RPL30 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPL30
Entrez Gene ID	6156
Full Name	ribosomal protein L30
Synonyms	L30
General protein information	Preferred Names ribosomal protein L30 Names 60S ribosomal protein L30 large ribosomal subunit protein eL30
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q22.2
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008].
Disorder MIM:	180467

mRNA and Protein(s)

mRNA	Protein	Name
NM_000989.4	NP_000980.1	60S ribosomal protein L30
NM_000989.3	NP_000980.1	60S ribosomal protein L30

Pathways from BioSystems

Homologies

	LOC703120	XP_001094073.1
Bos taurus (cattle)	RPL30	NP_001029606.1
Rattus norvegicus (Norway rat)	Rpl30	NP_073190.1
Schizosaccharomyces pombe (fission yeast)	rpl3002	NP_594857.1
Oryza sativa (rice)	Os01g0276000	NP_001042724.1
Xenopus tropicalis (tropical clawed frog)	MGC89963	NP_001005110.1
Rattus norvegicus (Norway rat)	LOC100362027	XP_002727755.1
Danio rerio (zebrafish)	rpl30	NP_956322.1
Magnaporthe oryzae (rice blast fungus)	MGG_03135	XP_003716863.1
Oryza sativa (rice)	Os05g0490100	NP_001055897.1
Canis lupus familiaris (dog)	RPL30	XP_537871.1
Mus musculus (house mouse)	Rpl30	NP_001156957.1
Neurospora crassa	NCU08963	XP_960785.1
Arabidopsis thaliana (thale cress)	AT1G36240	NP_174853.1
Arabidopsis thaliana (thale cress)	AT1G77940	NP_565164.1
Oryza sativa (rice)	Os01g0812800	NP_001044595.1
Homo sapiens (human)	RPL30	NP_000980.1
Gallus gallus (chicken)	RPL30	NP_001007968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003816	XP_310377.7
Saccharomyces cerevisiae (baker's yeast)	RPL30	NP_011485.1
Eremothecium gossypii	AGOS_AFR478W	NP_986025.1
Pan troglodytes (chimpanzee)	RPL30	XP_003951358.1
Drosophila melanogaster (fruit fly)	RpL30	NP_524687.1
Caenorhabditis elegans (roundworm)	rpl-30	NP_492728.2
Kluyveromyces lactis	KLLA0E10847g	XP_454439.1
Schizosaccharomyces pombe (fission yeast)	rpl3001	NP_593558.1
Arabidopsis thaliana (thale cress)	AT3G18740	NP_188504.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural snapshots of actively translating human ribosomes.
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, B?rger J, Mielke T, Scheerer P, Spahn CM
Cell161(4)845-57(2015 May)

Ribosomal protein s15 phosphorylation mediates LRRK2 neurodegeneration in Parkinson's disease.
Martin I, Kim JW, Lee BD, Kang HC, Xu JC, Jia H, Stankowski J, Kim MS, Zhong J, Kumar M, Andrabi SA, Xiong Y, Dickson DW, Wszolek ZK, Pandey A, Dawson TM, Dawson VL
Cell157(2)472-485(2014 Apr)

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Zanivan S, Maione F, Hein MY, Hern?ndez-Fernaud JR, Ostasiewicz P, Giraudo E, Mann M
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)

PHF6 interacts with the nucleosome remodeling and deacetylation (NuRD) complex.
Todd MA, Picketts DJ
Journal of proteome research11(8)4326-37(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

crystal of L30e diffracted to 1.6 A degrees resolution
Title: Crystallization and preliminary X-ray structure analysis of human ribosomal protein L30e.

Knockdown of L29 or L30 enhanced the interaction of MDM2 with L11 and L5 and markedly inhibited MDM2-mediated p53 ubiquitination, suggesting that direct perturbation of 60 S ribosomal biogenesis activates p53 via L11- and L5-mediated MDM2 suppression.
Title: Perturbation of 60 S ribosomal biogenesis results in ribosomal protein L5- and L11-dependent p53 activation.

Gain of 8q-mapped RPL30 gene is associated with adverse outcome in Medulloblastoma.
Title: Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8.

The following RPL30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPL30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02554	NM_000989.4 Latest version!	Homo sapiens ribosomal protein L30 (RPL30), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu02554	NM_000989.3	Homo sapiens ribosomal protein L30 (RPL30), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPL30 ( NM_000989.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000980.1 Homo sapiens ribosomal protein L30 (RPL30), mRNA.

