RYK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RYK
Entrez Gene ID	6259
Full Name	receptor-like tyrosine kinase
Synonyms	D3S3195,JTK5,JTK5A,RYK1
General protein information	Preferred Names receptor-like tyrosine kinase Names tyrosine-protein kinase RYK JTK5A protein tyrosine kinase hydroxyaryl-protein kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q22.2
Summary	The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012].
Disorder MIM:	600524

mRNA and Protein(s)

mRNA	Protein	Name
XM_017007016.1	XP_016862505.1	tyrosine-protein kinase RYK isoform X1
NM_002958.3	NP_002949.2	tyrosine-protein kinase RYK isoform 2 precursor
NM_001005861.2	NP_001005861.1	tyrosine-protein kinase RYK isoform 1 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ryk	NP_038677.3
Bos taurus (cattle)	RYK	XP_002685146.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006405	XP_316438.5
Pan troglodytes (chimpanzee)	RYK	XP_003310084.1
Homo sapiens (human)	RYK	NP_001005861.1
Gallus gallus (chicken)	RYK	XP_422674.4
Caenorhabditis elegans (roundworm)	lin-18	NP_508684.4
Xenopus tropicalis (tropical clawed frog)	ryk	NP_001017272.1
Canis lupus familiaris (dog)	RYK	XP_005634509.1
Rattus norvegicus (Norway rat)	Ryk	NP_536327.1
Danio rerio (zebrafish)	ryk	NP_001077295.1
Drosophila melanogaster (fruit fly)	Drl-2	NP_610835.2

Gene Ontology
Bibliography

Related articles in PubMed

RYK promotes the stemness of glioblastoma cells via the WNT/ ?-catenin pathway.
Adamo A, Fiore D, De Martino F, Roscigno G, Affinito A, Donnarumma E, Puoti I, Ricci Vitiani L, Pallini R, Quintavalle C, Condorelli G
Oncotarget8(8)13476-13487(2017 Feb)

Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells.
Skaria T, Bachli E, Schoedon G
Cell adhesion & migration11(1)24-38(2017 Jan)

Wnt5a, Ryk and Ror2 expression in glioblastoma subgroups.
Kim Y, Hong M, Do IG, Ha SY, Lee D, Suh YL
Pathology, research and practice211(12)963-72(2015 Dec)

Human epicardial cell-conditioned medium contains HGF/IgG complexes that phosphorylate RYK and protect against vascular injury.
Rao KS, Aronshtam A, McElory-Yaggy KL, Bakondi B, VanBuren P, Sobel BE, Spees JL
Cardiovascular research107(2)277-86(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our findings demonstrate that Ryk promotes stem cell-like and tumorigenic features to glioma cells its essential for the maintenance of glioblastoma stem cells
Title: RYK promotes the stemness of glioblastoma cells via the WNT/ ?-catenin pathway.

Wnt5A/Ryk signaling might provide novel therapeutic strategies to prevent capillary leakage in systemic inflammation and septic shock.
Title: Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells.

results corroborated previous findings of Ryk-mediated Wnt5a effect, and suggested a role for Ror2 in the Wnt5a machinery in glioblastoma
Title: Wnt5a, Ryk and Ror2 expression in glioblastoma subgroups.

Early treatment with HGF/IgG complexes after myocardial ischaemia with reperfusion may rescue tissue through vasoprotection conferred by c-Met and RYK signalling
Title: Human epicardial cell-conditioned medium contains HGF/IgG complexes that phosphorylate RYK and protect against vascular injury.

Ryk-intracellular domain pathway may impair FOXO protective activity in mutant polyglutamine neurons, suggesting neurons unable to efficiently maintain function and resist disease from the earliest phases of the pathogenic process in Huntington disease
Title: The Wnt receptor Ryk reduces neuronal and cell survival capacity by repressing FOXO activity during the early phases of mutant huntingtin pathogenicity.

