RPL5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPL5
Entrez Gene ID	6125
Full Name	ribosomal protein L5
Synonyms	L5,MSTP030,PPP1R135
General protein information	Preferred Names ribosomal protein L5 Names 60S ribosomal protein L5 large ribosomal subunit protein uL18 protein phosphatase 1, regulatory subunit 135
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p22.1
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18P family of ribosomal proteins and component of the 60S subunit. The encoded protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The encoded protein may also function to inhibit tumorigenesis through the activation of downstream tumor suppressors and the downregulation of oncoprotein expression. Mutations in this gene have been identified in patients with Diamond-Blackfan Anemia (DBA). This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. [provided by RefSeq, Mar 2017].
Disorder MIM:	603634
Disorder Html:	Diamond-Blackfan anemia 6, 612561 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000969.5	NP_000960.2	60S ribosomal protein L5
NM_000969.3	NP_000960.2	60S ribosomal protein L5
NM_000969.4	NP_000960.2	60S ribosomal protein L5

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	RL5_ANOGA	XP_319782.3
Schizosaccharomyces pombe (fission yeast)	rpl502	NP_596399.1
Arabidopsis thaliana (thale cress)	ATL5	NP_566767.1
Homo sapiens (human)	RPL5	NP_000960.2
	LOC705979	XP_001100058.1
Caenorhabditis elegans (roundworm)	rpl-5	NP_495811.1
Eremothecium gossypii	AGOS_ACR104C	NP_983506.1
Neurospora crassa	NCU04331	XP_960578.1
Drosophila melanogaster (fruit fly)	RpL5	NP_001036390.2
Kluyveromyces lactis	KLLA0D06941g	XP_453370.1
Pan troglodytes (chimpanzee)	RPL5	XP_003308262.1
Danio rerio (zebrafish)	rpl5a	NP_956050.1
Danio rerio (zebrafish)	rpl5b	NP_001002106.1
Schizosaccharomyces pombe (fission yeast)	rpl501	NP_594180.1
Magnaporthe oryzae (rice blast fungus)	MGG_07048	XP_003715227.1
Canis lupus familiaris (dog)	RPL5	XP_537074.4
Bos taurus (cattle)	RPL5	NP_001030383.1
Mus musculus (house mouse)	Rpl5	NP_058676.1
Rattus norvegicus (Norway rat)	Rpl5	NP_112361.1
Gallus gallus (chicken)	RPL5	NP_989912.1
Saccharomyces cerevisiae (baker's yeast)	RPL5	NP_015194.1
Arabidopsis thaliana (thale cress)	RPL5B	NP_198790.1
Oryza sativa (rice)	Os01g0896800	NP_001045083.1
Xenopus tropicalis (tropical clawed frog)	rpl5	NP_988881.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular analysis and genotype-phenotype correlation of Diamond-Blackfan anemia.
Arbiv OA, Cuvelier G, Klaassen RJ, Fernandez CV, Robitaille N, Steele M, Breakey V, Abish S, Wu J, Sinha R, Silva M, Goodyear L, Jardine L, Lipton JH, Corriveau-Bourque C, Brossard J, Michon B, Ghemlas I, Waespe N, Zlateska B, Sung L, Cada M, Dror Y
Clinical genetics(2017 Oct)

RPL5 on 1p22.1 is recurrently deleted in multiple myeloma and its expression is linked to bortezomib response.
Hofman IJF, van Duin M, De Bruyne E, Fancello L, Mulligan G, Geerdens E, Garelli E, Mancini C, Lemmens H, Delforge M, Vandenberghe P, Wlodarska I, Aspesi A, Michaux L, Vanderkerken K, Sonneveld P, De Keersmaecker K
Leukemia31(8)1706-1714(2017 Aug)

Low frequency mutations in ribosomal proteins RPL10 and RPL5 in multiple myeloma.
Hofman IJF, Patchett S, van Duin M, Geerdens E, Verbeeck J, Michaux L, Delforge M, Sonneveld P, Johnson AW, De Keersmaecker K
Haematologica102(8)e317-e320(2017 Aug)

Whole exome sequencing in the differential diagnosis of Diamond-Blackfan anemia: Clinical and molecular study of three patients with novel RPL5 and mosaic RPS19 mutations.
Errichiello E, Vetro A, Mina T, Wischmeijer A, Berrino E, Carella M, Romagnoli M, Sacchini P, Venesio T, Zecca M, Zuffardi O
Blood cells, molecules & diseases6438-44(2017 May)

The ribosomal protein gene RPL5 is a haploinsufficient tumor suppressor in multiple cancer types.
Fancello L, Kampen KR, Hofman IJ, Verbeeck J, De Keersmaecker K
Oncotarget8(9)14462-14478(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Low RPL5 expression is associated with cancer.
Title: The ribosomal protein gene RPL5 is a haploinsufficient tumor suppressor in multiple cancer types.

