RPA2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPA2
Entrez Gene ID	6118
Full Name	replication protein A2
Synonyms	REPA2,RP-A p32,RP-A p34,RPA32
General protein information	Preferred Names replication protein A2 Names replication protein A 32 kDa subunit RF-A protein 2 replication factor A protein 2 replication protein A 34 kDa subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p35.3
Summary	This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017].
Disorder MIM:	179836

mRNA and Protein(s)

mRNA	Protein	Name
NM_002946.5	NP_002937.1	replication protein A 32 kDa subunit isoform 1
NM_001355129.1	NP_001342058.1	replication protein A 32 kDa subunit isoform 4
NM_001297558.1	NP_001284487.1	replication protein A 32 kDa subunit isoform 3
NM_002946.4	NP_002937.1	replication protein A 32 kDa subunit isoform 1
NM_001355128.1	NP_001342057.1	replication protein A 32 kDa subunit isoform 2
XM_024448862.1	XP_024304630.1	replication protein A 32 kDa subunit isoform X1
NM_001286076.1	NP_001273005.1	replication protein A 32 kDa subunit isoform 2
XM_024448864.1	XP_024304632.1	replication protein A 32 kDa subunit isoform X2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RPA2	NP_610077.1
Saccharomyces cerevisiae (baker's yeast)	RFA2	NP_014087.1
Schizosaccharomyces pombe (fission yeast)	ssb2	NP_588227.2
Oryza sativa (rice)	Os02g0633400	NP_001047510.1
Pan troglodytes (chimpanzee)	RPA2	XP_003307967.1
Bos taurus (cattle)	RPA2	NP_001039449.1
Neurospora crassa	NCU07717	XP_961967.1
Oryza sativa (rice)	Os02g0829100	NP_001048604.1
Kluyveromyces lactis	KLLA0E07371g	XP_002999398.1
	RPA2	XP_002802309.1
Canis lupus familiaris (dog)	RPA2	XP_003638988.1
Mus musculus (house mouse)	Rpa2	NP_035414.3
Rattus norvegicus (Norway rat)	Rpa2	NP_067593.1
Eremothecium gossypii	AGOS_ADL176W	NP_983920.1
Magnaporthe oryzae (rice blast fungus)	MGG_07124	XP_003715317.1
Arabidopsis thaliana (thale cress)	RPA2	NP_565571.1
Arabidopsis thaliana (thale cress)	RPA32B	NP_566188.1
Homo sapiens (human)	RPA2	NP_002937.1
Gallus gallus (chicken)	RPA2	NP_001026063.1
Danio rerio (zebrafish)	rpa2	NP_571786.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008332	XP_317127.3
Xenopus tropicalis (tropical clawed frog)	rpa2	NP_001006795.1

Gene Ontology
Bibliography

Related articles in PubMed

Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity.
Kottemann MC, Conti BA, Lach FP, Smogorzewska A
Molecular cell69(1)24-35.e5(2018 Jan)

RPA-Mediated Recruitment of the E3 Ligase RFWD3 Is Vital for Interstrand Crosslink Repair and Human Health.
Feeney L, Mu?oz IM, Lachaud C, Toth R, Appleton PL, Schindler D, Rouse J
Molecular cell66(5)610-621.e4(2017 Jun)

RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination.
Inano S, Sato K, Katsuki Y, Kobayashi W, Tanaka H, Nakajima K, Nakada S, Miyoshi H, Knies K, Takaori-Kondo A, Schindler D, Ishiai M, Kurumizaka H, Takata M
Molecular cell66(5)622-634.e8(2017 Jun)

Replication protein A2 phosphorylation after DNA damage by the coordinated action of ataxia telangiectasia-mutated and DNA-dependent protein kinase.
Wang H, Guan J, Wang H, Perrault AR, Wang Y, Iliakis G
Cancer research61(23)8554-63(2001 Dec)

Functional analysis of the four DNA binding domains of replication protein A. The role of RPA2 in ssDNA binding.
Bastin-Shanower SA, Brill SJ
The Journal of biological chemistry276(39)36446-53(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Single point mutations in the RPA32 subunit of RPA that abolish interaction with RFWD3 also inhibit interstrand crossling repair, demonstrating that RPA-mediated RFWD3 recruitment to stalled replication forks is important for ICL repair.
Title: RPA-Mediated Recruitment of the E3 Ligase RFWD3 Is Vital for Interstrand Crosslink Repair and Human Health.

E3 ligase RFWD3 functions in timely removal and degradation of RPA and RAD51 to allow homologous recombination progression to subsequent steps following mitomycin C damage.
Title: RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination.

knockdown of RPA2 promoted formation of the menin-p65 complex and repressed the expression of NF-kappaB-mediated genes. RPA2 expression was induced via an E2F1-dependent mechanism in MCF7 and MDA-MB-231 cells treated with NF-kappaB activators, TNF-alpha or lipopolysaccharide (LPS).
Title: Upregulation of RPA2 promotes NF-?B activation in breast cancer by relieving the antagonistic function of menin on NF-?B-regulated transcription.