The following RYK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RYK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu94483	XM_017007016.1 Latest version!	Homo sapiens receptor-like tyrosine kinase (RYK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu18752	NM_002958.3 Latest version!	Homo sapiens receptor like tyrosine kinase (RYK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$356.30 $509.00
OHu30020	NM_001005861.2 Latest version!	Homo sapiens receptor like tyrosine kinase (RYK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$363.30 $519.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RYK ( XM_017007016.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862505.1 Homo sapiens receptor-like tyrosine kinase (RYK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu94483
Clone ID Related Accession (Same CDS sequence)	XM_017007016.1
Accession Version	XM_017007016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase RYK isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAG TTCTTCTGCC CTTTCCTGTC CTATTAACAA TTATTTATTA CCTGCCAGGC 
AAGCTAACAG TGGTGACCAG AAAGCTCACA TCCAGTTATC CAACAGCATT TAAGAGACAT 
CTGCTGCTTC TCTCTCTGCA TAGGGGCCAC TGGGATTACC ATTTATTGAG TGTTTTCTGT 
TTACCAGTAC TGTACCCCAG CATTAGTGCC AGCAGTAGTT CCCAAGGGCT GTCTCAGCCA 
TCCACCCAGA CGACTCAGTA TCTGAGAGCA GACACGCCCA ACAATGCAAC TCCTATCACC 
AGTTATCCTA CCTTGCGGAT AGAGAAGAAC GACTTGAGAA GTGTCACTCT TTTGGAGGCC 
AAAGGCAAGG TGAAGGATAT AGCAATATCC AGAGAGAGGA TAACTCTAAA AGATGTACTC 
CAAGAAGGTA CTTTTGGGCG TATTTTCCAT GGGATTTTAA TAGATGAAAA AGATCCAAAT 
AAAGAAAAAC AAGCATTTGT CAAAACAGTT AAAGATCAAG CTTCTGAAAT TCAGGTGACA 
ATGATGCTCA CTGAAAGTTG TAAGCTGCGA GGTCTTCATC ACAGAAATCT TCTTCCTATT 
ACTCATGTGT GTATAGAAGA AGGAGAAAAG CCCATGGTGA TATTGCCTTA CATGAATTGG 
GGGAATCTTA AATTGTTTTT ACGACAGTGC AAGTTAGTAG AGGCCAATAA TCCACAGGCA 
ATTTCTCAGC AAGACCTGGT ACACATGGCT ATTCAGATTG CCTGTGGAAT GAGCTACCTG 
GCCAGAAGGG AAGTCATCCA CAAAGACCTG GCTGCCAGGA ACTGTGTCAT TGATGACACA 
CTTCAAGTTA AGATCACAGA CAATGCCCTC TCCAGAGACT TGTTCCCCAT GGACTATCAC 
TGTCTGGGGG ACAATGAAAA CAGGCCAGTT CGTTGGATGG CTCTTGAAAG TCTGGTTAAT 
AACGAGTTCT CTAGCGCTAG TGATGTGTGG GCCTTTGGAG TGACGCTGTG GGAACTCATG 
ACTCTGGGCC AGACTCCCTA CGTGGACATT GACCCCTTCG AGATGGCCGC ATACCTGAAA 
GATGGTTACC GAATAGCCCA GCCAATCAAC TGTCCTGATG AATTATTTGC TGTGATGGCC 
TGTTGCTGGG CCTTAGATCC AGAGGAGAGG CCCAAGTTTC AGCAGCTGGT ACAGTGCCTA 
ACAGAGTTTC ATGCAGCCCT GGGGGCCTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862505.1
CDS	185..1420
Translation	MDKVLLPFPVLLTIIYYLPGKLTVVTRKLTSSYPTAFKRHLLLLSLHRGHWDYHLLSVFCLPVLYPSISASSSSQGLSQPSTQTTQYLRADTPNNATPITSYPTLRIEKNDLRSVTLLEAKGKVKDIAISRERITLKDVLQEGTFGRIFHGILIDEKDPNKEKQAFVKTVKDQASEIQVTMMLTESCKLRGLHHRNLLPITHVCIEEGEKPMVILPYMNWGNLKLFLRQCKLVEANNPQAISQQDLVHMAIQIACGMSYLARREVIHKDLAARNCVIDDTLQVKITDNALSRDLFPMDYHCLGDNENRPVRWMALESLVNNEFSSASDVWAFGVTLWELMTLGQTPYVDIDPFEMAAYLKDGYRIAQPINCPDELFAVMACCWALDPEERPKFQQLVQCLTEFHAALGAYV

Target ORF information:

RefSeq Version	XM_017007016.1
Organism	Homo sapiens(human)
Definition	Homo sapiens receptor-like tyrosine kinase (RYK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007016.1

