RPS6KA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPS6KA1
Entrez Gene ID	6195
Full Name	ribosomal protein S6 kinase A1
Synonyms	HU-1,MAPKAPK1A,RSK,RSK1,p90Rsk
General protein information	Preferred Names ribosomal protein S6 kinase A1 Names ribosomal protein S6 kinase alpha-1 90 kDa ribosomal protein S6 kinase 1 MAP kinase-activated protein kinase 1a MAPK-activated protein kinase 1a MAPKAP kinase 1a MAPKAPK-1a RSK-1 S6K-alpha 1 dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1) p90-RSK 1 p90RSK1 p90S6K ribosomal S6 kinase 1 ribosomal protein S6 kinase, 90kDa, polypeptide 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.11
Summary	This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:	601684

mRNA and Protein(s)

mRNA	Protein	Name
XM_024448871.1	XP_024304639.1	ribosomal protein S6 kinase alpha-1 isoform X1
NM_001330441.2	NP_001317370.1	ribosomal protein S6 kinase alpha-1 isoform c
NM_002953.3	NP_002944.2	ribosomal protein S6 kinase alpha-1 isoform a
NM_001006665.1	NP_001006666.1	ribosomal protein S6 kinase alpha-1 isoform b
NM_002953.4	NP_002944.2	ribosomal protein S6 kinase alpha-1 isoform a
NM_001330441.1	NP_001317370.1	ribosomal protein S6 kinase alpha-1 isoform c
NM_001006665.2	NP_001006666.1	ribosomal protein S6 kinase alpha-1 isoform b

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RPS6KA1	XP_003307951.1
Canis lupus familiaris (dog)	RPS6KA1	XP_544479.2
Caenorhabditis elegans (roundworm)	rskn-1	NP_001021602.1
Homo sapiens (human)	RPS6KA1	NP_002944.2
	LOC714794	XP_002808324.1
Danio rerio (zebrafish)	rps6ka3a	NP_997951.1
Xenopus tropicalis (tropical clawed frog)	rps6ka1	NP_001116931.1
Rattus norvegicus (Norway rat)	Rps6ka1	NP_112369.1
Gallus gallus (chicken)	RPS6KA1	NP_001103241.1
Bos taurus (cattle)	RPS6KA1	NP_001077191.1
Mus musculus (house mouse)	Rps6ka1	NP_033123.2

Gene Ontology
Bibliography

Related articles in PubMed

L1 coupling to ankyrin and the spectrin-actin cytoskeleton modulates ethanol inhibition of L1 adhesion and ethanol teratogenesis.
Dou X, Menkari C, Mitsuyama R, Foroud T, Wetherill L, Hammond P, Suttie M, Chen X, Chen SY, Charness ME,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that UBR5 down-regulates levels of TRAF3, a key component of Toll-like receptor signaling, via the miRNA pathway; p90RSK is an upstream regulator of UBR5; p90RSK phosphorylates UBR5 as required for translational repression of TRAF3 mRNA. (UBR5 = ubiquitin protein ligase E3 component n-recognin 5 protein; TRAF3 = TNF receptor-associated factor 3; p90RSK = 90 kDa ribosomal protein S6 kinase)
Title: The p90 ribosomal S6 kinase-UBR5 pathway controls Toll-like receptor signaling via miRNA-induced translational inhibition of tumor necrosis factor receptor-associated factor 3.

VEGF-A driven Mdm2 phosphorylation on Ser166 is dependent on the ERK1/2/p90RSK signaling pathway in primary human endothelial cells.
Title: The Vascular Endothelial Growth Factor-A phosphorylates Murine Double Minute-2 on its Serine 166 via the Extracellular Signal-Regulated Kinase 1/2 and p90 Ribosomal S6 Kinase in primary human endothelial cells.

Data indicate that BTG2, MAP3K11, RPS6KA1 and PRDM1 as putative targets of microRNA miR-125b.
Title: Identification of miR-125b targets involved in acute promyelocytic leukemia cell proliferation.

The p90RSK has an essential role in promoting tumor growth and proliferation in non-small cell lung cancer (NSCLC). BID may serve as an alternative cancer treatment in NSCLC.
Title: Inhibition of p90 ribosomal S6 kinase attenuates cell migration and proliferation of the human lung adenocarcinoma through phospho-GSK-3? and osteopontin.

RSK1 and 3 but not RSK2 are down-regulated in breast tumour and are associated with disease progression. RSK may be a key component in the progression and metastasis of breast cancer.
Title: The Clinical Implications of RSK1-3 in Human Breast Cancer.

The following RPS6KA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPS6KA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03774	NM_001006665.1 Latest version!	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu04024	NM_002953.3 Latest version!	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu102158	NM_001330441.1 Latest version!	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu107202	NM_002953.4 Latest version!	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu108186	NM_001006665.2 Latest version!	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu33681	NM_001330441.2 Latest version!	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu33682	XM_024448871.1 Latest version!	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPS6KA1 ( NM_001006665.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001006666.1 Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03774
Clone ID Related Accession (Same CDS sequence)	NM_001006665.1
Accession Version	NM_001006665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-24
Organism	Homo sapiens(human)
Product	ribosomal protein S6 kinase alpha-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092955.1, BC014966.1 and L07597.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' end of the coding region compared to variant 1. These differences cause translation initiation at an in-frame AUG of an alternate exon and an isoform (b) with a distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.216612.1, SRR1803611.115812.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGG ATCCCAAGCC GCCCCGTCTG CGGCTCTGGG CCCTGATCCC CTGGCTTCCC 
AGGAAGCAGC GGCCCAGGAT CAGCCAGACC TCTCTGCCTG TCCCTGGCCC TGGCTCTGGC 
CCCCAGCGGG ACTCGGATGA GGGCGTCCTC AAGGAGATCT CCATCACGCA CCACGTCAAG 
GCTGGCTCTG AGAAGGCTGA TCCATCCCAT TTCGAGCTCC TCAAGGTTCT GGGCCAGGGA 
TCCTTTGGCA AAGTCTTCCT GGTGCGGAAA GTCACCCGGC CTGACAGTGG GCACCTGTAT 
GCTATGAAGG TGCTGAAGAA GGCAACGCTG AAAGTACGTG ACCGCGTCCG GACCAAGATG 
GAGAGAGACA TCCTGGCTGA TGTAAATCAC CCATTCGTGG TGAAGCTGCA CTATGCCTTC 
CAGACCGAGG GCAAGCTCTA TCTCATTCTG GACTTCCTGC GTGGTGGGGA CCTCTTCACC 
CGGCTCTCAA AAGAGGTGAT GTTCACGGAG GAGGATGTGA AGTTTTACCT GGCCGAGCTG 
GCTCTGGGCC TGGATCACCT GCACAGCCTG GGTATCATTT ACAGAGACCT CAAGCCTGAG 
AACATCCTTC TGGATGAGGA GGGCCACATC AAACTCACTG ACTTTGGCCT GAGCAAAGAG 
GCCATTGACC ACGAGAAGAA GGCCTATTCT TTCTGCGGGA CAGTGGAGTA CATGGCCCCT 
GAGGTCGTCA ACCGCCAGGG CCACTCCCAT AGTGCGGACT GGTGGTCCTA TGGGGTGTTG 
ATGTTTGAGA TGCTGACGGG CTCCCTGCCC TTCCAGGGGA AGGACCGGAA GGAGACCATG 
ACACTGATTC TGAAGGCGAA GCTAGGCATG CCCCAGTTTC TGAGCACTGA AGCCCAGAGC 
CTCTTGCGGG CCCTGTTCAA GCGGAATCCT GCCAACCGGC TCGGCTCCGG CCCTGATGGG 
GCAGAGGAAA TCAAGCGGCA TGTCTTCTAC TCCACCATTG ACTGGAATAA GCTATACCGT 
CGTGAGATCA AGCCACCCTT CAAGCCAGCA GTGGCTCAGC CTGATGACAC CTTCTACTTT 
GACACCGAGT TCACGTCCCG CACACCCAAG GATTCCCCAG GCATCCCCCC CAGCGCTGGG 
GCCCATCAGC TGTTCCGGGG CTTCAGCTTC GTGGCCACCG GCCTGATGGA AGACGACGGC 
AAGCCTCGTG CCCCGCAGGC ACCCCTGCAC TCGGTGGTAC AGCAACTCCA TGGGAAGAAC 
CTGGTTTTTA GTGACGGCTA CGTGGTAAAG GAGACAATTG GTGTGGGCTC CTACTCTGAG 
TGCAAGCGCT GTGTCCACAA GGCCACCAAC ATGGAGTATG CTGTCAAGGT CATTGATAAG 
AGCAAGCGGG ATCCTTCAGA AGAGATTGAG ATTCTTCTGC GGTATGGCCA GCACCCCAAC 
ATCATCACTC TGAAAGATGT GTATGATGAT GGCAAACACG TGTACCTGGT GACAGAGCTG 
ATGCGGGGTG GGGAGCTGCT GGACAAGATC CTGCGGCAGA AGTTCTTCTC AGAGCGGGAG 
GCCAGCTTTG TCCTGCACAC CATTGGCAAA ACTGTGGAGT ATCTGCACTC ACAGGGGGTT 
GTGCACAGGG ACCTGAAGCC CAGCAACATC CTGTATGTGG ACGAGTCCGG GAATCCCGAG 
TGCCTGCGCA TCTGTGACTT TGGTTTTGCC AAACAGCTGC GGGCTGAGAA TGGGCTCCTC 
ATGACACCTT GCTACACAGC CAACTTTGTG GCGCCTGAGG TGCTGAAGCG CCAGGGCTAC 
GATGAAGGCT GCGACATCTG GAGCCTGGGC ATTCTGCTGT ACACCATGCT GGCAGGATAT 
ACTCCATTTG CCAACGGTCC CAGTGACACA CCAGAGGAAA TCCTAACCCG GATCGGCAGT 
GGGAAGTTTA CCCTCAGTGG GGGAAATTGG AACACAGTTT CAGAGACAGC CAAGGACCTG 
GTGTCCAAGA TGCTACACGT GGATCCCCAC CAGCGCCTCA CAGCTAAGCA GGTTCTGCAG 
CATCCATGGG TCACCCAGAA AGACAAGCTT CCCCAAAGCC AGCTGTCCCA CCAGGACCTA 
CAGCTTGTGA AGGGAGCCAT GGCTGCCACG TACTCCGCAC TCAACAGCTC CAAGCCCACC 
CCCCAGCTGA AGCCCATCGA GTCATCCATC CTGGCCCAGC GGCGAGTGAG GAAGTTGCCA 
TCCACCACCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006666.1
CDS	50..2284
Translation	MEQDPKPPRLRLWALIPWLPRKQRPRISQTSLPVPGPGSGPQRDSDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL

Target ORF information:

RefSeq Version	NM_001006665.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006665.1

ORF Insert Sequence:

ATGGAGCAGG ATCCCAAGCC GCCCCGTCTG CGGCTCTGGG CCCTGATCCC CTGGCTTCCC 
AGGAAGCAGC GGCCCAGGAT CAGCCAGACC TCTCTGCCTG TCCCTGGCCC TGGCTCTGGC 
CCCCAGCGGG ACTCGGATGA GGGCGTCCTC AAGGAGATCT CCATCACGCA CCACGTCAAG 
GCTGGCTCTG AGAAGGCTGA TCCATCCCAT TTCGAGCTCC TCAAGGTTCT GGGCCAGGGA 
TCCTTTGGCA AAGTCTTCCT GGTGCGGAAA GTCACCCGGC CTGACAGTGG GCACCTGTAT 
GCTATGAAGG TGCTGAAGAA GGCAACGCTG AAAGTACGTG ACCGCGTCCG GACCAAGATG 
GAGAGAGACA TCCTGGCTGA TGTAAATCAC CCATTCGTGG TGAAGCTGCA CTATGCCTTC 
CAGACCGAGG GCAAGCTCTA TCTCATTCTG GACTTCCTGC GTGGTGGGGA CCTCTTCACC 
CGGCTCTCAA AAGAGGTGAT GTTCACGGAG GAGGATGTGA AGTTTTACCT GGCCGAGCTG 
GCTCTGGGCC TGGATCACCT GCACAGCCTG GGTATCATTT ACAGAGACCT CAAGCCTGAG 
AACATCCTTC TGGATGAGGA GGGCCACATC AAACTCACTG ACTTTGGCCT GAGCAAAGAG 
GCCATTGACC ACGAGAAGAA GGCCTATTCT TTCTGCGGGA CAGTGGAGTA CATGGCCCCT 
GAGGTCGTCA ACCGCCAGGG CCACTCCCAT AGTGCGGACT GGTGGTCCTA TGGGGTGTTG 
ATGTTTGAGA TGCTGACGGG CTCCCTGCCC TTCCAGGGGA AGGACCGGAA GGAGACCATG 
ACACTGATTC TGAAGGCGAA GCTAGGCATG CCCCAGTTTC TGAGCACTGA AGCCCAGAGC 
CTCTTGCGGG CCCTGTTCAA GCGGAATCCT GCCAACCGGC TCGGCTCCGG CCCTGATGGG 
GCAGAGGAAA TCAAGCGGCA TGTCTTCTAC TCCACCATTG ACTGGAATAA GCTATACCGT 
CGTGAGATCA AGCCACCCTT CAAGCCAGCA GTGGCTCAGC CTGATGACAC CTTCTACTTT 
GACACCGAGT TCACGTCCCG CACACCCAAG GATTCCCCAG GCATCCCCCC CAGCGCTGGG 
GCCCATCAGC TGTTCCGGGG CTTCAGCTTC GTGGCCACCG GCCTGATGGA AGACGACGGC 
AAGCCTCGTG CCCCGCAGGC ACCCCTGCAC TCGGTGGTAC AGCAACTCCA TGGGAAGAAC 
CTGGTTTTTA GTGACGGCTA CGTGGTAAAG GAGACAATTG GTGTGGGCTC CTACTCTGAG 
TGCAAGCGCT GTGTCCACAA GGCCACCAAC ATGGAGTATG CTGTCAAGGT CATTGATAAG 
AGCAAGCGGG ATCCTTCAGA AGAGATTGAG ATTCTTCTGC GGTATGGCCA GCACCCCAAC 
ATCATCACTC TGAAAGATGT GTATGATGAT GGCAAACACG TGTACCTGGT GACAGAGCTG 
ATGCGGGGTG GGGAGCTGCT GGACAAGATC CTGCGGCAGA AGTTCTTCTC AGAGCGGGAG 
GCCAGCTTTG TCCTGCACAC CATTGGCAAA ACTGTGGAGT ATCTGCACTC ACAGGGGGTT 
GTGCACAGGG ACCTGAAGCC CAGCAACATC CTGTATGTGG ACGAGTCCGG GAATCCCGAG 
TGCCTGCGCA TCTGTGACTT TGGTTTTGCC AAACAGCTGC GGGCTGAGAA TGGGCTCCTC 
ATGACACCTT GCTACACAGC CAACTTTGTG GCGCCTGAGG TGCTGAAGCG CCAGGGCTAC 
GATGAAGGCT GCGACATCTG GAGCCTGGGC ATTCTGCTGT ACACCATGCT GGCAGGATAT 
ACTCCATTTG CCAACGGTCC CAGTGACACA CCAGAGGAAA TCCTAACCCG GATCGGCAGT 
GGGAAGTTTA CCCTCAGTGG GGGAAATTGG AACACAGTTT CAGAGACAGC CAAGGACCTG 
GTGTCCAAGA TGCTACACGT GGATCCCCAC CAGCGCCTCA CAGCTAAGCA GGTTCTGCAG 
CATCCATGGG TCACCCAGAA AGACAAGCTT CCCCAAAGCC AGCTGTCCCA CCAGGACCTA 
CAGCTTGTGA AGGGAGCCAT GGCTGCCACG TACTCCGCAC TCAACAGCTC CAAGCCCACC 
CCCCAGCTGA AGCCCATCGA GTCATCCATC CTGGCCCAGC GGCGAGTGAG GAAGTTGCCA 
TCCACCACCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS6KA1 ( NM_002953.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002944.2 Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04024
Clone ID Related Accession (Same CDS sequence)	NM_002953.3
Accession Version	NM_002953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	ribosomal protein S6 kinase alpha-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF982517.1, BC014966.1, BM836865.1, L07597.1 and AK092955.1. On Dec 3, 2004 this sequence version replaced NM_002953.2. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014966.1, SRR1803615.117813.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCTCG CCCAGCTCAA GGAGCCCTGG CCGCTCATGG AGCTAGTGCC TCTGGACCCG 
GAGAATGGAC AGACCTCAGG GGAAGAAGCT GGACTTCAGC CGTCCAAGGA TGAGGGCGTC 
CTCAAGGAGA TCTCCATCAC GCACCACGTC AAGGCTGGCT CTGAGAAGGC TGATCCATCC 
CATTTCGAGC TCCTCAAGGT TCTGGGCCAG GGATCCTTTG GCAAAGTCTT CCTGGTGCGG 
AAAGTCACCC GGCCTGACAG TGGGCACCTG TATGCTATGA AGGTGCTGAA GAAGGCAACG 
CTGAAAGTAC GTGACCGCGT CCGGACCAAG ATGGAGAGAG ACATCCTGGC TGATGTAAAT 
CACCCATTCG TGGTGAAGCT GCACTATGCC TTCCAGACCG AGGGCAAGCT CTATCTCATT 
CTGGACTTCC TGCGTGGTGG GGACCTCTTC ACCCGGCTCT CAAAAGAGGT GATGTTCACG 
GAGGAGGATG TGAAGTTTTA CCTGGCCGAG CTGGCTCTGG GCCTGGATCA CCTGCACAGC 
CTGGGTATCA TTTACAGAGA CCTCAAGCCT GAGAACATCC TTCTGGATGA GGAGGGCCAC 
ATCAAACTCA CTGACTTTGG CCTGAGCAAA GAGGCCATTG ACCACGAGAA GAAGGCCTAT 
TCTTTCTGCG GGACAGTGGA GTACATGGCC CCTGAGGTCG TCAACCGCCA GGGCCACTCC 
CATAGTGCGG ACTGGTGGTC CTATGGGGTG TTGATGTTTG AGATGCTGAC GGGCTCCCTG 
CCCTTCCAGG GGAAGGACCG GAAGGAGACC ATGACACTGA TTCTGAAGGC GAAGCTAGGC 
ATGCCCCAGT TTCTGAGCAC TGAAGCCCAG AGCCTCTTGC GGGCCCTGTT CAAGCGGAAT 
CCTGCCAACC GGCTCGGCTC CGGCCCTGAT GGGGCAGAGG AAATCAAGCG GCATGTCTTC 
TACTCCACCA TTGACTGGAA TAAGCTATAC CGTCGTGAGA TCAAGCCACC CTTCAAGCCA 
GCAGTGGCTC AGCCTGATGA CACCTTCTAC TTTGACACCG AGTTCACGTC CCGCACACCC 
AAGGATTCCC CAGGCATCCC CCCCAGCGCT GGGGCCCATC AGCTGTTCCG GGGCTTCAGC 
TTCGTGGCCA CCGGCCTGAT GGAAGACGAC GGCAAGCCTC GTGCCCCGCA GGCACCCCTG 
CACTCGGTGG TACAGCAACT CCATGGGAAG AACCTGGTTT TTAGTGACGG CTACGTGGTA 
AAGGAGACAA TTGGTGTGGG CTCCTACTCT GAGTGCAAGC GCTGTGTCCA CAAGGCCACC 
AACATGGAGT ATGCTGTCAA GGTCATTGAT AAGAGCAAGC GGGATCCTTC AGAAGAGATT 
GAGATTCTTC TGCGGTATGG CCAGCACCCC AACATCATCA CTCTGAAAGA TGTGTATGAT 
GATGGCAAAC ACGTGTACCT GGTGACAGAG CTGATGCGGG GTGGGGAGCT GCTGGACAAG 
ATCCTGCGGC AGAAGTTCTT CTCAGAGCGG GAGGCCAGCT TTGTCCTGCA CACCATTGGC 
AAAACTGTGG AGTATCTGCA CTCACAGGGG GTTGTGCACA GGGACCTGAA GCCCAGCAAC 
ATCCTGTATG TGGACGAGTC CGGGAATCCC GAGTGCCTGC GCATCTGTGA CTTTGGTTTT 
GCCAAACAGC TGCGGGCTGA GAATGGGCTC CTCATGACAC CTTGCTACAC AGCCAACTTT 
GTGGCGCCTG AGGTGCTGAA GCGCCAGGGC TACGATGAAG GCTGCGACAT CTGGAGCCTG 
GGCATTCTGC TGTACACCAT GCTGGCAGGA TATACTCCAT TTGCCAACGG TCCCAGTGAC 
ACACCAGAGG AAATCCTAAC CCGGATCGGC AGTGGGAAGT TTACCCTCAG TGGGGGAAAT 
TGGAACACAG TTTCAGAGAC AGCCAAGGAC CTGGTGTCCA AGATGCTACA CGTGGATCCC 
CACCAGCGCC TCACAGCTAA GCAGGTTCTG CAGCATCCAT GGGTCACCCA GAAAGACAAG 
CTTCCCCAAA GCCAGCTGTC CCACCAGGAC CTACAGCTTG TGAAGGGAGC CATGGCTGCC 
ACGTACTCCG CACTCAACAG CTCCAAGCCC ACCCCCCAGC TGAAGCCCAT CGAGTCATCC 
ATCCTGGCCC AGCGGCGAGT GAGGAAGTTG CCATCCACCA CCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002944.2
CDS	164..2371
Translation	MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL

Target ORF information:

RefSeq Version	NM_002953.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002953.3

ORF Insert Sequence:

ATGCCGCTCG CCCAGCTCAA GGAGCCCTGG CCGCTCATGG AGCTAGTGCC TCTGGACCCG 
GAGAATGGAC AGACCTCAGG GGAAGAAGCT GGACTTCAGC CGTCCAAGGA TGAGGGCGTC 
CTCAAGGAGA TCTCCATCAC GCACCACGTC AAGGCTGGCT CTGAGAAGGC TGATCCATCC 
CATTTCGAGC TCCTCAAGGT TCTGGGCCAG GGATCCTTTG GCAAAGTCTT CCTGGTGCGG 
AAAGTCACCC GGCCTGACAG TGGGCACCTG TATGCTATGA AGGTGCTGAA GAAGGCAACG 
CTGAAAGTAC GTGACCGCGT CCGGACCAAG ATGGAGAGAG ACATCCTGGC TGATGTAAAT 
CACCCATTCG TGGTGAAGCT GCACTATGCC TTCCAGACCG AGGGCAAGCT CTATCTCATT 
CTGGACTTCC TGCGTGGTGG GGACCTCTTC ACCCGGCTCT CAAAAGAGGT GATGTTCACG 
GAGGAGGATG TGAAGTTTTA CCTGGCCGAG CTGGCTCTGG GCCTGGATCA CCTGCACAGC 
CTGGGTATCA TTTACAGAGA CCTCAAGCCT GAGAACATCC TTCTGGATGA GGAGGGCCAC 
ATCAAACTCA CTGACTTTGG CCTGAGCAAA GAGGCCATTG ACCACGAGAA GAAGGCCTAT 
TCTTTCTGCG GGACAGTGGA GTACATGGCC CCTGAGGTCG TCAACCGCCA GGGCCACTCC 
CATAGTGCGG ACTGGTGGTC CTATGGGGTG TTGATGTTTG AGATGCTGAC GGGCTCCCTG 
CCCTTCCAGG GGAAGGACCG GAAGGAGACC ATGACACTGA TTCTGAAGGC GAAGCTAGGC 
ATGCCCCAGT TTCTGAGCAC TGAAGCCCAG AGCCTCTTGC GGGCCCTGTT CAAGCGGAAT 
CCTGCCAACC GGCTCGGCTC CGGCCCTGAT GGGGCAGAGG AAATCAAGCG GCATGTCTTC 
TACTCCACCA TTGACTGGAA TAAGCTATAC CGTCGTGAGA TCAAGCCACC CTTCAAGCCA 
GCAGTGGCTC AGCCTGATGA CACCTTCTAC TTTGACACCG AGTTCACGTC CCGCACACCC 
AAGGATTCCC CAGGCATCCC CCCCAGCGCT GGGGCCCATC AGCTGTTCCG GGGCTTCAGC 
TTCGTGGCCA CCGGCCTGAT GGAAGACGAC GGCAAGCCTC GTGCCCCGCA GGCACCCCTG 
CACTCGGTGG TACAGCAACT CCATGGGAAG AACCTGGTTT TTAGTGACGG CTACGTGGTA 
AAGGAGACAA TTGGTGTGGG CTCCTACTCT GAGTGCAAGC GCTGTGTCCA CAAGGCCACC 
AACATGGAGT ATGCTGTCAA GGTCATTGAT AAGAGCAAGC GGGATCCTTC AGAAGAGATT 
GAGATTCTTC TGCGGTATGG CCAGCACCCC AACATCATCA CTCTGAAAGA TGTGTATGAT 
GATGGCAAAC ACGTGTACCT GGTGACAGAG CTGATGCGGG GTGGGGAGCT GCTGGACAAG 
ATCCTGCGGC AGAAGTTCTT CTCAGAGCGG GAGGCCAGCT TTGTCCTGCA CACCATTGGC 
AAAACTGTGG AGTATCTGCA CTCACAGGGG GTTGTGCACA GGGACCTGAA GCCCAGCAAC 
ATCCTGTATG TGGACGAGTC CGGGAATCCC GAGTGCCTGC GCATCTGTGA CTTTGGTTTT 
GCCAAACAGC TGCGGGCTGA GAATGGGCTC CTCATGACAC CTTGCTACAC AGCCAACTTT 
GTGGCGCCTG AGGTGCTGAA GCGCCAGGGC TACGATGAAG GCTGCGACAT CTGGAGCCTG 
GGCATTCTGC TGTACACCAT GCTGGCAGGA TATACTCCAT TTGCCAACGG TCCCAGTGAC 
ACACCAGAGG AAATCCTAAC CCGGATCGGC AGTGGGAAGT TTACCCTCAG TGGGGGAAAT 
TGGAACACAG TTTCAGAGAC AGCCAAGGAC CTGGTGTCCA AGATGCTACA CGTGGATCCC 
CACCAGCGCC TCACAGCTAA GCAGGTTCTG CAGCATCCAT GGGTCACCCA GAAAGACAAG 
CTTCCCCAAA GCCAGCTGTC CCACCAGGAC CTACAGCTTG TGAAGGGAGC CATGGCTGCC 
ACGTACTCCG CACTCAACAG CTCCAAGCCC ACCCCCCAGC TGAAGCCCAT CGAGTCATCC 
ATCCTGGCCC AGCGGCGAGT GAGGAAGTTG CCATCCACCA CCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS6KA1 ( NM_001330441.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317370.1 Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 3, mRNA.

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CloneID	OHu102158
Clone ID Related Accession (Same CDS sequence)	NM_001330441.1
Accession Version	NM_001330441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-24
Organism	Homo sapiens(human)
Product	ribosomal protein S6 kinase alpha-1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627313.16 and DC428094.1. On Aug 29, 2016 this sequence version replaced XM_005245966.4. Transcript Variant: This variant (3) differs in the 5' UTR and 5' end of the coding region compared to variant 1. These differences cause translation initiation at an in-frame AUG of an alternate exon and an isoform (c) with a distinct N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.187271.1, SRR1803611.278817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGACCC CAGCAGATTT CCCCAGGGTT GAGAGAGACT TGGTTCCCTG TCCCAGAAAG 
GATGAGGGCG TCCTCAAGGA GATCTCCATC ACGCACCACG TCAAGGCTGG CTCTGAGAAG 
GCTGATCCAT CCCATTTCGA GCTCCTCAAG GTTCTGGGCC AGGGATCCTT TGGCAAAGTC 
TTCCTGGTGC GGAAAGTCAC CCGGCCTGAC AGTGGGCACC TGTATGCTAT GAAGGTGCTG 
AAGAAGGCAA CGCTGAAAGT ACGTGACCGC GTCCGGACCA AGATGGAGAG AGACATCCTG 
GCTGATGTAA ATCACCCATT CGTGGTGAAG CTGCACTATG CCTTCCAGAC CGAGGGCAAG 
CTCTATCTCA TTCTGGACTT CCTGCGTGGT GGGGACCTCT TCACCCGGCT CTCAAAAGAG 
GTGATGTTCA CGGAGGAGGA TGTGAAGTTT TACCTGGCCG AGCTGGCTCT GGGCCTGGAT 
CACCTGCACA GCCTGGGTAT CATTTACAGA GACCTCAAGC CTGAGAACAT CCTTCTGGAT 
GAGGAGGGCC ACATCAAACT CACTGACTTT GGCCTGAGCA AAGAGGCCAT TGACCACGAG 
AAGAAGGCCT ATTCTTTCTG CGGGACAGTG GAGTACATGG CCCCTGAGGT CGTCAACCGC 
CAGGGCCACT CCCATAGTGC GGACTGGTGG TCCTATGGGG TGTTGATGTT TGAGATGCTG 
ACGGGCTCCC TGCCCTTCCA GGGGAAGGAC CGGAAGGAGA CCATGACACT GATTCTGAAG 
GCGAAGCTAG GCATGCCCCA GTTTCTGAGC ACTGAAGCCC AGAGCCTCTT GCGGGCCCTG 
TTCAAGCGGA ATCCTGCCAA CCGGCTCGGC TCCGGCCCTG ATGGGGCAGA GGAAATCAAG 
CGGCATGTCT TCTACTCCAC CATTGACTGG AATAAGCTAT ACCGTCGTGA GATCAAGCCA 
CCCTTCAAGC CAGCAGTGGC TCAGCCTGAT GACACCTTCT ACTTTGACAC CGAGTTCACG 
TCCCGCACAC CCAAGGATTC CCCAGGCATC CCCCCCAGCG CTGGGGCCCA TCAGCTGTTC 
CGGGGCTTCA GCTTCGTGGC CACCGGCCTG ATGGAAGACG ACGGCAAGCC TCGTGCCCCG 
CAGGCACCCC TGCACTCGGT GGTACAGCAA CTCCATGGGA AGAACCTGGT TTTTAGTGAC 
GGCTACGTGG TAAAGGAGAC AATTGGTGTG GGCTCCTACT CTGAGTGCAA GCGCTGTGTC 
CACAAGGCCA CCAACATGGA GTATGCTGTC AAGGTCATTG ATAAGAGCAA GCGGGATCCT 
TCAGAAGAGA TTGAGATTCT TCTGCGGTAT GGCCAGCACC CCAACATCAT CACTCTGAAA 
GATGTGTATG ATGATGGCAA ACACGTGTAC CTGGTGACAG AGCTGATGCG GGGTGGGGAG 
CTGCTGGACA AGATCCTGCG GCAGAAGTTC TTCTCAGAGC GGGAGGCCAG CTTTGTCCTG 
CACACCATTG GCAAAACTGT GGAGTATCTG CACTCACAGG GGGTTGTGCA CAGGGACCTG 
AAGCCCAGCA ACATCCTGTA TGTGGACGAG TCCGGGAATC CCGAGTGCCT GCGCATCTGT 
GACTTTGGTT TTGCCAAACA GCTGCGGGCT GAGAATGGGC TCCTCATGAC ACCTTGCTAC 
ACAGCCAACT TTGTGGCGCC TGAGGTGCTG AAGCGCCAGG GCTACGATGA AGGCTGCGAC 
ATCTGGAGCC TGGGCATTCT GCTGTACACC ATGCTGGCAG GATATACTCC ATTTGCCAAC 
GGTCCCAGTG ACACACCAGA GGAAATCCTA ACCCGGATCG GCAGTGGGAA GTTTACCCTC 
AGTGGGGGAA ATTGGAACAC AGTTTCAGAG ACAGCCAAGG ACCTGGTGTC CAAGATGCTA 
CACGTGGATC CCCACCAGCG CCTCACAGCT AAGCAGGTTC TGCAGCATCC ATGGGTCACC 
CAGAAAGACA AGCTTCCCCA AAGCCAGCTG TCCCACCAGG ACCTACAGCT TGTGAAGGGA 
GCCATGGCTG CCACGTACTC CGCACTCAAC AGCTCCAAGC CCACCCCCCA GCTGAAGCCC 
ATCGAGTCAT CCATCCTGGC CCAGCGGCGA GTGAGGAAGT TGCCATCCAC CACCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317370.1
CDS	51..2210
Translation	MQTPADFPRVERDLVPCPRKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL

Target ORF information:

RefSeq Version	NM_001330441.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330441.1

ORF Insert Sequence:

ATGCAGACCC CAGCAGATTT CCCCAGGGTT GAGAGAGACT TGGTTCCCTG TCCCAGAAAG 
GATGAGGGCG TCCTCAAGGA GATCTCCATC ACGCACCACG TCAAGGCTGG CTCTGAGAAG 
GCTGATCCAT CCCATTTCGA GCTCCTCAAG GTTCTGGGCC AGGGATCCTT TGGCAAAGTC 
TTCCTGGTGC GGAAAGTCAC CCGGCCTGAC AGTGGGCACC TGTATGCTAT GAAGGTGCTG 
AAGAAGGCAA CGCTGAAAGT ACGTGACCGC GTCCGGACCA AGATGGAGAG AGACATCCTG 
GCTGATGTAA ATCACCCATT CGTGGTGAAG CTGCACTATG CCTTCCAGAC CGAGGGCAAG 
CTCTATCTCA TTCTGGACTT CCTGCGTGGT GGGGACCTCT TCACCCGGCT CTCAAAAGAG 
GTGATGTTCA CGGAGGAGGA TGTGAAGTTT TACCTGGCCG AGCTGGCTCT GGGCCTGGAT 
CACCTGCACA GCCTGGGTAT CATTTACAGA GACCTCAAGC CTGAGAACAT CCTTCTGGAT 
GAGGAGGGCC ACATCAAACT CACTGACTTT GGCCTGAGCA AAGAGGCCAT TGACCACGAG 
AAGAAGGCCT ATTCTTTCTG CGGGACAGTG GAGTACATGG CCCCTGAGGT CGTCAACCGC 
CAGGGCCACT CCCATAGTGC GGACTGGTGG TCCTATGGGG TGTTGATGTT TGAGATGCTG 
ACGGGCTCCC TGCCCTTCCA GGGGAAGGAC CGGAAGGAGA CCATGACACT GATTCTGAAG 
GCGAAGCTAG GCATGCCCCA GTTTCTGAGC ACTGAAGCCC AGAGCCTCTT GCGGGCCCTG 
TTCAAGCGGA ATCCTGCCAA CCGGCTCGGC TCCGGCCCTG ATGGGGCAGA GGAAATCAAG 
CGGCATGTCT TCTACTCCAC CATTGACTGG AATAAGCTAT ACCGTCGTGA GATCAAGCCA 
CCCTTCAAGC CAGCAGTGGC TCAGCCTGAT GACACCTTCT ACTTTGACAC CGAGTTCACG 
TCCCGCACAC CCAAGGATTC CCCAGGCATC CCCCCCAGCG CTGGGGCCCA TCAGCTGTTC 
CGGGGCTTCA GCTTCGTGGC CACCGGCCTG ATGGAAGACG ACGGCAAGCC TCGTGCCCCG 
CAGGCACCCC TGCACTCGGT GGTACAGCAA CTCCATGGGA AGAACCTGGT TTTTAGTGAC 
GGCTACGTGG TAAAGGAGAC AATTGGTGTG GGCTCCTACT CTGAGTGCAA GCGCTGTGTC 
CACAAGGCCA CCAACATGGA GTATGCTGTC AAGGTCATTG ATAAGAGCAA GCGGGATCCT 
TCAGAAGAGA TTGAGATTCT TCTGCGGTAT GGCCAGCACC CCAACATCAT CACTCTGAAA 
GATGTGTATG ATGATGGCAA ACACGTGTAC CTGGTGACAG AGCTGATGCG GGGTGGGGAG 
CTGCTGGACA AGATCCTGCG GCAGAAGTTC TTCTCAGAGC GGGAGGCCAG CTTTGTCCTG 
CACACCATTG GCAAAACTGT GGAGTATCTG CACTCACAGG GGGTTGTGCA CAGGGACCTG 
AAGCCCAGCA ACATCCTGTA TGTGGACGAG TCCGGGAATC CCGAGTGCCT GCGCATCTGT 
GACTTTGGTT TTGCCAAACA GCTGCGGGCT GAGAATGGGC TCCTCATGAC ACCTTGCTAC 
ACAGCCAACT TTGTGGCGCC TGAGGTGCTG AAGCGCCAGG GCTACGATGA AGGCTGCGAC 
ATCTGGAGCC TGGGCATTCT GCTGTACACC ATGCTGGCAG GATATACTCC ATTTGCCAAC 
GGTCCCAGTG ACACACCAGA GGAAATCCTA ACCCGGATCG GCAGTGGGAA GTTTACCCTC 
AGTGGGGGAA ATTGGAACAC AGTTTCAGAG ACAGCCAAGG ACCTGGTGTC CAAGATGCTA 
CACGTGGATC CCCACCAGCG CCTCACAGCT AAGCAGGTTC TGCAGCATCC ATGGGTCACC 
CAGAAAGACA AGCTTCCCCA AAGCCAGCTG TCCCACCAGG ACCTACAGCT TGTGAAGGGA 
GCCATGGCTG CCACGTACTC CGCACTCAAC AGCTCCAAGC CCACCCCCCA GCTGAAGCCC 
ATCGAGTCAT CCATCCTGGC CCAGCGGCGA GTGAGGAAGT TGCCATCCAC CACCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS6KA1 ( NM_002953.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002944.2 Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 1, mRNA.