Ribosomal proteins L11 and L5 activate TAp73 by overcoming MDM2 inhibition.
Title: Ribosomal proteins L11 and L5 activate TAp73 by overcoming MDM2 inhibition.

RPL5 mutation is associated with Diamond Blackfan Anemia.
Title: Clinical phenotype and genetic analysis of RPS19, RPL5, and RPL11 genes in Greek patients with Diamond Blackfan Anemia.

Findings uncover a mechanism by which RPL5 and RPL11 can co-operatively suppress c-Myc expression, allowing a tightly controlled ribosome biogenesis in cells.
Title: Ribosomal proteins L5 and L11 co-operatively inactivate c-Myc via RNA-induced silencing complex.

Unlike other tumor suppressors, RPL5 and RPL11 play essential roles in normal cell proliferation.
Title: Loss of tumor suppressor RPL5/RPL11 does not induce cell cycle arrest but impedes proliferation due to reduced ribosome content and translation capacity.

The following RPL5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPL5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28222	NM_000969.5 Latest version!	Homo sapiens ribosomal protein L5 (RPL5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu28222	NM_000969.3	Homo sapiens ribosomal protein L5 (RPL5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu28222	NM_000969.4	Homo sapiens ribosomal protein L5 (RPL5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPL5 ( NM_000969.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000960.2 Homo sapiens ribosomal protein L5 (RPL5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28222
Clone ID Related Accession (Same CDS sequence)	NM_000969.5 , NM_000969.3 , NM_000969.4
Accession Version	NM_000969.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	60S ribosomal protein L5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BP247508.1, AF113210.1 and N67963.1. This sequence is a reference standard in the RefSeqGene project. On May 9, 2018 this sequence version replaced NM_000969.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the supported protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1139019.1, SRR3476690.127437.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000370321.8/ ENSP00000359345.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGTTTG TTAAAGTTGT TAAGAATAAG GCCTACTTTA AGAGATACCA AGTGAAATTT 
AGAAGACGAC GAGAGGGTAA AACTGATTAT TATGCTCGGA AACGCTTGGT GATACAAGAT 
AAAAATAAAT ACAACACACC CAAATACAGG ATGATAGTTC GTGTGACAAA CAGAGATATC 
ATTTGTCAGA TTGCTTATGC CCGTATAGAG GGGGATATGA TAGTCTGCGC AGCGTATGCA 
CACGAACTGC CAAAATATGG TGTGAAGGTT GGCCTGACAA ATTATGCTGC AGCATATTGT 
ACTGGCCTGC TGCTGGCCCG CAGGCTTCTC AATAGGTTTG GCATGGACAA GATCTATGAA 
GGCCAAGTGG AGGTGACTGG TGATGAATAC AATGTGGAAA GCATTGATGG TCAGCCAGGT 
GCCTTCACCT GCTATTTGGA TGCAGGCCTT GCCAGAACTA CCACTGGCAA TAAAGTTTTT 
GGTGCCCTGA AGGGAGCTGT GGATGGAGGC TTGTCTATCC CTCACAGTAC CAAACGATTC 
CCTGGTTATG ATTCTGAAAG CAAGGAATTT AATGCAGAAG TACATCGGAA GCACATCATG 
GGCCAGAATG TTGCAGATTA CATGCGCTAC TTAATGGAAG AAGATGAAGA TGCTTACAAG 
AAACAGTTCT CTCAATACAT AAAGAACAGC GTAACTCCAG ACATGATGGA GGAGATGTAT 
AAGAAAGCTC ATGCTGCTAT ACGAGAGAAT CCAGTCTATG AAAAGAAGCC CAAGAAAGAA 
GTTAAAAAGA AGAGGTGGAA CCGTCCCAAA ATGTCCCTTG CTCAGAAGAA GGATCGGGTA 
GCTCAAAAGA AGGCAAGCTT CCTCAGAGCT CAGGAGCGGG CTGCTGAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000960.2
CDS	76..969
Translation	MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES

Target ORF information:

RefSeq Version	NM_000969.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L5 (RPL5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000969.5

ORF Insert Sequence:

ATGGGGTTTG TTAAAGTTGT TAAGAATAAG GCCTACTTTA AGAGATACCA AGTGAAATTT 
AGAAGACGAC GAGAGGGTAA AACTGATTAT TATGCTCGGA AACGCTTGGT GATACAAGAT 
AAAAATAAAT ACAACACACC CAAATACAGG ATGATAGTTC GTGTGACAAA CAGAGATATC 
ATTTGTCAGA TTGCTTATGC CCGTATAGAG GGGGATATGA TAGTCTGCGC AGCGTATGCA 
CACGAACTGC CAAAATATGG TGTGAAGGTT GGCCTGACAA ATTATGCTGC AGCATATTGT 
ACTGGCCTGC TGCTGGCCCG CAGGCTTCTC AATAGGTTTG GCATGGACAA GATCTATGAA 
GGCCAAGTGG AGGTGACTGG TGATGAATAC AATGTGGAAA GCATTGATGG TCAGCCAGGT 
GCCTTCACCT GCTATTTGGA TGCAGGCCTT GCCAGAACTA CCACTGGCAA TAAAGTTTTT 
GGTGCCCTGA AGGGAGCTGT GGATGGAGGC TTGTCTATCC CTCACAGTAC CAAACGATTC 
CCTGGTTATG ATTCTGAAAG CAAGGAATTT AATGCAGAAG TACATCGGAA GCACATCATG 
GGCCAGAATG TTGCAGATTA CATGCGCTAC TTAATGGAAG AAGATGAAGA TGCTTACAAG 
AAACAGTTCT CTCAATACAT AAAGAACAGC GTAACTCCAG ACATGATGGA GGAGATGTAT 
AAGAAAGCTC ATGCTGCTAT ACGAGAGAAT CCAGTCTATG AAAAGAAGCC CAAGAAAGAA 
GTTAAAAAGA AGAGGTGGAA CCGTCCCAAA ATGTCCCTTG CTCAGAAGAA GGATCGGGTA 
GCTCAAAAGA AGGCAAGCTT CCTCAGAGCT CAGGAGCGGG CTGCTGAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL5 ( NM_000969.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000960.2 Homo sapiens ribosomal protein L5 (RPL5), mRNA.

Price & Availability↑

CloneID	OHu28222
Clone ID Related Accession (Same CDS sequence)	NM_000969.5 , NM_000969.3 , NM_000969.4
Accession Version	NM_000969.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	60S ribosomal protein L5
Comment

ATGGGGTTTG TTAAAGTTGT TAAGAATAAG GCCTACTTTA AGAGATACCA AGTGAAATTT 
AGAAGACGAC GAGAGGGTAA AACTGATTAT TATGCTCGGA AACGCTTGGT GATACAAGAT 
AAAAATAAAT ACAACACACC CAAATACAGG ATGATAGTTC GTGTGACAAA CAGAGATATC 
ATTTGTCAGA TTGCTTATGC CCGTATAGAG GGGGATATGA TAGTCTGCGC AGCGTATGCA 
CACGAACTGC CAAAATATGG TGTGAAGGTT GGCCTGACAA ATTATGCTGC AGCATATTGT 
ACTGGCCTGC TGCTGGCCCG CAGGCTTCTC AATAGGTTTG GCATGGACAA GATCTATGAA 
GGCCAAGTGG AGGTGACTGG TGATGAATAC AATGTGGAAA GCATTGATGG TCAGCCAGGT 
GCCTTCACCT GCTATTTGGA TGCAGGCCTT GCCAGAACTA CCACTGGCAA TAAAGTTTTT 
GGTGCCCTGA AGGGAGCTGT GGATGGAGGC TTGTCTATCC CTCACAGTAC CAAACGATTC 
CCTGGTTATG ATTCTGAAAG CAAGGAATTT AATGCAGAAG TACATCGGAA GCACATCATG 
GGCCAGAATG TTGCAGATTA CATGCGCTAC TTAATGGAAG AAGATGAAGA TGCTTACAAG 
AAACAGTTCT CTCAATACAT AAAGAACAGC GTAACTCCAG ACATGATGGA GGAGATGTAT 
AAGAAAGCTC ATGCTGCTAT ACGAGAGAAT CCAGTCTATG AAAAGAAGCC CAAGAAAGAA 
GTTAAAAAGA AGAGGTGGAA CCGTCCCAAA ATGTCCCTTG CTCAGAAGAA GGATCGGGTA 
GCTCAAAAGA AGGCAAGCTT CCTCAGAGCT CAGGAGCGGG CTGCTGAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000960.2
Translation	MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES

Target ORF information:

RefSeq Version	NM_000969.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens ribosomal protein L5 (RPL5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000969.3