ORF Insert Sequence:

ATGGACAAAG TTCTTCTGCC CTTTCCTGTC CTATTAACAA TTATTTATTA CCTGCCAGGC 
AAGCTAACAG TGGTGACCAG AAAGCTCACA TCCAGTTATC CAACAGCATT TAAGAGACAT 
CTGCTGCTTC TCTCTCTGCA TAGGGGCCAC TGGGATTACC ATTTATTGAG TGTTTTCTGT 
TTACCAGTAC TGTACCCCAG CATTAGTGCC AGCAGTAGTT CCCAAGGGCT GTCTCAGCCA 
TCCACCCAGA CGACTCAGTA TCTGAGAGCA GACACGCCCA ACAATGCAAC TCCTATCACC 
AGTTATCCTA CCTTGCGGAT AGAGAAGAAC GACTTGAGAA GTGTCACTCT TTTGGAGGCC 
AAAGGCAAGG TGAAGGATAT AGCAATATCC AGAGAGAGGA TAACTCTAAA AGATGTACTC 
CAAGAAGGTA CTTTTGGGCG TATTTTCCAT GGGATTTTAA TAGATGAAAA AGATCCAAAT 
AAAGAAAAAC AAGCATTTGT CAAAACAGTT AAAGATCAAG CTTCTGAAAT TCAGGTGACA 
ATGATGCTCA CTGAAAGTTG TAAGCTGCGA GGTCTTCATC ACAGAAATCT TCTTCCTATT 
ACTCATGTGT GTATAGAAGA AGGAGAAAAG CCCATGGTGA TATTGCCTTA CATGAATTGG 
GGGAATCTTA AATTGTTTTT ACGACAGTGC AAGTTAGTAG AGGCCAATAA TCCACAGGCA 
ATTTCTCAGC AAGACCTGGT ACACATGGCT ATTCAGATTG CCTGTGGAAT GAGCTACCTG 
GCCAGAAGGG AAGTCATCCA CAAAGACCTG GCTGCCAGGA ACTGTGTCAT TGATGACACA 
CTTCAAGTTA AGATCACAGA CAATGCCCTC TCCAGAGACT TGTTCCCCAT GGACTATCAC 
TGTCTGGGGG ACAATGAAAA CAGGCCAGTT CGTTGGATGG CTCTTGAAAG TCTGGTTAAT 
AACGAGTTCT CTAGCGCTAG TGATGTGTGG GCCTTTGGAG TGACGCTGTG GGAACTCATG 
ACTCTGGGCC AGACTCCCTA CGTGGACATT GACCCCTTCG AGATGGCCGC ATACCTGAAA 
GATGGTTACC GAATAGCCCA GCCAATCAAC TGTCCTGATG AATTATTTGC TGTGATGGCC 
TGTTGCTGGG CCTTAGATCC AGAGGAGAGG CCCAAGTTTC AGCAGCTGGT ACAGTGCCTA 
ACAGAGTTTC ATGCAGCCCT GGGGGCCTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RYK ( NM_002958.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002949.2 Homo sapiens receptor like tyrosine kinase (RYK), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18752
Clone ID Related Accession (Same CDS sequence)	NM_002958.3
Accession Version	NM_002958.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase RYK isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X69970.1, CN309980.1, BC021700.2 and AB209405.1. This sequence is a reference standard in the RefSeqGene project. On Oct 27, 2006 this sequence version replaced NM_002958.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X69970.1, SRR1803611.83878.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGTGGGG CGGCGCGGCT GGGGCGGCCG GGCCGGAGTT GCCTCCCGGG GGCCCGCGGC 
CTGAGGGCCC CGCCGCCGCC GCCGCTGCTG CTTCTGCTTG CGCTGTTGCC GCTGCTGCCC 
GCGCCTGGCG CTGCCGCCGC CCCCGCCCCG CGGCCCCCGG AGCTGCAGTC GGCTTCCGCG 
GGGCCCAGCG TGAGTCTCTA CCTGAGCGAG GACGAGGTGC GCCGGCTGAT CGGTCTTGAT 
