RPL7A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPL7A
Entrez Gene ID	6130
Full Name	ribosomal protein L7a
Synonyms	L7A,SURF3,TRUP
General protein information	Preferred Names ribosomal protein L7a Names 60S ribosomal protein L7a PLA-X polypeptide large ribosomal subunit protein eL8 surfeit 3 surfeit locus protein 3 thyroid hormone receptor uncoupling protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.2
Summary	Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008].
Disorder MIM:	185640

mRNA and Protein(s)

mRNA	Protein	Name
NM_000972.3	NP_000963.1	60S ribosomal protein L7a
NM_000972.2	NP_000963.1	60S ribosomal protein L7a

Pathways from BioSystems

Homologies

	RPL7A	NP_001137511.1
Bos taurus (cattle)	RPL7A	NP_001035610.1
Kluyveromyces lactis	KLLA0E00573g	XP_002999386.1
Canis lupus familiaris (dog)	RPL7A	XP_537800.1
Gallus gallus (chicken)	RPL7A	NP_001004379.1
Schizosaccharomyces pombe (fission yeast)	rpl8	NP_595832.1
Pan troglodytes (chimpanzee)	RPL7A	NP_001233532.1
Mus musculus (house mouse)	Rpl7a	NP_038749.1
Eremothecium gossypii	AGOS_ACL070C	NP_983334.1
Magnaporthe oryzae (rice blast fungus)	MGG_04612	XP_003713624.1
Rattus norvegicus (Norway rat)	Rpl7a	NP_001107863.1
Danio rerio (zebrafish)	rpl7a	NP_956341.1
Saccharomyces cerevisiae (baker's yeast)	RPL8B	NP_013055.1
Neurospora crassa	NCU04779	XP_960228.1
Homo sapiens (human)	RPL7A	NP_000963.1
Saccharomyces cerevisiae (baker's yeast)	RPL8A	NP_011830.1

Gene Ontology
Bibliography

Related articles in PubMed

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP
Journal of virology88(18)10340-53(2014 Sep)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

X-ray diffraction data of RPL7a were collected to a resolution of 3.5 A from a crystal belonging to the tetragonal space group P4(1)22 or P4(3)22 with unit-cell parameters a = 92.28, b = 92.28, c = 236.59 A
Title: Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of human ribosomal protein L7a (RPL7a).

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

Under-expression of RPL7A may be involved in the carcinogenesis of osteosarcoma
Title: Down-regulation of ribosomal protein L7A in human osteosarcoma.

the L7a protein contains two RNA-binding domains: one encompassing aa 52-100 (RNAB1) and the other encompassing aa 101-161 (RNAB2)
Title: Ribosomal protein L7a binds RNA through two distinct RNA-binding domains.

The following RPL7A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPL7A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31068	NM_000972.3 Latest version!	Homo sapiens ribosomal protein L7a (RPL7A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu31068	NM_000972.2	Homo sapiens ribosomal protein L7a (RPL7A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPL7A ( NM_000972.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000963.1 Homo sapiens ribosomal protein L7a (RPL7A), mRNA.

