAACAGG AATTTCAGCA 
ACATCACCCC AGAGCCCTGA CTTAAAATCC AAAGGCACGT CCCTGACAGC TGAAAATAGC 
ACGGGGAGGA ATAATGCGGA CACTTTTGAG GACAAGTTAC ACCTCCACTC AGCACTCTGG 
ACTCCACGAT GCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001170858.1
CDS	111..2948
Translation	MSERAADDVRGEPRRAAAAAGGAAAAAARQQQQQQQQQQPPPPQPQRQQHPPPPPRRTRPEDGGPGAASTSAAAMATVGERRPLPSPEVMLGQSWNLWVEASKLPGKDGTELDESFKEFGKNREVMGLCREDMPIFGFCPAHDDFYLVVCNDCNQVVKPQAFQSHYERRHSSSSKPPLAVPPTSVFSFFPSLSKSKGGSASGSNRSSSGGVLSASSSSSKLLKSPKEKLQLRGNTRPMHPIQQSRVPHGRIMTPSVKVEKIHPKMDGTLLKSAVGPTCPATVSSLVKPGLNCPSIPKPTLPSPGQILNGKGLPAPPTLEKKPEDNSNNRKFLNKRLSEREFDPDIHCGVIDLDTKKPCTRSLTCKTHSLTQRRAVQGRRKRFDVLLAEHKNKTREKELIRHPDSQQPPQPLRDPHPAPPRTSQEPHQNPHGVIPSESKPFVASKPKPHTPSLPRPPGCPAQQGGSAPIDPPPVHESPHPPLPATEPASRLSSEEGEGDDKEESVEKLDCHYSGHHPQPASFCTFGSRQIGRGYYVFDSRWNRLRCALNLMVEKHLNAQLWKKIPPVPSTTSPISTRIPHRTNSVPTSQCGVSYLAAATVSTSPVLLSSTCISPNSKSVPAHGTTLNAQPAASGAMDPVCSMQSRQVSSSSSSPSTPSGLSSVPSSPMSRKPQKLKSSKSLRPKESSGNSTNCQNASSSTSGGSGKKRKNSSPLLVHSSSSSSSSSSSSHSMESFRKNCVAHSGPPYPSTVTSSHSIGLNCVTNKANAVNVRHDQSGRGPPTGSPAESIKRMSVMVNSSDSTLSLGPFIHQSNELPVNSHGSFSHSHTPLDKLIGKKRKCSPSSSSINNSSSKPTKVAKVPAVNNVHMKHTGTIPGAQGLMNSSLLHQDISSPCLRTGISATSPQSPDLKSKGTSLTAENSTGRNNADTFEDKLHLHSALWTPRCL

Target ORF information:

RefSeq Version	NM_001177387.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177387.1

ORF Insert Sequence:

ATGTCGGAGC GGGCCGCGGA TGACGTCAGG GGGGAGCCGC GCCGCGCGGC GGCGGCGGCG 
GGCGGAGCAG CGGCCGCGGC CGCCCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCG 
CCGCCTCCGC AGCCCCAGCG GCAGCAGCAC CCGCCACCGC CGCCACGGCG CACACGGCCG 
GAGGACGGCG GGCCCGGCGC CGCCTCCACC TCGGCCGCCG CAATGGCGAC GGTCGGGGAG 
CGCAGGCCTC TGCCCAGTCC TGAAGTGATG CTGGGACAGT CGTGGAATCT GTGGGTTGAG 
GCTTCCAAAC TTCCTGGGAA GGACGGGACA GAATTGGACG AAAGTTTCAA GGAGTTTGGG 
AAAAACCGCG AAGTCATGGG GCTCTGTCGG GAAGACATGC CAATATTTGG TTTCTGTCCA 
GCCCATGATG ATTTCTACTT GGTGGTGTGT AACGACTGTA ATCAGGTTGT CAAACCGCAG 
GCATTTCAAT CACATTATGA AAGAAGACAT AGCTCATCCA GCAAGCCGCC TTTGGCCGTT 
CCTCCCACTT CAGTATTTTC CTTCTTCCCT TCTCTGTCCA AAAGCAAAGG AGGCAGTGCA 
AGTGGAAGCA ACCGTTCTTC CAGTGGAGGT GTTCTTAGCG CATCCTCATC AAGTTCCAAG 
TTGTTGAAAT CACCCAAAGA GAAACTGCAG CTCAGGGGGA ACACCAGGCC AATGCATCCC 
ATTCAGCAAA GTAGAGTTCC CCATGGTAGA ATCATGACAC CCTCTGTGAA AGTGGAAAAG 
ATTCATCCGA AAATGGATGG CACACTACTG AAATCTGCGG TGGGGCCAAC CTGTCCTGCT 
ACTGTGAGTT CCTTAGTCAA GCCTGGCCTT AACTGCCCCT CAATACCAAA GCCAACCTTG 
