RPE65 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPE65
Entrez Gene ID	6121
Full Name	RPE65, retinoid isomerohydrolase
Synonyms	BCO3,LCA2,RP20,mRPE65,p63,rd12,sRPE65
General protein information	Preferred Names RPE65, retinoid isomerohydrolase Names retinoid isomerohydrolase BCO family, member 3 RBP-binding membrane protein all-trans-retinyl-palmitate hydrolase meso-zeaxanthin isomerase retinal pigment epithelium specific protein 65 retinal pigment epithelium-specific 65 kDa protein retinal pigment epithelium-specific protein 65kDa retinitis pigmentosa 20 (autosomal recessive) retinol isomerase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p31.3
Summary	The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. [provided by RefSeq, Oct 2017].
Disorder MIM:	180069
Disorder Html:	Leber congenital amaurosis 2, 204100 (3); Retinitis pigmentosa 20, 613794 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017002027.1	XP_016857516.1	retinoid isomerohydrolase isoform X1
NM_000329.2	NP_000320.1	retinoid isomerohydrolase
NM_000329.3	NP_000320.1	retinoid isomerohydrolase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rpe65	NP_084263.2
	RPE65	XP_001095946.1
Danio rerio (zebrafish)	rpe65c	NP_001107125.1
Xenopus tropicalis (tropical clawed frog)	rpe65	NP_001120538.1
Pan troglodytes (chimpanzee)	RPE65	XP_001165086.1
Canis lupus familiaris (dog)	RPE65	NP_001003176.1
Gallus gallus (chicken)	RPE65	NP_990215.1
Homo sapiens (human)	RPE65	NP_000320.1
Bos taurus (cattle)	RPE65	NP_776878.1
Rattus norvegicus (Norway rat)	Rpe65	NP_446014.2
Danio rerio (zebrafish)	rpe65b	NP_001082902.2
Danio rerio (zebrafish)	rpe65a	NP_957045.1

Gene Ontology

The following RPE65 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPE65 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24603	NM_000329.3 Latest version!	Homo sapiens retinoid isomerohydrolase RPE65 (RPE65), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu90367	XM_017002027.1 Latest version!	Homo sapiens retinoid isomerohydrolase RPE65 (RPE65), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu24603	NM_000329.2	Homo sapiens RPE65, retinoid isomerohydrolase (RPE65), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPE65 ( NM_000329.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000320.1 Homo sapiens RPE65, retinoid isomerohydrolase (RPE65), mRNA.

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CloneID	OHu24603
Clone ID Related Accession (Same CDS sequence)	NM_000329.2 , NM_000329.3
Accession Version	NM_000329.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	retinoid isomerohydrolase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U18991.1 and AL139413.11. This sequence is a reference standard in the RefSeqGene project. On Jun 9, 2005 this sequence version replaced NM_000329.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U18991.1, SRR1660807.163057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2155984 [ECO:0000348] ##Evidence-Data-END##