GCAGAACTTT ATTATGTGAG AAATGACCTT ATTAGTCACT ACGCTCTATC CTTTAGTCTG 
TTAGTACCCA GTGAGACAAA TTTCCTGCAC TTCACCTGGC ATGCGAAGTC CAAGGTTGAA 
TATAAGCTGG GATTCCAAGT GGACAATGTT TTGGCAATGG ATATGCCCCA GGTCAACATT 
TCTGTTCAGG GGGAAGTTCC ACGCACTTTA TCAGTGTTTC GGGTAGAGCT TTCCTGTACT 
GGCAAAGTAG ATTCTGAAGT TATGATACTA ATGCAGCTCA ACTTGACAGT AAATTCTTCA 
AAAAATTTTA CCGTCTTAAA TTTTAAACGA AGGAAAATGT GCTACAAAAA ACTTGAAGAA 
GTAAAAACTT CAGCCTTGGA CAAAAACACT AGCAGAACTA TTTATGATCC TGTACATGCA 
GCTCCAACCA CTTCTACGCG TGTGTTTTAT ATTAGTGTAG GGGTTTGTTG TGCAGTAATA 
TTTCTCGTAG CAATAATATT AGCTGTTTTG CACCTTCATA GTATGAAAAG GATTGAACTG 
GATGACAGCA TTAGTGCCAG CAGTAGTTCC CAAGGGCTGT CTCAGCCATC CACCCAGACG 
ACTCAGTATC TGAGAGCAGA CACGCCCAAC AATGCAACTC CTATCACCAG TTATCCTACC 
TTGCGGATAG AGAAGAACGA CTTGAGAAGT GTCACTCTTT TGGAGGCCAA AGGCAAGGTG 
AAGGATATAG CAATATCCAG AGAGAGGATA ACTCTAAAAG ATGTACTCCA AGAAGGTACT 
TTTGGGCGTA TTTTCCATGG GATTTTAATA GATGAAAAAG ATCCAAATAA AGAAAAACAA 
GCATTTGTCA AAACAGTTAA AGATCAAGCT TCTGAAATTC AGGTGACAAT GATGCTCACT 
GAAAGTTGTA AGCTGCGAGG TCTTCATCAC AGAAATCTTC TTCCTATTAC TCATGTGTGT 
ATAGAAGAAG GAGAAAAGCC CATGGTGATA TTGCCTTACA TGAATTGGGG GAATCTTAAA 
TTGTTTTTAC GACAGTGCAA GTTAGTAGAG GCCAATAATC CACAGGCAAT TTCTCAGCAA 
GACCTGGTAC ACATGGCTAT TCAGATTGCC TGTGGAATGA GCTACCTGGC CAGAAGGGAA 
GTCATCCACA AAGACCTGGC TGCCAGGAAC TGTGTCATTG ATGACACACT TCAAGTTAAG 
ATCACAGACA ATGCCCTCTC CAGAGACTTG TTCCCCATGG ACTATCACTG TCTGGGGGAC 
AATGAAAACA GGCCAGTTCG TTGGATGGCT CTTGAAAGTC TGGTTAATAA CGAGTTCTCT 
AGCGCTAGTG ATGTGTGGGC CTTTGGAGTG ACGCTGTGGG AACTCATGAC TCTGGGCCAG 
ACTCCCTACG TGGACATTGA CCCCTTCGAG ATGGCCGCAT ACCTGAAAGA TGGTTACCGA 
ATAGCCCAGC CAATCAACTG TCCTGATGAA TTATTTGCTG TGATGGCCTG TTGCTGGGCC 
TTAGATCCAG AGGAGAGGCC CAAGTTTCAG CAGCTGGTAC AGTGCCTAAC AGAGTTTCAT 
GCAGCCCTGG GGGCCTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002949.2
CDS	91..1914
Translation	MRGAARLGRPGRSCLPGARGLRAPPPPPLLLLLALLPLLPAPGAAAAPAPRPPELQSASAGPSVSLYLSEDEVRRLIGLDAELYYVRNDLISHYALSFSLLVPSETNFLHFTWHAKSKVEYKLGFQVDNVLAMDMPQVNISVQGEVPRTLSVFRVELSCTGKVDSEVMILMQLNLTVNSSKNFTVLNFKRRKMCYKKLEEVKTSALDKNTSRTIYDPVHAAPTTSTRVFYISVGVCCAVIFLVAIILAVLHLHSMKRIELDDSISASSSSQGLSQPSTQTTQYLRADTPNNATPITSYPTLRIEKNDLRSVTLLEAKGKVKDIAISRERITLKDVLQEGTFGRIFHGILIDEKDPNKEKQAFVKTVKDQASEIQVTMMLTESCKLRGLHHRNLLPITHVCIEEGEKPMVILPYMNWGNLKLFLRQCKLVEANNPQAISQQDLVHMAIQIACGMSYLARREVIHKDLAARNCVIDDTLQVKITDNALSRDLFPMDYHCLGDNENRPVRWMALESLVNNEFSSASDVWAFGVTLWELMTLGQTPYVDIDPFEMAAYLKDGYRIAQPINCPDELFAVMACCWALDPEERPKFQQLVQCLTEFHAALGAYV

