RPS10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPS10
Entrez Gene ID	6204
Full Name	ribosomal protein S10
Synonyms	DBA9,S10
General protein information	Preferred Names ribosomal protein S10 Names 40S ribosomal protein S10 small ribosomal subunit protein eS10
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.31
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternate splicing results in multiple transcript variants that encode the same protein. Naturally occurring read-through transcription occurs between this locus and the neighboring locus NUDT3 (nudix (nucleoside diphosphate linked moiety X)-type motif 3).[provided by RefSeq, Feb 2011].
Disorder MIM:	603632
Disorder Html:	Diamond-Blackfan anemia 9, 613308 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001204091.2	NP_001191020.1	40S ribosomal protein S10
NM_001203245.3	NP_001190174.1	40S ribosomal protein S10
NM_001203245.2	NP_001190174.1	40S ribosomal protein S10
NM_001014.4	NP_001005.1	40S ribosomal protein S10
NM_001204091.1	NP_001191020.1	40S ribosomal protein S10
NM_001014.5	NP_001005.1	40S ribosomal protein S10

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	RPS10	NP_001244226.1
Drosophila melanogaster (fruit fly)	RpS10a	NP_651576.1
Kluyveromyces lactis	KLLA0B08173g	XP_451894.1
Magnaporthe oryzae (rice blast fungus)	MGG_01113	XP_003717821.1
Neurospora crassa	NCU06743	XP_963515.2
Homo sapiens (human)	RPS10	NP_001005.1
	RPS10	XP_001116498.1
Mus musculus (house mouse)	Rps10	NP_080239.1
Saccharomyces cerevisiae (baker's yeast)	RPS10A	NP_014936.1
Canis lupus familiaris (dog)	RPS10	NP_001238974.1
Saccharomyces cerevisiae (baker's yeast)	RPS10B	NP_013957.1
Schizosaccharomyces pombe (fission yeast)	rps1001	NP_594018.1
Pan troglodytes (chimpanzee)	LOC742827	XP_003313051.1
Bos taurus (cattle)	RPS10	NP_001029888.1
Rattus norvegicus (Norway rat)	Rps10l1	XP_002729669.1
Rattus norvegicus (Norway rat)	Rps10	NP_112371.1
Eremothecium gossypii	AGOS_AFR569W	NP_986116.1
Schizosaccharomyces pombe (fission yeast)	rps1002	NP_595605.1

Gene Ontology
Bibliography

Related articles in PubMed

Efficient and Accurate Translation Initiation Directed by TISU Involves RPS3 and RPS10e Binding and Differential Eukaryotic Initiation Factor 1A Regulation.
Haimov O, Sinvani H, Martin F, Ulitsky I, Emmanuel R, Tamarkin-Ben-Harush A, Vardy A, Dikstein R
Molecular and cellular biology37(15)(2017 Aug)

ZNF598 and RACK1 Regulate Mammalian Ribosome-Associated Quality Control Function by Mediating Regulatory 40S Ribosomal Ubiquitylation.
Sundaramoorthy E, Leonard M, Mak R, Liao J, Fulzele A, Bennett EJ
Molecular cell65(4)751-760.e4(2017 Feb)

Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination.
Juszkiewicz S, Hegde RS
Molecular cell65(4)743-750.e4(2017 Feb)

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Zanivan S, Maione F, Hein MY, Hern?ndez-Fernaud JR, Ostasiewicz P, Giraudo E, Mann M
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Short 5'UTR mRNAs are enriched with TISU (translation initiator of short 5'UTR), a 12-nucleotide element directing efficient scanning-independent translation. This study demonstrate that TISU is particularly dependent on eukaryotic initiation factor 1A (eIF1A) which interacts with both RPS3 and RPS10e.
Title: Efficient and Accurate Translation Initiation Directed by TISU Involves RPS3 and RPS10e Binding and Differential Eukaryotic Initiation Factor 1A Regulation.