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CloneID	OHu107202
Clone ID Related Accession (Same CDS sequence)	NM_002953.4
Accession Version	NM_002953.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	ribosomal protein S6 kinase alpha-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627313.16. On Nov 23, 2018 this sequence version replaced NM_002953.3. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014966.1, SRR1803615.117813.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374168.7/ ENSP00000363283.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGCTCG CCCAGCTCAA GGAGCCCTGG CCGCTCATGG AGCTAGTGCC TCTGGACCCG 
GAGAATGGAC AGACCTCAGG GGAAGAAGCT GGACTTCAGC CGTCCAAGGA TGAGGGCGTC 
CTCAAGGAGA TCTCCATCAC GCACCACGTC AAGGCTGGCT CTGAGAAGGC TGATCCATCC 
CATTTCGAGC TCCTCAAGGT TCTGGGCCAG GGATCCTTTG GCAAAGTCTT CCTGGTGCGG 
AAAGTCACCC GGCCTGACAG TGGGCACCTG TATGCTATGA AGGTGCTGAA GAAGGCAACG 
CTGAAAGTAC GTGACCGCGT CCGGACCAAG ATGGAGAGAG ACATCCTGGC TGATGTAAAT 
CACCCATTCG TGGTGAAGCT GCACTATGCC TTCCAGACCG AGGGCAAGCT CTATCTCATT 
CTGGACTTCC TGCGTGGTGG GGACCTCTTC ACCCGGCTCT CAAAAGAGGT GATGTTCACG 
GAGGAGGATG TGAAGTTTTA CCTGGCTGAG CTGGCTCTGG GCCTGGATCA CCTGCACAGC 
CTGGGTATCA TTTACAGAGA CCTCAAGCCT GAGAACATCC TTCTGGATGA GGAGGGCCAC 
ATCAAACTCA CTGACTTTGG CCTGAGCAAA GAGGCCATTG ACCACGAGAA GAAGGCCTAT 
TCTTTCTGCG GGACAGTGGA GTACATGGCC CCTGAGGTCG TCAACCGCCA GGGCCACTCC 
CATAGTGCGG ACTGGTGGTC CTATGGGGTG TTGATGTTTG AGATGCTGAC GGGCTCCCTG 
CCCTTCCAGG GGAAGGACCG GAAGGAGACC ATGACACTGA TTCTGAAGGC GAAGCTAGGC 
ATGCCCCAGT TTCTGAGCAC TGAAGCCCAG AGCCTCTTGC GGGCCCTGTT CAAGCGGAAT 
CCTGCCAACC GGCTCGGCTC CGGCCCTGAT GGGGCAGAGG AAATCAAGCG GCATGTCTTC 
TACTCCACCA TTGACTGGAA TAAGCTATAC CGTCGTGAGA TCAAGCCACC CTTCAAGCCA 
GCAGTGGCTC AGCCTGATGA CACCTTCTAC TTTGACACCG AGTTCACGTC CCGCACACCC 
AAGGATTCCC CAGGCATCCC CCCCAGCGCT GGGGCCCATC AGCTGTTCCG GGGCTTCAGC 
TTCGTGGCCA CCGGCCTGAT GGAAGACGAC GGCAAGCCTC GTGCCCCGCA GGCACCCCTG 
CACTCGGTGG TACAGCAACT CCATGGGAAG AACCTGGTTT TTAGTGACGG CTACGTGGTA 
AAGGAGACAA TTGGTGTGGG CTCCTACTCT GAGTGCAAGC GCTGTGTCCA CAAGGCCACC 
AACATGGAGT ATGCTGTCAA GGTCATTGAT AAGAGCAAGC GGGATCCTTC AGAAGAGATT 
GAGATTCTTC TGCGGTATGG CCAGCACCCC AACATCATCA CTCTGAAAGA TGTGTATGAT 
GATGGCAAAC ACGTGTACCT GGTGACAGAG CTGATGCGGG GTGGGGAGCT GCTGGACAAG 
ATCCTGCGGC AGAAGTTCTT CTCAGAGCGG GAGGCCAGCT TTGTCCTGCA CACCATTGGC 
AAAACTGTGG AGTATCTGCA CTCACAGGGG GTTGTGCACA GGGACCTGAA GCCCAGCAAC 
ATCCTGTATG TGGACGAGTC CGGGAATCCC GAGTGCCTGC GCATCTGTGA CTTTGGTTTT 
GCCAAACAGC TGCGGGCTGA GAATGGGCTC CTCATGACAC CTTGCTACAC AGCCAACTTT 
GTGGCGCCTG AGGTGCTGAA GCGCCAGGGC TACGATGAAG GCTGCGACAT CTGGAGCCTG 
GGCATTCTGC TGTACACCAT GCTGGCAGGA TATACTCCAT TTGCCAACGG TCCCAGTGAC 
ACACCAGAGG AAATCCTAAC CCGGATCGGC AGTGGGAAGT TTACCCTCAG TGGGGGAAAT 
TGGAACACAG TTTCAGAGAC AGCCAAGGAC CTGGTGTCCA AGATGCTACA CGTGGATCCC 
CACCAGCGCC TCACAGCTAA GCAGGTTCTG CAGCATCCAT GGGTCACCCA GAAAGACAAG 
CTTCCCCAAA GCCAGCTGTC CCACCAGGAC CTACAGCTTG TGAAGGGAGC CATGGCTGCC 
ACGTACTCCG CACTCAACAG CTCCAAGCCC ACCCCCCAGC TGAAGCCCAT CGAGTCATCC 
ATCCTGGCCC AGCGGCGAGT GAGGAAGTTG CCATCCACCA CCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002944.2
CDS	161..2368
Translation	MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL

Target ORF information:

RefSeq Version	NM_002953.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002953.4

ORF Insert Sequence:

ATGCCGCTCG CCCAGCTCAA GGAGCCCTGG CCGCTCATGG AGCTAGTGCC TCTGGACCCG 
GAGAATGGAC AGACCTCAGG GGAAGAAGCT GGACTTCAGC CGTCCAAGGA TGAGGGCGTC 
CTCAAGGAGA TCTCCATCAC GCACCACGTC AAGGCTGGCT CTGAGAAGGC TGATCCATCC 
CATTTCGAGC TCCTCAAGGT TCTGGGCCAG GGATCCTTTG GCAAAGTCTT CCTGGTGCGG 
AAAGTCACCC GGCCTGACAG TGGGCACCTG TATGCTATGA AGGTGCTGAA GAAGGCAACG 
CTGAAAGTAC GTGACCGCGT CCGGACCAAG ATGGAGAGAG ACATCCTGGC TGATGTAAAT 
CACCCATTCG TGGTGAAGCT GCACTATGCC TTCCAGACCG AGGGCAAGCT CTATCTCATT 
CTGGACTTCC TGCGTGGTGG GGACCTCTTC ACCCGGCTCT CAAAAGAGGT GATGTTCACG 
GAGGAGGATG TGAAGTTTTA CCTGGCTGAG CTGGCTCTGG GCCTGGATCA CCTGCACAGC 
CTGGGTATCA TTTACAGAGA CCTCAAGCCT GAGAACATCC TTCTGGATGA GGAGGGCCAC 
ATCAAACTCA CTGACTTTGG CCTGAGCAAA GAGGCCATTG ACCACGAGAA GAAGGCCTAT 
TCTTTCTGCG GGACAGTGGA GTACATGGCC CCTGAGGTCG TCAACCGCCA GGGCCACTCC 
CATAGTGCGG ACTGGTGGTC CTATGGGGTG TTGATGTTTG AGATGCTGAC GGGCTCCCTG 
CCCTTCCAGG GGAAGGACCG GAAGGAGACC ATGACACTGA TTCTGAAGGC GAAGCTAGGC 
ATGCCCCAGT TTCTGAGCAC TGAAGCCCAG AGCCTCTTGC GGGCCCTGTT CAAGCGGAAT 
CCTGCCAACC GGCTCGGCTC CGGCCCTGAT GGGGCAGAGG AAATCAAGCG GCATGTCTTC 
TACTCCACCA TTGACTGGAA TAAGCTATAC CGTCGTGAGA TCAAGCCACC CTTCAAGCCA 
GCAGTGGCTC AGCCTGATGA CACCTTCTAC TTTGACACCG AGTTCACGTC CCGCACACCC 
AAGGATTCCC CAGGCATCCC CCCCAGCGCT GGGGCCCATC AGCTGTTCCG GGGCTTCAGC 
TTCGTGGCCA CCGGCCTGAT GGAAGACGAC GGCAAGCCTC GTGCCCCGCA GGCACCCCTG 
CACTCGGTGG TACAGCAACT CCATGGGAAG AACCTGGTTT TTAGTGACGG CTACGTGGTA 
AAGGAGACAA TTGGTGTGGG CTCCTACTCT GAGTGCAAGC GCTGTGTCCA CAAGGCCACC 
AACATGGAGT ATGCTGTCAA GGTCATTGAT AAGAGCAAGC GGGATCCTTC AGAAGAGATT 
GAGATTCTTC TGCGGTATGG CCAGCACCCC AACATCATCA CTCTGAAAGA TGTGTATGAT 
GATGGCAAAC ACGTGTACCT GGTGACAGAG CTGATGCGGG GTGGGGAGCT GCTGGACAAG 
ATCCTGCGGC AGAAGTTCTT CTCAGAGCGG GAGGCCAGCT TTGTCCTGCA CACCATTGGC 
AAAACTGTGG AGTATCTGCA CTCACAGGGG GTTGTGCACA GGGACCTGAA GCCCAGCAAC 
ATCCTGTATG TGGACGAGTC CGGGAATCCC GAGTGCCTGC GCATCTGTGA CTTTGGTTTT 
GCCAAACAGC TGCGGGCTGA GAATGGGCTC CTCATGACAC CTTGCTACAC AGCCAACTTT 
GTGGCGCCTG AGGTGCTGAA GCGCCAGGGC TACGATGAAG GCTGCGACAT CTGGAGCCTG 
GGCATTCTGC TGTACACCAT GCTGGCAGGA TATACTCCAT TTGCCAACGG TCCCAGTGAC 
ACACCAGAGG AAATCCTAAC CCGGATCGGC AGTGGGAAGT TTACCCTCAG TGGGGGAAAT 
TGGAACACAG TTTCAGAGAC AGCCAAGGAC CTGGTGTCCA AGATGCTACA CGTGGATCCC 
CACCAGCGCC TCACAGCTAA GCAGGTTCTG CAGCATCCAT GGGTCACCCA GAAAGACAAG 
CTTCCCCAAA GCCAGCTGTC CCACCAGGAC CTACAGCTTG TGAAGGGAGC CATGGCTGCC 
ACGTACTCCG CACTCAACAG CTCCAAGCCC ACCCCCCAGC TGAAGCCCAT CGAGTCATCC 
ATCCTGGCCC AGCGGCGAGT GAGGAAGTTG CCATCCACCA CCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS6KA1 ( NM_001006665.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001006666.1 Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 2, mRNA.