Target ORF information:

RefSeq Version	NM_002958.3
Organism	Homo sapiens(human)
Definition	Homo sapiens receptor like tyrosine kinase (RYK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002958.3

ORF Insert Sequence:

ATGCGTGGGG CGGCGCGGCT GGGGCGGCCG GGCCGGAGTT GCCTCCCGGG GGCCCGCGGC 
CTGAGGGCCC CGCCGCCGCC GCCGCTGCTG CTTCTGCTTG CGCTGTTGCC GCTGCTGCCC 
GCGCCTGGCG CTGCCGCCGC CCCCGCCCCG CGGCCCCCGG AGCTGCAGTC GGCTTCCGCG 
GGGCCCAGCG TGAGTCTCTA CCTGAGCGAG GACGAGGTGC GCCGGCTGAT CGGTCTTGAT 
GCAGAACTTT ATTATGTGAG AAATGACCTT ATTAGTCACT ACGCTCTATC CTTTAGTCTG 
TTAGTACCCA GTGAGACAAA TTTCCTGCAC TTCACCTGGC ATGCGAAGTC CAAGGTTGAA 
TATAAGCTGG GATTCCAAGT GGACAATGTT TTGGCAATGG ATATGCCCCA GGTCAACATT 
TCTGTTCAGG GGGAAGTTCC ACGCACTTTA TCAGTGTTTC GGGTAGAGCT TTCCTGTACT 
GGCAAAGTAG ATTCTGAAGT TATGATACTA ATGCAGCTCA ACTTGACAGT AAATTCTTCA 
AAAAATTTTA CCGTCTTAAA TTTTAAACGA AGGAAAATGT GCTACAAAAA ACTTGAAGAA 
GTAAAAACTT CAGCCTTGGA CAAAAACACT AGCAGAACTA TTTATGATCC TGTACATGCA 
GCTCCAACCA CTTCTACGCG TGTGTTTTAT ATTAGTGTAG GGGTTTGTTG TGCAGTAATA 
TTTCTCGTAG CAATAATATT AGCTGTTTTG CACCTTCATA GTATGAAAAG GATTGAACTG 
GATGACAGCA TTAGTGCCAG CAGTAGTTCC CAAGGGCTGT CTCAGCCATC CACCCAGACG 
ACTCAGTATC TGAGAGCAGA CACGCCCAAC AATGCAACTC CTATCACCAG TTATCCTACC 
TTGCGGATAG AGAAGAACGA CTTGAGAAGT GTCACTCTTT TGGAGGCCAA AGGCAAGGTG 
AAGGATATAG CAATATCCAG AGAGAGGATA ACTCTAAAAG ATGTACTCCA AGAAGGTACT 
TTTGGGCGTA TTTTCCATGG GATTTTAATA GATGAAAAAG ATCCAAATAA AGAAAAACAA 
GCATTTGTCA AAACAGTTAA AGATCAAGCT TCTGAAATTC AGGTGACAAT GATGCTCACT 
GAAAGTTGTA AGCTGCGAGG TCTTCATCAC AGAAATCTTC TTCCTATTAC TCATGTGTGT 
ATAGAAGAAG GAGAAAAGCC CATGGTGATA TTGCCTTACA TGAATTGGGG GAATCTTAAA 
TTGTTTTTAC GACAGTGCAA GTTAGTAGAG GCCAATAATC CACAGGCAAT TTCTCAGCAA 
GACCTGGTAC ACATGGCTAT TCAGATTGCC TGTGGAATGA GCTACCTGGC CAGAAGGGAA 
GTCATCCACA AAGACCTGGC TGCCAGGAAC TGTGTCATTG ATGACACACT TCAAGTTAAG 
ATCACAGACA ATGCCCTCTC CAGAGACTTG TTCCCCATGG ACTATCACTG TCTGGGGGAC 