RPA, best known for its role in DNA replication and repair, recruits HIRA to promoters and enhancers and regulates deposition of newly synthesized H3.3 to these regulatory elements for gene regulation.
Title: RPA Interacts with HIRA and Regulates H3.3 Deposition at Gene Regulatory Elements in Mammalian Cells.

The authors show that Vpr can form a trimolecular complex with UNG2 and RPA32 and the positive effect of UNG2 and RPA32 on the reverse transcription process leading to optimal virus replication and dissemination between the primary target cells of HIV-1.
Title: Uracil DNA glycosylase interacts with the p32 subunit of the replication protein A complex to modulate HIV-1 reverse transcription for optimal virus dissemination.

The following RPA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28754	NM_001355128.1 Latest version!	Homo sapiens replication protein A2 (RPA2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu28754	NM_001286076.1 Latest version!	Homo sapiens replication protein A2 (RPA2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu28759	NM_001297558.1 Latest version!	Homo sapiens replication protein A2 (RPA2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu28764	NM_002946.5 Latest version!	Homo sapiens replication protein A2 (RPA2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu103330	NM_001355129.1 Latest version!	Homo sapiens replication protein A2 (RPA2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu105754	XM_024448864.1 Latest version!	Homo sapiens replication protein A2 (RPA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu60307	XM_024448862.1 Latest version!	Homo sapiens replication protein A2 (RPA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu28764	NM_002946.4	Homo sapiens replication protein A2 (RPA2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPA2 ( NM_001355128.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001342057.1 Homo sapiens replication protein A2 (RPA2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu28754
Clone ID Related Accession (Same CDS sequence)	NM_001355128.1 , NM_001286076.1
Accession Version	NM_001355128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	replication protein A 32 kDa subunit isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB025488.1, BM839986.1, BC001630.1 and BG107669.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.89482.1, SRR1660803.370964.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGCTG CACCCATGGA CGTTCGCCAG TGGGTTGACA CAGATGACAC CAGCAGTGAA 
AACACTGTGG TTCCTCCAGA AACATATGTG AAAGTGGCAG GCCACCTGAG ATCTTTTCAG 
AACAAAAAGA GCCTGGTAGC CTTTAAGATC ATGCCCCTGG AGGATATGAA TGAGTTCACC 
ACACATATTC TGGAAGTGAT CAATGCACAC ATGGTACTAA GCAAAGCCAA CAGCCAGCCC 
TCAGCAGGGA GAGCACCTAT CAGCAATCCA GGAATGAGTG AAGCAGGGAA CTTTGGTGGG 
AATAGCTTCA TGCCAGCAAA TGGCCTCACT GTGGCCCAAA ACCAGGTGTT GAATTTGATT 
AAGGCTTGTC CAAGACCTGA AGGGTTGAAC TTTCAGGATC TCAAGAACCA GCTGAAACAC 
ATGTCTGTAT CCTCAATCAA GCAAGCTGTG GATTTTCTGA GCAATGAGGG GCACATCTAT 
TCTACTGTGG ATGATGACCA TTTTAAATCC ACAGATGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342057.1
CDS	536..1060
Translation	MTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

Target ORF information:

RefSeq Version	NM_001355128.1
Organism	Homo sapiens(human)
Definition	Homo sapiens replication protein A2 (RPA2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355128.1

ORF Insert Sequence:

ATGACAGCTG CACCCATGGA CGTTCGCCAG TGGGTTGACA CAGATGACAC CAGCAGTGAA 
AACACTGTGG TTCCTCCAGA AACATATGTG AAAGTGGCAG GCCACCTGAG ATCTTTTCAG 
AACAAAAAGA GCCTGGTAGC CTTTAAGATC ATGCCCCTGG AGGATATGAA TGAGTTCACC 
ACACATATTC TGGAAGTGAT CAATGCACAC ATGGTACTAA GCAAAGCCAA CAGCCAGCCC 
TCAGCAGGGA GAGCACCTAT CAGCAATCCA GGAATGAGTG AAGCAGGGAA CTTTGGTGGG 
AATAGCTTCA TGCCAGCAAA TGGCCTCACT GTGGCCCAAA ACCAGGTGTT GAATTTGATT 
AAGGCTTGTC CAAGACCTGA AGGGTTGAAC TTTCAGGATC TCAAGAACCA GCTGAAACAC 
ATGTCTGTAT CCTCAATCAA GCAAGCTGTG GATTTTCTGA GCAATGAGGG GCACATCTAT 
TCTACTGTGG ATGATGACCA TTTTAAATCC ACAGATGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPA2 ( NM_001286076.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273005.1 Homo sapiens replication protein A2 (RPA2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28754
Clone ID Related Accession (Same CDS sequence)	NM_001355128.1 , NM_001286076.1
Accession Version	NM_001286076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-06
Organism	Homo sapiens(human)
Product	replication protein A 32 kDa subunit isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB025488.1, AK300691.1, AL109927.61, BC001630.1 and BG107669.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.233433.1, SRR1803616.72396.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGCTG CACCCATGGA CGTTCGCCAG TGGGTTGACA CAGATGACAC CAGCAGTGAA 
AACACTGTGG TTCCTCCAGA AACATATGTG AAAGTGGCAG GCCACCTGAG ATCTTTTCAG 
AACAAAAAGA GCCTGGTAGC CTTTAAGATC ATGCCCCTGG AGGATATGAA TGAGTTCACC 
ACACATATTC TGGAAGTGAT CAATGCACAC ATGGTACTAA GCAAAGCCAA CAGCCAGCCC 
TCAGCAGGGA GAGCACCTAT CAGCAATCCA GGAATGAGTG AAGCAGGGAA CTTTGGTGGG 
AATAGCTTCA TGCCAGCAAA TGGCCTCACT GTGGCCCAAA ACCAGGTGTT GAATTTGATT 
AAGGCTTGTC CAAGACCTGA AGGGTTGAAC TTTCAGGATC TCAAGAACCA GCTGAAACAC 
ATGTCTGTAT CCTCAATCAA GCAAGCTGTG GATTTTCTGA GCAATGAGGG GCACATCTAT 
TCTACTGTGG ATGATGACCA TTTTAAATCC ACAGATGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273005.1
CDS	794..1318
Translation	MTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

