MRPL12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPL12
Entrez Gene ID	6182
Full Name	mitochondrial ribosomal protein L12
Synonyms	5c5-2,L12mt,MRP-L31/34,MRPL7,MRPL7/L12,RPML12
General protein information	Preferred Names mitochondrial ribosomal protein L12 Names 39S ribosomal protein L12, mitochondrial MRP-L12 mitochondrial large ribosomal subunit protein bL12m
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.3
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq, Jul 2008].
Disorder MIM:	602375

mRNA and Protein(s)

mRNA	Protein	Name
NM_002949.3	NP_002940.2	39S ribosomal protein L12, mitochondrial
NM_002949.4	NP_002940.2	39S ribosomal protein L12, mitochondrial

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MRPL12	NP_963900.1
Mus musculus (house mouse)	Mrpl12	NP_081480.2
Neurospora crassa	NCU06469	XP_957360.1
Pan troglodytes (chimpanzee)	MRPL12	XP_001163363.1
Canis lupus familiaris (dog)	MRPL12	XP_850016.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011581	XP_309929.3
Gallus gallus (chicken)	MRPL12	XP_001232214.2
Drosophila melanogaster (fruit fly)	mRpL12	NP_524742.1
Caenorhabditis elegans (roundworm)	mrpl-12	NP_499360.1
Saccharomyces cerevisiae (baker's yeast)	MNP1	NP_011447.1
Kluyveromyces lactis	KLLA0D03212g	XP_453209.1
Magnaporthe oryzae (rice blast fungus)	MGG_06378	XP_003717196.1
Xenopus tropicalis (tropical clawed frog)	mrpl12	NP_001006886.1
Danio rerio (zebrafish)	mrpl12	NP_001017696.1
Schizosaccharomyces pombe (fission yeast)	SPCC4B3.09c	NP_588081.2
Homo sapiens (human)	MRPL12	NP_002940.2
	MRPL12	XP_001111712.1
Rattus norvegicus (Norway rat)	Mrpl12	NP_001025071.1
Eremothecium gossypii	AGOS_AFL089C	NP_985459.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.
Nouws J, Goswami AV, Bestwick M, McCann BJ, Surovtseva YV, Shadel GS
The Journal of biological chemistry291(2)989-97(2016 Jan)

Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.
Serre V, Rozanska A, Beinat M, Chretien D, Boddaert N, Munnich A, R?tig A, Chrzanowska-Lightowlers ZM
Biochimica et biophysica acta1832(8)1304-12(2013 Aug)

Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.
Surovtseva YV, Shutt TE, Cotney J, Cimen H, Chen SY, Koc EC, Shadel GS
Proceedings of the National Academy of Sciences of the United States of America108(44)17921-6(2011 Nov)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

knock-down of MRPL12 by RNA interference results in instability of POLRMT.
Title: Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.

This mutation resulted in a decreased steady-state level of MRPL12 protein.
Title: Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

the MRPL12 interaction with POLRMT is likely part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation
Title: Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression.

The following MRPL12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPL12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55740	NM_002949.4 Latest version!	Homo sapiens mitochondrial ribosomal protein L12 (MRPL12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu55740	NM_002949.3	Homo sapiens mitochondrial ribosomal protein L12 (MRPL12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPL12 ( NM_002949.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002940.2 Homo sapiens mitochondrial ribosomal protein L12 (MRPL12), mRNA.

Price & Availability↑

CloneID	OHu55740
Clone ID Related Accession (Same CDS sequence)	NM_002949.3 , NM_002949.4
Accession Version	NM_002949.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	39S ribosomal protein L12, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA648796.1, BC002344.2, AF105278.1 and CA310089.1. On Nov 18, 2010 this sequence version replaced NM_002949.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.291681.1, SRR1163657.422680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCCGG CGGCCGCTCG CCCCCTGTGG GGGCCTTGCC TTGGGCTTCG GGCCGCTGCG 
TTCCGCCTTG CCAGGCGACA GGTGCCATGT GTCTGTGCCG TGCGACATAT GAGGAGCAGC 
GGCCATCAGA GGTGTGAGGC CCTCGCTGGT GCACCCCTGG ATAACGCCCC CAAGGAGTAC 
CCCCCCAAGA TACAGCAGCT GGTCCAGGAC ATCGCCAGCC TCACTCTCTT GGAAATCTCA 
GACCTCAACG AGCTCCTGAA GAAAACGTTG AAGATCCAGG ATGTCGGGCT TGTGCCGATG 
GGTGGTGTGA TGTCTGGGGC TGTCCCTGCT GCAGCAGCCC AGGAGGCGGT GGAAGAAGAT 
ATCCCCATAG CGAAAGAACG GACACATTTC ACCGTCCGCC TGACCGAGGC GAAGCCCGTG 
GACAAAGTGA AGCTGATCAA GGAAATCAAG AACTACATCC AAGGCATCAA CCTCGTCCAG 
GCAAAGAAGC TGGTGGAGTC CCTGCCCCAG GAAATCAAAG CCAATGTCGC CAAAGCTGAG 
GCGGAGAAGA TCAAGGCGGC CCTGGAGGCG GTGGGCGGCA CCGTGGTTCT GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002940.2
CDS	133..729
Translation	MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVRHMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE

Target ORF information:

RefSeq Version	NM_002949.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L12 (MRPL12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002949.3

ORF Insert Sequence:

ATGCTGCCGG CGGCCGCTCG CCCCCTGTGG GGGCCTTGCC TTGGGCTTCG GGCCGCTGCG 
TTCCGCCTTG CCAGGCGACA GGTGCCATGT GTCTGTGCCG TGCGACATAT GAGGAGCAGC 
GGCCATCAGA GGTGTGAGGC CCTCGCTGGT GCACCCCTGG ATAACGCCCC CAAGGAGTAC 
CCCCCCAAGA TACAGCAGCT GGTCCAGGAC ATCGCCAGCC TCACTCTCTT GGAAATCTCA 
GACCTCAACG AGCTCCTGAA GAAAACGTTG AAGATCCAGG ATGTCGGGCT TGTGCCGATG 
GGTGGTGTGA TGTCTGGGGC TGTCCCTGCT GCAGCAGCCC AGGAGGCGGT GGAAGAAGAT 
ATCCCCATAG CGAAAGAACG GACACATTTC ACCGTCCGCC TGACCGAGGC GAAGCCCGTG 
GACAAAGTGA AGCTGATCAA GGAAATCAAG AACTACATCC AAGGCATCAA CCTCGTCCAG 
GCAAAGAAGC TGGTGGAGTC CCTGCCCCAG GAAATCAAAG CCAATGTCGC CAAAGCTGAG 
GCGGAGAAGA TCAAGGCGGC CCTGGAGGCG GTGGGCGGCA CCGTGGTTCT GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL12 ( NM_002949.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002940.2 Homo sapiens mitochondrial ribosomal protein L12 (MRPL12), mRNA.

Price & Availability↑

CloneID	OHu55740
Clone ID Related Accession (Same CDS sequence)	NM_002949.3 , NM_002949.4
Accession Version	NM_002949.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	39S ribosomal protein L12, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC139530.3, DA648796.1, BC002344.2 and AF105278.1. On Nov 22, 2018 this sequence version replaced NM_002949.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.348680.1, SRR1803613.225094.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000333676.8/ ENSP00000333837.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCCGG CGGCCGCTCG CCCCCTGTGG GGGCCTTGCC TTGGGCTTCG GGCCGCTGCG 
TTCCGCCTTG CCAGGCGACA GGTGCCATGT GTCTGTGCCG TGCGACATAT GAGGAGCAGC 
GGCCATCAGA GGTGTGAGGC CCTCGCTGGT GCACCCCTGG ATAACGCCCC CAAGGAGTAC 
CCCCCCAAGA TACAGCAGCT GGTCCAGGAC ATCGCCAGCC TCACTCTCTT GGAAATCTCA 
GACCTCAACG AGCTCCTGAA GAAAACGTTG AAGATCCAGG ATGTCGGGCT TGTGCCGATG 
GGTGGTGTGA TGTCTGGGGC TGTCCCTGCT GCAGCAGCCC AGGAGGCGGT GGAAGAAGAT 
ATCCCCATAG CGAAAGAACG GACACATTTC ACCGTCCGCC TGACCGAGGC GAAGCCCGTG 
GACAAAGTGA AGCTGATCAA GGAAATCAAG AACTACATCC AAGGCATCAA CCTCGTCCAG 
GCAAAGAAGC TGGTGGAGTC CCTGCCCCAG GAAATCAAAG CCAATGTCGC CAAAGCTGAG 
GCGGAGAAGA TCAAGGCGGC CCTGGAGGCG GTGGGCGGCA CCGTGGTTCT GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002940.2
CDS	136..732
Translation	MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVRHMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE

Target ORF information:

RefSeq Version	NM_002949.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L12 (MRPL12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002949.4

ORF Insert Sequence:

ATGCTGCCGG CGGCCGCTCG CCCCCTGTGG GGGCCTTGCC TTGGGCTTCG GGCCGCTGCG 
TTCCGCCTTG CCAGGCGACA GGTGCCATGT GTCTGTGCCG TGCGACATAT GAGGAGCAGC 
GGCCATCAGA GGTGTGAGGC CCTCGCTGGT GCACCCCTGG ATAACGCCCC CAAGGAGTAC 
CCCCCCAAGA TACAGCAGCT GGTCCAGGAC ATCGCCAGCC TCACTCTCTT GGAAATCTCA 
GACCTCAACG AGCTCCTGAA GAAAACGTTG AAGATCCAGG ATGTCGGGCT TGTGCCGATG 
GGTGGTGTGA TGTCTGGGGC TGTCCCTGCT GCAGCAGCCC AGGAGGCGGT GGAAGAAGAT 
ATCCCCATAG CGAAAGAACG GACACATTTC ACCGTCCGCC TGACCGAGGC GAAGCCCGTG 
GACAAAGTGA AGCTGATCAA GGAAATCAAG AACTACATCC AAGGCATCAA CCTCGTCCAG 
GCAAAGAAGC TGGTGGAGTC CCTGCCCCAG GAAATCAAAG CCAATGTCGC CAAAGCTGAG 
GCGGAGAAGA TCAAGGCGGC CCTGGAGGCG GTGGGCGGCA CCGTGGTTCT GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.
The Journal of biological chemistry291(2)989-97(2016 Jan)
Nouws J,Goswami AV,Bestwick M,McCann BJ,Surovtseva YV,Shadel GS

Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.
Biochimica et biophysica acta1832(8)1304-12(2013 Aug)
Serre V,Rozanska A,Beinat M,Chretien D,Boddaert N,Munnich A,R?tig A,Chrzanowska-Lightowlers ZM

Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.
Proceedings of the National Academy of Sciences of the United States of America108(44)17921-6(2011 Nov)
Surovtseva YV,Shutt TE,Cotney J,Cimen H,Chen SY,Koc EC,Shadel GS

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

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MRPL12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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