RPL18 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPL18
Entrez Gene ID	6141
Full Name	ribosomal protein L18
Synonyms	L18
General protein information	Preferred Names ribosomal protein L18 Names 60S ribosomal protein L18 large ribosomal subunit protein eL18
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.33
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18E family of ribosomal proteins that is a component of the 60S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012].
Disorder MIM:	604179

mRNA and Protein(s)

mRNA	Protein	Name
NM_000979.4	NP_000970.1	60S ribosomal protein L18 isoform 1
NM_000979.3	NP_000970.1	60S ribosomal protein L18 isoform 1
NM_001270490.2	NP_001257419.1	60S ribosomal protein L18 isoform 2
NM_001270490.1	NP_001257419.1	60S ribosomal protein L18 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RPL18	XP_003316544.1
Bos taurus (cattle)	RPL18	NP_001015556.1
Drosophila melanogaster (fruit fly)	RpL18	NP_001261501.1
Caenorhabditis elegans (roundworm)	rpl-18	NP_502655.1
Saccharomyces cerevisiae (baker's yeast)	RPL18A	NP_014521.1
Schizosaccharomyces pombe (fission yeast)	rpl1802	NP_001018228.1
Oryza sativa (rice)	Os03g0341100	NP_001050069.1
Xenopus tropicalis (tropical clawed frog)	rpl18	NP_001011030.1
Canis lupus familiaris (dog)	RPL18	NP_001238887.1
Mus musculus (house mouse)	Rpl18	NP_033103.2
Anopheles gambiae (African malaria mosquito)	RL18_ANOGA	XP_308294.3
Saccharomyces cerevisiae (baker's yeast)	RPL18B	NP_014098.1
Kluyveromyces lactis	KLLA0A07227g	XP_451321.1
Magnaporthe oryzae (rice blast fungus)	MGG_04484	XP_003710973.1
Arabidopsis thaliana (thale cress)	AT5G27850	NP_198137.1
Homo sapiens (human)	RPL18	NP_000970.1
	LOC697499	XP_001082813.1
Arabidopsis thaliana (thale cress)	RPL18	NP_187210.1
Rattus norvegicus (Norway rat)	Rpl18	NP_112364.1
Danio rerio (zebrafish)	rpl18	NP_001003432.1
Schizosaccharomyces pombe (fission yeast)	rpl1801	NP_596397.1
Neurospora crassa	NCU03988	XP_956573.1
	LOC704394	XP_001092745.1
Eremothecium gossypii	AGOS_ADL157C	NP_983939.1
Oryza sativa (rice)	Os07g0674700	NP_001060617.1
Oryza sativa (rice)	Os05g0155100	NP_001054688.1

Gene Ontology
Bibliography

Related articles in PubMed

Dengue virus NS1 protein interacts with the ribosomal protein RPL18: this interaction is required for viral translation and replication in Huh-7 cells.
Cervantes-Salazar M, Angel-Ambrocio AH, Soto-Acosta R, Bautista-Carbajal P, Hurtado-Monzon AM, Alcaraz-Estrada SL, Ludert JE, Del Angel RM
Virology484113-26(2015 Oct)

Structural snapshots of actively translating human ribosomes.
Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, B?rger J, Mielke T, Scheerer P, Spahn CM
Cell161(4)845-57(2015 May)