These results are consistent with a model in which the Nef protein by binding to two components of the 40S small ribosomal subunit, RPS10 and 18S rRNA, and to a lesser extent to tRNAs, could lead to decreased protein synthesis.
Title: The HIV-1 Nef protein interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA.

A novel mutation of ribosomal protein S10 gene in a Japanese patient with diamond-Blackfan anemia.
Title: A novel mutation of ribosomal protein S10 gene in a Japanese patient with diamond-Blackfan anemia.

Methylation of ribosomal protein S10 by protein-arginine methyltransferase 5 regulates ribosome biogenesis.
Title: Methylation of ribosomal protein S10 by protein-arginine methyltransferase 5 regulates ribosome biogenesis.

Mutations in the RPS10 and RPS26 genes in DBA patients affect the function of the proteins in rRNA processing.
Title: Ribosomal protein genes RPS10 and RPS26 are commonly mutated in Diamond-Blackfan anemia.

The following RPS10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPS10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20633	NM_001204091.2 Latest version!	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu20633	NM_001014.5 Latest version!	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu20633	NM_001203245.3 Latest version!	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu20633	NM_001203245.2	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu20633	NM_001204091.1	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu20633	NM_001014.4	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPS10 ( NM_001204091.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191020.1 Homo sapiens ribosomal protein S10 (RPS10), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20633
Clone ID Related Accession (Same CDS sequence)	NM_001204091.2 , NM_001203245.3 , NM_001203245.2 , NM_001014.4 , NM_001204091.1 , NM_001014.5
Accession Version	NM_001204091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	40S ribosomal protein S10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157372.18 and BU956627.1. On May 31, 2019 this sequence version replaced NM_001204091.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU956627.1, DRR138509.50446.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMN01820701 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191020.1
CDS	43..540
Translation	MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

Target ORF information:

RefSeq Version	NM_001204091.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204091.2

ORF Insert Sequence:

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS10 ( NM_001203245.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001190174.1 Homo sapiens ribosomal protein S10 (RPS10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20633
Clone ID Related Accession (Same CDS sequence)	NM_001204091.2 , NM_001203245.3 , NM_001203245.2 , NM_001014.4 , NM_001204091.1 , NM_001014.5
Accession Version	NM_001203245.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	40S ribosomal protein S10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157372.18, BG532491.1 and BC071946.1. On Jun 1, 2019 this sequence version replaced NM_001203245.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU944178.1, BQ072109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMN01820701 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190174.1
CDS	275..772
Translation	MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

Target ORF information:

RefSeq Version	NM_001203245.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203245.3

ORF Insert Sequence:

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS10 ( NM_001203245.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001190174.1 Homo sapiens ribosomal protein S10 (RPS10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20633
Clone ID Related Accession (Same CDS sequence)	NM_001204091.2 , NM_001203245.3 , NM_001203245.2 , NM_001014.4 , NM_001204091.1 , NM_001014.5
Accession Version	NM_001203245.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	40S ribosomal protein S10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG532491.1, AL157372.18 and BC071946.1. On Feb 11, 2011 this sequence version replaced NM_001203245.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.526223.1, SRR1163655.405473.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMN01820701 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190174.1
CDS	247..744
Translation	MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

Target ORF information:

RefSeq Version	NM_001203245.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203245.2

ORF Insert Sequence:

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS10 ( NM_001014.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005.1 Homo sapiens ribosomal protein S10 (RPS10), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20633
Clone ID Related Accession (Same CDS sequence)	NM_001204091.2 , NM_001203245.3 , NM_001203245.2 , NM_001014.4 , NM_001204091.1 , NM_001014.5
Accession Version	NM_001014.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	40S ribosomal protein S10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BG940847.1, AL157372.18 and BC001032.2. This sequence is a reference standard in the RefSeqGene project. On Feb 5, 2011 this sequence version replaced NM_001014.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.21792.1, SRR1163657.291252.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820701, SAMN03568911 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005.1
CDS	95..592
Translation	MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

