RPL3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPL3
Entrez Gene ID	6122
Full Name	ribosomal protein L3
Synonyms	ASC-1,L3,TARBP-B
General protein information	Preferred Names ribosomal protein L3 Names 60S ribosomal protein L3 HIV-1 TAR RNA-binding protein B large ribosomal subunit protein uL3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.1
Summary	Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008].
Disorder MIM:	604163

mRNA and Protein(s)

mRNA	Protein	Name
NM_000967.3	NP_000958.1	60S ribosomal protein L3 isoform a
NM_000967.4	NP_000958.1	60S ribosomal protein L3 isoform a
NM_001033853.2	NP_001029025.1	60S ribosomal protein L3 isoform b
NM_001033853.1	NP_001029025.1	60S ribosomal protein L3 isoform b

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RPL3	NP_777140.1
Mus musculus (house mouse)	Rpl3	NP_038790.2
Saccharomyces cerevisiae (baker's yeast)	RPL3	NP_014706.1
Schizosaccharomyces pombe (fission yeast)	rpl302	NP_594780.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003556	XP_313303.5
Eremothecium gossypii	AGOS_ADR206W	NP_984302.2
Homo sapiens (human)	RPL3	NP_000958.1
Danio rerio (zebrafish)	rpl3	NP_001001590.1
Drosophila melanogaster (fruit fly)	RpL3	NP_731548.2
Kluyveromyces lactis	KLLA0F16511g	XP_455822.1
Pan troglodytes (chimpanzee)	RPL3	XP_525601.3
Caenorhabditis elegans (roundworm)	rpl-3	NP_001021254.1
Schizosaccharomyces pombe (fission yeast)	rpl301	NP_593471.1
Neurospora crassa	NCU06843	XP_963317.2
Arabidopsis thaliana (thale cress)	RPL3B	NP_176352.1
Xenopus tropicalis (tropical clawed frog)	rpl3	NP_001120075.1
	LOC693573	XP_001082799.2
Canis lupus familiaris (dog)	RPL3	NP_001239088.1
Rattus norvegicus (Norway rat)	Rpl3	NP_942048.1
Gallus gallus (chicken)	RPL3	NP_001006241.1
Magnaporthe oryzae (rice blast fungus)	MGG_03193	XP_003716797.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of uL3 in Multidrug Resistance in p53-Mutated Lung Cancer Cells.
Russo A, Saide A, Smaldone S, Faraonio R, Russo G
International journal of molecular sciences18(3)(2017 Mar)

Enhancement of 5-FU sensitivity by the proapoptotic rpL3 gene in p53 null colon cancer cells through combined polymer nanoparticles.
Russo A, Maiolino S, Pagliara V, Ungaro F, Tatangelo F, Leone A, Scalia G, Budillon A, Quaglia F, Russo G
Oncotarget7(48)79670-79687(2016 Nov)

5-FU targets rpL3 to induce mitochondrial apoptosis via cystathionine-?-synthase in colon cancer cells lacking p53.
Pagliara V, Saide A, Mitidieri E, d'Emmanuele di Villa Bianca R, Sorrentino R, Russo G, Russo A
Oncotarget7(31)50333-50348(2016 Aug)

The DHX33 RNA Helicase Promotes mRNA Translation Initiation.
Zhang Y, You J, Wang X, Weber J
Molecular and cellular biology35(17)2918-31(2015 Sep)

Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein.
Li Y, Jain N, Limpanawat S, To J, Quistgaard EM, Nordlund P, Thanabalu T, Torres J
Virology482105-10(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that uL3 down-regulation correlates positively with multidrug resistance. Restoration of the uL3 protein level re-sensitized the resistant cells to the drug by regulating the reactive oxygen species (ROS) levels, glutathione content, glutamate release, and cystine uptake.
Title: Role of uL3 in Multidrug Resistance in p53-Mutated Lung Cancer Cells.

rpL3 participates in the cell response to chemotherapy acting as a critical regulator of cell cycle, apoptosis and DNA repair, by modulating p21 expression
Title: Human rpL3 plays a crucial role in cell response to nucleolar stress induced by 5-FU and L-OHP.

