RPS29 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPS29
Entrez Gene ID	6235
Full Name	ribosomal protein S29
Synonyms	DBA13,S29
General protein information	Preferred Names ribosomal protein S29 Names 40S ribosomal protein S29 small ribosomal subunit protein uS14
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q21.3
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit and a member of the S14P family of ribosomal proteins. The protein, which contains a C2-C2 zinc finger-like domain that can bind to zinc, can enhance the tumor suppressor activity of Ras-related protein 1A (KREV1). It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2013].
Disorder MIM:	603633
Disorder Html:	Diamond-Blackfan anemia 13, 615909 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001030001.4	NP_001025172.1	40S ribosomal protein S29 isoform 2
NM_001032.4	NP_001023.1	40S ribosomal protein S29 isoform 1
NM_001030001.2	NP_001025172.1	40S ribosomal protein S29 isoform 2
NM_001351375.1	NP_001338304.1	40S ribosomal protein S29 isoform 3
NM_001030001.3	NP_001025172.1	40S ribosomal protein S29 isoform 2
NM_001032.5	NP_001023.1	40S ribosomal protein S29 isoform 1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	rps29	NP_001016766.1
	LOC700392	XP_001082945.2
	LOC702065	XP_001093477.2
Canis lupus familiaris (dog)	RPS29	XP_853223.1
Rattus norvegicus (Norway rat)	Rps29	NP_037008.1
Homo sapiens (human)	RPS29	NP_001025172.1
Gallus gallus (chicken)	RPS29	NP_001264809.1
Pan troglodytes (chimpanzee)	RPS29	XP_003314360.1
	LOC693820	XP_001082271.2
Bos taurus (cattle)	RPS29	NP_777229.1
Mus musculus (house mouse)	Rps29	NP_033119.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural snapshots of actively translating human ribosomes.
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, B?rger J, Mielke T, Scheerer P, Spahn CM
Cell161(4)845-57(2015 May)

Whole-exome sequencing and functional studies identify RPS29 as a novel gene mutated in multicase Diamond-Blackfan anemia families.
Mirabello L, Macari ER, Jessop L, Ellis SR, Myers T, Giri N, Taylor AM, McGrath KE, Humphries JM, Ballew BJ, Yeager M, Boland JF, He J, Hicks BD, Burdett L, Alter BP, Zon L, Savage SA
Blood124(1)24-32(2014 Jul)

Genome-wide association study identifies new susceptibility loci for posttraumatic stress disorder.
Xie P, Kranzler HR, Yang C, Zhao H, Farrer LA, Gelernter J
Biological psychiatry74(9)656-63(2013 Nov)

Structures of the human and Drosophila 80S ribosome.
Anger AM, Armache JP, Berninghausen O, Habeck M, Subklewe M, Wilson DN, Beckmann R
Nature497(7447)80-5(2013 May)

Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women.
Fox CS, Liu Y, White CC, Feitosa M, Smith AV, Heard-Costa N, Lohman K, , , , Johnson AD, Foster MC, Greenawalt DM, Griffin P, Ding J, Newman AB, Tylavsky F, Miljkovic I, Kritchevsky SB, Launer L, Garcia M, Eiriksdottir G, Carr JJ, Gudnason V, Harris TB, Cupples LA, Borecki IB
PLoS genetics8(5)e1002695(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We uncovered a novel Diamond-Blackfan anemia causative gene, RPS29
Title: Whole-exome sequencing and functional studies identify RPS29 as a novel gene mutated in multicase Diamond-Blackfan anemia families.

The content of protein S29 in ribosomes of lung cancer cell line A549 was distinctly low.
Title: Low content of protein S29 in ribosomes of human lung cancer cell line a549: detected by two-dimensional electrophoresis.