Genetic variations affecting serum carcinoembryonic antigen levels and status of regional lymph nodes in patients with sporadic colorectal cancer from Southern China.
Liang Y, Tang W, Huang T, Gao Y, Tan A, Yang X, Zhang H, Hu Y, Qin X, Li S, Zhang S, Mo L, Liang Z, Shi D, Huang Z, Guan Y, Zhou J, Winkler C, O'Brien SJ, Xu J, Mo Z, Peng T
PloS one9(6)e97923(2014)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Silencing of the RPL-18 does not affect cell translation efficiency or viability, but it reduces significantly viral translation, replication and viral yield, suggesting that the RPL-18 is required during the Dengue virus replicative cycle.
Title: Dengue virus NS1 protein interacts with the ribosomal protein RPL18: this interaction is required for viral translation and replication in Huh-7 cells.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following RPL18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPL18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02550	NM_000979.4 Latest version!	Homo sapiens ribosomal protein L18 (RPL18), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu03680	NM_001270490.2 Latest version!	Homo sapiens ribosomal protein L18 (RPL18), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu02550	NM_000979.3	Homo sapiens ribosomal protein L18 (RPL18), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu03680	NM_001270490.1	Homo sapiens ribosomal protein L18 (RPL18), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPL18 ( NM_000979.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000970.1 Homo sapiens ribosomal protein L18 (RPL18), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02550
Clone ID Related Accession (Same CDS sequence)	NM_000979.4 , NM_000979.3
Accession Version	NM_000979.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	60S ribosomal protein L18 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BM764512.1 and AK026558.1. On Nov 22, 2018 this sequence version replaced NM_000979.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.247865.1, SRR1163657.51232.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000549920.6/ ENSP00000447001.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAGTGG ACATCCGCCA TAACAAGGAC CGAAAGGTTC GGCGCAAGGA GCCCAAGAGC 
CAGGATATCT ACCTGAGGCT GTTGGTCAAG TTATACAGGT TTCTGGCCAG AAGAACCAAC 
TCCACATTCA ACCAGGTTGT GTTGAAGAGG TTGTTTATGA GTCGCACCAA CCGGCCGCCT 
CTGTCCCTTT CCCGGATGAT CCGGAAGATG AAGCTTCCTG GCCGGGAAAA CAAGACGGCC 
GTGGTTGTGG GGACCATAAC TGATGATGTG CGGGTTCAGG AGGTACCCAA ACTGAAGGTA 
TGTGCACTGC GCGTGACCAG CCGGGCCCGC AGCCGCATCC TCAGGGCAGG GGGCAAGATC 
CTCACTTTCG ACCAGCTGGC CCTGGACTCC CCTAAGGGCT GTGGCACTGT CCTGCTCTCC 
GGTCCTCGCA AGGGCCGAGA GGTGTACCGG CATTTCGGCA AGGCCCCAGG AACCCCGCAC 
AGCCACACCA AACCCTACGT CCGCTCCAAG GGCCGGAAGT TCGAGCGTGC CAGAGGCCGA 
CGGGCCAGCC GAGGCTACAA AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000970.1
CDS	36..602
Translation	MGVDIRHNKDRKVRRKEPKSQDIYLRLLVKLYRFLARRTNSTFNQVVLKRLFMSRTNRPPLSLSRMIRKMKLPGRENKTAVVVGTITDDVRVQEVPKLKVCALRVTSRARSRILRAGGKILTFDQLALDSPKGCGTVLLSGPRKGREVYRHFGKAPGTPHSHTKPYVRSKGRKFERARGRRASRGYKN

Target ORF information:

RefSeq Version	NM_000979.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L18 (RPL18), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000979.4

ORF Insert Sequence:

ATGGGAGTGG ACATCCGCCA TAACAAGGAC CGAAAGGTTC GGCGCAAGGA GCCCAAGAGC 
CAGGATATCT ACCTGAGGCT GTTGGTCAAG TTATACAGGT TTCTGGCCAG AAGAACCAAC 
TCCACATTCA ACCAGGTTGT GTTGAAGAGG TTGTTTATGA GTCGCACCAA CCGGCCGCCT 
CTGTCCCTTT CCCGGATGAT CCGGAAGATG AAGCTTCCTG GCCGGGAAAA CAAGACGGCC 
GTGGTTGTGG GGACCATAAC TGATGATGTG CGGGTTCAGG AGGTACCCAA ACTGAAGGTA 
TGTGCACTGC GCGTGACCAG CCGGGCCCGC AGCCGCATCC TCAGGGCAGG GGGCAAGATC 
CTCACTTTCG ACCAGCTGGC CCTGGACTCC CCTAAGGGCT GTGGCACTGT CCTGCTCTCC 
GGTCCTCGCA AGGGCCGAGA GGTGTACCGG CATTTCGGCA AGGCCCCAGG AACCCCGCAC 
AGCCACACCA AACCCTACGT CCGCTCCAAG GGCCGGAAGT TCGAGCGTGC CAGAGGCCGA 
CGGGCCAGCC GAGGCTACAA AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL18 ( NM_000979.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000970.1 Homo sapiens ribosomal protein L18 (RPL18), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02550
Clone ID Related Accession (Same CDS sequence)	NM_000979.4 , NM_000979.3
Accession Version	NM_000979.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	60S ribosomal protein L18 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BM764512.1, AK026558.1 and AI921565.1. On Jul 11, 2012 this sequence version replaced NM_000979.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AI921565.1, SRR1163657.121638.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGAGTGG ACATCCGCCA TAACAAGGAC CGAAAGGTTC GGCGCAAGGA GCCCAAGAGC 
CAGGATATCT ACCTGAGGCT GTTGGTCAAG TTATACAGGT TTCTGGCCAG AAGAACCAAC 
TCCACATTCA ACCAGGTTGT GTTGAAGAGG TTGTTTATGA GTCGCACCAA CCGGCCGCCT 
CTGTCCCTTT CCCGGATGAT CCGGAAGATG AAGCTTCCTG GCCGGGAAAA CAAGACGGCC 
GTGGTTGTGG GGACCATAAC TGATGATGTG CGGGTTCAGG AGGTACCCAA ACTGAAGGTA 
TGTGCACTGC GCGTGACCAG CCGGGCCCGC AGCCGCATCC TCAGGGCAGG GGGCAAGATC 
CTCACTTTCG ACCAGCTGGC CCTGGACTCC CCTAAGGGCT GTGGCACTGT CCTGCTCTCC 
GGTCCTCGCA AGGGCCGAGA GGTGTACCGG CATTTCGGCA AGGCCCCAGG AACCCCGCAC 
AGCCACACCA AACCCTACGT CCGCTCCAAG GGCCGGAAGT TCGAGCGTGC CAGAGGCCGA 
CGGGCCAGCC GAGGCTACAA AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000970.1
CDS	276..842
Translation	MGVDIRHNKDRKVRRKEPKSQDIYLRLLVKLYRFLARRTNSTFNQVVLKRLFMSRTNRPPLSLSRMIRKMKLPGRENKTAVVVGTITDDVRVQEVPKLKVCALRVTSRARSRILRAGGKILTFDQLALDSPKGCGTVLLSGPRKGREVYRHFGKAPGTPHSHTKPYVRSKGRKFERARGRRASRGYKN

Target ORF information:

RefSeq Version	NM_000979.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L18 (RPL18), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000979.3

ORF Insert Sequence:

ATGGGAGTGG ACATCCGCCA TAACAAGGAC CGAAAGGTTC GGCGCAAGGA GCCCAAGAGC 
CAGGATATCT ACCTGAGGCT GTTGGTCAAG TTATACAGGT TTCTGGCCAG AAGAACCAAC 
TCCACATTCA ACCAGGTTGT GTTGAAGAGG TTGTTTATGA GTCGCACCAA CCGGCCGCCT 
CTGTCCCTTT CCCGGATGAT CCGGAAGATG AAGCTTCCTG GCCGGGAAAA CAAGACGGCC 
GTGGTTGTGG GGACCATAAC TGATGATGTG CGGGTTCAGG AGGTACCCAA ACTGAAGGTA 
TGTGCACTGC GCGTGACCAG CCGGGCCCGC AGCCGCATCC TCAGGGCAGG GGGCAAGATC 
CTCACTTTCG ACCAGCTGGC CCTGGACTCC CCTAAGGGCT GTGGCACTGT CCTGCTCTCC 
GGTCCTCGCA AGGGCCGAGA GGTGTACCGG CATTTCGGCA AGGCCCCAGG AACCCCGCAC 
AGCCACACCA AACCCTACGT CCGCTCCAAG GGCCGGAAGT TCGAGCGTGC CAGAGGCCGA 
CGGGCCAGCC GAGGCTACAA AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL18 ( NM_001270490.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257419.1 Homo sapiens ribosomal protein L18 (RPL18), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03680
Clone ID Related Accession (Same CDS sequence)	NM_001270490.2 , NM_001270490.1
Accession Version	NM_001270490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	60S ribosomal protein L18 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD246847.1 and AI921565.1. On May 31, 2019 this sequence version replaced NM_001270490.1. Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD246847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTTATACA GGTTTCTGGC CAGAAGAACC AACTCCACAT TCAACCAGGT TGTGTTGAAG 
AGGTTGTTTA TGAGTCGCAC CAACCGGCCG CCTCTGTCCC TTTCCCGGAT GATCCGGAAG 
ATGAAGCTTC CTGGCCGGGA AAACAAGACG GCCGTGGTTG TGGGGACCAT AACTGATGAT 
GTGCGGGTTC AGGAGGTACC CAAACTGAAG GTATGTGCAC TGCGCGTGAC CAGCCGGGCC 
CGCAGCCGCA TCCTCAGGGC AGGGGGCAAG ATCCTCACTT TCGACCAGCT GGCCCTGGAC 
TCCCCTAAGG GCTGTGGCAC TGTCCTGCTC TCCGGTCCTC GCAAGGGCCG AGAGGTGTAC 
CGGCATTTCG GCAAGGCCCC AGGAACCCCG CACAGCCACA CCAAACCCTA CGTCCGCTCC 
AAGGGCCGGA AGTTCGAGCG TGCCAGAGGC CGACGGGCCA GCCGAGGCTA CAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257419.1
CDS	36..515
Translation	MLYRFLARRTNSTFNQVVLKRLFMSRTNRPPLSLSRMIRKMKLPGRENKTAVVVGTITDDVRVQEVPKLKVCALRVTSRARSRILRAGGKILTFDQLALDSPKGCGTVLLSGPRKGREVYRHFGKAPGTPHSHTKPYVRSKGRKFERARGRRASRGYKN

Target ORF information:

RefSeq Version	NM_001270490.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L18 (RPL18), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270490.2

ORF Insert Sequence:

ATGTTATACA GGTTTCTGGC CAGAAGAACC AACTCCACAT TCAACCAGGT TGTGTTGAAG 
AGGTTGTTTA TGAGTCGCAC CAACCGGCCG CCTCTGTCCC TTTCCCGGAT GATCCGGAAG 
ATGAAGCTTC CTGGCCGGGA AAACAAGACG GCCGTGGTTG TGGGGACCAT AACTGATGAT 
GTGCGGGTTC AGGAGGTACC CAAACTGAAG GTATGTGCAC TGCGCGTGAC CAGCCGGGCC 
CGCAGCCGCA TCCTCAGGGC AGGGGGCAAG ATCCTCACTT TCGACCAGCT GGCCCTGGAC 
TCCCCTAAGG GCTGTGGCAC TGTCCTGCTC TCCGGTCCTC GCAAGGGCCG AGAGGTGTAC 
CGGCATTTCG GCAAGGCCCC AGGAACCCCG CACAGCCACA CCAAACCCTA CGTCCGCTCC 
AAGGGCCGGA AGTTCGAGCG TGCCAGAGGC CGACGGGCCA GCCGAGGCTA CAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL18 ( NM_001270490.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257419.1 Homo sapiens ribosomal protein L18 (RPL18), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03680
Clone ID Related Accession (Same CDS sequence)	NM_001270490.2 , NM_001270490.1
Accession Version	NM_001270490.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-10
Organism	Homo sapiens(human)
Product	60S ribosomal protein L18 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM764512.1, CD246847.1 and AI921565.1. Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD246847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTTATACA GGTTTCTGGC CAGAAGAACC AACTCCACAT TCAACCAGGT TGTGTTGAAG 
AGGTTGTTTA TGAGTCGCAC CAACCGGCCG CCTCTGTCCC TTTCCCGGAT GATCCGGAAG 
ATGAAGCTTC CTGGCCGGGA AAACAAGACG GCCGTGGTTG TGGGGACCAT AACTGATGAT 
GTGCGGGTTC AGGAGGTACC CAAACTGAAG GTATGTGCAC TGCGCGTGAC CAGCCGGGCC 
CGCAGCCGCA TCCTCAGGGC AGGGGGCAAG ATCCTCACTT TCGACCAGCT GGCCCTGGAC 
TCCCCTAAGG GCTGTGGCAC TGTCCTGCTC TCCGGTCCTC GCAAGGGCCG AGAGGTGTAC 
CGGCATTTCG GCAAGGCCCC AGGAACCCCG CACAGCCACA CCAAACCCTA CGTCCGCTCC 
AAGGGCCGGA AGTTCGAGCG TGCCAGAGGC CGACGGGCCA GCCGAGGCTA CAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257419.1
CDS	276..755
Translation	MLYRFLARRTNSTFNQVVLKRLFMSRTNRPPLSLSRMIRKMKLPGRENKTAVVVGTITDDVRVQEVPKLKVCALRVTSRARSRILRAGGKILTFDQLALDSPKGCGTVLLSGPRKGREVYRHFGKAPGTPHSHTKPYVRSKGRKFERARGRRASRGYKN

