RPS20 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPS20
Entrez Gene ID	6224
Full Name	ribosomal protein S20
Synonyms	S20
General protein information	Preferred Names ribosomal protein S20 Names 40S ribosomal protein S20 small ribosomal subunit protein uS10
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q12.1
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2009].
Disorder MIM:	603682

mRNA and Protein(s)

mRNA	Protein	Name
NM_001146227.1	NP_001139699.1	40S ribosomal protein S20 isoform 1
NM_001023.3	NP_001014.1	40S ribosomal protein S20 isoform 2
NM_001146227.2	NP_001139699.1	40S ribosomal protein S20 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RPS20	XP_535079.2
Saccharomyces cerevisiae (baker's yeast)	RPS20	NP_011848.1
Oryza sativa (rice)	Os03g0249400	NP_001049560.1
Pan troglodytes (chimpanzee)	RPS20	XP_001154969.1
Rattus norvegicus (Norway rat)	Rps20	NP_001007604.1
Caenorhabditis elegans (roundworm)	rps-20	NP_740944.1
Kluyveromyces lactis	KLLA0F25542g	XP_456218.1
Schizosaccharomyces pombe (fission yeast)	rps20	NP_588436.1
Oryza sativa (rice)	Os06g0134000	NP_001056713.1
Xenopus tropicalis (tropical clawed frog)	rbm6	NP_001005106.1
	RPS20	XP_001085267.1
Bos taurus (cattle)	RPS20	NP_001029610.1
Mus musculus (house mouse)	Rps20	NP_080423.1
Magnaporthe oryzae (rice blast fungus)	MGG_03372	XP_003716574.1
Homo sapiens (human)	RPS20	NP_001014.1
Danio rerio (zebrafish)	rps20	NP_998369.1
Eremothecium gossypii	AGOS_ABR041C	NP_982987.1
Neurospora crassa	NCU06892	XP_959239.1
Arabidopsis thaliana (thale cress)	AT5G62300	NP_201036.1
Arabidopsis thaliana (thale cress)	AT3G47370	NP_190321.1
Gallus gallus (chicken)	RPS20	XP_428540.3
Drosophila melanogaster (fruit fly)	RpS20	NP_524421.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010591	XP_314556.3
Arabidopsis thaliana (thale cress)	AT3G45030	NP_190089.2
Oryza sativa (rice)	Os10g0170200	NP_001064232.1

Gene Ontology
Bibliography

Related articles in PubMed

ZNF598 and RACK1 Regulate Mammalian Ribosome-Associated Quality Control Function by Mediating Regulatory 40S Ribosomal Ubiquitylation.
Sundaramoorthy E, Leonard M, Mak R, Liao J, Fulzele A, Bennett EJ
Molecular cell65(4)751-760.e4(2017 Feb)

Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination.
Juszkiewicz S, Hegde RS
Molecular cell65(4)743-750.e4(2017 Feb)

Validation of Recently Proposed Colorectal Cancer Susceptibility Gene Variants in an Analysis of Families and Patients-a Systematic Review.
Broderick P, Dobbins SE, Chubb D, Kinnersley B, Dunlop MG, Tomlinson I, Houlston RS
Gastroenterology152(1)75-77.e4(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We therefore found published evidence to support the association between variants in NTHL1 and RPS20 with CRC.
Title: Validation of Recently Proposed Colorectal Cancer Susceptibility Gene Variants in an Analysis of Families and Patients-a Systematic Review.

increased expression of RPS11 and RPS20 predicts shorter patient survival. treatment-resistant GSC clones are clinically relevant cells that represent resistant tumorigenic clones from patient tumors and that their properties
Title: Ribosomal Proteins RPS11 and RPS20, Two Stress-Response Markers of Glioblastoma Stem Cells, Are Novel Predictors of Poor Prognosis in Glioblastoma Patients.

Mutations in RPS20 can predispose individuals to microsatellite-stable hereditary nonpolyposis colorectal carcinoma.
Title: Germline mutation of RPS20, encoding a ribosomal protein, causes predisposition to hereditary nonpolyposis colorectal carcinoma without DNA mismatch repair deficiency.

RPL37, RPS15 and RPS20 regulate the Mdm2-p53-MdmX network but employ different mechanisms to do so.
Title: Ribosomal proteins RPL37, RPS15 and RPS20 regulate the Mdm2-p53-MdmX network.