Target ORF information:

RefSeq Version	NM_001014.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014.4

ORF Insert Sequence:

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS10 ( NM_001204091.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191020.1 Homo sapiens ribosomal protein S10 (RPS10), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20633
Clone ID Related Accession (Same CDS sequence)	NM_001204091.2 , NM_001203245.3 , NM_001203245.2 , NM_001014.4 , NM_001204091.1 , NM_001014.5
Accession Version	NM_001204091.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	40S ribosomal protein S10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157372.18 and BU956627.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU956627.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMN01820701 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191020.1
CDS	99..596
Translation	MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

Target ORF information:

RefSeq Version	NM_001204091.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204091.1

ORF Insert Sequence:

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS10 ( NM_001014.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005.1 Homo sapiens ribosomal protein S10 (RPS10), transcript variant 2, mRNA.

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CloneID	OHu20633
Clone ID Related Accession (Same CDS sequence)	NM_001204091.2 , NM_001203245.3 , NM_001203245.2 , NM_001014.4 , NM_001204091.1 , NM_001014.5
Accession Version	NM_001014.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	40S ribosomal protein S10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BG940847.1, AL157372.18 and BC001032.2. This sequence is a reference standard in the RefSeqGene project. On May 17, 2018 this sequence version replaced NM_001014.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU846121.1, BU856377.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000648437.1/ ENSP00000497917.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005.1
CDS	39..536
Translation	MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

Target ORF information:

RefSeq Version	NM_001014.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S10 (RPS10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014.5

ORF Insert Sequence:

ATGTTGATGC CTAAGAAGAA CCGGATTGCC ATTTATGAAC TCCTTTTTAA GGAGGGAGTC 
ATGGTGGCCA AGAAGGATGT CCACATGCCT AAGCACCCGG AGCTGGCAGA CAAGAATGTG 
CCCAACCTTC ATGTCATGAA GGCCATGCAG TCTCTCAAGT CCCGAGGCTA CGTGAAGGAA 
CAGTTTGCCT GGAGACATTT CTACTGGTAC CTTACCAATG AGGGTATCCA GTATCTCCGT 
GATTACCTTC ATCTGCCCCC GGAGATTGTG CCTGCCACCC TACGCCGTAG CCGTCCAGAG 
ACTGGCAGGC CTCGGCCTAA AGGTCTGGAG GGTGAGCGAC CTGCGAGACT CACAAGAGGG 
GAAGCTGACA GAGATACCTA CAGACGGAGT GCTGTGCCAC CTGGTGCCGA CAAGAAAGCC 
GAGGCTGGGG CTGGGTCAGC AACCGAATTC CAGTTTAGAG GCGGATTTGG TCGTGGACGT 
GGTCAGCCAC CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Efficient and Accurate Translation Initiation Directed by TISU Involves RPS3 and RPS10e Binding and Differential Eukaryotic Initiation Factor 1A Regulation.
Molecular and cellular biology37(15)(2017 Aug)
Haimov O,Sinvani H,Martin F,Ulitsky I,Emmanuel R,Tamarkin-Ben-Harush A,Vardy A,Dikstein R

ZNF598 and RACK1 Regulate Mammalian Ribosome-Associated Quality Control Function by Mediating Regulatory 40S Ribosomal Ubiquitylation.
Molecular cell65(4)751-760.e4(2017 Feb)
Sundaramoorthy E,Leonard M,Mak R,Liao J,Fulzele A,Bennett EJ

Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination.
Molecular cell65(4)743-750.e4(2017 Feb)
Juszkiewicz S,Hegde RS

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)
Zanivan S,Maione F,Hein MY,Hern?ndez-Fernaud JR,Ostasiewicz P,Giraudo E,Mann M

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RPS10 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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