Target ORF information:

RefSeq Version	NM_001270490.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L18 (RPL18), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270490.1

ORF Insert Sequence:

ATGTTATACA GGTTTCTGGC CAGAAGAACC AACTCCACAT TCAACCAGGT TGTGTTGAAG 
AGGTTGTTTA TGAGTCGCAC CAACCGGCCG CCTCTGTCCC TTTCCCGGAT GATCCGGAAG 
ATGAAGCTTC CTGGCCGGGA AAACAAGACG GCCGTGGTTG TGGGGACCAT AACTGATGAT 
GTGCGGGTTC AGGAGGTACC CAAACTGAAG GTATGTGCAC TGCGCGTGAC CAGCCGGGCC 
CGCAGCCGCA TCCTCAGGGC AGGGGGCAAG ATCCTCACTT TCGACCAGCT GGCCCTGGAC 
TCCCCTAAGG GCTGTGGCAC TGTCCTGCTC TCCGGTCCTC GCAAGGGCCG AGAGGTGTAC 
CGGCATTTCG GCAAGGCCCC AGGAACCCCG CACAGCCACA CCAAACCCTA CGTCCGCTCC 
AAGGGCCGGA AGTTCGAGCG TGCCAGAGGC CGACGGGCCA GCCGAGGCTA CAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dengue virus NS1 protein interacts with the ribosomal protein RPL18: this interaction is required for viral translation and replication in Huh-7 cells.
Virology484113-26(2015 Oct)
Cervantes-Salazar M,Angel-Ambrocio AH,Soto-Acosta R,Bautista-Carbajal P,Hurtado-Monzon AM,Alcaraz-Estrada SL,Ludert JE,Del Angel RM

Structural snapshots of actively translating human ribosomes.
Cell161(4)845-57(2015 May)
Behrmann E,Loerke J,Budkevich TV,Yamamoto K,Schmidt A,Penczek PA,Vos MR,B?rger J,Mielke T,Scheerer P,Spahn CM

Genetic variations affecting serum carcinoembryonic antigen levels and status of regional lymph nodes in patients with sporadic colorectal cancer from Southern China.
PloS one9(6)e97923(2014)
Liang Y,Tang W,Huang T,Gao Y,Tan A,Yang X,Zhang H,Hu Y,Qin X,Li S,Zhang S,Mo L,Liang Z,Shi D,Huang Z,Guan Y,Zhou J,Winkler C,O'Brien SJ,Xu J,Mo Z,Peng T

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

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RPL18 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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