CCTTCACCTG GACAGATTCT GAATGGCAAA GGGCTTCCTG CACCGCCCAC TCTGGAAAAG 
AAACCTGAAG ACAATTCCAA TAATAGGAAA TTTTTAAATA AGAGATTATC AGAAAGAGAG 
TTTGATCCTG ACATCCACTG TGGGGTTATT GATCTCGACA CCAAGAAGCC CTGCACCCGG 
TCTTTGACAT GCAAGACACA TTCCTTAACC CAGCGCAGGG CTGTCCAGGG TAGAAGAAAA 
CGATTTGATG TGTTATTAGC CGAGCACAAA AACAAAACCA GGGAAAAGGA ATTGATTCGC 
CATCCGGACT CTCAGCAACC ACCGCAGCCT CTCAGGGACC CGCATCCCGC CCCTCCTAGA 
ACGTCACAGG AGCCGCACCA AAACCCTCAC GGAGTGATTC CTTCCGAATC AAAGCCTTTT 
GTAGCTAGTA AACCTAAACC TCACACCCCC AGTCTTCCAA GGCCTCCAGG CTGCCCTGCT 
CAGCAAGGTG GGAGTGCCCC CATTGACCCT CCTCCAGTCC ATGAATCTCC ACACCCTCCC 
CTGCCTGCCA CTGAGCCAGC TTCTCGGTTA TCCAGTGAGG AGGGCGAAGG CGATGACAAA 
GAAGAGTCTG TTGAAAAACT GGACTGTCAT TATTCAGGTC ATCATCCTCA GCCAGCATCT 
TTTTGCACAT TTGGGAGCCG GCAGATAGGA AGAGGCTATT ACGTGTTTGA CTCCAGGTGG 
AATCGACTTC GCTGCGCCCT CAACCTCATG GTGGAGAAGC ATCTGAATGC ACAGCTATGG 
AAGAAAATCC CACCAGTGCC CAGTACCACC TCACCCATCT CCACACGTAT TCCTCACCGG 
ACAAACTCTG TGCCGACATC ACAATGTGGA GTCAGCTATC TGGCAGCAGC CACCGTCTCT 
ACATCCCCAG TCCTGCTCTC ATCTACCTGC ATCTCCCCAA ATAGCAAATC GGTACCAGCT 
CATGGAACCA CACTAAATGC ACAGCCTGCT GCTTCAGGGG CGATGGATCC TGTGTGCAGT 
ATGCAATCCA GACAAGTGTC CTCTTCATCC TCATCCCCTT CCACGCCCTC TGGCCTTTCC 
TCGGTTCCTT CCTCCCCCAT GTCCAGGAAA CCTCAGAAAT TGAAATCCAG CAAATCTTTG 
AGGCCCAAGG AGTCTTCTGG TAACAGCACT AACTGTCAAA ATGCCAGTAG CAGTACCAGT 
GGCGGCTCAG GAAAGAAACG CAAAAACAGT TCCCCACTGT TGGTTCACTC TTCCTCCTCC 
TCTTCCTCCT CCTCCTCTTC TTCTCATTCC ATGGAGTCTT TTAGGAAAAA CTGTGTGGCT 
CACTCTGGGC CTCCCTACCC CTCAACGGTA ACATCTTCCC ATAGCATCGG CCTCAACTGT 
GTGACGAATA AAGCAAATGC GGTGAACGTC CGGCATGACC AGTCAGGGAG GGGCCCCCCC 
ACCGGGAGCC CTGCTGAATC CATCAAGAGG ATGAGTGTGA TGGTGAACAG CAGTGATTCT 
ACTCTTTCTC TTGGGCCATT CATTCACCAG TCCAATGAAC TGCCTGTCAA CTCCCACGGC 
AGTTTTTCCC ACTCACACAC TCCTCTAGAC AAACTCATAG GAAAGAAAAG AAAGTGCTCA 
CCCAGCTCGA GCAGCATCAA CAACAGCAGC AGCAAACCCA CAAAGGTTGC CAAAGTGCCA 
GCCGTGAACA ATGTCCACAT GAAACACACA GGCACCATCC CAGGGGCACA AGGACTGATG 
AACAGTTCCC TCCTTCATCA GGATATCTCC TCACCTTGCT TACGAACAGG AATTTCAGCA 
ACATCACCCC AGAGCCCTGA CTTAAAATCC AAAGGCACGT CCCTGACAGC TGAAAATAGC 
ACGGGGAGGA ATAATGCGGA CACTTTTGAG GACAAGTTAC ACCTCCACTC AGCACTCTGG 
ACTCCACGAT GCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATXN7 ( NM_001128149.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121621.2 Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7c, mRNA.