Price & Availability↑

CloneID	OHu31068
Clone ID Related Accession (Same CDS sequence)	NM_000972.3 , NM_000972.2
Accession Version	NM_000972.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	60S ribosomal protein L7a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AL772161.10 and AL593848.15. On Nov 26, 2018 this sequence version replaced NM_000972.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.152640.1, SRR5189655.22992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000323345.11/ ENSP00000361076.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGAAAG GAAAGAAGGC CAAGGGAAAG AAGGTGGCTC CGGCCCCAGC TGTCGTGAAG 
AAGCAGGAGG CTAAGAAAGT GGTGAATCCC CTGTTTGAGA AAAGGCCTAA GAATTTTGGC 
ATTGGACAGG ACATCCAGCC CAAAAGAGAC CTCACCCGCT TTGTGAAATG GCCCCGCTAT 
ATCAGGTTGC AGCGGCAGAG AGCCATCCTC TATAAGCGGC TGAAAGTGCC TCCTGCGATT 
AACCAGTTCA CCCAGGCCCT GGACCGCCAA ACAGCTACTC AGCTGCTTAA GCTGGCCCAC 
AAGTACAGAC CAGAGACAAA GCAAGAGAAG AAGCAGAGAC TGTTGGCCCG GGCCGAGAAG 
AAGGCTGCTG GCAAAGGGGA CGTCCCAACG AAGAGACCAC CTGTCCTTCG AGCAGGAGTT 
AACACCGTCA CCACCTTGGT GGAGAACAAG AAAGCTCAGC TGGTGGTGAT TGCACACGAC 
GTGGATCCCA TCGAGCTGGT TGTCTTCTTG CCTGCCCTGT GTCGTAAAAT GGGGGTCCCT 
TACTGCATTA TCAAGGGAAA GGCAAGACTG GGACGTCTAG TCCACAGGAA GACCTGCACC 
ACTGTCGCCT TCACACAGGT GAACTCGGAA GACAAAGGCG CTTTGGCTAA GCTGGTGGAA 
GCTATCAGGA CCAATTACAA TGACAGATAC GATGAGATCC GCCGTCACTG GGGTGGCAAT 
GTCCTGGGTC CTAAGTCTGT GGCTCGTATC GCCAAGCTCG AAAAGGCAAA GGCTAAAGAA 
CTTGCCACTA AACTGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000963.1
CDS	27..827
Translation	MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG

Target ORF information:

RefSeq Version	NM_000972.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L7a (RPL7A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000972.3

ORF Insert Sequence:

ATGCCGAAAG GAAAGAAGGC CAAGGGAAAG AAGGTGGCTC CGGCCCCAGC TGTCGTGAAG 
AAGCAGGAGG CTAAGAAAGT GGTGAATCCC CTGTTTGAGA AAAGGCCTAA GAATTTTGGC 
ATTGGACAGG ACATCCAGCC CAAAAGAGAC CTCACCCGCT TTGTGAAATG GCCCCGCTAT 
ATCAGGTTGC AGCGGCAGAG AGCCATCCTC TATAAGCGGC TGAAAGTGCC TCCTGCGATT 
AACCAGTTCA CCCAGGCCCT GGACCGCCAA ACAGCTACTC AGCTGCTTAA GCTGGCCCAC 
AAGTACAGAC CAGAGACAAA GCAAGAGAAG AAGCAGAGAC TGTTGGCCCG GGCCGAGAAG 
AAGGCTGCTG GCAAAGGGGA CGTCCCAACG AAGAGACCAC CTGTCCTTCG AGCAGGAGTT 
AACACCGTCA CCACCTTGGT GGAGAACAAG AAAGCTCAGC TGGTGGTGAT TGCACACGAC 
GTGGATCCCA TCGAGCTGGT TGTCTTCTTG CCTGCCCTGT GTCGTAAAAT GGGGGTCCCT 
TACTGCATTA TCAAGGGAAA GGCAAGACTG GGACGTCTAG TCCACAGGAA GACCTGCACC 
ACTGTCGCCT TCACACAGGT GAACTCGGAA GACAAAGGCG CTTTGGCTAA GCTGGTGGAA 
GCTATCAGGA CCAATTACAA TGACAGATAC GATGAGATCC GCCGTCACTG GGGTGGCAAT 
GTCCTGGGTC CTAAGTCTGT GGCTCGTATC GCCAAGCTCG AAAAGGCAAA GGCTAAAGAA 
CTTGCCACTA AACTGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL7A ( NM_000972.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000963.1 Homo sapiens ribosomal protein L7a (RPL7A), mRNA.