ORF Insert Sequence:

ATGGGGTTTG TTAAAGTTGT TAAGAATAAG GCCTACTTTA AGAGATACCA AGTGAAATTT 
AGAAGACGAC GAGAGGGTAA AACTGATTAT TATGCTCGGA AACGCTTGGT GATACAAGAT 
AAAAATAAAT ACAACACACC CAAATACAGG ATGATAGTTC GTGTGACAAA CAGAGATATC 
ATTTGTCAGA TTGCTTATGC CCGTATAGAG GGGGATATGA TAGTCTGCGC AGCGTATGCA 
CACGAACTGC CAAAATATGG TGTGAAGGTT GGCCTGACAA ATTATGCTGC AGCATATTGT 
ACTGGCCTGC TGCTGGCCCG CAGGCTTCTC AATAGGTTTG GCATGGACAA GATCTATGAA 
GGCCAAGTGG AGGTGACTGG TGATGAATAC AATGTGGAAA GCATTGATGG TCAGCCAGGT 
GCCTTCACCT GCTATTTGGA TGCAGGCCTT GCCAGAACTA CCACTGGCAA TAAAGTTTTT 
GGTGCCCTGA AGGGAGCTGT GGATGGAGGC TTGTCTATCC CTCACAGTAC CAAACGATTC 
CCTGGTTATG ATTCTGAAAG CAAGGAATTT AATGCAGAAG TACATCGGAA GCACATCATG 
GGCCAGAATG TTGCAGATTA CATGCGCTAC TTAATGGAAG AAGATGAAGA TGCTTACAAG 
AAACAGTTCT CTCAATACAT AAAGAACAGC GTAACTCCAG ACATGATGGA GGAGATGTAT 
AAGAAAGCTC ATGCTGCTAT ACGAGAGAAT CCAGTCTATG AAAAGAAGCC CAAGAAAGAA 
GTTAAAAAGA AGAGGTGGAA CCGTCCCAAA ATGTCCCTTG CTCAGAAGAA GGATCGGGTA 
GCTCAAAAGA AGGCAAGCTT CCTCAGAGCT CAGGAGCGGG CTGCTGAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL5 ( NM_000969.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000960.2 Homo sapiens ribosomal protein L5 (RPL5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28222
Clone ID Related Accession (Same CDS sequence)	NM_000969.5 , NM_000969.3 , NM_000969.4
Accession Version	NM_000969.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-15
Organism	Homo sapiens(human)
Product	60S ribosomal protein L5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AL162740.13, BP247508.1, AF113210.1 and N67963.1. This sequence is a reference standard in the RefSeqGene project. On Mar 30, 2017 this sequence version replaced NM_000969.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.552130.1, SRR1163657.485435.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGTTTG TTAAAGTTGT TAAGAATAAG GCCTACTTTA AGAGATACCA AGTGAAATTT 
AGAAGACGAC GAGAGGGTAA AACTGATTAT TATGCTCGGA AACGCTTGGT GATACAAGAT 
AAAAATAAAT ACAACACACC CAAATACAGG ATGATAGTTC GTGTGACAAA CAGAGATATC 
ATTTGTCAGA TTGCTTATGC CCGTATAGAG GGGGATATGA TAGTCTGCGC AGCGTATGCA 
CACGAACTGC CAAAATATGG TGTGAAGGTT GGCCTGACAA ATTATGCTGC AGCATATTGT 
ACTGGCCTGC TGCTGGCCCG CAGGCTTCTC AATAGGTTTG GCATGGACAA GATCTATGAA 
GGCCAAGTGG AGGTGACTGG TGATGAATAC AATGTGGAAA GCATTGATGG TCAGCCAGGT 
GCCTTCACCT GCTATTTGGA TGCAGGCCTT GCCAGAACTA CCACTGGCAA TAAAGTTTTT 
GGTGCCCTGA AGGGAGCTGT GGATGGAGGC TTGTCTATCC CTCACAGTAC CAAACGATTC 
CCTGGTTATG ATTCTGAAAG CAAGGAATTT AATGCAGAAG TACATCGGAA GCACATCATG 
GGCCAGAATG TTGCAGATTA CATGCGCTAC TTAATGGAAG AAGATGAAGA TGCTTACAAG 
AAACAGTTCT CTCAATACAT AAAGAACAGC GTAACTCCAG ACATGATGGA GGAGATGTAT 
AAGAAAGCTC ATGCTGCTAT ACGAGAGAAT CCAGTCTATG AAAAGAAGCC CAAGAAAGAA 
GTTAAAAAGA AGAGGTGGAA CCGTCCCAAA ATGTCCCTTG CTCAGAAGAA GGATCGGGTA 
GCTCAAAAGA AGGCAAGCTT CCTCAGAGCT CAGGAGCGGG CTGCTGAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000960.2
CDS	130..1023
Translation	MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES

Target ORF information:

RefSeq Version	NM_000969.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L5 (RPL5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000969.4

ORF Insert Sequence:

ATGGGGTTTG TTAAAGTTGT TAAGAATAAG GCCTACTTTA AGAGATACCA AGTGAAATTT 
AGAAGACGAC GAGAGGGTAA AACTGATTAT TATGCTCGGA AACGCTTGGT GATACAAGAT 
AAAAATAAAT ACAACACACC CAAATACAGG ATGATAGTTC GTGTGACAAA CAGAGATATC 
ATTTGTCAGA TTGCTTATGC CCGTATAGAG GGGGATATGA TAGTCTGCGC AGCGTATGCA 
CACGAACTGC CAAAATATGG TGTGAAGGTT GGCCTGACAA ATTATGCTGC AGCATATTGT 
ACTGGCCTGC TGCTGGCCCG CAGGCTTCTC AATAGGTTTG GCATGGACAA GATCTATGAA 
GGCCAAGTGG AGGTGACTGG TGATGAATAC AATGTGGAAA GCATTGATGG TCAGCCAGGT 
GCCTTCACCT GCTATTTGGA TGCAGGCCTT GCCAGAACTA CCACTGGCAA TAAAGTTTTT 
GGTGCCCTGA AGGGAGCTGT GGATGGAGGC TTGTCTATCC CTCACAGTAC CAAACGATTC 
CCTGGTTATG ATTCTGAAAG CAAGGAATTT AATGCAGAAG TACATCGGAA GCACATCATG 
GGCCAGAATG TTGCAGATTA CATGCGCTAC TTAATGGAAG AAGATGAAGA TGCTTACAAG 
AAACAGTTCT CTCAATACAT AAAGAACAGC GTAACTCCAG ACATGATGGA GGAGATGTAT 
AAGAAAGCTC ATGCTGCTAT ACGAGAGAAT CCAGTCTATG AAAAGAAGCC CAAGAAAGAA 
GTTAAAAAGA AGAGGTGGAA CCGTCCCAAA ATGTCCCTTG CTCAGAAGAA GGATCGGGTA 
GCTCAAAAGA AGGCAAGCTT CCTCAGAGCT CAGGAGCGGG CTGCTGAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular analysis and genotype-phenotype correlation of Diamond-Blackfan anemia.
Clinical genetics(2017 Oct)
Arbiv OA,Cuvelier G,Klaassen RJ,Fernandez CV,Robitaille N,Steele M,Breakey V,Abish S,Wu J,Sinha R,Silva M,Goodyear L,Jardine L,Lipton JH,Corriveau-Bourque C,Brossard J,Michon B,Ghemlas I,Waespe N,Zlateska B,Sung L,Cada M,Dror Y

RPL5 on 1p22.1 is recurrently deleted in multiple myeloma and its expression is linked to bortezomib response.
Leukemia31(8)1706-1714(2017 Aug)
Hofman IJF,van Duin M,De Bruyne E,Fancello L,Mulligan G,Geerdens E,Garelli E,Mancini C,Lemmens H,Delforge M,Vandenberghe P,Wlodarska I,Aspesi A,Michaux L,Vanderkerken K,Sonneveld P,De Keersmaecker K

Low frequency mutations in ribosomal proteins RPL10 and RPL5 in multiple myeloma.
Haematologica102(8)e317-e320(2017 Aug)
Hofman IJF,Patchett S,van Duin M,Geerdens E,Verbeeck J,Michaux L,Delforge M,Sonneveld P,Johnson AW,De Keersmaecker K

Whole exome sequencing in the differential diagnosis of Diamond-Blackfan anemia: Clinical and molecular study of three patients with novel RPL5 and mosaic RPS19 mutations.
Blood cells, molecules & diseases6438-44(2017 May)
Errichiello E,Vetro A,Mina T,Wischmeijer A,Berrino E,Carella M,Romagnoli M,Sacchini P,Venesio T,Zecca M,Zuffardi O

The ribosomal protein gene RPL5 is a haploinsufficient tumor suppressor in multiple cancer types.
Oncotarget8(9)14462-14478(2017 Feb)
Fancello L,Kampen KR,Hofman IJ,Verbeeck J,De Keersmaecker K

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RPL5 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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