The rpL3 is able to modulate the protein amounts of p21 through a novel pathway independent from p53 involving Sp1 and NPM.
Title: Human rpL3 induces G?/S arrest or apoptosis by modulating p21 (waf1/cip1) levels in a p53-independent manner.

NPM and KHSRP are two newly identified proteins involved in the regulation of rpL3 gene expression via alternative pre-mRNA splicing.
Title: Autoregulatory circuit of human rpL3 expression requires hnRNP H1, NPM and KHSRP.

working model in which rpL3 recruits hnRNP H1 and, through cooperation with other splicing factors, promotes selection of the alternative splice site
Title: hnRNP H1 and intronic G runs in the splicing control of the human rpL3 gene.

The following RPL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02542	NM_000967.4 Latest version!	Homo sapiens ribosomal protein L3 (RPL3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu04586	NM_001033853.2 Latest version!	Homo sapiens ribosomal protein L3 (RPL3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu02542	NM_000967.3	Homo sapiens ribosomal protein L3 (RPL3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu04586	NM_001033853.1	Homo sapiens ribosomal protein L3 (RPL3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPL3 ( NM_000967.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000958.1 Homo sapiens ribosomal protein L3 (RPL3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02542
Clone ID Related Accession (Same CDS sequence)	NM_000967.3 , NM_000967.4
Accession Version	NM_000967.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	60S ribosomal protein L3 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BU584440.1, AK130450.1, BC012146.1 and BC071666.1. On Sep 28, 2005 this sequence version replaced NM_000967.2. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071666.1, ERR279869.3313.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTCACA GAAAGTTCTC CGCTCCCAGA CATGGGTCCC TCGGCTTCCT GCCTCGGAAG 
CGCAGCAGCA GGCATCGTGG GAAGGTGAAG AGCTTCCCTA AGGATGACCC GTCCAAGCCG 
GTCCACCTCA CAGCCTTCCT GGGATACAAG GCTGGCATGA CTCACATCGT GCGGGAAGTC 
GACAGGCCGG GATCCAAGGT GAACAAGAAG GAGGTGGTGG AGGCTGTGAC CATTGTAGAG 
ACACCACCCA TGGTGGTTGT GGGCATTGTG GGCTACGTGG AAACCCCTCG AGGCCTCCGG 
ACCTTCAAGA CTGTCTTTGC TGAGCACATC AGTGATGAAT GCAAGAGGCG TTTCTATAAG 
AATTGGCATA AATCTAAGAA GAAGGCCTTT ACCAAGTACT GCAAGAAATG GCAGGATGAG 
GATGGCAAGA AGCAGCTGGA GAAGGACTTC AGCAGCATGA AGAAGTACTG CCAAGTCATC 
CGTGTCATTG CCCACACCCA GATGCGCCTG CTTCCTCTGC GCCAGAAGAA GGCCCACCTG 
ATGGAGATCC AGGTGAACGG AGGCACTGTG GCCGAGAAGC TGGACTGGGC CCGCGAGAGG 
CTTGAGCAGC AGGTACCTGT GAACCAAGTG TTTGGGCAGG ATGAGATGAT CGACGTCATC 
GGGGTGACCA AGGGCAAAGG CTACAAAGGG GTCACCAGTC GTTGGCACAC CAAGAAGCTG 
CCCCGCAAGA CCCACCGAGG CCTGCGCAAG GTGGCCTGTA TTGGGGCATG GCATCCTGCT 
CGTGTAGCCT TCTCTGTGGC ACGCGCTGGG CAGAAAGGCT ACCATCACCG CACTGAGATC 
AACAAGAAGA TTTATAAGAT TGGCCAGGGC TACCTTATCA AGGACGGCAA GCTGATCAAG 
AACAATGCCT CCACTGACTA TGACCTATCT GACAAGAGCA TCAACCCTCT GGGTGGCTTT 
GTCCACTATG GTGAAGTGAC CAATGACTTT GTCATGCTGA AAGGCTGTGT GGTGGGAACC 
AAGAAGCGGG TGCTCACCCT CCGCAAGTCC TTGCTGGTGC AGACGAAGCG GCGGGCTCTG 
GAGAAGATTG ACCTTAAGTT CATTGACACC ACCTCCAAGT TTGGCCATGG CCGCTTCCAG 
ACCATGGAGG AGAAGAAAGC ATTCATGGGA CCACTGAAGA AAGACCGAAT TGCAAAGGAA 
GAAGGAGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000958.1
CDS	69..1280
Translation	MSHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDPSKPVHLTAFLGYKAGMTHIVREVDRPGSKVNKKEVVEAVTIVETPPMVVVGIVGYVETPRGLRTFKTVFAEHISDECKRRFYKNWHKSKKKAFTKYCKKWQDEDGKKQLEKDFSSMKKYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIKNNASTDYDLSDKSINPLGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTMEEKKAFMGPLKKDRIAKEEGA