Target ORF information:

RefSeq Version	NM_001286076.1
Organism	Homo sapiens(human)
Definition	Homo sapiens replication protein A2 (RPA2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286076.1

ORF Insert Sequence:

ATGACAGCTG CACCCATGGA CGTTCGCCAG TGGGTTGACA CAGATGACAC CAGCAGTGAA 
AACACTGTGG TTCCTCCAGA AACATATGTG AAAGTGGCAG GCCACCTGAG ATCTTTTCAG 
AACAAAAAGA GCCTGGTAGC CTTTAAGATC ATGCCCCTGG AGGATATGAA TGAGTTCACC 
ACACATATTC TGGAAGTGAT CAATGCACAC ATGGTACTAA GCAAAGCCAA CAGCCAGCCC 
TCAGCAGGGA GAGCACCTAT CAGCAATCCA GGAATGAGTG AAGCAGGGAA CTTTGGTGGG 
AATAGCTTCA TGCCAGCAAA TGGCCTCACT GTGGCCCAAA ACCAGGTGTT GAATTTGATT 
AAGGCTTGTC CAAGACCTGA AGGGTTGAAC TTTCAGGATC TCAAGAACCA GCTGAAACAC 
ATGTCTGTAT CCTCAATCAA GCAAGCTGTG GATTTTCTGA GCAATGAGGG GCACATCTAT 
TCTACTGTGG ATGATGACCA TTTTAAATCC ACAGATGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPA2 ( NM_001297558.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284487.1 Homo sapiens replication protein A2 (RPA2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28759
Clone ID Related Accession (Same CDS sequence)	NM_001297558.1
Accession Version	NM_001297558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	replication protein A 32 kDa subunit isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB025488.1, BX333932.2, BC001630.1 and BQ006360.1. On Jul 3, 2014 this sequence version replaced XM_005245965.1. Transcript Variant: This variant (3) uses an alternate splice site in the 5' region and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.260102.1, ERR279847.814.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGAG GAGACAGGAA CAAGCGTAGC ATCCGTGGAT TCGAAAGCTA TGGCAGCTCC 
TCATACGGGG GAGCCGGCGG CTACACGCAG TCCCCGGGGG GCTTTGGATC GCCCGCACCT 
TCTCAAGCCG AAAAGAAATC AAGAGCCCGA GCCCAGCACA TTGTGCCCTG TACTATATCT 
CAGCTGCTTT CTGCCACTTT GGTTGATGAA GTGTTCAGAA TTGGGAATGT TGAGATTTCA 
CAGGTCACTA TTGTGGGGAT CATCAGACAT GCAGAGAAGG CTCCAACCAA CATTGTTTAC 
AAAATAGATG ACATGACAGC TGCACCCATG GACGTTCGCC AGTGGGTTGA CACAGATGAC 
ACCAGCAGTG AAAACACTGT GGTTCCTCCA GAAACATATG TGAAAGTGGC AGGCCACCTG 
AGATCTTTTC AGAACAAAAA GAGCCTGGTA GCCTTTAAGA TCATGCCCCT GGAGGATATG 
AATGAGTTCA CCACACATAT TCTGGAAGTG ATCAATGCAC ACATGGTACT AAGCAAAGCC 
AACAGCCAGC CCTCAGCAGG GAGAGCACCT ATCAGCAATC CAGGAATGAG TGAAGCAGGG 
AACTTTGGTG GGAATAGCTT CATGCCAGCA AATGGCCTCA CTGTGGCCCA AAACCAGGTG 
TTGAATTTGA TTAAGGCTTG TCCAAGACCT GAAGGGTTGA ACTTTCAGGA TCTCAAGAAC 
CAGCTGAAAC ACATGTCTGT ATCCTCAATC AAGCAAGCTG TGGATTTTCT GAGCAATGAG 
GGGCACATCT ATTCTACTGT GGATGATGAC CATTTTAAAT CCACAGATGC AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284487.1
CDS	138..974
Translation	MGRGDRNKRSIRGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

