RPS25 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPS25
Entrez Gene ID	6230
Full Name	ribosomal protein S25
Synonyms	S25
General protein information	Preferred Names ribosomal protein S25 Names 40S ribosomal protein S25 small ribosomal subunit protein eS25
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q23.3
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S25E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008].
Disorder MIM:	180465

mRNA and Protein(s)

mRNA	Protein	Name
NM_001028.2	NP_001019.1	40S ribosomal protein S25
NM_001028.3	NP_001019.1	40S ribosomal protein S25

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	RPS25	XP_001234052.1
Danio rerio (zebrafish)	rps25	NP_957109.1
	LOC719947	XP_002801195.1
Mus musculus (house mouse)	Rps25	NP_077228.1
Drosophila melanogaster (fruit fly)	RpS25	NP_524315.2
Bos taurus (cattle)	RPS25	NP_001020486.1
Rattus norvegicus (Norway rat)	Rps25	NP_001005528.1
Caenorhabditis elegans (roundworm)	rps-25	NP_500895.1
Homo sapiens (human)	RPS25	NP_001019.1
Pan troglodytes (chimpanzee)	RPS25	NP_001238922.1
Canis lupus familiaris (dog)	RPS25	XP_536549.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004462	XP_313760.4

Gene Ontology
Bibliography

Related articles in PubMed

HBZ-mediated shift of JunD from growth suppressor to tumor promoter in leukemic cells by inhibition of ribosomal protein S25 expression.
Terol M, Gazon H, Lemasson I, Duc-Dodon M, Barbeau B, C?saire R, Mesnard JM, P?lopon?se JM Jr
Leukemia31(10)2235-2243(2017 Oct)

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP
Journal of virology88(18)10340-53(2014 Sep)

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Zanivan S, Maione F, Hein MY, Hern?ndez-Fernaud JR, Ostasiewicz P, Giraudo E, Mann M
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)

Identification of ribosomal protein S25 (RPS25)-MDM2-p53 regulatory feedback loop.
Zhang X, Wang W, Wang H, Wang MH, Xu W, Zhang R
Oncogene32(22)2782-91(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HBZ induces nuclear retention of RPS25 mRNA in the nucleus and loss of RPS25 protein expression. Inhibition of RPS25 expression stimulates Delta JunD synthesis.
Title: HBZ-mediated shift of JunD from growth suppressor to tumor promoter in leukemic cells by inhibition of ribosomal protein S25 expression.

data demonstrate that ribosomal protein S25 is a novel regulator of the MDM2-p53 circuit in ribosomal stress; results shed light on biological consequences of RP-MDM2-p53 interplay and also reveal the existence of a novel S25-p53 feedback regulatory loop
Title: Identification of ribosomal protein S25 (RPS25)-MDM2-p53 regulatory feedback loop.

The following RPS25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPS25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13935	NM_001028.3 Latest version!	Homo sapiens ribosomal protein S25 (RPS25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13935	NM_001028.2	Homo sapiens ribosomal protein S25 (RPS25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPS25 ( NM_001028.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001019.1 Homo sapiens ribosomal protein S25 (RPS25), mRNA.

Price & Availability↑

CloneID	OHu13935
Clone ID Related Accession (Same CDS sequence)	NM_001028.2 , NM_001028.3
Accession Version	NM_001028.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Homo sapiens(human)
Product	40S ribosomal protein S25
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from D28369.1 and BC004986.1. On Jul 3, 2001 this sequence version replaced NM_001028.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP261739.1, SRR1163657.23826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCGCCTA AGGACGACAA GAAGAAGAAG GACGCTGGAA AGTCGGCCAA GAAAGACAAA 
GACCCAGTGA ACAAATCCGG GGGCAAGGCC AAAAAGAAGA AGTGGTCCAA AGGCAAAGTT 
CGGGACAAGC TCAATAACTT AGTCTTGTTT GACAAAGCTA CCTATGATAA ACTCTGTAAG 
GAAGTTCCCA ACTATAAACT TATAACCCCA GCTGTGGTCT CTGAGAGACT GAAGATTCGA 
GGCTCCCTGG CCAGGGCAGC CCTTCAGGAG CTCCTTAGTA AAGGACTTAT CAAACTGGTT 
TCAAAGCACA GAGCTCAAGT AATTTACACC AGAAATACCA AGGGTGGAGA TGCTCCAGCT 
GCTGGTGAAG ATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019.1
CDS	64..441
Translation	MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIRGSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA

Target ORF information:

RefSeq Version	NM_001028.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S25 (RPS25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028.2

ORF Insert Sequence:

ATGCCGCCTA AGGACGACAA GAAGAAGAAG GACGCTGGAA AGTCGGCCAA GAAAGACAAA 
GACCCAGTGA ACAAATCCGG GGGCAAGGCC AAAAAGAAGA AGTGGTCCAA AGGCAAAGTT 
CGGGACAAGC TCAATAACTT AGTCTTGTTT GACAAAGCTA CCTATGATAA ACTCTGTAAG 
GAAGTTCCCA ACTATAAACT TATAACCCCA GCTGTGGTCT CTGAGAGACT GAAGATTCGA 
GGCTCCCTGG CCAGGGCAGC CCTTCAGGAG CTCCTTAGTA AAGGACTTAT CAAACTGGTT 
TCAAAGCACA GAGCTCAAGT AATTTACACC AGAAATACCA AGGGTGGAGA TGCTCCAGCT 
GCTGGTGAAG ATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS25 ( NM_001028.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001019.1 Homo sapiens ribosomal protein S25 (RPS25), mRNA.

Price & Availability↑

CloneID	OHu13935
Clone ID Related Accession (Same CDS sequence)	NM_001028.2 , NM_001028.3
Accession Version	NM_001028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	40S ribosomal protein S25
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from D28369.1 and BC004986.1. On Nov 23, 2018 this sequence version replaced NM_001028.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.124115.1, SRR1163657.237885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000527673.2/ ENSP00000435096.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGCCTA AGGACGACAA GAAGAAGAAG GACGCTGGAA AGTCGGCCAA GAAAGACAAA 
GACCCAGTGA ACAAATCCGG GGGCAAGGCC AAAAAGAAGA AGTGGTCCAA AGGCAAAGTT 
CGGGACAAGC TCAATAACTT AGTCTTGTTT GACAAAGCTA CCTATGATAA ACTCTGTAAG 
GAAGTTCCCA ACTATAAACT TATAACCCCA GCTGTGGTCT CTGAGAGACT GAAGATTCGA 
GGCTCCCTGG CCAGGGCAGC CCTTCAGGAG CTCCTTAGTA AAGGACTTAT CAAACTGGTT 
TCAAAGCACA GAGCTCAAGT AATTTACACC AGAAATACCA AGGGTGGAGA TGCTCCAGCT 
GCTGGTGAAG ATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019.1
CDS	60..437
Translation	MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIRGSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA

Target ORF information:

RefSeq Version	NM_001028.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S25 (RPS25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028.3

ORF Insert Sequence:

ATGCCGCCTA AGGACGACAA GAAGAAGAAG GACGCTGGAA AGTCGGCCAA GAAAGACAAA 
GACCCAGTGA ACAAATCCGG GGGCAAGGCC AAAAAGAAGA AGTGGTCCAA AGGCAAAGTT 
CGGGACAAGC TCAATAACTT AGTCTTGTTT GACAAAGCTA CCTATGATAA ACTCTGTAAG 
GAAGTTCCCA ACTATAAACT TATAACCCCA GCTGTGGTCT CTGAGAGACT GAAGATTCGA 
GGCTCCCTGG CCAGGGCAGC CCTTCAGGAG CTCCTTAGTA AAGGACTTAT CAAACTGGTT 
TCAAAGCACA GAGCTCAAGT AATTTACACC AGAAATACCA AGGGTGGAGA TGCTCCAGCT 
GCTGGTGAAG ATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HBZ-mediated shift of JunD from growth suppressor to tumor promoter in leukemic cells by inhibition of ribosomal protein S25 expression.
Leukemia31(10)2235-2243(2017 Oct)
Terol M,Gazon H,Lemasson I,Duc-Dodon M,Barbeau B,C?saire R,Mesnard JM,P?lopon?se JM Jr

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Journal of virology88(18)10340-53(2014 Sep)
Jefferson M,Donaszi-Ivanov A,Pollen S,Dalmay T,Saalbach G,Powell PP

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)
Zanivan S,Maione F,Hein MY,Hern?ndez-Fernaud JR,Ostasiewicz P,Giraudo E,Mann M

Identification of ribosomal protein S25 (RPS25)-MDM2-p53 regulatory feedback loop.
Oncogene32(22)2782-91(2013 May)
Zhang X,Wang W,Wang H,Wang MH,Xu W,Zhang R

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RPS25 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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