Target ORF information:

RefSeq Version	NM_000967.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L3 (RPL3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000967.3

ORF Insert Sequence:

ATGTCTCACA GAAAGTTCTC CGCTCCCAGA CATGGGTCCC TCGGCTTCCT GCCTCGGAAG 
CGCAGCAGCA GGCATCGTGG GAAGGTGAAG AGCTTCCCTA AGGATGACCC GTCCAAGCCG 
GTCCACCTCA CAGCCTTCCT GGGATACAAG GCTGGCATGA CTCACATCGT GCGGGAAGTC 
GACAGGCCGG GATCCAAGGT GAACAAGAAG GAGGTGGTGG AGGCTGTGAC CATTGTAGAG 
ACACCACCCA TGGTGGTTGT GGGCATTGTG GGCTACGTGG AAACCCCTCG AGGCCTCCGG 
ACCTTCAAGA CTGTCTTTGC TGAGCACATC AGTGATGAAT GCAAGAGGCG TTTCTATAAG 
AATTGGCATA AATCTAAGAA GAAGGCCTTT ACCAAGTACT GCAAGAAATG GCAGGATGAG 
GATGGCAAGA AGCAGCTGGA GAAGGACTTC AGCAGCATGA AGAAGTACTG CCAAGTCATC 
CGTGTCATTG CCCACACCCA GATGCGCCTG CTTCCTCTGC GCCAGAAGAA GGCCCACCTG 
ATGGAGATCC AGGTGAACGG AGGCACTGTG GCCGAGAAGC TGGACTGGGC CCGCGAGAGG 
CTTGAGCAGC AGGTACCTGT GAACCAAGTG TTTGGGCAGG ATGAGATGAT CGACGTCATC 
GGGGTGACCA AGGGCAAAGG CTACAAAGGG GTCACCAGTC GTTGGCACAC CAAGAAGCTG 
CCCCGCAAGA CCCACCGAGG CCTGCGCAAG GTGGCCTGTA TTGGGGCATG GCATCCTGCT 
CGTGTAGCCT TCTCTGTGGC ACGCGCTGGG CAGAAAGGCT ACCATCACCG CACTGAGATC 
AACAAGAAGA TTTATAAGAT TGGCCAGGGC TACCTTATCA AGGACGGCAA GCTGATCAAG 
AACAATGCCT CCACTGACTA TGACCTATCT GACAAGAGCA TCAACCCTCT GGGTGGCTTT 
GTCCACTATG GTGAAGTGAC CAATGACTTT GTCATGCTGA AAGGCTGTGT GGTGGGAACC 
AAGAAGCGGG TGCTCACCCT CCGCAAGTCC TTGCTGGTGC AGACGAAGCG GCGGGCTCTG 
GAGAAGATTG ACCTTAAGTT CATTGACACC ACCTCCAAGT TTGGCCATGG CCGCTTCCAG 
ACCATGGAGG AGAAGAAAGC ATTCATGGGA CCACTGAAGA AAGACCGAAT TGCAAAGGAA 
GAAGGAGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL3 ( NM_000967.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000958.1 Homo sapiens ribosomal protein L3 (RPL3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02542
Clone ID Related Accession (Same CDS sequence)	NM_000967.3 , NM_000967.4
Accession Version	NM_000967.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	60S ribosomal protein L3 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AK130450.1, BC012146.1 and BC071666.1. On Nov 22, 2018 this sequence version replaced NM_000967.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.788490.1, SRR5189652.122410.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216146.9/ ENSP00000346001.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTCACA GAAAGTTCTC CGCTCCCAGA CATGGGTCCC TCGGCTTCCT GCCTCGGAAG 