Target ORF information:

RefSeq Version	NM_001297558.1
Organism	Homo sapiens(human)
Definition	Homo sapiens replication protein A2 (RPA2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297558.1

ORF Insert Sequence:

ATGGGCAGAG GAGACAGGAA CAAGCGTAGC ATCCGTGGAT TCGAAAGCTA TGGCAGCTCC 
TCATACGGGG GAGCCGGCGG CTACACGCAG TCCCCGGGGG GCTTTGGATC GCCCGCACCT 
TCTCAAGCCG AAAAGAAATC AAGAGCCCGA GCCCAGCACA TTGTGCCCTG TACTATATCT 
CAGCTGCTTT CTGCCACTTT GGTTGATGAA GTGTTCAGAA TTGGGAATGT TGAGATTTCA 
CAGGTCACTA TTGTGGGGAT CATCAGACAT GCAGAGAAGG CTCCAACCAA CATTGTTTAC 
AAAATAGATG ACATGACAGC TGCACCCATG GACGTTCGCC AGTGGGTTGA CACAGATGAC 
ACCAGCAGTG AAAACACTGT GGTTCCTCCA GAAACATATG TGAAAGTGGC AGGCCACCTG 
AGATCTTTTC AGAACAAAAA GAGCCTGGTA GCCTTTAAGA TCATGCCCCT GGAGGATATG 
AATGAGTTCA CCACACATAT TCTGGAAGTG ATCAATGCAC ACATGGTACT AAGCAAAGCC 
AACAGCCAGC CCTCAGCAGG GAGAGCACCT ATCAGCAATC CAGGAATGAG TGAAGCAGGG 
AACTTTGGTG GGAATAGCTT CATGCCAGCA AATGGCCTCA CTGTGGCCCA AAACCAGGTG 
TTGAATTTGA TTAAGGCTTG TCCAAGACCT GAAGGGTTGA ACTTTCAGGA TCTCAAGAAC 
CAGCTGAAAC ACATGTCTGT ATCCTCAATC AAGCAAGCTG TGGATTTTCT GAGCAATGAG 
GGGCACATCT ATTCTACTGT GGATGATGAC CATTTTAAAT CCACAGATGC AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPA2 ( NM_002946.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002937.1 Homo sapiens replication protein A2 (RPA2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28764
Clone ID Related Accession (Same CDS sequence)	NM_002946.5 , NM_002946.4
Accession Version	NM_002946.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	replication protein A 32 kDa subunit isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB025488.1, BC001630.1 and BG107669.1. On Nov 23, 2018 this sequence version replaced NM_002946.4. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.28402.1, SRR3476690.384967.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373912.8/ ENSP00000363021.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGAACA GTGGATTCGA AAGCTATGGC AGCTCCTCAT ACGGGGGAGC CGGCGGCTAC 
ACGCAGTCCC CGGGGGGCTT TGGATCGCCC GCACCTTCTC AAGCCGAAAA GAAATCAAGA 
GCCCGAGCCC AGCACATTGT GCCCTGTACT ATATCTCAGC TGCTTTCTGC CACTTTGGTT 
GATGAAGTGT TCAGAATTGG GAATGTTGAG ATTTCACAGG TCACTATTGT GGGGATCATC 
AGACATGCAG AGAAGGCTCC AACCAACATT GTTTACAAAA TAGATGACAT GACAGCTGCA 
CCCATGGACG TTCGCCAGTG GGTTGACACA GATGACACCA GCAGTGAAAA CACTGTGGTT 
CCTCCAGAAA CATATGTGAA AGTGGCAGGC CACCTGAGAT CTTTTCAGAA CAAAAAGAGC 
CTGGTAGCCT TTAAGATCAT GCCCCTGGAG GATATGAATG AGTTCACCAC ACATATTCTG 
GAAGTGATCA ATGCACACAT GGTACTAAGC AAAGCCAACA GCCAGCCCTC AGCAGGGAGA 
GCACCTATCA GCAATCCAGG AATGAGTGAA GCAGGGAACT TTGGTGGGAA TAGCTTCATG 
CCAGCAAATG GCCTCACTGT GGCCCAAAAC CAGGTGTTGA ATTTGATTAA GGCTTGTCCA 
AGACCTGAAG GGTTGAACTT TCAGGATCTC AAGAACCAGC TGAAACACAT GTCTGTATCC 
TCAATCAAGC AAGCTGTGGA TTTTCTGAGC AATGAGGGGC ACATCTATTC TACTGTGGAT 
GATGACCATT TTAAATCCAC AGATGCAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002937.1
CDS	133..945
Translation	MWNSGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

Target ORF information:

RefSeq Version	NM_002946.5
Organism	Homo sapiens(human)
Definition	Homo sapiens replication protein A2 (RPA2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002946.5

ORF Insert Sequence:

ATGTGGAACA GTGGATTCGA AAGCTATGGC AGCTCCTCAT ACGGGGGAGC CGGCGGCTAC 
ACGCAGTCCC CGGGGGGCTT TGGATCGCCC GCACCTTCTC AAGCCGAAAA GAAATCAAGA 
GCCCGAGCCC AGCACATTGT GCCCTGTACT ATATCTCAGC TGCTTTCTGC CACTTTGGTT 
GATGAAGTGT TCAGAATTGG GAATGTTGAG ATTTCACAGG TCACTATTGT GGGGATCATC 
AGACATGCAG AGAAGGCTCC AACCAACATT GTTTACAAAA TAGATGACAT GACAGCTGCA 
CCCATGGACG TTCGCCAGTG GGTTGACACA GATGACACCA GCAGTGAAAA CACTGTGGTT 
CCTCCAGAAA CATATGTGAA AGTGGCAGGC CACCTGAGAT CTTTTCAGAA CAAAAAGAGC 
CTGGTAGCCT TTAAGATCAT GCCCCTGGAG GATATGAATG AGTTCACCAC ACATATTCTG 
GAAGTGATCA ATGCACACAT GGTACTAAGC AAAGCCAACA GCCAGCCCTC AGCAGGGAGA 
GCACCTATCA GCAATCCAGG AATGAGTGAA GCAGGGAACT TTGGTGGGAA TAGCTTCATG 
CCAGCAAATG GCCTCACTGT GGCCCAAAAC CAGGTGTTGA ATTTGATTAA GGCTTGTCCA 
AGACCTGAAG GGTTGAACTT TCAGGATCTC AAGAACCAGC TGAAACACAT GTCTGTATCC 
TCAATCAAGC AAGCTGTGGA TTTTCTGAGC AATGAGGGGC ACATCTATTC TACTGTGGAT 
GATGACCATT TTAAATCCAC AGATGCAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPA2 ( NM_002946.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002937.1 Homo sapiens replication protein A2 (RPA2), transcript variant 1, mRNA.

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CloneID	OHu28764
Clone ID Related Accession (Same CDS sequence)	NM_002946.5 , NM_002946.4
Accession Version	NM_002946.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-24
Organism	Homo sapiens(human)
Product	replication protein A 32 kDa subunit isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB025488.1, BC001630.1 and BG107669.1. On Oct 29, 2013 this sequence version replaced NM_002946.3. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.598795.1, BC021257.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGAACA GTGGATTCGA AAGCTATGGC AGCTCCTCAT ACGGGGGAGC CGGCGGCTAC 
ACGCAGTCCC CGGGGGGCTT TGGATCGCCC GCACCTTCTC AAGCCGAAAA GAAATCAAGA 
GCCCGAGCCC AGCACATTGT GCCCTGTACT ATATCTCAGC TGCTTTCTGC CACTTTGGTT 
GATGAAGTGT TCAGAATTGG GAATGTTGAG ATTTCACAGG TCACTATTGT GGGGATCATC 
AGACATGCAG AGAAGGCTCC AACCAACATT GTTTACAAAA TAGATGACAT GACAGCTGCA 
CCCATGGACG TTCGCCAGTG GGTTGACACA GATGACACCA GCAGTGAAAA CACTGTGGTT 
CCTCCAGAAA CATATGTGAA AGTGGCAGGC CACCTGAGAT CTTTTCAGAA CAAAAAGAGC 
CTGGTAGCCT TTAAGATCAT GCCCCTGGAG GATATGAATG AGTTCACCAC ACATATTCTG 
GAAGTGATCA ATGCACACAT GGTACTAAGC AAAGCCAACA GCCAGCCCTC AGCAGGGAGA 
GCACCTATCA GCAATCCAGG AATGAGTGAA GCAGGGAACT TTGGTGGGAA TAGCTTCATG 
CCAGCAAATG GCCTCACTGT GGCCCAAAAC CAGGTGTTGA ATTTGATTAA GGCTTGTCCA 
AGACCTGAAG GGTTGAACTT TCAGGATCTC AAGAACCAGC TGAAACACAT GTCTGTATCC 
TCAATCAAGC AAGCTGTGGA TTTTCTGAGC AATGAGGGGC ACATCTATTC TACTGTGGAT 
GATGACCATT TTAAATCCAC AGATGCAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002937.1
CDS	355..1167
Translation	MWNSGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

Target ORF information:

RefSeq Version	NM_002946.4
Organism	Homo sapiens(human)
Definition	Homo sapiens replication protein A2 (RPA2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002946.4

ORF Insert Sequence:

ATGTGGAACA GTGGATTCGA AAGCTATGGC AGCTCCTCAT ACGGGGGAGC CGGCGGCTAC 
ACGCAGTCCC CGGGGGGCTT TGGATCGCCC GCACCTTCTC AAGCCGAAAA GAAATCAAGA 
GCCCGAGCCC AGCACATTGT GCCCTGTACT ATATCTCAGC TGCTTTCTGC CACTTTGGTT 
GATGAAGTGT TCAGAATTGG GAATGTTGAG ATTTCACAGG TCACTATTGT GGGGATCATC 
AGACATGCAG AGAAGGCTCC AACCAACATT GTTTACAAAA TAGATGACAT GACAGCTGCA 
CCCATGGACG TTCGCCAGTG GGTTGACACA GATGACACCA GCAGTGAAAA CACTGTGGTT 
CCTCCAGAAA CATATGTGAA AGTGGCAGGC CACCTGAGAT CTTTTCAGAA CAAAAAGAGC 
CTGGTAGCCT TTAAGATCAT GCCCCTGGAG GATATGAATG AGTTCACCAC ACATATTCTG 
GAAGTGATCA ATGCACACAT GGTACTAAGC AAAGCCAACA GCCAGCCCTC AGCAGGGAGA 
GCACCTATCA GCAATCCAGG AATGAGTGAA GCAGGGAACT TTGGTGGGAA TAGCTTCATG 
CCAGCAAATG GCCTCACTGT GGCCCAAAAC CAGGTGTTGA ATTTGATTAA GGCTTGTCCA 
AGACCTGAAG GGTTGAACTT TCAGGATCTC AAGAACCAGC TGAAACACAT GTCTGTATCC 
TCAATCAAGC AAGCTGTGGA TTTTCTGAGC AATGAGGGGC ACATCTATTC TACTGTGGAT 
GATGACCATT TTAAATCCAC AGATGCAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPA2 ( NM_001355129.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001342058.1 Homo sapiens replication protein A2 (RPA2), transcript variant 5, mRNA.

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CloneID	OHu103330
Clone ID Related Accession (Same CDS sequence)	NM_001355129.1
Accession Version	NM_001355129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	replication protein A 32 kDa subunit isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB025488.1, CB155053.1, BC001630.1 and BG107669.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.263387.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGAACA GTAATGATGG GGGTGGATTC GAAAGCTATG GCAGCTCCTC ATACGGGGGA 
GCCGGCGGCT ACACGCAGTC CCCGGGGGGC TTTGGATCGC CCGCACCTTC TCAAGCCGAA 
AAGAAATCAA GAGCCCGAGC CCAGCACATT GTGCCCTGTA CTATATCTCA GCTGCTTTCT 
GCCACTTTGG TTGATGAAGT GTTCAGAATT GGGAATGTTG AGATTTCACA GGTCACTATT 
GTGGGGATCA TCAGACATGC AGAGAAGGCT CCAACCAACA TTGTTTACAA AATAGATGAC 
ATGACAGCTG CACCCATGGA CGTTCGCCAG TGGGTTGACA CAGATGACAC CAGCAGTGAA 
AACACTGTGG TTCCTCCAGA AACATATGTG AAAGTGGCAG GCCACCTGAG ATCTTTTCAG 
AACAAAAAGA GCCTGGTAGC CTTTAAGATC ATGCCCCTGG AGGATATGAA TGAGTTCACC 
ACACATATTC TGGAAGTGAT CAATGCACAC ATGGTACTAA GCAAAGCCAA CAGCCAGCCC 
TCAGCAGGGA GAGCACCTAT CAGCAATCCA GGAATGAGTG AAGCAGGGAA CTTTGGTGGG 
AATAGCTTCA TGCCAGCAAA TGGCCTCACT GTGGCCCAAA ACCAGGTGTT GAATTTGATT 
AAGGCTTGTC CAAGACCTGA AGGGTTGAAC TTTCAGGATC TCAAGAACCA GCTGAAACAC 
ATGTCTGTAT CCTCAATCAA GCAAGCTGTG GATTTTCTGA GCAATGAGGG GCACATCTAT 
TCTACTGTGG ATGATGACCA TTTTAAATCC ACAGATGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342058.1
CDS	355..1179
Translation	MWNSNDGGGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

Target ORF information:

RefSeq Version	NM_001355129.1
Organism	Homo sapiens(human)
Definition	Homo sapiens replication protein A2 (RPA2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355129.1

ORF Insert Sequence:

ATGTGGAACA GTAATGATGG GGGTGGATTC GAAAGCTATG GCAGCTCCTC ATACGGGGGA 
GCCGGCGGCT ACACGCAGTC CCCGGGGGGC TTTGGATCGC CCGCACCTTC TCAAGCCGAA 
AAGAAATCAA GAGCCCGAGC CCAGCACATT GTGCCCTGTA CTATATCTCA GCTGCTTTCT 
GCCACTTTGG TTGATGAAGT GTTCAGAATT GGGAATGTTG AGATTTCACA GGTCACTATT 
GTGGGGATCA TCAGACATGC AGAGAAGGCT CCAACCAACA TTGTTTACAA AATAGATGAC 
ATGACAGCTG CACCCATGGA CGTTCGCCAG TGGGTTGACA CAGATGACAC CAGCAGTGAA 
AACACTGTGG TTCCTCCAGA AACATATGTG AAAGTGGCAG GCCACCTGAG ATCTTTTCAG 
AACAAAAAGA GCCTGGTAGC CTTTAAGATC ATGCCCCTGG AGGATATGAA TGAGTTCACC 
ACACATATTC TGGAAGTGAT CAATGCACAC ATGGTACTAA GCAAAGCCAA CAGCCAGCCC 
TCAGCAGGGA GAGCACCTAT CAGCAATCCA GGAATGAGTG AAGCAGGGAA CTTTGGTGGG 
AATAGCTTCA TGCCAGCAAA TGGCCTCACT GTGGCCCAAA ACCAGGTGTT GAATTTGATT 
AAGGCTTGTC CAAGACCTGA AGGGTTGAAC TTTCAGGATC TCAAGAACCA GCTGAAACAC 
ATGTCTGTAT CCTCAATCAA GCAAGCTGTG GATTTTCTGA GCAATGAGGG GCACATCTAT 
TCTACTGTGG ATGATGACCA TTTTAAATCC ACAGATGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPA2 ( XM_024448864.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304632.1 Homo sapiens replication protein A2 (RPA2), transcript variant X2, mRNA.