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CloneID	OHu108186
Clone ID Related Accession (Same CDS sequence)	NM_001006665.2
Accession Version	NM_001006665.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	ribosomal protein S6 kinase alpha-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627313.16. On May 31, 2019 this sequence version replaced NM_001006665.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' end of the coding region compared to variant 1. These differences cause translation initiation at an in-frame AUG of an alternate exon and an isoform (b) with a distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.115812.1, SRR1803611.103557.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGG ATCCCAAGCC GCCCCGTCTG CGGCTCTGGG CCCTGATCCC CTGGCTTCCC 
AGGAAGCAGC GGCCCAGGAT CAGCCAGACC TCTCTGCCTG TCCCTGGCCC TGGCTCTGGC 
CCCCAGCGGG ACTCGGATGA GGGCGTCCTC AAGGAGATCT CCATCACGCA CCACGTCAAG 
GCTGGCTCTG AGAAGGCTGA TCCATCCCAT TTCGAGCTCC TCAAGGTTCT GGGCCAGGGA 
TCCTTTGGCA AAGTCTTCCT GGTGCGGAAA GTCACCCGGC CTGACAGTGG GCACCTGTAT 
GCTATGAAGG TGCTGAAGAA GGCAACGCTG AAAGTACGTG ACCGCGTCCG GACCAAGATG 
GAGAGAGACA TCCTGGCTGA TGTAAATCAC CCATTCGTGG TGAAGCTGCA CTATGCCTTC 
CAGACCGAGG GCAAGCTCTA TCTCATTCTG GACTTCCTGC GTGGTGGGGA CCTCTTCACC 
CGGCTCTCAA AAGAGGTGAT GTTCACGGAG GAGGATGTGA AGTTTTACCT GGCTGAGCTG 
GCTCTGGGCC TGGATCACCT GCACAGCCTG GGTATCATTT ACAGAGACCT CAAGCCTGAG 
AACATCCTTC TGGATGAGGA GGGCCACATC AAACTCACTG ACTTTGGCCT GAGCAAAGAG 
GCCATTGACC ACGAGAAGAA GGCCTATTCT TTCTGCGGGA CAGTGGAGTA CATGGCCCCT 
GAGGTCGTCA ACCGCCAGGG CCACTCCCAT AGTGCGGACT GGTGGTCCTA TGGGGTGTTG 
ATGTTTGAGA TGCTGACGGG CTCCCTGCCC TTCCAGGGGA AGGACCGGAA GGAGACCATG 
ACACTGATTC TGAAGGCGAA GCTAGGCATG CCCCAGTTTC TGAGCACTGA AGCCCAGAGC 
CTCTTGCGGG CCCTGTTCAA GCGGAATCCT GCCAACCGGC TCGGCTCCGG CCCTGATGGG 
GCAGAGGAAA TCAAGCGGCA TGTCTTCTAC TCCACCATTG ACTGGAATAA GCTATACCGT 
CGTGAGATCA AGCCACCCTT CAAGCCAGCA GTGGCTCAGC CTGATGACAC CTTCTACTTT 
GACACCGAGT TCACGTCCCG CACACCCAAG GATTCCCCAG GCATCCCCCC CAGCGCTGGG 
GCCCATCAGC TGTTCCGGGG CTTCAGCTTC GTGGCCACCG GCCTGATGGA AGACGACGGC 
AAGCCTCGTG CCCCGCAGGC ACCCCTGCAC TCGGTGGTAC AGCAACTCCA TGGGAAGAAC 
CTGGTTTTTA GTGACGGCTA CGTGGTAAAG GAGACAATTG GTGTGGGCTC CTACTCTGAG 
TGCAAGCGCT GTGTCCACAA GGCCACCAAC ATGGAGTATG CTGTCAAGGT CATTGATAAG 
AGCAAGCGGG ATCCTTCAGA AGAGATTGAG ATTCTTCTGC GGTATGGCCA GCACCCCAAC 
ATCATCACTC TGAAAGATGT GTATGATGAT GGCAAACACG TGTACCTGGT GACAGAGCTG 
ATGCGGGGTG GGGAGCTGCT GGACAAGATC CTGCGGCAGA AGTTCTTCTC AGAGCGGGAG 
GCCAGCTTTG TCCTGCACAC CATTGGCAAA ACTGTGGAGT ATCTGCACTC ACAGGGGGTT 
GTGCACAGGG ACCTGAAGCC CAGCAACATC CTGTATGTGG ACGAGTCCGG GAATCCCGAG 
TGCCTGCGCA TCTGTGACTT TGGTTTTGCC AAACAGCTGC GGGCTGAGAA TGGGCTCCTC 
ATGACACCTT GCTACACAGC CAACTTTGTG GCGCCTGAGG TGCTGAAGCG CCAGGGCTAC 
GATGAAGGCT GCGACATCTG GAGCCTGGGC ATTCTGCTGT ACACCATGCT GGCAGGATAT 
ACTCCATTTG CCAACGGTCC CAGTGACACA CCAGAGGAAA TCCTAACCCG GATCGGCAGT 
GGGAAGTTTA CCCTCAGTGG GGGAAATTGG AACACAGTTT CAGAGACAGC CAAGGACCTG 
GTGTCCAAGA TGCTACACGT GGATCCCCAC CAGCGCCTCA CAGCTAAGCA GGTTCTGCAG 
CATCCATGGG TCACCCAGAA AGACAAGCTT CCCCAAAGCC AGCTGTCCCA CCAGGACCTA 
CAGCTTGTGA AGGGAGCCAT GGCTGCCACG TACTCCGCAC TCAACAGCTC CAAGCCCACC 
CCCCAGCTGA AGCCCATCGA GTCATCCATC CTGGCCCAGC GGCGAGTGAG GAAGTTGCCA 
TCCACCACCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006666.1
CDS	60..2294
Translation	MEQDPKPPRLRLWALIPWLPRKQRPRISQTSLPVPGPGSGPQRDSDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL

Target ORF information:

RefSeq Version	NM_001006665.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006665.2

ORF Insert Sequence:

ATGGAGCAGG ATCCCAAGCC GCCCCGTCTG CGGCTCTGGG CCCTGATCCC CTGGCTTCCC 
AGGAAGCAGC GGCCCAGGAT CAGCCAGACC TCTCTGCCTG TCCCTGGCCC TGGCTCTGGC 
CCCCAGCGGG ACTCGGATGA GGGCGTCCTC AAGGAGATCT CCATCACGCA CCACGTCAAG 
GCTGGCTCTG AGAAGGCTGA TCCATCCCAT TTCGAGCTCC TCAAGGTTCT GGGCCAGGGA 
TCCTTTGGCA AAGTCTTCCT GGTGCGGAAA GTCACCCGGC CTGACAGTGG GCACCTGTAT 
GCTATGAAGG TGCTGAAGAA GGCAACGCTG AAAGTACGTG ACCGCGTCCG GACCAAGATG 
GAGAGAGACA TCCTGGCTGA TGTAAATCAC CCATTCGTGG TGAAGCTGCA CTATGCCTTC 
CAGACCGAGG GCAAGCTCTA TCTCATTCTG GACTTCCTGC GTGGTGGGGA CCTCTTCACC 
CGGCTCTCAA AAGAGGTGAT GTTCACGGAG GAGGATGTGA AGTTTTACCT GGCTGAGCTG 
GCTCTGGGCC TGGATCACCT GCACAGCCTG GGTATCATTT ACAGAGACCT CAAGCCTGAG 
AACATCCTTC TGGATGAGGA GGGCCACATC AAACTCACTG ACTTTGGCCT GAGCAAAGAG 
GCCATTGACC ACGAGAAGAA GGCCTATTCT TTCTGCGGGA CAGTGGAGTA CATGGCCCCT 
GAGGTCGTCA ACCGCCAGGG CCACTCCCAT AGTGCGGACT GGTGGTCCTA TGGGGTGTTG 
ATGTTTGAGA TGCTGACGGG CTCCCTGCCC TTCCAGGGGA AGGACCGGAA GGAGACCATG 
ACACTGATTC TGAAGGCGAA GCTAGGCATG CCCCAGTTTC TGAGCACTGA AGCCCAGAGC 
CTCTTGCGGG CCCTGTTCAA GCGGAATCCT GCCAACCGGC TCGGCTCCGG CCCTGATGGG 
GCAGAGGAAA TCAAGCGGCA TGTCTTCTAC TCCACCATTG ACTGGAATAA GCTATACCGT 
CGTGAGATCA AGCCACCCTT CAAGCCAGCA GTGGCTCAGC CTGATGACAC CTTCTACTTT 
GACACCGAGT TCACGTCCCG CACACCCAAG GATTCCCCAG GCATCCCCCC CAGCGCTGGG 
GCCCATCAGC TGTTCCGGGG CTTCAGCTTC GTGGCCACCG GCCTGATGGA AGACGACGGC 
AAGCCTCGTG CCCCGCAGGC ACCCCTGCAC TCGGTGGTAC AGCAACTCCA TGGGAAGAAC 
CTGGTTTTTA GTGACGGCTA CGTGGTAAAG GAGACAATTG GTGTGGGCTC CTACTCTGAG 
TGCAAGCGCT GTGTCCACAA GGCCACCAAC ATGGAGTATG CTGTCAAGGT CATTGATAAG 
AGCAAGCGGG ATCCTTCAGA AGAGATTGAG ATTCTTCTGC GGTATGGCCA GCACCCCAAC 
ATCATCACTC TGAAAGATGT GTATGATGAT GGCAAACACG TGTACCTGGT GACAGAGCTG 
ATGCGGGGTG GGGAGCTGCT GGACAAGATC CTGCGGCAGA AGTTCTTCTC AGAGCGGGAG 
GCCAGCTTTG TCCTGCACAC CATTGGCAAA ACTGTGGAGT ATCTGCACTC ACAGGGGGTT 
GTGCACAGGG ACCTGAAGCC CAGCAACATC CTGTATGTGG ACGAGTCCGG GAATCCCGAG 
TGCCTGCGCA TCTGTGACTT TGGTTTTGCC AAACAGCTGC GGGCTGAGAA TGGGCTCCTC 
ATGACACCTT GCTACACAGC CAACTTTGTG GCGCCTGAGG TGCTGAAGCG CCAGGGCTAC 
GATGAAGGCT GCGACATCTG GAGCCTGGGC ATTCTGCTGT ACACCATGCT GGCAGGATAT 
ACTCCATTTG CCAACGGTCC CAGTGACACA CCAGAGGAAA TCCTAACCCG GATCGGCAGT 
GGGAAGTTTA CCCTCAGTGG GGGAAATTGG AACACAGTTT CAGAGACAGC CAAGGACCTG 
GTGTCCAAGA TGCTACACGT GGATCCCCAC CAGCGCCTCA CAGCTAAGCA GGTTCTGCAG 
CATCCATGGG TCACCCAGAA AGACAAGCTT CCCCAAAGCC AGCTGTCCCA CCAGGACCTA 
CAGCTTGTGA AGGGAGCCAT GGCTGCCACG TACTCCGCAC TCAACAGCTC CAAGCCCACC 
CCCCAGCTGA AGCCCATCGA GTCATCCATC CTGGCCCAGC GGCGAGTGAG GAAGTTGCCA 
TCCACCACCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS6KA1 ( NM_001330441.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317370.1 Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 3, mRNA.