AATGAAAACA GGCCAGTTCG TTGGATGGCT CTTGAAAGTC TGGTTAATAA CGAGTTCTCT 
AGCGCTAGTG ATGTGTGGGC CTTTGGAGTG ACGCTGTGGG AACTCATGAC TCTGGGCCAG 
ACTCCCTACG TGGACATTGA CCCCTTCGAG ATGGCCGCAT ACCTGAAAGA TGGTTACCGA 
ATAGCCCAGC CAATCAACTG TCCTGATGAA TTATTTGCTG TGATGGCCTG TTGCTGGGCC 
TTAGATCCAG AGGAGAGGCC CAAGTTTCAG CAGCTGGTAC AGTGCCTAAC AGAGTTTCAT 
GCAGCCCTGG GGGCCTACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RYK ( NM_001005861.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005861.1 Homo sapiens receptor like tyrosine kinase (RYK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30020
Clone ID Related Accession (Same CDS sequence)	NM_001005861.2
Accession Version	NM_001005861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase RYK isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X69970.1, CN309980.1, BC021700.2 and AB209405.1. On Oct 27, 2006 this sequence version replaced NM_001005861.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGTGGGG CGGCGCGGCT GGGGCGGCCG GGCCGGAGTT GCCTCCCGGG GGCCCGCGGC 
CTGAGGGCCC CGCCGCCGCC GCCGCTGCTG CTTCTGCTTG CGCTGTTGCC GCTGCTGCCC 
GCGCCTGGCG CTGCCGCCGC CCCCGCCCCG CGGCCCCCGG AGCTGCAGTC GGCTTCCGCG 
GGGCCCAGCG TGAGTCTCTA CCTGAGCGAG GACGAGGTGC GCCGGCTGAT CGGTCTTGAT 
GCAGAACTTT ATTATGTGAG AAATGACCTT ATTAGTCACT ACGCTCTATC CTTTAGTCTG 
TTAGTACCCA GTGAGACAAA TTTCCTGCAC TTCACCTGGC ATGCGAAGTC CAAGGTTGAA 
TATAAGCTGG GATTCCAAGT GGACAATGTT TTGGCAATGG ATATGCCCCA GGTCAACATT 
TCTGTTCAGG GGGAAGTTCC ACGCACTTTA TCAGTGTTTC GGGTAGAGCT TTCCTGTACT 
GGCAAAGTAG ATTCTGAAGT TATGATACTA ATGCAGCTCA ACTTGACAGT AAATTCTTCA 
AAAAATTTTA CCGTCTTAAA TTTTAAACGA AGGAAAATGT GCTACAAAAA ACTTGAAGAA 
GTAAAAACTT CAGCCTTGGA CAAAAACACT AGCAGAACTA TTTATGATCC TGTACATGCA 
GCTCCAACCA CTTCTACGCG TGTGTTTTAT ATTAGTGTAG GGGTTTGTTG TGCAGTAATA 
TTTCTCGTAG CAATAATATT AGCTGTTTTG CACCTTCATA GTATGAAAAG GATTGAACTG 