CGCAGCAGCA GGCATCGTGG GAAGGTGAAG AGCTTCCCTA AGGATGACCC GTCCAAGCCG 
GTCCACCTCA CAGCCTTCCT GGGATACAAG GCTGGCATGA CTCACATCGT GCGGGAAGTC 
GACAGGCCGG GATCCAAGGT GAACAAGAAG GAGGTGGTGG AGGCTGTGAC CATTGTAGAG 
ACACCACCCA TGGTGGTTGT GGGCATTGTG GGCTACGTGG AAACCCCTCG AGGCCTCCGG 
ACCTTCAAGA CTGTCTTTGC TGAGCACATC AGTGATGAAT GCAAGAGGCG TTTCTATAAG 
AATTGGCATA AATCTAAGAA GAAGGCCTTT ACCAAGTACT GCAAGAAATG GCAGGATGAG 
GATGGCAAGA AGCAGCTGGA GAAGGACTTC AGCAGCATGA AGAAGTACTG CCAAGTCATC 
CGTGTCATTG CCCACACCCA GATGCGCCTG CTTCCTCTGC GCCAGAAGAA GGCCCACCTG 
ATGGAGATCC AGGTGAACGG AGGCACTGTG GCCGAGAAGC TGGACTGGGC CCGCGAGAGG 
CTTGAGCAGC AGGTACCTGT GAACCAAGTG TTTGGGCAGG ATGAGATGAT CGACGTCATC 
GGGGTGACCA AGGGCAAAGG CTACAAAGGG GTCACCAGTC GTTGGCACAC CAAGAAGCTG 
CCCCGCAAGA CCCACCGAGG CCTGCGCAAG GTGGCCTGTA TTGGGGCATG GCATCCTGCT 
CGTGTAGCCT TCTCTGTGGC ACGCGCTGGG CAGAAAGGCT ACCATCACCG CACTGAGATC 
AACAAGAAGA TTTATAAGAT TGGCCAGGGC TACCTTATCA AGGACGGCAA GCTGATCAAG 
AACAATGCCT CCACTGACTA TGACCTATCT GACAAGAGCA TCAACCCTCT GGGTGGCTTT 
GTCCACTATG GTGAAGTGAC CAATGACTTT GTCATGCTGA AAGGCTGTGT GGTGGGAACC 
AAGAAGCGGG TGCTCACCCT CCGCAAGTCC TTGCTGGTGC AGACGAAGCG GCGGGCTCTG 
GAGAAGATTG ACCTTAAGTT CATTGACACC ACCTCCAAGT TTGGCCATGG CCGCTTCCAG 
ACCATGGAGG AGAAGAAAGC ATTCATGGGA CCACTGAAGA AAGACCGAAT TGCAAAGGAA 
GAAGGAGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000958.1
CDS	27..1238
Translation	MSHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDPSKPVHLTAFLGYKAGMTHIVREVDRPGSKVNKKEVVEAVTIVETPPMVVVGIVGYVETPRGLRTFKTVFAEHISDECKRRFYKNWHKSKKKAFTKYCKKWQDEDGKKQLEKDFSSMKKYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIKNNASTDYDLSDKSINPLGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTMEEKKAFMGPLKKDRIAKEEGA

Target ORF information:

RefSeq Version	NM_000967.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L3 (RPL3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000967.4

ORF Insert Sequence:

ATGTCTCACA GAAAGTTCTC CGCTCCCAGA CATGGGTCCC TCGGCTTCCT GCCTCGGAAG 
CGCAGCAGCA GGCATCGTGG GAAGGTGAAG AGCTTCCCTA AGGATGACCC GTCCAAGCCG 
GTCCACCTCA CAGCCTTCCT GGGATACAAG GCTGGCATGA CTCACATCGT GCGGGAAGTC 
GACAGGCCGG GATCCAAGGT GAACAAGAAG GAGGTGGTGG AGGCTGTGAC CATTGTAGAG 
ACACCACCCA TGGTGGTTGT GGGCATTGTG GGCTACGTGG AAACCCCTCG AGGCCTCCGG 
ACCTTCAAGA CTGTCTTTGC TGAGCACATC AGTGATGAAT GCAAGAGGCG TTTCTATAAG 
AATTGGCATA AATCTAAGAA GAAGGCCTTT ACCAAGTACT GCAAGAAATG GCAGGATGAG 
GATGGCAAGA AGCAGCTGGA GAAGGACTTC AGCAGCATGA AGAAGTACTG CCAAGTCATC 
CGTGTCATTG CCCACACCCA GATGCGCCTG CTTCCTCTGC GCCAGAAGAA GGCCCACCTG 
ATGGAGATCC AGGTGAACGG AGGCACTGTG GCCGAGAAGC TGGACTGGGC CCGCGAGAGG 
CTTGAGCAGC AGGTACCTGT GAACCAAGTG TTTGGGCAGG ATGAGATGAT CGACGTCATC 
GGGGTGACCA AGGGCAAAGG CTACAAAGGG GTCACCAGTC GTTGGCACAC CAAGAAGCTG 
CCCCGCAAGA CCCACCGAGG CCTGCGCAAG GTGGCCTGTA TTGGGGCATG GCATCCTGCT 
CGTGTAGCCT TCTCTGTGGC ACGCGCTGGG CAGAAAGGCT ACCATCACCG CACTGAGATC 
AACAAGAAGA TTTATAAGAT TGGCCAGGGC TACCTTATCA AGGACGGCAA GCTGATCAAG 
AACAATGCCT CCACTGACTA TGACCTATCT GACAAGAGCA TCAACCCTCT GGGTGGCTTT 
GTCCACTATG GTGAAGTGAC CAATGACTTT GTCATGCTGA AAGGCTGTGT GGTGGGAACC 
AAGAAGCGGG TGCTCACCCT CCGCAAGTCC TTGCTGGTGC AGACGAAGCG GCGGGCTCTG 
GAGAAGATTG ACCTTAAGTT CATTGACACC ACCTCCAAGT TTGGCCATGG CCGCTTCCAG 
ACCATGGAGG AGAAGAAAGC ATTCATGGGA CCACTGAAGA AAGACCGAAT TGCAAAGGAA 
GAAGGAGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL3 ( NM_001033853.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001029025.1 Homo sapiens ribosomal protein L3 (RPL3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04586
Clone ID Related Accession (Same CDS sequence)	NM_001033853.2 , NM_001033853.1
Accession Version	NM_001033853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	60S ribosomal protein L3 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092695.1, BC008492.1 and BC071666.1. On May 31, 2019 this sequence version replaced NM_001033853.1. Transcript Variant: This variant (2) uses alternate in-frame splice sites in two exons, compared to variant 1, resulting in a shorter protein (isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK092695.1, SRR7346977.84346.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTCACA GAAAGTTCTC CGCTCCCAGA CATGGGTCCC TCGGCTTCCT GCCTCGGAAG 
CGCAGCAGCA GGCATCGTGG GAAGGTGAAG AGCTTCCCTA AGGATGACCC GTCCAAGCCG 
GTCCACCTCA CAGCCTTCCT GGGATACAAG GCTGGCATGA CTCACATCGT GCGGGAAGTC 
GACAGGCCGG GATCCAAGGT GAACAAGAAG GAGGTGGTGG AGGCTGTGAC CATTGTAGAG 
ACACCACCCA TGGTGGTTGT GGGCATTGTG GGCTACGTGG AAACCCCTCG AGGCCTCCGG 
ACCTTCAAGA CTGTCTTTGC TGAGCACATC AGTGATGAAT GCAAGAGGCG TTTCTATAAG 
AATTGGCATA AATCTAAGAA GAAGGCCCAC CTGATGGAGA TCCAGGTGAA CGGAGGCACT 
GTGGCCGAGA AGCTGGACTG GGCCCGCGAG AGGCTTGAGC AGCAGGTACC TGTGAACCAA 
GTGTTTGGGC AGGATGAGAT GATCGACGTC ATCGGGGTGA CCAAGGGCAA AGGCTACAAA 
GGGGTCACCA GTCGTTGGCA CACCAAGAAG CTGCCCCGCA AGACCCACCG AGGCCTGCGC 
AAGGTGGCCT GTATTGGGGC ATGGCATCCT GCTCGTGTAG CCTTCTCTGT GGCACGCGCT 
GGGCAGAAAG GCTACCATCA CCGCACTGAG ATCAACAAGA AGATTTATAA GATTGGCCAG 
GGCTACCTTA TCAAGGACGG CAAGCTGATC AAGAACAATG CCTCCACTGA CTATGACCTA 
TCTGACAAGA GCATCAACCC TCTGGGTGGC TTTGTCCACT ATGGTGAAGT GACCAATGAC 
TTTGTCATGC TGAAAGGCTG TGTGGTGGGA ACCAAGAAGC GGGTGCTCAC CCTCCGCAAG 
TCCTTGCTGG TGCAGACGAA GCGGCGGGCT CTGGAGAAGA TTGACCTTAA GTTCATTGAC 
ACCACCTCCA AGTTTGGCCA TGGCCGCTTC CAGACCATGG AGGAGAAGAA AGCATTCATG 
GGACCACTGA AGAAAGACCG AATTGCAAAG GAAGAAGGAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029025.1
CDS	27..1091
Translation	MSHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDPSKPVHLTAFLGYKAGMTHIVREVDRPGSKVNKKEVVEAVTIVETPPMVVVGIVGYVETPRGLRTFKTVFAEHISDECKRRFYKNWHKSKKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIKNNASTDYDLSDKSINPLGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTMEEKKAFMGPLKKDRIAKEEGA