Price & Availability↑

CloneID	OHu02554
Clone ID Related Accession (Same CDS sequence)	NM_000989.4 , NM_000989.3
Accession Version	NM_000989.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	60S ribosomal protein L30
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BM785413.1 and BC095426.1. On Nov 23, 2018 this sequence version replaced NM_000989.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AV761808.1, BE439872.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000287038.8/ ENSP00000287038.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGGCCG CAAAGAAGAC GAAAAAGTCG CTGGAGTCGA TCAACTCTAG GCTCCAACTC 
GTTATGAAAA GTGGGAAGTA CGTCCTGGGG TACAAGCAGA CTCTGAAGAT GATCAGACAA 
GGCAAAGCGA AATTGGTCAT TCTCGCTAAC AACTGCCCAG CTTTGAGGAA ATCTGAAATA 
GAGTACTATG CTATGTTGGC TAAAACTGGT GTCCATCACT ACAGTGGCAA TAATATTGAA 
CTGGGCACAG CATGCGGAAA ATACTACAGA GTGTGCACAC TGGCTATCAT TGATCCAGGT 
GACTCTGACA TCATTAGAAG CATGCCAGAA CAGACTGGTG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000980.1
CDS	71..418
Translation	MVAAKKTKKSLESINSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALRKSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDPGDSDIIRSMPEQTGEK

Target ORF information:

RefSeq Version	NM_000989.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L30 (RPL30), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000989.4

ORF Insert Sequence:

ATGGTGGCCG CAAAGAAGAC GAAAAAGTCG CTGGAGTCGA TCAACTCTAG GCTCCAACTC 
GTTATGAAAA GTGGGAAGTA CGTCCTGGGG TACAAGCAGA CTCTGAAGAT GATCAGACAA 
GGCAAAGCGA AATTGGTCAT TCTCGCTAAC AACTGCCCAG CTTTGAGGAA ATCTGAAATA 
GAGTACTATG CTATGTTGGC TAAAACTGGT GTCCATCACT ACAGTGGCAA TAATATTGAA 
CTGGGCACAG CATGCGGAAA ATACTACAGA GTGTGCACAC TGGCTATCAT TGATCCAGGT 
GACTCTGACA TCATTAGAAG CATGCCAGAA CAGACTGGTG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL30 ( NM_000989.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000980.1 Homo sapiens ribosomal protein L30 (RPL30), mRNA.

Price & Availability↑

CloneID	OHu02554
Clone ID Related Accession (Same CDS sequence)	NM_000989.4 , NM_000989.3
Accession Version	NM_000989.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	60S ribosomal protein L30
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BM785413.1, BC095426.1 and BQ055546.1. On Feb 24, 2011 this sequence version replaced NM_000989.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM785413.1, SRR1163655.362689.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGGCCG CAAAGAAGAC GAAAAAGTCG CTGGAGTCGA TCAACTCTAG GCTCCAACTC 
GTTATGAAAA GTGGGAAGTA CGTCCTGGGG TACAAGCAGA CTCTGAAGAT GATCAGACAA 
GGCAAAGCGA AATTGGTCAT TCTCGCTAAC AACTGCCCAG CTTTGAGGAA ATCTGAAATA 
GAGTACTATG CTATGTTGGC TAAAACTGGT GTCCATCACT ACAGTGGCAA TAATATTGAA 
CTGGGCACAG CATGCGGAAA ATACTACAGA GTGTGCACAC TGGCTATCAT TGATCCAGGT 
GACTCTGACA TCATTAGAAG CATGCCAGAA CAGACTGGTG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000980.1
CDS	116..463
Translation	MVAAKKTKKSLESINSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALRKSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDPGDSDIIRSMPEQTGEK

Target ORF information:

RefSeq Version	NM_000989.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L30 (RPL30), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000989.3

ORF Insert Sequence:

ATGGTGGCCG CAAAGAAGAC GAAAAAGTCG CTGGAGTCGA TCAACTCTAG GCTCCAACTC 
GTTATGAAAA GTGGGAAGTA CGTCCTGGGG TACAAGCAGA CTCTGAAGAT GATCAGACAA 
GGCAAAGCGA AATTGGTCAT TCTCGCTAAC AACTGCCCAG CTTTGAGGAA ATCTGAAATA 
GAGTACTATG CTATGTTGGC TAAAACTGGT GTCCATCACT ACAGTGGCAA TAATATTGAA 
CTGGGCACAG CATGCGGAAA ATACTACAGA GTGTGCACAC TGGCTATCAT TGATCCAGGT 
GACTCTGACA TCATTAGAAG CATGCCAGAA CAGACTGGTG AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural snapshots of actively translating human ribosomes.
Cell161(4)845-57(2015 May)
Behrmann E,Loerke J,Budkevich TV,Yamamoto K,Schmidt A,Penczek PA,Vos MR,B?rger J,Mielke T,Scheerer P,Spahn CM

Ribosomal protein s15 phosphorylation mediates LRRK2 neurodegeneration in Parkinson's disease.
Cell157(2)472-485(2014 Apr)
Martin I,Kim JW,Lee BD,Kang HC,Xu JC,Jia H,Stankowski J,Kim MS,Zhong J,Kumar M,Andrabi SA,Xiong Y,Dickson DW,Wszolek ZK,Pandey A,Dawson TM,Dawson VL

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)
Zanivan S,Maione F,Hein MY,Hern?ndez-Fernaud JR,Ostasiewicz P,Giraudo E,Mann M

PHF6 interacts with the nucleosome remodeling and deacetylation (NuRD) complex.
Journal of proteome research11(8)4326-37(2012 Aug)
Todd MA,Picketts DJ

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RPL30 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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