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CloneID	OHu105754
Clone ID Related Accession (Same CDS sequence)	XM_024448864.1
Accession Version	XM_024448864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	replication protein A 32 kDa subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACA GTGGATTCGA AAGCTATGGC AGCTCCTCAT ACGGGGGAGC CGGCGGCTAC 
ACGCAGTCCC CGGGGGGCTT TGGATCGCCC GCACCTTCTC AAGCCGAAAA GAAATCAAGA 
GCCCGAGCCC AGCACATTGT GCCCTGTACT ATATCTCAGC TGCTTTCTGC CACTTTGGTT 
GATGAAGTGT TCAGAATTGG GAATGTTGAG ATTTCACAGG TCACTATTGT GGGGATCATC 
AGACATGCAG AGAAGGCTCC AACCAACATT GTTTACAAAA TAGATGACAT GACAGCTGCA 
CCCATGGACG TTCGCCAGTG GGTTGACACA GATAACAAAA AGAGCCTGGT AGCCTTTAAG 
ATCATGCCCC TGGAGGATAT GAATGAGTTC ACCACACATA TTCTGGAAGT GATCAATGCA 
CACATGGTAC TAAGCAAAGC CAACAGCCAG CCCTCAGCAG GGAGAGCACC TATCAGCAAT 
CCAGGAATGA GTGAAGCAGG GAACTTTGGT GGGAATAGCT TCATGCCAGC AAATGGCCTC 
ACTGTGGCCC AAAACCAGGT GTTGAATTTG ATTAAGGCTT GTCCAAGACC TGAAGGGTTG 
AACTTTCAGG ATCTCAAGAA CCAGCTGAAA CACATGTCTG TATCCTCAAT CAAGCAAGCT 
GTGGATTTTC TGAGCAATGA GGGGCACATC TATTCTACTG TGGATGATGA CCATTTTAAA 
TCCACAGATG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304632.1
CDS	342..1079
Translation	MWNSGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

Target ORF information:

RefSeq Version	XM_024448864.1
Organism	Homo sapiens(human)
Definition	Homo sapiens replication protein A2 (RPA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448864.1

ORF Insert Sequence:

ATGTGGAACA GTGGATTCGA AAGCTATGGC AGCTCCTCAT ACGGGGGAGC CGGCGGCTAC 
ACGCAGTCCC CGGGGGGCTT TGGATCGCCC GCACCTTCTC AAGCCGAAAA GAAATCAAGA 
GCCCGAGCCC AGCACATTGT GCCCTGTACT ATATCTCAGC TGCTTTCTGC CACTTTGGTT 
GATGAAGTGT TCAGAATTGG GAATGTTGAG ATTTCACAGG TCACTATTGT GGGGATCATC 
AGACATGCAG AGAAGGCTCC AACCAACATT GTTTACAAAA TAGATGACAT GACAGCTGCA 
CCCATGGACG TTCGCCAGTG GGTTGACACA GATAACAAAA AGAGCCTGGT AGCCTTTAAG 
ATCATGCCCC TGGAGGATAT GAATGAGTTC ACCACACATA TTCTGGAAGT GATCAATGCA 
CACATGGTAC TAAGCAAAGC CAACAGCCAG CCCTCAGCAG GGAGAGCACC TATCAGCAAT 
CCAGGAATGA GTGAAGCAGG GAACTTTGGT GGGAATAGCT TCATGCCAGC AAATGGCCTC 
ACTGTGGCCC AAAACCAGGT GTTGAATTTG ATTAAGGCTT GTCCAAGACC TGAAGGGTTG 
AACTTTCAGG ATCTCAAGAA CCAGCTGAAA CACATGTCTG TATCCTCAAT CAAGCAAGCT 
GTGGATTTTC TGAGCAATGA GGGGCACATC TATTCTACTG TGGATGATGA CCATTTTAAA 
TCCACAGATG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPA2 ( XM_024448862.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304630.1 Homo sapiens replication protein A2 (RPA2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu60307
Clone ID Related Accession (Same CDS sequence)	XM_024448862.1
Accession Version	XM_024448862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	replication protein A 32 kDa subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGAG GAGACAGGAA CAAGCGTAGC ATCCGTGGAT TCGAAAGCTA TGGCAGCTCC 
TCATACGGGG GAGCCGGCGG CTACACGCAG TCCCCGGGGG GCTTTGGATC GCCCGCACCT 
TCTCAAGCCG AAAAGAAATC AAGAGCCCGA GCCCAGCACA TTGTGCCCTG TACTATATCT 
CAGCTGCTTT CTGCCACTTT GGTTGATGAA GTGTTCAGAA TTGGGAATGT TGAGATTTCA 
CAGGTCACTA TTGTGGGGAT CATCAGACAT GCAGAGAAGG CTCCAACCAA CATTGTTTAC 
AAAATAGATG ACATGACAGC TGCACCCATG GACGTTCGCC AGTGGGTTGA CACAGATAAC 
AAAAAGAGCC TGGTAGCCTT TAAGATCATG CCCCTGGAGG ATATGAATGA GTTCACCACA 
CATATTCTGG AAGTGATCAA TGCACACATG GTACTAAGCA AAGCCAACAG CCAGCCCTCA 
GCAGGGAGAG CACCTATCAG CAATCCAGGA ATGAGTGAAG CAGGGAACTT TGGTGGGAAT 
AGCTTCATGC CAGCAAATGG CCTCACTGTG GCCCAAAACC AGGTGTTGAA TTTGATTAAG 
GCTTGTCCAA GACCTGAAGG GTTGAACTTT CAGGATCTCA AGAACCAGCT GAAACACATG 
TCTGTATCCT CAATCAAGCA AGCTGTGGAT TTTCTGAGCA ATGAGGGGCA CATCTATTCT 
ACTGTGGATG ATGACCATTT TAAATCCACA GATGCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304630.1
CDS	82..843
Translation	MGRGDRNKRSIRGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