Price & Availability↑

CloneID	OHu20360
Clone ID Related Accession (Same CDS sequence)	NM_001128149.2 , NM_001128149.3
Accession Version	NM_001128149.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	ataxin-7 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP220889.1, AC012557.23, AK304062.1 and BI494079.1. On Apr 7, 2010 this sequence version replaced NM_001128149.1. Transcript Variant: This variant (SCA7c) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant SCA7a. The encoded isoform (c) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304062.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGGTA GCAAGACGCC TCTCCAAAGC AGCCCTTCTG CGCAGGAGCT GAAAGCTCCA 
CTGGAAAGAA GACATAGCTC ATCCAGCAAG CCGCCTTTGG CCGTTCCTCC CACTTCAGTA 
TTTTCCTTCT TCCCTTCTCT GTCCAAAAGC AAAGGAGGCA GTGCAAGTGG AAGCAACCGT 
TCTTCCAGTG GAGGTGTTCT TAGCGCATCC TCATCAAGTT CCAAGTTGTT GAAATCACCC 
AAAGAGAAAC TGCAGCTCAG GGGGAACACC AGGCCAATGC ATCCCATTCA GCAAAGTAGA 
GTTCCCCATG GTAGAATCAT GACACCCTCT GTGAAAGTGG AAAAGATTCA TCCGAAAATG 
GATGGCACAC TACTGAAATC TGCGGTGGGG CCAACCTGTC CTGCTACTGT GAGTTCCTTA 
GTCAAGCCTG GCCTTAACTG CCCCTCAATA CCAAAGCCAA CCTTGCCTTC ACCTGGACAG 
ATTCTGAATG GCAAAGGGCT TCCTGCACCG CCCACTCTGG AAAAGAAACC TGAAGACAAT 
TCCAATAATA GGAAATTTTT AAATAAGAGA TTATCAGAAA GAGAGTTTGA TCCTGACATC 
CACTGTGGGG TTATTGATCT CGACACCAAG AAGCCCTGCA CCCGGTCTTT GACATGCAAG 
ACACATTCCT TAACCCAGCG CAGGGCTGTC CAGGGTAGAA GAAAACGATT TGATGTGTTA 
TTAGCCGAGC ACAAAAACAA AACCAGGGAA AAGGAATTGA TTCGCCATCC GGACTCTCAG 
CAACCACCGC AGCCTCTCAG GGACCCGCAT CCCGCCCCTC CTAGAACGTC ACAGGAGCCG 
CACCAAAACC CTCACGGAGT GATTCCTTCC GAATCAAAGC CTTTTGTAGC TAGTAAACCT 
AAACCTCACA CCCCCAGTCT TCCAAGGCCT CCAGGCTGCC CTGCTCAGCA AGGTGGGAGT 
GCCCCCATTG ACCCTCCTCC AGTCCATGAA TCTCCACACC CTCCCCTGCC TGCCACTGAG 
CCAGCTTCTC GGTTATCCAG TGAGGAGGGC GAAGGCGATG ACAAAGAAGA GTCTGTTGAA 
AAACTGGACT GTCATTATTC AGGTCATCAT CCTCAGCCAG CATCTTTTTG CACATTTGGG 
AGCCGGCAGA TAGGAAGAGG CTATTACGTG TTTGACTCCA GGTGGAATCG ACTTCGCTGC 
GCCCTCAACC TCATGGTGGA GAAGCATCTG AATGCACAGC TATGGAAGAA AATCCCACCA 
GTGCCCAGTA CCACCTCACC CATCTCCACA CGTATTCCTC ACCGGACAAA CTCTGTGCCG 
ACATCACAAT GTGGAGTCAG CTATCTGGCA GCAGCCACCG TCTCTACATC CCCAGTCCTG 
CTCTCATCTA CCTGCATCTC CCCAAATAGC AAATCGGTAC CAGCTCATGG AACCACACTA 
AATGCACAGC CTGCTGCTTC AGGGGCGATG GATCCTGTGT GCAGTATGCA ATCCAGACAA 
GTGTCCTCTT CATCCTCATC CCCTTCCACG CCCTCTGGCC TTTCCTCGGT TCCTTCCTCC 
CCCATGTCCA GGAAACCTCA GAAATTGAAA TCCAGCAAAT CTTTGAGGCC CAAGGAGTCT 
TCTGGTAACA GCACTAACTG TCAAAATGCC AGTAGCAGTA CCAGTGGCGG CTCAGGAAAG 
AAACGCAAAA ACAGTTCCCC ACTGTTGGTT CACTCTTCCT CCTCCTCTTC CTCCTCCTCC 
TCTTCTTCTC ATTCCATGGA GTCTTTTAGG AAAAACTGTG TGGCTCACTC TGGGCCTCCC 
TACCCCTCAA CGGTAACATC TTCCCATAGC ATCGGCCTCA ACTGTGTGAC GAATAAAGCA 
AATGCGGTGA ACGTCCGGCA TGACCAGTCA GGGAGGGGCC CCCCCACCGG GAGCCCTGCT 
GAATCCATCA AGAGGATGAG TGTGATGGTG AACAGCAGTG ATTCTACTCT TTCTCTTGGG 
CCATTCATTC ACCAGTCCAA TGAACTGCCT GTCAACTCCC ACGGCAGTTT TTCCCACTCA 
CACACTCCTC TAGACAAACT CATAGGAAAG AAAAGAAAGT GCTCACCCAG CTCGAGCAGC 
ATCAACAACA GCAGCAGCAA ACCCACAAAG GTTGCCAAAG TGCCAGCCGT GAACAATGTC 
CACATGAAAC ACACAGGCAC CATCCCAGGG GCACAAGGAC TGATGAACAG TTCCCTCCTT 
CATCAGCCAA AGGCACGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121621.2
CDS	169..2412
Translation	MEGSKTPLQSSPSAQELKAPLERRHSSSSKPPLAVPPTSVFSFFPSLSKSKGGSASGSNRSSSGGVLSASSSSSKLLKSPKEKLQLRGNTRPMHPIQQSRVPHGRIMTPSVKVEKIHPKMDGTLLKSAVGPTCPATVSSLVKPGLNCPSIPKPTLPSPGQILNGKGLPAPPTLEKKPEDNSNNRKFLNKRLSEREFDPDIHCGVIDLDTKKPCTRSLTCKTHSLTQRRAVQGRRKRFDVLLAEHKNKTREKELIRHPDSQQPPQPLRDPHPAPPRTSQEPHQNPHGVIPSESKPFVASKPKPHTPSLPRPPGCPAQQGGSAPIDPPPVHESPHPPLPATEPASRLSSEEGEGDDKEESVEKLDCHYSGHHPQPASFCTFGSRQIGRGYYVFDSRWNRLRCALNLMVEKHLNAQLWKKIPPVPSTTSPISTRIPHRTNSVPTSQCGVSYLAAATVSTSPVLLSSTCISPNSKSVPAHGTTLNAQPAASGAMDPVCSMQSRQVSSSSSSPSTPSGLSSVPSSPMSRKPQKLKSSKSLRPKESSGNSTNCQNASSSTSGGSGKKRKNSSPLLVHSSSSSSSSSSSSHSMESFRKNCVAHSGPPYPSTVTSSHSIGLNCVTNKANAVNVRHDQSGRGPPTGSPAESIKRMSVMVNSSDSTLSLGPFIHQSNELPVNSHGSFSHSHTPLDKLIGKKRKCSPSSSSINNSSSKPTKVAKVPAVNNVHMKHTGTIPGAQGLMNSSLLHQPKARP

Target ORF information:

RefSeq Version	NM_001128149.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7c, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128149.2

ORF Insert Sequence:

ATGGAAGGTA GCAAGACGCC TCTCCAAAGC AGCCCTTCTG CGCAGGAGCT GAAAGCTCCA 
CTGGAAAGAA GACATAGCTC ATCCAGCAAG CCGCCTTTGG CCGTTCCTCC CACTTCAGTA 
TTTTCCTTCT TCCCTTCTCT GTCCAAAAGC AAAGGAGGCA GTGCAAGTGG AAGCAACCGT 
TCTTCCAGTG GAGGTGTTCT TAGCGCATCC TCATCAAGTT CCAAGTTGTT GAAATCACCC 
AAAGAGAAAC TGCAGCTCAG GGGGAACACC AGGCCAATGC ATCCCATTCA GCAAAGTAGA 
GTTCCCCATG GTAGAATCAT GACACCCTCT GTGAAAGTGG AAAAGATTCA TCCGAAAATG 
GATGGCACAC TACTGAAATC TGCGGTGGGG CCAACCTGTC CTGCTACTGT GAGTTCCTTA 
GTCAAGCCTG GCCTTAACTG CCCCTCAATA CCAAAGCCAA CCTTGCCTTC ACCTGGACAG 
ATTCTGAATG GCAAAGGGCT TCCTGCACCG CCCACTCTGG AAAAGAAACC TGAAGACAAT 
TCCAATAATA GGAAATTTTT AAATAAGAGA TTATCAGAAA GAGAGTTTGA TCCTGACATC 
CACTGTGGGG TTATTGATCT CGACACCAAG AAGCCCTGCA CCCGGTCTTT GACATGCAAG 
ACACATTCCT TAACCCAGCG CAGGGCTGTC CAGGGTAGAA GAAAACGATT TGATGTGTTA 
TTAGCCGAGC ACAAAAACAA AACCAGGGAA AAGGAATTGA TTCGCCATCC GGACTCTCAG 
CAACCACCGC AGCCTCTCAG GGACCCGCAT CCCGCCCCTC CTAGAACGTC ACAGGAGCCG 
CACCAAAACC CTCACGGAGT GATTCCTTCC GAATCAAAGC CTTTTGTAGC TAGTAAACCT 
AAACCTCACA CCCCCAGTCT TCCAAGGCCT CCAGGCTGCC CTGCTCAGCA AGGTGGGAGT 
GCCCCCATTG ACCCTCCTCC AGTCCATGAA TCTCCACACC CTCCCCTGCC TGCCACTGAG 
CCAGCTTCTC GGTTATCCAG TGAGGAGGGC GAAGGCGATG ACAAAGAAGA GTCTGTTGAA 
AAACTGGACT GTCATTATTC AGGTCATCAT CCTCAGCCAG CATCTTTTTG CACATTTGGG 
AGCCGGCAGA TAGGAAGAGG CTATTACGTG TTTGACTCCA GGTGGAATCG ACTTCGCTGC 
GCCCTCAACC TCATGGTGGA GAAGCATCTG AATGCACAGC TATGGAAGAA AATCCCACCA 
GTGCCCAGTA CCACCTCACC CATCTCCACA CGTATTCCTC ACCGGACAAA CTCTGTGCCG 
ACATCACAAT GTGGAGTCAG CTATCTGGCA GCAGCCACCG TCTCTACATC CCCAGTCCTG 
CTCTCATCTA CCTGCATCTC CCCAAATAGC AAATCGGTAC CAGCTCATGG AACCACACTA 
AATGCACAGC CTGCTGCTTC AGGGGCGATG GATCCTGTGT GCAGTATGCA ATCCAGACAA 
GTGTCCTCTT CATCCTCATC CCCTTCCACG CCCTCTGGCC TTTCCTCGGT TCCTTCCTCC 
CCCATGTCCA GGAAACCTCA GAAATTGAAA TCCAGCAAAT CTTTGAGGCC CAAGGAGTCT 
TCTGGTAACA GCACTAACTG TCAAAATGCC AGTAGCAGTA CCAGTGGCGG CTCAGGAAAG 
AAACGCAAAA ACAGTTCCCC ACTGTTGGTT CACTCTTCCT CCTCCTCTTC CTCCTCCTCC 
TCTTCTTCTC ATTCCATGGA GTCTTTTAGG AAAAACTGTG TGGCTCACTC TGGGCCTCCC 
TACCCCTCAA CGGTAACATC TTCCCATAGC ATCGGCCTCA ACTGTGTGAC GAATAAAGCA 
AATGCGGTGA ACGTCCGGCA TGACCAGTCA GGGAGGGGCC CCCCCACCGG GAGCCCTGCT 
GAATCCATCA AGAGGATGAG TGTGATGGTG AACAGCAGTG ATTCTACTCT TTCTCTTGGG 
CCATTCATTC ACCAGTCCAA TGAACTGCCT GTCAACTCCC ACGGCAGTTT TTCCCACTCA 
CACACTCCTC TAGACAAACT CATAGGAAAG AAAAGAAAGT GCTCACCCAG CTCGAGCAGC 
ATCAACAACA GCAGCAGCAA ACCCACAAAG GTTGCCAAAG TGCCAGCCGT GAACAATGTC 
CACATGAAAC ACACAGGCAC CATCCCAGGG GCACAAGGAC TGATGAACAG TTCCCTCCTT 
CATCAGCCAA AGGCACGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATXN7 ( NM_001128149.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121621.2 Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7c, mRNA.