GATGACAGCA TTAGTGCCAG CAGTAGTTCC CAAGGGCTGT CTCAGCCATC CACCCAGACG 
ACTCAGTATC TGAGAGCAGA CACGCCCAAC AATGCAACTC CTATCACCAG CTCCTTAGGT 
TATCCTACCT TGCGGATAGA GAAGAACGAC TTGAGAAGTG TCACTCTTTT GGAGGCCAAA 
GGCAAGGTGA AGGATATAGC AATATCCAGA GAGAGGATAA CTCTAAAAGA TGTACTCCAA 
GAAGGTACTT TTGGGCGTAT TTTCCATGGG ATTTTAATAG ATGAAAAAGA TCCAAATAAA 
GAAAAACAAG CATTTGTCAA AACAGTTAAA GATCAAGCTT CTGAAATTCA GGTGACAATG 
ATGCTCACTG AAAGTTGTAA GCTGCGAGGT CTTCATCACA GAAATCTTCT TCCTATTACT 
CATGTGTGTA TAGAAGAAGG AGAAAAGCCC ATGGTGATAT TGCCTTACAT GAATTGGGGG 
AATCTTAAAT TGTTTTTACG ACAGTGCAAG TTAGTAGAGG CCAATAATCC ACAGGCAATT 
TCTCAGCAAG ACCTGGTACA CATGGCTATT CAGATTGCCT GTGGAATGAG CTACCTGGCC 
AGAAGGGAAG TCATCCACAA AGACCTGGCT GCCAGGAACT GTGTCATTGA TGACACACTT 
CAAGTTAAGA TCACAGACAA TGCCCTCTCC AGAGACTTGT TCCCCATGGA CTATCACTGT 
CTGGGGGACA ATGAAAACAG GCCAGTTCGT TGGATGGCTC TTGAAAGTCT GGTTAATAAC 
GAGTTCTCTA GCGCTAGTGA TGTGTGGGCC TTTGGAGTGA CGCTGTGGGA ACTCATGACT 
CTGGGCCAGA CTCCCTACGT GGACATTGAC CCCTTCGAGA TGGCCGCATA CCTGAAAGAT 
GGTTACCGAA TAGCCCAGCC AATCAACTGT CCTGATGAAT TATTTGCTGT GATGGCCTGT 
TGCTGGGCCT TAGATCCAGA GGAGAGGCCC AAGTTTCAGC AGCTGGTACA GTGCCTAACA 
GAGTTTCATG CAGCCCTGGG GGCCTACGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005861.1
CDS	91..1923
Translation	MRGAARLGRPGRSCLPGARGLRAPPPPPLLLLLALLPLLPAPGAAAAPAPRPPELQSASAGPSVSLYLSEDEVRRLIGLDAELYYVRNDLISHYALSFSLLVPSETNFLHFTWHAKSKVEYKLGFQVDNVLAMDMPQVNISVQGEVPRTLSVFRVELSCTGKVDSEVMILMQLNLTVNSSKNFTVLNFKRRKMCYKKLEEVKTSALDKNTSRTIYDPVHAAPTTSTRVFYISVGVCCAVIFLVAIILAVLHLHSMKRIELDDSISASSSSQGLSQPSTQTTQYLRADTPNNATPITSSLGYPTLRIEKNDLRSVTLLEAKGKVKDIAISRERITLKDVLQEGTFGRIFHGILIDEKDPNKEKQAFVKTVKDQASEIQVTMMLTESCKLRGLHHRNLLPITHVCIEEGEKPMVILPYMNWGNLKLFLRQCKLVEANNPQAISQQDLVHMAIQIACGMSYLARREVIHKDLAARNCVIDDTLQVKITDNALSRDLFPMDYHCLGDNENRPVRWMALESLVNNEFSSASDVWAFGVTLWELMTLGQTPYVDIDPFEMAAYLKDGYRIAQPINCPDELFAVMACCWALDPEERPKFQQLVQCLTEFHAALGAYV