Price & Availability↑

CloneID	OHu31068
Clone ID Related Accession (Same CDS sequence)	NM_000972.3 , NM_000972.2
Accession Version	NM_000972.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	60S ribosomal protein L7a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from M36072.1. On Jan 29, 2002 this sequence version replaced NM_000972.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.376577.1, SRR1163655.613060.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCGAAAG GAAAGAAGGC CAAGGGAAAG AAGGTGGCTC CGGCCCCAGC TGTCGTGAAG 
AAGCAGGAGG CTAAGAAAGT GGTGAATCCC CTGTTTGAGA AAAGGCCTAA GAATTTTGGC 
ATTGGACAGG ACATCCAGCC CAAAAGAGAC CTCACCCGCT TTGTGAAATG GCCCCGCTAT 
ATCAGGTTGC AGCGGCAGAG AGCCATCCTC TATAAGCGGC TGAAAGTGCC TCCTGCGATT 
AACCAGTTCA CCCAGGCCCT GGACCGCCAA ACAGCTACTC AGCTGCTTAA GCTGGCCCAC 
AAGTACAGAC CAGAGACAAA GCAAGAGAAG AAGCAGAGAC TGTTGGCCCG GGCCGAGAAG 
AAGGCTGCTG GCAAAGGGGA CGTCCCAACG AAGAGACCAC CTGTCCTTCG AGCAGGAGTT 
AACACCGTCA CCACCTTGGT GGAGAACAAG AAAGCTCAGC TGGTGGTGAT TGCACACGAC 
GTGGATCCCA TCGAGCTGGT TGTCTTCTTG CCTGCCCTGT GTCGTAAAAT GGGGGTCCCT 
TACTGCATTA TCAAGGGAAA GGCAAGACTG GGACGTCTAG TCCACAGGAA GACCTGCACC 
ACTGTCGCCT TCACACAGGT GAACTCGGAA GACAAAGGCG CTTTGGCTAA GCTGGTGGAA 
GCTATCAGGA CCAATTACAA TGACAGATAC GATGAGATCC GCCGTCACTG GGGTGGCAAT 
GTCCTGGGTC CTAAGTCTGT GGCTCGTATC GCCAAGCTCG AAAAGGCAAA GGCTAAAGAA 
CTTGCCACTA AACTGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000963.1
CDS	31..831
Translation	MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG

Target ORF information:

RefSeq Version	NM_000972.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L7a (RPL7A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000972.2

ORF Insert Sequence:

ATGCCGAAAG GAAAGAAGGC CAAGGGAAAG AAGGTGGCTC CGGCCCCAGC TGTCGTGAAG 
AAGCAGGAGG CTAAGAAAGT GGTGAATCCC CTGTTTGAGA AAAGGCCTAA GAATTTTGGC 
ATTGGACAGG ACATCCAGCC CAAAAGAGAC CTCACCCGCT TTGTGAAATG GCCCCGCTAT 
ATCAGGTTGC AGCGGCAGAG AGCCATCCTC TATAAGCGGC TGAAAGTGCC TCCTGCGATT 
AACCAGTTCA CCCAGGCCCT GGACCGCCAA ACAGCTACTC AGCTGCTTAA GCTGGCCCAC 
AAGTACAGAC CAGAGACAAA GCAAGAGAAG AAGCAGAGAC TGTTGGCCCG GGCCGAGAAG 
AAGGCTGCTG GCAAAGGGGA CGTCCCAACG AAGAGACCAC CTGTCCTTCG AGCAGGAGTT 
AACACCGTCA CCACCTTGGT GGAGAACAAG AAAGCTCAGC TGGTGGTGAT TGCACACGAC 
GTGGATCCCA TCGAGCTGGT TGTCTTCTTG CCTGCCCTGT GTCGTAAAAT GGGGGTCCCT 
TACTGCATTA TCAAGGGAAA GGCAAGACTG GGACGTCTAG TCCACAGGAA GACCTGCACC 
ACTGTCGCCT TCACACAGGT GAACTCGGAA GACAAAGGCG CTTTGGCTAA GCTGGTGGAA 
GCTATCAGGA CCAATTACAA TGACAGATAC GATGAGATCC GCCGTCACTG GGGTGGCAAT 
GTCCTGGGTC CTAAGTCTGT GGCTCGTATC GCCAAGCTCG AAAAGGCAAA GGCTAAAGAA 
CTTGCCACTA AACTGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Journal of virology88(18)10340-53(2014 Sep)
Jefferson M,Donaszi-Ivanov A,Pollen S,Dalmay T,Saalbach G,Powell PP

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

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RPL7A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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