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CloneID	OHu20360
Clone ID Related Accession (Same CDS sequence)	NM_001128149.2 , NM_001128149.3
Accession Version	NM_001128149.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	ataxin-7 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012557.23, AK304062.1 and BI494079.1. On Jun 2, 2019 this sequence version replaced NM_001128149.2. Transcript Variant: This variant (SCA7c) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant SCA7a. The encoded isoform (c) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304062.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGGTA GCAAGACGCC TCTCCAAAGC AGCCCTTCTG CGCAGGAGCT GAAAGCTCCA 
CTGGAAAGAA GACATAGCTC ATCCAGCAAG CCGCCTTTGG CCGTTCCTCC CACTTCAGTA 
TTTTCCTTCT TCCCTTCTCT GTCCAAAAGC AAAGGAGGCA GTGCAAGTGG AAGCAACCGT 
TCTTCCAGTG GAGGTGTTCT TAGCGCATCC TCATCAAGTT CCAAGTTGTT GAAATCACCC 
AAAGAGAAAC TGCAGCTCAG GGGGAACACC AGGCCAATGC ATCCCATTCA GCAAAGTAGA 
GTTCCCCATG GTAGAATCAT GACACCCTCT GTGAAAGTGG AAAAGATTCA TCCGAAAATG 
GATGGCACAC TACTGAAATC TGCGGTGGGG CCAACCTGTC CTGCTACTGT GAGTTCCTTA 
GTCAAGCCTG GCCTTAACTG CCCCTCAATA CCAAAGCCAA CCTTGCCTTC ACCTGGACAG 
ATTCTGAATG GCAAAGGGCT TCCTGCACCG CCCACTCTGG AAAAGAAACC TGAAGACAAT 
TCCAATAATA GGAAATTTTT AAATAAGAGA TTATCAGAAA GAGAGTTTGA TCCTGACATC 
CACTGTGGGG TTATTGATCT CGACACCAAG AAGCCCTGCA CCCGGTCTTT GACATGCAAG 
ACACATTCCT TAACCCAGCG CAGGGCTGTC CAGGGTAGAA GAAAACGATT TGATGTGTTA 
TTAGCCGAGC ACAAAAACAA AACCAGGGAA AAGGAATTGA TTCGCCATCC GGACTCTCAG 
CAACCACCGC AGCCTCTCAG GGACCCGCAT CCCGCCCCTC CTAGAACGTC ACAGGAGCCG 
CACCAAAACC CTCACGGAGT GATTCCTTCC GAATCAAAGC CTTTTGTAGC TAGTAAACCT 
AAACCTCACA CCCCCAGTCT TCCAAGGCCT CCAGGCTGCC CTGCTCAGCA AGGTGGGAGT 
GCCCCCATTG ACCCTCCTCC AGTCCATGAA TCTCCACACC CTCCCCTGCC TGCCACTGAG 
CCAGCTTCTC GGTTATCCAG TGAGGAGGGC GAAGGCGATG ACAAAGAAGA GTCTGTTGAA 
AAACTGGACT GTCATTATTC AGGTCATCAT CCTCAGCCAG CATCTTTTTG CACATTTGGG 
AGCCGGCAGA TAGGAAGAGG CTATTACGTG TTTGACTCCA GGTGGAATCG ACTTCGCTGC 
GCCCTCAACC TCATGGTGGA GAAGCATCTG AATGCACAGC TATGGAAGAA AATCCCACCA 
GTGCCCAGTA CCACCTCACC CATCTCCACA CGTATTCCTC ACCGGACAAA CTCTGTGCCG 
ACATCACAAT GTGGAGTCAG CTATCTGGCA GCAGCCACCG TCTCTACATC CCCAGTCCTG 
CTCTCATCTA CCTGCATCTC CCCAAATAGC AAATCGGTAC CAGCTCATGG AACCACACTA 
AATGCACAGC CTGCTGCTTC AGGGGCGATG GATCCTGTGT GCAGTATGCA ATCCAGACAA 
GTGTCCTCTT CATCCTCATC CCCTTCCACG CCCTCTGGCC TTTCCTCGGT TCCTTCCTCC 
CCCATGTCCA GGAAACCTCA GAAATTGAAA TCCAGCAAAT CTTTGAGGCC CAAGGAGTCT 
TCTGGTAACA GCACTAACTG TCAAAATGCC AGTAGCAGTA CCAGTGGCGG CTCAGGAAAG 
AAACGCAAAA ACAGTTCCCC ACTGTTGGTT CACTCTTCCT CCTCCTCTTC CTCCTCCTCC 
TCTTCTTCTC ATTCCATGGA GTCTTTTAGG AAAAACTGTG TGGCTCACTC TGGGCCTCCC 
TACCCCTCAA CGGTAACATC TTCCCATAGC ATCGGCCTCA ACTGTGTGAC GAATAAAGCA 
AATGCGGTGA ACGTCCGGCA TGACCAGTCA GGGAGGGGCC CCCCCACCGG GAGCCCTGCT 
GAATCCATCA AGAGGATGAG TGTGATGGTG AACAGCAGTG ATTCTACTCT TTCTCTTGGG 
CCATTCATTC ACCAGTCCAA TGAACTGCCT GTCAACTCCC ACGGCAGTTT TTCCCACTCA 
CACACTCCTC TAGACAAACT CATAGGAAAG AAAAGAAAGT GCTCACCCAG CTCGAGCAGC 
ATCAACAACA GCAGCAGCAA ACCCACAAAG GTTGCCAAAG TGCCAGCCGT GAACAATGTC 
CACATGAAAC ACACAGGCAC CATCCCAGGG GCACAAGGAC TGATGAACAG TTCCCTCCTT 
CATCAGCCAA AGGCACGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121621.2
CDS	119..2362
Translation	MEGSKTPLQSSPSAQELKAPLERRHSSSSKPPLAVPPTSVFSFFPSLSKSKGGSASGSNRSSSGGVLSASSSSSKLLKSPKEKLQLRGNTRPMHPIQQSRVPHGRIMTPSVKVEKIHPKMDGTLLKSAVGPTCPATVSSLVKPGLNCPSIPKPTLPSPGQILNGKGLPAPPTLEKKPEDNSNNRKFLNKRLSEREFDPDIHCGVIDLDTKKPCTRSLTCKTHSLTQRRAVQGRRKRFDVLLAEHKNKTREKELIRHPDSQQPPQPLRDPHPAPPRTSQEPHQNPHGVIPSESKPFVASKPKPHTPSLPRPPGCPAQQGGSAPIDPPPVHESPHPPLPATEPASRLSSEEGEGDDKEESVEKLDCHYSGHHPQPASFCTFGSRQIGRGYYVFDSRWNRLRCALNLMVEKHLNAQLWKKIPPVPSTTSPISTRIPHRTNSVPTSQCGVSYLAAATVSTSPVLLSSTCISPNSKSVPAHGTTLNAQPAASGAMDPVCSMQSRQVSSSSSSPSTPSGLSSVPSSPMSRKPQKLKSSKSLRPKESSGNSTNCQNASSSTSGGSGKKRKNSSPLLVHSSSSSSSSSSSSHSMESFRKNCVAHSGPPYPSTVTSSHSIGLNCVTNKANAVNVRHDQSGRGPPTGSPAESIKRMSVMVNSSDSTLSLGPFIHQSNELPVNSHGSFSHSHTPLDKLIGKKRKCSPSSSSINNSSSKPTKVAKVPAVNNVHMKHTGTIPGAQGLMNSSLLHQPKARP

Target ORF information:

RefSeq Version	NM_001128149.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7c, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128149.3

ORF Insert Sequence:

ATGGAAGGTA GCAAGACGCC TCTCCAAAGC AGCCCTTCTG CGCAGGAGCT GAAAGCTCCA 
CTGGAAAGAA GACATAGCTC ATCCAGCAAG CCGCCTTTGG CCGTTCCTCC CACTTCAGTA 
TTTTCCTTCT TCCCTTCTCT GTCCAAAAGC AAAGGAGGCA GTGCAAGTGG AAGCAACCGT 
TCTTCCAGTG GAGGTGTTCT TAGCGCATCC TCATCAAGTT CCAAGTTGTT GAAATCACCC 
AAAGAGAAAC TGCAGCTCAG GGGGAACACC AGGCCAATGC ATCCCATTCA GCAAAGTAGA 
GTTCCCCATG GTAGAATCAT GACACCCTCT GTGAAAGTGG AAAAGATTCA TCCGAAAATG 
GATGGCACAC TACTGAAATC TGCGGTGGGG CCAACCTGTC CTGCTACTGT GAGTTCCTTA 
GTCAAGCCTG GCCTTAACTG CCCCTCAATA CCAAAGCCAA CCTTGCCTTC ACCTGGACAG 
ATTCTGAATG GCAAAGGGCT TCCTGCACCG CCCACTCTGG AAAAGAAACC TGAAGACAAT 
TCCAATAATA GGAAATTTTT AAATAAGAGA TTATCAGAAA GAGAGTTTGA TCCTGACATC 
CACTGTGGGG TTATTGATCT CGACACCAAG AAGCCCTGCA CCCGGTCTTT GACATGCAAG 
ACACATTCCT TAACCCAGCG CAGGGCTGTC CAGGGTAGAA GAAAACGATT TGATGTGTTA 
TTAGCCGAGC ACAAAAACAA AACCAGGGAA AAGGAATTGA TTCGCCATCC GGACTCTCAG 
CAACCACCGC AGCCTCTCAG GGACCCGCAT CCCGCCCCTC CTAGAACGTC ACAGGAGCCG 
CACCAAAACC CTCACGGAGT GATTCCTTCC GAATCAAAGC CTTTTGTAGC TAGTAAACCT 
AAACCTCACA CCCCCAGTCT TCCAAGGCCT CCAGGCTGCC CTGCTCAGCA AGGTGGGAGT 
GCCCCCATTG ACCCTCCTCC AGTCCATGAA TCTCCACACC CTCCCCTGCC TGCCACTGAG 
CCAGCTTCTC GGTTATCCAG TGAGGAGGGC GAAGGCGATG ACAAAGAAGA GTCTGTTGAA 
AAACTGGACT GTCATTATTC AGGTCATCAT CCTCAGCCAG CATCTTTTTG CACATTTGGG 
AGCCGGCAGA TAGGAAGAGG CTATTACGTG TTTGACTCCA GGTGGAATCG ACTTCGCTGC 
GCCCTCAACC TCATGGTGGA GAAGCATCTG AATGCACAGC TATGGAAGAA AATCCCACCA 
GTGCCCAGTA CCACCTCACC CATCTCCACA CGTATTCCTC ACCGGACAAA CTCTGTGCCG 
ACATCACAAT GTGGAGTCAG CTATCTGGCA GCAGCCACCG TCTCTACATC CCCAGTCCTG 
CTCTCATCTA CCTGCATCTC CCCAAATAGC AAATCGGTAC CAGCTCATGG AACCACACTA 
AATGCACAGC CTGCTGCTTC AGGGGCGATG GATCCTGTGT GCAGTATGCA ATCCAGACAA 
GTGTCCTCTT CATCCTCATC CCCTTCCACG CCCTCTGGCC TTTCCTCGGT TCCTTCCTCC 
CCCATGTCCA GGAAACCTCA GAAATTGAAA TCCAGCAAAT CTTTGAGGCC CAAGGAGTCT 
TCTGGTAACA GCACTAACTG TCAAAATGCC AGTAGCAGTA CCAGTGGCGG CTCAGGAAAG 
AAACGCAAAA ACAGTTCCCC ACTGTTGGTT CACTCTTCCT CCTCCTCTTC CTCCTCCTCC 
TCTTCTTCTC ATTCCATGGA GTCTTTTAGG AAAAACTGTG TGGCTCACTC TGGGCCTCCC 
TACCCCTCAA CGGTAACATC TTCCCATAGC ATCGGCCTCA ACTGTGTGAC GAATAAAGCA 
AATGCGGTGA ACGTCCGGCA TGACCAGTCA GGGAGGGGCC CCCCCACCGG GAGCCCTGCT 
GAATCCATCA AGAGGATGAG TGTGATGGTG AACAGCAGTG ATTCTACTCT TTCTCTTGGG 
CCATTCATTC ACCAGTCCAA TGAACTGCCT GTCAACTCCC ACGGCAGTTT TTCCCACTCA 
CACACTCCTC TAGACAAACT CATAGGAAAG AAAAGAAAGT GCTCACCCAG CTCGAGCAGC 
ATCAACAACA GCAGCAGCAA ACCCACAAAG GTTGCCAAAG TGCCAGCCGT GAACAATGTC 
CACATGAAAC ACACAGGCAC CATCCCAGGG GCACAAGGAC TGATGAACAG TTCCCTCCTT 
CATCAGCCAA AGGCACGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATXN7 ( NM_000333.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000324.1 Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7a, mRNA.

