RPS5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPS5
Entrez Gene ID	6193
Full Name	ribosomal protein S5
Synonyms	S5
General protein information	Preferred Names ribosomal protein S5 Names 40S ribosomal protein S5 small ribosomal subunit protein uS7
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.43
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S7P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008].
Disorder MIM:	603630

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009.4	NP_001000.2	40S ribosomal protein S5
NM_001009.3	NP_001000.2	40S ribosomal protein S5

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RPS5	NP_001015531.1
Mus musculus (house mouse)	Rps5	NP_033121.2
Eremothecium gossypii	AGOS_ABR171W	NP_983119.1
Arabidopsis thaliana (thale cress)	RPS5A	NP_187800.1
Arabidopsis thaliana (thale cress)	RPS5B	NP_181264.1
Pan troglodytes (chimpanzee)	RPS5	XP_001147137.2
	RPS5	XP_001097103.1
Schizosaccharomyces pombe (fission yeast)	rps5	NP_594279.1
Magnaporthe oryzae (rice blast fungus)	MGG_06658	XP_003709365.1
Neurospora crassa	NCU09475	XP_963230.2
Canis lupus familiaris (dog)	RPS5	XP_533568.1
Xenopus tropicalis (tropical clawed frog)	rps5	NP_001016992.1
Rattus norvegicus (Norway rat)	Rps5	NP_001264172.1
Drosophila melanogaster (fruit fly)	RpS5a	NP_523382.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008329	XP_555129.1
Caenorhabditis elegans (roundworm)	rps-5	NP_502077.1
Schizosaccharomyces pombe (fission yeast)	rps502	NP_594212.1
Oryza sativa (rice)	Os01g0100700	NP_001041738.1
Oryza sativa (rice)	Os11g0482000	NP_001067900.1
Homo sapiens (human)	RPS5	NP_001000.2
Danio rerio (zebrafish)	rps5	NP_775339.1
Saccharomyces cerevisiae (baker's yeast)	RPS5	NP_012657.1
Kluyveromyces lactis	KLLA0D10659g	XP_453536.1

Gene Ontology
Bibliography

Related articles in PubMed

The beta hairpin structure within ribosomal protein S5 mediates interplay between domains II and IV and regulates HCV IRES function.
Bhat P, Shwetha S, Sharma DK, Joseph AP, Srinivasan N, Das S
Nucleic acids research43(5)2888-901(2015 Mar)

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP
Journal of virology88(18)10340-53(2014 Sep)

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Zanivan S, Maione F, Hein MY, Hern?ndez-Fernaud JR, Ostasiewicz P, Giraudo E, Mann M
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that beta hairpin structure present in ribosomal protein S5 (RPS5) is important for IRES-RPS5 interaction.
Title: The beta hairpin structure within ribosomal protein S5 mediates interplay between domains II and IV and regulates HCV IRES function.

Mutual effect of human ribosomal proteins S5 and S16 on their binding with 18S rRNA fragment 1203-1236/1521-1698
Title: [Mutual effect of human ribosomal proteins S5 and S16 on their binding with 18S rRNA fragment 1203-1236/1521-1698].

Transfected into yeast cells, plays an important role in maintaining the accuracy of translation.
Title: Roles of the negatively charged N-terminal extension of Saccharomyces cerevisiae ribosomal protein S5 revealed by characterization of a yeast strain containing human ribosomal protein S5.

The following RPS5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPS5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31168	NM_001009.4 Latest version!	Homo sapiens ribosomal protein S5 (RPS5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu31168	NM_001009.3	Homo sapiens ribosomal protein S5 (RPS5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPS5 ( NM_001009.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001000.2 Homo sapiens ribosomal protein S5 (RPS5), mRNA.

Price & Availability↑

CloneID	OHu31168
Clone ID Related Accession (Same CDS sequence)	NM_001009.4 , NM_001009.3
Accession Version	NM_001009.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	40S ribosomal protein S5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BG687415.1 and BC015405.1. On Nov 23, 2018 this sequence version replaced NM_001009.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189652.100764.1, SRR5189658.142489.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000196551.8/ ENSP00000196551.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCGAGT GGGAGACAGC AGCACCAGCG GTGGCAGAGA CCCCAGACAT CAAGCTCTTT 
GGGAAGTGGA GCACCGATGA TGTGCAGATC AATGACATTT CCCTGCAGGA TTACATTGCA 
GTGAAGGAGA AGTATGCCAA GTACCTGCCT CACAGTGCAG GGCGGTATGC CGCCAAACGC 
TTCCGCAAAG CTCAGTGTCC CATTGTGGAG CGCCTCACTA ACTCCATGAT GATGCACGGC 
CGCAACAACG GCAAGAAGCT CATGACTGTG CGCATCGTCA AGCATGCCTT CGAGATCATA 
CACCTGCTCA CAGGCGAGAA CCCTCTGCAG GTCCTGGTGA ACGCCATCAT CAACAGTGGT 
CCCCGGGAGG ACTCCACACG CATTGGGCGC GCCGGGACTG TGAGACGACA GGCTGTGGAT 
GTGTCCCCCC TGCGCCGTGT GAACCAGGCC ATCTGGCTGC TGTGCACAGG CGCTCGTGAG 
GCTGCCTTCC GGAACATTAA GACCATTGCT GAGTGCCTGG CAGATGAGCT CATCAATGCT 
GCCAAGGGCT CCTCGAACTC CTATGCCATT AAGAAGAAGG ACGAGCTGGA GCGTGTGGCC 
AAGTCCAACC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001000.2
CDS	73..687
Translation	MTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHGRNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR

Target ORF information:

RefSeq Version	NM_001009.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S5 (RPS5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009.4