Observational study of gene-disease association. (HuGE Navigator)
Title: The role of height-associated loci identified in genome wide association studies in the determination of pediatric stature.

The following RPS20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPS20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09230	NM_001023.3 Latest version!	Homo sapiens ribosomal protein S20 (RPS20), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu09196	NM_001146227.2 Latest version!	Homo sapiens ribosomal protein S20 (RPS20), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu09196	NM_001146227.1	Homo sapiens ribosomal protein S20 (RPS20), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPS20 ( NM_001023.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001014.1 Homo sapiens ribosomal protein S20 (RPS20), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09230
Clone ID Related Accession (Same CDS sequence)	NM_001023.3
Accession Version	NM_001023.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	40S ribosomal protein S20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from CB138358.1, BC007507.2 and BQ064297.1. On Apr 2, 2009 this sequence version replaced NM_001023.2. Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ064297.1, SRR1660807.107735.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTTA AGGATACCGG AAAAACACCC GTGGAGCCGG AGGTGGCAAT TCACCGAATT 
CGAATCACCC TAACAAGCCG CAACGTAAAA TCCTTGGAAA AGGTGTGTGC TGACTTGATA 
AGAGGCGCAA AAGAAAAGAA TCTCAAAGTG AAAGGACCAG TTCGAATGCC TACCAAGACT 
TTGAGAATCA CTACAAGAAA AACTCCTTGT GGTGAAGGTT CTAAGACGTG GGATCGTTTC 
CAGATGAGAA TTCACAAGCG ACTCATTGAC TTGCACAGTC CTTCTGAGAT TGTTAAGCAG 
ATTACTTCCA TCAGTATTGA GCCAGGAGTT GAGGTGGAAG TCACCATTGC AGATGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014.1
CDS	199..558
Translation	MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA

Target ORF information:

RefSeq Version	NM_001023.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S20 (RPS20), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001023.3

ORF Insert Sequence:

ATGGCTTTTA AGGATACCGG AAAAACACCC GTGGAGCCGG AGGTGGCAAT TCACCGAATT 
CGAATCACCC TAACAAGCCG CAACGTAAAA TCCTTGGAAA AGGTGTGTGC TGACTTGATA 
AGAGGCGCAA AAGAAAAGAA TCTCAAAGTG AAAGGACCAG TTCGAATGCC TACCAAGACT 
TTGAGAATCA CTACAAGAAA AACTCCTTGT GGTGAAGGTT CTAAGACGTG GGATCGTTTC 
CAGATGAGAA TTCACAAGCG ACTCATTGAC TTGCACAGTC CTTCTGAGAT TGTTAAGCAG 
ATTACTTCCA TCAGTATTGA GCCAGGAGTT GAGGTGGAAG TCACCATTGC AGATGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS20 ( NM_001146227.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139699.1 Homo sapiens ribosomal protein S20 (RPS20), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09196
Clone ID Related Accession (Same CDS sequence)	NM_001146227.1 , NM_001146227.2
Accession Version	NM_001146227.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	40S ribosomal protein S20 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB138358.1, AK301342.1 and CD671362.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301342.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2155371 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTTTA AGGATACCGG AAAAACACCC GTGGAGCCGG AGGTGGCAAT TCACCGAATT 
CGAATCACCC TAACAAGCCG CAACGTAAAA TCCTTGGAAA AGGTGTGTGC TGACTTGATA 
AGAGGCGCAA AAGAAAAGAA TCTCAAAGTG AAAGGACCAG TTCGAATGCC TACCAAGACT 
TTGAGAATCA CTACAAGAAA AACTCCTTGT GGTGAAGGTT CTAAGACGTG GGATCGTTTC 
CAGATGAGAA TTCACAAGCG ACTCATTGAC TTGCACAGTC CTTCTGAGAT TGTTAAGCAG 
ATTACTTCCA TCAGTATTGA GCCAGGAGTT GAGTTGATCG AATCTACAGA TGCAGAACCC 
ATGGATACAG AGGGCCAGCA GTACACTTTG AGAAGTGTAT TCGAATCCCC GGGGACATGT 
CCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139699.1
CDS	199..627
Translation	MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVELIESTDAEPMDTEGQQYTLRSVFESPGTCPF

Target ORF information:

RefSeq Version	NM_001146227.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S20 (RPS20), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146227.1

ORF Insert Sequence:

ATGGCTTTTA AGGATACCGG AAAAACACCC GTGGAGCCGG AGGTGGCAAT TCACCGAATT 
CGAATCACCC TAACAAGCCG CAACGTAAAA TCCTTGGAAA AGGTGTGTGC TGACTTGATA 
AGAGGCGCAA AAGAAAAGAA TCTCAAAGTG AAAGGACCAG TTCGAATGCC TACCAAGACT 
TTGAGAATCA CTACAAGAAA AACTCCTTGT GGTGAAGGTT CTAAGACGTG GGATCGTTTC 
CAGATGAGAA TTCACAAGCG ACTCATTGAC TTGCACAGTC CTTCTGAGAT TGTTAAGCAG 
ATTACTTCCA TCAGTATTGA GCCAGGAGTT GAGTTGATCG AATCTACAGA TGCAGAACCC 
ATGGATACAG AGGGCCAGCA GTACACTTTG AGAAGTGTAT TCGAATCCCC GGGGACATGT 
CCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS20 ( NM_001146227.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139699.1 Homo sapiens ribosomal protein S20 (RPS20), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09196
Clone ID Related Accession (Same CDS sequence)	NM_001146227.1 , NM_001146227.2
Accession Version	NM_001146227.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	40S ribosomal protein S20 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK301342.1, CD671362.1 and AC107376.7. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001146227.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301342.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2155371 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTTTA AGGATACCGG AAAAACACCC GTGGAGCCGG AGGTGGCAAT TCACCGAATT 
CGAATCACCC TAACAAGCCG CAACGTAAAA TCCTTGGAAA AGGTGTGTGC TGACTTGATA 
AGAGGCGCAA AAGAAAAGAA TCTCAAAGTG AAAGGACCAG TTCGAATGCC TACCAAGACT 
TTGAGAATCA CTACAAGAAA AACTCCTTGT GGTGAAGGTT CTAAGACGTG GGATCGTTTC 
CAGATGAGAA TTCACAAGCG ACTCATTGAC TTGCACAGTC CTTCTGAGAT TGTTAAGCAG 
ATTACTTCCA TCAGTATTGA GCCAGGAGTT GAGTTGATCG AATCTACAGA TGCAGAACCC 
ATGGATACAG AGGGCCAGCA GTACACTTTG AGAAGTGTAT TCGAATCCCC GGGGACATGT 
CCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139699.1
CDS	124..552
Translation	MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVELIESTDAEPMDTEGQQYTLRSVFESPGTCPF

Target ORF information:

RefSeq Version	NM_001146227.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S20 (RPS20), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146227.2

ORF Insert Sequence:

ATGGCTTTTA AGGATACCGG AAAAACACCC GTGGAGCCGG AGGTGGCAAT TCACCGAATT 
CGAATCACCC TAACAAGCCG CAACGTAAAA TCCTTGGAAA AGGTGTGTGC TGACTTGATA 
AGAGGCGCAA AAGAAAAGAA TCTCAAAGTG AAAGGACCAG TTCGAATGCC TACCAAGACT 
TTGAGAATCA CTACAAGAAA AACTCCTTGT GGTGAAGGTT CTAAGACGTG GGATCGTTTC 
CAGATGAGAA TTCACAAGCG ACTCATTGAC TTGCACAGTC CTTCTGAGAT TGTTAAGCAG 
ATTACTTCCA TCAGTATTGA GCCAGGAGTT GAGTTGATCG AATCTACAGA TGCAGAACCC 
ATGGATACAG AGGGCCAGCA GTACACTTTG AGAAGTGTAT TCGAATCCCC GGGGACATGT 
CCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZNF598 and RACK1 Regulate Mammalian Ribosome-Associated Quality Control Function by Mediating Regulatory 40S Ribosomal Ubiquitylation.
Molecular cell65(4)751-760.e4(2017 Feb)
Sundaramoorthy E,Leonard M,Mak R,Liao J,Fulzele A,Bennett EJ

Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination.
Molecular cell65(4)743-750.e4(2017 Feb)
Juszkiewicz S,Hegde RS

Validation of Recently Proposed Colorectal Cancer Susceptibility Gene Variants in an Analysis of Families and Patients-a Systematic Review.
Gastroenterology152(1)75-77.e4(2017 Jan)
Broderick P,Dobbins SE,Chubb D,Kinnersley B,Dunlop MG,Tomlinson I,Houlston RS

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RPS20 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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