ATGTCTATCC AGGTTGAGCA TCCTGCTGGT GGTTACAAGA AACTGTTTGA AACTGTGGAG 
GAACTGTCCT CGCCGCTCAC AGCTCATGTA ACAGGCAGGA TCCCCCTCTG GCTCACCGGC 
AGTCTCCTTC GATGTGGGCC AGGACTCTTT GAAGTTGGAT CTGAGCCATT TTACCACCTG 
TTTGATGGGC AAGCCCTCCT GCACAAGTTT GACTTTAAAG AAGGACATGT CACATACCAC 
AGAAGGTTCA TCCGCACTGA TGCTTACGTA CGGGCAATGA CTGAGAAAAG GATCGTCATA 
ACAGAATTTG GCACCTGTGC TTTCCCAGAT CCCTGCAAGA ATATATTTTC CAGGTTTTTT 
TCTTACTTTC GAGGAGTAGA GGTTACTGAC AATGCCCTTG TTAATGTCTA CCCAGTGGGG 
GAAGATTACT ACGCTTGCAC AGAGACCAAC TTTATTACAA AGATTAATCC AGAGACCTTG 
GAGACAATTA AGCAGGTTGA TCTTTGCAAC TATGTCTCTG TCAATGGGGC CACTGCTCAC 
CCCCACATTG AAAATGATGG AACCGTTTAC AATATTGGTA ATTGCTTTGG AAAAAATTTT 
TCAATTGCCT ACAACATTGT AAAGATCCCA CCACTGCAAG CAGACAAGGA AGATCCAATA 
AGCAAGTCAG AGATCGTTGT ACAATTCCCC TGCAGTGACC GATTCAAGCC ATCTTACGTT 
CATAGTTTTG GTCTGACTCC CAACTATATC GTTTTTGTGG AGACACCAGT CAAAATTAAC 
CTGTTCAAGT TCCTTTCTTC ATGGAGTCTT TGGGGAGCCA ACTACATGGA TTGTTTTGAG 
TCCAATGAAA CCATGGGGGT TTGGCTTCAT ATTGCTGACA AAAAAAGGAA AAAGTACCTC 
AATAATAAAT ACAGAACTTC TCCTTTCAAC CTCTTCCATC ACATCAACAC CTATGAAGAC 
AATGGGTTTC TGATTGTGGA TCTCTGCTGC TGGAAAGGAT TTGAGTTTGT TTATAATTAC 
TTATATTTAG CCAATTTACG TGAGAACTGG GAAGAGGTGA AAAAAAATGC CAGAAAGGCT 
CCCCAACCTG AAGTTAGGAG ATATGTACTT CCTTTGAATA TTGACAAGGC TGACACAGGC 
AAGAATTTAG TCACGCTCCC CAATACAACT GCCACTGCAA TTCTGTGCAG TGACGAGACT 
ATCTGGCTGG AGCCTGAAGT TCTCTTTTCA GGGCCTCGTC AAGCATTTGA GTTTCCTCAA 
ATCAATTACC AGAAGTATTG TGGGAAACCT TACACATATG CGTATGGACT TGGCTTGAAT 
CACTTTGTTC CAGATAGGCT CTGTAAGCTG AATGTCAAAA CTAAAGAAAC TTGGGTTTGG 
CAAGAGCCTG ATTCATACCC ATCAGAACCC ATCTTTGTTT CTCACCCAGA TGCCTTGGAA 
GAAGATGATG GTGTAGTTCT GAGTGTGGTG GTGAGCCCAG GAGCAGGACA AAAGCCTGCT 
TATCTCCTGA TTCTGAATGC CAAGGACTTA AGTGAAGTTG CCCGGGCTGA AGTGGAGATT 
AACATCCCTG TCACCTTTCA TGGACTGTTC AAAAAATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000320.1
CDS	55..1656
Translation	MSIQVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEVGSEPFYHLFDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITEFGTCAFPDPCKNIFSRFFSYFRGVEVTDNALVNVYPVGEDYYACTETNFITKINPETLETIKQVDLCNYVSVNGATAHPHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYLNNKYRTSPFNLFHHINTYEDNGFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS

Target ORF information:

RefSeq Version	NM_000329.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RPE65, retinoid isomerohydrolase (RPE65), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000329.2

ORF Insert Sequence:

ATGTCTATCC AGGTTGAGCA TCCTGCTGGT GGTTACAAGA AACTGTTTGA AACTGTGGAG 
GAACTGTCCT CGCCGCTCAC AGCTCATGTA ACAGGCAGGA TCCCCCTCTG GCTCACCGGC 
AGTCTCCTTC GATGTGGGCC AGGACTCTTT GAAGTTGGAT CTGAGCCATT TTACCACCTG 
TTTGATGGGC AAGCCCTCCT GCACAAGTTT GACTTTAAAG AAGGACATGT CACATACCAC 
AGAAGGTTCA TCCGCACTGA TGCTTACGTA CGGGCAATGA CTGAGAAAAG GATCGTCATA 
ACAGAATTTG GCACCTGTGC TTTCCCAGAT CCCTGCAAGA ATATATTTTC CAGGTTTTTT 
TCTTACTTTC GAGGAGTAGA GGTTACTGAC AATGCCCTTG TTAATGTCTA CCCAGTGGGG 
GAAGATTACT ACGCTTGCAC AGAGACCAAC TTTATTACAA AGATTAATCC AGAGACCTTG 
GAGACAATTA AGCAGGTTGA TCTTTGCAAC TATGTCTCTG TCAATGGGGC CACTGCTCAC 
CCCCACATTG AAAATGATGG AACCGTTTAC AATATTGGTA ATTGCTTTGG AAAAAATTTT 
TCAATTGCCT ACAACATTGT AAAGATCCCA CCACTGCAAG CAGACAAGGA AGATCCAATA 
AGCAAGTCAG AGATCGTTGT ACAATTCCCC TGCAGTGACC GATTCAAGCC ATCTTACGTT 
CATAGTTTTG GTCTGACTCC CAACTATATC GTTTTTGTGG AGACACCAGT CAAAATTAAC 
CTGTTCAAGT TCCTTTCTTC ATGGAGTCTT TGGGGAGCCA ACTACATGGA TTGTTTTGAG 
TCCAATGAAA CCATGGGGGT TTGGCTTCAT ATTGCTGACA AAAAAAGGAA AAAGTACCTC 
AATAATAAAT ACAGAACTTC TCCTTTCAAC CTCTTCCATC ACATCAACAC CTATGAAGAC 
AATGGGTTTC TGATTGTGGA TCTCTGCTGC TGGAAAGGAT TTGAGTTTGT TTATAATTAC 
TTATATTTAG CCAATTTACG TGAGAACTGG GAAGAGGTGA AAAAAAATGC CAGAAAGGCT 
CCCCAACCTG AAGTTAGGAG ATATGTACTT CCTTTGAATA TTGACAAGGC TGACACAGGC 
AAGAATTTAG TCACGCTCCC CAATACAACT GCCACTGCAA TTCTGTGCAG TGACGAGACT 
ATCTGGCTGG AGCCTGAAGT TCTCTTTTCA GGGCCTCGTC AAGCATTTGA GTTTCCTCAA 
ATCAATTACC AGAAGTATTG TGGGAAACCT TACACATATG CGTATGGACT TGGCTTGAAT 
CACTTTGTTC CAGATAGGCT CTGTAAGCTG AATGTCAAAA CTAAAGAAAC TTGGGTTTGG 
CAAGAGCCTG ATTCATACCC ATCAGAACCC ATCTTTGTTT CTCACCCAGA TGCCTTGGAA 
GAAGATGATG GTGTAGTTCT GAGTGTGGTG GTGAGCCCAG GAGCAGGACA AAAGCCTGCT 
TATCTCCTGA TTCTGAATGC CAAGGACTTA AGTGAAGTTG CCCGGGCTGA AGTGGAGATT 
AACATCCCTG TCACCTTTCA TGGACTGTTC AAAAAATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPE65 ( NM_000329.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000320.1 Homo sapiens retinoid isomerohydrolase RPE65 (RPE65), mRNA.