ORF Insert Sequence:

ATGACCGAGT GGGAGACAGC AGCACCAGCG GTGGCAGAGA CCCCAGACAT CAAGCTCTTT 
GGGAAGTGGA GCACCGATGA TGTGCAGATC AATGACATTT CCCTGCAGGA TTACATTGCA 
GTGAAGGAGA AGTATGCCAA GTACCTGCCT CACAGTGCAG GGCGGTATGC CGCCAAACGC 
TTCCGCAAAG CTCAGTGTCC CATTGTGGAG CGCCTCACTA ACTCCATGAT GATGCACGGC 
CGCAACAACG GCAAGAAGCT CATGACTGTG CGCATCGTCA AGCATGCCTT CGAGATCATA 
CACCTGCTCA CAGGCGAGAA CCCTCTGCAG GTCCTGGTGA ACGCCATCAT CAACAGTGGT 
CCCCGGGAGG ACTCCACACG CATTGGGCGC GCCGGGACTG TGAGACGACA GGCTGTGGAT 
GTGTCCCCCC TGCGCCGTGT GAACCAGGCC ATCTGGCTGC TGTGCACAGG CGCTCGTGAG 
GCTGCCTTCC GGAACATTAA GACCATTGCT GAGTGCCTGG CAGATGAGCT CATCAATGCT 
GCCAAGGGCT CCTCGAACTC CTATGCCATT AAGAAGAAGG ACGAGCTGGA GCGTGTGGCC 
AAGTCCAACC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS5 ( NM_001009.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001000.2 Homo sapiens ribosomal protein S5 (RPS5), mRNA.

Price & Availability↑

CloneID	OHu31168
Clone ID Related Accession (Same CDS sequence)	NM_001009.4 , NM_001009.3
Accession Version	NM_001009.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	40S ribosomal protein S5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BG687415.1 and BC015405.1. On Jul 26, 2005 this sequence version replaced NM_001009.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223106.1, CD048050.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGACCGAGT GGGAGACAGC AGCACCAGCG GTGGCAGAGA CCCCAGACAT CAAGCTCTTT 
GGGAAGTGGA GCACCGATGA TGTGCAGATC AATGACATTT CCCTGCAGGA TTACATTGCA 
GTGAAGGAGA AGTATGCCAA GTACCTGCCT CACAGTGCAG GGCGGTATGC CGCCAAACGC 
TTCCGCAAAG CTCAGTGTCC CATTGTGGAG CGCCTCACTA ACTCCATGAT GATGCACGGC 
CGCAACAACG GCAAGAAGCT CATGACTGTG CGCATCGTCA AGCATGCCTT CGAGATCATA 
CACCTGCTCA CAGGCGAGAA CCCTCTGCAG GTCCTGGTGA ACGCCATCAT CAACAGTGGT 
CCCCGGGAGG ACTCCACACG CATTGGGCGC GCCGGGACTG TGAGACGACA GGCTGTGGAT 
GTGTCCCCCC TGCGCCGTGT GAACCAGGCC ATCTGGCTGC TGTGCACAGG CGCTCGTGAG 
GCTGCCTTCC GGAACATTAA GACCATTGCT GAGTGCCTGG CAGATGAGCT CATCAATGCT 
GCCAAGGGCT CCTCGAACTC CTATGCCATT AAGAAGAAGG ACGAGCTGGA GCGTGTGGCC 
AAGTCCAACC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001000.2
CDS	73..687
Translation	MTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHGRNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR

Target ORF information:

RefSeq Version	NM_001009.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein S5 (RPS5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009.3

ORF Insert Sequence:

ATGACCGAGT GGGAGACAGC AGCACCAGCG GTGGCAGAGA CCCCAGACAT CAAGCTCTTT 
GGGAAGTGGA GCACCGATGA TGTGCAGATC AATGACATTT CCCTGCAGGA TTACATTGCA 
GTGAAGGAGA AGTATGCCAA GTACCTGCCT CACAGTGCAG GGCGGTATGC CGCCAAACGC 
TTCCGCAAAG CTCAGTGTCC CATTGTGGAG CGCCTCACTA ACTCCATGAT GATGCACGGC 
CGCAACAACG GCAAGAAGCT CATGACTGTG CGCATCGTCA AGCATGCCTT CGAGATCATA 
CACCTGCTCA CAGGCGAGAA CCCTCTGCAG GTCCTGGTGA ACGCCATCAT CAACAGTGGT 
CCCCGGGAGG ACTCCACACG CATTGGGCGC GCCGGGACTG TGAGACGACA GGCTGTGGAT 
GTGTCCCCCC TGCGCCGTGT GAACCAGGCC ATCTGGCTGC TGTGCACAGG CGCTCGTGAG 
GCTGCCTTCC GGAACATTAA GACCATTGCT GAGTGCCTGG CAGATGAGCT CATCAATGCT 
GCCAAGGGCT CCTCGAACTC CTATGCCATT AAGAAGAAGG ACGAGCTGGA GCGTGTGGCC 
AAGTCCAACC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The beta hairpin structure within ribosomal protein S5 mediates interplay between domains II and IV and regulates HCV IRES function.
Nucleic acids research43(5)2888-901(2015 Mar)
Bhat P,Shwetha S,Sharma DK,Joseph AP,Srinivasan N,Das S

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Journal of virology88(18)10340-53(2014 Sep)
Jefferson M,Donaszi-Ivanov A,Pollen S,Dalmay T,Saalbach G,Powell PP

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)
Zanivan S,Maione F,Hein MY,Hern?ndez-Fernaud JR,Ostasiewicz P,Giraudo E,Mann M

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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RPS5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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