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CloneID	OHu33681
Clone ID Related Accession (Same CDS sequence)	NM_001330441.2
Accession Version	NM_001330441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	ribosomal protein S6 kinase alpha-1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627313.16. On May 31, 2019 this sequence version replaced NM_001330441.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' end of the coding region compared to variant 1. These differences cause translation initiation at an in-frame AUG of an alternate exon and an isoform (c) with a distinct N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.278817.1, SRR1803614.187271.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGACCC CAGCAGATTT CCCCAGGGTT GAGAGAGACT TGGTTCCCTG TCCCAGAAAG 
GATGAGGGCG TCCTCAAGGA GATCTCCATC ACGCACCACG TCAAGGCTGG CTCTGAGAAG 
GCTGATCCAT CCCATTTCGA GCTCCTCAAG GTTCTGGGCC AGGGATCCTT TGGCAAAGTC 
TTCCTGGTGC GGAAAGTCAC CCGGCCTGAC AGTGGGCACC TGTATGCTAT GAAGGTGCTG 
AAGAAGGCAA CGCTGAAAGT ACGTGACCGC GTCCGGACCA AGATGGAGAG AGACATCCTG 
GCTGATGTAA ATCACCCATT CGTGGTGAAG CTGCACTATG CCTTCCAGAC CGAGGGCAAG 
CTCTATCTCA TTCTGGACTT CCTGCGTGGT GGGGACCTCT TCACCCGGCT CTCAAAAGAG 
GTGATGTTCA CGGAGGAGGA TGTGAAGTTT TACCTGGCTG AGCTGGCTCT GGGCCTGGAT 
CACCTGCACA GCCTGGGTAT CATTTACAGA GACCTCAAGC CTGAGAACAT CCTTCTGGAT 
GAGGAGGGCC ACATCAAACT CACTGACTTT GGCCTGAGCA AAGAGGCCAT TGACCACGAG 
AAGAAGGCCT ATTCTTTCTG CGGGACAGTG GAGTACATGG CCCCTGAGGT CGTCAACCGC 
CAGGGCCACT CCCATAGTGC GGACTGGTGG TCCTATGGGG TGTTGATGTT TGAGATGCTG 
ACGGGCTCCC TGCCCTTCCA GGGGAAGGAC CGGAAGGAGA CCATGACACT GATTCTGAAG 
GCGAAGCTAG GCATGCCCCA GTTTCTGAGC ACTGAAGCCC AGAGCCTCTT GCGGGCCCTG 
TTCAAGCGGA ATCCTGCCAA CCGGCTCGGC TCCGGCCCTG ATGGGGCAGA GGAAATCAAG 
CGGCATGTCT TCTACTCCAC CATTGACTGG AATAAGCTAT ACCGTCGTGA GATCAAGCCA 
CCCTTCAAGC CAGCAGTGGC TCAGCCTGAT GACACCTTCT ACTTTGACAC CGAGTTCACG 
TCCCGCACAC CCAAGGATTC CCCAGGCATC CCCCCCAGCG CTGGGGCCCA TCAGCTGTTC 
CGGGGCTTCA GCTTCGTGGC CACCGGCCTG ATGGAAGACG ACGGCAAGCC TCGTGCCCCG 
CAGGCACCCC TGCACTCGGT GGTACAGCAA CTCCATGGGA AGAACCTGGT TTTTAGTGAC 
GGCTACGTGG TAAAGGAGAC AATTGGTGTG GGCTCCTACT CTGAGTGCAA GCGCTGTGTC 
CACAAGGCCA CCAACATGGA GTATGCTGTC AAGGTCATTG ATAAGAGCAA GCGGGATCCT 
TCAGAAGAGA TTGAGATTCT TCTGCGGTAT GGCCAGCACC CCAACATCAT CACTCTGAAA 
GATGTGTATG ATGATGGCAA ACACGTGTAC CTGGTGACAG AGCTGATGCG GGGTGGGGAG 
CTGCTGGACA AGATCCTGCG GCAGAAGTTC TTCTCAGAGC GGGAGGCCAG CTTTGTCCTG 
CACACCATTG GCAAAACTGT GGAGTATCTG CACTCACAGG GGGTTGTGCA CAGGGACCTG 
AAGCCCAGCA ACATCCTGTA TGTGGACGAG TCCGGGAATC CCGAGTGCCT GCGCATCTGT 
GACTTTGGTT TTGCCAAACA GCTGCGGGCT GAGAATGGGC TCCTCATGAC ACCTTGCTAC 
ACAGCCAACT TTGTGGCGCC TGAGGTGCTG AAGCGCCAGG GCTACGATGA AGGCTGCGAC 
ATCTGGAGCC TGGGCATTCT GCTGTACACC ATGCTGGCAG GATATACTCC ATTTGCCAAC 
GGTCCCAGTG ACACACCAGA GGAAATCCTA ACCCGGATCG GCAGTGGGAA GTTTACCCTC 
AGTGGGGGAA ATTGGAACAC AGTTTCAGAG ACAGCCAAGG ACCTGGTGTC CAAGATGCTA 
CACGTGGATC CCCACCAGCG CCTCACAGCT AAGCAGGTTC TGCAGCATCC ATGGGTCACC 
CAGAAAGACA AGCTTCCCCA AAGCCAGCTG TCCCACCAGG ACCTACAGCT TGTGAAGGGA 
GCCATGGCTG CCACGTACTC CGCACTCAAC AGCTCCAAGC CCACCCCCCA GCTGAAGCCC 
ATCGAGTCAT CCATCCTGGC CCAGCGGCGA GTGAGGAAGT TGCCATCCAC CACCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317370.1
CDS	51..2210
Translation	MQTPADFPRVERDLVPCPRKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL

Target ORF information:

RefSeq Version	NM_001330441.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330441.2

ORF Insert Sequence:

ATGCAGACCC CAGCAGATTT CCCCAGGGTT GAGAGAGACT TGGTTCCCTG TCCCAGAAAG 
GATGAGGGCG TCCTCAAGGA GATCTCCATC ACGCACCACG TCAAGGCTGG CTCTGAGAAG 
GCTGATCCAT CCCATTTCGA GCTCCTCAAG GTTCTGGGCC AGGGATCCTT TGGCAAAGTC 
TTCCTGGTGC GGAAAGTCAC CCGGCCTGAC AGTGGGCACC TGTATGCTAT GAAGGTGCTG 
AAGAAGGCAA CGCTGAAAGT ACGTGACCGC GTCCGGACCA AGATGGAGAG AGACATCCTG 
GCTGATGTAA ATCACCCATT CGTGGTGAAG CTGCACTATG CCTTCCAGAC CGAGGGCAAG 
CTCTATCTCA TTCTGGACTT CCTGCGTGGT GGGGACCTCT TCACCCGGCT CTCAAAAGAG 
GTGATGTTCA CGGAGGAGGA TGTGAAGTTT TACCTGGCTG AGCTGGCTCT GGGCCTGGAT 
CACCTGCACA GCCTGGGTAT CATTTACAGA GACCTCAAGC CTGAGAACAT CCTTCTGGAT 
GAGGAGGGCC ACATCAAACT CACTGACTTT GGCCTGAGCA AAGAGGCCAT TGACCACGAG 
AAGAAGGCCT ATTCTTTCTG CGGGACAGTG GAGTACATGG CCCCTGAGGT CGTCAACCGC 
CAGGGCCACT CCCATAGTGC GGACTGGTGG TCCTATGGGG TGTTGATGTT TGAGATGCTG 
ACGGGCTCCC TGCCCTTCCA GGGGAAGGAC CGGAAGGAGA CCATGACACT GATTCTGAAG 
GCGAAGCTAG GCATGCCCCA GTTTCTGAGC ACTGAAGCCC AGAGCCTCTT GCGGGCCCTG 
TTCAAGCGGA ATCCTGCCAA CCGGCTCGGC TCCGGCCCTG ATGGGGCAGA GGAAATCAAG 
CGGCATGTCT TCTACTCCAC CATTGACTGG AATAAGCTAT ACCGTCGTGA GATCAAGCCA 
CCCTTCAAGC CAGCAGTGGC TCAGCCTGAT GACACCTTCT ACTTTGACAC CGAGTTCACG 
TCCCGCACAC CCAAGGATTC CCCAGGCATC CCCCCCAGCG CTGGGGCCCA TCAGCTGTTC 
CGGGGCTTCA GCTTCGTGGC CACCGGCCTG ATGGAAGACG ACGGCAAGCC TCGTGCCCCG 
CAGGCACCCC TGCACTCGGT GGTACAGCAA CTCCATGGGA AGAACCTGGT TTTTAGTGAC 
GGCTACGTGG TAAAGGAGAC AATTGGTGTG GGCTCCTACT CTGAGTGCAA GCGCTGTGTC 
CACAAGGCCA CCAACATGGA GTATGCTGTC AAGGTCATTG ATAAGAGCAA GCGGGATCCT 
TCAGAAGAGA TTGAGATTCT TCTGCGGTAT GGCCAGCACC CCAACATCAT CACTCTGAAA 
GATGTGTATG ATGATGGCAA ACACGTGTAC CTGGTGACAG AGCTGATGCG GGGTGGGGAG 
CTGCTGGACA AGATCCTGCG GCAGAAGTTC TTCTCAGAGC GGGAGGCCAG CTTTGTCCTG 
CACACCATTG GCAAAACTGT GGAGTATCTG CACTCACAGG GGGTTGTGCA CAGGGACCTG 
AAGCCCAGCA ACATCCTGTA TGTGGACGAG TCCGGGAATC CCGAGTGCCT GCGCATCTGT 
GACTTTGGTT TTGCCAAACA GCTGCGGGCT GAGAATGGGC TCCTCATGAC ACCTTGCTAC 
ACAGCCAACT TTGTGGCGCC TGAGGTGCTG AAGCGCCAGG GCTACGATGA AGGCTGCGAC 
ATCTGGAGCC TGGGCATTCT GCTGTACACC ATGCTGGCAG GATATACTCC ATTTGCCAAC 
GGTCCCAGTG ACACACCAGA GGAAATCCTA ACCCGGATCG GCAGTGGGAA GTTTACCCTC 
AGTGGGGGAA ATTGGAACAC AGTTTCAGAG ACAGCCAAGG ACCTGGTGTC CAAGATGCTA 
CACGTGGATC CCCACCAGCG CCTCACAGCT AAGCAGGTTC TGCAGCATCC ATGGGTCACC 
CAGAAAGACA AGCTTCCCCA AAGCCAGCTG TCCCACCAGG ACCTACAGCT TGTGAAGGGA 
GCCATGGCTG CCACGTACTC CGCACTCAAC AGCTCCAAGC CCACCCCCCA GCTGAAGCCC 
ATCGAGTCAT CCATCCTGGC CCAGCGGCGA GTGAGGAAGT TGCCATCCAC CACCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS6KA1 ( XM_024448871.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304639.1 Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant X1, mRNA.