Target ORF information:

RefSeq Version	NM_001033853.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L3 (RPL3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033853.2

ORF Insert Sequence:

ATGTCTCACA GAAAGTTCTC CGCTCCCAGA CATGGGTCCC TCGGCTTCCT GCCTCGGAAG 
CGCAGCAGCA GGCATCGTGG GAAGGTGAAG AGCTTCCCTA AGGATGACCC GTCCAAGCCG 
GTCCACCTCA CAGCCTTCCT GGGATACAAG GCTGGCATGA CTCACATCGT GCGGGAAGTC 
GACAGGCCGG GATCCAAGGT GAACAAGAAG GAGGTGGTGG AGGCTGTGAC CATTGTAGAG 
ACACCACCCA TGGTGGTTGT GGGCATTGTG GGCTACGTGG AAACCCCTCG AGGCCTCCGG 
ACCTTCAAGA CTGTCTTTGC TGAGCACATC AGTGATGAAT GCAAGAGGCG TTTCTATAAG 
AATTGGCATA AATCTAAGAA GAAGGCCCAC CTGATGGAGA TCCAGGTGAA CGGAGGCACT 
GTGGCCGAGA AGCTGGACTG GGCCCGCGAG AGGCTTGAGC AGCAGGTACC TGTGAACCAA 
GTGTTTGGGC AGGATGAGAT GATCGACGTC ATCGGGGTGA CCAAGGGCAA AGGCTACAAA 
GGGGTCACCA GTCGTTGGCA CACCAAGAAG CTGCCCCGCA AGACCCACCG AGGCCTGCGC 
AAGGTGGCCT GTATTGGGGC ATGGCATCCT GCTCGTGTAG CCTTCTCTGT GGCACGCGCT 
GGGCAGAAAG GCTACCATCA CCGCACTGAG ATCAACAAGA AGATTTATAA GATTGGCCAG 
GGCTACCTTA TCAAGGACGG CAAGCTGATC AAGAACAATG CCTCCACTGA CTATGACCTA 
TCTGACAAGA GCATCAACCC TCTGGGTGGC TTTGTCCACT ATGGTGAAGT GACCAATGAC 
TTTGTCATGC TGAAAGGCTG TGTGGTGGGA ACCAAGAAGC GGGTGCTCAC CCTCCGCAAG 
TCCTTGCTGG TGCAGACGAA GCGGCGGGCT CTGGAGAAGA TTGACCTTAA GTTCATTGAC 
ACCACCTCCA AGTTTGGCCA TGGCCGCTTC CAGACCATGG AGGAGAAGAA AGCATTCATG 
GGACCACTGA AGAAAGACCG AATTGCAAAG GAAGAAGGAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL3 ( NM_001033853.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001029025.1 Homo sapiens ribosomal protein L3 (RPL3), transcript variant 2, mRNA.

