RPS8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPS8
Entrez Gene ID	6202
Full Name	ribosomal protein S8
Synonyms	S8
General protein information	Preferred Names ribosomal protein S8 Names 40S ribosomal protein S8 OK/SW-cl.83 small ribosomal subunit protein eS8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.1
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal tumors and colon polyps compared to matched normal colonic mucosa has been observed. This gene is co-transcribed with the small nucleolar RNA genes U38A, U38B, U39, and U40, which are located in its fourth, fifth, first, and second introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008].
Disorder MIM:	600357

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012.1	NP_001003.1	40S ribosomal protein S8
NM_001012.2	NP_001003.1	40S ribosomal protein S8

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RpS8	NP_001247362.1
Caenorhabditis elegans (roundworm)	rps-8	NP_501167.1
Xenopus tropicalis (tropical clawed frog)	rps8	NP_001016566.1
Homo sapiens (human)	RPS8	NP_001003.1
	LOC696015	XP_002804244.1
Canis lupus familiaris (dog)	RPS8	XP_862274.2
Rattus norvegicus (Norway rat)	Rps8	NP_113894.1
Schizosaccharomyces pombe (fission yeast)	rps802	NP_593100.1
Bos taurus (cattle)	RPS8	NP_001020488.1
Danio rerio (zebrafish)	rps8b	NP_001128155.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002437	XP_312508.3
Kluyveromyces lactis	KLLA0E20461g	XP_454876.1
Eremothecium gossypii	AGOS_AGR375W	NP_987041.1
Arabidopsis thaliana (thale cress)	AT5G59240	NP_200732.2
Mus musculus (house mouse)	Rps8	NP_033124.1
Gallus gallus (chicken)	RPS8	NP_001239055.1
Saccharomyces cerevisiae (baker's yeast)	RPS8A	NP_009481.1
Schizosaccharomyces pombe (fission yeast)	rps801	NP_594519.1
Saccharomyces cerevisiae (baker's yeast)	RPS8B	NP_011028.1
Magnaporthe oryzae (rice blast fungus)	MGG_03251	XP_003716723.1
Neurospora crassa	NCU08500	XP_963429.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

CDK11p46 and RPS8 associate with each other and suppress translation in a synergistic manner.
Hao Y, Kong X, Ruan Y, Gan H, Chen H, Zhang C, Ren S, Gu J
Biochemical and biophysical research communications407(1)169-74(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RPS8, a member of the small subunit ribosome, was identified as an interacting partner of CDK11p46.
Title: CDK11p46 and RPS8 associate with each other and suppress translation in a synergistic manner.

The following RPS8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPS8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31095	NM_001012.2 Latest version!	Homo sapiens ribosomal protein S8 (RPS8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu31095	NM_001012.1	Homo sapiens ribosomal protein S8 (RPS8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPS8 ( NM_001012.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001003.1 Homo sapiens ribosomal protein S8 (RPS8), mRNA.

Price & Availability↑

CloneID	OHu31095
Clone ID Related Accession (Same CDS sequence)	NM_001012.1 , NM_001012.2
Accession Version	NM_001012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	40S ribosomal protein S8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from X67247.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.321356.1, SRR1163657.2707.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGCATCT CTCGGGACAA CTGGCACAAG CGCCGCAAAA CCGGGGGCAA GAGAAAGCCC 
TACCACAAGA AGCGGAAGTA TGAGTTGGGG CGCCCAGCTG CCAACACCAA GATTGGCCCC 
CGCCGCATCC ACACAGTCCG TGTGCGGGGA GGTAACAAGA AATACCGTGC CCTGAGGTTG 
GACGTGGGGA ATTTCTCCTG GGGCTCAGAG TGTTGTACTC GTAAAACAAG GATCATCGAT 
GTTGTCTACA ATGCATCTAA TAACGAGCTG GTTCGTACCA AGACCCTGGT GAAGAATTGC 
ATCGTGCTCA TCGACAGCAC ACCGTACCGA CAGTGGTACG AGTCCCACTA TGCGCTGCCC 
CTGGGCCGCA AGAAGGGAGC CAAGCTGACT CCTGAGGAAG AAGAGATTTT AAACAAAAAA 
CGATCTAAAA AAATTCAGAA GAAATATGAT GAAAGGAAAA AGAATGCCAA AATCAGCAGT 
CTCCTGGAGG AGCAGTTCCA GCAGGGCAAG CTTCTTGCGT GCATCGCTTC AAGGCCGGGA 
CAGTGTGGCC GAGCAGATGG CTATGTGCTA GAGGGCAAAG AGTTGGAGTT CTATCTTAGG 
AAAATCAAGG CCCGCAAAGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003.1
CDS	24..650
Translation	MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGK

Target ORF information:

RefSeq Version	NM_001012.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S8 (RPS8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012.1

ORF Insert Sequence:

ATGGGCATCT CTCGGGACAA CTGGCACAAG CGCCGCAAAA CCGGGGGCAA GAGAAAGCCC 
TACCACAAGA AGCGGAAGTA TGAGTTGGGG CGCCCAGCTG CCAACACCAA GATTGGCCCC 
CGCCGCATCC ACACAGTCCG TGTGCGGGGA GGTAACAAGA AATACCGTGC CCTGAGGTTG 
GACGTGGGGA ATTTCTCCTG GGGCTCAGAG TGTTGTACTC GTAAAACAAG GATCATCGAT 
GTTGTCTACA ATGCATCTAA TAACGAGCTG GTTCGTACCA AGACCCTGGT GAAGAATTGC 
ATCGTGCTCA TCGACAGCAC ACCGTACCGA CAGTGGTACG AGTCCCACTA TGCGCTGCCC 
CTGGGCCGCA AGAAGGGAGC CAAGCTGACT CCTGAGGAAG AAGAGATTTT AAACAAAAAA 
CGATCTAAAA AAATTCAGAA GAAATATGAT GAAAGGAAAA AGAATGCCAA AATCAGCAGT 
CTCCTGGAGG AGCAGTTCCA GCAGGGCAAG CTTCTTGCGT GCATCGCTTC AAGGCCGGGA 
CAGTGTGGCC GAGCAGATGG CTATGTGCTA GAGGGCAAAG AGTTGGAGTT CTATCTTAGG 
AAAATCAAGG CCCGCAAAGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS8 ( NM_001012.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001003.1 Homo sapiens ribosomal protein S8 (RPS8), mRNA.

Price & Availability↑

CloneID	OHu31095
Clone ID Related Accession (Same CDS sequence)	NM_001012.1 , NM_001012.2
Accession Version	NM_001012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	40S ribosomal protein S8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AL592166.16. On Nov 26, 2018 this sequence version replaced NM_001012.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.316090.1, SRR5189658.4207.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000396651.8/ ENSP00000379888.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCATCT CTCGGGACAA CTGGCACAAG CGCCGCAAAA CCGGGGGCAA GAGAAAGCCC 
TACCACAAGA AGCGGAAGTA TGAGTTGGGG CGCCCAGCTG CCAACACCAA GATTGGCCCC 
CGCCGCATCC ACACAGTCCG TGTGCGGGGA GGTAACAAGA AATACCGTGC CCTGAGGTTG 
GACGTGGGGA ATTTCTCCTG GGGCTCAGAG TGTTGTACTC GTAAAACAAG GATCATCGAT 
GTTGTCTACA ATGCATCTAA TAACGAGCTG GTTCGTACCA AGACCCTGGT GAAGAATTGC 
ATCGTGCTCA TCGACAGCAC ACCGTACCGA CAGTGGTACG AGTCCCACTA TGCGCTGCCC 
CTGGGCCGCA AGAAGGGAGC CAAGCTGACT CCTGAGGAAG AAGAGATTTT AAACAAAAAA 
CGATCTAAAA AAATTCAGAA GAAATATGAT GAAAGGAAAA AGAATGCCAA AATCAGCAGT 
CTCCTGGAGG AGCAGTTCCA GCAGGGCAAG CTTCTTGCGT GCATCGCTTC AAGGCCGGGA 
CAGTGTGGCC GAGCAGATGG CTATGTGCTA GAGGGCAAAG AGTTGGAGTT CTATCTTAGG 
AAAATCAAGG CCCGCAAAGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003.1
CDS	58..684
Translation	MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGK

Target ORF information:

RefSeq Version	NM_001012.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S8 (RPS8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012.2

ORF Insert Sequence:

ATGGGCATCT CTCGGGACAA CTGGCACAAG CGCCGCAAAA CCGGGGGCAA GAGAAAGCCC 
TACCACAAGA AGCGGAAGTA TGAGTTGGGG CGCCCAGCTG CCAACACCAA GATTGGCCCC 
CGCCGCATCC ACACAGTCCG TGTGCGGGGA GGTAACAAGA AATACCGTGC CCTGAGGTTG 
GACGTGGGGA ATTTCTCCTG GGGCTCAGAG TGTTGTACTC GTAAAACAAG GATCATCGAT 
GTTGTCTACA ATGCATCTAA TAACGAGCTG GTTCGTACCA AGACCCTGGT GAAGAATTGC 
ATCGTGCTCA TCGACAGCAC ACCGTACCGA CAGTGGTACG AGTCCCACTA TGCGCTGCCC 
CTGGGCCGCA AGAAGGGAGC CAAGCTGACT CCTGAGGAAG AAGAGATTTT AAACAAAAAA 
CGATCTAAAA AAATTCAGAA GAAATATGAT GAAAGGAAAA AGAATGCCAA AATCAGCAGT 
CTCCTGGAGG AGCAGTTCCA GCAGGGCAAG CTTCTTGCGT GCATCGCTTC AAGGCCGGGA 
CAGTGTGGCC GAGCAGATGG CTATGTGCTA GAGGGCAAAG AGTTGGAGTT CTATCTTAGG 
AAAATCAAGG CCCGCAAAGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

CDK11p46 and RPS8 associate with each other and suppress translation in a synergistic manner.
Biochemical and biophysical research communications407(1)169-74(2011 Apr)
Hao Y,Kong X,Ruan Y,Gan H,Chen H,Zhang C,Ren S,Gu J

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RPS8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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