Target ORF information:

RefSeq Version	XM_024448862.1
Organism	Homo sapiens(human)
Definition	Homo sapiens replication protein A2 (RPA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448862.1

ORF Insert Sequence:

ATGGGCAGAG GAGACAGGAA CAAGCGTAGC ATCCGTGGAT TCGAAAGCTA TGGCAGCTCC 
TCATACGGGG GAGCCGGCGG CTACACGCAG TCCCCGGGGG GCTTTGGATC GCCCGCACCT 
TCTCAAGCCG AAAAGAAATC AAGAGCCCGA GCCCAGCACA TTGTGCCCTG TACTATATCT 
CAGCTGCTTT CTGCCACTTT GGTTGATGAA GTGTTCAGAA TTGGGAATGT TGAGATTTCA 
CAGGTCACTA TTGTGGGGAT CATCAGACAT GCAGAGAAGG CTCCAACCAA CATTGTTTAC 
AAAATAGATG ACATGACAGC TGCACCCATG GACGTTCGCC AGTGGGTTGA CACAGATAAC 
AAAAAGAGCC TGGTAGCCTT TAAGATCATG CCCCTGGAGG ATATGAATGA GTTCACCACA 
CATATTCTGG AAGTGATCAA TGCACACATG GTACTAAGCA AAGCCAACAG CCAGCCCTCA 
GCAGGGAGAG CACCTATCAG CAATCCAGGA ATGAGTGAAG CAGGGAACTT TGGTGGGAAT 
AGCTTCATGC CAGCAAATGG CCTCACTGTG GCCCAAAACC AGGTGTTGAA TTTGATTAAG 
GCTTGTCCAA GACCTGAAGG GTTGAACTTT CAGGATCTCA AGAACCAGCT GAAACACATG 
TCTGTATCCT CAATCAAGCA AGCTGTGGAT TTTCTGAGCA ATGAGGGGCA CATCTATTCT 
ACTGTGGATG ATGACCATTT TAAATCCACA GATGCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity.
Molecular cell69(1)24-35.e5(2018 Jan)
Kottemann MC,Conti BA,Lach FP,Smogorzewska A

RPA-Mediated Recruitment of the E3 Ligase RFWD3 Is Vital for Interstrand Crosslink Repair and Human Health.
Molecular cell66(5)610-621.e4(2017 Jun)
Feeney L,Mu?oz IM,Lachaud C,Toth R,Appleton PL,Schindler D,Rouse J

RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination.
Molecular cell66(5)622-634.e8(2017 Jun)
Inano S,Sato K,Katsuki Y,Kobayashi W,Tanaka H,Nakajima K,Nakada S,Miyoshi H,Knies K,Takaori-Kondo A,Schindler D,Ishiai M,Kurumizaka H,Takata M

Replication protein A2 phosphorylation after DNA damage by the coordinated action of ataxia telangiectasia-mutated and DNA-dependent protein kinase.
Cancer research61(23)8554-63(2001 Dec)
Wang H,Guan J,Wang H,Perrault AR,Wang Y,Iliakis G

Functional analysis of the four DNA binding domains of replication protein A. The role of RPA2 in ssDNA binding.
The Journal of biological chemistry276(39)36446-53(2001 Sep)
Bastin-Shanower SA,Brill SJ

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RPA2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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