Price & Availability↑

CloneID	OHu24603
Clone ID Related Accession (Same CDS sequence)	NM_000329.2 , NM_000329.3
Accession Version	NM_000329.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	retinoid isomerohydrolase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U18991.1 and AL139413.11. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000329.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075036.2, U18991.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2155984 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262340.6/ ENSP00000262340.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTATCC AGGTTGAGCA TCCTGCTGGT GGTTACAAGA AACTGTTTGA AACTGTGGAG 
GAACTGTCCT CGCCGCTCAC AGCTCATGTA ACAGGCAGGA TCCCCCTCTG GCTCACCGGC 
AGTCTCCTTC GATGTGGGCC AGGACTCTTT GAAGTTGGAT CTGAGCCATT TTACCACCTG 
TTTGATGGGC AAGCCCTCCT GCACAAGTTT GACTTTAAAG AAGGACATGT CACATACCAC 
AGAAGGTTCA TCCGCACTGA TGCTTACGTA CGGGCAATGA CTGAGAAAAG GATCGTCATA 
ACAGAATTTG GCACCTGTGC TTTCCCAGAT CCCTGCAAGA ATATATTTTC CAGGTTTTTT 
TCTTACTTTC GAGGAGTAGA GGTTACTGAC AATGCCCTTG TTAATGTCTA CCCAGTGGGG 
GAAGATTACT ACGCTTGCAC AGAGACCAAC TTTATTACAA AGATTAATCC AGAGACCTTG 
GAGACAATTA AGCAGGTTGA TCTTTGCAAC TATGTCTCTG TCAATGGGGC CACTGCTCAC 
CCCCACATTG AAAATGATGG AACCGTTTAC AATATTGGTA ATTGCTTTGG AAAAAATTTT 
TCAATTGCCT ACAACATTGT AAAGATCCCA CCACTGCAAG CAGACAAGGA AGATCCAATA 
AGCAAGTCAG AGATCGTTGT ACAATTCCCC TGCAGTGACC GATTCAAGCC ATCTTACGTT 
CATAGTTTTG GTCTGACTCC CAACTATATC GTTTTTGTGG AGACACCAGT CAAAATTAAC 
CTGTTCAAGT TCCTTTCTTC ATGGAGTCTT TGGGGAGCCA ACTACATGGA TTGTTTTGAG 
TCCAATGAAA CCATGGGGGT TTGGCTTCAT ATTGCTGACA AAAAAAGGAA AAAGTACCTC 
AATAATAAAT ACAGAACTTC TCCTTTCAAC CTCTTCCATC ACATCAACAC CTATGAAGAC 
AATGGGTTTC TGATTGTGGA TCTCTGCTGC TGGAAAGGAT TTGAGTTTGT TTATAATTAC 
TTATATTTAG CCAATTTACG TGAGAACTGG GAAGAGGTGA AAAAAAATGC CAGAAAGGCT 
CCCCAACCTG AAGTTAGGAG ATATGTACTT CCTTTGAATA TTGACAAGGC TGACACAGGC 
AAGAATTTAG TCACGCTCCC CAATACAACT GCCACTGCAA TTCTGTGCAG TGACGAGACT 
ATCTGGCTGG AGCCTGAAGT TCTCTTTTCA GGGCCTCGTC AAGCATTTGA GTTTCCTCAA 
ATCAATTACC AGAAGTATTG TGGGAAACCT TACACATATG CGTATGGACT TGGCTTGAAT 
CACTTTGTTC CAGATAGGCT CTGTAAGCTG AATGTCAAAA CTAAAGAAAC TTGGGTTTGG 
CAAGAGCCTG ATTCATACCC ATCAGAACCC ATCTTTGTTT CTCACCCAGA TGCCTTGGAA 
GAAGATGATG GTGTAGTTCT GAGTGTGGTG GTGAGCCCAG GAGCAGGACA AAAGCCTGCT 
TATCTCCTGA TTCTGAATGC CAAGGACTTA AGTGAAGTTG CCCGGGCTGA AGTGGAGATT 
AACATCCCTG TCACCTTTCA TGGACTGTTC AAAAAATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000320.1
CDS	50..1651
Translation	MSIQVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEVGSEPFYHLFDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITEFGTCAFPDPCKNIFSRFFSYFRGVEVTDNALVNVYPVGEDYYACTETNFITKINPETLETIKQVDLCNYVSVNGATAHPHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYLNNKYRTSPFNLFHHINTYEDNGFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS

Target ORF information:

RefSeq Version	NM_000329.3
Organism	Homo sapiens(human)
Definition	Homo sapiens retinoid isomerohydrolase RPE65 (RPE65), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000329.3

ORF Insert Sequence:

ATGTCTATCC AGGTTGAGCA TCCTGCTGGT GGTTACAAGA AACTGTTTGA AACTGTGGAG 
GAACTGTCCT CGCCGCTCAC AGCTCATGTA ACAGGCAGGA TCCCCCTCTG GCTCACCGGC 
AGTCTCCTTC GATGTGGGCC AGGACTCTTT GAAGTTGGAT CTGAGCCATT TTACCACCTG 
TTTGATGGGC AAGCCCTCCT GCACAAGTTT GACTTTAAAG AAGGACATGT CACATACCAC 
AGAAGGTTCA TCCGCACTGA TGCTTACGTA CGGGCAATGA CTGAGAAAAG GATCGTCATA 
ACAGAATTTG GCACCTGTGC TTTCCCAGAT CCCTGCAAGA ATATATTTTC CAGGTTTTTT 
TCTTACTTTC GAGGAGTAGA GGTTACTGAC AATGCCCTTG TTAATGTCTA CCCAGTGGGG 
GAAGATTACT ACGCTTGCAC AGAGACCAAC TTTATTACAA AGATTAATCC AGAGACCTTG 
GAGACAATTA AGCAGGTTGA TCTTTGCAAC TATGTCTCTG TCAATGGGGC CACTGCTCAC 
CCCCACATTG AAAATGATGG AACCGTTTAC AATATTGGTA ATTGCTTTGG AAAAAATTTT 
TCAATTGCCT ACAACATTGT AAAGATCCCA CCACTGCAAG CAGACAAGGA AGATCCAATA 
AGCAAGTCAG AGATCGTTGT ACAATTCCCC TGCAGTGACC GATTCAAGCC ATCTTACGTT 
CATAGTTTTG GTCTGACTCC CAACTATATC GTTTTTGTGG AGACACCAGT CAAAATTAAC 
CTGTTCAAGT TCCTTTCTTC ATGGAGTCTT TGGGGAGCCA ACTACATGGA TTGTTTTGAG 
TCCAATGAAA CCATGGGGGT TTGGCTTCAT ATTGCTGACA AAAAAAGGAA AAAGTACCTC 
AATAATAAAT ACAGAACTTC TCCTTTCAAC CTCTTCCATC ACATCAACAC CTATGAAGAC 
AATGGGTTTC TGATTGTGGA TCTCTGCTGC TGGAAAGGAT TTGAGTTTGT TTATAATTAC 
TTATATTTAG CCAATTTACG TGAGAACTGG GAAGAGGTGA AAAAAAATGC CAGAAAGGCT 
CCCCAACCTG AAGTTAGGAG ATATGTACTT CCTTTGAATA TTGACAAGGC TGACACAGGC 
AAGAATTTAG TCACGCTCCC CAATACAACT GCCACTGCAA TTCTGTGCAG TGACGAGACT 
ATCTGGCTGG AGCCTGAAGT TCTCTTTTCA GGGCCTCGTC AAGCATTTGA GTTTCCTCAA 
ATCAATTACC AGAAGTATTG TGGGAAACCT TACACATATG CGTATGGACT TGGCTTGAAT 
CACTTTGTTC CAGATAGGCT CTGTAAGCTG AATGTCAAAA CTAAAGAAAC TTGGGTTTGG 
CAAGAGCCTG ATTCATACCC ATCAGAACCC ATCTTTGTTT CTCACCCAGA TGCCTTGGAA 
GAAGATGATG GTGTAGTTCT GAGTGTGGTG GTGAGCCCAG GAGCAGGACA AAAGCCTGCT 
TATCTCCTGA TTCTGAATGC CAAGGACTTA AGTGAAGTTG CCCGGGCTGA AGTGGAGATT 
AACATCCCTG TCACCTTTCA TGGACTGTTC AAAAAATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPE65 ( XM_017002027.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857516.1 Homo sapiens retinoid isomerohydrolase RPE65 (RPE65), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu90367
Clone ID Related Accession (Same CDS sequence)	XM_017002027.1
Accession Version	XM_017002027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	retinoid isomerohydrolase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAGA AAAGGATCGT CATAACAGAA TTTGGCACCT GTGCTTTCCC AGATCCCTGC 
AAGAATATAT TTTCCAGGTT TTTTTCTTAC TTTCGAGGAG TAGAGGTTAC TGACAATGCC 
CTTGTTAATG TCTACCCAGT GGGGGAAGAT TACTACGCTT GCACAGAGAC CAACTTTATT 
ACAAAGATTA ATCCAGAGAC CTTGGAGACA ATTAAGCAGG TTGATCTTTG CAACTATGTC 
TCTGTCAATG GGGCCACTGC TCACCCCCAC ATTGAAAATG ATGGAACCGT TTACAATATT 
GGTAATTGCT TTGGAAAAAA TTTTTCAATT GCCTACAACA TTGTAAAGAT CCCACCACTG 
CAAGCAGACA AGGAAGATCC AATAAGCAAG TCAGAGATCG TTGTACAATT CCCCTGCAGT 
GACCGATTCA AGCCATCTTA CGTTCATAGT TTTGGTCTGA CTCCCAACTA TATCGTTTTT 
GTGGAGACAC CAGTCAAAAT TAACCTGTTC AAGTTCCTTT CTTCATGGAG TCTTTGGGGA 
GCCAACTACA TGGATTGTTT TGAGTCCAAT GAAACCATGG GGGTTTGGCT TCATATTGCT 
GACAAAAAAA GGAAAAAGTA CCTCAATAAT AAATACAGAA CTTCTCCTTT CAACCTCTTC 
CATCACATCA ACACCTATGA AGACAATGGG TTTCTGATTG TGGATCTCTG CTGCTGGAAA 
GGATTTGAGT TTGTTTATAA TTACTTATAT TTAGCCAATT TACGTGAGAA CTGGGAAGAG 
GTGAAAAAAA ATGCCAGAAA GGCTCCCCAA CCTGAAGTTA GGAGATATGT ACTTCCTTTG 
AATATTGACA AGGCTGACAC AGGCAAGAAT TTAGTCACGC TCCCCAATAC AACTGCCACT 
GCAATTCTGT GCAGTGACGA GACTATCTGG CTGGAGCCTG AAGTTCTCTT TTCAGGGCCT 
CGTCAAGCAT TTGAGTTTCC TCAAATCAAT TACCAGAAGT ATTGTGGGAA ACCTTACACA 
TATGCGTATG GACTTGGCTT GAATCACTTT GTTCCAGATA GGCTCTGTAA GCTGAATGTC 
AAAACTAAAG AAACTTGGGT TTGGCAAGAG CCTGATTCAT ACCCATCAGA ACCCATCTTT 
GTTTCTCACC CAGATGCCTT GGAAGAAGAT GATGGTGTAG TTCTGAGTGT GGTGGTGAGC 
CCAGGAGCAG GACAAAAGCC TGCTTATCTC CTGATTCTGA ATGCCAAGGA CTTAAGTGAA 
GTTGCCCGGG CTGAAGTGGA GATTAACATC CCTGTCACCT TTCATGGACT GTTCAAAAAA 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857516.1
CDS	543..1868
Translation	MTEKRIVITEFGTCAFPDPCKNIFSRFFSYFRGVEVTDNALVNVYPVGEDYYACTETNFITKINPETLETIKQVDLCNYVSVNGATAHPHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYLNNKYRTSPFNLFHHINTYEDNGFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS

Target ORF information:

RefSeq Version	XM_017002027.1
Organism	Homo sapiens(human)
Definition	Homo sapiens retinoid isomerohydrolase RPE65 (RPE65), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002027.1

ORF Insert Sequence:

ATGACTGAGA AAAGGATCGT CATAACAGAA TTTGGCACCT GTGCTTTCCC AGATCCCTGC 
AAGAATATAT TTTCCAGGTT TTTTTCTTAC TTTCGAGGAG TAGAGGTTAC TGACAATGCC 
CTTGTTAATG TCTACCCAGT GGGGGAAGAT TACTACGCTT GCACAGAGAC CAACTTTATT 
ACAAAGATTA ATCCAGAGAC CTTGGAGACA ATTAAGCAGG TTGATCTTTG CAACTATGTC 
TCTGTCAATG GGGCCACTGC TCACCCCCAC ATTGAAAATG ATGGAACCGT TTACAATATT 
GGTAATTGCT TTGGAAAAAA TTTTTCAATT GCCTACAACA TTGTAAAGAT CCCACCACTG 
CAAGCAGACA AGGAAGATCC AATAAGCAAG TCAGAGATCG TTGTACAATT CCCCTGCAGT 
GACCGATTCA AGCCATCTTA CGTTCATAGT TTTGGTCTGA CTCCCAACTA TATCGTTTTT 
GTGGAGACAC CAGTCAAAAT TAACCTGTTC AAGTTCCTTT CTTCATGGAG TCTTTGGGGA 
GCCAACTACA TGGATTGTTT TGAGTCCAAT GAAACCATGG GGGTTTGGCT TCATATTGCT 
GACAAAAAAA GGAAAAAGTA CCTCAATAAT AAATACAGAA CTTCTCCTTT CAACCTCTTC 
CATCACATCA ACACCTATGA AGACAATGGG TTTCTGATTG TGGATCTCTG CTGCTGGAAA 
GGATTTGAGT TTGTTTATAA TTACTTATAT TTAGCCAATT TACGTGAGAA CTGGGAAGAG 
GTGAAAAAAA ATGCCAGAAA GGCTCCCCAA CCTGAAGTTA GGAGATATGT ACTTCCTTTG 
AATATTGACA AGGCTGACAC AGGCAAGAAT TTAGTCACGC TCCCCAATAC AACTGCCACT 
GCAATTCTGT GCAGTGACGA GACTATCTGG CTGGAGCCTG AAGTTCTCTT TTCAGGGCCT 
CGTCAAGCAT TTGAGTTTCC TCAAATCAAT TACCAGAAGT ATTGTGGGAA ACCTTACACA 
TATGCGTATG GACTTGGCTT GAATCACTTT GTTCCAGATA GGCTCTGTAA GCTGAATGTC 
AAAACTAAAG AAACTTGGGT TTGGCAAGAG CCTGATTCAT ACCCATCAGA ACCCATCTTT 
GTTTCTCACC CAGATGCCTT GGAAGAAGAT GATGGTGTAG TTCTGAGTGT GGTGGTGAGC 
CCAGGAGCAG GACAAAAGCC TGCTTATCTC CTGATTCTGA ATGCCAAGGA CTTAAGTGAA 
GTTGCCCGGG CTGAAGTGGA GATTAACATC CCTGTCACCT TTCATGGACT GTTCAAAAAA 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

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japanmarket@genscript.com

電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

RPE65 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報