The following RPS29 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPS29 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02566	NM_001032.5 Latest version!	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu08400	NM_001030001.4 Latest version!	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu103279	NM_001351375.1 Latest version!	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu08400	NM_001030001.2	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu08400	NM_001030001.3	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu02566	NM_001032.4	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPS29 ( NM_001032.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001023.1 Homo sapiens ribosomal protein S29 (RPS29), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02566
Clone ID Related Accession (Same CDS sequence)	NM_001032.4 , NM_001032.5
Accession Version	NM_001032.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	40S ribosomal protein S29 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BU942244.1 and BC035313.1. This sequence is a reference standard in the RefSeqGene project. On Mar 8, 2013 this sequence version replaced NM_001032.3. Transcript Variant: This variant (1) is the predominant transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD172695.1, BU942244.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121

ATGGGTCACC AGCAGCTGTA CTGGAGCCAC CCGCGAAAAT TCGGCCAGGG TTCTCGCTCT 
TGTCGTGTCT GTTCAAACCG GCACGGTCTG ATCCGGAAAT ATGGCCTCAA TATGTGCCGC 
CAGTGTTTCC GTCAGTACGC GAAGGATATC GGTTTCATTA AGTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001023.1
CDS	71..241
Translation	MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKLD

Target ORF information:

RefSeq Version	NM_001032.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032.4

ORF Insert Sequence:

1
61
121

ATGGGTCACC AGCAGCTGTA CTGGAGCCAC CCGCGAAAAT TCGGCCAGGG TTCTCGCTCT 
TGTCGTGTCT GTTCAAACCG GCACGGTCTG ATCCGGAAAT ATGGCCTCAA TATGTGCCGC 
CAGTGTTTCC GTCAGTACGC GAAGGATATC GGTTTCATTA AGTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS29 ( NM_001032.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001023.1 Homo sapiens ribosomal protein S29 (RPS29), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02566
Clone ID Related Accession (Same CDS sequence)	NM_001032.4 , NM_001032.5
Accession Version	NM_001032.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-29
Organism	Homo sapiens(human)
Product	40S ribosomal protein S29 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BU942244.1 and BC035313.1. On Nov 30, 2019 this sequence version replaced NM_001032.4. Transcript Variant: This variant (1) is the predominant transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB110739.1, CD249531.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121

ATGGGTCACC AGCAGCTGTA CTGGAGCCAC CCGCGAAAAT TCGGCCAGGG TTCTCGCTCT 
TGTCGTGTCT GTTCAAACCG GCACGGTCTG ATCCGGAAAT ATGGCCTCAA TATGTGCCGC 
CAGTGTTTCC GTCAGTACGC GAAGGATATC GGTTTCATTA AGTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001023.1
CDS	35..205
Translation	MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKLD

Target ORF information:

RefSeq Version	NM_001032.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032.5

ORF Insert Sequence:

1
61
121

ATGGGTCACC AGCAGCTGTA CTGGAGCCAC CCGCGAAAAT TCGGCCAGGG TTCTCGCTCT 
TGTCGTGTCT GTTCAAACCG GCACGGTCTG ATCCGGAAAT ATGGCCTCAA TATGTGCCGC 
CAGTGTTTCC GTCAGTACGC GAAGGATATC GGTTTCATTA AGTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS29 ( NM_001030001.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025172.1 Homo sapiens ribosomal protein S29 (RPS29), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08400
Clone ID Related Accession (Same CDS sequence)	NM_001030001.4 , NM_001030001.2 , NM_001030001.3
Accession Version	NM_001030001.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	40S ribosomal protein S29 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU942244.1, BC015974.1 and AL139099.3. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001030001.3. Transcript Variant: This variant (2) utilizes an alternate 3'-terminal exon, compared to variant 1, resulting in a longer isoform (2) with a unique C-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.103105.1, SRR1163655.714687.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCACC AGCAGCTGTA CTGGAGCCAC CCGCGAAAAT TCGGCCAGGG TTCTCGCTCT 
TGTCGTGTCT GTTCAAACCG GCACGGTCTG ATCCGGAAAT ATGGCCTCAA TATGTGCCGC 
CAGTGTTTCC GTCAGTACGC GAAGGATATC GGTTTCATTA AGAAAGACCT GAGCTGTCTT 
CCTTGGCACT GCCTATGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025172.1
CDS	35..238
Translation	MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKKDLSCLPWHCLWR

Target ORF information:

RefSeq Version	NM_001030001.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030001.4

ORF Insert Sequence:

ATGGGTCACC AGCAGCTGTA CTGGAGCCAC CCGCGAAAAT TCGGCCAGGG TTCTCGCTCT 
TGTCGTGTCT GTTCAAACCG GCACGGTCTG ATCCGGAAAT ATGGCCTCAA TATGTGCCGC 
CAGTGTTTCC GTCAGTACGC GAAGGATATC GGTTTCATTA AGAAAGACCT GAGCTGTCTT 
CCTTGGCACT GCCTATGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS29 ( NM_001030001.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025172.1 Homo sapiens ribosomal protein S29 (RPS29), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08400
Clone ID Related Accession (Same CDS sequence)	NM_001030001.4 , NM_001030001.2 , NM_001030001.3
Accession Version	NM_001030001.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-18
Organism	Homo sapiens(Human)
Product	40S ribosomal protein S29 isoform 2
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU942244.1, AK307833.1 and AL139099.3. This sequence is a reference standard in the RefSeqGene project. On Mar 8, 2013 this sequence version replaced NM_001030001.1. Transcript Variant: This variant (2) utilizes an alternate 3'-terminal exon, compared to variant 1, resulting in a longer isoform (2) with a unique C-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK307833.1, BC015974.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END##

ATGGGTCACC AGCAGCTGTA CTGGAGCCAC CCGCGAAAAT TCGGCCAGGG TTCTCGCTCT 
TGTCGTGTCT GTTCAAACCG GCACGGTCTG ATCCGGAAAT ATGGCCTCAA TATGTGCCGC 
CAGTGTTTCC GTCAGTACGC GAAGGATATC GGTTTCATTA AGAAAGACCT GAGCTGTCTT 
CCTTGGCACT GCCTATGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025172.1
CDS	71..274
Translation	MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKKDLSCLPWHCLWR

Target ORF information:

RefSeq Version	NM_001030001.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030001.2

ORF Insert Sequence:

ATGGGTCACC AGCAGCTGTA CTGGAGCCAC CCGCGAAAAT TCGGCCAGGG TTCTCGCTCT 
TGTCGTGTCT GTTCAAACCG GCACGGTCTG ATCCGGAAAT ATGGCCTCAA TATGTGCCGC 
CAGTGTTTCC GTCAGTACGC GAAGGATATC GGTTTCATTA AGAAAGACCT GAGCTGTCTT 
CCTTGGCACT GCCTATGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS29 ( NM_001030001.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025172.1 Homo sapiens ribosomal protein S29 (RPS29), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08400
Clone ID Related Accession (Same CDS sequence)	NM_001030001.4 , NM_001030001.2 , NM_001030001.3
Accession Version	NM_001030001.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	40S ribosomal protein S29 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU942244.1, BC015974.1 and AL139099.3. This sequence is a reference standard in the RefSeqGene project. On May 9, 2017 this sequence version replaced NM_001030001.2. Transcript Variant: This variant (2) utilizes an alternate 3'-terminal exon, compared to variant 1, resulting in a longer isoform (2) with a unique C-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.163529.1, SRR1163655.714687.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCACC AGCAGCTGTA CTGGAGCCAC CCGCGAAAAT TCGGCCAGGG TTCTCGCTCT 
TGTCGTGTCT GTTCAAACCG GCACGGTCTG ATCCGGAAAT ATGGCCTCAA TATGTGCCGC 
CAGTGTTTCC GTCAGTACGC GAAGGATATC GGTTTCATTA AGAAAGACCT GAGCTGTCTT 
CCTTGGCACT GCCTATGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025172.1
CDS	71..274
Translation	MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKKDLSCLPWHCLWR

Target ORF information:

RefSeq Version	NM_001030001.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030001.3

ORF Insert Sequence:

ATGGGTCACC AGCAGCTGTA CTGGAGCCAC CCGCGAAAAT TCGGCCAGGG TTCTCGCTCT 
TGTCGTGTCT GTTCAAACCG GCACGGTCTG ATCCGGAAAT ATGGCCTCAA TATGTGCCGC 
CAGTGTTTCC GTCAGTACGC GAAGGATATC GGTTTCATTA AGAAAGACCT GAGCTGTCTT 
CCTTGGCACT GCCTATGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS29 ( NM_001351375.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338304.1 Homo sapiens ribosomal protein S29 (RPS29), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu103279
Clone ID Related Accession (Same CDS sequence)	NM_001351375.1
Accession Version	NM_001351375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	40S ribosomal protein S29 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY027234.1 and AL139099.3. Transcript Variant: This variant (3) uses an alternate first exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY027234.1, BM673799.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121

ATGGCTGTAT TTGACTTGGC AATTCCTGCT GAACAAATAA TTTTTCATGG CGGTCGTGTC 
TGTTCAAACC GGCACGGTCT GATCCGGAAA TATGGCCTCA ATATGTGCCG CCAGTGTTTC 
CGTCAGTACG CGAAGGATAT CGGTTTCATT AAGTTGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338304.1
CDS	259..420
Translation	MAVFDLAIPAEQIIFHGGRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKLD

Target ORF information:

RefSeq Version	NM_001351375.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S29 (RPS29), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351375.1

ORF Insert Sequence:

1
61
121

ATGGCTGTAT TTGACTTGGC AATTCCTGCT GAACAAATAA TTTTTCATGG CGGTCGTGTC 
TGTTCAAACC GGCACGGTCT GATCCGGAAA TATGGCCTCA ATATGTGCCG CCAGTGTTTC 
CGTCAGTACG CGAAGGATAT CGGTTTCATT AAGTTGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural snapshots of actively translating human ribosomes.
Cell161(4)845-57(2015 May)
Behrmann E,Loerke J,Budkevich TV,Yamamoto K,Schmidt A,Penczek PA,Vos MR,B?rger J,Mielke T,Scheerer P,Spahn CM

Whole-exome sequencing and functional studies identify RPS29 as a novel gene mutated in multicase Diamond-Blackfan anemia families.
Blood124(1)24-32(2014 Jul)
Mirabello L,Macari ER,Jessop L,Ellis SR,Myers T,Giri N,Taylor AM,McGrath KE,Humphries JM,Ballew BJ,Yeager M,Boland JF,He J,Hicks BD,Burdett L,Alter BP,Zon L,Savage SA

Genome-wide association study identifies new susceptibility loci for posttraumatic stress disorder.
Biological psychiatry74(9)656-63(2013 Nov)
Xie P,Kranzler HR,Yang C,Zhao H,Farrer LA,Gelernter J

Structures of the human and Drosophila 80S ribosome.
Nature497(7447)80-5(2013 May)
Anger AM,Armache JP,Berninghausen O,Habeck M,Subklewe M,Wilson DN,Beckmann R

Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women.
PLoS genetics8(5)e1002695(2012)
Fox CS,Liu Y,White CC,Feitosa M,Smith AV,Heard-Costa N,Lohman K,,,,Johnson AD,Foster MC,Greenawalt DM,Griffin P,Ding J,Newman AB,Tylavsky F,Miljkovic I,Kritchevsky SB,Launer L,Garcia M,Eiriksdottir G,Carr JJ,Gudnason V,Harris TB,Cupples LA,Borecki IB

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RPS29 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

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