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CloneID	OHu04586
Clone ID Related Accession (Same CDS sequence)	NM_001033853.2 , NM_001033853.1
Accession Version	NM_001033853.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	60S ribosomal protein L3 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU584440.1, AK092695.1, BC008492.1 and BC071666.1. Transcript Variant: This variant (2) uses alternate in-frame splice sites in two exons, compared to variant 1, resulting in a shorter protein (isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK092695.1, SRR1163657.280657.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTCACA GAAAGTTCTC CGCTCCCAGA CATGGGTCCC TCGGCTTCCT GCCTCGGAAG 
CGCAGCAGCA GGCATCGTGG GAAGGTGAAG AGCTTCCCTA AGGATGACCC GTCCAAGCCG 
GTCCACCTCA CAGCCTTCCT GGGATACAAG GCTGGCATGA CTCACATCGT GCGGGAAGTC 
GACAGGCCGG GATCCAAGGT GAACAAGAAG GAGGTGGTGG AGGCTGTGAC CATTGTAGAG 
ACACCACCCA TGGTGGTTGT GGGCATTGTG GGCTACGTGG AAACCCCTCG AGGCCTCCGG 
ACCTTCAAGA CTGTCTTTGC TGAGCACATC AGTGATGAAT GCAAGAGGCG TTTCTATAAG 
AATTGGCATA AATCTAAGAA GAAGGCCCAC CTGATGGAGA TCCAGGTGAA CGGAGGCACT 
GTGGCCGAGA AGCTGGACTG GGCCCGCGAG AGGCTTGAGC AGCAGGTACC TGTGAACCAA 
GTGTTTGGGC AGGATGAGAT GATCGACGTC ATCGGGGTGA CCAAGGGCAA AGGCTACAAA 
GGGGTCACCA GTCGTTGGCA CACCAAGAAG CTGCCCCGCA AGACCCACCG AGGCCTGCGC 
AAGGTGGCCT GTATTGGGGC ATGGCATCCT GCTCGTGTAG CCTTCTCTGT GGCACGCGCT 
GGGCAGAAAG GCTACCATCA CCGCACTGAG ATCAACAAGA AGATTTATAA GATTGGCCAG 
GGCTACCTTA TCAAGGACGG CAAGCTGATC AAGAACAATG CCTCCACTGA CTATGACCTA 
TCTGACAAGA GCATCAACCC TCTGGGTGGC TTTGTCCACT ATGGTGAAGT GACCAATGAC 
TTTGTCATGC TGAAAGGCTG TGTGGTGGGA ACCAAGAAGC GGGTGCTCAC CCTCCGCAAG 
TCCTTGCTGG TGCAGACGAA GCGGCGGGCT CTGGAGAAGA TTGACCTTAA GTTCATTGAC 
ACCACCTCCA AGTTTGGCCA TGGCCGCTTC CAGACCATGG AGGAGAAGAA AGCATTCATG 
GGACCACTGA AGAAAGACCG AATTGCAAAG GAAGAAGGAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029025.1
CDS	69..1133
Translation	MSHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDPSKPVHLTAFLGYKAGMTHIVREVDRPGSKVNKKEVVEAVTIVETPPMVVVGIVGYVETPRGLRTFKTVFAEHISDECKRRFYKNWHKSKKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIKNNASTDYDLSDKSINPLGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTMEEKKAFMGPLKKDRIAKEEGA