Target ORF information:

RefSeq Version	NM_001005861.2
Organism	Homo sapiens(human)
Definition	Homo sapiens receptor like tyrosine kinase (RYK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005861.2

ORF Insert Sequence:

ATGCGTGGGG CGGCGCGGCT GGGGCGGCCG GGCCGGAGTT GCCTCCCGGG GGCCCGCGGC 
CTGAGGGCCC CGCCGCCGCC GCCGCTGCTG CTTCTGCTTG CGCTGTTGCC GCTGCTGCCC 
GCGCCTGGCG CTGCCGCCGC CCCCGCCCCG CGGCCCCCGG AGCTGCAGTC GGCTTCCGCG 
GGGCCCAGCG TGAGTCTCTA CCTGAGCGAG GACGAGGTGC GCCGGCTGAT CGGTCTTGAT 
GCAGAACTTT ATTATGTGAG AAATGACCTT ATTAGTCACT ACGCTCTATC CTTTAGTCTG 
TTAGTACCCA GTGAGACAAA TTTCCTGCAC TTCACCTGGC ATGCGAAGTC CAAGGTTGAA 
TATAAGCTGG GATTCCAAGT GGACAATGTT TTGGCAATGG ATATGCCCCA GGTCAACATT 
TCTGTTCAGG GGGAAGTTCC ACGCACTTTA TCAGTGTTTC GGGTAGAGCT TTCCTGTACT 
GGCAAAGTAG ATTCTGAAGT TATGATACTA ATGCAGCTCA ACTTGACAGT AAATTCTTCA 
AAAAATTTTA CCGTCTTAAA TTTTAAACGA AGGAAAATGT GCTACAAAAA ACTTGAAGAA 
GTAAAAACTT CAGCCTTGGA CAAAAACACT AGCAGAACTA TTTATGATCC TGTACATGCA 
GCTCCAACCA CTTCTACGCG TGTGTTTTAT ATTAGTGTAG GGGTTTGTTG TGCAGTAATA 
TTTCTCGTAG CAATAATATT AGCTGTTTTG CACCTTCATA GTATGAAAAG GATTGAACTG 
GATGACAGCA TTAGTGCCAG CAGTAGTTCC CAAGGGCTGT CTCAGCCATC CACCCAGACG 
ACTCAGTATC TGAGAGCAGA CACGCCCAAC AATGCAACTC CTATCACCAG CTCCTTAGGT 
TATCCTACCT TGCGGATAGA GAAGAACGAC TTGAGAAGTG TCACTCTTTT GGAGGCCAAA 
GGCAAGGTGA AGGATATAGC AATATCCAGA GAGAGGATAA CTCTAAAAGA TGTACTCCAA 
GAAGGTACTT TTGGGCGTAT TTTCCATGGG ATTTTAATAG ATGAAAAAGA TCCAAATAAA 
GAAAAACAAG CATTTGTCAA AACAGTTAAA GATCAAGCTT CTGAAATTCA GGTGACAATG 
ATGCTCACTG AAAGTTGTAA GCTGCGAGGT CTTCATCACA GAAATCTTCT TCCTATTACT 
CATGTGTGTA TAGAAGAAGG AGAAAAGCCC ATGGTGATAT TGCCTTACAT GAATTGGGGG 
AATCTTAAAT TGTTTTTACG ACAGTGCAAG TTAGTAGAGG CCAATAATCC ACAGGCAATT 
TCTCAGCAAG ACCTGGTACA CATGGCTATT CAGATTGCCT GTGGAATGAG CTACCTGGCC 
AGAAGGGAAG TCATCCACAA AGACCTGGCT GCCAGGAACT GTGTCATTGA TGACACACTT 
CAAGTTAAGA TCACAGACAA TGCCCTCTCC AGAGACTTGT TCCCCATGGA CTATCACTGT 
CTGGGGGACA ATGAAAACAG GCCAGTTCGT TGGATGGCTC TTGAAAGTCT GGTTAATAAC 
GAGTTCTCTA GCGCTAGTGA TGTGTGGGCC TTTGGAGTGA CGCTGTGGGA ACTCATGACT 
CTGGGCCAGA CTCCCTACGT GGACATTGAC CCCTTCGAGA TGGCCGCATA CCTGAAAGAT 
GGTTACCGAA TAGCCCAGCC AATCAACTGT CCTGATGAAT TATTTGCTGT GATGGCCTGT 
TGCTGGGCCT TAGATCCAGA GGAGAGGCCC AAGTTTCAGC AGCTGGTACA GTGCCTAACA 
GAGTTTCATG CAGCCCTGGG GGCCTACGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RYK promotes the stemness of glioblastoma cells via the WNT/ ?-catenin pathway.
Oncotarget8(8)13476-13487(2017 Feb)
Adamo A,Fiore D,De Martino F,Roscigno G,Affinito A,Donnarumma E,Puoti I,Ricci Vitiani L,Pallini R,Quintavalle C,Condorelli G

Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells.
Cell adhesion & migration11(1)24-38(2017 Jan)
Skaria T,Bachli E,Schoedon G

Wnt5a, Ryk and Ror2 expression in glioblastoma subgroups.
Pathology, research and practice211(12)963-72(2015 Dec)
Kim Y,Hong M,Do IG,Ha SY,Lee D,Suh YL

Human epicardial cell-conditioned medium contains HGF/IgG complexes that phosphorylate RYK and protect against vascular injury.
Cardiovascular research107(2)277-86(2015 Jul)
Rao KS,Aronshtam A,McElory-Yaggy KL,Bakondi B,VanBuren P,Sobel BE,Spees JL

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RYK cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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