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CloneID	OHu33682
Clone ID Related Accession (Same CDS sequence)	XM_024448871.1
Accession Version	XM_024448871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ribosomal protein S6 kinase alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGC TGAAGAAGGC AACGCTGAAA GTACGTGACC GCGTCCGGAC CAAGATGGAG 
AGAGACATCC TGGCTGATGT AAATCACCCA TTCGTGGTGA AGCTGCACTA TGCCTTCCAG 
ACCGAGGGCA AGCTCTATCT CATTCTGGAC TTCCTGCGTG GTGGGGACCT CTTCACCCGG 
CTCTCAAAAG AGGTGATGTT CACGGAGGAG GATGTGAAGT TTTACCTGGC TGAGCTGGCT 
CTGGGCCTGG ATCACCTGCA CAGCCTGGGT ATCATTTACA GAGACCTCAA GCCTGAGAAC 
ATCCTTCTGG ATGAGGAGGG CCACATCAAA CTCACTGACT TTGGCCTGAG CAAAGAGGCC 
ATTGACCACG AGAAGAAGGC CTATTCTTTC TGCGGGACAG TGGAGTACAT GGCCCCTGAG 
GTCGTCAACC GCCAGGGCCA CTCCCATAGT GCGGACTGGT GGTCCTATGG GGTGTTGATG 
TTTGAGATGC TGACGGGCTC CCTGCCCTTC CAGGGGAAGG ACCGGAAGGA GACCATGACA 
CTGATTCTGA AGGCGAAGCT AGGCATGCCC CAGTTTCTGA GCACTGAAGC CCAGAGCCTC 
TTGCGGGCCC TGTTCAAGCG GAATCCTGCC AACCGGCTCG GCTCCGGCCC TGATGGGGCA 
GAGGAAATCA AGCGGCATGT CTTCTACTCC ACCATTGACT GGAATAAGCT ATACCGTCGT 
GAGATCAAGC CACCCTTCAA GCCAGCAGTG GCTCAGCCTG ATGACACCTT CTACTTTGAC 
ACCGAGTTCA CGTCCCGCAC ACCCAAGGAT TCCCCAGGCA TCCCCCCCAG CGCTGGGGCC 
CATCAGCTGT TCCGGGGCTT CAGCTTCGTG GCCACCGGCC TGATGGAAGA CGACGGCAAG 
CCTCGTGCCC CGCAGGCACC CCTGCACTCG GTGGTACAGC AACTCCATGG GAAGAACCTG 
GTTTTTAGTG ACGGCTACGT GGTAAAGGAG ACAATTGGTG TGGGCTCCTA CTCTGAGTGC 
AAGCGCTGTG TCCACAAGGC CACCAACATG GAGTATGCTG TCAAGGTCAT TGATAAGAGC 
AAGCGGGATC CTTCAGAAGA GATTGAGATT CTTCTGCGGT ATGGCCAGCA CCCCAACATC 
ATCACTCTGA AAGATGTGTA TGATGATGGC AAACACGTGT ACCTGGTGAC AGAGCTGATG 
CGGGGTGGGG AGCTGCTGGA CAAGATCCTG CGGCAGAAGT TCTTCTCAGA GCGGGAGGCC 
AGCTTTGTCC TGCACACCAT TGGCAAAACT GTGGAGTATC TGCACTCACA GGGGGTTGTG 
CACAGGGACC TGAAGCCCAG CAACATCCTG TATGTGGACG AGTCCGGGAA TCCCGAGTGC 
CTGCGCATCT GTGACTTTGG TTTTGCCAAA CAGCTGCGGG CTGAGAATGG GCTCCTCATG 
ACACCTTGCT ACACAGCCAA CTTTGTGGCG CCTGAGGTGC TGAAGCGCCA GGGCTACGAT 
GAAGGCTGCG ACATCTGGAG CCTGGGCATT CTGCTGTACA CCATGCTGGC AGGATATACT 
CCATTTGCCA ACGGTCCCAG TGACACACCA GAGGAAATCC TAACCCGGAT CGGCAGTGGG 
AAGTTTACCC TCAGTGGGGG AAATTGGAAC ACAGTTTCAG AGACAGCCAA GGACCTGGTG 
TCCAAGATGC TACACGTGGA TCCCCACCAG CGCCTCACAG CTAAGCAGGT TCTGCAGCAT 
CCATGGGTCA CCCAGAAAGA CAAGCTTCCC CAAAGCCAGC TGTCCCACCA GGACCTACAG 
CTTGTGAAGG GAGCCATGGC TGCCACGTAC TCCGCACTCA ACAGCTCCAA GCCCACCCCC 
CAGCTGAAGC CCATCGAGTC ATCCATCCTG GCCCAGCGGC GAGTGAGGAA GTTGCCATCC 
ACCACCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304639.1
CDS	430..2361
Translation	MKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL

Target ORF information:

RefSeq Version	XM_024448871.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S6 kinase A1 (RPS6KA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448871.1

ORF Insert Sequence:

ATGAAGGTGC TGAAGAAGGC AACGCTGAAA GTACGTGACC GCGTCCGGAC CAAGATGGAG 
AGAGACATCC TGGCTGATGT AAATCACCCA TTCGTGGTGA AGCTGCACTA TGCCTTCCAG 
ACCGAGGGCA AGCTCTATCT CATTCTGGAC TTCCTGCGTG GTGGGGACCT CTTCACCCGG 
CTCTCAAAAG AGGTGATGTT CACGGAGGAG GATGTGAAGT TTTACCTGGC TGAGCTGGCT 
CTGGGCCTGG ATCACCTGCA CAGCCTGGGT ATCATTTACA GAGACCTCAA GCCTGAGAAC 
ATCCTTCTGG ATGAGGAGGG CCACATCAAA CTCACTGACT TTGGCCTGAG CAAAGAGGCC 
ATTGACCACG AGAAGAAGGC CTATTCTTTC TGCGGGACAG TGGAGTACAT GGCCCCTGAG 
GTCGTCAACC GCCAGGGCCA CTCCCATAGT GCGGACTGGT GGTCCTATGG GGTGTTGATG 
TTTGAGATGC TGACGGGCTC CCTGCCCTTC CAGGGGAAGG ACCGGAAGGA GACCATGACA 
CTGATTCTGA AGGCGAAGCT AGGCATGCCC CAGTTTCTGA GCACTGAAGC CCAGAGCCTC 
TTGCGGGCCC TGTTCAAGCG GAATCCTGCC AACCGGCTCG GCTCCGGCCC TGATGGGGCA 
GAGGAAATCA AGCGGCATGT CTTCTACTCC ACCATTGACT GGAATAAGCT ATACCGTCGT 
GAGATCAAGC CACCCTTCAA GCCAGCAGTG GCTCAGCCTG ATGACACCTT CTACTTTGAC 
ACCGAGTTCA CGTCCCGCAC ACCCAAGGAT TCCCCAGGCA TCCCCCCCAG CGCTGGGGCC 
CATCAGCTGT TCCGGGGCTT CAGCTTCGTG GCCACCGGCC TGATGGAAGA CGACGGCAAG 
CCTCGTGCCC CGCAGGCACC CCTGCACTCG GTGGTACAGC AACTCCATGG GAAGAACCTG 
GTTTTTAGTG ACGGCTACGT GGTAAAGGAG ACAATTGGTG TGGGCTCCTA CTCTGAGTGC 
AAGCGCTGTG TCCACAAGGC CACCAACATG GAGTATGCTG TCAAGGTCAT TGATAAGAGC 
AAGCGGGATC CTTCAGAAGA GATTGAGATT CTTCTGCGGT ATGGCCAGCA CCCCAACATC 
ATCACTCTGA AAGATGTGTA TGATGATGGC AAACACGTGT ACCTGGTGAC AGAGCTGATG 
CGGGGTGGGG AGCTGCTGGA CAAGATCCTG CGGCAGAAGT TCTTCTCAGA GCGGGAGGCC 
AGCTTTGTCC TGCACACCAT TGGCAAAACT GTGGAGTATC TGCACTCACA GGGGGTTGTG 
CACAGGGACC TGAAGCCCAG CAACATCCTG TATGTGGACG AGTCCGGGAA TCCCGAGTGC 
CTGCGCATCT GTGACTTTGG TTTTGCCAAA CAGCTGCGGG CTGAGAATGG GCTCCTCATG 
ACACCTTGCT ACACAGCCAA CTTTGTGGCG CCTGAGGTGC TGAAGCGCCA GGGCTACGAT 
GAAGGCTGCG ACATCTGGAG CCTGGGCATT CTGCTGTACA CCATGCTGGC AGGATATACT 
CCATTTGCCA ACGGTCCCAG TGACACACCA GAGGAAATCC TAACCCGGAT CGGCAGTGGG 
AAGTTTACCC TCAGTGGGGG AAATTGGAAC ACAGTTTCAG AGACAGCCAA GGACCTGGTG 
TCCAAGATGC TACACGTGGA TCCCCACCAG CGCCTCACAG CTAAGCAGGT TCTGCAGCAT 
CCATGGGTCA CCCAGAAAGA CAAGCTTCCC CAAAGCCAGC TGTCCCACCA GGACCTACAG 
CTTGTGAAGG GAGCCATGGC TGCCACGTAC TCCGCACTCA ACAGCTCCAA GCCCACCCCC 
CAGCTGAAGC CCATCGAGTC ATCCATCCTG GCCCAGCGGC GAGTGAGGAA GTTGCCATCC 
ACCACCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

L1 coupling to ankyrin and the spectrin-actin cytoskeleton modulates ethanol inhibition of L1 adhesion and ethanol teratogenesis.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Nov)
Dou X,Menkari C,Mitsuyama R,Foroud T,Wetherill L,Hammond P,Suttie M,Chen X,Chen SY,Charness ME,

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RPS6KA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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