Target ORF information:

RefSeq Version	NM_001033853.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L3 (RPL3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033853.1

ORF Insert Sequence:

ATGTCTCACA GAAAGTTCTC CGCTCCCAGA CATGGGTCCC TCGGCTTCCT GCCTCGGAAG 
CGCAGCAGCA GGCATCGTGG GAAGGTGAAG AGCTTCCCTA AGGATGACCC GTCCAAGCCG 
GTCCACCTCA CAGCCTTCCT GGGATACAAG GCTGGCATGA CTCACATCGT GCGGGAAGTC 
GACAGGCCGG GATCCAAGGT GAACAAGAAG GAGGTGGTGG AGGCTGTGAC CATTGTAGAG 
ACACCACCCA TGGTGGTTGT GGGCATTGTG GGCTACGTGG AAACCCCTCG AGGCCTCCGG 
ACCTTCAAGA CTGTCTTTGC TGAGCACATC AGTGATGAAT GCAAGAGGCG TTTCTATAAG 
AATTGGCATA AATCTAAGAA GAAGGCCCAC CTGATGGAGA TCCAGGTGAA CGGAGGCACT 
GTGGCCGAGA AGCTGGACTG GGCCCGCGAG AGGCTTGAGC AGCAGGTACC TGTGAACCAA 
GTGTTTGGGC AGGATGAGAT GATCGACGTC ATCGGGGTGA CCAAGGGCAA AGGCTACAAA 
GGGGTCACCA GTCGTTGGCA CACCAAGAAG CTGCCCCGCA AGACCCACCG AGGCCTGCGC 
AAGGTGGCCT GTATTGGGGC ATGGCATCCT GCTCGTGTAG CCTTCTCTGT GGCACGCGCT 
GGGCAGAAAG GCTACCATCA CCGCACTGAG ATCAACAAGA AGATTTATAA GATTGGCCAG 
GGCTACCTTA TCAAGGACGG CAAGCTGATC AAGAACAATG CCTCCACTGA CTATGACCTA 
TCTGACAAGA GCATCAACCC TCTGGGTGGC TTTGTCCACT ATGGTGAAGT GACCAATGAC 
TTTGTCATGC TGAAAGGCTG TGTGGTGGGA ACCAAGAAGC GGGTGCTCAC CCTCCGCAAG 
TCCTTGCTGG TGCAGACGAA GCGGCGGGCT CTGGAGAAGA TTGACCTTAA GTTCATTGAC 
ACCACCTCCA AGTTTGGCCA TGGCCGCTTC CAGACCATGG AGGAGAAGAA AGCATTCATG 
GGACCACTGA AGAAAGACCG AATTGCAAAG GAAGAAGGAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of uL3 in Multidrug Resistance in p53-Mutated Lung Cancer Cells.
International journal of molecular sciences18(3)(2017 Mar)
Russo A,Saide A,Smaldone S,Faraonio R,Russo G

Enhancement of 5-FU sensitivity by the proapoptotic rpL3 gene in p53 null colon cancer cells through combined polymer nanoparticles.
Oncotarget7(48)79670-79687(2016 Nov)
Russo A,Maiolino S,Pagliara V,Ungaro F,Tatangelo F,Leone A,Scalia G,Budillon A,Quaglia F,Russo G

5-FU targets rpL3 to induce mitochondrial apoptosis via cystathionine-?-synthase in colon cancer cells lacking p53.
Oncotarget7(31)50333-50348(2016 Aug)
Pagliara V,Saide A,Mitidieri E,d'Emmanuele di Villa Bianca R,Sorrentino R,Russo G,Russo A

The DHX33 RNA Helicase Promotes mRNA Translation Initiation.
Molecular and cellular biology35(17)2918-31(2015 Sep)
Zhang Y,You J,Wang X,Weber J

Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein.
Virology482105-10(2015 Aug)
Li Y,Jain N,Limpanawat S,To J,Quistgaard EM,Nordlund P,Thanabalu T,Torres J

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RPL3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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