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CloneID	OHu26052
Clone ID Related Accession (Same CDS sequence)	NM_000333.3
Accession Version	NM_000333.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	ataxin-7 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF032104.1, AJ000517.1, AC012557.23 and BI494079.1. This sequence is a reference standard in the RefSeqGene project. On Jun 8, 2008 this sequence version replaced NM_000333.2. Transcript Variant: This variant (SCA7a) represents the predominant transcript and encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ000517.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGC GGGCCGCGGA TGACGTCAGG GGGGAGCCGC GCCGCGCGGC GGCGGCGGCG 
GGCGGAGCAG CGGCCGCGGC CGCCCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCG 
CCGCCTCCGC AGCCCCAGCG GCAGCAGCAC CCGCCACCGC CGCCACGGCG CACACGGCCG 
GAGGACGGCG GGCCCGGCGC CGCCTCCACC TCGGCCGCCG CAATGGCGAC GGTCGGGGAG 
CGCAGGCCTC TGCCCAGTCC TGAAGTGATG CTGGGACAGT CGTGGAATCT GTGGGTTGAG 
GCTTCCAAAC TTCCTGGGAA GGACGGGACA GAATTGGACG AAAGTTTCAA GGAGTTTGGG 
AAAAACCGCG AAGTCATGGG GCTCTGTCGG GAAGACATGC CAATATTTGG TTTCTGTCCA 
GCCCATGATG ATTTCTACTT GGTGGTGTGT AACGACTGTA ATCAGGTTGT CAAACCGCAG 
GCATTTCAAT CACATTATGA AAGAAGACAT AGCTCATCCA GCAAGCCGCC TTTGGCCGTT 
CCTCCCACTT CAGTATTTTC CTTCTTCCCT TCTCTGTCCA AAAGCAAAGG AGGCAGTGCA 
AGTGGAAGCA ACCGTTCTTC CAGTGGAGGT GTTCTTAGCG CATCCTCATC AAGTTCCAAG 
TTGTTGAAAT CACCCAAAGA GAAACTGCAG CTCAGGGGGA ACACCAGGCC AATGCATCCC 
ATTCAGCAAA GTAGAGTTCC CCATGGTAGA ATCATGACAC CCTCTGTGAA AGTGGAAAAG 
ATTCATCCGA AAATGGATGG CACACTACTG AAATCTGCGG TGGGGCCAAC CTGTCCTGCT 
ACTGTGAGTT CCTTAGTCAA GCCTGGCCTT AACTGCCCCT CAATACCAAA GCCAACCTTG 
CCTTCACCTG GACAGATTCT GAATGGCAAA GGGCTTCCTG CACCGCCCAC TCTGGAAAAG 
AAACCTGAAG ACAATTCCAA TAATAGGAAA TTTTTAAATA AGAGATTATC AGAAAGAGAG 
TTTGATCCTG ACATCCACTG TGGGGTTATT GATCTCGACA CCAAGAAGCC CTGCACCCGG 
TCTTTGACAT GCAAGACACA TTCCTTAACC CAGCGCAGGG CTGTCCAGGG TAGAAGAAAA 
CGATTTGATG TGTTATTAGC CGAGCACAAA AACAAAACCA GGGAAAAGGA ATTGATTCGC 
CATCCGGACT CTCAGCAACC ACCGCAGCCT CTCAGGGACC CGCATCCCGC CCCTCCTAGA 
ACGTCACAGG AGCCGCACCA AAACCCTCAC GGAGTGATTC CTTCCGAATC AAAGCCTTTT 
GTAGCTAGTA AACCTAAACC TCACACCCCC AGTCTTCCAA GGCCTCCAGG CTGCCCTGCT 
CAGCAAGGTG GGAGTGCCCC CATTGACCCT CCTCCAGTCC ATGAATCTCC ACACCCTCCC 
CTGCCTGCCA CTGAGCCAGC TTCTCGGTTA TCCAGTGAGG AGGGCGAAGG CGATGACAAA 
GAAGAGTCTG TTGAAAAACT GGACTGTCAT TATTCAGGTC ATCATCCTCA GCCAGCATCT 
TTTTGCACAT TTGGGAGCCG GCAGATAGGA AGAGGCTATT ACGTGTTTGA CTCCAGGTGG 
AATCGACTTC GCTGCGCCCT CAACCTCATG GTGGAGAAGC ATCTGAATGC ACAGCTATGG 
AAGAAAATCC CACCAGTGCC CAGTACCACC TCACCCATCT CCACACGTAT TCCTCACCGG 
ACAAACTCTG TGCCGACATC ACAATGTGGA GTCAGCTATC TGGCAGCAGC CACCGTCTCT 
ACATCCCCAG TCCTGCTCTC ATCTACCTGC ATCTCCCCAA ATAGCAAATC GGTACCAGCT 
CATGGAACCA CACTAAATGC ACAGCCTGCT GCTTCAGGGG CGATGGATCC TGTGTGCAGT 
ATGCAATCCA GACAAGTGTC CTCTTCATCC TCATCCCCTT CCACGCCCTC TGGCCTTTCC 
TCGGTTCCTT CCTCCCCCAT GTCCAGGAAA CCTCAGAAAT TGAAATCCAG CAAATCTTTG 
AGGCCCAAGG AGTCTTCTGG TAACAGCACT AACTGTCAAA ATGCCAGTAG CAGTACCAGT 
GGCGGCTCAG GAAAGAAACG CAAAAACAGT TCCCCACTGT TGGTTCACTC TTCCTCCTCC 
TCTTCCTCCT CCTCCTCTTC TTCTCATTCC ATGGAGTCTT TTAGGAAAAA CTGTGTGGCT 
CACTCTGGGC CTCCCTACCC CTCAACGGTA ACATCTTCCC ATAGCATCGG CCTCAACTGT 
GTGACGAATA AAGCAAATGC GGTGAACGTC CGGCATGACC AGTCAGGGAG GGGCCCCCCC 
ACCGGGAGCC CTGCTGAATC CATCAAGAGG ATGAGTGTGA TGGTGAACAG CAGTGATTCT 
ACTCTTTCTC TTGGGCCATT CATTCACCAG TCCAATGAAC TGCCTGTCAA CTCCCACGGC 
AGTTTTTCCC ACTCACACAC TCCTCTAGAC AAACTCATAG GAAAGAAAAG AAAGTGCTCA 
CCCAGCTCGA GCAGCATCAA CAACAGCAGC AGCAAACCCA CAAAGGTTGC CAAAGTGCCA 
GCCGTGAACA ATGTCCACAT GAAACACACA GGCACCATCC CAGGGGCACA AGGACTGATG 
AACAGTTCCC TCCTTCATCA GCCAAAGGCA CGTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000324.1
CDS	554..3232
Translation	MSERAADDVRGEPRRAAAAAGGAAAAAARQQQQQQQQQQPPPPQPQRQQHPPPPPRRTRPEDGGPGAASTSAAAMATVGERRPLPSPEVMLGQSWNLWVEASKLPGKDGTELDESFKEFGKNREVMGLCREDMPIFGFCPAHDDFYLVVCNDCNQVVKPQAFQSHYERRHSSSSKPPLAVPPTSVFSFFPSLSKSKGGSASGSNRSSSGGVLSASSSSSKLLKSPKEKLQLRGNTRPMHPIQQSRVPHGRIMTPSVKVEKIHPKMDGTLLKSAVGPTCPATVSSLVKPGLNCPSIPKPTLPSPGQILNGKGLPAPPTLEKKPEDNSNNRKFLNKRLSEREFDPDIHCGVIDLDTKKPCTRSLTCKTHSLTQRRAVQGRRKRFDVLLAEHKNKTREKELIRHPDSQQPPQPLRDPHPAPPRTSQEPHQNPHGVIPSESKPFVASKPKPHTPSLPRPPGCPAQQGGSAPIDPPPVHESPHPPLPATEPASRLSSEEGEGDDKEESVEKLDCHYSGHHPQPASFCTFGSRQIGRGYYVFDSRWNRLRCALNLMVEKHLNAQLWKKIPPVPSTTSPISTRIPHRTNSVPTSQCGVSYLAAATVSTSPVLLSSTCISPNSKSVPAHGTTLNAQPAASGAMDPVCSMQSRQVSSSSSSPSTPSGLSSVPSSPMSRKPQKLKSSKSLRPKESSGNSTNCQNASSSTSGGSGKKRKNSSPLLVHSSSSSSSSSSSSHSMESFRKNCVAHSGPPYPSTVTSSHSIGLNCVTNKANAVNVRHDQSGRGPPTGSPAESIKRMSVMVNSSDSTLSLGPFIHQSNELPVNSHGSFSHSHTPLDKLIGKKRKCSPSSSSINNSSSKPTKVAKVPAVNNVHMKHTGTIPGAQGLMNSSLLHQPKARP

Target ORF information:

RefSeq Version	NM_000333.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ataxin 7 (ATXN7), transcript variant SCA7a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000333.3

ORF Insert Sequence:

ATGTCGGAGC GGGCCGCGGA TGACGTCAGG GGGGAGCCGC GCCGCGCGGC GGCGGCGGCG 
GGCGGAGCAG CGGCCGCGGC CGCCCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCG 
CCGCCTCCGC AGCCCCAGCG GCAGCAGCAC CCGCCACCGC CGCCACGGCG CACACGGCCG 
GAGGACGGCG GGCCCGGCGC CGCCTCCACC TCGGCCGCCG CAATGGCGAC GGTCGGGGAG 
CGCAGGCCTC TGCCCAGTCC TGAAGTGATG CTGGGACAGT CGTGGAATCT GTGGGTTGAG 
GCTTCCAAAC TTCCTGGGAA GGACGGGACA GAATTGGACG AAAGTTTCAA GGAGTTTGGG 
AAAAACCGCG AAGTCATGGG GCTCTGTCGG GAAGACATGC CAATATTTGG TTTCTGTCCA 
GCCCATGATG ATTTCTACTT GGTGGTGTGT AACGACTGTA ATCAGGTTGT CAAACCGCAG 
GCATTTCAAT CACATTATGA AAGAAGACAT AGCTCATCCA GCAAGCCGCC TTTGGCCGTT 
CCTCCCACTT CAGTATTTTC CTTCTTCCCT TCTCTGTCCA AAAGCAAAGG AGGCAGTGCA 
AGTGGAAGCA ACCGTTCTTC CAGTGGAGGT GTTCTTAGCG CATCCTCATC AAGTTCCAAG 
TTGTTGAAAT CACCCAAAGA GAAACTGCAG CTCAGGGGGA ACACCAGGCC AATGCATCCC 
ATTCAGCAAA GTAGAGTTCC CCATGGTAGA ATCATGACAC CCTCTGTGAA AGTGGAAAAG 
ATTCATCCGA AAATGGATGG CACACTACTG AAATCTGCGG TGGGGCCAAC CTGTCCTGCT 
ACTGTGAGTT CCTTAGTCAA GCCTGGCCTT AACTGCCCCT CAATACCAAA GCCAACCTTG 
CCTTCACCTG GACAGATTCT GAATGGCAAA GGGCTTCCTG CACCGCCCAC TCTGGAAAAG 
AAACCTGAAG ACAATTCCAA TAATAGGAAA TTTTTAAATA AGAGATTATC AGAAAGAGAG 
TTTGATCCTG ACATCCACTG TGGGGTTATT GATCTCGACA CCAAGAAGCC CTGCACCCGG 
TCTTTGACAT GCAAGACACA TTCCTTAACC CAGCGCAGGG CTGTCCAGGG TAGAAGAAAA 
CGATTTGATG TGTTATTAGC CGAGCACAAA AACAAAACCA GGGAAAAGGA ATTGATTCGC 
CATCCGGACT CTCAGCAACC ACCGCAGCCT CTCAGGGACC CGCATCCCGC CCCTCCTAGA 
ACGTCACAGG AGCCGCACCA AAACCCTCAC GGAGTGATTC CTTCCGAATC AAAGCCTTTT 
GTAGCTAGTA AACCTAAACC TCACACCCCC AGTCTTCCAA GGCCTCCAGG CTGCCCTGCT 
CAGCAAGGTG GGAGTGCCCC CATTGACCCT CCTCCAGTCC ATGAATCTCC ACACCCTCCC 
CTGCCTGCCA CTGAGCCAGC TTCTCGGTTA TCCAGTGAGG AGGGCGAAGG CGATGACAAA 
GAAGAGTCTG TTGAAAAACT GGACTGTCAT TATTCAGGTC ATCATCCTCA GCCAGCATCT 
TTTTGCACAT TTGGGAGCCG GCAGATAGGA AGAGGCTATT ACGTGTTTGA CTCCAGGTGG 
AATCGACTTC GCTGCGCCCT CAACCTCATG GTGGAGAAGC ATCTGAATGC ACAGCTATGG 
AAGAAAATCC CACCAGTGCC CAGTACCACC TCACCCATCT CCACACGTAT TCCTCACCGG 
ACAAACTCTG TGCCGACATC ACAATGTGGA GTCAGCTATC TGGCAGCAGC CACCGTCTCT 
ACATCCCCAG TCCTGCTCTC ATCTACCTGC ATCTCCCCAA ATAGCAAATC GGTACCAGCT 
CATGGAACCA CACTAAATGC ACAGCCTGCT GCTTCAGGGG CGATGGATCC TGTGTGCAGT 
ATGCAATCCA GACAAGTGTC CTCTTCATCC TCATCCCCTT CCACGCCCTC TGGCCTTTCC 
TCGGTTCCTT CCTCCCCCAT GTCCAGGAAA CCTCAGAAAT TGAAATCCAG CAAATCTTTG 
AGGCCCAAGG AGTCTTCTGG TAACAGCACT AACTGTCAAA ATGCCAGTAG CAGTACCAGT 
GGCGGCTCAG GAAAGAAACG CAAAAACAGT TCCCCACTGT TGGTTCACTC TTCCTCCTCC 
TCTTCCTCCT CCTCCTCTTC TTCTCATTCC ATGGAGTCTT TTAGGAAAAA CTGTGTGGCT 
CACTCTGGGC CTCCCTACCC CTCAACGGTA ACATCTTCCC ATAGCATCGG CCTCAACTGT 
GTGACGAATA AAGCAAATGC GGTGAACGTC CGGCATGACC AGTCAGGGAG GGGCCCCCCC 
ACCGGGAGCC CTGCTGAATC CATCAAGAGG ATGAGTGTGA TGGTGAACAG CAGTGATTCT 
ACTCTTTCTC TTGGGCCATT CATTCACCAG TCCAATGAAC TGCCTGTCAA CTCCCACGGC 
AGTTTTTCCC ACTCACACAC TCCTCTAGAC AAACTCATAG GAAAGAAAAG AAAGTGCTCA 
CCCAGCTCGA GCAGCATCAA CAACAGCAGC AGCAAACCCA CAAAGGTTGC CAAAGTGCCA 
GCCGTGAACA ATGTCCACAT GAAACACACA GGCACCATCC CAGGGGCACA AGGACTGATG 
AACAGTTCCC TCCTTCATCA GCCAAAGGCA CGTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Complete Association of an intronic SNP rs6798742 with Origin of Spinocerebellar Ataxia Type 7-CAG Expansion Loci in the Indian and Mexican Population.
Annals of human genetics81(5)197-204(2017 Sep)
Faruq M,Maga?a JJ,Suroliya V,Narang A,Murillo-Melo NM,Hern?ndez-Hern?ndez O,Srivastava AK,Mukerji M

Spinocerebellar Ataxia.
Ophthalmology124(7)1071(2017 Jul)
Wentz S,Jusufbegovic D

Large normal-range TBP and ATXN7 CAG repeat lengths are associated with increased lifetime risk of depression.
Translational psychiatry7(6)e1143(2017 Jun)
Gardiner SL,van Belzen MJ,Boogaard MW,van Roon-Mom WMC,Rozing MP,van Hemert AM,Smit JH,Beekman ATF,van Grootheest G,Schoevers RA,Oude Voshaar RC,Comijs HC,Penninx BWJH,van der Mast RC,Roos RAC,Aziz NA

ATXN7 Gene Variants and Expression Predict Post-Operative Clinical Outcomes in Hepatitis B Virus-Related Hepatocellular Carcinoma.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology39(6)2427-2438(2016)
Han C,Yu L,Liu X,Yu T,Qin W,Liao X,Liu Z,Lu S,Chen Z,Su H,Zhu G,Qin X,Gui Y,Li J,Xiao K,Chen X,Ye X,Peng M,Dong J,Peng T

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ATXN7 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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